MD01G1058200.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009803
Physical Location & Seq
Reverse (-)
16254795 .. 16257382
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1058200.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGAACTGCCAGGTGATGGATCAGATGTGATCAGTCAAACGGAACTGACCCAAGAGCCACTTTTATGTGGACTTCCAGATGATATTGCTCTTTTCTGCCTAGCAAGGGTGCCTCGGAAGTACCATACCCTCCTAAAGTGTGTCTCAAAAAGATGGAGAGACTTGGTGTGTAGTGAAGAGTGGCATTCTTACCGTAGAAAGCATAAACTTGATGAGACTTGGATCTATGCTTTGTGCCGAGACAAGTTGGACCGCCTATGCTTTTATGTGTTGGATCCAAACTCATCGAGACGGTCTTGGAAGCTTGTTCATGGGCTTCCGCCTCGTATCATGAAGAGAAAGGGAATGGGTTTTGAAGTTATAGGAAATAAAGTTTACTTGTTGGGTGGTTGCGGTTGGTGCGAAGATGCTACTGATGAGGTCTACTGTTATGATGCTTCCCTGAATGCTTGGAGTGAAGCTTCTCCCTTGTCCACTGCTAGGTGCTACTTTGCTTGTGAAATATTGGATGACAAAATATATTCCATTGGCGGGTTGGGTTCAATTTCAAGCTATCCGCATTCTTGGGACATATATGACCCTTGCACCAATAGTTGGAAATTTCACTCAGACCCAAACATCGTTCCTGAAATTGAGGATTCTGTGGTCATGGCCGGGAAGATTTACATTCGTTGTGGCACTTCTGCTGTAACTTCTCATGTGCACGCAGTTGTTTATGAACCGTCAAGTGGGACATGGCAGCATGCAGATTCTGAAATGGTTTCAGGCTGGCGAGGTCCAGCAGTTGTCGTAGACGGAACCCTCTATGTGTTGGATCAGAGTTCAGGAACGAGGCTGATGATGTGGGAGAAGGAAACTAGGGAGTGGATCCCTGTCGGGAGGCTGTCATCGTTGCTTACTAGACCGCCCTGTCAACTCGTAGCTATTGGAAAGAAATTTTATGTTGTGGGAAAGGGGCTTAGCACAGTCATGTTTGATGTTGAAAATGCAGGGAATATGGAAGGGGTAATGGTTAGTTCTTCAATACCTAAATTGAATTCAAACATTGATGTAATCGGCTGTAAATGTTTATCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

360

Amino Acids

40.27

Weight (kDa)

6.01

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 60 8.2e-08 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 88 - 251 2.3e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 94 - 270 1.4e-09 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 97 - 199 3e-06 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 110 - 315 3.3e-25 FKB95, Kelch-repeats domain
Kelch_1 PF01344 113 - 157 1.7e-08 Kelch motif
Beta-prop_Calicin PF13964 116 - 274 1.7e-10 Calicin, beta-propeller domain
Kelch_1 PF01344 160 - 200 2.2e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 909
AccB1I GGYRCC 1 cut(s) 109
AccB7I CCANNNNNTGG 2 cut(s) 17, 594
AccI GTMKAC 2 cut(s) 423, 792
AciI CCGC 6 cut(s) 253, 320, 393, 531, 557, 905
AclWI GGATC 7 cut(s) 28, 230, 269, 282, 822, 862, 875
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 3 cut(s) 599, 935, 1036
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 6 cut(s) 17, 106, 480, 531, 594, 728
AgsI TTSAA 7 cut(s) 356, 543, 549, 983, 1023, 1036, 1041
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 4 cut(s) 304, 461, 552, 923
AluI AGCT 4 cut(s) 304, 461, 552, 923
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 5 cut(s) 148, 153, 209, 234, 283
Alw44I GTGCAC 1 cut(s) 701
AlwI GGATC 7 cut(s) 28, 230, 269, 282, 822, 862, 875
AlwNI CAGNNNCTG 1 cut(s) 752
AoxI GGCC 1 cut(s) 651
ApaLI GTGCAC 1 cut(s) 701
ApeKI GCWGC 1 cut(s) 739
ApoI RAATTY 3 cut(s) 599, 935, 1036
AspS9I GGNCC 2 cut(s) 250, 776
AsuC2I CCSGG 1 cut(s) 655
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 2 cut(s) 250, 776
BaeGI GKGCMC 1 cut(s) 705
BamHI GGATCC 2 cut(s) 274, 867
BanI GGYRCC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 705
BbvI GCAGC 1 cut(s) 751
BccI CCATC 2 cut(s) 11, 147
BciT130I CCWGG 1 cut(s) 12
BclI TGATCA 1 cut(s) 30
BcnI CCSGG 1 cut(s) 655
BcoDI GTCTC 5 cut(s) 148, 153, 209, 234, 283
BfaI CTAG 4 cut(s) 101, 480, 858, 900
BisI GCNGC 1 cut(s) 740
BlpI GCTNAGC 1 cut(s) 959
BlsI GCNGC 1 cut(s) 741
Bme1390I CCNGG 2 cut(s) 12, 655
Bme18I GGWCC 2 cut(s) 250, 776
BmgT120I GGNCC 2 cut(s) 250, 776
BmiI GGNNCC 4 cut(s) 111, 276, 799, 869
BmrFI CCNGG 2 cut(s) 12, 655
BmsI GCATC 2 cut(s) 397, 424
Bpu1102I GCTNAGC 1 cut(s) 959
BpuMI CCSGG 1 cut(s) 655
Bsa29I ATCGAT 1 cut(s) 1073
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 106, 480, 531, 594, 728
BseBI CCWGG 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 1073
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 1 cut(s) 514
BseLI CCNNNNNNNGG 6 cut(s) 17, 106, 480, 531, 594, 728
BseMII CTCAG 1 cut(s) 621
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 1 cut(s) 751
BshFI GGCC 1 cut(s) 653
BshNI GGYRCC 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 1073
BsiHKAI GWGCWC 1 cut(s) 705
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 2 cut(s) 581, 745
BslI CCNNNNNNNGG 6 cut(s) 17, 106, 480, 531, 594, 728
BsmAI GTCTC 5 cut(s) 148, 153, 209, 234, 283
BsmBI CGTCTC 1 cut(s) 283
BsmFI GGGAC 2 cut(s) 581, 745
BsmI GAATGC 3 cut(s) 184, 451, 559
BsnI GGCC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 705
Bsp143I GATC 6 cut(s) 20, 30, 222, 274, 814, 867
Bsp1720I GCTNAGC 1 cut(s) 959
BspACI CCGC 6 cut(s) 253, 320, 393, 531, 557, 905
BspANI GGCC 1 cut(s) 653
BspCNI CTCAG 1 cut(s) 620
BspDI ATCGAT 1 cut(s) 1073
BspHI TCATGA 1 cut(s) 330
BspLI GGNNCC 4 cut(s) 111, 276, 799, 869
BspPI GGATC 7 cut(s) 28, 230, 269, 282, 822, 862, 875
BspT107I GGYRCC 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 6 cut(s) 20, 30, 222, 274, 814, 867
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 5 cut(s) 194, 294, 428, 723, 967
Bst6I CTCTTC 2 cut(s) 171, 329
BstC8I GCNNGC 3 cut(s) 705, 744, 770
BstDEI CTNAG 2 cut(s) 607, 959
BstF5I GGATG 1 cut(s) 514
BstKTI GATC 6 cut(s) 23, 33, 225, 277, 817, 870
BstMAI GTCTC 5 cut(s) 148, 153, 209, 234, 283
BstMBI GATC 6 cut(s) 20, 30, 222, 274, 814, 867
BstMWI GCNNNNNNNGC 1 cut(s) 399
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 1 cut(s) 746
BstSCI CCNGG 2 cut(s) 10, 653
BstSLI GKGCMC 1 cut(s) 705
BstV1I GCAGC 1 cut(s) 751
BstX2I RGATCY 3 cut(s) 222, 274, 867
BstYI RGATCY 3 cut(s) 222, 274, 867
Bsu15I ATCGAT 1 cut(s) 1073
BsuRI GGCC 1 cut(s) 653
BsuTUI ATCGAT 1 cut(s) 1073
BtsCI GGATG 1 cut(s) 514
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 3 cut(s) 705, 744, 770
CaiI CAGNNNCTG 1 cut(s) 752
CciI TCATGA 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 250, 776
ClaI ATCGAT 1 cut(s) 1073
Csp6I GTAC 1 cut(s) 121
CviAII CATG 7 cut(s) 311, 331, 649, 698, 735, 743, 970
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 2 cut(s) 607, 959
DpnI GATC 6 cut(s) 22, 32, 224, 276, 816, 869
DpnII GATC 6 cut(s) 20, 30, 222, 274, 814, 867
DrdI GACNNNNNNGTC 1 cut(s) 909
DseDI GACNNNNNNGTC 1 cut(s) 909
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 2 cut(s) 171, 329
EarI CTCTTC 2 cut(s) 171, 329
EciI GGCGGA 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 250, 776
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 1 cut(s) 10
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 7 cut(s) 314, 334, 652, 701, 738, 746, 973
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 581, 745
FatI CATG 7 cut(s) 310, 330, 648, 697, 734, 742, 969
FauI CCCGC 1 cut(s) 524
FbaI TGATCA 1 cut(s) 30
FblI GTMKAC 2 cut(s) 423, 792
Fnu4HI GCNGC 1 cut(s) 740
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 1 cut(s) 740
FspBI CTAG 4 cut(s) 101, 480, 858, 900
GluI GCNGC 1 cut(s) 740
HaeIII GGCC 1 cut(s) 653
HapII CCGG 1 cut(s) 654
Hin1II CATG 7 cut(s) 314, 334, 652, 701, 738, 746, 973
HincII GTYRAC 1 cut(s) 914
HindII GTYRAC 1 cut(s) 914
HindIII AAGCTT 2 cut(s) 302, 459
HinfI GANTC 2 cut(s) 638, 749
HpaII CCGG 1 cut(s) 654
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 7 cut(s) 71, 376, 424, 474, 703, 793, 914
Hpy188I TCNGA 5 cut(s) 25, 117, 610, 754, 819
Hpy188III TCNNGA 6 cut(s) 77, 288, 331, 626, 825, 877
Hpy8I GTNNAC 7 cut(s) 71, 376, 424, 474, 703, 793, 914
HpyAV CCTTC 2 cut(s) 844, 995
HpyCH4III ACNGT 5 cut(s) 194, 294, 428, 723, 967
HpyCH4V TGCA 4 cut(s) 585, 703, 746, 989
HpyF10VI GCNNNNNNNGC 1 cut(s) 399
HpyF3I CTNAG 2 cut(s) 607, 959
Hsp92II CATG 7 cut(s) 314, 334, 652, 701, 738, 746, 973
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 6 cut(s) 20, 30, 222, 274, 814, 867
Lsp1109I GCAGC 1 cut(s) 751
LweI GCATC 2 cut(s) 397, 424
MaeI CTAG 4 cut(s) 101, 480, 858, 900
MaeIII GTNAC 1 cut(s) 688
MalI GATC 6 cut(s) 22, 32, 224, 276, 816, 869
MboI GATC 6 cut(s) 20, 30, 222, 274, 814, 867
MboII GAAGA 5 cut(s) 188, 346, 416, 670, 1011
MflI RGATCY 3 cut(s) 222, 274, 867
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 6 cut(s) 543, 599, 630, 935, 1031, 1036
MmeI TCCRAC 4 cut(s) 228, 252, 575, 792
MnlI CCTC 9 cut(s) 123, 140, 333, 412, 628, 767, 812, 825, 873
MslI CAYNNNNRTG 2 cut(s) 64, 968
MspI CCGG 1 cut(s) 654
MspR9I CCNGG 2 cut(s) 12, 655
Mva1269I GAATGC 3 cut(s) 184, 451, 559
MvaI CCWGG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 399
NciI CCSGG 1 cut(s) 655
NdeII GATC 6 cut(s) 20, 30, 222, 274, 814, 867
NlaIII CATG 7 cut(s) 314, 334, 652, 701, 738, 746, 973
NlaIV GGNNCC 4 cut(s) 111, 276, 799, 869
NmeAIII GCCGAG 1 cut(s) 263
NspI RCATGY 1 cut(s) 746
PaeI GCATGC 1 cut(s) 746
PagI TCATGA 1 cut(s) 330
PctI GAATGC 3 cut(s) 184, 451, 559
PfeI GAWTC 2 cut(s) 638, 749
PflMI CCANNNNNTGG 2 cut(s) 17, 594
PkrI GCNGC 1 cut(s) 741
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 4 cut(s) 111, 276, 799, 869
PspPI GGNCC 2 cut(s) 250, 776
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 3 cut(s) 222, 274, 867
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 2 cut(s) 64, 968
SatI GCNGC 1 cut(s) 740
Sau3AI GATC 6 cut(s) 20, 30, 222, 274, 814, 867
Sau96I GGNCC 2 cut(s) 250, 776
ScrFI CCNGG 2 cut(s) 12, 655
SduI GDGCHC 1 cut(s) 705
SetI ASST 9 cut(s) 16, 306, 423, 463, 485, 554, 778, 925, 1030
SfaNI GCATC 2 cut(s) 397, 424
SinI GGWCC 2 cut(s) 250, 776
SmiMI CAYNNNNRTG 2 cut(s) 64, 968
SphI GCATGC 1 cut(s) 746
Sse9I AATT 6 cut(s) 543, 599, 630, 935, 1031, 1036
SsiI CCGC 6 cut(s) 253, 320, 393, 531, 557, 905
SspI AATATT 1 cut(s) 504
SspMI CTAG 4 cut(s) 101, 480, 858, 900
StyD4I CCNGG 2 cut(s) 10, 653
TaaI ACNGT 5 cut(s) 194, 294, 428, 723, 967
TaqI TCGA 2 cut(s) 287, 1073
TasI AATT 6 cut(s) 543, 599, 630, 935, 1031, 1036
TfiI GAWTC 2 cut(s) 638, 749
TscAI CASTG 1 cut(s) 481
TseI GCWGC 1 cut(s) 739
TspDTI ATGAA 3 cut(s) 299, 347, 732
TspGWI ACGGA 2 cut(s) 56, 810
TspRI CASTG 1 cut(s) 481
Van91I CCANNNNNTGG 2 cut(s) 17, 594
VneI GTGCAC 1 cut(s) 701
VpaK11BI GGWCC 2 cut(s) 250, 776
XapI RAATTY 3 cut(s) 599, 935, 1036
XceI RCATGY 1 cut(s) 746
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XmiI GTMKAC 2 cut(s) 423, 792
XspI CTAG 4 cut(s) 101, 480, 858, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.