Rmu_sc0003115.1_g000034

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003115.1
OG0009803
Physical Location & Seq
Reverse (-)
141730 .. 152097
10368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003115.1_g000034.1.cds

Sequence Viewer

Length: 2355 bp
atggtggcatcatcaaaaagtcccttccccgatgaagccaaaggagaaagagtaccaccttcattgaattcatcagaaaaaggagagtatgttgttcgtgaaggggaggttgggattggggtttacttcatttgggaaggagaggctgaagttactgggtctgttaatgctgatgaagacaatcggccggagtttcagttgaagcgatatgattactttggttatggttttaaagcattaggtcatcaagctgatgtaattgcttcatctaagctgacatgcttggtgctaccccatgaacattattctctgctgcagccaaaatcaatctggcatgctgataaagctcttgagacatgctctcttgttgagaatatattgcatttggagccaattgaagcaaatatatttcaggggattactttaccggaggcaccaagatttggacaggttttcggaggacagttagttggacaggcgttggctgcagcaacaaaaactgttgattgtctcaaagtcgtccatagtttgcatgcacattttttgcttgtcggggatttcaacacaccaattatatatcaagttcaccgagtacgtgatggaaagagctttgctactcgaagagtagatgcaatacaaaaagggataaacatattcactttactggcttcctttcaaaaagaagaagacggttctgtccaccaggaagccataatgccatctgtgcctcccccagatacgctcccatcaatggaagaactgcgcgaaagacgcctttttgatccccgtcttccaagaacttatcgaatcaaggtagctgcaaaggagtttgtcccgtggccaattgagatacgattttgtgaacctagtagccggaccaatcagactaaatctccaccaagattgaggttctggtttagagcaaaaggaaaactttcagatgatcaggctttgcatagatgtgttgtggcttatgcttcagatttgatattcgctggtgttagtctgaatcctcaccgtagaagaggacataaaactagtcaagttagtttggaccatgcaatgtggtttcaccgcccccttagagctgatgactggttactacatgtgatagtgagtcccattgcctataatgcacgcggctatgtcacaggccaaatgttcaacaccaagggagagcttgttgtatcattgactcaagagtccctgaataggccaattagaccaactccggctgtcacatctaagttgaaattcacttactgcaacattagaatggaacagcctggtaatggaacagatgggatcagtcaaatggaaccggcccaacagccactgatatctggacttcctgatgacattgctcttttctgcatggcaagggttccccgaaagtaccatacagtcctaaagtgtgtttcaaaaagatggcaacacttggtgggcagtgaagagtggcattcctaccgtcgaaagcataatcttgatgagacttggatctatgctttgtgccgagacaaattggagcgtctttgcctgtatgtattggatccaaagtcatcaagaaggagttggaagcttgttaatgagcttcctcctcgcaccttgaagagaaaaggcatgggttttgaagtgctaggaaagaaagtttacttgttgggtggttgcggttggtgtgaagatgctacagatgaggtctactactatgatgcttccctgaacacttggagtgaggctactcccttatctattgctaggtgctattttgcctgtgaagtattagatgaaaaaatatattctattggtggattaggttcaaattcaagcgatccacattcttgggacatttatgatcctagcactaatagttggatatttcactcagacccaaacattgtccctgaaattgaggattctgtggtcatggatgggaaaatctatatccgctgtggcacttctgctgtatcttctcatgtctatgctgttgtttatgaaccatccagcggtacatggcagcatgcagatgctgatatggtgtctggctggcgaggtccggcagttgtaatagacgagaccctctatgtattggatcagagctcaggaactaggctgatgatgtggcagaaggagactagggaatggattccagttgggaggctgtcatccttgcttaccagaccaccctgtcaactcgtagctattgggaagagtatttatgttgtcggaaaggggcttagcacagtcatgtttgatgttgaaaatgcagggaatatggaaggggtaattgtgagttcttcaatacctaaattgaattctgatgatgatgtaatcagctgtaaatgtttgtccatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

784

Amino Acids

88.22

Weight (kDa)

6.67

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 695, 1201, 2184
AccB1I GGYRCC 1 cut(s) 433
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 1698
AccII CGCG 2 cut(s) 765, 1140
AciI CCGC 5 cut(s) 1075, 1140, 1668, 1945, 2004
AclWI GGATC 8 cut(s) 776, 1313, 1505, 1544, 1557, 1823, 1847, 2097
AcoI YGGCCR 2 cut(s) 185, 839
AcsI RAATTY 4 cut(s) 67, 1253, 1819, 2311
AcuI CTGAAG 2 cut(s) 168, 963
AcyI GRCGYC 1 cut(s) 772
AdeI CACNNNGTG 1 cut(s) 1441
AfaI GTAC 4 cut(s) 54, 594, 1397, 2008
AfiI CCNNNNNNNGG 5 cut(s) 443, 751, 1212, 1292, 2003
AflIII ACRYGT 1 cut(s) 1105
AhlI ACTAGT 1 cut(s) 1037
AjnI CCWGG 2 cut(s) 702, 1285
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
Alw21I GWGCWC 1 cut(s) 2099
Alw26I GTCTC 6 cut(s) 347, 515, 1484, 1509, 2066, 2123
AlwI GGATC 8 cut(s) 776, 1313, 1505, 1544, 1557, 1823, 1847, 2097
AlwNI CAGNNNCTG 2 cut(s) 1336, 2027
AoxI GGCC 5 cut(s) 185, 839, 1153, 1214, 1323
ApeKI GCWGC 6 cut(s) 313, 316, 485, 488, 818, 2014
ApoI RAATTY 4 cut(s) 67, 1253, 1819, 2311
Asp700I GAANNNNTTC 2 cut(s) 934, 2142
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 4 cut(s) 876, 1054, 1324, 2051
AsuHPI GGTGA 3 cut(s) 578, 1007, 1064
AvaII GGWCC 3 cut(s) 876, 1054, 2051
BalI TGGCCA 1 cut(s) 841
BamHI GGATCC 1 cut(s) 1549
BanI GGYRCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 2099
BbsI GAAGAC 3 cut(s) 183, 693, 782
Bbv12I GWGCWC 1 cut(s) 2099
BbvI GCAGC 6 cut(s) 300, 328, 472, 500, 805, 2026
BccI CCATC 7 cut(s) 593, 727, 754, 1295, 1422, 1922, 2005
BciT130I CCWGG 2 cut(s) 704, 1287
BclI TGATCA 1 cut(s) 943
BcoDI GTCTC 6 cut(s) 347, 515, 1484, 1509, 2066, 2123
BcuI ACTAGT 1 cut(s) 1037
BfaI CTAG 7 cut(s) 867, 1038, 1637, 1755, 1857, 2106, 2133
BfmI CTRYAG 3 cut(s) 314, 486, 1686
BisI GCNGC 7 cut(s) 314, 317, 486, 489, 819, 1141, 2015
BlpI GCTNAGC 1 cut(s) 2234
BlsI GCNGC 7 cut(s) 315, 318, 487, 490, 820, 1142, 2016
Bme1390I CCNGG 2 cut(s) 704, 1287
Bme18I GGWCC 3 cut(s) 876, 1054, 2051
BmgT120I GGNCC 4 cut(s) 876, 1054, 1324, 2051
BmiI GGNNCC 5 cut(s) 390, 435, 1320, 1386, 1551
BmrFI CCNGG 2 cut(s) 704, 1287
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 5 cut(s) 17, 619, 1672, 1699, 2014
BmuI ACTGGG 1 cut(s) 165
BpiI GAAGAC 3 cut(s) 183, 693, 782
BplI GAGNNNNNCTC 2 cut(s) 344, 376
Bpu10I CCTNAGC 1 cut(s) 2098
Bpu1102I GCTNAGC 1 cut(s) 2234
BpuEI CTTGAG 2 cut(s) 371, 1182
BsaAI YACGTR 1 cut(s) 596
BsaHI GRCGYC 1 cut(s) 772
BsaI GGTCTC 1 cut(s) 2066
BsaJI CCNNGG 2 cut(s) 836, 1170
BsaWI WCCGGW 1 cut(s) 427
Bsc4I CCNNNNNNNGG 5 cut(s) 443, 751, 1212, 1292, 2003
Bse118I RCCGGY 1 cut(s) 1321
Bse1I ACTGG 4 cut(s) 160, 669, 1100, 2147
Bse3DI GCAATG 3 cut(s) 1068, 1122, 1359
BseBI CCWGG 2 cut(s) 704, 1287
BseDI CCNNGG 2 cut(s) 836, 1170
BseGI GGATG 3 cut(s) 1933, 1997, 2162
BseLI CCNNNNNNNGG 5 cut(s) 443, 751, 1212, 1292, 2003
BseMI GCAATG 3 cut(s) 1068, 1122, 1359
BseMII CTCAG 2 cut(s) 1896, 2112
BseNI ACTGG 4 cut(s) 160, 669, 1100, 2147
BseRI GAGGAG 1 cut(s) 1587
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 6 cut(s) 300, 328, 472, 500, 805, 2026
Bsh1236I CGCG 2 cut(s) 765, 1140
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 5 cut(s) 187, 841, 1155, 1216, 1325
BshNI GGYRCC 1 cut(s) 433
BsiEI CGRYCG 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 2099
BsiSI CCGG 6 cut(s) 188, 428, 874, 1232, 1322, 2054
BslFI GGGAC 6 cut(s) 6, 818, 1104, 1189, 1856, 1883
BslI CCNNNNNNNGG 5 cut(s) 443, 751, 1212, 1292, 2003
BsmAI GTCTC 6 cut(s) 347, 515, 1484, 1509, 2066, 2123
BsmFI GGGAC 6 cut(s) 6, 818, 1104, 1189, 1856, 1883
BsmI GAATGC 1 cut(s) 1459
BsnI GGCC 5 cut(s) 187, 841, 1155, 1216, 1325
Bso31I GGTCTC 1 cut(s) 2066
Bsp1286I GDGCHC 1 cut(s) 2099
Bsp143I GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
Bsp1720I GCTNAGC 1 cut(s) 2234
BspACI CCGC 5 cut(s) 1075, 1140, 1668, 1945, 2004
BspANI GGCC 5 cut(s) 187, 841, 1155, 1216, 1325
BspCNI CTCAG 2 cut(s) 1895, 2111
BspFNI CGCG 2 cut(s) 765, 1140
BspLI GGNNCC 5 cut(s) 390, 435, 1320, 1386, 1551
BspMAI CTGCAG 2 cut(s) 318, 490
BspPI GGATC 8 cut(s) 776, 1313, 1505, 1544, 1557, 1823, 1847, 2097
BspT107I GGYRCC 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 2066
BsrDI GCAATG 3 cut(s) 1068, 1122, 1359
BsrFI RCCGGY 1 cut(s) 1321
BsrI ACTGG 4 cut(s) 160, 669, 1100, 2147
BssAI RCCGGY 1 cut(s) 1321
BssECI CCNNGG 2 cut(s) 836, 1170
BssMI GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
BssNI GRCGYC 1 cut(s) 772
BssT1I CCWWGG 1 cut(s) 1170
Bst2UI CCWGG 2 cut(s) 704, 1287
Bst4CI ACNGT 7 cut(s) 465, 502, 692, 1019, 1405, 1469, 2242
Bst6I CTCTTC 5 cut(s) 616, 1018, 1446, 1604, 2201
BstACI GRCGYC 1 cut(s) 772
BstBAI YACGTR 1 cut(s) 596
BstC8I GCNNGC 5 cut(s) 336, 534, 1138, 2019, 2045
BstDEI CTNAG 6 cut(s) 270, 1082, 1245, 1882, 2098, 2234
BstDSI CCRYGG 1 cut(s) 836
BstF5I GGATG 3 cut(s) 1933, 1997, 2162
BstFNI CGCG 2 cut(s) 765, 1140
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 8 cut(s) 784, 946, 1308, 1500, 1552, 1831, 1855, 2092
BstMAI GTCTC 6 cut(s) 347, 515, 1484, 1509, 2066, 2123
BstMBI GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 6 cut(s) 344, 388, 485, 724, 771, 1133
BstNI CCWGG 2 cut(s) 704, 1287
BstNSI RCATGY 6 cut(s) 282, 338, 360, 536, 1109, 2021
BstSCI CCNGG 2 cut(s) 702, 1285
BstSFI CTRYAG 3 cut(s) 314, 486, 1686
BstUI CGCG 2 cut(s) 765, 1140
BstV1I GCAGC 6 cut(s) 300, 328, 472, 500, 805, 2026
BstV2I GAAGAC 3 cut(s) 183, 693, 782
BstX2I RGATCY 2 cut(s) 1497, 1549
BstXI CCANNNNNNTGG 1 cut(s) 1839
BstYI RGATCY 2 cut(s) 1497, 1549
BstZI CGGCCG 1 cut(s) 185
BsuRI GGCC 5 cut(s) 187, 841, 1155, 1216, 1325
BtgI CCRYGG 1 cut(s) 836
BtsCI GGATG 3 cut(s) 1933, 1997, 2162
BtsI GCAGTG 1 cut(s) 1453
BtsIMutI CAGTG 2 cut(s) 1334, 1453
Cac8I GCNNGC 5 cut(s) 336, 534, 1138, 2019, 2045
CaiI CAGNNNCTG 2 cut(s) 1336, 2027
CfoI GCGC 1 cut(s) 765
Cfr10I RCCGGY 1 cut(s) 1321
Cfr13I GGNCC 4 cut(s) 876, 1054, 1324, 2051
CseI GACGC 2 cut(s) 780, 1517
Csp6I GTAC 4 cut(s) 53, 593, 1396, 2007
CspCI CAANNNNNGTGG 4 cut(s) 45, 80, 885, 920
CviQI GTAC 4 cut(s) 53, 593, 1396, 2007
DdeI CTNAG 6 cut(s) 270, 1082, 1245, 1882, 2098, 2234
DpnI GATC 8 cut(s) 783, 945, 1307, 1499, 1551, 1830, 1854, 2091
DpnII GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
DraI TTTAAA 1 cut(s) 232
DraIII CACNNNGTG 1 cut(s) 1441
DrdI GACNNNNNNGTC 3 cut(s) 695, 1201, 2184
DseDI GACNNNNNNGTC 3 cut(s) 695, 1201, 2184
EaeI YGGCCR 2 cut(s) 185, 839
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 5 cut(s) 616, 1018, 1446, 1604, 2201
EarI CTCTTC 5 cut(s) 616, 1018, 1446, 1604, 2201
Ecl136II GAGCTC 1 cut(s) 2097
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 1170
Eco24I GRGCYC 1 cut(s) 2099
Eco31I GGTCTC 1 cut(s) 2066
Eco32I GATATC 1 cut(s) 1341
Eco47I GGWCC 3 cut(s) 876, 1054, 2051
Eco52I CGGCCG 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 2097
Eco57I CTGAAG 2 cut(s) 168, 963
EcoICRI GAGCTC 1 cut(s) 2097
EcoRI GAATTC 2 cut(s) 67, 2311
EcoRII CCWGG 2 cut(s) 702, 1285
EcoRV GATATC 1 cut(s) 1341
EcoT14I CCWWGG 1 cut(s) 1170
EcoT38I GRGCYC 1 cut(s) 2099
ErhI CCWWGG 1 cut(s) 1170
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 6 cut(s) 6, 818, 1104, 1189, 1856, 1883
FbaI TGATCA 1 cut(s) 943
FblI GTMKAC 1 cut(s) 1698
Fnu4HI GCNGC 7 cut(s) 314, 317, 486, 489, 819, 1141, 2015
FokI GGATG 3 cut(s) 1940, 1984, 2149
FriOI GRGCYC 1 cut(s) 2099
Fsp4HI GCNGC 7 cut(s) 314, 317, 486, 489, 819, 1141, 2015
FspBI CTAG 7 cut(s) 867, 1038, 1637, 1755, 1857, 2106, 2133
GlaI GCGC 1 cut(s) 764
GluI GCNGC 7 cut(s) 314, 317, 486, 489, 819, 1141, 2015
HaeIII GGCC 5 cut(s) 187, 841, 1155, 1216, 1325
HapII CCGG 6 cut(s) 188, 428, 874, 1232, 1322, 2054
HgaI GACGC 2 cut(s) 780, 1517
HhaI GCGC 1 cut(s) 765
Hin1I GRCGYC 1 cut(s) 772
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HincII GTYRAC 1 cut(s) 2189
HindII GTYRAC 1 cut(s) 2189
HindIII AAGCTT 1 cut(s) 1577
HinfI GANTC 7 cut(s) 807, 1009, 1117, 1195, 1202, 1913, 2143
HpaII CCGG 6 cut(s) 188, 428, 874, 1232, 1322, 2054
HphI GGTGA 3 cut(s) 578, 1007, 1064
Hpy166II GTNNAC 7 cut(s) 124, 586, 700, 863, 1651, 1699, 2189
Hpy188III TCNNGA 8 cut(s) 98, 350, 1199, 1344, 1352, 1484, 1563, 2100
Hpy8I GTNNAC 7 cut(s) 124, 586, 700, 863, 1651, 1699, 2189
Hpy99I CGWCG 1 cut(s) 1473
HpyAV CCTTC 7 cut(s) 34, 69, 95, 131, 1560, 2119, 2270
HpyCH4III ACNGT 7 cut(s) 465, 502, 692, 1019, 1405, 1469, 2242
HpyCH4IV ACGT 1 cut(s) 595
HpyF10VI GCNNNNNNNGC 6 cut(s) 344, 388, 485, 724, 771, 1133
HpyF3I CTNAG 6 cut(s) 270, 1082, 1245, 1882, 2098, 2234
HpySE526I ACGT 1 cut(s) 595
Hsp92I GRCGYC 1 cut(s) 772
HspAI GCGC 1 cut(s) 763
Ksp22I TGATCA 1 cut(s) 943
Kzo9I GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
LmnI GCTCC 3 cut(s) 388, 747, 1525
Lsp1109I GCAGC 6 cut(s) 300, 328, 472, 500, 805, 2026
LweI GCATC 5 cut(s) 17, 619, 1672, 1699, 2014
MaeI CTAG 7 cut(s) 867, 1038, 1637, 1755, 1857, 2106, 2133
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 4 cut(s) 151, 1098, 1147, 1237
MalI GATC 8 cut(s) 783, 945, 1307, 1499, 1551, 1830, 1854, 2091
MboI GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
MfeI CAATTG 2 cut(s) 393, 843
MflI RGATCY 2 cut(s) 1497, 1549
MhlI GDGCHC 1 cut(s) 2099
MlsI TGGCCA 1 cut(s) 841
MluNI TGGCCA 1 cut(s) 841
MlyI GAGTC 3 cut(s) 1126, 1189, 1211
MmeI TCCRAC 4 cut(s) 451, 1553, 1850, 2203
Mox20I TGGCCA 1 cut(s) 841
MroXI GAANNNNTTC 2 cut(s) 934, 2142
MscI TGGCCA 1 cut(s) 841
MseI TTAA 3 cut(s) 165, 231, 1584
MslI CAYNNNNRTG 5 cut(s) 960, 1274, 1977, 2022, 2243
Msp20I TGGCCA 1 cut(s) 841
MspA1I CMGCKG 3 cut(s) 1947, 2004, 2334
MspI CCGG 6 cut(s) 188, 428, 874, 1232, 1322, 2054
MspR9I CCNGG 2 cut(s) 704, 1287
MunI CAATTG 2 cut(s) 393, 843
Mva1269I GAATGC 1 cut(s) 1459
MvaI CCWGG 2 cut(s) 704, 1287
MvnI CGCG 2 cut(s) 765, 1140
MwoI GCNNNNNNNGC 6 cut(s) 344, 388, 485, 724, 771, 1133
NdeII GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
NlaIV GGNNCC 5 cut(s) 390, 435, 1320, 1386, 1551
NmeAIII GCCGAG 1 cut(s) 1538
NmuCI GTSAC 2 cut(s) 1147, 1237
NspI RCATGY 6 cut(s) 282, 338, 360, 536, 1109, 2021
PaeI GCATGC 3 cut(s) 338, 536, 2021
PciI ACATGT 1 cut(s) 1105
PctI GAATGC 1 cut(s) 1459
PdmI GAANNNNTTC 2 cut(s) 934, 2142
PfeI GAWTC 4 cut(s) 807, 1009, 1913, 2143
PflMI CCANNNNNTGG 1 cut(s) 443
PkrI GCNGC 7 cut(s) 315, 318, 487, 490, 820, 1142, 2016
PleI GAGTC 3 cut(s) 1125, 1189, 1210
PpsI GAGTC 3 cut(s) 1125, 1189, 1210
Ppu21I YACGTR 1 cut(s) 596
PscI ACATGT 1 cut(s) 1105
Psp124BI GAGCTC 1 cut(s) 2099
Psp6I CCWGG 2 cut(s) 702, 1285
PspGI CCWGG 2 cut(s) 702, 1285
PspN4I GGNNCC 5 cut(s) 390, 435, 1320, 1386, 1551
PspPI GGNCC 4 cut(s) 876, 1054, 1324, 2051
PstI CTGCAG 2 cut(s) 318, 490
PstNI CAGNNNCTG 2 cut(s) 1336, 2027
PsuI RGATCY 2 cut(s) 1497, 1549
PvuII CAGCTG 1 cut(s) 2334
RsaI GTAC 4 cut(s) 54, 594, 1397, 2008
RsaNI GTAC 4 cut(s) 53, 593, 1396, 2007
RseI CAYNNNNRTG 5 cut(s) 960, 1274, 1977, 2022, 2243
SacI GAGCTC 1 cut(s) 2099
SaqAI TTAA 3 cut(s) 165, 231, 1584
SatI GCNGC 7 cut(s) 314, 317, 486, 489, 819, 1141, 2015
Sau3AI GATC 8 cut(s) 781, 943, 1305, 1497, 1549, 1828, 1852, 2089
Sau96I GGNCC 4 cut(s) 876, 1054, 1324, 2051
SchI GAGTC 3 cut(s) 1126, 1189, 1211
ScrFI CCNGG 2 cut(s) 704, 1287
SduI GDGCHC 1 cut(s) 2099
SfaNI GCATC 5 cut(s) 17, 619, 1672, 1699, 2014
SfcI CTRYAG 3 cut(s) 314, 486, 1686
SinI GGWCC 3 cut(s) 876, 1054, 2051
SmiMI CAYNNNNRTG 5 cut(s) 960, 1274, 1977, 2022, 2243
SmlI CTYRAG 2 cut(s) 350, 1197
SmoI CTYRAG 2 cut(s) 350, 1197
SpeI ACTAGT 1 cut(s) 1037
SphI GCATGC 3 cut(s) 338, 536, 2021
SsiI CCGC 5 cut(s) 1075, 1140, 1668, 1945, 2004
SspMI CTAG 7 cut(s) 867, 1038, 1637, 1755, 1857, 2106, 2133
SstI GAGCTC 1 cut(s) 2099
StyD4I CCNGG 2 cut(s) 702, 1285
StyI CCWWGG 1 cut(s) 1170
TaaI ACNGT 7 cut(s) 465, 502, 692, 1019, 1405, 1469, 2242
TaiI ACGT 1 cut(s) 598
TaqI TCGA 3 cut(s) 619, 805, 1471
TauI GCSGC 1 cut(s) 1143
TfiI GAWTC 4 cut(s) 807, 1009, 1913, 2143
Tru1I TTAA 3 cut(s) 165, 231, 1584
Tru9I TTAA 3 cut(s) 165, 231, 1584
TscAI CASTG 2 cut(s) 1341, 1453
TseFI GTSAC 2 cut(s) 1147, 1237
TseI GCWGC 6 cut(s) 313, 316, 485, 488, 818, 2014
Tsp45I GTSAC 2 cut(s) 1147, 1237
TspDTI ATGAA 9 cut(s) 48, 51, 60, 118, 189, 255, 312, 1800, 2007
TspRI CASTG 2 cut(s) 1341, 1453
Van91I CCANNNNNTGG 1 cut(s) 443
VpaK11BI GGWCC 3 cut(s) 876, 1054, 2051
XapI RAATTY 4 cut(s) 67, 1253, 1819, 2311
XceI RCATGY 6 cut(s) 282, 338, 360, 536, 1109, 2021
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
XmiI GTMKAC 1 cut(s) 1698
XmnI GAANNNNTTC 2 cut(s) 934, 2142
XspI CTAG 7 cut(s) 867, 1038, 1637, 1755, 1857, 2106, 2133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.