Rw1G022720

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009803
Physical Location & Seq
Reverse (-)
48095625 .. 48101685
6061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G022720.1

Sequence Viewer

Length: 1287 bp
ATGGACTCCGAATCAGTGATTGAGTTTCTGGGCTGCGTTCCTCTGCTTCAGAGACTACCCGGCTCCTCGCTCAAGAAAATCGCCCAGCTTGTAATTTTAAAGCACTACGAAAAAGGAGAGTATGTTGTTCGTGAAGGGGAGGTTGGGATTGGGGTTTACTTCATTTGGGAAGGAGAGGCTGAAGTTACTGGGTCTGTTAATGCTGATGAAGACAATCGGCCGGAGTTTCAGTTGAAGCGATATGATTACTTTGGTTATGGTTTTAAAGCATTAGGTCATCAAGCTGATGTAATTGCTTCATCTAAGCTGACATGCTTGGTGCTACCCCATGAACATTATTCTCTGCTGCAGCCAAAATCAATCTGGCATGCTGATAAAGCTCTTGAGACATGCTCTCTTGTTGAGAATATATTGCATTTGGAGCCAATTGAAGCAAATATATTTCAGGGGATTACTTTACCGGAGGCACCAAGATTTGGACAGGTTTTCGGAGGACAGTTAGTTGGACAGGCGTTGGCTGCAGCAACAAAAACTGTTGATTGTCTCAAAGTCGTCCATAGTTTGCATGCACATTTTTTGCTTGTCGGGGATTTCAACACACCAATTATATATCAAGTTCACCGAGTACGTGATGGAAAGAGCTTTGCTACTCGAAGAGTAGATGCAATACAAAAAGGGATAAACATATTCACTTTACTGGCTTCCTTTCAAAAAGAAGAAGACGGTTCTGTCCACCAGGAAGCCATAATGCCATCTGTGCCTCCCCCAGATACGCTCCCATCAATGGAAGAACTGCGCGAAAGACGCCTTTTTGATCCCCGTCTTCCAAGAACTTATCGAATCAAGGTAGCTGCAAAGGAGTTTGTCCCGTGGCCAATTGAGATACGATTTTGTGAACCTAGTAGCCGGACCAATCAGACTAAATCTCCACCAAGATTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCAGATGATCAGGCTTTGCATAGATGTGTTGTGGCTTATGCTTCAGATTTGATATTCGCTGGTGTTAGTCTGAATCCTCACCGTAGAAGAGGACATAAAACTAGTCAAGTTAGTTTGGACCATGCAATGTGGTTTCACCGCCCCCTTAGAGCTGATGACTGGTTACTACATGTGATAGTGAGTCCCATTGCCTATAATGCACGCGGCTATGTCACAGGCCAAATGTTCAACACCAAGGGAGAGCTTGTTGTATCATTGACTCAAGAGTCCCTGAATAGGCCAATTAGACCAACTCCGGCTGTCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

428

Amino Acids

48.33

Weight (kDa)

8.61

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 34 - 112 3.1e-08 Cyclic nucleotide-binding domain
4HBT_3 PF13622 158 - 237 8.3e-18 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 302 - 413 1.9e-23 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 306 - 412 5.6e-24 Acyl-CoA thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 728, 1234
AccB1I GGYRCC 1 cut(s) 466
AccB7I CCANNNNNTGG 1 cut(s) 476
AccII CGCG 2 cut(s) 798, 1173
AciI CCGC 2 cut(s) 1108, 1173
AclWI GGATC 1 cut(s) 809
AcoI YGGCCR 2 cut(s) 218, 872
AcuI CTGAAG 3 cut(s) 32, 201, 996
AcyI GRCGYC 1 cut(s) 805
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 3 cut(s) 476, 784, 1245
AflIII ACRYGT 1 cut(s) 1138
AgsI TTSAA 5 cut(s) 235, 431, 595, 710, 1198
AhlI ACTAGT 1 cut(s) 1070
AjnI CCWGG 1 cut(s) 735
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 8 cut(s) 88, 284, 307, 380, 642, 851, 1121, 1213
AluI AGCT 8 cut(s) 88, 284, 307, 380, 642, 851, 1121, 1213
Alw26I GTCTC 3 cut(s) 46, 380, 548
AlwI GGATC 1 cut(s) 809
AoxI GGCC 4 cut(s) 218, 872, 1186, 1247
ApeKI GCWGC 6 cut(s) 33, 346, 349, 518, 521, 851
Asp700I GAANNNNTTC 1 cut(s) 967
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 2 cut(s) 909, 1087
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 611, 1040, 1097
AvaII GGWCC 2 cut(s) 909, 1087
BalI TGGCCA 1 cut(s) 874
BanI GGYRCC 1 cut(s) 466
BbsI GAAGAC 3 cut(s) 216, 726, 815
BbvI GCAGC 6 cut(s) 20, 333, 361, 505, 533, 838
BccI CCATC 3 cut(s) 626, 760, 787
BciT130I CCWGG 1 cut(s) 737
BclI TGATCA 1 cut(s) 976
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 3 cut(s) 46, 380, 548
BcuI ACTAGT 1 cut(s) 1070
BfaI CTAG 2 cut(s) 900, 1071
BfmI CTRYAG 2 cut(s) 347, 519
BisI GCNGC 7 cut(s) 34, 347, 350, 519, 522, 852, 1174
BlsI GCNGC 7 cut(s) 35, 348, 351, 520, 523, 853, 1175
Bme1390I CCNGG 2 cut(s) 60, 737
Bme18I GGWCC 2 cut(s) 909, 1087
BmgT120I GGNCC 2 cut(s) 909, 1087
BmiI GGNNCC 3 cut(s) 64, 423, 468
BmrFI CCNGG 2 cut(s) 60, 737
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 1 cut(s) 652
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 3 cut(s) 216, 726, 815
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpuEI CTTGAG 3 cut(s) 56, 404, 1215
BpuMI CCSGG 1 cut(s) 60
BsaAI YACGTR 1 cut(s) 629
BsaHI GRCGYC 1 cut(s) 805
BsaJI CCNNGG 2 cut(s) 869, 1203
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 8 cut(s) 108, 138, 215, 245, 910, 940, 1200, 1230
Bsc4I CCNNNNNNNGG 3 cut(s) 476, 784, 1245
Bse1I ACTGG 3 cut(s) 193, 702, 1133
Bse3DI GCAATG 2 cut(s) 1101, 1155
BseBI CCWGG 1 cut(s) 737
BseDI CCNNGG 2 cut(s) 869, 1203
BseLI CCNNNNNNNGG 3 cut(s) 476, 784, 1245
BseMI GCAATG 2 cut(s) 1101, 1155
BseNI ACTGG 3 cut(s) 193, 702, 1133
BseRI GAGGAG 1 cut(s) 55
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 6 cut(s) 20, 333, 361, 505, 533, 838
BseYI CCCAGC 1 cut(s) 84
Bsh1236I CGCG 2 cut(s) 798, 1173
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 4 cut(s) 220, 874, 1188, 1249
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 221
BsiSI CCGG 5 cut(s) 60, 221, 461, 907, 1265
BslFI GGGAC 3 cut(s) 851, 1137, 1222
BslI CCNNNNNNNGG 3 cut(s) 476, 784, 1245
BsmAI GTCTC 3 cut(s) 46, 380, 548
BsmFI GGGAC 3 cut(s) 851, 1137, 1222
BsnI GGCC 4 cut(s) 220, 874, 1188, 1249
Bsp143I GATC 2 cut(s) 814, 976
BspACI CCGC 2 cut(s) 1108, 1173
BspANI GGCC 4 cut(s) 220, 874, 1188, 1249
BspFNI CGCG 2 cut(s) 798, 1173
BspLI GGNNCC 3 cut(s) 64, 423, 468
BspMAI CTGCAG 2 cut(s) 351, 523
BspPI GGATC 1 cut(s) 809
BspT107I GGYRCC 1 cut(s) 466
BsrDI GCAATG 2 cut(s) 1101, 1155
BsrI ACTGG 3 cut(s) 193, 702, 1133
BssECI CCNNGG 2 cut(s) 869, 1203
BssMI GATC 2 cut(s) 814, 976
BssNI GRCGYC 1 cut(s) 805
BssT1I CCWWGG 1 cut(s) 1203
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 4 cut(s) 498, 535, 725, 1052
Bst6I CTCTTC 2 cut(s) 649, 1051
BstACI GRCGYC 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 3 cut(s) 369, 567, 1171
BstDEI CTNAG 3 cut(s) 303, 1115, 1278
BstDSI CCRYGG 1 cut(s) 869
BstFNI CGCG 2 cut(s) 798, 1173
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 2 cut(s) 817, 979
BstMAI GTCTC 3 cut(s) 46, 380, 548
BstMBI GATC 2 cut(s) 814, 976
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 6 cut(s) 377, 421, 518, 757, 804, 1166
BstNI CCWGG 1 cut(s) 737
BstNSI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
BstSCI CCNGG 2 cut(s) 58, 735
BstSFI CTRYAG 2 cut(s) 347, 519
BstUI CGCG 2 cut(s) 798, 1173
BstV1I GCAGC 6 cut(s) 20, 333, 361, 505, 533, 838
BstV2I GAAGAC 3 cut(s) 216, 726, 815
BstZI CGGCCG 1 cut(s) 218
BsuRI GGCC 4 cut(s) 220, 874, 1188, 1249
BtgI CCRYGG 1 cut(s) 869
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 3 cut(s) 369, 567, 1171
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 909, 1087
CseI GACGC 1 cut(s) 813
Csp6I GTAC 1 cut(s) 626
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
CviAII CATG 7 cut(s) 312, 329, 368, 390, 566, 1091, 1139
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 3 cut(s) 303, 1115, 1278
DpnI GATC 2 cut(s) 816, 978
DpnII GATC 2 cut(s) 814, 976
DraI TTTAAA 2 cut(s) 99, 265
DrdI GACNNNNNNGTC 2 cut(s) 728, 1234
DseDI GACNNNNNNGTC 2 cut(s) 728, 1234
EaeI YGGCCR 2 cut(s) 218, 872
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 2 cut(s) 649, 1051
EarI CTCTTC 2 cut(s) 649, 1051
EclXI CGGCCG 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 1203
Eco47I GGWCC 2 cut(s) 909, 1087
Eco52I CGGCCG 1 cut(s) 218
Eco57I CTGAAG 3 cut(s) 32, 201, 996
EcoRII CCWGG 1 cut(s) 735
EcoT14I CCWWGG 1 cut(s) 1203
ErhI CCWWGG 1 cut(s) 1203
FaeI CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 3 cut(s) 851, 1137, 1222
FatI CATG 7 cut(s) 311, 328, 367, 389, 565, 1090, 1138
FbaI TGATCA 1 cut(s) 976
Fnu4HI GCNGC 7 cut(s) 34, 347, 350, 519, 522, 852, 1174
Fsp4HI GCNGC 7 cut(s) 34, 347, 350, 519, 522, 852, 1174
FspBI CTAG 2 cut(s) 900, 1071
GlaI GCGC 1 cut(s) 797
GluI GCNGC 7 cut(s) 34, 347, 350, 519, 522, 852, 1174
GsaI CCCAGC 1 cut(s) 88
HaeIII GGCC 4 cut(s) 220, 874, 1188, 1249
HapII CCGG 5 cut(s) 60, 221, 461, 907, 1265
HgaI GACGC 1 cut(s) 813
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 805
Hin1II CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HinfI GANTC 7 cut(s) 5, 11, 840, 1042, 1150, 1228, 1235
HpaII CCGG 5 cut(s) 60, 221, 461, 907, 1265
HphI GGTGA 3 cut(s) 611, 1040, 1097
Hpy166II GTNNAC 4 cut(s) 157, 619, 733, 896
Hpy188I TCNGA 7 cut(s) 10, 51, 491, 918, 973, 1015, 1041
Hpy188III TCNNGA 4 cut(s) 73, 131, 383, 1232
Hpy8I GTNNAC 4 cut(s) 157, 619, 733, 896
HpyAV CCTTC 2 cut(s) 128, 164
HpyCH4III ACNGT 4 cut(s) 498, 535, 725, 1052
HpyCH4IV ACGT 1 cut(s) 628
HpyF10VI GCNNNNNNNGC 6 cut(s) 377, 421, 518, 757, 804, 1166
HpyF3I CTNAG 3 cut(s) 303, 1115, 1278
HpySE526I ACGT 1 cut(s) 628
Hsp92I GRCGYC 1 cut(s) 805
Hsp92II CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
HspAI GCGC 1 cut(s) 796
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 2 cut(s) 814, 976
LmnI GCTCC 3 cut(s) 68, 421, 780
Lsp1109I GCAGC 6 cut(s) 20, 333, 361, 505, 533, 838
LweI GCATC 1 cut(s) 652
MaeI CTAG 2 cut(s) 900, 1071
MaeII ACGT 1 cut(s) 628
MaeIII GTNAC 4 cut(s) 184, 1131, 1180, 1270
MalI GATC 2 cut(s) 816, 978
MboI GATC 2 cut(s) 814, 976
MboII GAAGA 7 cut(s) 221, 666, 728, 731, 800, 815, 1068
MfeI CAATTG 2 cut(s) 426, 876
MlsI TGGCCA 1 cut(s) 874
MluCI AATT 6 cut(s) 93, 291, 426, 603, 876, 1251
MluNI TGGCCA 1 cut(s) 874
MlyI GAGTC 3 cut(s) 1159, 1222, 1244
MmeI TCCRAC 1 cut(s) 484
Mox20I TGGCCA 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 967
MscI TGGCCA 1 cut(s) 874
MseI TTAA 3 cut(s) 98, 198, 264
MslI CAYNNNNRTG 1 cut(s) 993
Msp20I TGGCCA 1 cut(s) 874
MspI CCGG 5 cut(s) 60, 221, 461, 907, 1265
MspR9I CCNGG 2 cut(s) 60, 737
MunI CAATTG 2 cut(s) 426, 876
MvaI CCWGG 1 cut(s) 737
MvnI CGCG 2 cut(s) 798, 1173
MwoI GCNNNNNNNGC 6 cut(s) 377, 421, 518, 757, 804, 1166
NciI CCSGG 1 cut(s) 60
NdeII GATC 2 cut(s) 814, 976
NlaIII CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
NlaIV GGNNCC 3 cut(s) 64, 423, 468
NmuCI GTSAC 2 cut(s) 1180, 1270
NspI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
PaeI GCATGC 2 cut(s) 371, 569
PciI ACATGT 1 cut(s) 1138
PdmI GAANNNNTTC 1 cut(s) 967
PfeI GAWTC 3 cut(s) 11, 840, 1042
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 7 cut(s) 35, 348, 351, 520, 523, 853, 1175
PleI GAGTC 3 cut(s) 1158, 1222, 1243
PpsI GAGTC 3 cut(s) 1158, 1222, 1243
Ppu21I YACGTR 1 cut(s) 629
PscI ACATGT 1 cut(s) 1138
Psp6I CCWGG 1 cut(s) 735
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 3 cut(s) 64, 423, 468
PspPI GGNCC 2 cut(s) 909, 1087
PstI CTGCAG 2 cut(s) 351, 523
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 993
SaqAI TTAA 3 cut(s) 98, 198, 264
SatI GCNGC 7 cut(s) 34, 347, 350, 519, 522, 852, 1174
Sau3AI GATC 2 cut(s) 814, 976
Sau96I GGNCC 2 cut(s) 909, 1087
SchI GAGTC 3 cut(s) 1159, 1222, 1244
ScrFI CCNGG 2 cut(s) 60, 737
SfaNI GCATC 1 cut(s) 652
SfcI CTRYAG 2 cut(s) 347, 519
SinI GGWCC 2 cut(s) 909, 1087
SmiMI CAYNNNNRTG 1 cut(s) 993
SmlI CTYRAG 3 cut(s) 71, 383, 1230
SmoI CTYRAG 3 cut(s) 71, 383, 1230
SpeI ACTAGT 1 cut(s) 1070
SphI GCATGC 2 cut(s) 371, 569
Sse9I AATT 6 cut(s) 93, 291, 426, 603, 876, 1251
SsiI CCGC 2 cut(s) 1108, 1173
SspMI CTAG 2 cut(s) 900, 1071
StyD4I CCNGG 2 cut(s) 58, 735
StyI CCWWGG 1 cut(s) 1203
TaaI ACNGT 4 cut(s) 498, 535, 725, 1052
TaiI ACGT 1 cut(s) 631
TaqI TCGA 2 cut(s) 652, 838
TasI AATT 6 cut(s) 93, 291, 426, 603, 876, 1251
TauI GCSGC 1 cut(s) 1176
TfiI GAWTC 3 cut(s) 11, 840, 1042
Tru1I TTAA 3 cut(s) 98, 198, 264
Tru9I TTAA 3 cut(s) 98, 198, 264
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 2 cut(s) 1180, 1270
TseI GCWGC 6 cut(s) 33, 346, 349, 518, 521, 851
Tsp45I GTSAC 2 cut(s) 1180, 1270
TspDTI ATGAA 4 cut(s) 151, 222, 288, 345
TspRI CASTG 1 cut(s) 21
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 909, 1087
XceI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 967
XspI CTAG 2 cut(s) 900, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.