RLG00000028176

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009803
Physical Location & Seq
Forward (+)
19752565 .. 19761809
9245 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028176

Sequence Viewer

Length: 2400 bp
ATGGACTCCGAATCAGTGATTGAGTTTCTGGGCTGCGTTCCTCTGCTTCAGCGCCTACCCAGCTCCTCGCTCAAGAAAATCGCCCAGCTTGTAATTTTAAAGCACTACGAGAAAGGAGAGTATGTTGTTCGTGAAGGGGAGGTTGGGATTGGGGTTTACTTCATTTGGGAAGGAGAGGCTGAAGTTACTGGGTCTGTTAATGCTGATGAAGACAATCGGCCGGAGTTTCAGTTGAAAAGATATGATTACTTTGGTTATGGTTTTAAAGCATTAGGTCATCAAGCTGATGTAATTGCTTCATCTAAGCTGACATGCTTGGTGCTACCCCATGAACATTATTCTCTGCTGCAGCCAAAATCAATCTGGCATGCTGATAAAGCTCTTGAGACATGCTCTCTTGTTGAGAATATATTGCATTTGGAGCCAATTGAAGCAAATATATTTCAGGGAATTACTTTACCGGAGGCACCAAGATTTGGACAGGTTTTCGGAGGACAGTTAGTTGGACAGGCATTGGCTGCAGCAACAAAAACTGTTGATTGTCTCAAAGTCGTCCATAGTTTGCATGCACATTTTTTGCTTGTCGGGGATTTCAACACACCAATTATATATCAAGTTCACCGAGTACGTGATGGAAAGAGCTTTGCTACTCGAAGAGTAGATGCAATACAAAAAGGGATAAACATATTCACTTTACTGGCTTCCTTTCAAAAAGAAGAAGAAGGGTCTGTCCACCAGGAAGCCATAATGCCATCTGTGCCTCCCCCAGATACGCTCCCATCAATGGAAGAATTGCGAGAAAGACGCCTTTTTGATCCCCGTCTTCCAAGAACTTATCGAATCAAGGTAGCTGCAAAGGAGTTTGTCCCGTGGCCAATTGAGATACGATTTTGTGAACCTAGTAGCCGGACCAATCAGACTAAATCTCCACCAAGATTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCAGATGATCAGGCTTTGCATAGATGTGTTGTGGCTTATGCTTCAGATTTGATATTCGCTGGGGTAAGTCTGAATCCTCACCGTAGAAGAGGACATAAAACAAGTCAAGTTAGTTTGGACCATGCAATGTGGTTTCACCGCCCCCTTAGAGCTGATGACTGGTTACTACATGTGATAGTGAGTCCCATTGCCTATAACGCACGTGGCTATGTCACAGGCCAAATGTTCAATACCAAGGGAGAGCTTGTTGTGTCATTGACTCAAGAGTCCCTGAATAGGCCAATTAGACCAACTCCGGCTGTCACATCGAACCAACAGCTTCTCAGGAAATTCACTTACTGTAACATTAGAATGGAACAGCCTGGTAATGGAACAGATGGGATCAGTCAAATGGAACCGGCCCAACAGCCACTGATATCTGGACTTCCTGATGACATTGCTCTTTTCTGCATGGCAAGGGTTCCCCGAAAGAACCATACAGTCCTTAAGTGTGTTTCAAAAAGATGGCAACACTTAGTGGGCAGTGAAGAGTGGCATTCCTACCGTCAAAAGCATAATCTTGATGAGACTTGGATCTATGCTTTGTGCCGAGACAAATTGGAGCGTCTTTGCCTGTATGTATTGGATCCAAAGTCATCAAGAAGGAGTTGGAAGCTTGTTAATGAGCTTCCTCCTCGCACCTTGAAGAGAAAAGGCATGGGTTTTGAAGTGCTAGGAAAGAAAGTTTACTTGTTGGGTGGTTGCGGTTGGTGTGAAGATGCTACAGATGAGGTCTACTACTATGATGCTTCCCTGAACACTTGGAGTGAAGCTGCTCCCTTATCTATTGCTAGGTGCTATTTTGCCTGTGAAGTATTAGATGAGAAAATGTATTCCATTGGTGGATTAGGATCAAATTCAAGTGATCCACATTCTTGGGACATTTATGATCCTAGCACTAATAGTTGGATATTTCACTCAGACCCAAACATTGTCCCTGAAATTGAGGATTCTGTGGTCATGGATGGGAGAATCTACATCCGCTGTGGCACTTCTGCTGTAACTTCTCATGTCTATGCTGTTGTTTATGAACCATCAAGCGGTACATGGCAGCACGCAGATGCTGATATGGTCTCTGGCTGGCGAGGTCCGGCAGTTGTAATAGACGAGACCCTCTATGTATTGGATCAGAGCTCAGGAACTAGGCTGATGATGTGGCAGAAGGAGACTAGGGAATGGATTCCAGTTGGGAGGCTGTCATCCTTGCTTACCAGACCACCCTGTCAACTCGTAGCTATTGGGAAGAGTATTTATGTTGTCGGAAAGGGGCTTAGCACAGTCATGTTTGATGTTGAAAATGCAGGGAATGTGGAAGGGGTAATTGTGAGTTCTTCAATACCTAAATTGAATTCTGATGATGATGTAATCAGCTGTAAATGTTTGTCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

800

Amino Acids

90.1

Weight (kDa)

6.69

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 34 - 112 7.7e-08 Cyclic nucleotide-binding domain
4HBT_3 PF13622 158 - 237 2.1e-17 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 302 - 413 5.7e-23 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 307 - 412 1.7e-23 Acyl-CoA thioesterase
F-box PF00646 462 - 500 1.8e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 528 - 690 8.7e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 533 - 742 2.7e-25 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 536 - 710 8.4e-08 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 553 - 597 3.5e-07 Kelch motif
Beta-prop_Calicin PF13964 553 - 715 1.4e-08 Calicin, beta-propeller domain
Kelch_1 PF01344 600 - 639 5.9e-06 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1234, 2229
AccB1I GGYRCC 1 cut(s) 466
AccB7I CCANNNNNTGG 1 cut(s) 476
AccI GTMKAC 1 cut(s) 1743
AciI CCGC 4 cut(s) 1108, 1713, 1990, 2049
AclWI GGATC 9 cut(s) 809, 1358, 1550, 1589, 1602, 1867, 1868, 1892, 2142
AcoI YGGCCR 2 cut(s) 218, 872
AcsI RAATTY 3 cut(s) 1298, 1864, 2356
AcuI CTGAAG 3 cut(s) 32, 201, 996
AcvI CACGTG 1 cut(s) 1172
AcyI GRCGYC 1 cut(s) 805
AdeI CACNNNGTG 1 cut(s) 1486
AfaI GTAC 2 cut(s) 627, 2053
AfiI CCNNNNNNNGG 5 cut(s) 476, 784, 1245, 1337, 2048
AflII CTTAAG 1 cut(s) 1454
AflIII ACRYGT 1 cut(s) 1138
AjnI CCWGG 2 cut(s) 735, 1330
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
Alw21I GWGCWC 1 cut(s) 2144
Alw26I GTCTC 7 cut(s) 380, 548, 1529, 1554, 2086, 2111, 2168
AlwI GGATC 9 cut(s) 809, 1358, 1550, 1589, 1602, 1867, 1868, 1892, 2142
AlwNI CAGNNNCTG 2 cut(s) 1381, 2072
AoxI GGCC 5 cut(s) 218, 872, 1186, 1247, 1368
ApeKI GCWGC 8 cut(s) 33, 346, 349, 518, 521, 851, 1781, 2059
ApoI RAATTY 3 cut(s) 1298, 1864, 2356
Asp700I GAANNNNTTC 2 cut(s) 967, 2187
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 4 cut(s) 909, 1087, 1369, 2096
AsuHPI GGTGA 3 cut(s) 611, 1040, 1097
AvaII GGWCC 3 cut(s) 909, 1087, 2096
BalI TGGCCA 1 cut(s) 874
BamHI GGATCC 1 cut(s) 1594
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 2144
BbrPI CACGTG 1 cut(s) 1172
BbsI GAAGAC 2 cut(s) 216, 815
Bbv12I GWGCWC 1 cut(s) 2144
BbvI GCAGC 8 cut(s) 20, 333, 361, 505, 533, 838, 1768, 2071
BccI CCATC 7 cut(s) 626, 760, 787, 1340, 1467, 1967, 2050
BciT130I CCWGG 2 cut(s) 737, 1332
BclI TGATCA 1 cut(s) 976
BcoDI GTCTC 7 cut(s) 380, 548, 1529, 1554, 2086, 2111, 2168
BfaI CTAG 6 cut(s) 900, 1682, 1800, 1902, 2151, 2178
BfmI CTRYAG 3 cut(s) 347, 519, 1731
BfoI RGCGCY 1 cut(s) 55
BfrI CTTAAG 1 cut(s) 1454
BisI GCNGC 8 cut(s) 34, 347, 350, 519, 522, 852, 1782, 2060
BlpI GCTNAGC 1 cut(s) 2279
BlsI GCNGC 8 cut(s) 35, 348, 351, 520, 523, 853, 1783, 2061
Bme1390I CCNGG 2 cut(s) 737, 1332
Bme18I GGWCC 3 cut(s) 909, 1087, 2096
BmgT120I GGNCC 4 cut(s) 909, 1087, 1369, 2096
BmiI GGNNCC 5 cut(s) 423, 468, 1365, 1431, 1596
BmrFI CCNGG 2 cut(s) 737, 1332
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 4 cut(s) 652, 1717, 1744, 2059
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 216, 815
BplI GAGNNNNNCTC 2 cut(s) 377, 409
Bpu10I CCTNAGC 1 cut(s) 2143
Bpu1102I GCTNAGC 1 cut(s) 2279
BpuEI CTTGAG 3 cut(s) 56, 404, 1215
BsaAI YACGTR 2 cut(s) 629, 1172
BsaBI GATNNNNATC 1 cut(s) 1858
BsaHI GRCGYC 1 cut(s) 805
BsaI GGTCTC 2 cut(s) 2086, 2111
BsaJI CCNNGG 2 cut(s) 869, 1203
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 8 cut(s) 108, 138, 215, 245, 910, 940, 1200, 1230
Bsc4I CCNNNNNNNGG 5 cut(s) 476, 784, 1245, 1337, 2048
Bse118I RCCGGY 1 cut(s) 1366
Bse1I ACTGG 4 cut(s) 193, 702, 1133, 2192
Bse3DI GCAATG 3 cut(s) 1101, 1155, 1404
Bse8I GATNNNNATC 1 cut(s) 1858
BseBI CCWGG 2 cut(s) 737, 1332
BseDI CCNNGG 2 cut(s) 869, 1203
BseGI GGATG 3 cut(s) 1978, 1986, 2207
BseJI GATNNNNATC 1 cut(s) 1858
BseLI CCNNNNNNNGG 5 cut(s) 476, 784, 1245, 1337, 2048
BseMI GCAATG 3 cut(s) 1101, 1155, 1404
BseMII CTCAG 3 cut(s) 1306, 1941, 2157
BseNI ACTGG 4 cut(s) 193, 702, 1133, 2192
BseRI GAGGAG 2 cut(s) 55, 1632
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 8 cut(s) 20, 333, 361, 505, 533, 838, 1768, 2071
BseYI CCCAGC 3 cut(s) 59, 84, 1028
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 5 cut(s) 220, 874, 1188, 1249, 1370
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 2144
BsiSI CCGG 6 cut(s) 221, 461, 907, 1265, 1367, 2099
BslFI GGGAC 5 cut(s) 851, 1137, 1222, 1901, 1928
BslI CCNNNNNNNGG 5 cut(s) 476, 784, 1245, 1337, 2048
BsmAI GTCTC 7 cut(s) 380, 548, 1529, 1554, 2086, 2111, 2168
BsmFI GGGAC 5 cut(s) 851, 1137, 1222, 1901, 1928
BsmI GAATGC 1 cut(s) 1504
BsnI GGCC 5 cut(s) 220, 874, 1188, 1249, 1370
Bso31I GGTCTC 2 cut(s) 2086, 2111
Bsp1286I GDGCHC 1 cut(s) 2144
Bsp143I GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
Bsp1720I GCTNAGC 1 cut(s) 2279
BspACI CCGC 4 cut(s) 1108, 1713, 1990, 2049
BspANI GGCC 5 cut(s) 220, 874, 1188, 1249, 1370
BspCNI CTCAG 3 cut(s) 1305, 1940, 2156
BspLI GGNNCC 5 cut(s) 423, 468, 1365, 1431, 1596
BspMAI CTGCAG 2 cut(s) 351, 523
BspPI GGATC 9 cut(s) 809, 1358, 1550, 1589, 1602, 1867, 1868, 1892, 2142
BspT107I GGYRCC 1 cut(s) 466
BspTI CTTAAG 1 cut(s) 1454
BspTNI GGTCTC 2 cut(s) 2086, 2111
BsrDI GCAATG 3 cut(s) 1101, 1155, 1404
BsrFI RCCGGY 1 cut(s) 1366
BsrI ACTGG 4 cut(s) 193, 702, 1133, 2192
BssAI RCCGGY 1 cut(s) 1366
BssECI CCNNGG 2 cut(s) 869, 1203
BssMI GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
BssNI GRCGYC 1 cut(s) 805
BssT1I CCWWGG 1 cut(s) 1203
Bst2UI CCWGG 2 cut(s) 737, 1332
Bst4CI ACNGT 7 cut(s) 498, 535, 1052, 1310, 1450, 1514, 2287
Bst6I CTCTTC 5 cut(s) 649, 1051, 1491, 1649, 2246
BstACI GRCGYC 1 cut(s) 805
BstAFI CTTAAG 1 cut(s) 1454
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 2 cut(s) 629, 1172
BstC8I GCNNGC 4 cut(s) 369, 567, 2064, 2090
BstDEI CTNAG 7 cut(s) 303, 1115, 1292, 1483, 1927, 2143, 2279
BstDSI CCRYGG 1 cut(s) 869
BstF5I GGATG 3 cut(s) 1978, 1986, 2207
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 9 cut(s) 817, 979, 1353, 1545, 1597, 1862, 1876, 1900, 2137
BstMAI GTCTC 7 cut(s) 380, 548, 1529, 1554, 2086, 2111, 2168
BstMBI GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 6 cut(s) 60, 377, 421, 518, 757, 1166
BstNI CCWGG 2 cut(s) 737, 1332
BstNSI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
BstSCI CCNGG 2 cut(s) 735, 1330
BstSFI CTRYAG 3 cut(s) 347, 519, 1731
BstV1I GCAGC 8 cut(s) 20, 333, 361, 505, 533, 838, 1768, 2071
BstV2I GAAGAC 2 cut(s) 216, 815
BstX2I RGATCY 2 cut(s) 1542, 1594
BstXI CCANNNNNNTGG 1 cut(s) 1884
BstYI RGATCY 2 cut(s) 1542, 1594
BstZI CGGCCG 1 cut(s) 218
BsuRI GGCC 5 cut(s) 220, 874, 1188, 1249, 1370
BtgI CCRYGG 1 cut(s) 869
BtsCI GGATG 3 cut(s) 1978, 1986, 2207
BtsI GCAGTG 1 cut(s) 1498
BtsIMutI CAGTG 3 cut(s) 21, 1379, 1498
Cac8I GCNNGC 4 cut(s) 369, 567, 2064, 2090
CaiI CAGNNNCTG 2 cut(s) 1381, 2072
CfoI GCGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 1366
Cfr13I GGNCC 4 cut(s) 909, 1087, 1369, 2096
CseI GACGC 2 cut(s) 813, 1562
Csp6I GTAC 2 cut(s) 626, 2052
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
CviQI GTAC 2 cut(s) 626, 2052
DdeI CTNAG 7 cut(s) 303, 1115, 1292, 1483, 1927, 2143, 2279
DpnI GATC 9 cut(s) 816, 978, 1352, 1544, 1596, 1861, 1875, 1899, 2136
DpnII GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
DraI TTTAAA 2 cut(s) 99, 265
DraIII CACNNNGTG 1 cut(s) 1486
DrdI GACNNNNNNGTC 2 cut(s) 1234, 2229
DseDI GACNNNNNNGTC 2 cut(s) 1234, 2229
EaeI YGGCCR 2 cut(s) 218, 872
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 5 cut(s) 649, 1051, 1491, 1649, 2246
EarI CTCTTC 5 cut(s) 649, 1051, 1491, 1649, 2246
Ecl136II GAGCTC 1 cut(s) 2142
EclXI CGGCCG 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 1203
Eco24I GRGCYC 1 cut(s) 2144
Eco31I GGTCTC 2 cut(s) 2086, 2111
Eco32I GATATC 1 cut(s) 1386
Eco47I GGWCC 3 cut(s) 909, 1087, 2096
Eco52I CGGCCG 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 2142
Eco57I CTGAAG 3 cut(s) 32, 201, 996
Eco72I CACGTG 1 cut(s) 1172
EcoICRI GAGCTC 1 cut(s) 2142
EcoRI GAATTC 1 cut(s) 2356
EcoRII CCWGG 2 cut(s) 735, 1330
EcoRV GATATC 1 cut(s) 1386
EcoT14I CCWWGG 1 cut(s) 1203
EcoT38I GRGCYC 1 cut(s) 2144
ErhI CCWWGG 1 cut(s) 1203
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 5 cut(s) 851, 1137, 1222, 1901, 1928
FbaI TGATCA 1 cut(s) 976
FblI GTMKAC 1 cut(s) 1743
Fnu4HI GCNGC 8 cut(s) 34, 347, 350, 519, 522, 852, 1782, 2060
FokI GGATG 3 cut(s) 1973, 1985, 2194
FriOI GRGCYC 1 cut(s) 2144
Fsp4HI GCNGC 8 cut(s) 34, 347, 350, 519, 522, 852, 1782, 2060
FspBI CTAG 6 cut(s) 900, 1682, 1800, 1902, 2151, 2178
GlaI GCGC 1 cut(s) 53
GluI GCNGC 8 cut(s) 34, 347, 350, 519, 522, 852, 1782, 2060
GsaI CCCAGC 3 cut(s) 63, 88, 1032
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 5 cut(s) 220, 874, 1188, 1249, 1370
HapII CCGG 6 cut(s) 221, 461, 907, 1265, 1367, 2099
HgaI GACGC 2 cut(s) 813, 1562
HhaI GCGC 1 cut(s) 54
Hin1I GRCGYC 1 cut(s) 805
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HincII GTYRAC 1 cut(s) 2234
HindII GTYRAC 1 cut(s) 2234
HindIII AAGCTT 1 cut(s) 1622
HpaII CCGG 6 cut(s) 221, 461, 907, 1265, 1367, 2099
HphI GGTGA 3 cut(s) 611, 1040, 1097
Hpy166II GTNNAC 7 cut(s) 157, 619, 733, 896, 1696, 1744, 2234
Hpy8I GTNNAC 7 cut(s) 157, 619, 733, 896, 1696, 1744, 2234
HpyAV CCTTC 6 cut(s) 128, 164, 716, 1605, 2164, 2315
HpyCH4III ACNGT 7 cut(s) 498, 535, 1052, 1310, 1450, 1514, 2287
HpyCH4IV ACGT 2 cut(s) 628, 1171
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 377, 421, 518, 757, 1166
HpyF3I CTNAG 7 cut(s) 303, 1115, 1292, 1483, 1927, 2143, 2279
HpySE526I ACGT 2 cut(s) 628, 1171
Hsp92I GRCGYC 1 cut(s) 805
HspAI GCGC 1 cut(s) 52
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
LmnI GCTCC 5 cut(s) 68, 421, 780, 1570, 1789
Lsp1109I GCAGC 8 cut(s) 20, 333, 361, 505, 533, 838, 1768, 2071
LweI GCATC 4 cut(s) 652, 1717, 1744, 2059
MaeI CTAG 6 cut(s) 900, 1682, 1800, 1902, 2151, 2178
MaeII ACGT 2 cut(s) 628, 1171
MaeIII GTNAC 6 cut(s) 184, 1131, 1180, 1270, 1310, 2008
MalI GATC 9 cut(s) 816, 978, 1352, 1544, 1596, 1861, 1875, 1899, 2136
MboI GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
MfeI CAATTG 2 cut(s) 426, 876
MflI RGATCY 2 cut(s) 1542, 1594
MhlI GDGCHC 1 cut(s) 2144
MlsI TGGCCA 1 cut(s) 874
MluNI TGGCCA 1 cut(s) 874
MlyI GAGTC 3 cut(s) 1159, 1222, 1244
MmeI TCCRAC 4 cut(s) 484, 1598, 1895, 2248
Mox20I TGGCCA 1 cut(s) 874
MroXI GAANNNNTTC 2 cut(s) 967, 2187
MscI TGGCCA 1 cut(s) 874
MseI TTAA 5 cut(s) 98, 198, 264, 1455, 1629
MslI CAYNNNNRTG 5 cut(s) 993, 1319, 2022, 2067, 2288
Msp20I TGGCCA 1 cut(s) 874
MspA1I CMGCKG 2 cut(s) 1992, 2379
MspCI CTTAAG 1 cut(s) 1454
MspI CCGG 6 cut(s) 221, 461, 907, 1265, 1367, 2099
MspR9I CCNGG 2 cut(s) 737, 1332
MunI CAATTG 2 cut(s) 426, 876
Mva1269I GAATGC 1 cut(s) 1504
MvaI CCWGG 2 cut(s) 737, 1332
MwoI GCNNNNNNNGC 6 cut(s) 60, 377, 421, 518, 757, 1166
NdeII GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
NlaIV GGNNCC 5 cut(s) 423, 468, 1365, 1431, 1596
NmeAIII GCCGAG 1 cut(s) 1583
NmuCI GTSAC 2 cut(s) 1180, 1270
NspI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
PaeI GCATGC 2 cut(s) 371, 569
PciI ACATGT 1 cut(s) 1138
PctI GAATGC 1 cut(s) 1504
PdmI GAANNNNTTC 2 cut(s) 967, 2187
PfeI GAWTC 6 cut(s) 11, 840, 1042, 1958, 1980, 2188
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 8 cut(s) 35, 348, 351, 520, 523, 853, 1783, 2061
PleI GAGTC 3 cut(s) 1158, 1222, 1243
PmaCI CACGTG 1 cut(s) 1172
PmlI CACGTG 1 cut(s) 1172
PpsI GAGTC 3 cut(s) 1158, 1222, 1243
Ppu21I YACGTR 2 cut(s) 629, 1172
PscI ACATGT 1 cut(s) 1138
Psp124BI GAGCTC 1 cut(s) 2144
Psp6I CCWGG 2 cut(s) 735, 1330
PspCI CACGTG 1 cut(s) 1172
PspFI CCCAGC 3 cut(s) 59, 84, 1028
PspGI CCWGG 2 cut(s) 735, 1330
PspN4I GGNNCC 5 cut(s) 423, 468, 1365, 1431, 1596
PspPI GGNCC 4 cut(s) 909, 1087, 1369, 2096
PstI CTGCAG 2 cut(s) 351, 523
PstNI CAGNNNCTG 2 cut(s) 1381, 2072
PsuI RGATCY 2 cut(s) 1542, 1594
PvuII CAGCTG 1 cut(s) 2379
RsaI GTAC 2 cut(s) 627, 2053
RsaNI GTAC 2 cut(s) 626, 2052
RseI CAYNNNNRTG 5 cut(s) 993, 1319, 2022, 2067, 2288
SacI GAGCTC 1 cut(s) 2144
SaqAI TTAA 5 cut(s) 98, 198, 264, 1455, 1629
SatI GCNGC 8 cut(s) 34, 347, 350, 519, 522, 852, 1782, 2060
Sau3AI GATC 9 cut(s) 814, 976, 1350, 1542, 1594, 1859, 1873, 1897, 2134
Sau96I GGNCC 4 cut(s) 909, 1087, 1369, 2096
SchI GAGTC 3 cut(s) 1159, 1222, 1244
ScrFI CCNGG 2 cut(s) 737, 1332
SduI GDGCHC 1 cut(s) 2144
SfaNI GCATC 4 cut(s) 652, 1717, 1744, 2059
SfcI CTRYAG 3 cut(s) 347, 519, 1731
SinI GGWCC 3 cut(s) 909, 1087, 2096
SmiMI CAYNNNNRTG 5 cut(s) 993, 1319, 2022, 2067, 2288
SmlI CTYRAG 4 cut(s) 71, 383, 1230, 1454
SmoI CTYRAG 4 cut(s) 71, 383, 1230, 1454
SphI GCATGC 2 cut(s) 371, 569
SsiI CCGC 4 cut(s) 1108, 1713, 1990, 2049
SspMI CTAG 6 cut(s) 900, 1682, 1800, 1902, 2151, 2178
SstI GAGCTC 1 cut(s) 2144
StyD4I CCNGG 2 cut(s) 735, 1330
StyI CCWWGG 1 cut(s) 1203
TaaI ACNGT 7 cut(s) 498, 535, 1052, 1310, 1450, 1514, 2287
TaiI ACGT 2 cut(s) 631, 1174
TaqI TCGA 3 cut(s) 652, 838, 1277
TfiI GAWTC 6 cut(s) 11, 840, 1042, 1958, 1980, 2188
Tru1I TTAA 5 cut(s) 98, 198, 264, 1455, 1629
Tru9I TTAA 5 cut(s) 98, 198, 264, 1455, 1629
TscAI CASTG 3 cut(s) 21, 1386, 1498
TseFI GTSAC 2 cut(s) 1180, 1270
TseI GCWGC 8 cut(s) 33, 346, 349, 518, 521, 851, 1781, 2059
Tsp45I GTSAC 2 cut(s) 1180, 1270
TspDTI ATGAA 5 cut(s) 151, 222, 288, 345, 2052
TspRI CASTG 3 cut(s) 21, 1386, 1498
Van91I CCANNNNNTGG 1 cut(s) 476
Vha464I CTTAAG 1 cut(s) 1454
VpaK11BI GGWCC 3 cut(s) 909, 1087, 2096
XapI RAATTY 3 cut(s) 1298, 1864, 2356
XceI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmiI GTMKAC 1 cut(s) 1743
XmnI GAANNNNTTC 2 cut(s) 967, 2187
XspI CTAG 6 cut(s) 900, 1682, 1800, 1902, 2151, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.