pycom01g08780

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009803
Physical Location & Seq
Reverse (-)
9722495 .. 9723869
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g08780.2

Sequence Viewer

Length: 1080 bp
ATGGAACTGCCAGGTGATGGATCAGATGTGATTAGTCAAACGGAACTGACCCAACAGCCACTTTTATGTGGACTTCCAGATGATATTGCTCTTTTCTGCCTATCAAGGGTGCCTCGGAAGTACCATACCCTCTTAAAGTGTGTCTCAAAAAGATGGAGAGACTTGGTGTGTAGTGAAGAGTGGCATTCTTACCGTAGAAAGCATAAACTTGATGAGACTTGGATCTATGCTTTGTGCCGAGACAAGTTGGACCGCCTTTGCTTTTATGTGTTGGATCCAAACTCATCAAGGCGGTCTTGGAAGCTTGTTCATGGGCTTCCGCCTCGTATCATGAAGAGAAAGGGAATGGGTTTTGAAGTTCTAGGAAAGAAAGTTTACTTATTGGGTGGTTGCGGTTGGTGCGAAGATGCTACTGATGAGGTCTACTGTTATGATGCTTCCCTGAATGCTTGGAGTGAAGCTTCTCCCTTGTCCACTGCTAGGTGCTACTTTGCTTGTGAAATATTGGATGACAAAATATATTCCATTGGCGGGTTGGGTTCAATTTCAAGCTATCCGCATTCTTGGGACATATATGACCCTTGCACTGATAGTTGGAAATTTCACTCAGACCCAAACTTTGTTCCTGAAATTGAGGATTCTGTGGTCATGGCCGGGAAGATTTACATTCGTTGTGGCACTTCTGCTGTAACTTCTCATATGCACGCTGTTGTTTATGAACCGTCAAGTGGGACATGGCAGCACGCAGATTCTGAAATGGTTTCAGGCTGGCGAGGTCCAGCAGTTGTCATAGACGGAACCCTCTATGTGTTGGATCAGAGTTCAGGAACGAGGCTGATGATGTGGGAGAAGGAAACTAGGGAGTGGATCCCTGTCGGGAGGCTGTCATCCTTGCTTACTAGACCGCCCTGTCAACTCGTAGCTATGGGGAAGAAATTTTATGTTGTGGGAAAGGGGCTTAGCACAGTCATGTTTGATGTTGAAAATGCAGGGAATATGGAAGGGATGATGGTTAGTTCTTCAATACCTAAATTGAATTCAGACATTGATGTAATCGGCTGTAAATGTTTATCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

360

Amino Acids

40.44

Weight (kDa)

6.01

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 909
AccB1I GGYRCC 1 cut(s) 109
AccB7I CCANNNNNTGG 1 cut(s) 17
AccI GTMKAC 1 cut(s) 423
AciI CCGC 7 cut(s) 253, 292, 320, 393, 531, 557, 905
AclWI GGATC 7 cut(s) 28, 230, 269, 282, 822, 862, 875
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 3 cut(s) 599, 935, 1036
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 5 cut(s) 17, 106, 480, 531, 728
AgsI TTSAA 6 cut(s) 356, 543, 549, 983, 1023, 1036
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 4 cut(s) 304, 461, 552, 923
AluI AGCT 4 cut(s) 304, 461, 552, 923
Alw26I GTCTC 4 cut(s) 148, 153, 209, 234
AlwI GGATC 7 cut(s) 28, 230, 269, 282, 822, 862, 875
AlwNI CAGNNNCTG 1 cut(s) 752
AoxI GGCC 1 cut(s) 651
ApeKI GCWGC 1 cut(s) 739
ApoI RAATTY 3 cut(s) 599, 935, 1036
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AspS9I GGNCC 2 cut(s) 250, 776
AsuC2I CCSGG 1 cut(s) 655
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 2 cut(s) 250, 776
BamHI GGATCC 2 cut(s) 274, 867
BanI GGYRCC 1 cut(s) 109
BbvI GCAGC 1 cut(s) 751
BccI CCATC 3 cut(s) 11, 147, 1003
BciT130I CCWGG 1 cut(s) 12
BcnI CCSGG 1 cut(s) 655
BcoDI GTCTC 4 cut(s) 148, 153, 209, 234
BfaI CTAG 4 cut(s) 362, 480, 858, 900
BisI GCNGC 1 cut(s) 740
BlpI GCTNAGC 1 cut(s) 959
BlsI GCNGC 1 cut(s) 741
Bme1390I CCNGG 2 cut(s) 12, 655
Bme18I GGWCC 2 cut(s) 250, 776
BmgT120I GGNCC 2 cut(s) 250, 776
BmiI GGNNCC 4 cut(s) 111, 276, 799, 869
BmrFI CCNGG 2 cut(s) 12, 655
BmsI GCATC 2 cut(s) 397, 424
Bpu1102I GCTNAGC 1 cut(s) 959
BpuMI CCSGG 1 cut(s) 655
Bsa29I ATCGAT 1 cut(s) 1073
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 106, 480, 531, 728
BseBI CCWGG 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 1073
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 3 cut(s) 514, 887, 1011
BseLI CCNNNNNNNGG 5 cut(s) 17, 106, 480, 531, 728
BseMII CTCAG 1 cut(s) 621
BseXI GCAGC 1 cut(s) 751
BshFI GGCC 1 cut(s) 653
BshNI GGYRCC 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 1073
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 2 cut(s) 581, 745
BslI CCNNNNNNNGG 5 cut(s) 17, 106, 480, 531, 728
BsmAI GTCTC 4 cut(s) 148, 153, 209, 234
BsmFI GGGAC 2 cut(s) 581, 745
BsmI GAATGC 3 cut(s) 184, 451, 559
BsnI GGCC 1 cut(s) 653
Bsp143I GATC 5 cut(s) 20, 222, 274, 814, 867
Bsp1720I GCTNAGC 1 cut(s) 959
BspACI CCGC 7 cut(s) 253, 292, 320, 393, 531, 557, 905
BspANI GGCC 1 cut(s) 653
BspCNI CTCAG 1 cut(s) 620
BspDI ATCGAT 1 cut(s) 1073
BspHI TCATGA 1 cut(s) 330
BspLI GGNNCC 4 cut(s) 111, 276, 799, 869
BspPI GGATC 7 cut(s) 28, 230, 269, 282, 822, 862, 875
BspT107I GGYRCC 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 5 cut(s) 20, 222, 274, 814, 867
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 4 cut(s) 194, 428, 723, 967
Bst6I CTCTTC 2 cut(s) 171, 329
BstC8I GCNNGC 3 cut(s) 705, 744, 770
BstDEI CTNAG 2 cut(s) 607, 959
BstF5I GGATG 3 cut(s) 514, 887, 1011
BstKTI GATC 5 cut(s) 23, 225, 277, 817, 870
BstMAI GTCTC 4 cut(s) 148, 153, 209, 234
BstMBI GATC 5 cut(s) 20, 222, 274, 814, 867
BstMWI GCNNNNNNNGC 1 cut(s) 399
BstNI CCWGG 1 cut(s) 12
BstSCI CCNGG 2 cut(s) 10, 653
BstV1I GCAGC 1 cut(s) 751
BstX2I RGATCY 3 cut(s) 222, 274, 867
BstYI RGATCY 3 cut(s) 222, 274, 867
Bsu15I ATCGAT 1 cut(s) 1073
BsuRI GGCC 1 cut(s) 653
BsuTUI ATCGAT 1 cut(s) 1073
BtsCI GGATG 3 cut(s) 514, 887, 1011
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 474, 585
Cac8I GCNNGC 3 cut(s) 705, 744, 770
CaiI CAGNNNCTG 1 cut(s) 752
CciI TCATGA 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 250, 776
ClaI ATCGAT 1 cut(s) 1073
Csp6I GTAC 1 cut(s) 121
CviAII CATG 5 cut(s) 311, 331, 649, 735, 970
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 2 cut(s) 607, 959
DpnI GATC 5 cut(s) 22, 224, 276, 816, 869
DpnII GATC 5 cut(s) 20, 222, 274, 814, 867
DrdI GACNNNNNNGTC 1 cut(s) 909
DseDI GACNNNNNNGTC 1 cut(s) 909
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 2 cut(s) 171, 329
EarI CTCTTC 2 cut(s) 171, 329
EciI GGCGGA 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 250, 776
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 1 cut(s) 10
FaeI CATG 5 cut(s) 314, 334, 652, 738, 973
FalI AAGNNNNNCTT 2 cut(s) 280, 312
FaqI GGGAC 2 cut(s) 581, 745
FatI CATG 5 cut(s) 310, 330, 648, 734, 969
FauI CCCGC 1 cut(s) 524
FauNDI CATATG 1 cut(s) 699
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 1 cut(s) 740
FokI GGATG 3 cut(s) 521, 874, 1018
Fsp4HI GCNGC 1 cut(s) 740
FspBI CTAG 4 cut(s) 362, 480, 858, 900
GluI GCNGC 1 cut(s) 740
HaeIII GGCC 1 cut(s) 653
HapII CCGG 1 cut(s) 654
Hin1II CATG 5 cut(s) 314, 334, 652, 738, 973
HincII GTYRAC 1 cut(s) 914
HindII GTYRAC 1 cut(s) 914
HindIII AAGCTT 2 cut(s) 302, 459
HinfI GANTC 2 cut(s) 638, 749
HpaII CCGG 1 cut(s) 654
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 5 cut(s) 71, 376, 424, 474, 914
Hpy188I TCNGA 6 cut(s) 25, 117, 610, 754, 819, 1042
Hpy188III TCNNGA 5 cut(s) 77, 331, 626, 825, 877
Hpy8I GTNNAC 5 cut(s) 71, 376, 424, 474, 914
HpyAV CCTTC 2 cut(s) 844, 995
HpyCH4III ACNGT 4 cut(s) 194, 428, 723, 967
HpyCH4V TGCA 3 cut(s) 585, 703, 989
HpyF10VI GCNNNNNNNGC 1 cut(s) 399
HpyF3I CTNAG 2 cut(s) 607, 959
Hsp92II CATG 5 cut(s) 314, 334, 652, 738, 973
Kzo9I GATC 5 cut(s) 20, 222, 274, 814, 867
Lsp1109I GCAGC 1 cut(s) 751
LweI GCATC 2 cut(s) 397, 424
MaeI CTAG 4 cut(s) 362, 480, 858, 900
MaeIII GTNAC 1 cut(s) 688
MalI GATC 5 cut(s) 22, 224, 276, 816, 869
MboI GATC 5 cut(s) 20, 222, 274, 814, 867
MboII GAAGA 6 cut(s) 188, 346, 416, 670, 943, 1011
MflI RGATCY 3 cut(s) 222, 274, 867
MluCI AATT 6 cut(s) 543, 599, 630, 935, 1031, 1036
MmeI TCCRAC 4 cut(s) 228, 252, 575, 792
MnlI CCTC 9 cut(s) 123, 140, 333, 412, 628, 767, 812, 825, 873
MseI TTAA 1 cut(s) 134
MslI CAYNNNNRTG 2 cut(s) 64, 968
MspI CCGG 1 cut(s) 654
MspR9I CCNGG 2 cut(s) 12, 655
Mva1269I GAATGC 3 cut(s) 184, 451, 559
MvaI CCWGG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 399
NciI CCSGG 1 cut(s) 655
NdeI CATATG 1 cut(s) 699
NdeII GATC 5 cut(s) 20, 222, 274, 814, 867
NlaIII CATG 5 cut(s) 314, 334, 652, 738, 973
NlaIV GGNNCC 4 cut(s) 111, 276, 799, 869
NmeAIII GCCGAG 1 cut(s) 263
PagI TCATGA 1 cut(s) 330
PctI GAATGC 3 cut(s) 184, 451, 559
PfeI GAWTC 2 cut(s) 638, 749
PflMI CCANNNNNTGG 1 cut(s) 17
PkrI GCNGC 1 cut(s) 741
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 4 cut(s) 111, 276, 799, 869
PspPI GGNCC 2 cut(s) 250, 776
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 3 cut(s) 222, 274, 867
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 2 cut(s) 64, 968
SaqAI TTAA 1 cut(s) 134
SatI GCNGC 1 cut(s) 740
Sau3AI GATC 5 cut(s) 20, 222, 274, 814, 867
Sau96I GGNCC 2 cut(s) 250, 776
ScrFI CCNGG 2 cut(s) 12, 655
SetI ASST 9 cut(s) 16, 306, 423, 463, 485, 554, 778, 925, 1030
SfaNI GCATC 2 cut(s) 397, 424
SinI GGWCC 2 cut(s) 250, 776
SmiMI CAYNNNNRTG 2 cut(s) 64, 968
Sse9I AATT 6 cut(s) 543, 599, 630, 935, 1031, 1036
SsiI CCGC 7 cut(s) 253, 292, 320, 393, 531, 557, 905
SspI AATATT 1 cut(s) 504
SspMI CTAG 4 cut(s) 362, 480, 858, 900
StyD4I CCNGG 2 cut(s) 10, 653
TaaI ACNGT 4 cut(s) 194, 428, 723, 967
TaqI TCGA 1 cut(s) 1073
TasI AATT 6 cut(s) 543, 599, 630, 935, 1031, 1036
TfiI GAWTC 2 cut(s) 638, 749
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TscAI CASTG 2 cut(s) 481, 592
TseI GCWGC 1 cut(s) 739
TspDTI ATGAA 3 cut(s) 299, 347, 732
TspGWI ACGGA 2 cut(s) 56, 810
TspRI CASTG 2 cut(s) 481, 592
Van91I CCANNNNNTGG 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 250, 776
XapI RAATTY 3 cut(s) 599, 935, 1036
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 4 cut(s) 362, 480, 858, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.