Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006993

Physical Location & Seq

Genomic Coordinates Reverse (-)

18792345 .. 18802143

Gene Structure 9799 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1080800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGGAGAGTTTGATATCGCTGGTGAACAAAATACAGCGGGCTTGCACAGCCCTCGGCGACCACGGCGAGGAGGGCGCCATGCCCACTCTCTGGGACTCCTTGCCCGCCATCGCCGTCGTTGGCGGCCAGAGCTCCGGCAAGTCTTCCGTGTTGGAGAGCGTCGTCGGCAAGGACTTTCTTCCTCGCGGTTCAGGAATTGTTACGCGGCGTCCTCTGGTTTTGCAGCTGCATAAGATCGACGAAGGAAGAGAATACGCTGAGTTTATGCACCTCCCCAGGAAGCGGTTCACCGATTTTGCTGCTGTGAGACAGGAGATATCTGATGAAACCGACAGAGAGACCGGCCGCTCGAAGGCAATTTCTACCGTTCCCATCCATCTTAGTATCTTCTCCCCTAACGTTGTGAACTTGACATTGATCGATCTTCCTGGGCTCACGAAAGTCGCTGTTGATGGTCAAGCAGAGAGCACAGTGAAGGACATTGAGGATATGGTTAGGTCCTTCATTGAGAAACCCAACTGTATTATTTTGGCTATTTCCCCTGCCAACCAAGATCTTGCTACTTCTGATGCAATTAAGATCTCTCGTGAAGTCGATCCTAAAGGGGAGAGGACATTTGGAGTTCTGACGAAGATTGATCTTATGGACCAGGGTACAAATGCAGTTGATATTTTGGAAGGAAGATCATATAAGCTACAGTTCCCCTGGATTGGCATTGTCAATCGCTCCCAAGCTGATATAAACAAGAGTGTTGATATGATTGCTGCTAGGCGTCGAGAGCGTGAATATTTTTCTGGTAGCCCAGAGTACAGGCATCTTGCAAACAGGATGGGCTCTGAGCATTTAGGCAGGGTGCTTTCTAAGCATCTAGAAGTTGTCATCAAGTCTCGAATTCCAGGCCTTCAGTCTCTTGTCAGCAAAACTATTGGTGAACTAGAGGCAGAACTAAGCCGACTCGGGAAGCCTATTGCTGCTGATGCTGGAGGAAAATTATATGTGATCATGGAAATCTCTCGTACTTTCGATCAGATATTTAAAGAACATCTGGATGGCGTGCGCTCTGGTGGTGAGAAAGTTTATGGAGTGTTTGACAATCAATTTCCAGCTGCTTTGAAACGGTTGCAGTTTGACAGGTATCTTTCTATGGATAACATCCGAAAGCTAATTACTGAAGCAGATGGATACCAGCCTCATCTAATTGCTCCTGAACAAGGTTACCGTCGCCTTATTGAATCTTCCTTGATTTGCATCAGAGGTCCTGCAGAGATGGCTGTTGATGCGGTTCATGGCATATTGAAGGATCTGGCTCAAAAATCTATCAACGAGACTACGGAACTAAAGCAGTATCCCACTTTAAGAGTAGAAGTTGCGAATGCAGCATTTGAGTCGCTGGAAAGGATGAAAGAAGAAAGCAAGAGAGCAACTCTACAGCTAGTAGATATGGAATGTGGTTACCTAACAGTTGACTTTTTCCGCAAGCTTCCTCAAGATATTGACAAGGGTGGAAATCCGACACACTCACTCTTCGATAGATACAATGATGCATACCTCCTGCGGGTTGGACACAATGTGTTAAGCTATGTCAACATGGTTTGTTCGAGTTTAAGGATTGCTATTCCAAAGTCCATCATCTACTGTCAAGTACGAGAGGCCAAACGCTGCTTGCTCGATCATTTCTTCGCAGAGTTGGGTGCAAAGGAGACAAGACAGTTGGCTAAGTTGCTAGACGAGGATCCGGCGGTAATGCAGAGGCGTACATTGCTTGCAAAGAGGCTAGAGTTGTACAGAAGTGCCCAGTTTGAGATTGAAGCAGTTGCTTGGTCTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000266 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007005 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0044424 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko04072 ko04144 ko04721 ko04961 ko05100 map04072 map04144 map04721 map04961 map05100

Pfam Domains

Protein Families

Dynamin_M Dynamin_N GED

Protein Analysis

610

Amino Acids

68.23

Weight (kDa)

6.92

Isoelectric Point (pI)

45.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dynamin_N	PF00350	37 - 212	3.8e-53	Dynamin family
Dynamin_M	PF01031	220 - 488	7.3e-60	Dynamin central region
GED	PF02212	519 - 605	3.3e-18	Dynamin GTPase effector domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006993)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61760 AT3G61760 AT3G61760
fragaria_vesca	FvH4_7g16440
malus_domestica	MD01G1080800.v1.1 MD07G1149800.v1.1
prunus_persica	Prupe.2G188500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0359481 RchiOBHm_Chr4g0423491 RchiOBHm_Chr5g0037431 RchiOBHm_Chr6g0269891
rosa_laevigata	RLG00000012393 RLG00000023120 RLG00000027866
rosa_multiflora	Rmu_sc0002556.1_g000007 Rmu_sc0006895.1_g000006
rosa_roxburghii	Rroxscaffold_4G00296970
rosa_rugosa	Rorug01G0271500
rosa_samantha	Rh1AG285000 Rh1BG251100 Rh1CG268900 Rh1DG280200 Rh2BG207000 Rh4DG017500
rosa_wichuraiana	Rw1G025340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	74
AccB7I	CCANNNNNTGG	1 cut(s)	90
AccBSI	CCGCTC	1 cut(s)	348
AccII	CGCG	2 cut(s)	186, 205
AclI	AACGTT	1 cut(s)	399
AclWI	GGATC	4 cut(s)	590, 1308, 1727, 1740
AcoI	YGGCCR	2 cut(s)	124, 343
AcsI	RAATTY	1 cut(s)	891
AcuI	CTGAAG	2 cut(s)	887, 1191
AcyI	GRCGYC	3 cut(s)	75, 208, 772
AdeI	CACNNNGTG	1 cut(s)	1570
AfaI	GTAC	6 cut(s)	655, 809, 1018, 1644, 1756, 1784
AfiI	CCNNNNNNNGG	7 cut(s)	67, 90, 282, 352, 710, 1211, 1555
AgsI	TTSAA	4 cut(s)	1114, 1232, 1297, 1808
AjnI	CCWGG	5 cut(s)	275, 427, 648, 704, 895
AluBI	AGCT	9 cut(s)	132, 226, 694, 734, 1106, 1162, 1432, 1480, 1578
AluI	AGCT	9 cut(s)	132, 226, 694, 734, 1106, 1162, 1432, 1480, 1578
Alw21I	GWGCWC	2 cut(s)	134, 470
Alw26I	GTCTC	6 cut(s)	301, 332, 891, 912, 1319, 1694
AlwI	GGATC	4 cut(s)	590, 1308, 1727, 1740
Ama87I	CYCGRG	1 cut(s)	956
AoxI	GGCC	4 cut(s)	124, 343, 898, 1650
ApeKI	GCWGC	8 cut(s)	223, 226, 299, 764, 971, 1106, 1376, 1659
ApoI	RAATTY	1 cut(s)	891
Asp700I	GAANNNNTTC	1 cut(s)	284
AspLEI	GCGC	2 cut(s)	77, 1059
AspS9I	GGNCC	3 cut(s)	498, 646, 1256
AsuHPI	GGTGA	4 cut(s)	34, 280, 941, 1079
AvaI	CYCGRG	1 cut(s)	956
AvaII	GGWCC	3 cut(s)	498, 646, 1256
BaeGI	GKGCMC	1 cut(s)	1795
BamHI	GGATCC	1 cut(s)	1732
BanI	GGYRCC	1 cut(s)	74
BanII	GRGCYC	3 cut(s)	134, 435, 836
BauI	CACGAG	1 cut(s)	585
BbsI	GAAGAC	1 cut(s)	135
Bbv12I	GWGCWC	2 cut(s)	134, 470
BbvI	GCAGC	8 cut(s)	213, 235, 286, 751, 958, 1093, 1388, 1646
BccI	CCATC	9 cut(s)	116, 380, 384, 446, 823, 1043, 1172, 1261, 1634
BceAI	ACGGC	2 cut(s)	79, 98
BcgI	CGANNNNNNTGC	6 cut(s)	34, 68, 281, 315, 1368, 1402
BciT130I	CCWGG	5 cut(s)	277, 429, 650, 706, 897
BciVI	GTATCC	2 cut(s)	1175, 1356
BclI	TGATCA	1 cut(s)	999
BcoDI	GTCTC	6 cut(s)	301, 332, 891, 912, 1319, 1694
BfaI	CTAG	6 cut(s)	768, 869, 935, 1433, 1724, 1775
BfmI	CTRYAG	3 cut(s)	695, 1260, 1427
BfoI	RGCGCY	1 cut(s)	78
BfuI	GTATCC	2 cut(s)	1175, 1356
BglII	AGATCT	2 cut(s)	553, 579
Bme1390I	CCNGG	5 cut(s)	277, 429, 650, 706, 897
Bme18I	GGWCC	3 cut(s)	498, 646, 1256
BmeT110I	CYCGRG	1 cut(s)	956
BmgT120I	GGNCC	3 cut(s)	498, 646, 1256
BmiI	GGNNCC	2 cut(s)	76, 1734
BmrFI	CCNGG	5 cut(s)	277, 429, 650, 706, 897
BmrI	ACTGGG	1 cut(s)	1789
BmsI	GCATC	7 cut(s)	559, 823, 874, 967, 1257, 1267, 1531
BmuI	ACTGGG	1 cut(s)	1789
BpiI	GAAGAC	1 cut(s)	135
BplI	GAGNNNNNCTC	2 cut(s)	1408, 1440
BpmI	CTGGAG	1 cut(s)	1002
BpuEI	CTTGAG	1 cut(s)	1470
Bsa29I	ATCGAT	1 cut(s)	420
BsaBI	GATNNNNATC	1 cut(s)	1247
BsaHI	GRCGYC	3 cut(s)	75, 208, 772
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	6 cut(s)	52, 61, 275, 428, 649, 704
Bsc4I	CCNNNNNNNGG	7 cut(s)	67, 90, 282, 352, 710, 1211, 1555
Bse118I	RCCGGY	1 cut(s)	341
Bse1I	ACTGG	1 cut(s)	1795
Bse3DI	GCAATG	1 cut(s)	1757
Bse8I	GATNNNNATC	1 cut(s)	1247
BseBI	CCWGG	5 cut(s)	277, 429, 650, 706, 897
BseCI	ATCGAT	1 cut(s)	420
BseDI	CCNNGG	6 cut(s)	52, 61, 275, 428, 649, 704
BseGI	GGATG	5 cut(s)	372, 834, 1054, 1152, 1404
BseJI	GATNNNNATC	1 cut(s)	1247
BseLI	CCNNNNNNNGG	7 cut(s)	67, 90, 282, 352, 710, 1211, 1555
BseMI	GCAATG	1 cut(s)	1757
BseMII	CTCAG	2 cut(s)	249, 828
BseNI	ACTGG	1 cut(s)	1795
BseRI	GAGGAG	1 cut(s)	83
BseSI	GKGCMC	1 cut(s)	1795
BseX3I	CGGCCG	1 cut(s)	343
BseXI	GCAGC	8 cut(s)	213, 235, 286, 751, 958, 1093, 1388, 1646
Bsh1236I	CGCG	2 cut(s)	186, 205
Bsh1285I	CGRYCG	1 cut(s)	346
BshFI	GGCC	4 cut(s)	126, 345, 900, 1652
BshNI	GGYRCC	1 cut(s)	74
BshVI	ATCGAT	1 cut(s)	420
BsiEI	CGRYCG	1 cut(s)	346
BsiHKAI	GWGCWC	2 cut(s)	134, 470
BsiHKCI	CYCGRG	1 cut(s)	956
BsiSI	CCGG	3 cut(s)	135, 342, 1736
BslFI	GGGAC	1 cut(s)	107
BslI	CCNNNNNNNGG	7 cut(s)	67, 90, 282, 352, 710, 1211, 1555
BsmAI	GTCTC	6 cut(s)	301, 332, 891, 912, 1319, 1694
BsmFI	GGGAC	1 cut(s)	107
BsmI	GAATGC	1 cut(s)	1378
BsnI	GGCC	4 cut(s)	126, 345, 900, 1652
Bso31I	GGTCTC	1 cut(s)	332
BsoBI	CYCGRG	1 cut(s)	956
Bsp1286I	GDGCHC	5 cut(s)	134, 435, 470, 836, 1795
Bsp1407I	TGTACA	1 cut(s)	1782
BspANI	GGCC	4 cut(s)	126, 345, 900, 1652
BspCNI	CTCAG	2 cut(s)	250, 829
BspDI	ATCGAT	1 cut(s)	420
BspFNI	CGCG	2 cut(s)	186, 205
BspLI	GGNNCC	2 cut(s)	76, 1734
BspMAI	CTGCAG	1 cut(s)	1264
BspPI	GGATC	4 cut(s)	590, 1308, 1727, 1740
BspT107I	GGYRCC	1 cut(s)	74
BspTNI	GGTCTC	1 cut(s)	332
BsrBI	CCGCTC	1 cut(s)	348
BsrDI	GCAATG	1 cut(s)	1757
BsrFI	RCCGGY	1 cut(s)	341
BsrGI	TGTACA	1 cut(s)	1782
BsrI	ACTGG	1 cut(s)	1795
BssAI	RCCGGY	1 cut(s)	341
BssECI	CCNNGG	6 cut(s)	52, 61, 275, 428, 649, 704
BssNI	GRCGYC	3 cut(s)	75, 208, 772
BssSI	CACGAG	1 cut(s)	585
Bst2BI	CACGAG	1 cut(s)	585
Bst2UI	CCWGG	5 cut(s)	277, 429, 650, 706, 897
Bst4CI	ACNGT	9 cut(s)	367, 472, 521, 699, 1119, 1220, 1462, 1637, 1710
Bst6I	CTCTTC	2 cut(s)	241, 1529
BstACI	GRCGYC	3 cut(s)	75, 208, 772
BstAUI	TGTACA	1 cut(s)	1782
BstC8I	GCNNGC	7 cut(s)	39, 43, 105, 1055, 1478, 1664, 1764
BstDEI	CTNAG	7 cut(s)	258, 380, 837, 861, 947, 1716, 1824
BstDSI	CCRYGG	1 cut(s)	61
BstEII	GGTNACC	2 cut(s)	1214, 1451
BstENI	CCTNNNNNAGG	1 cut(s)	1209
BstF5I	GGATG	5 cut(s)	372, 834, 1054, 1152, 1404
BstFNI	CGCG	2 cut(s)	186, 205
BstH2I	RGCGCY	1 cut(s)	78
BstHHI	GCGC	2 cut(s)	77, 1059
BstMAI	GTCTC	6 cut(s)	301, 332, 891, 912, 1319, 1694
BstMCI	CGRYCG	1 cut(s)	346
BstNI	CCWGG	5 cut(s)	277, 429, 650, 706, 897
BstPI	GGTNACC	2 cut(s)	1214, 1451
BstSCI	CCNGG	5 cut(s)	275, 427, 648, 704, 895
BstSFI	CTRYAG	3 cut(s)	695, 1260, 1427
BstSLI	GKGCMC	1 cut(s)	1795
BstUI	CGCG	2 cut(s)	186, 205
BstV1I	GCAGC	8 cut(s)	213, 235, 286, 751, 958, 1093, 1388, 1646
BstV2I	GAAGAC	1 cut(s)	135
BstX2I	RGATCY	4 cut(s)	553, 579, 1300, 1732
BstYI	RGATCY	4 cut(s)	553, 579, 1300, 1732
BstZI	CGGCCG	1 cut(s)	343
Bsu15I	ATCGAT	1 cut(s)	420
BsuI	GTATCC	2 cut(s)	1175, 1356
BsuRI	GGCC	4 cut(s)	126, 345, 900, 1652
BsuTUI	ATCGAT	1 cut(s)	420
BtgI	CCRYGG	1 cut(s)	61
BtgZI	GCGATG	1 cut(s)	94
BtsCI	GGATG	5 cut(s)	372, 834, 1054, 1152, 1404
BtsIMutI	CAGTG	1 cut(s)	477
Cac8I	GCNNGC	7 cut(s)	39, 43, 105, 1055, 1478, 1664, 1764
CfoI	GCGC	2 cut(s)	77, 1059
Cfr10I	RCCGGY	1 cut(s)	341
Cfr13I	GGNCC	3 cut(s)	498, 646, 1256
ClaI	ATCGAT	1 cut(s)	420
CseI	GACGC	3 cut(s)	148, 197, 761
Csp6I	GTAC	6 cut(s)	654, 808, 1017, 1643, 1755, 1783
CviAII	CATG	4 cut(s)	79, 1003, 1286, 1588
CviQI	GTAC	6 cut(s)	654, 808, 1017, 1643, 1755, 1783
DdeI	CTNAG	7 cut(s)	258, 380, 837, 861, 947, 1716, 1824
DinI	GGCGCC	1 cut(s)	76
DraI	TTTAAA	1 cut(s)	1036
DraIII	CACNNNGTG	1 cut(s)	1570
EaeI	YGGCCR	2 cut(s)	124, 343
EagI	CGGCCG	1 cut(s)	343
Eam1104I	CTCTTC	2 cut(s)	241, 1529
EarI	CTCTTC	2 cut(s)	241, 1529
Ecl136II	GAGCTC	1 cut(s)	132
EclXI	CGGCCG	1 cut(s)	343
Eco147I	AGGCCT	1 cut(s)	900
Eco24I	GRGCYC	3 cut(s)	134, 435, 836
Eco31I	GGTCTC	1 cut(s)	332
Eco32I	GATATC	2 cut(s)	15, 318
Eco47I	GGWCC	3 cut(s)	498, 646, 1256
Eco52I	CGGCCG	1 cut(s)	343
Eco53kI	GAGCTC	1 cut(s)	132
Eco57I	CTGAAG	2 cut(s)	887, 1191
Eco88I	CYCGRG	1 cut(s)	956
Eco91I	GGTNACC	2 cut(s)	1214, 1451
EcoICRI	GAGCTC	1 cut(s)	132
EcoNI	CCTNNNNNAGG	1 cut(s)	1209
EcoO109I	RGGNCCY	2 cut(s)	498, 1256
EcoO65I	GGTNACC	2 cut(s)	1214, 1451
EcoRI	GAATTC	1 cut(s)	891
EcoRII	CCWGG	5 cut(s)	275, 427, 648, 704, 895
EcoRV	GATATC	2 cut(s)	15, 318
EcoT22I	ATGCAT	1 cut(s)	1546
EcoT38I	GRGCYC	3 cut(s)	134, 435, 836
EgeI	GGCGCC	1 cut(s)	76
EheI	GGCGCC	1 cut(s)	76
FaeI	CATG	4 cut(s)	82, 1006, 1289, 1591
FaqI	GGGAC	1 cut(s)	107
FatI	CATG	4 cut(s)	78, 1002, 1285, 1587
FauI	CCCGC	3 cut(s)	30, 112, 1548
FbaI	TGATCA	1 cut(s)	999
FokI	GGATG	5 cut(s)	359, 841, 1061, 1139, 1411
FriOI	GRGCYC	3 cut(s)	134, 435, 836
FspBI	CTAG	6 cut(s)	768, 869, 935, 1433, 1724, 1775
GlaI	GCGC	2 cut(s)	76, 1058
GsuI	CTGGAG	1 cut(s)	1002
HaeII	RGCGCY	1 cut(s)	78
HaeIII	GGCC	4 cut(s)	126, 345, 900, 1652
HapII	CCGG	3 cut(s)	135, 342, 1736
HgaI	GACGC	3 cut(s)	148, 197, 761
HhaI	GCGC	2 cut(s)	77, 1059
Hin1I	GRCGYC	3 cut(s)	75, 208, 772
Hin1II	CATG	4 cut(s)	82, 1006, 1289, 1591
Hin6I	GCGC	2 cut(s)	75, 1057
HinP1I	GCGC	2 cut(s)	75, 1057
HincII	GTYRAC	2 cut(s)	1465, 1585
HindII	GTYRAC	2 cut(s)	1465, 1585
HindIII	AAGCTT	1 cut(s)	1478
HinfI	GANTC	4 cut(s)	95, 954, 1232, 1385
HpaII	CCGG	3 cut(s)	135, 342, 1736
HphI	GGTGA	4 cut(s)	34, 280, 941, 1079
Hpy166II	GTNNAC	6 cut(s)	25, 288, 406, 932, 1465, 1585
Hpy188I	TCNGA	8 cut(s)	322, 568, 627, 838, 1029, 1157, 1253, 1512
Hpy8I	GTNNAC	6 cut(s)	25, 288, 406, 932, 1465, 1585
Hpy99I	CGWCG	6 cut(s)	119, 164, 167, 242, 777, 1224
HpyAV	CCTTC	7 cut(s)	236, 346, 469, 511, 671, 911, 1291
HpyCH4III	ACNGT	9 cut(s)	367, 472, 521, 699, 1119, 1220, 1462, 1637, 1710
HpyCH4IV	ACGT	1 cut(s)	399
HpyF3I	CTNAG	7 cut(s)	258, 380, 837, 861, 947, 1716, 1824
HpySE526I	ACGT	1 cut(s)	399
Hsp92I	GRCGYC	3 cut(s)	75, 208, 772
Hsp92II	CATG	4 cut(s)	82, 1006, 1289, 1591
HspAI	GCGC	2 cut(s)	75, 1057
KasI	GGCGCC	1 cut(s)	74
Ksp22I	TGATCA	1 cut(s)	999
LmnI	GCTCC	3 cut(s)	137, 731, 1207
Lsp1109I	GCAGC	8 cut(s)	213, 235, 286, 751, 958, 1093, 1388, 1646
LweI	GCATC	7 cut(s)	559, 823, 874, 967, 1257, 1267, 1531
MaeI	CTAG	6 cut(s)	768, 869, 935, 1433, 1724, 1775
MaeII	ACGT	1 cut(s)	399
MaeIII	GTNAC	3 cut(s)	199, 1214, 1451
MbiI	CCGCTC	1 cut(s)	348
MflI	RGATCY	4 cut(s)	553, 579, 1300, 1732
MhlI	GDGCHC	5 cut(s)	134, 435, 470, 836, 1795
MluCI	AATT	8 cut(s)	195, 357, 573, 891, 989, 1097, 1164, 1197
Mly113I	GGCGCC	1 cut(s)	75
MlyI	GAGTC	3 cut(s)	89, 948, 1394
MmeI	TCCRAC	3 cut(s)	132, 1535, 1540
Mph1103I	ATGCAT	1 cut(s)	1546
MroXI	GAANNNNTTC	1 cut(s)	284
MseI	TTAA	5 cut(s)	576, 1035, 1355, 1574, 1604
MslI	CAYNNNNRTG	1 cut(s)	1047
MspA1I	CMGCKG	3 cut(s)	37, 226, 1106
MspI	CCGG	3 cut(s)	135, 342, 1736
MspR9I	CCNGG	5 cut(s)	277, 429, 650, 706, 897
Mva1269I	GAATGC	1 cut(s)	1378
MvaI	CCWGG	5 cut(s)	277, 429, 650, 706, 897
MvnI	CGCG	2 cut(s)	186, 205
NarI	GGCGCC	1 cut(s)	75
NlaIII	CATG	4 cut(s)	82, 1006, 1289, 1591
NlaIV	GGNNCC	2 cut(s)	76, 1734
NmeAIII	GCCGAG	1 cut(s)	33
NsiI	ATGCAT	1 cut(s)	1546
PceI	AGGCCT	1 cut(s)	900
PctI	GAATGC	1 cut(s)	1378
PdmI	GAANNNNTTC	1 cut(s)	284
PfeI	GAWTC	1 cut(s)	1232
PflMI	CCANNNNNTGG	1 cut(s)	90
PleI	GAGTC	3 cut(s)	89, 948, 1393
PluTI	GGCGCC	1 cut(s)	78
PpsI	GAGTC	3 cut(s)	89, 948, 1393
PpuMI	RGGWCCY	2 cut(s)	498, 1256
Psp124BI	GAGCTC	1 cut(s)	134
Psp1406I	AACGTT	1 cut(s)	399
Psp5II	RGGWCCY	2 cut(s)	498, 1256
Psp6I	CCWGG	5 cut(s)	275, 427, 648, 704, 895
PspEI	GGTNACC	2 cut(s)	1214, 1451
PspGI	CCWGG	5 cut(s)	275, 427, 648, 704, 895
PspN4I	GGNNCC	2 cut(s)	76, 1734
PspPI	GGNCC	3 cut(s)	498, 646, 1256
PspPPI	RGGWCCY	2 cut(s)	498, 1256
PstI	CTGCAG	1 cut(s)	1264
PsuI	RGATCY	4 cut(s)	553, 579, 1300, 1732
PvuII	CAGCTG	2 cut(s)	226, 1106
RsaI	GTAC	6 cut(s)	655, 809, 1018, 1644, 1756, 1784
RsaNI	GTAC	6 cut(s)	654, 808, 1017, 1643, 1755, 1783
RseI	CAYNNNNRTG	1 cut(s)	1047
SacI	GAGCTC	1 cut(s)	134
SaqAI	TTAA	5 cut(s)	576, 1035, 1355, 1574, 1604
Sau96I	GGNCC	3 cut(s)	498, 646, 1256
SchI	GAGTC	3 cut(s)	89, 948, 1394
ScrFI	CCNGG	5 cut(s)	277, 429, 650, 706, 897
SduI	GDGCHC	5 cut(s)	134, 435, 470, 836, 1795
SfaNI	GCATC	7 cut(s)	559, 823, 874, 967, 1257, 1267, 1531
SfcI	CTRYAG	3 cut(s)	695, 1260, 1427
SfoI	GGCGCC	1 cut(s)	76
SinI	GGWCC	3 cut(s)	498, 646, 1256
SmiMI	CAYNNNNRTG	1 cut(s)	1047
SmlI	CTYRAG	1 cut(s)	1485
SmoI	CTYRAG	1 cut(s)	1485
Sse9I	AATT	8 cut(s)	195, 357, 573, 891, 989, 1097, 1164, 1197
SseBI	AGGCCT	1 cut(s)	900
SspDI	GGCGCC	1 cut(s)	74
SspI	AATATT	1 cut(s)	788
SspMI	CTAG	6 cut(s)	768, 869, 935, 1433, 1724, 1775
SstI	GAGCTC	1 cut(s)	134
StuI	AGGCCT	1 cut(s)	900
StyD4I	CCNGG	5 cut(s)	275, 427, 648, 704, 895
TaaI	ACNGT	9 cut(s)	367, 472, 521, 699, 1119, 1220, 1462, 1637, 1710
TaiI	ACGT	1 cut(s)	402
TasI	AATT	8 cut(s)	195, 357, 573, 891, 989, 1097, 1164, 1197
TatI	WGTACW	2 cut(s)	807, 1782
TauI	GCSGC	3 cut(s)	126, 208, 348
TfiI	GAWTC	1 cut(s)	1232
Tru1I	TTAA	5 cut(s)	576, 1035, 1355, 1574, 1604
Tru9I	TTAA	5 cut(s)	576, 1035, 1355, 1574, 1604
TscAI	CASTG	1 cut(s)	477
TseI	GCWGC	8 cut(s)	223, 226, 299, 764, 971, 1106, 1376, 1659
TspDTI	ATGAA	4 cut(s)	339, 493, 1274, 1415
TspGWI	ACGGA	2 cut(s)	136, 1346
TspRI	CASTG	1 cut(s)	477
Van91I	CCANNNNNTGG	1 cut(s)	90
VpaK11BI	GGWCC	3 cut(s)	498, 646, 1256
XagI	CCTNNNNNAGG	1 cut(s)	1209
XapI	RAATTY	1 cut(s)	891
XbaI	TCTAGA	1 cut(s)	868
XmnI	GAANNNNTTC	1 cut(s)	284
XspI	CTAG	6 cut(s)	768, 869, 935, 1433, 1724, 1775
Zsp2I	ATGCAT	1 cut(s)	1546

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.