Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0006993

Physical Location & Seq

Genomic Coordinates Reverse (-)

51945594 .. 51951135

Gene Structure 5542 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G025340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGGAGAATCTGATCTCGCTCGTGAACAAAATACAGAGAGCATGCACAGCCCTCGGCGATCACGGCGAAGAGAGCGCAATGCCGACTCTCTGGGACTCCCTCCCCGCCATCGCCGTAGTCGGCGGCCAGAGCTCCGGTAAGTCGTCGGTGCTGGAGAGCATTGTCGGCAAAGACTTCCTACCTCGCGGCTCCGGAATTGTGACTCGGCGTCCGCTTGTGTTGCAGCTGCATAAGATCGACGAGGGAAGAGAATACGCCGAGTTTATGCACCTCCCGAGGAAGAGGTTCACTGATTTTGCTGCTGTTAGACAGGAGATATCTGATGAGACTGATAGAGAGACCGGCCGGACCAAGGCGATTTCGAGTGTGCCTATCCATCTTAGCATCTACTCCCCTAATGTTGTGAACTTGACACTAATAGATCTTCCTGGACTCACAAAGGTAGCTGTAGAGGGTCAGGCAGAGAGCATAGTGCAGGACATTGAGAACATGGTTAGGGGCTTCATTGAGAAGCCCAACTGTATCATTCTGGCTATTTCCCCTGCCAACCAAGATCTTGCTACGTCTGATGCAATTAAGATCTCTCGTGAAGTAGATCCCAAAGGTGAGAGGACATTTGGAGTTCTCACAAAGATTGATCTTATGGACCAGGGTACAAATGCAGTCGATATTTTGGAAGGAAGATCATATAGACTTCAGTTCCCATGGATTGGTGTTGTGAATCGCTCCCAAGCTGATATAAACAAGAGTGTTGATATGATTGCTGCTAGGCGCAGGGAGCGTGAATATTTTGCTAATATCCCCGAGTACAAGCATCTTGCAAGCAAGATGGGATCTGAGCATTTAGGAAGGGTACTGTCTAAGCATTTGGAAGTTGTCATCAAGTCCAGAATTCCAGGCCTTCAGTCTCTTATCAGCAAAACTATTGGTGAGCTAGAGGCAGAACTAAGCCGACTTGGGAAGCCCATTGCTTCTGATACTGGTGGAAAGTTGTATGTAATAATGGAAATCTCTCGTACTTTCGATCAGATATTTAAAGAACATCTTGATGGCGTGCGCTCTGGTGGTGATAAAGTTTATGGTGTGTTTGACAATCAATTTCCAGCTGCTTTGAAGAGATTGCAATTTGACAAGTATCTTTCAATGGAAAACATTCGAAAGCTAATTACTGAGGCAGATGGATATCAACCTCATTTAATTGCTCCTGAACAAGGATACCGTCGCCTCATTGAATCTTCATTAGTATGCATCAAAGGTCCTGCAGAGGCGGCTGTTGATGCTGTTCATGCCATATTGAAGGAGCTGGCTCAGAAGTCTATCAATGCAACCTCAGAATTAAAGCAGTATCCCACTTTAAGAGTGGAAGTTGGGAATGCAGCATTTGAATCGCTGGATAGGATGAAAGAAGAGAGCAAGAAAGCAACCATGCAGCTAGTTGATATGGAGTGTGGTTACCTCACTGTTGAATTTTTCCGCAAGCTTCCTCAGGATATTGAAAAGGGGGGAAATCCAACACACTCACTTTTCGACAGATATAACGATTCCTACCTCCGGCGGGTTGGAAGCAATGTGTTAAACTATGTGAACATGGTGTGCTCAAGTTTAAGGATTGCTATTCCAAAGTCTATTGTCTACTGTCAAGTACGAGAAGCCAAACGCAGCTTACTCGATCATTTTTTTGCTGAGCTGGGTTCAAAAGAGACAAGACAGTTGTCTAAGATGTTGGATGAGGATCCAGCAGTCATGCAGCGTCGTACCAGCCTTGCAAAGAGGCTAGAGCTGTACAGAAGTGCTCAGGCAGAGATTGATGCAGTTGCTTGGTCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dynamin_M Dynamin_N GED

Protein Analysis

609

Amino Acids

68.13

Weight (kDa)

7.63

Isoelectric Point (pI)

45.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dynamin_N	PF00350	37 - 212	5.4e-54	Dynamin family
Dynamin_M	PF01031	220 - 487	3.7e-62	Dynamin central region
GED	PF02212	513 - 605	1.4e-20	Dynamin GTPase effector domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006993)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61760 AT3G61760 AT3G61760
fragaria_vesca	FvH4_7g16440
malus_domestica	MD01G1080800.v1.1 MD07G1149800.v1.1
prunus_persica	Prupe.2G188500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0359481 RchiOBHm_Chr4g0423491 RchiOBHm_Chr5g0037431 RchiOBHm_Chr6g0269891
rosa_laevigata	RLG00000012393 RLG00000023120 RLG00000027866
rosa_multiflora	Rmu_sc0002556.1_g000007 Rmu_sc0006895.1_g000006
rosa_roxburghii	Rroxscaffold_4G00296970
rosa_rugosa	Rorug01G0271500
rosa_samantha	Rh1AG285000 Rh1BG251100 Rh1CG268900 Rh1DG280200 Rh2BG207000 Rh4DG017500
rosa_wichuraiana	Rw1G025340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	710
AccI	GTMKAC	1 cut(s)	1632
AccII	CGCG	1 cut(s)	186
AccIII	TCCGGA	1 cut(s)	191
AciI	CCGC	7 cut(s)	105, 123, 186, 212, 1268, 1474, 1555
AclWI	GGATC	4 cut(s)	590, 841, 1727, 1740
AcoI	YGGCCR	2 cut(s)	124, 343
AcsI	RAATTY	2 cut(s)	891, 1466
AcuI	CTGAAG	2 cut(s)	680, 887
AcyI	GRCGYC	1 cut(s)	208
AfaI	GTAC	7 cut(s)	655, 809, 855, 1018, 1644, 1756, 1784
AfiI	CCNNNNNNNGG	5 cut(s)	352, 710, 1211, 1551, 1555
AgsI	TTSAA	8 cut(s)	1114, 1143, 1232, 1297, 1385, 1466, 1496, 1695
AhdI	GACNNNNNGTC	1 cut(s)	207
AjnI	CCWGG	3 cut(s)	427, 648, 895
Alw21I	GWGCWC	3 cut(s)	134, 1598, 1795
Alw26I	GTCTC	4 cut(s)	320, 332, 912, 1694
AlwI	GGATC	4 cut(s)	590, 841, 1727, 1740
Ama87I	CYCGRG	2 cut(s)	274, 803
Aor13HI	TCCGGA	1 cut(s)	191
AoxI	GGCC	3 cut(s)	124, 343, 898
ApeKI	GCWGC	9 cut(s)	223, 226, 299, 764, 1106, 1376, 1429, 1659, 1747
ApoI	RAATTY	2 cut(s)	891, 1466
Asp700I	GAANNNNTTC	2 cut(s)	284, 1152
AspLEI	GCGC	3 cut(s)	77, 774, 1059
AspS9I	GGNCC	4 cut(s)	348, 646, 1256, 1821
AsuHPI	GGTGA	3 cut(s)	617, 941, 1079
AsuII	TTCGAA	1 cut(s)	1156
AvaI	CYCGRG	2 cut(s)	274, 803
AvaII	GGWCC	4 cut(s)	348, 646, 1256, 1821
AxyI	CCTNAGG	1 cut(s)	1485
BamHI	GGATCC	1 cut(s)	1732
BanII	GRGCYC	1 cut(s)	134
BauI	CACGAG	2 cut(s)	20, 585
Bbv12I	GWGCWC	3 cut(s)	134, 1598, 1795
BbvI	GCAGC	9 cut(s)	213, 235, 286, 751, 1093, 1388, 1441, 1671, 1759
BccI	CCATC	5 cut(s)	116, 384, 823, 1043, 1172
BceAI	ACGGC	2 cut(s)	79, 98
BcgI	CGANNNNNNTGC	6 cut(s)	34, 68, 1368, 1402, 1648, 1682
BciT130I	CCWGG	3 cut(s)	429, 650, 897
BciVI	GTATCC	2 cut(s)	1208, 1356
BcoDI	GTCTC	4 cut(s)	320, 332, 912, 1694
BfaI	CTAG	4 cut(s)	768, 935, 1433, 1775
BfmI	CTRYAG	2 cut(s)	447, 1260
BfuI	GTATCC	2 cut(s)	1208, 1356
BglII	AGATCT	3 cut(s)	421, 553, 579
BlpI	GCTNAGC	1 cut(s)	1683
Bme1390I	CCNGG	3 cut(s)	429, 650, 897
Bme18I	GGWCC	4 cut(s)	348, 646, 1256, 1821
BmeRI	GACNNNNNGTC	1 cut(s)	207
BmeT110I	CYCGRG	2 cut(s)	274, 803
BmgT120I	GGNCC	4 cut(s)	348, 646, 1256, 1821
BmiI	GGNNCC	2 cut(s)	190, 1734
BmrFI	CCNGG	3 cut(s)	429, 650, 897
BmsI	GCATC	6 cut(s)	393, 559, 823, 1257, 1267, 1798
BoxI	GACNNNNGTC	1 cut(s)	1711
BpmI	CTGGAG	1 cut(s)	173
Bpu10I	CCTNAGC	1 cut(s)	1794
Bpu1102I	GCTNAGC	1 cut(s)	1683
Bpu14I	TTCGAA	1 cut(s)	1156
BpuEI	CTTGAG	1 cut(s)	1582
BsaBI	GATNNNNATC	2 cut(s)	1182, 1731
BsaHI	GRCGYC	1 cut(s)	208
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	5 cut(s)	52, 275, 351, 649, 704
BsaWI	WCCGGW	2 cut(s)	134, 191
Bsc4I	CCNNNNNNNGG	5 cut(s)	352, 710, 1211, 1551, 1555
Bse118I	RCCGGY	1 cut(s)	341
Bse1I	ACTGG	1 cut(s)	985
Bse21I	CCTNAGG	1 cut(s)	1485
Bse3DI	GCAATG	3 cut(s)	84, 966, 1573
Bse8I	GATNNNNATC	2 cut(s)	1182, 1731
BseAI	TCCGGA	1 cut(s)	191
BseBI	CCWGG	3 cut(s)	429, 650, 897
BseDI	CCNNGG	5 cut(s)	52, 275, 351, 649, 704
BseGI	GGATG	2 cut(s)	1404, 1732
BseJI	GATNNNNATC	2 cut(s)	1182, 1731
BseLI	CCNNNNNNNGG	5 cut(s)	352, 710, 1211, 1551, 1555
BseMI	GCAATG	3 cut(s)	84, 966, 1573
BseMII	CTCAG	7 cut(s)	828, 1161, 1322, 1344, 1499, 1674, 1808
BseNI	ACTGG	1 cut(s)	985
BseX3I	CGGCCG	1 cut(s)	343
BseXI	GCAGC	9 cut(s)	213, 235, 286, 751, 1093, 1388, 1441, 1671, 1759
BseYI	CCCAGC	1 cut(s)	1687
BsgI	GTGCAG	1 cut(s)	494
Bsh1236I	CGCG	1 cut(s)	186
Bsh1285I	CGRYCG	1 cut(s)	346
BshFI	GGCC	3 cut(s)	126, 345, 900
BsiEI	CGRYCG	1 cut(s)	346
BsiHKAI	GWGCWC	3 cut(s)	134, 1598, 1795
BsiHKCI	CYCGRG	2 cut(s)	274, 803
BsiSI	CCGG	5 cut(s)	135, 192, 342, 346, 1552
BslFI	GGGAC	1 cut(s)	107
BslI	CCNNNNNNNGG	5 cut(s)	352, 710, 1211, 1551, 1555
BsmAI	GTCTC	4 cut(s)	320, 332, 912, 1694
BsmFI	GGGAC	1 cut(s)	107
BsmI	GAATGC	1 cut(s)	1378
BsnI	GGCC	3 cut(s)	126, 345, 900
Bso31I	GGTCTC	1 cut(s)	332
BsoBI	CYCGRG	2 cut(s)	274, 803
Bsp119I	TTCGAA	1 cut(s)	1156
Bsp1286I	GDGCHC	3 cut(s)	134, 1598, 1795
Bsp13I	TCCGGA	1 cut(s)	191
Bsp1407I	TGTACA	1 cut(s)	1782
Bsp1720I	GCTNAGC	1 cut(s)	1683
Bsp19I	CCATGG	1 cut(s)	704
BspACI	CCGC	7 cut(s)	105, 123, 186, 212, 1268, 1474, 1555
BspANI	GGCC	3 cut(s)	126, 345, 900
BspCNI	CTCAG	7 cut(s)	829, 1162, 1321, 1343, 1498, 1675, 1807
BspEI	TCCGGA	1 cut(s)	191
BspFNI	CGCG	1 cut(s)	186
BspLI	GGNNCC	2 cut(s)	190, 1734
BspMAI	CTGCAG	1 cut(s)	1264
BspPI	GGATC	4 cut(s)	590, 841, 1727, 1740
BspT104I	TTCGAA	1 cut(s)	1156
BspTNI	GGTCTC	1 cut(s)	332
BsrDI	GCAATG	3 cut(s)	84, 966, 1573
BsrFI	RCCGGY	1 cut(s)	341
BsrGI	TGTACA	1 cut(s)	1782
BsrI	ACTGG	1 cut(s)	985
BssAI	RCCGGY	1 cut(s)	341
BssECI	CCNNGG	5 cut(s)	52, 275, 351, 649, 704
BssNI	GRCGYC	1 cut(s)	208
BssSI	CACGAG	2 cut(s)	20, 585
BssT1I	CCWWGG	2 cut(s)	351, 704
Bst2BI	CACGAG	2 cut(s)	20, 585
Bst2UI	CCWGG	3 cut(s)	429, 650, 897
Bst4CI	ACNGT	6 cut(s)	521, 858, 1220, 1462, 1637, 1710
Bst6I	CTCTTC	5 cut(s)	63, 241, 275, 1109, 1401
BstACI	GRCGYC	1 cut(s)	208
BstAUI	TGTACA	1 cut(s)	1782
BstBI	TTCGAA	1 cut(s)	1156
BstC8I	GCNNGC	5 cut(s)	43, 823, 1055, 1305, 1478
BstDSI	CCRYGG	1 cut(s)	704
BstEII	GGTNACC	1 cut(s)	1451
BstENI	CCTNNNNNAGG	1 cut(s)	1209
BstF5I	GGATG	2 cut(s)	1404, 1732
BstFNI	CGCG	1 cut(s)	186
BstHHI	GCGC	3 cut(s)	77, 774, 1059
BstMAI	GTCTC	4 cut(s)	320, 332, 912, 1694
BstMCI	CGRYCG	1 cut(s)	346
BstNI	CCWGG	3 cut(s)	429, 650, 897
BstNSI	RCATGY	1 cut(s)	45
BstPAI	GACNNNNGTC	1 cut(s)	1711
BstPI	GGTNACC	1 cut(s)	1451
BstSCI	CCNGG	3 cut(s)	427, 648, 895
BstSFI	CTRYAG	2 cut(s)	447, 1260
BstUI	CGCG	1 cut(s)	186
BstV1I	GCAGC	9 cut(s)	213, 235, 286, 751, 1093, 1388, 1441, 1671, 1759
BstX2I	RGATCY	6 cut(s)	421, 553, 579, 595, 833, 1732
BstYI	RGATCY	6 cut(s)	421, 553, 579, 595, 833, 1732
BstZI	CGGCCG	1 cut(s)	343
Bsu36I	CCTNAGG	1 cut(s)	1485
BsuI	GTATCC	2 cut(s)	1208, 1356
BsuRI	GGCC	3 cut(s)	126, 345, 900
BtgI	CCRYGG	1 cut(s)	704
BtgZI	GCGATG	1 cut(s)	94
BtsCI	GGATG	2 cut(s)	1404, 1732
BtsIMutI	CAGTG	2 cut(s)	288, 1458
Cac8I	GCNNGC	5 cut(s)	43, 823, 1055, 1305, 1478
CfoI	GCGC	3 cut(s)	77, 774, 1059
Cfr10I	RCCGGY	1 cut(s)	341
Cfr13I	GGNCC	4 cut(s)	348, 646, 1256, 1821
CseI	GACGC	2 cut(s)	197, 1739
Csp6I	GTAC	7 cut(s)	654, 808, 854, 1017, 1643, 1755, 1783
CviAII	CATG	7 cut(s)	42, 490, 705, 1286, 1426, 1588, 1744
CviQI	GTAC	7 cut(s)	654, 808, 854, 1017, 1643, 1755, 1783
DraI	TTTAAA	1 cut(s)	1036
DriI	GACNNNNNGTC	1 cut(s)	207
EaeI	YGGCCR	2 cut(s)	124, 343
EagI	CGGCCG	1 cut(s)	343
Eam1104I	CTCTTC	5 cut(s)	63, 241, 275, 1109, 1401
Eam1105I	GACNNNNNGTC	1 cut(s)	207
EarI	CTCTTC	5 cut(s)	63, 241, 275, 1109, 1401
Ecl136II	GAGCTC	1 cut(s)	132
EclXI	CGGCCG	1 cut(s)	343
Eco130I	CCWWGG	2 cut(s)	351, 704
Eco147I	AGGCCT	1 cut(s)	900
Eco24I	GRGCYC	1 cut(s)	134
Eco31I	GGTCTC	1 cut(s)	332
Eco32I	GATATC	2 cut(s)	318, 1184
Eco47I	GGWCC	4 cut(s)	348, 646, 1256, 1821
Eco52I	CGGCCG	1 cut(s)	343
Eco53kI	GAGCTC	1 cut(s)	132
Eco57I	CTGAAG	2 cut(s)	680, 887
Eco81I	CCTNAGG	1 cut(s)	1485
Eco88I	CYCGRG	2 cut(s)	274, 803
Eco91I	GGTNACC	1 cut(s)	1451
EcoICRI	GAGCTC	1 cut(s)	132
EcoNI	CCTNNNNNAGG	1 cut(s)	1209
EcoO109I	RGGNCCY	1 cut(s)	1256
EcoO65I	GGTNACC	1 cut(s)	1451
EcoRI	GAATTC	1 cut(s)	891
EcoRII	CCWGG	3 cut(s)	427, 648, 895
EcoRV	GATATC	2 cut(s)	318, 1184
EcoT14I	CCWWGG	2 cut(s)	351, 704
EcoT22I	ATGCAT	1 cut(s)	1250
EcoT38I	GRGCYC	1 cut(s)	134
ErhI	CCWWGG	2 cut(s)	351, 704
FaeI	CATG	7 cut(s)	45, 493, 708, 1289, 1429, 1591, 1747
FalI	AAGNNNNNCTT	2 cut(s)	1029, 1061
FaqI	GGGAC	1 cut(s)	107
FatI	CATG	7 cut(s)	41, 489, 704, 1285, 1425, 1587, 1743
FauI	CCCGC	2 cut(s)	112, 1548
FblI	GTMKAC	1 cut(s)	1632
FokI	GGATG	2 cut(s)	1411, 1739
FriOI	GRGCYC	1 cut(s)	134
FspBI	CTAG	4 cut(s)	768, 935, 1433, 1775
GlaI	GCGC	3 cut(s)	76, 773, 1058
GsaI	CCCAGC	1 cut(s)	1691
GsuI	CTGGAG	1 cut(s)	173
HaeIII	GGCC	3 cut(s)	126, 345, 900
HapII	CCGG	5 cut(s)	135, 192, 342, 346, 1552
HgaI	GACGC	2 cut(s)	197, 1739
HhaI	GCGC	3 cut(s)	77, 774, 1059
Hin1I	GRCGYC	1 cut(s)	208
Hin1II	CATG	7 cut(s)	45, 493, 708, 1289, 1429, 1591, 1747
Hin6I	GCGC	3 cut(s)	75, 772, 1057
HinP1I	GCGC	3 cut(s)	75, 772, 1057
HindIII	AAGCTT	1 cut(s)	1478
HinfI	GANTC	9 cut(s)	7, 85, 95, 202, 432, 721, 1232, 1385, 1541
HpaII	CCGG	5 cut(s)	135, 192, 342, 346, 1552
HphI	GGTGA	3 cut(s)	617, 941, 1079
Hpy166II	GTNNAC	5 cut(s)	25, 288, 406, 1585, 1633
Hpy188I	TCNGA	8 cut(s)	12, 322, 568, 838, 976, 1029, 1311, 1333
Hpy188III	TCNNGA	8 cut(s)	22, 192, 274, 587, 888, 1046, 1205, 1487
Hpy8I	GTNNAC	5 cut(s)	25, 288, 406, 1585, 1633
Hpy99I	CGWCG	4 cut(s)	148, 242, 1224, 1755
HpyAV	CCTTC	4 cut(s)	671, 843, 911, 1291
HpyCH4III	ACNGT	6 cut(s)	521, 858, 1220, 1462, 1637, 1710
HpyCH4IV	ACGT	1 cut(s)	563
HpySE526I	ACGT	1 cut(s)	563
Hsp92I	GRCGYC	1 cut(s)	208
Hsp92II	CATG	7 cut(s)	45, 493, 708, 1289, 1429, 1591, 1747
HspAI	GCGC	3 cut(s)	75, 772, 1057
Kpn2I	TCCGGA	1 cut(s)	191
LmnI	GCTCC	6 cut(s)	137, 194, 731, 778, 1207, 1300
Lsp1109I	GCAGC	9 cut(s)	213, 235, 286, 751, 1093, 1388, 1441, 1671, 1759
LweI	GCATC	6 cut(s)	393, 559, 823, 1257, 1267, 1798
MaeI	CTAG	4 cut(s)	768, 935, 1433, 1775
MaeII	ACGT	1 cut(s)	563
MaeIII	GTNAC	2 cut(s)	199, 1451
MboII	GAAGA	8 cut(s)	80, 258, 292, 416, 693, 1126, 1227, 1418
MflI	RGATCY	6 cut(s)	421, 553, 579, 595, 833, 1732
MhlI	GDGCHC	3 cut(s)	134, 1598, 1795
MluCI	AATT	9 cut(s)	195, 573, 891, 1097, 1124, 1164, 1197, 1334, 1466
MlyI	GAGTC	4 cut(s)	79, 89, 196, 426
MmeI	TCCRAC	3 cut(s)	1535, 1540, 1704
Mph1103I	ATGCAT	1 cut(s)	1250
MroI	TCCGGA	1 cut(s)	191
MroXI	GAANNNNTTC	2 cut(s)	284, 1152
MseI	TTAA	7 cut(s)	576, 1035, 1196, 1337, 1355, 1574, 1604
MslI	CAYNNNNRTG	2 cut(s)	1047, 1243
MspA1I	CMGCKG	2 cut(s)	226, 1106
MspI	CCGG	5 cut(s)	135, 192, 342, 346, 1552
MspR9I	CCNGG	3 cut(s)	429, 650, 897
Mva1269I	GAATGC	1 cut(s)	1378
MvaI	CCWGG	3 cut(s)	429, 650, 897
MvnI	CGCG	1 cut(s)	186
NcoI	CCATGG	1 cut(s)	704
NlaIII	CATG	7 cut(s)	45, 493, 708, 1289, 1429, 1591, 1747
NlaIV	GGNNCC	2 cut(s)	190, 1734
NmeAIII	GCCGAG	3 cut(s)	33, 184, 283
NmuCI	GTSAC	1 cut(s)	199
NsiI	ATGCAT	1 cut(s)	1250
NspI	RCATGY	1 cut(s)	45
NspV	TTCGAA	1 cut(s)	1156
PaeI	GCATGC	1 cut(s)	45
PceI	AGGCCT	1 cut(s)	900
PctI	GAATGC	1 cut(s)	1378
PdmI	GAANNNNTTC	2 cut(s)	284, 1152
PfeI	GAWTC	5 cut(s)	7, 721, 1232, 1385, 1541
PflMI	CCANNNNNTGG	1 cut(s)	710
PfoI	TCCNGGA	1 cut(s)	427
PleI	GAGTC	4 cut(s)	79, 89, 196, 426
PpsI	GAGTC	4 cut(s)	79, 89, 196, 426
PpuMI	RGGWCCY	1 cut(s)	1256
PshAI	GACNNNNGTC	1 cut(s)	1711
Psp124BI	GAGCTC	1 cut(s)	134
Psp5II	RGGWCCY	1 cut(s)	1256
Psp6I	CCWGG	3 cut(s)	427, 648, 895
PspEI	GGTNACC	1 cut(s)	1451
PspFI	CCCAGC	1 cut(s)	1687
PspGI	CCWGG	3 cut(s)	427, 648, 895
PspN4I	GGNNCC	2 cut(s)	190, 1734
PspPI	GGNCC	4 cut(s)	348, 646, 1256, 1821
PspPPI	RGGWCCY	1 cut(s)	1256
PstI	CTGCAG	1 cut(s)	1264
PsuI	RGATCY	6 cut(s)	421, 553, 579, 595, 833, 1732
PvuII	CAGCTG	2 cut(s)	226, 1106
RsaI	GTAC	7 cut(s)	655, 809, 855, 1018, 1644, 1756, 1784
RsaNI	GTAC	7 cut(s)	654, 808, 854, 1017, 1643, 1755, 1783
RseI	CAYNNNNRTG	2 cut(s)	1047, 1243
SacI	GAGCTC	1 cut(s)	134
SaqAI	TTAA	7 cut(s)	576, 1035, 1196, 1337, 1355, 1574, 1604
Sau96I	GGNCC	4 cut(s)	348, 646, 1256, 1821
SchI	GAGTC	4 cut(s)	79, 89, 196, 426
ScrFI	CCNGG	3 cut(s)	429, 650, 897
SduI	GDGCHC	3 cut(s)	134, 1598, 1795
SfaNI	GCATC	6 cut(s)	393, 559, 823, 1257, 1267, 1798
SfcI	CTRYAG	2 cut(s)	447, 1260
SfuI	TTCGAA	1 cut(s)	1156
SinI	GGWCC	4 cut(s)	348, 646, 1256, 1821
SmiMI	CAYNNNNRTG	2 cut(s)	1047, 1243
SmlI	CTYRAG	1 cut(s)	1597
SmoI	CTYRAG	1 cut(s)	1597
SphI	GCATGC	1 cut(s)	45
Sse9I	AATT	9 cut(s)	195, 573, 891, 1097, 1124, 1164, 1197, 1334, 1466
SseBI	AGGCCT	1 cut(s)	900
SsiI	CCGC	7 cut(s)	105, 123, 186, 212, 1268, 1474, 1555
SspI	AATATT	1 cut(s)	788
SspMI	CTAG	4 cut(s)	768, 935, 1433, 1775
SstI	GAGCTC	1 cut(s)	134
StuI	AGGCCT	1 cut(s)	900
StyD4I	CCNGG	3 cut(s)	427, 648, 895
StyI	CCWWGG	2 cut(s)	351, 704
TaaI	ACNGT	6 cut(s)	521, 858, 1220, 1462, 1637, 1710
TaiI	ACGT	1 cut(s)	566
TaqI	TCGA	7 cut(s)	237, 362, 666, 1023, 1156, 1527, 1668
TasI	AATT	9 cut(s)	195, 573, 891, 1097, 1124, 1164, 1197, 1334, 1466
TatI	WGTACW	2 cut(s)	807, 1782
TauI	GCSGC	3 cut(s)	126, 189, 1271
TfiI	GAWTC	5 cut(s)	7, 721, 1232, 1385, 1541
Tru1I	TTAA	7 cut(s)	576, 1035, 1196, 1337, 1355, 1574, 1604
Tru9I	TTAA	7 cut(s)	576, 1035, 1196, 1337, 1355, 1574, 1604
TscAI	CASTG	2 cut(s)	295, 1465
TseFI	GTSAC	1 cut(s)	199
TseI	GCWGC	9 cut(s)	223, 226, 299, 764, 1106, 1376, 1429, 1659, 1747
Tsp45I	GTSAC	1 cut(s)	199
TspDTI	ATGAA	4 cut(s)	493, 1227, 1274, 1415
TspRI	CASTG	2 cut(s)	295, 1465
Van91I	CCANNNNNTGG	1 cut(s)	710
VpaK11BI	GGWCC	4 cut(s)	348, 646, 1256, 1821
XagI	CCTNNNNNAGG	1 cut(s)	1209
XapI	RAATTY	2 cut(s)	891, 1466
XceI	RCATGY	1 cut(s)	45
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1357
XmiI	GTMKAC	1 cut(s)	1632
XmnI	GAANNNNTTC	2 cut(s)	284, 1152
XspI	CTAG	4 cut(s)	768, 935, 1433, 1775
Zsp2I	ATGCAT	1 cut(s)	1250

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.