Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001613

Physical Location & Seq

Genomic Coordinates Reverse (-)

19214783 .. 19221254

Gene Structure 6472 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1085600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGTTGGCTGTAGACTTGGTTTCAAATGCTGCATCTGCTCCGGCTTCAGAAATCATTTCTCAAATAGTGGAGGCCATTTTAGAAATTTCAGCTGCTGCCGGGGATGTCCTTGTTAAGAAGGAAACGTTTAAAGAGCTTTCAACTTATCTGGAAAGGATAGTCCCCATCTTAAAGGAGTTGAACAAGAAAGCTGTTCTTCATTCTGAGAGCTTAAGCAATGTTATGGCCATTCTTAACCGAGAAATCAGAGGTGCAAAGCAACTGACACTTGAGTGCAGGAAGAGAAACAAAGTGTATCTCTTAATGCATTGCCGAACAATAGTTAAGCGCCTAGGAGACACTATGAAAGAGATCAGTCGGGCTTTAGGTCTTCTTCCCCTGACTTCACTAGATCTTTCATCTGGCATAGTTGAAGAAATTGAAAAGCTTTGTGATAATATGCAGCGAGCTGAGTTCAGGGCAGCAATAGCAGAAGAAGAGATTTTGGACAAAATTGAGTCTGGAATCCAGGAGAGAAACGTTGATCGTGCTTATGCAAATAACTTGGTGGTTCTCATAGCAGGGGCAATCGGAATCTCAACTGAGAGGTCAGTCTTCAAGAAGGAATTAGAGGAATTCAGGAATGAGATAGAAAATGCTCGGCTGAGGAAAGACCAGGCTGAAGCTATACAGATGGAACAGATAATTGCTTTATTAGAAGGGGCTGATGCAGCTTCATCTTCTAAGGAGAAAGAAATGAAATATATGATTAAGCGGAATTCTTTAGGGTCTCAACCATTGGAACCACTTCAATCATTTTGTTGTCCAATCACCAGGGATGTTATGATAGACCCTGTGGAAACTTCTTCTGGACAGACATTTGAGAGAAGTGCTATAGAGAAGTGGTTTGCTGATGGGAACAAATCATGCCCCATGACCATGACTTCTTTGGACAGATCAATTTTGCGGCCCAATAAAACTCTCCGACAATCCATAGAAGAATGGAAGGATAGAAATACGATGATTATGATTGGTTCCTTGAAATCAAAACTCCAATCTGAAGAAGAAGAAGAAGTGCTTGATTGCCTGGGTGATTTACTAGATCTCTGTAAACAAAGAGAATTGCATAAGGAATGGATCATACTGGAGAACTACATACCTATTCTCATTCAACTTCTTGCTGTTAAAACTCCTGAGATAAGGAACAATGCTCTTCTCATCCTTTGCATTTTGGTGAAGGATAGTGATGATGCGAAGGAAAGAATTGCCATAGTTGATAATGGCATTGAATCTATTGTTCGTTCACTTGGGCGCCGTGTTGAGGAAAGGAAGTTAGCAGTTGCATTACTTTTGGAGTTATCAAAAAGTAATTTGATAAGAGAATGCATTGGGAAGGTTCAAGGCTGCATACTCCTATTAGTTACCATGTCCAACAGTGATGATAATCGGGCAGCCAGAGATGCACAAGAGCTACTGGAGAATCTGTCATTCTCTGATGAGAATGTTATACAGATGGCGAAGGCAAACTATTTTAAACATTTGCTGCAGCGTCTCACTACAGGACCGGAGGATGTAAAAACAGTCATGGCATCAAATTTGGCGGAGATGGAGTTGACCGACCACAATAAAGAATCTTTGGTTGAAGGAGGAGTAATGAGTCCGCTTCTTTACATGGTCTCACATGGTGACATTCCAATTAAAATGGTGGCTGTTAGAGCTCTTAGAAACCTCTCAAGCTTACCTAAAAATGGTCTACAGATGATTCGAGAAGGTGCAGAACGCCCATTACTTGACCTTCTTTTCAATCTTCGTCCGTCATCATCAAGTTTGCGTGAATATATAGCAGCCACGATTATGCAACTCTCCATGTCAATGGCGTCTCAAGAATCCAACCAGACACCCGTTTCATTTTTGGAATCAGATGAAGATATTGTCAAGCTTTTCTCTTTGATTAGCTTGATGGGGCCTAATGTACAGCAAAGCATCATTCGGACCTTCCACACCTTATGTCAATCCCCCTCTGCTACAAGTATCAAGAGCAAGTTGATGCAGTCCTCGGTTTTACAAGTATTGGTTCAGTTGTGCGAGAATGATGACCTAAACCTACGAGCAAATGCTGTAAAGCTGTTCTCTTGCTTAGTAGAACGCGGCAGTGAAGTCACCACTATCCTGGAGCATGTGAATCAGAAGTGCATTGAGACTATATTCAAGATCATCAAAACTTCTGATGACGAAGAAGAGATTGCTTCTGCAATGGGCATTATCTCTAACATTCCGGAAAACCCCGAGATCACACAGTGGCTTATGGATGATGGGGCACTACCTGTCATACTCAATTTTCTCCAGAATGGTTCCCATAGAAATCAGTTGATAGAGAATGCGGTCGGAGCCATTTGTCGTTTCACTGCTCCAACAAACTTGGAATGGCAAAAGAGTGCAGCTGAAGCTGGCATTATCCCATTGTTCGTACAATTGCTGGAGTCTGGAACCTCATTGACAAAGGAACGTGCAGCCATATCTCTTTCTCTATTTTCAAAGAGTTCACCACAGTTGAGCAGGTCTTCACCTAACCGCAAAGGATTCTGCTGCTTCTCGGCCCCACCAGAAACCAGATGCCCTGTTCATGGTGGAATCTGTGGCATTGTTTCATCATTTTGCCTTGTGGAGGCTGGTGCAGTGGGCCCACTGGTTAGGATTCTTGGGGAACCTGATCCTGGAGCTTGTGAGGCTTCTTTAGATGCACTATTAACTTTAATCGAAGGTGAGAGACTGCAGATGGGTAGTAAGGTGCTCACAGATGCGAATGCCATCCCACCTATAATAAAATTTCTTGTTCAACCATACCCTAGTTTGCAAGAGAAGGCCTTAAATGCTCTAGAAAGAATGTTCCGGCTGCTGGAGTTTAAACAGAAGTTTGGAGGCTCCGCTCAGATGCCTTTAGTCGACATAACTCAGCGTGGAAGCGGTAGTGTAAAATCTATGGCAGCCAGAATACTTGCTCACTTGAACGTGCTTCACGATCAGTCTTCTTATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm Arm_2 U-box

Protein Analysis

1006

Amino Acids

111.42

Weight (kDa)

5.41

Isoelectric Point (pI)

50.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	266 - 332	4e-18	U-box domain
ARM_ARMC5	PF24768	526 - 703	3.1e-09	ARMC5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001613)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g17110 FvH4_7g32250
malus_domestica	MD01G1085600.v1.1 MD07G1154800.v1.1 MD07G1295700.v1.1
prunus_persica	Prupe.2G193400_v2.0.a1 Prupe.2G315200_v2.0.a1 Prupe.2G315200_v2.0.a1 Prupe.2G315200_v2.0.a1 Prupe.2G315200_v2.0.a1 Prupe.2G315200_v2.0.a1 Prupe.2G315200_v2.0.a1
pyrus_communis	pycom07g15200 pycom07g26840
rosa_chinensis	RchiOBHm_Chr1g0360521 RchiOBHm_Chr1g0381931
rosa_laevigata	RLG00000026199 RLG00000027799
rosa_multiflora	Rmu_sc0010238.1_g000006 Rmu_ssc0000337.1_g000005
rosa_roxburghii	Rroxscaffold_4G00278330 Rroxscaffold_4G00296020
rosa_rugosa	Rorug01G0279400 Rorug01G0279500 Rorug01G0279600 Rorug01G0279700 Rorug01G0432300
rosa_samantha	Rh1AG291700 Rh1AG455500 Rh1BG256700 Rh1BG412700 Rh1CG274400 Rh1CG427500 Rh1DG286500 Rh1DG445000
rosa_wichuraiana	Rw1G025840 Rw1G041360 Rw1G041490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2526
AccB1I	GGYRCC	1 cut(s)	1290
AccBSI	CCGCTC	1 cut(s)	2906
AccI	GTMKAC	3 cut(s)	12, 1732, 2922
AccII	CGCG	1 cut(s)	2127
AccIII	TCCGGA	1 cut(s)	2254
AciI	CCGC	9 cut(s)	754, 946, 1580, 1640, 2127, 2360, 2551, 2904, 2943
AclI	AACGTT	2 cut(s)	125, 519
AclWI	GGATC	2 cut(s)	1124, 2684
AcoI	YGGCCR	1 cut(s)	225
AcsI	RAATTY	5 cut(s)	84, 614, 757, 1573, 2804
AcuI	CTGAAG	4 cut(s)	30, 681, 1059, 2442
AcyI	GRCGYC	2 cut(s)	1291, 1856
AdeI	CACNNNGTG	2 cut(s)	1664, 2277
AfaI	GTAC	2 cut(s)	1953, 2448
AfiI	CCNNNNNNNGG	6 cut(s)	1300, 1727, 2603, 2644, 2693, 2875
AflII	CTTAAG	1 cut(s)	211
AjnI	CCWGG	6 cut(s)	507, 654, 812, 1065, 2148, 2692
AleI	CACNNNNGTG	1 cut(s)	271
Alw21I	GWGCWC	2 cut(s)	1699, 2772
Alw26I	GTCTC	7 cut(s)	330, 774, 1535, 1660, 1863, 2171, 2740
AlwI	GGATC	2 cut(s)	1124, 2684
AlwNI	CAGNNNCTG	1 cut(s)	95
Ama87I	CYCGRG	1 cut(s)	2264
Aor13HI	TCCGGA	1 cut(s)	2254
AoxI	GGCC	7 cut(s)	72, 225, 947, 1943, 2574, 2659, 2841
ApaI	GGGCCC	1 cut(s)	2663
ApoI	RAATTY	5 cut(s)	84, 614, 757, 1573, 2804
Asp700I	GAANNNNTTC	1 cut(s)	786
AspA2I	CCTAGG	1 cut(s)	331
AspLEI	GCGC	2 cut(s)	330, 1293
AspS9I	GGNCC	7 cut(s)	948, 1541, 1943, 1971, 2575, 2659, 2660
AsuC2I	CCSGG	1 cut(s)	100
AsuHPI	GGTGA	8 cut(s)	802, 1082, 1225, 1676, 2131, 2514, 2535, 2753
AvaI	CYCGRG	1 cut(s)	2264
AvaII	GGWCC	2 cut(s)	1541, 1971
AvrII	CCTAGG	1 cut(s)	331
BaeGI	GKGCMC	2 cut(s)	2299, 2663
BalI	TGGCCA	1 cut(s)	227
BanI	GGYRCC	1 cut(s)	1290
BanII	GRGCYC	2 cut(s)	1699, 2663
BbsI	GAAGAC	4 cut(s)	362, 586, 2532, 2997
Bbv12I	GWGCWC	2 cut(s)	1699, 2772
BbvCI	CCTCAGC	1 cut(s)	644
BccI	CCATC	9 cut(s)	173, 667, 887, 1486, 1579, 1933, 2285, 2749, 2795
BceAI	ACGGC	1 cut(s)	1278
BciT130I	CCWGG	6 cut(s)	509, 656, 814, 1067, 2150, 2694
BcnI	CCSGG	1 cut(s)	100
BcoDI	GTCTC	7 cut(s)	330, 774, 1535, 1660, 1863, 2171, 2740
BfaI	CTAG	5 cut(s)	332, 389, 1079, 2826, 2855
BfmI	CTRYAG	6 cut(s)	9, 873, 1523, 1536, 1733, 2750
BfoI	RGCGCY	2 cut(s)	331, 1294
BfrI	CTTAAG	1 cut(s)	211
BfuAI	ACCTGC	1 cut(s)	2526
BglII	AGATCT	2 cut(s)	391, 1081
BlnI	CCTAGG	1 cut(s)	331
Bme1390I	CCNGG	7 cut(s)	100, 509, 656, 814, 1067, 2150, 2694
Bme18I	GGWCC	2 cut(s)	1541, 1971
BmeT110I	CYCGRG	1 cut(s)	2264
BmgT120I	GGNCC	7 cut(s)	948, 1541, 1943, 1971, 2575, 2659, 2660
BmrFI	CCNGG	7 cut(s)	100, 509, 656, 814, 1067, 2150, 2694
BpiI	GAAGAC	4 cut(s)	362, 586, 2532, 2997
BpmI	CTGGAG	7 cut(s)	1145, 1475, 2171, 2306, 2477, 2715, 2897
Bpu10I	CCTNAGC	1 cut(s)	644
BpuEI	CTTGAG	3 cut(s)	290, 1696, 1845
BpuMI	CCSGG	1 cut(s)	100
BsaBI	GATNNNNATC	1 cut(s)	1422
BsaHI	GRCGYC	2 cut(s)	1291, 1856
BsaI	GGTCTC	2 cut(s)	774, 1660
BsaJI	CCNNGG	5 cut(s)	99, 331, 813, 1066, 2034
BsaWI	WCCGGW	2 cut(s)	1543, 2254
BsaXI	ACNNNNNCTCC	4 cut(s)	1448, 1478, 1574, 1604
Bsc4I	CCNNNNNNNGG	6 cut(s)	1300, 1727, 2603, 2644, 2693, 2875
Bse1I	ACTGG	3 cut(s)	1128, 1458, 2670
Bse3DI	GCAATG	3 cut(s)	223, 307, 2238
Bse8I	GATNNNNATC	1 cut(s)	1422
BseAI	TCCGGA	1 cut(s)	2254
BseBI	CCWGG	6 cut(s)	509, 656, 814, 1067, 2150, 2694
BseDI	CCNNGG	5 cut(s)	99, 331, 813, 1066, 2034
BseGI	GGATG	6 cut(s)	109, 823, 1197, 1555, 2293, 2787
BseJI	GATNNNNATC	1 cut(s)	1422
BseLI	CCNNNNNNNGG	6 cut(s)	1300, 1727, 2603, 2644, 2693, 2875
BseMI	GCAATG	3 cut(s)	223, 307, 2238
BseMII	CTCAG	7 cut(s)	195, 441, 573, 635, 1164, 2921, 2945
BseNI	ACTGG	3 cut(s)	1128, 1458, 2670
BseRI	GAGGAG	1 cut(s)	1641
BseSI	GKGCMC	2 cut(s)	2299, 2663
BsgI	GTGCAG	5 cut(s)	295, 1773, 2436, 2508, 2673
Bsh1236I	CGCG	1 cut(s)	2127
Bsh1285I	CGRYCG	1 cut(s)	2364
BshFI	GGCC	7 cut(s)	74, 227, 949, 1945, 2576, 2661, 2843
BshNI	GGYRCC	1 cut(s)	1290
BsiEI	CGRYCG	1 cut(s)	2364
BsiHKAI	GWGCWC	2 cut(s)	1699, 2772
BsiHKCI	CYCGRG	1 cut(s)	2264
BsiSI	CCGG	5 cut(s)	41, 99, 1544, 2255, 2869
BslFI	GGGAC	1 cut(s)	146
BslI	CCNNNNNNNGG	6 cut(s)	1300, 1727, 2603, 2644, 2693, 2875
BsmAI	GTCTC	7 cut(s)	330, 774, 1535, 1660, 1863, 2171, 2740
BsmBI	CGTCTC	2 cut(s)	1535, 1863
BsmFI	GGGAC	1 cut(s)	146
BsmI	GAATGC	3 cut(s)	1367, 2362, 2788
BsnI	GGCC	7 cut(s)	74, 227, 949, 1945, 2576, 2661, 2843
Bso31I	GGTCTC	2 cut(s)	774, 1660
BsoBI	CYCGRG	1 cut(s)	2264
Bsp120I	GGGCCC	1 cut(s)	2659
Bsp1286I	GDGCHC	4 cut(s)	1699, 2299, 2663, 2772
Bsp13I	TCCGGA	1 cut(s)	2254
Bsp1407I	TGTACA	1 cut(s)	1951
BspACI	CCGC	9 cut(s)	754, 946, 1580, 1640, 2127, 2360, 2551, 2904, 2943
BspANI	GGCC	7 cut(s)	74, 227, 949, 1945, 2576, 2661, 2843
BspCNI	CTCAG	7 cut(s)	196, 442, 574, 636, 1165, 2920, 2944
BspEI	TCCGGA	1 cut(s)	2254
BspFNI	CGCG	1 cut(s)	2127
BspMAI	CTGCAG	2 cut(s)	1527, 2754
BspMI	ACCTGC	1 cut(s)	2526
BspPI	GGATC	2 cut(s)	1124, 2684
BspQI	GCTCTTC	1 cut(s)	1197
BspT107I	GGYRCC	1 cut(s)	1290
BspTI	CTTAAG	1 cut(s)	211
BspTNI	GGTCTC	2 cut(s)	774, 1660
BsrBI	CCGCTC	1 cut(s)	2906
BsrDI	GCAATG	3 cut(s)	223, 307, 2238
BsrGI	TGTACA	1 cut(s)	1951
BsrI	ACTGG	3 cut(s)	1128, 1458, 2670
BssECI	CCNNGG	5 cut(s)	99, 331, 813, 1066, 2034
BssNI	GRCGYC	2 cut(s)	1291, 1856
BssT1I	CCWWGG	1 cut(s)	331
Bst2UI	CCWGG	6 cut(s)	509, 656, 814, 1067, 2150, 2694
Bst4CI	ACNGT	4 cut(s)	1415, 1561, 2277, 2529
Bst6I	CTCTTC	4 cut(s)	275, 471, 1197, 2211
BstACI	GRCGYC	2 cut(s)	1291, 1856
BstAFI	CTTAAG	1 cut(s)	211
BstAUI	TGTACA	1 cut(s)	1951
BstC8I	GCNNGC	2 cut(s)	447, 2428
BstENI	CCTNNNNNAGG	1 cut(s)	2642
BstF5I	GGATG	6 cut(s)	109, 823, 1197, 1555, 2293, 2787
BstFNI	CGCG	1 cut(s)	2127
BstH2I	RGCGCY	2 cut(s)	331, 1294
BstHHI	GCGC	2 cut(s)	330, 1293
BstMAI	GTCTC	7 cut(s)	330, 774, 1535, 1660, 1863, 2171, 2740
BstMCI	CGRYCG	1 cut(s)	2364
BstMWI	GCNNNNNNNGC	9 cut(s)	35, 467, 710, 1439, 1694, 2366, 2423, 2705, 2849
BstNI	CCWGG	6 cut(s)	509, 656, 814, 1067, 2150, 2694
BstNSI	RCATGY	1 cut(s)	2159
BstSCI	CCNGG	7 cut(s)	98, 507, 654, 812, 1065, 2148, 2692
BstSFI	CTRYAG	6 cut(s)	9, 873, 1523, 1536, 1733, 2750
BstSLI	GKGCMC	2 cut(s)	2299, 2663
BstUI	CGCG	1 cut(s)	2127
BstV2I	GAAGAC	4 cut(s)	362, 586, 2532, 2997
BstX2I	RGATCY	2 cut(s)	391, 1081
BstXI	CCANNNNNNTGG	2 cut(s)	1852, 2149
BstYI	RGATCY	2 cut(s)	391, 1081
BsuRI	GGCC	7 cut(s)	74, 227, 949, 1945, 2576, 2661, 2843
BtsCI	GGATG	6 cut(s)	109, 823, 1197, 1555, 2293, 2787
BtsI	GCAGTG	3 cut(s)	2137, 2382, 2661
BtsIMutI	CAGTG	6 cut(s)	1420, 2137, 2282, 2382, 2661, 2663
BveI	ACCTGC	1 cut(s)	2526
Cac8I	GCNNGC	2 cut(s)	447, 2428
CaiI	CAGNNNCTG	1 cut(s)	95
CfoI	GCGC	2 cut(s)	330, 1293
Cfr13I	GGNCC	7 cut(s)	948, 1541, 1943, 1971, 2575, 2659, 2660
CseI	GACGC	2 cut(s)	1517, 1845
Csp6I	GTAC	2 cut(s)	1952, 2447
CviQI	GTAC	2 cut(s)	1952, 2447
DinI	GGCGCC	1 cut(s)	1292
DraI	TTTAAA	3 cut(s)	130, 1513, 2884
DraIII	CACNNNGTG	2 cut(s)	1664, 2277
EaeI	YGGCCR	1 cut(s)	225
Eam1104I	CTCTTC	4 cut(s)	275, 471, 1197, 2211
EarI	CTCTTC	4 cut(s)	275, 471, 1197, 2211
EciI	GGCGGA	1 cut(s)	1595
Ecl136II	GAGCTC	1 cut(s)	1697
Eco130I	CCWWGG	1 cut(s)	331
Eco147I	AGGCCT	1 cut(s)	2843
Eco24I	GRGCYC	2 cut(s)	1699, 2663
Eco31I	GGTCTC	2 cut(s)	774, 1660
Eco47I	GGWCC	2 cut(s)	1541, 1971
Eco53kI	GAGCTC	1 cut(s)	1697
Eco57I	CTGAAG	4 cut(s)	30, 681, 1059, 2442
Eco88I	CYCGRG	1 cut(s)	2264
EcoICRI	GAGCTC	1 cut(s)	1697
EcoNI	CCTNNNNNAGG	1 cut(s)	2642
EcoO109I	RGGNCCY	1 cut(s)	1943
EcoRI	GAATTC	2 cut(s)	614, 757
EcoRII	CCWGG	6 cut(s)	507, 654, 812, 1065, 2148, 2692
EcoT14I	CCWWGG	1 cut(s)	331
EcoT22I	ATGCAT	2 cut(s)	309, 1367
EcoT38I	GRGCYC	2 cut(s)	1699, 2663
EgeI	GGCGCC	1 cut(s)	1292
EheI	GGCGCC	1 cut(s)	1292
ErhI	CCWWGG	1 cut(s)	331
Esp3I	CGTCTC	2 cut(s)	1535, 1863
FalI	AAGNNNNNCTT	4 cut(s)	180, 212, 1041, 1073
FaqI	GGGAC	1 cut(s)	146
FblI	GTMKAC	3 cut(s)	12, 1732, 2922
FokI	GGATG	6 cut(s)	116, 830, 1184, 1562, 2300, 2774
FriOI	GRGCYC	2 cut(s)	1699, 2663
FspBI	CTAG	5 cut(s)	332, 389, 1079, 2826, 2855
GlaI	GCGC	2 cut(s)	329, 1292
GsuI	CTGGAG	7 cut(s)	1145, 1475, 2171, 2306, 2477, 2715, 2897
HaeII	RGCGCY	2 cut(s)	331, 1294
HaeIII	GGCC	7 cut(s)	74, 227, 949, 1945, 2576, 2661, 2843
HapII	CCGG	5 cut(s)	41, 99, 1544, 2255, 2869
HgaI	GACGC	2 cut(s)	1517, 1845
HhaI	GCGC	2 cut(s)	330, 1293
Hin1I	GRCGYC	2 cut(s)	1291, 1856
Hin6I	GCGC	2 cut(s)	328, 1291
HinP1I	GCGC	2 cut(s)	328, 1291
HincII	GTYRAC	2 cut(s)	1593, 2923
HindII	GTYRAC	2 cut(s)	1593, 2923
HindIII	AAGCTT	3 cut(s)	425, 1714, 1916
HpaII	CCGG	5 cut(s)	41, 99, 1544, 2255, 2869
HphI	GGTGA	8 cut(s)	802, 1082, 1225, 1676, 2131, 2514, 2535, 2753
Hpy166II	GTNNAC	7 cut(s)	13, 1091, 1283, 1593, 1733, 2522, 2923
Hpy8I	GTNNAC	7 cut(s)	13, 1091, 1283, 1593, 1733, 2522, 2923
HpyCH4III	ACNGT	4 cut(s)	1415, 1561, 2277, 2529
HpyCH4IV	ACGT	4 cut(s)	125, 519, 2485, 2988
HpyF10VI	GCNNNNNNNGC	9 cut(s)	35, 467, 710, 1439, 1694, 2366, 2423, 2705, 2849
HpySE526I	ACGT	4 cut(s)	125, 519, 2485, 2988
Hsp92I	GRCGYC	2 cut(s)	1291, 1856
HspAI	GCGC	2 cut(s)	328, 1291
KasI	GGCGCC	1 cut(s)	1290
Kpn2I	TCCGGA	1 cut(s)	2254
LguI	GCTCTTC	1 cut(s)	1197
LmnI	GCTCC	6 cut(s)	43, 2152, 2366, 2392, 2696, 2906
MaeI	CTAG	5 cut(s)	332, 389, 1079, 2826, 2855
MaeII	ACGT	4 cut(s)	125, 519, 2485, 2988
MaeIII	GTNAC	3 cut(s)	1399, 1664, 2137
MbiI	CCGCTC	1 cut(s)	2906
MfeI	CAATTG	1 cut(s)	2450
MflI	RGATCY	2 cut(s)	391, 1081
MhlI	GDGCHC	4 cut(s)	1699, 2299, 2663, 2772
MlsI	TGGCCA	1 cut(s)	227
MluNI	TGGCCA	1 cut(s)	227
Mly113I	GGCGCC	1 cut(s)	1291
MlyI	GAGTC	3 cut(s)	506, 1645, 2468
MmeI	TCCRAC	5 cut(s)	988, 1434, 1893, 2344, 2414
Mox20I	TGGCCA	1 cut(s)	227
Mph1103I	ATGCAT	2 cut(s)	309, 1367
MroI	TCCGGA	1 cut(s)	2254
MroXI	GAANNNNTTC	1 cut(s)	786
MscI	TGGCCA	1 cut(s)	227
MslI	CAYNNNNRTG	5 cut(s)	271, 917, 1211, 1832, 1850
Msp20I	TGGCCA	1 cut(s)	227
MspA1I	CMGCKG	2 cut(s)	92, 2420
MspCI	CTTAAG	1 cut(s)	211
MspI	CCGG	5 cut(s)	41, 99, 1544, 2255, 2869
MspR9I	CCNGG	7 cut(s)	100, 509, 656, 814, 1067, 2150, 2694
MssI	GTTTAAAC	1 cut(s)	2884
MunI	CAATTG	1 cut(s)	2450
Mva1269I	GAATGC	3 cut(s)	1367, 2362, 2788
MvaI	CCWGG	6 cut(s)	509, 656, 814, 1067, 2150, 2694
MvnI	CGCG	1 cut(s)	2127
MwoI	GCNNNNNNNGC	9 cut(s)	35, 467, 710, 1439, 1694, 2366, 2423, 2705, 2849
NarI	GGCGCC	1 cut(s)	1291
NciI	CCSGG	1 cut(s)	100
NmeAIII	GCCGAG	2 cut(s)	619, 2552
NmuCI	GTSAC	2 cut(s)	1664, 2137
NsiI	ATGCAT	2 cut(s)	309, 1367
NspI	RCATGY	1 cut(s)	2159
OliI	CACNNNNGTG	1 cut(s)	271
PceI	AGGCCT	1 cut(s)	2843
PciSI	GCTCTTC	1 cut(s)	1197
PcsI	WCGNNNNNNNCGW	1 cut(s)	2994
PctI	GAATGC	3 cut(s)	1367, 2362, 2788
PdmI	GAANNNNTTC	1 cut(s)	786
PfoI	TCCNGGA	3 cut(s)	507, 2148, 2692
PleI	GAGTC	3 cut(s)	505, 1644, 2467
PluTI	GGCGCC	1 cut(s)	1294
PmeI	GTTTAAAC	1 cut(s)	2884
PpsI	GAGTC	3 cut(s)	505, 1644, 2467
Psp124BI	GAGCTC	1 cut(s)	1699
Psp1406I	AACGTT	2 cut(s)	125, 519
Psp6I	CCWGG	6 cut(s)	507, 654, 812, 1065, 2148, 2692
PspGI	CCWGG	6 cut(s)	507, 654, 812, 1065, 2148, 2692
PspOMI	GGGCCC	1 cut(s)	2659
PspPI	GGNCC	7 cut(s)	948, 1541, 1943, 1971, 2575, 2659, 2660
PsrI	GAACNNNNNNTAC	2 cut(s)	1749, 1781
PstI	CTGCAG	2 cut(s)	1527, 2754
PstNI	CAGNNNCTG	1 cut(s)	95
PsuI	RGATCY	2 cut(s)	391, 1081
PvuII	CAGCTG	2 cut(s)	92, 2420
RsaI	GTAC	2 cut(s)	1953, 2448
RsaNI	GTAC	2 cut(s)	1952, 2447
RseI	CAYNNNNRTG	5 cut(s)	271, 917, 1211, 1832, 1850
SacI	GAGCTC	1 cut(s)	1699
SalI	GTCGAC	1 cut(s)	2921
SapI	GCTCTTC	1 cut(s)	1197
Sau96I	GGNCC	7 cut(s)	948, 1541, 1943, 1971, 2575, 2659, 2660
SchI	GAGTC	3 cut(s)	506, 1645, 2468
ScrFI	CCNGG	7 cut(s)	100, 509, 656, 814, 1067, 2150, 2694
SduI	GDGCHC	4 cut(s)	1699, 2299, 2663, 2772
SfcI	CTRYAG	6 cut(s)	9, 873, 1523, 1536, 1733, 2750
SfoI	GGCGCC	1 cut(s)	1292
SinI	GGWCC	2 cut(s)	1541, 1971
SmiMI	CAYNNNNRTG	5 cut(s)	271, 917, 1211, 1832, 1850
SmlI	CTYRAG	4 cut(s)	211, 269, 1711, 1860
SmoI	CTYRAG	4 cut(s)	211, 269, 1711, 1860
SseBI	AGGCCT	1 cut(s)	2843
SsiI	CCGC	9 cut(s)	754, 946, 1580, 1640, 2127, 2360, 2551, 2904, 2943
SspDI	GGCGCC	1 cut(s)	1290
SspMI	CTAG	5 cut(s)	332, 389, 1079, 2826, 2855
SstI	GAGCTC	1 cut(s)	1699
StuI	AGGCCT	1 cut(s)	2843
StyD4I	CCNGG	7 cut(s)	98, 507, 654, 812, 1065, 2148, 2692
StyI	CCWWGG	1 cut(s)	331
TaaI	ACNGT	4 cut(s)	1415, 1561, 2277, 2529
TaiI	ACGT	4 cut(s)	128, 522, 2488, 2991
TaqI	TCGA	3 cut(s)	1744, 2736, 2922
TaqII	GACCGA	1 cut(s)	1610
TatI	WGTACW	1 cut(s)	1951
TauI	GCSGC	2 cut(s)	949, 2130
TscAI	CASTG	6 cut(s)	1420, 2137, 2282, 2389, 2661, 2670
TseFI	GTSAC	2 cut(s)	1664, 2137
Tsp45I	GTSAC	2 cut(s)	1664, 2137
TspDTI	ATGAA	9 cut(s)	188, 359, 387, 705, 752, 1875, 1917, 2591, 2616
TspGWI	ACGGA	1 cut(s)	1782
TspRI	CASTG	6 cut(s)	1420, 2137, 2282, 2389, 2661, 2670
Vha464I	CTTAAG	1 cut(s)	211
VpaK11BI	GGWCC	2 cut(s)	1541, 1971
XagI	CCTNNNNNAGG	1 cut(s)	2642
XapI	RAATTY	5 cut(s)	84, 614, 757, 1573, 2804
XbaI	TCTAGA	1 cut(s)	2854
XceI	RCATGY	1 cut(s)	2159
XcmI	CCANNNNNNNNNTGG	1 cut(s)	925
XmaJI	CCTAGG	1 cut(s)	331
XmiI	GTMKAC	3 cut(s)	12, 1732, 2922
XmnI	GAANNNNTTC	1 cut(s)	786
XspI	CTAG	5 cut(s)	332, 389, 1079, 2826, 2855
Zsp2I	ATGCAT	2 cut(s)	309, 1367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.