Rorug01G0279700

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001613
Physical Location & Seq
Reverse (-)
39263462 .. 39267883
4422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0279700.1

Sequence Viewer

Length: 4422 bp
ATGGAGTTGGGAAGGTGGCTCAAACATGCTTATGCAATATCAATTGTGCTTCTTCTGCATATCAACGCTTCCTTATCCCTTGGCCACCACCATGCTAACGTGACGGCAAGGTGCCTAGAGAGGGAGAGGGATGCACTGCTTGCTATCAAAGGAGACCTGGTGGATGAATACAACCTGCTCTCTTCGTGGGGAAGTGAAGCCAAAAAGCGAGACTGTTGCGGATGGGAAAGAGTCCACTGCGATCACCAAACTGGCCATGTTACTCAACTTCATCTTGGAGGACTGAAGGTGATCTCTTCATCAGGTGAGTTTATGACTTACAATTATTTTAAAGGAAAAGTTAGTCCTAAACTCATTGAGTTGCAGCATTTGGAATATTTGGACCTCAGTTACAATTTCTTCAATGCCAGCCAAATTCCAGCGCTCATTTATTCTCTGTCCAATTTAAGACACCTAGATCTCCATTACAATTACCTCACTAATGGGAGCAAAATTCTAGAGCTCATTGGTAACCTGACCAATTTAAGATATCTAGATCTCTATGGGAATGGCCTAGGTGATGAAGTTCCATATCATCAGCTGAGAAACCTTACTCACTTGCAGTATCTCAATCTCGGGTATAATGACATCACTCATGGTGTAGAAAACCTCCATTGGCTCCCACATCTTTCATCTTTAAAATACCTGGACCTGAGTTTTACCAATCTAAGTAAGGTTTCTGATTGGCTGGAAACAATAAACAAGCTCCCCAATTTAAGAAACTTGACCTTGAGCTTCTGTATTCTTCCTCCTCCAACTCTTTCCGCTCTTTCTCACATAAATTCTCCCAATTCTCTTGTTAGTGTTGATTTGTCTCAAAATGATCCTGTTGTCATTTCTTCAATATTCAAATGGTTGTGCAATTTCAATACCACCCTTGTTCATCTTGACCTCTTTGACAGCCAATTAAATGGTTCGATTCCCAATGCTTTCAGAAACATGGGCTCACTTGAATATCTTGACCTCTCTTCCAACCAATTTAGCGGTTCGATTCCCGATGCTTTCAGAAACCTTGGCTCACTTGAATATCTTGACCTCTCTTCCAACCAATTTAGTGGTTTGATTCCCGATGCTTTCAGAAACATGGGCTCACTTGCAAGTCTTGACCTCTCTTCCAACCAATTAAGTGGTTCGATTCCTGATGCTTTCAGAAACATGAGCTCACTTGCAAGTCTTGACCTCTCTTCCAACCAATTAAGTGGTTCGATTCCTGATGCTTTCAGAAACATGAGCTCACTTGTAAGTCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCTATGCTTTCGCAAACATGGGCTCACTTGCAAGTCTTCGCCTCTATTCCAACCAATTAAGTGGTTTGATTCCCAATGCTTTCGCAAACATGGGCTCACTTGCAAGTCTTCCCCTCTATTCCAACCAATTAAGTGGTTTGACTCCCGATGCTTTCAGAAACATGAGCTCACTTGCAAGTCTTGACCTCTCTTCCAACCAATTAAGTGGTTCGATTCCTGATGCTTTCAGAAACATGAGCTCACTTGTAAGTCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGAGCTCACTTGCATGTCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCTATGCTTTCGCAAACATGGGCTCACTTGCAAGTCTTCGCCTCTATTCCAACCAATTAAGTGGTTTGATTCCCAATGCTTTCGCAAACATGGGCTCACTTGCAAGTCTTCCCCTCTATTCCAACCAATTAAGTGGTTTGACTCCCGATGCTTTCAGAAACATGAGCTCACTTGCAAGTCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCTTATGCTTTTGCAAACATGAGCTCACTTGCAACTCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCTTATGCTTTTGCAAACATGAGCTTACTTGCAACTCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAACATGAACTCACTTGCAAGTCTTGACCTCTCTTCCAACCAATTTAGTGGTTTGATTCCCGATGCTTTCAGAAACATGGGCTCACTTATTCATCTATACCTCTTTTCCAACCAATTAAGTGGTTTGACTCCGAATGCTTTCGCAAACATGAGCTCACTTGTAGACCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGATTCCCGATGCTTTCAGAAATATGGGCTCACTTGAATATCTTGACCTCTCTTCCAACCAATTAAGTGGTTTGACTCCCGATGCTTTCAGAAACATGGGCTCACTTGAATATCTATACCTGTCTTCCAACCAATTAAATGGTTTGATTCCCAATGCTTTCGCAAACATGAGCTCACTTTCATATCTTAGCCTCTCTTCCAACCAATTAAGTGGTTTGATTCACGATGGTTTTGCAAACATGAGCTCACTTGCAAGTCTTGACATCTCTTATAACCCATTCGGAGGAGGGATCCCAGCATCCATTGGCCAGTTATGCACTTTGCGACATGTGTCAGTTTTAGGTTGTAATCTGACAAGTGGACAACTTTCTAAGTTTGTTCAAGGATGTTCAGCATGTGTTCAAAACTCATTACAGAGTTTTGATCTTTCCAATAATCATCTTGCTGGGTCACTTCCTAATCTTACAAGCTTTTCATCATTGGAGGTATTAACTCTCTCCAAAAATAAGTTGAGTGTTCTGGATAGTATTGGAAATTTTTCTTCTCTGAAAAAATTATATGTTGATCAAAATCAATTACGATGGATGATACCGGAAAGTATTGGGAAACTCTCCGGTTTGTATGTCTTGGATCTCTCTGGAAATCTTCTGAGCGGAAGAATTCCAGAAGGTATTGGGCAACTCTCATCATTGACAGAATTAATTCTTTCTGGAAATCAATTAAACAGAAGCATACCTGAAACTATTGGGCAACTATCATCTTTGCGAAGTTTAGATCTCTCTGGAAATCTGTTAAGCGGAAGAATACCGAAAAGTATTGGACAGTTGTCGAGGTTGTTCCATATAAATCTTAGTATGAATCATTTGGCGGGGGTGATTTCGGAAACTCATTTCTCAAAACTCTTCGATGATCATTTAGGTGAGTTAGATTTATCCTATAACTCATTATTAGAATTCAATGTCACTTCTGATTGGGTTCCACCTTTTCAATTGTCTTATCTAAGTTTGAGGTCTTGTATGGTAGGGCCGGATTTCCCAAAATGGCTTCGAACCCAAAAATATCTTTCCTACCTTGACATGTCTGATGGTGACATTTCAGATGTCCTTCCAAGTTGGTTCTGGGGTCTATTGCGTGACGGTTCGACTATAGATCTGTCTAACAATCAAATTAGAGGAACATTAGGGAGTTCAGAGGCTGTATCATTCAGCAGAGTTGAAGTTCGATTTAGTTCGAACCAGTTGGAAGGTCCAATACCCTCATTTCTATCAAAAGCGTCATATTTGGATCTCTCTGATAATAAATTTTCAGAGGTGACTTCCTTCTTGTGTTCTGCGAAAGTTAAAATTTCCAAATTTCTTGATCTCTCCAGTAACCATATTCCTGGAGAACTTCCAGATTGCTGGAGACATTGGGTCAATCTAGAACTTCTCGATTTGAGTAACAATTCTTTTTCTGGGAAAATCCCACCCAATATAGGATGTTTACTTCGAATCAAAACATTGAAATTAAGGAGCAACAGACTTGTGGGAGAATTGCCTTCATCCTTGAAGAATTGTACAAGCTTAAATGTTATTGATCTTGGGAACAATAAGCTTTCAAGTTCAGTGCCTGAATGGTTAGGGGTCAGTCTTCCAAATTTGGTTATTCTTATGCTTCAATCTAATCAGCTTAGTGGAAGTTTGCCTTCACAATTATGCCATCTAGCACGCCTTCAAATTTTGGATGTCTCTGTGAATCAAATCTCTGGATCTATACCAAAATGCCTCGATAATTTGACTTCTTTGGCGCAAGAAGGAAATTCAAATCTAACCATCAGACATTCTTTTAATACATCTGAGGCCGAACCACCTATGAGTTCTCTTTATTATGAGGATGATGCAACCTTCATGTGGAAGAGAACATTGTCTACATACAAAAACACTTTGGGGCTAGTCAAGAGCATTGATCTCTCATGCAACAGATTAACTGGGGAGATTCCAAGTGAAATCACTCATCTTGTTGGGCTCGTTTCTCTGAACCTTTCAAGGAACAATTTGACTGGACAAATACCTCTAGAGATCGGAAAGTTGAAGTCACTAGATTCTCTTGATTTGTCTAGGAACAAGATTGGTGGCAGGATTCCGACAAGCCTTGCTCGGATTGATAGACTTGGTGACATGGATTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_2 U-box

Protein Analysis

1473

Amino Acids

160.82

Weight (kDa)

5.65

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 81 1.1e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 114 - 259 2.9e-10 Leucine-rich repeat region
LRR_8 PF13855 305 - 364 7.8e-11 Leucine rich repeat
LRR_8 PF13855 345 - 388 1.4e-10 Leucine rich repeat
LRR_8 PF13855 361 - 412 2.1e-10 Leucine rich repeat
LRR_8 PF13855 377 - 436 7.7e-13 Leucine rich repeat
LRR_8 PF13855 402 - 460 5.3e-11 Leucine rich repeat
LRR_5 PF13306 418 - 546 4.6e-09 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 429 - 484 1.4e-08 Leucine rich repeat
LRR_8 PF13855 449 - 508 2.6e-11 Leucine rich repeat
LRR_8 PF13855 473 - 532 2e-13 Leucine rich repeat
LRR_8 PF13855 513 - 556 5.9e-10 Leucine rich repeat
LRR_5 PF13306 514 - 618 8.6e-07 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 549 - 604 1.2e-08 Leucine rich repeat
LRR_5 PF13306 586 - 711 7.4e-10 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 593 - 652 4.4e-14 Leucine rich repeat
LRR_8 PF13855 641 - 700 7.8e-14 Leucine rich repeat
LRR_5 PF13306 658 - 770 2.7e-07 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 689 - 748 1.2e-12 Leucine rich repeat
LRR_5 PF13306 729 - 840 1.1e-07 BspA type Leucine rich repeat region (6 copies)
LRR_8 PF13855 737 - 796 9.4e-17 Leucine rich repeat
LRR_8 PF13855 793 - 843 1.3e-08 Leucine rich repeat
LRR_14 PF23598 900 - 1110 2.9e-19 Leucine-rich repeat region
LRR_8 PF13855 932 - 991 1e-08 Leucine rich repeat
LRR_8 PF13855 1248 - 1307 4.2e-06 Leucine rich repeat
LRR_8 PF13855 1395 - 1453 1.3e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2523
Acc36I ACCTGC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 111
AccBSI CCGCTC 2 cut(s) 806, 2904
AccI GTMKAC 2 cut(s) 2217, 4157
AciI CCGC 6 cut(s) 219, 804, 1023, 2904, 3048, 3119
AclWI GGATC 6 cut(s) 857, 2536, 2549, 2889, 3543, 4006
AcoI YGGCCR 3 cut(s) 82, 253, 2557
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 1 cut(s) 3808
AfeI AGCGCT 1 cut(s) 423
AfiI CCNNNNNNNGG 4 cut(s) 121, 2534, 3725, 4140
AflIII ACRYGT 2 cut(s) 2578, 3327
AhdI GACNNNNNGTC 1 cut(s) 3662
AjnI CCWGG 3 cut(s) 156, 684, 3631
Alw26I GTCTC 5 cut(s) 147, 204, 858, 3649, 3982
AlwI GGATC 6 cut(s) 857, 2536, 2549, 2889, 3543, 4006
AlwNI CAGNNNCTG 2 cut(s) 3446, 3860
Ama87I CYCGRG 1 cut(s) 614
Aor51HI AGCGCT 1 cut(s) 423
AoxI GGCC 6 cut(s) 82, 253, 550, 2557, 3275, 4089
ApeKI GCWGC 1 cut(s) 364
AseI ATTAAT 1 cut(s) 2951
Asp700I GAANNNNTTC 3 cut(s) 2192, 2788, 2952
AspA2I CCTAGG 1 cut(s) 553
AspLEI GCGC 2 cut(s) 424, 4039
AspS9I GGNCC 4 cut(s) 382, 688, 3275, 3497
AsuHPI GGTGA 9 cut(s) 236, 301, 317, 569, 3136, 3182, 3350, 3574, 4415
AsuII TTCGAA 3 cut(s) 3298, 3482, 3739
AvaI CYCGRG 1 cut(s) 614
AvaII GGWCC 3 cut(s) 382, 688, 3497
AvrII CCTAGG 1 cut(s) 553
BalI TGGCCA 3 cut(s) 84, 255, 2559
BamHI GGATCC 1 cut(s) 2541
BanI GGYRCC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 3486, 3518
BbsI GAAGAC 6 cut(s) 1349, 1421, 1709, 1781, 2367, 3872
BbvI GCAGC 1 cut(s) 376
BccI CCATC 6 cut(s) 216, 2471, 2826, 3329, 3957, 4070
BceAI ACGGC 1 cut(s) 120
BcgI CGANNNNNNTGC 4 cut(s) 198, 232, 2465, 2499
BciT130I CCWGG 3 cut(s) 158, 686, 3633
BclI TGATCA 2 cut(s) 2815, 3160
BcoDI GTCTC 5 cut(s) 147, 204, 858, 3649, 3982
BfmI CTRYAG 1 cut(s) 3396
BfoI RGCGCY 1 cut(s) 425
BfuAI ACCTGC 1 cut(s) 183
BglII AGATCT 4 cut(s) 457, 535, 3025, 3400
BisI GCNGC 1 cut(s) 365
BlnI CCTAGG 1 cut(s) 553
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 3 cut(s) 158, 686, 3633
Bme18I GGWCC 3 cut(s) 382, 688, 3497
BmeRI GACNNNNNGTC 1 cut(s) 3662
BmeT110I CYCGRG 1 cut(s) 614
BmgT120I GGNCC 4 cut(s) 382, 688, 3275, 3497
BmiI GGNNCC 4 cut(s) 113, 659, 2543, 3228
BmrFI CCNGG 3 cut(s) 158, 686, 3633
BmrI ACTGGG 1 cut(s) 4227
BmuI ACTGGG 1 cut(s) 4227
BoxI GACNNNNGTC 2 cut(s) 2581, 3076
BpiI GAAGAC 6 cut(s) 1349, 1421, 1709, 1781, 2367, 3872
BpmI CTGGAG 3 cut(s) 3601, 3654, 3673
Bpu14I TTCGAA 3 cut(s) 3298, 3482, 3739
BpuEI CTTGAG 1 cut(s) 790
BsaBI GATNNNNATC 1 cut(s) 2820
BsaI GGTCTC 1 cut(s) 147
BsaJI CCNNGG 3 cut(s) 79, 553, 1051
BsaWI WCCGGW 2 cut(s) 2842, 2864
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 2534, 3725, 4140
Bse1I ACTGG 6 cut(s) 256, 2560, 3487, 3618, 4222, 4294
Bse8I GATNNNNATC 1 cut(s) 2820
BseBI CCWGG 3 cut(s) 158, 686, 3633
BseDI CCNNGG 3 cut(s) 79, 553, 1051
BseJI GATNNNNATC 1 cut(s) 2820
BseLI CCNNNNNNNGG 4 cut(s) 121, 2534, 3725, 4140
BseMII CTCAG 5 cut(s) 400, 572, 683, 2891, 4077
BseNI ACTGG 6 cut(s) 256, 2560, 3487, 3618, 4222, 4294
BseRI GAGGAG 2 cut(s) 780, 2550
BseXI GCAGC 1 cut(s) 376
BseYI CCCAGC 2 cut(s) 2545, 2696
BshFI GGCC 6 cut(s) 84, 255, 552, 2559, 3277, 4091
BshNI GGYRCC 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 3 cut(s) 2843, 2865, 3278
BslI CCNNNNNNNGG 4 cut(s) 121, 2534, 3725, 4140
BsmAI GTCTC 5 cut(s) 147, 204, 858, 3649, 3982
BsmI GAATGC 1 cut(s) 2194
BsnI GGCC 6 cut(s) 84, 255, 552, 2559, 3277, 4091
Bso31I GGTCTC 1 cut(s) 147
BsoBI CYCGRG 1 cut(s) 614
Bsp119I TTCGAA 3 cut(s) 3298, 3482, 3739
Bsp1407I TGTACA 1 cut(s) 3806
BspACI CCGC 6 cut(s) 219, 804, 1023, 2904, 3048, 3119
BspANI GGCC 6 cut(s) 84, 255, 552, 2559, 3277, 4091
BspCNI CTCAG 5 cut(s) 399, 573, 684, 2892, 4078
BspLI GGNNCC 4 cut(s) 113, 659, 2543, 3228
BspMI ACCTGC 1 cut(s) 183
BspPI GGATC 6 cut(s) 857, 2536, 2549, 2889, 3543, 4006
BspT104I TTCGAA 3 cut(s) 3298, 3482, 3739
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 147
BsrBI CCGCTC 2 cut(s) 806, 2904
BsrGI TGTACA 1 cut(s) 3806
BsrI ACTGG 6 cut(s) 256, 2560, 3487, 3618, 4222, 4294
BssECI CCNNGG 3 cut(s) 79, 553, 1051
BssT1I CCWWGG 3 cut(s) 79, 553, 1051
Bst2UI CCWGG 3 cut(s) 158, 686, 3633
Bst4CI ACNGT 3 cut(s) 215, 3075, 3389
BstAPI GCANNNNNTGC 1 cut(s) 140
BstAUI TGTACA 1 cut(s) 3806
BstBI TTCGAA 3 cut(s) 3298, 3482, 3739
BstC8I GCNNGC 3 cut(s) 141, 409, 3958
BstEII GGTNACC 1 cut(s) 509
BstH2I RGCGCY 1 cut(s) 425
BstHHI GCGC 2 cut(s) 424, 4039
BstMAI GTCTC 5 cut(s) 147, 204, 858, 3649, 3982
BstMWI GCNNNNNNNGC 3 cut(s) 55, 140, 2565
BstNI CCWGG 3 cut(s) 158, 686, 3633
BstNSI RCATGY 5 cut(s) 29, 1644, 2582, 2649, 3331
BstPAI GACNNNNGTC 2 cut(s) 2581, 3076
BstPI GGTNACC 1 cut(s) 509
BstSCI CCNGG 3 cut(s) 156, 684, 3631
BstSFI CTRYAG 1 cut(s) 3396
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 6 cut(s) 1349, 1421, 1709, 1781, 2367, 3872
BstX2I RGATCY 8 cut(s) 457, 535, 2541, 2881, 3025, 3400, 3535, 3998
BstYI RGATCY 8 cut(s) 457, 535, 2541, 2881, 3025, 3400, 3535, 3998
BsuRI GGCC 6 cut(s) 84, 255, 552, 2559, 3277, 4091
BtsI GCAGTG 2 cut(s) 134, 235
BtsIMutI CAGTG 3 cut(s) 134, 235, 3861
BveI ACCTGC 1 cut(s) 183
Cac8I GCNNGC 3 cut(s) 141, 409, 3958
CaiI CAGNNNCTG 2 cut(s) 3446, 3860
CfoI GCGC 2 cut(s) 424, 4039
Cfr13I GGNCC 4 cut(s) 382, 688, 3275, 3497
CseI GACGC 1 cut(s) 3513
CsiI ACCWGGT 1 cut(s) 156
Csp6I GTAC 1 cut(s) 3807
CspCI CAANNNNNGTGG 2 cut(s) 4342, 4377
CviQI GTAC 1 cut(s) 3807
DraI TTTAAA 2 cut(s) 331, 678
DriI GACNNNNNGTC 1 cut(s) 3662
EaeI YGGCCR 3 cut(s) 82, 253, 2557
Eam1105I GACNNNNNGTC 1 cut(s) 3662
Eco130I CCWWGG 3 cut(s) 79, 553, 1051
Eco31I GGTCTC 1 cut(s) 147
Eco32I GATATC 1 cut(s) 530
Eco47I GGWCC 3 cut(s) 382, 688, 3497
Eco47III AGCGCT 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 614
Eco91I GGTNACC 1 cut(s) 509
EcoO65I GGTNACC 1 cut(s) 509
EcoRI GAATTC 2 cut(s) 2910, 3203
EcoRII CCWGG 3 cut(s) 156, 684, 3631
EcoRV GATATC 1 cut(s) 530
EcoT14I CCWWGG 3 cut(s) 79, 553, 1051
ErhI CCWWGG 3 cut(s) 79, 553, 1051
FalI AAGNNNNNCTT 2 cut(s) 3919, 3951
FauI CCCGC 1 cut(s) 3112
FbaI TGATCA 2 cut(s) 2815, 3160
FblI GTMKAC 2 cut(s) 2217, 4157
Fnu4HI GCNGC 1 cut(s) 365
Fsp4HI GCNGC 1 cut(s) 365
GlaI GCGC 2 cut(s) 423, 4038
GluI GCNGC 1 cut(s) 365
GsaI CCCAGC 2 cut(s) 2549, 2700
GsuI CTGGAG 3 cut(s) 3601, 3654, 3673
HaeII RGCGCY 1 cut(s) 425
HaeIII GGCC 6 cut(s) 84, 255, 552, 2559, 3277, 4091
HapII CCGG 3 cut(s) 2843, 2865, 3278
HgaI GACGC 1 cut(s) 3513
HhaI GCGC 2 cut(s) 424, 4039
Hin6I GCGC 2 cut(s) 422, 4037
HinP1I GCGC 2 cut(s) 422, 4037
HindIII AAGCTT 3 cut(s) 2719, 3811, 3842
HpaII CCGG 3 cut(s) 2843, 2865, 3278
HphI GGTGA 9 cut(s) 236, 301, 317, 569, 3136, 3182, 3350, 3574, 4415
Hpy166II GTNNAC 5 cut(s) 235, 2218, 2612, 3734, 4158
Hpy8I GTNNAC 5 cut(s) 235, 2218, 2612, 3734, 4158
HpyCH4III ACNGT 3 cut(s) 215, 3075, 3389
HpyCH4IV ACGT 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 140, 2565
HpySE526I ACGT 1 cut(s) 99
HspAI GCGC 2 cut(s) 422, 4037
Ksp22I TGATCA 2 cut(s) 2815, 3160
LmnI GCTCC 4 cut(s) 486, 663, 750, 3762
Lsp1109I GCAGC 1 cut(s) 376
MabI ACCWGGT 1 cut(s) 156
MaeII ACGT 1 cut(s) 99
MbiI CCGCTC 2 cut(s) 806, 2904
MfeI CAATTG 2 cut(s) 42, 3239
MflI RGATCY 8 cut(s) 457, 535, 2541, 2881, 3025, 3400, 3535, 3998
MlsI TGGCCA 3 cut(s) 84, 255, 2559
MluNI TGGCCA 3 cut(s) 84, 255, 2559
MlyI GAGTC 5 cut(s) 240, 1456, 1816, 2176, 2320
Mox20I TGGCCA 3 cut(s) 84, 255, 2559
MroXI GAANNNNTTC 3 cut(s) 2192, 2788, 2952
MscI TGGCCA 3 cut(s) 84, 255, 2559
MslI CAYNNNNRTG 5 cut(s) 30, 90, 1639, 3168, 3943
Msp20I TGGCCA 3 cut(s) 84, 255, 2559
MspA1I CMGCKG 1 cut(s) 580
MspI CCGG 3 cut(s) 2843, 2865, 3278
MspR9I CCNGG 3 cut(s) 158, 686, 3633
MunI CAATTG 2 cut(s) 42, 3239
Mva1269I GAATGC 1 cut(s) 2194
MvaI CCWGG 3 cut(s) 158, 686, 3633
MwoI GCNNNNNNNGC 3 cut(s) 55, 140, 2565
NlaIV GGNNCC 4 cut(s) 113, 659, 2543, 3228
NmuCI GTSAC 8 cut(s) 100, 2700, 3211, 3338, 3383, 3562, 4323, 4403
NspI RCATGY 5 cut(s) 29, 1644, 2582, 2649, 3331
NspV TTCGAA 3 cut(s) 3298, 3482, 3739
PciI ACATGT 2 cut(s) 2578, 3327
PctI GAATGC 1 cut(s) 2194
PdmI GAANNNNTTC 3 cut(s) 2192, 2788, 2952
PfoI TCCNGGA 1 cut(s) 3631
PkrI GCNGC 1 cut(s) 366
PleI GAGTC 5 cut(s) 239, 1456, 1816, 2176, 2320
PpsI GAGTC 5 cut(s) 239, 1456, 1816, 2176, 2320
PscI ACATGT 2 cut(s) 2578, 3327
PshAI GACNNNNGTC 2 cut(s) 2581, 3076
PshBI ATTAAT 1 cut(s) 2951
PsiI TTATAA 1 cut(s) 2523
Psp6I CCWGG 3 cut(s) 156, 684, 3631
PspEI GGTNACC 1 cut(s) 509
PspFI CCCAGC 2 cut(s) 2545, 2696
PspGI CCWGG 3 cut(s) 156, 684, 3631
PspN4I GGNNCC 4 cut(s) 113, 659, 2543, 3228
PspPI GGNCC 4 cut(s) 382, 688, 3275, 3497
PsrI GAACNNNNNNTAC 2 cut(s) 4141, 4173
PstNI CAGNNNCTG 2 cut(s) 3446, 3860
PsuI RGATCY 8 cut(s) 457, 535, 2541, 2881, 3025, 3400, 3535, 3998
PvuII CAGCTG 1 cut(s) 580
RsaI GTAC 1 cut(s) 3808
RsaNI GTAC 1 cut(s) 3807
RseI CAYNNNNRTG 5 cut(s) 30, 90, 1639, 3168, 3943
SatI GCNGC 1 cut(s) 365
Sau96I GGNCC 4 cut(s) 382, 688, 3275, 3497
SchI GAGTC 5 cut(s) 240, 1456, 1816, 2176, 2320
ScrFI CCNGG 3 cut(s) 158, 686, 3633
SexAI ACCWGGT 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 3396
SfuI TTCGAA 3 cut(s) 3298, 3482, 3739
SinI GGWCC 3 cut(s) 382, 688, 3497
SmiMI CAYNNNNRTG 5 cut(s) 30, 90, 1639, 3168, 3943
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
SsiI CCGC 6 cut(s) 219, 804, 1023, 2904, 3048, 3119
SspI AATATT 2 cut(s) 377, 885
StyD4I CCNGG 3 cut(s) 156, 684, 3631
StyI CCWWGG 3 cut(s) 79, 553, 1051
TaaI ACNGT 3 cut(s) 215, 3075, 3389
TaiI ACGT 1 cut(s) 102
TatI WGTACW 1 cut(s) 3806
TscAI CASTG 3 cut(s) 141, 242, 3861
TseFI GTSAC 8 cut(s) 100, 2700, 3211, 3338, 3383, 3562, 4323, 4403
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 8 cut(s) 100, 2700, 3211, 3338, 3383, 3562, 4323, 4403
TspRI CASTG 3 cut(s) 141, 242, 3861
VpaK11BI GGWCC 3 cut(s) 382, 688, 3497
VspI ATTAAT 1 cut(s) 2951
XbaI TCTAGA 4 cut(s) 496, 532, 3670, 4303
XceI RCATGY 5 cut(s) 29, 1644, 2582, 2649, 3331
XcmI CCANNNNNNNNNTGG 1 cut(s) 3366
XmaJI CCTAGG 1 cut(s) 553
XmiI GTMKAC 2 cut(s) 2217, 4157
XmnI GAANNNNTTC 3 cut(s) 2192, 2788, 2952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.