pycom07g15200

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001613
Physical Location & Seq
Reverse (-)
17338903 .. 17341208
2306 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g15200.1

Sequence Viewer

Length: 960 bp
ATGTCCAACAGTGACGATAATTGGGCTGCCAGAGATGCACGAGAGCTACTGGAGAATCTGTCATTCTCTAATGAGAATGTTGTACAGATGGCGAAGGCAAACTATTTTAAACATTTGCTGCAGCGTCTCACTACAGGACCGGAAGATGTAAAAATGATTATGGCATCAAATTTGGCGGAGATGGAATTGACCGACCACAATAAAGAATCTTTGGTTCAAGGAGGAGTAATGGGTCCGCTTCTTTACATGGTCGCACATGGTGACATTCCAATTAAAATGGTGGCTGTTAGAGCTCTTAGAAACCTCTCAAGCTTGCCTAAAAATGGTCTACAGATGATTCGAGAAGGTGCTGAACGCCCATTACTTGACCTTCTTTTCAATCTTAGTTCGTCATTGTCAAGTTTGCGTGAATACATAGCAGCCACAATTATGCAACTCTCCATTTCAATGGCGTCTCAAGAATCCAACCAGACACCCATTTCATTTTTGGAATCAGATGAAGATATTATCAAGCTTTTCTCTTTGATTAGCGTGATGGGGCCCAATGTACAGCAAAGCATCATTCGGACCTTCCACGCCTTATGTCAATCCCCCTCCACTACAAGTATCAAGACCAAGTTGATACAGTCCTCAGCTGTACAAGTATTGGTTCAGTTGTGTGAGCATGATGACCTAAACCTACGAGCAAGTGCTGTAAAGCTGTTCTCTTGCTTAGTAGAAGGCAGCAGTGAAGTCACCACCATCCTGGAGCATGTGAATCAGAAGTGCATTGAGACTATAATCAAGATCATCAAAACTTCTGATGATGAAGAGGAGGTCGCTTATTCAATGGGCATTATCTCTAACCTTCCCGAAAACACCGATCGATCACACAGTGGCTTATGGATGCTGGGGCACTACCTGTCATACTCAGTTTTCTCCAGAATAGCAAGGAGAATGGTCCCCATAGAAATCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_2 U-box

Protein Analysis

320

Amino Acids

35.51

Weight (kDa)

5.32

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 58 - 270 1e-09 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 328
AciI CCGC 2 cut(s) 176, 236
AcsI RAATTY 1 cut(s) 169
AcyI GRCGYC 1 cut(s) 452
AdeI CACNNNGTG 2 cut(s) 260, 875
AfaI GTAC 3 cut(s) 84, 549, 639
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 4 cut(s) 218, 379, 447, 828
AjnI CCWGG 1 cut(s) 744
AluBI AGCT 6 cut(s) 46, 293, 312, 514, 635, 700
AluI AGCT 6 cut(s) 46, 293, 312, 514, 635, 700
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 3 cut(s) 131, 459, 767
AoxI GGCC 1 cut(s) 539
ApaI GGGCCC 1 cut(s) 543
ApeKI GCWGC 5 cut(s) 26, 118, 121, 419, 723
ApoI RAATTY 1 cut(s) 169
AspS9I GGNCC 6 cut(s) 137, 233, 539, 540, 567, 940
AsuHPI GGTGA 2 cut(s) 272, 727
AvaII GGWCC 4 cut(s) 137, 233, 567, 940
BaeGI GKGCMC 2 cut(s) 543, 897
BanII GRGCYC 2 cut(s) 295, 543
BauI CACGAG 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 295
BbvCI CCTCAGC 1 cut(s) 631
BbvI GCAGC 5 cut(s) 13, 105, 133, 431, 735
BccI CCATC 4 cut(s) 82, 175, 529, 749
BciT130I CCWGG 1 cut(s) 746
BcoDI GTCTC 3 cut(s) 131, 459, 767
BfmI CTRYAG 3 cut(s) 119, 132, 329
BisI GCNGC 5 cut(s) 27, 119, 122, 420, 724
BlsI GCNGC 5 cut(s) 28, 120, 123, 421, 725
Bme1390I CCNGG 1 cut(s) 746
Bme18I GGWCC 4 cut(s) 137, 233, 567, 940
BmgT120I GGNCC 6 cut(s) 137, 233, 539, 540, 567, 940
BmiI GGNNCC 4 cut(s) 234, 540, 541, 942
BmrFI CCNGG 1 cut(s) 746
BmsI GCATC 4 cut(s) 25, 173, 567, 876
BpmI CTGGAG 3 cut(s) 71, 767, 904
Bpu10I CCTNAGC 1 cut(s) 631
BpuEI CTTGAG 2 cut(s) 292, 441
Bsa29I ATCGAT 1 cut(s) 865
BsaHI GRCGYC 1 cut(s) 452
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 1 cut(s) 746
BseCI ATCGAT 1 cut(s) 865
BseGI GGATG 2 cut(s) 741, 891
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMII CTCAG 2 cut(s) 645, 924
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 2 cut(s) 237, 827
BseSI GKGCMC 2 cut(s) 543, 897
BseXI GCAGC 5 cut(s) 13, 105, 133, 431, 735
BseYI CCCAGC 1 cut(s) 889
Bsh1285I CGRYCG 1 cut(s) 865
BshFI GGCC 1 cut(s) 541
BshVI ATCGAT 1 cut(s) 865
BsiEI CGRYCG 1 cut(s) 865
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 926
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 3 cut(s) 131, 459, 767
BsmBI CGTCTC 2 cut(s) 131, 459
BsmFI GGGAC 1 cut(s) 926
BsnI GGCC 1 cut(s) 541
Bsp120I GGGCCC 1 cut(s) 539
Bsp1286I GDGCHC 3 cut(s) 295, 543, 897
Bsp1407I TGTACA 3 cut(s) 82, 547, 637
Bsp143I GATC 3 cut(s) 786, 862, 866
BspACI CCGC 2 cut(s) 176, 236
BspANI GGCC 1 cut(s) 541
BspCNI CTCAG 2 cut(s) 644, 923
BspDI ATCGAT 1 cut(s) 865
BspLI GGNNCC 4 cut(s) 234, 540, 541, 942
BspMAI CTGCAG 1 cut(s) 123
BsrGI TGTACA 3 cut(s) 82, 547, 637
BsrI ACTGG 1 cut(s) 54
BssMI GATC 3 cut(s) 786, 862, 866
BssNI GRCGYC 1 cut(s) 452
BssSI CACGAG 1 cut(s) 39
Bst2BI CACGAG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 3 cut(s) 11, 627, 875
Bst6I CTCTTC 1 cut(s) 804
BstACI GRCGYC 1 cut(s) 452
BstAUI TGTACA 3 cut(s) 82, 547, 637
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 5 cut(s) 296, 383, 631, 712, 910
BstF5I GGATG 2 cut(s) 741, 891
BstKTI GATC 3 cut(s) 789, 865, 869
BstMAI GTCTC 3 cut(s) 131, 459, 767
BstMBI GATC 3 cut(s) 786, 862, 866
BstMCI CGRYCG 1 cut(s) 865
BstMWI GCNNNNNNNGC 2 cut(s) 35, 290
BstNI CCWGG 1 cut(s) 746
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 1 cut(s) 744
BstSFI CTRYAG 3 cut(s) 119, 132, 329
BstSLI GKGCMC 2 cut(s) 543, 897
BstV1I GCAGC 5 cut(s) 13, 105, 133, 431, 735
BstXI CCANNNNNNTGG 2 cut(s) 448, 745
Bsu15I ATCGAT 1 cut(s) 865
BsuRI GGCC 1 cut(s) 541
BsuTUI ATCGAT 1 cut(s) 865
BtsCI GGATG 2 cut(s) 741, 891
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 3 cut(s) 16, 733, 880
Cac8I GCNNGC 1 cut(s) 314
Cfr13I GGNCC 6 cut(s) 137, 233, 539, 540, 567, 940
ClaI ATCGAT 1 cut(s) 865
CseI GACGC 2 cut(s) 113, 441
Csp6I GTAC 3 cut(s) 83, 548, 638
CviAII CATG 4 cut(s) 247, 257, 665, 752
CviQI GTAC 3 cut(s) 83, 548, 638
DdeI CTNAG 5 cut(s) 296, 383, 631, 712, 910
DpnI GATC 3 cut(s) 788, 864, 868
DpnII GATC 3 cut(s) 786, 862, 866
DraI TTTAAA 1 cut(s) 109
DraIII CACNNNGTG 2 cut(s) 260, 875
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
EciI GGCGGA 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 293
Eco24I GRGCYC 2 cut(s) 295, 543
Eco47I GGWCC 4 cut(s) 137, 233, 567, 940
Eco53kI GAGCTC 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 744
EcoT38I GRGCYC 2 cut(s) 295, 543
Esp3I CGTCTC 2 cut(s) 131, 459
FaeI CATG 4 cut(s) 250, 260, 668, 755
FaqI GGGAC 1 cut(s) 926
FatI CATG 4 cut(s) 246, 256, 664, 751
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 5 cut(s) 27, 119, 122, 420, 724
FokI GGATG 2 cut(s) 728, 898
FriOI GRGCYC 2 cut(s) 295, 543
Fsp4HI GCNGC 5 cut(s) 27, 119, 122, 420, 724
GluI GCNGC 5 cut(s) 27, 119, 122, 420, 724
GsaI CCCAGC 1 cut(s) 893
GsuI CTGGAG 3 cut(s) 71, 767, 904
HaeIII GGCC 1 cut(s) 541
HapII CCGG 1 cut(s) 140
HgaI GACGC 2 cut(s) 113, 441
Hin1I GRCGYC 1 cut(s) 452
Hin1II CATG 4 cut(s) 250, 260, 668, 755
HindIII AAGCTT 2 cut(s) 310, 512
HinfI GANTC 6 cut(s) 55, 206, 337, 461, 491, 757
HpaII CCGG 1 cut(s) 140
HphI GGTGA 2 cut(s) 272, 727
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 4 cut(s) 496, 567, 762, 802
Hpy188III TCNNGA 6 cut(s) 341, 458, 610, 784, 851, 921
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 6 cut(s) 88, 338, 380, 580, 713, 857
HpyCH4III ACNGT 3 cut(s) 11, 627, 875
HpyCH4V TGCA 4 cut(s) 38, 121, 433, 768
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 290
HpyF3I CTNAG 5 cut(s) 296, 383, 631, 712, 910
Hsp92I GRCGYC 1 cut(s) 452
Hsp92II CATG 4 cut(s) 250, 260, 668, 755
Kzo9I GATC 3 cut(s) 786, 862, 866
LmnI GCTCC 1 cut(s) 748
Lsp1109I GCAGC 5 cut(s) 13, 105, 133, 431, 735
LweI GCATC 4 cut(s) 25, 173, 567, 876
MaeIII GTNAC 3 cut(s) 11, 260, 733
MalI GATC 3 cut(s) 788, 864, 868
MboI GATC 3 cut(s) 786, 862, 866
MboII GAAGA 3 cut(s) 155, 512, 821
MhlI GDGCHC 3 cut(s) 295, 543, 897
MluCI AATT 5 cut(s) 19, 169, 185, 270, 426
MmeI TCCRAC 2 cut(s) 30, 489
MnlI CCTC 6 cut(s) 215, 314, 604, 640, 805, 808
MseI TTAA 2 cut(s) 108, 273
MslI CAYNNNNRTG 2 cut(s) 428, 446
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 746
MvaI CCWGG 1 cut(s) 746
MwoI GCNNNNNNNGC 2 cut(s) 35, 290
NdeII GATC 3 cut(s) 786, 862, 866
NlaIII CATG 4 cut(s) 250, 260, 668, 755
NlaIV GGNNCC 4 cut(s) 234, 540, 541, 942
NmuCI GTSAC 3 cut(s) 11, 260, 733
NspI RCATGY 1 cut(s) 755
PfeI GAWTC 6 cut(s) 55, 206, 337, 461, 491, 757
PfoI TCCNGGA 1 cut(s) 744
PkrI GCNGC 5 cut(s) 28, 120, 123, 421, 725
Ple19I CGATCG 1 cut(s) 865
Psp124BI GAGCTC 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 744
PspFI CCCAGC 1 cut(s) 889
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 4 cut(s) 234, 540, 541, 942
PspOMI GGGCCC 1 cut(s) 539
PspPI GGNCC 6 cut(s) 137, 233, 539, 540, 567, 940
PsrI GAACNNNNNNTAC 2 cut(s) 345, 377
PstI CTGCAG 1 cut(s) 123
PvuI CGATCG 1 cut(s) 865
PvuII CAGCTG 1 cut(s) 635
RsaI GTAC 3 cut(s) 84, 549, 639
RsaNI GTAC 3 cut(s) 83, 548, 638
RseI CAYNNNNRTG 2 cut(s) 428, 446
SacI GAGCTC 1 cut(s) 295
SaqAI TTAA 2 cut(s) 108, 273
SatI GCNGC 5 cut(s) 27, 119, 122, 420, 724
Sau3AI GATC 3 cut(s) 786, 862, 866
Sau96I GGNCC 6 cut(s) 137, 233, 539, 540, 567, 940
ScrFI CCNGG 1 cut(s) 746
SduI GDGCHC 3 cut(s) 295, 543, 897
SfaNI GCATC 4 cut(s) 25, 173, 567, 876
SfcI CTRYAG 3 cut(s) 119, 132, 329
SinI GGWCC 4 cut(s) 137, 233, 567, 940
SmiMI CAYNNNNRTG 2 cut(s) 428, 446
SmlI CTYRAG 2 cut(s) 307, 456
SmoI CTYRAG 2 cut(s) 307, 456
Sse9I AATT 5 cut(s) 19, 169, 185, 270, 426
SsiI CCGC 2 cut(s) 176, 236
SstI GAGCTC 1 cut(s) 295
StyD4I CCNGG 1 cut(s) 744
TaaI ACNGT 3 cut(s) 11, 627, 875
TaqI TCGA 2 cut(s) 340, 865
TaqII GACCGA 1 cut(s) 206
TasI AATT 5 cut(s) 19, 169, 185, 270, 426
TatI WGTACW 3 cut(s) 82, 547, 637
TfiI GAWTC 6 cut(s) 55, 206, 337, 461, 491, 757
Tru1I TTAA 2 cut(s) 108, 273
Tru9I TTAA 2 cut(s) 108, 273
TscAI CASTG 3 cut(s) 16, 733, 880
TseFI GTSAC 3 cut(s) 11, 260, 733
TseI GCWGC 5 cut(s) 26, 118, 121, 419, 723
Tsp45I GTSAC 3 cut(s) 11, 260, 733
TspDTI ATGAA 3 cut(s) 471, 513, 822
TspRI CASTG 3 cut(s) 16, 733, 880
VpaK11BI GGWCC 4 cut(s) 137, 233, 567, 940
XapI RAATTY 1 cut(s) 169
XceI RCATGY 1 cut(s) 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.