MD07G1295700.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001613
Physical Location & Seq
Reverse (-)
35625775 .. 35630091
4317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1295700.v1.1.491

Sequence Viewer

Length: 3006 bp
ATGGCGAATGATAGTGTTATCAGTGCCTCATTCGTTCAGGTGTCTGAATTACTCTTTGAGACCGTACATGTAGGAAAGGAAGTCCTCGTTCAGAAAGAAAGTTTCAAAGTATTGTCAATGTACTTGGAGCCGACCTCTTCTATTCTGAAAGAGTTGTCAAAGCAGAATATTGAGTGTTCCGAAAGCTTGGCTAATGCTTTGAAGATTCTGAATCGAGAGGGTGAAGTCGCGAAGCAGCTAGCTCTAGATTGCAGCAAAAGAAACAAGGTGTATCTCTTAATCAATTGCCGGAATATTGTTAAGGCCCTAGAGAGAAGCACAAAAGAGATTGGTAGAGCGCTTGGTCTAATCCCATTGGCGTCGTTGGATGTTTCATCTGGTATAAGTAGTCAGATTAGCAAGCTGTGCAAGAATATGCTGGATGTTGAGTATCGAGCGGCTGTGGCTGAGGAAGAGATTGTGGACAAACTTGAGCTAGGAATACAAGAACAGAATGCTGATCCGTCCCGCGCAAAGAACTTGCTTGTTCGAATTGCTGAGGCTCTTGGGATCTCCGATGATCAGTCTGCATTGGAAAATGAACTCGAGGAGTTCAAACGTGAATTGGATGATACGAATAGAAAAGACCTGGAAGAAGATTTGCCAATGGAACAGATCATTGCTTTGCTTGAGAAGGCTAATGCTACTTCATCTGCTGGTGACAAAGAGAAGGAGTATTTTGAAAAGAGAAATTCTTTAGGTAAGCAACCATTGGAACCTCTTCAGAAATTTCAATGTCCATTGAGTCATGATATCATGGAGGATCCCGTGGAAACCTCCTCTCGTCACACGTTTGAGAGAAGCGAGATAGAGAAGTGGTTTGCAGAAGGTAACACTAACTGTCCTGTGACCAGTATTCCCGTGGACACTTCAGTTCTACAGCCCAACAAAGCACTAAAACGATCAATTGAAGAATGGAAGGAAAGGAATACCATCTTTGCAATCGTCTCTATTAAACCGAAACTCCAGTTAAATGAAGAGCAGGAGCAAGAAGTGCTTCAATCCTTAGATAACCTGCAGAACTTTTACATGGAAAGGGAGTTGCACCGTGAATGGGTGACAATTGAGGGCTACATTCCTGTCCTCATTAGACTTCTTGGTTCGAAAAGTCGAGAAATAAGAAAGCGTGCTCTTGCCATCGTATTGATCCTTGCAAAAGATGGTGAAGAAAATAAGGGGAGAATCATAAAAGTGGATAATGCACTTGAATCTATTGTCCGCTCGCTTGCACGTAGAAATGAAGAAAGCAAGTTAGCATTGCAGTTGTTACTGGTACTATCTGCAACTAGGGCTGCACGCGATTTAATGGGAAATGTTCAGGGCTGCATACTTCTCCTAGTGACAATGTTAAGCAGTGAAGATAGTCAAGTCATCCGAGATGTAAACGAGCTTCTAGAAAATCTGTCTTTCGTTGATCAGAACATTATACAGATGGCGAAAGCAAACTATTTTAAACCTTTGTTGAAGCTTCTTTCTTCAGGAGTGGAAGATGTTAAAATGCTTATGGCTAGAACTTTGTCGGAAATTGAACTGACAGACCACAACAAACTGTCCATTGTAAAAGACGGGGTACTAGGACAGCTTCTTGAACTGCTTTCGCAAGGTGATACTGAAAAGAGGAAAGTTGGCGTCAAGGCTCTTCTTCACCTCTCCAACTTACCACAAAATGGCCTGCAGATGGTTAGAGAAGGTGCAGCTGGACCGCTATTTGAACTTTTGTATTCTCACAGTTTATTATCGCCAACCTTGCGCGAGCAGGTAGCTGAGACAATTATGCACCTTGCCACATCAACCGCCACACAAGAAGCTGCACAGGAGCAGGTATCGTTGTTAGGCTCTGAAGAAGAAATCTTCAAGCTCTTTTCGCTAATATCTTTAACCGGACCTGACATACAAATAAACATTCTCAAAACCTTCCATGCAATGTGCCAATCTTCTTCTGGTTTTGACATCAGACTAAAGTTGAGACAGCTCTCTGCTGTCCAAGTATTGGTTCAATTATGCGAAGCTGATAGCCCTACCGTGAGGGCAAATGCCTTGAATCTGTTCCTTTCCTTGACAGAAGACGGCGAGGGCAGCACATTCTTGGAGCATGTAAGTCAAAGGTGCGTCGAGACCTTGCTAAGGATTATAAAAAGTTCTAATGATGTGGAGGAGATAGCTGCTGCAATGGGGATCATTGCTAATCTTCCAAAGGACGCTCAGATGGCGCAGTGGCTTGTAGATGCTGAGGCAGTTGAAATCATCTGCAATTGTCTAACTGATGGGAACAGAGATGCCTTGTATAGAAGGCGGGCAACAGAGAGTGCTGTTGGAGCTCTTTGTCATTTCACAGTTTCGACAAATCAGGAATGGCAGAAAAAAATAGCTGAAGCTGGTATTATACCAGTGCTGGTGCAGCTACTAACTTCTGGAACTTCTTTGACAAAACAATATGCCGCCATTTCACTTAAACAGCTATCAGAAAGCTCCAAAAGCTTGAGCAAGCCGATCAAGAAGCGCGCGGTTTTCCTGCGCTGCTTCTCTGCACTGGAACTTGGCTGCCCTGCACACCAAGGCATCTGTACGGTGGAATCTTCATTCTGCATAGTAAAGGCCAATGCTGTGGAACCCCTTGTGAAATTGCTTGGTGAGGAAGATTTCGGAGCTTGTGAAGCTTCTCTCGATGCACTTTTGACATTGCTTGATGACCAAGGAATAGAGCATGGAAGCAAAGTGCTCGATGAAGTGAAAGCCATCGCTCCGATTATAAAATTGCTGAGTTCACAAGTGCCCAGTTTGCAAGGAAAGTCTCTCAAGGCTTTGGAAATGATCTTTCGGGTGAATGAGTTGAATCTAAAGTATGGAACTTCAGCACATATGGCATTAGTGGATATCACTCAGATGAAAAATAGTGATATGAAATCTCTGGCTGCCAAGGTTCTTGCTCAATTGGGTGTGCTTGGCAAACAATCTTCTTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

1002

Amino Acids

110.88

Weight (kDa)

5.32

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 256 - 323 2.5e-15 U-box domain
zf-Nse PF11789 256 - 297 4e-06 Zinc-finger of the MIZ type in Nse subunit
ARM_PUB PF25598 445 - 605 3.4e-06 Plant U-box protein ARM repeat region
ARM_PUB PF25598 663 - 771 3.7e-06 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2171, 2789
Acc36I ACCTGC 3 cut(s) 1062, 1786, 1849
AccB7I CCANNNNNTGG 2 cut(s) 1706, 2029
AccBSI CCGCTC 2 cut(s) 437, 1260
AccII CGCG 6 cut(s) 230, 510, 1338, 1791, 2541, 2543
AciI CCGC 8 cut(s) 437, 508, 1258, 1742, 1833, 2332, 2478, 2543
AclWI GGATC 6 cut(s) 494, 557, 797, 810, 1180, 2222
AcsI RAATTY 2 cut(s) 730, 767
AcuI CTGAAG 6 cut(s) 746, 894, 1500, 1899, 2430, 2874
AcyI GRCGYC 2 cut(s) 359, 1668
AfaI GTAC 5 cut(s) 66, 122, 1314, 1611, 2605
AfeI AGCGCT 1 cut(s) 339
AfiI CCNNNNNNNGG 5 cut(s) 1093, 1706, 1717, 2029, 2163
AflIII ACRYGT 2 cut(s) 67, 828
AjnI CCWGG 1 cut(s) 627
AjuI GAANNNNNNNTTGG 2 cut(s) 2016, 2048
Alw21I GWGCWC 3 cut(s) 1171, 2359, 2760
Alw26I GTCTC 6 cut(s) 53, 991, 1799, 1999, 2147, 2835
AlwI GGATC 6 cut(s) 494, 557, 797, 810, 1180, 2222
Ama87I CYCGRG 1 cut(s) 584
Aor51HI AGCGCT 1 cut(s) 339
AoxI GGCC 3 cut(s) 303, 1708, 2634
ApoI RAATTY 2 cut(s) 730, 767
Asp700I GAANNNNTTC 3 cut(s) 759, 1035, 2084
AspLEI GCGC 7 cut(s) 340, 512, 1791, 2251, 2541, 2543, 2556
AspS9I GGNCC 3 cut(s) 304, 1739, 1922
AsuHPI GGTGA 8 cut(s) 233, 710, 1108, 1214, 1655, 1676, 2681, 2872
AsuII TTCGAA 2 cut(s) 529, 1142
AsuNHI GCTAGC 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 2 cut(s) 1739, 1922
BaeGI GKGCMC 1 cut(s) 2814
BamHI GGATCC 1 cut(s) 802
BanII GRGCYC 1 cut(s) 2359
BarI GAAGNNNNNNTAC 2 cut(s) 1413, 1445
BbsI GAAGAC 1 cut(s) 2109
Bbv12I GWGCWC 3 cut(s) 1171, 2359, 2760
BbvCI CCTCAGC 3 cut(s) 447, 537, 2268
BccI CCATC 8 cut(s) 980, 1184, 1193, 1465, 1711, 2239, 2297, 2783
BceAI ACGGC 1 cut(s) 2122
BcgI CGANNNNNNTGC 2 cut(s) 2740, 2774
BciT130I CCWGG 1 cut(s) 629
BclI TGATCA 2 cut(s) 559, 1453
BcoDI GTCTC 6 cut(s) 53, 991, 1799, 1999, 2147, 2835
BfaI CTAG 9 cut(s) 239, 245, 308, 476, 1326, 1376, 1433, 1548, 1613
BfmI CTRYAG 3 cut(s) 917, 1055, 1712
BfoI RGCGCY 1 cut(s) 341
BfuAI ACCTGC 3 cut(s) 1062, 1786, 1849
Bme1390I CCNGG 1 cut(s) 629
Bme18I GGWCC 2 cut(s) 1739, 1922
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 3 cut(s) 304, 1739, 1922
BmiI GGNNCC 4 cut(s) 129, 756, 804, 2649
BmrFI CCNGG 1 cut(s) 629
BmrI ACTGGG 1 cut(s) 2808
BmsI GCATC 4 cut(s) 2254, 2305, 2607, 2695
BmtI GCTAGC 1 cut(s) 242
BmuI ACTGGG 1 cut(s) 2808
BpiI GAAGAC 1 cut(s) 2109
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 1 cut(s) 989
Bpu10I CCTNAGC 4 cut(s) 447, 537, 2162, 2268
Bpu14I TTCGAA 2 cut(s) 529, 1142
BpuEI CTTGAG 4 cut(s) 491, 689, 2539, 2819
BsaAI YACGTR 1 cut(s) 1271
BsaHI GRCGYC 2 cut(s) 359, 1668
BsaI GGTCTC 2 cut(s) 53, 2147
BsaJI CCNNGG 5 cut(s) 807, 900, 2593, 2731, 2955
BsaWI WCCGGW 1 cut(s) 1919
BsaXI ACNNNNNCTCC 2 cut(s) 987, 1017
Bsc4I CCNNNNNNNGG 5 cut(s) 1093, 1706, 1717, 2029, 2163
Bse1I ACTGG 6 cut(s) 891, 1006, 1314, 2426, 2574, 2814
Bse3DI GCAATG 6 cut(s) 657, 1295, 1968, 2214, 2217, 2717
BseBI CCWGG 1 cut(s) 629
BseDI CCNNGG 5 cut(s) 807, 900, 2593, 2731, 2955
BseGI GGATG 4 cut(s) 373, 427, 613, 1410
BseLI CCNNNNNNNGG 5 cut(s) 1093, 1706, 1717, 2029, 2163
BseMI GCAATG 6 cut(s) 657, 1295, 1968, 2214, 2217, 2717
BseMII CTCAG 7 cut(s) 438, 528, 1794, 2255, 2259, 2789, 2933
BseNI ACTGG 6 cut(s) 891, 1006, 1314, 2426, 2574, 2814
BsePI GCGCGC 1 cut(s) 2539
BseRI GAGGAG 3 cut(s) 602, 808, 2207
BseSI GKGCMC 1 cut(s) 2814
BsgI GTGCAG 6 cut(s) 1317, 1752, 1833, 2456, 2550, 2571
Bsh1236I CGCG 6 cut(s) 230, 510, 1338, 1791, 2541, 2543
BshFI GGCC 3 cut(s) 305, 1710, 2636
BsiHKAI GWGCWC 3 cut(s) 1171, 2359, 2760
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 2 cut(s) 289, 1920
BslFI GGGAC 1 cut(s) 490
BslI CCNNNNNNNGG 5 cut(s) 1093, 1706, 1717, 2029, 2163
BsmAI GTCTC 6 cut(s) 53, 991, 1799, 1999, 2147, 2835
BsmBI CGTCTC 1 cut(s) 991
BsmFI GGGAC 1 cut(s) 490
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 3 cut(s) 305, 1710, 2636
Bso31I GGTCTC 2 cut(s) 53, 2147
BsoBI CYCGRG 1 cut(s) 584
Bsp119I TTCGAA 2 cut(s) 529, 1142
Bsp1286I GDGCHC 4 cut(s) 1171, 2359, 2760, 2814
Bsp68I TCGCGA 1 cut(s) 230
BspACI CCGC 8 cut(s) 437, 508, 1258, 1742, 1833, 2332, 2478, 2543
BspANI GGCC 3 cut(s) 305, 1710, 2636
BspCNI CTCAG 7 cut(s) 439, 529, 1795, 2254, 2260, 2790, 2932
BspFNI CGCG 6 cut(s) 230, 510, 1338, 1791, 2541, 2543
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 4 cut(s) 129, 756, 804, 2649
BspMAI CTGCAG 2 cut(s) 1059, 1716
BspMI ACCTGC 3 cut(s) 1062, 1786, 1849
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 6 cut(s) 494, 557, 797, 810, 1180, 2222
BspQI GCTCTTC 2 cut(s) 1011, 1683
BspT104I TTCGAA 2 cut(s) 529, 1142
BspTNI GGTCTC 2 cut(s) 53, 2147
BsrBI CCGCTC 2 cut(s) 437, 1260
BsrDI GCAATG 6 cut(s) 657, 1295, 1968, 2214, 2217, 2717
BsrI ACTGG 6 cut(s) 891, 1006, 1314, 2426, 2574, 2814
BssECI CCNNGG 5 cut(s) 807, 900, 2593, 2731, 2955
BssHII GCGCGC 1 cut(s) 2539
BssNI GRCGYC 2 cut(s) 359, 1668
BssT1I CCWWGG 3 cut(s) 2593, 2731, 2955
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 8 cut(s) 64, 881, 1088, 1590, 1769, 2062, 2374, 2608
Bst6I CTCTTC 5 cut(s) 142, 447, 765, 1011, 1683
BstACI GRCGYC 2 cut(s) 359, 1668
BstAPI GCANNNNNTGC 2 cut(s) 405, 1033
BstBAI YACGTR 1 cut(s) 1271
BstBI TTCGAA 2 cut(s) 529, 1142
BstDEI CTNAG 9 cut(s) 447, 537, 1045, 1803, 2162, 2241, 2268, 2798, 2919
BstDSI CCRYGG 2 cut(s) 807, 900
BstENI CCTNNNNNAGG 1 cut(s) 2161
BstF5I GGATG 4 cut(s) 373, 427, 613, 1410
BstFNI CGCG 6 cut(s) 230, 510, 1338, 1791, 2541, 2543
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 7 cut(s) 340, 512, 1791, 2251, 2541, 2543, 2556
BstMAI GTCTC 6 cut(s) 53, 991, 1799, 1999, 2147, 2835
BstNI CCWGG 1 cut(s) 629
BstNSI RCATGY 2 cut(s) 71, 2135
BstSCI CCNGG 1 cut(s) 627
BstSFI CTRYAG 3 cut(s) 917, 1055, 1712
BstSLI GKGCMC 1 cut(s) 2814
BstUI CGCG 6 cut(s) 230, 510, 1338, 1791, 2541, 2543
BstV2I GAAGAC 1 cut(s) 2109
BstX2I RGATCY 2 cut(s) 549, 802
BstXI CCANNNNNNTGG 1 cut(s) 2644
BstYI RGATCY 2 cut(s) 549, 802
BsuRI GGCC 3 cut(s) 305, 1710, 2636
BtgI CCRYGG 2 cut(s) 807, 900
BtgZI GCGATG 1 cut(s) 2761
BtsCI GGATG 4 cut(s) 373, 427, 613, 1410
BtsI GCAGTG 2 cut(s) 1399, 2258
BtsIMutI CAGTG 5 cut(s) 28, 1399, 2258, 2433, 2567
BtuMI TCGCGA 1 cut(s) 230
BveI ACCTGC 3 cut(s) 1062, 1786, 1849
CciI TCATGA 1 cut(s) 787
CfoI GCGC 7 cut(s) 340, 512, 1791, 2251, 2541, 2543, 2556
Cfr13I GGNCC 3 cut(s) 304, 1739, 1922
CseI GACGC 4 cut(s) 348, 1657, 2137, 2246
Csp6I GTAC 5 cut(s) 65, 121, 1313, 1610, 2604
CviAII CATG 7 cut(s) 68, 788, 796, 1069, 1958, 2132, 2744
CviQI GTAC 5 cut(s) 65, 121, 1313, 1610, 2604
DdeI CTNAG 9 cut(s) 447, 537, 1045, 1803, 2162, 2241, 2268, 2798, 2919
DraI TTTAAA 1 cut(s) 1492
Eam1104I CTCTTC 5 cut(s) 142, 447, 765, 1011, 1683
EarI CTCTTC 5 cut(s) 142, 447, 765, 1011, 1683
Ecl136II GAGCTC 1 cut(s) 2357
Eco130I CCWWGG 3 cut(s) 2593, 2731, 2955
Eco24I GRGCYC 1 cut(s) 2359
Eco31I GGTCTC 2 cut(s) 53, 2147
Eco32I GATATC 2 cut(s) 793, 2914
Eco47I GGWCC 2 cut(s) 1739, 1922
Eco47III AGCGCT 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 2357
Eco57I CTGAAG 6 cut(s) 746, 894, 1500, 1899, 2430, 2874
Eco88I CYCGRG 1 cut(s) 584
EcoICRI GAGCTC 1 cut(s) 2357
EcoNI CCTNNNNNAGG 1 cut(s) 2161
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 627
EcoRV GATATC 2 cut(s) 793, 2914
EcoT14I CCWWGG 3 cut(s) 2593, 2731, 2955
EcoT38I GRGCYC 1 cut(s) 2359
ErhI CCWWGG 3 cut(s) 2593, 2731, 2955
Esp3I CGTCTC 1 cut(s) 991
FaeI CATG 7 cut(s) 71, 791, 799, 1072, 1961, 2135, 2747
FalI AAGNNNNNCTT 2 cut(s) 1020, 1052
FaqI GGGAC 1 cut(s) 490
FatI CATG 7 cut(s) 67, 787, 795, 1068, 1957, 2131, 2743
FauI CCCGC 2 cut(s) 515, 2325
FauNDI CATATG 1 cut(s) 2898
FbaI TGATCA 2 cut(s) 559, 1453
FokI GGATG 4 cut(s) 380, 434, 620, 1397
FriOI GRGCYC 1 cut(s) 2359
FspBI CTAG 9 cut(s) 239, 245, 308, 476, 1326, 1376, 1433, 1548, 1613
GlaI GCGC 7 cut(s) 339, 511, 1790, 2250, 2540, 2542, 2555
GsuI CTGGAG 1 cut(s) 989
HaeII RGCGCY 1 cut(s) 341
HaeIII GGCC 3 cut(s) 305, 1710, 2636
HapII CCGG 2 cut(s) 289, 1920
HgaI GACGC 4 cut(s) 348, 1657, 2137, 2246
HhaI GCGC 7 cut(s) 340, 512, 1791, 2251, 2541, 2543, 2556
Hin1I GRCGYC 2 cut(s) 359, 1668
Hin1II CATG 7 cut(s) 71, 791, 799, 1072, 1961, 2135, 2747
Hin6I GCGC 7 cut(s) 338, 510, 1789, 2249, 2539, 2541, 2554
HinP1I GCGC 7 cut(s) 338, 510, 1789, 2249, 2539, 2541, 2554
HindIII AAGCTT 4 cut(s) 184, 1505, 2515, 2694
HinfI GANTC 8 cut(s) 205, 211, 784, 1221, 1247, 2080, 2612, 2872
HpaII CCGG 2 cut(s) 289, 1920
HphI GGTGA 8 cut(s) 233, 710, 1108, 1214, 1655, 1676, 2681, 2872
Hpy166II GTNNAC 4 cut(s) 463, 904, 1423, 2804
Hpy8I GTNNAC 4 cut(s) 463, 904, 1423, 2804
Hpy99I CGWCG 2 cut(s) 364, 2153
HpyAV CCTTC 7 cut(s) 667, 703, 860, 952, 1721, 1963, 2322
HpyCH4III ACNGT 8 cut(s) 64, 881, 1088, 1590, 1769, 2062, 2374, 2608
HpyCH4IV ACGT 3 cut(s) 598, 830, 1270
HpyF3I CTNAG 9 cut(s) 447, 537, 1045, 1803, 2162, 2241, 2268, 2798, 2919
HpySE526I ACGT 3 cut(s) 598, 830, 1270
Hsp92I GRCGYC 2 cut(s) 359, 1668
Hsp92II CATG 7 cut(s) 71, 791, 799, 1072, 1961, 2135, 2747
HspAI GCGC 7 cut(s) 338, 510, 1789, 2249, 2539, 2541, 2554
Ksp22I TGATCA 2 cut(s) 559, 1453
LguI GCTCTTC 2 cut(s) 1011, 1683
LmnI GCTCC 8 cut(s) 127, 1024, 1855, 2128, 2354, 2513, 2684, 2785
LweI GCATC 4 cut(s) 2254, 2305, 2607, 2695
MaeI CTAG 9 cut(s) 239, 245, 308, 476, 1326, 1376, 1433, 1548, 1613
MaeII ACGT 3 cut(s) 598, 830, 1270
MaeIII GTNAC 7 cut(s) 698, 824, 869, 886, 1096, 1305, 1378
MbiI CCGCTC 2 cut(s) 437, 1260
MfeI CAATTG 5 cut(s) 283, 945, 1101, 2290, 2969
MflI RGATCY 2 cut(s) 549, 802
MhlI GDGCHC 4 cut(s) 1171, 2359, 2760, 2814
MlyI GAGTC 1 cut(s) 793
MmeI TCCRAC 4 cut(s) 345, 1539, 1716, 2332
MroXI GAANNNNTTC 3 cut(s) 759, 1035, 2084
MslI CAYNNNNRTG 2 cut(s) 1229, 2921
MspA1I CMGCKG 1 cut(s) 1736
MspI CCGG 2 cut(s) 289, 1920
MspR9I CCNGG 1 cut(s) 629
MunI CAATTG 5 cut(s) 283, 945, 1101, 2290, 2969
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 629
MvnI CGCG 6 cut(s) 230, 510, 1338, 1791, 2541, 2543
NdeI CATATG 1 cut(s) 2898
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 7 cut(s) 71, 791, 799, 1072, 1961, 2135, 2747
NlaIV GGNNCC 4 cut(s) 129, 756, 804, 2649
NmuCI GTSAC 5 cut(s) 698, 824, 886, 1096, 1378
NruI TCGCGA 1 cut(s) 230
NspI RCATGY 2 cut(s) 71, 2135
NspV TTCGAA 2 cut(s) 529, 1142
PaeR7I CTCGAG 1 cut(s) 584
PagI TCATGA 1 cut(s) 787
PauI GCGCGC 1 cut(s) 2539
PciI ACATGT 1 cut(s) 67
PciSI GCTCTTC 2 cut(s) 1011, 1683
PctI GAATGC 1 cut(s) 499
PdmI GAANNNNTTC 3 cut(s) 759, 1035, 2084
PfeI GAWTC 7 cut(s) 205, 211, 1221, 1247, 2080, 2612, 2872
PflMI CCANNNNNTGG 2 cut(s) 1706, 2029
PleI GAGTC 1 cut(s) 792
PpsI GAGTC 1 cut(s) 792
Ppu21I YACGTR 1 cut(s) 1271
PscI ACATGT 1 cut(s) 67
PsiI TTATAA 2 cut(s) 2171, 2789
Psp124BI GAGCTC 1 cut(s) 2359
Psp6I CCWGG 1 cut(s) 627
PspGI CCWGG 1 cut(s) 627
PspN4I GGNNCC 4 cut(s) 129, 756, 804, 2649
PspPI GGNCC 3 cut(s) 304, 1739, 1922
PspXI VCTCGAGB 1 cut(s) 584
PstI CTGCAG 2 cut(s) 1059, 1716
PsuI RGATCY 2 cut(s) 549, 802
PteI GCGCGC 1 cut(s) 2539
PvuII CAGCTG 1 cut(s) 1736
RruI TCGCGA 1 cut(s) 230
RsaI GTAC 5 cut(s) 66, 122, 1314, 1611, 2605
RsaNI GTAC 5 cut(s) 65, 121, 1313, 1610, 2604
RseI CAYNNNNRTG 2 cut(s) 1229, 2921
SacI GAGCTC 1 cut(s) 2359
SapI GCTCTTC 2 cut(s) 1011, 1683
Sau96I GGNCC 3 cut(s) 304, 1739, 1922
SchI GAGTC 1 cut(s) 793
ScrFI CCNGG 1 cut(s) 629
SduI GDGCHC 4 cut(s) 1171, 2359, 2760, 2814
SfaNI GCATC 4 cut(s) 2254, 2305, 2607, 2695
SfcI CTRYAG 3 cut(s) 917, 1055, 1712
Sfr274I CTCGAG 1 cut(s) 584
SfuI TTCGAA 2 cut(s) 529, 1142
SinI GGWCC 2 cut(s) 1739, 1922
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 2 cut(s) 1229, 2921
SmlI CTYRAG 5 cut(s) 470, 584, 668, 2518, 2834
SmoI CTYRAG 5 cut(s) 470, 584, 668, 2518, 2834
SsiI CCGC 8 cut(s) 437, 508, 1258, 1742, 1833, 2332, 2478, 2543
SspI AATATT 2 cut(s) 169, 295
SspMI CTAG 9 cut(s) 239, 245, 308, 476, 1326, 1376, 1433, 1548, 1613
SstI GAGCTC 1 cut(s) 2359
StyD4I CCNGG 1 cut(s) 627
StyI CCWWGG 3 cut(s) 2593, 2731, 2955
TaaI ACNGT 8 cut(s) 64, 881, 1088, 1590, 1769, 2062, 2374, 2608
TaiI ACGT 3 cut(s) 601, 833, 1273
TatI WGTACW 1 cut(s) 120
TauI GCSGC 2 cut(s) 440, 2480
TfiI GAWTC 7 cut(s) 205, 211, 1221, 1247, 2080, 2612, 2872
TscAI CASTG 5 cut(s) 28, 1399, 2258, 2433, 2574
TseFI GTSAC 5 cut(s) 698, 824, 886, 1096, 1378
Tsp45I GTSAC 5 cut(s) 698, 824, 886, 1096, 1378
TspDTI ATGAA 9 cut(s) 363, 594, 678, 1029, 1293, 2607, 2778, 2939, 2954
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 5 cut(s) 28, 1399, 2258, 2433, 2574
Van91I CCANNNNNTGG 2 cut(s) 1706, 2029
VpaK11BI GGWCC 2 cut(s) 1739, 1922
XagI CCTNNNNNAGG 1 cut(s) 2161
XapI RAATTY 2 cut(s) 730, 767
XbaI TCTAGA 2 cut(s) 244, 1432
XceI RCATGY 2 cut(s) 71, 2135
XcmI CCANNNNNNNNNTGG 2 cut(s) 898, 1976
XhoI CTCGAG 1 cut(s) 584
XmnI GAANNNNTTC 3 cut(s) 759, 1035, 2084
XspI CTAG 9 cut(s) 239, 245, 308, 476, 1326, 1376, 1433, 1548, 1613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.