MD01G1092300.v1.1
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007535
Physical Location & Seq
Reverse (-)
20694118 .. 20698517
4400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1092300.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGAAAAACTTCAGCAGCAGCAGCAGCAGCACTTCCATGATCACCACAACCACCGCCCAATCGGTGCCGGACATGGTGGCTGCGATCAGATTCCATCCCACTGAAGATGAAAAGGCCGACTTCTTGAGGAAGAAGATGAAAAATCACAATTCTCAAGCCTGCCTCTTCCTCCCTGTCATCGATGTCCGCAAGTTCGAGCCTTGGGAATTGCCTGGGCGCATGTTCGCTGAATCTCCGTATCAAGCTAAGGCGTGGTACTCCTTCAGCTGGTGTGATTACAAATCCAACAACAGTCCTCGCTTCAAAAGGAAAACAAAGAAAGGCTTCTGGAAGATGAACGGCGGCCAACGGGATGTCGGCCCGAAATATTTCACTTGCAAAAAGAGGACATTAACCTTCAAAAAAAAGACCTTCCAGGAAGGTCGTAGGTCTAAATCCGTTACGACGAGCTGGAAAATGCACGAGTACATTCGCATTAAACCTGAACGGGGTTCTAGTCCCCATCAGGCGAGGGATCAGCAAAGGGGCTTTGTTCTCTGCGTCCTCAAGTATAAGCCAGTTGATGAAGCTGCTACTGCTGCTGCAACGAATCATATGTCTCCAGACACTGAAGAAGTTCTGGCGTACAAACAGCTAGAACATGATCTGCTTGGTAGTGGAAATCAAGATGATGGTGAACCTGCTGGTGGCAGCTCATCTGATATTAATCAATCTCTACGCGACATGATTCAAGAGGTTTGGTTATGTTCCCCAGCAGGTGAAGATCTGAATTCACTCTTTCCTCTTCCTGAGCCACCTCAGCCTCATCAGCCACCACAGCTTGGAAATGCTCCTGATGTCTGTAGTGGCGAATTAGTTGATCCTGGTACTAGTGGCTGCATTACCTATAATACTGATAACCAAGCTGCAACAATGAATCATATGATTTCCGACACAGAAGAAATACTGGCATACAAACAGCTAGAACGTGATGTGTTTGCCGGTGGTAATCACGATGTTGGTGAACGTGGTAGTGGCATTTTGACCTTTGGTGATGGTAATGATGAAACTGGTGGCTGGAAATTCTCTGATTTCGATGATCAAGCTGCATCTGATAGGTTTCAAGAGTTGTTTCTTGAGATGGGAGAAATTCTGAATTCATCTTCTCAACCGCTGCAGCCACATCAGCCACTGCAACCACATCAACCGCAGGAATACAGCCCCTCCACACTGCAGCAATCTTTATTCCCGGATAATATTTCTTATCCTGAATATAAGAATAATATTTCAACCAATGATCCCAATGAGCAAGTTAACAAACTGGCTAATGGTGTTCCAATTCAAGCTACAGATGAAAGAATTTCAGTGGCTTCTTTTAGCCCAGAAGAATATATGGGTCCTCTGTTTGATCCATCTTCGTTACAAGATTACTTGTTGCTGTCACCAATGAACACGGAACTGGGAGATGTTGTGCATACCAATAACTATAACTATATTGGATGCAATGATGAGTTGCGGATCTCTAGATTATATGGAGATTTCAACTCTTCTCCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

513

Amino Acids

57.48

Weight (kDa)

5.18

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 29 - 156 2.8e-21 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 746
Acc36I ACCTGC 2 cut(s) 688, 746
AccB1I GGYRCC 1 cut(s) 64
AccB7I CCANNNNNTGG 1 cut(s) 821
AccII CGCG 1 cut(s) 720
AciI CCGC 6 cut(s) 54, 187, 342, 1151, 1187, 1495
AclWI GGATC 5 cut(s) 522, 854, 1271, 1382, 1505
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 5 cut(s) 769, 1061, 1128, 1135, 1338
AcuI CTGAAG 3 cut(s) 123, 247, 630
AfaI GTAC 4 cut(s) 257, 467, 626, 868
AfiI CCNNNNNNNGG 4 cut(s) 267, 488, 686, 821
AgsI TTSAA 7 cut(s) 304, 400, 731, 1103, 1269, 1322, 1522
AhlI ACTAGT 1 cut(s) 869
AjnI CCWGG 3 cut(s) 211, 414, 862
Alw26I GTCTC 1 cut(s) 603
AlwI GGATC 5 cut(s) 522, 854, 1271, 1382, 1505
AlwNI CAGNNNCTG 2 cut(s) 608, 1171
AoxI GGCC 3 cut(s) 114, 343, 358
ApoI RAATTY 5 cut(s) 769, 1061, 1128, 1135, 1338
ArsI GACNNNNNNTTYG 2 cut(s) 581, 613
AseI ATTAAT 1 cut(s) 705
Asp700I GAANNNNTTC 4 cut(s) 8, 323, 368, 615
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 2 cut(s) 359, 1376
AsuC2I CCSGG 1 cut(s) 1229
AsuHPI GGTGA 6 cut(s) 34, 686, 770, 1013, 1043, 1413
AvaII GGWCC 1 cut(s) 1376
BanI GGYRCC 1 cut(s) 64
BauI CACGAG 1 cut(s) 461
BbvCI CCTCAGC 1 cut(s) 798
BccI CCATC 6 cut(s) 102, 510, 665, 1028, 1114, 1399
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 3 cut(s) 213, 416, 864
BclI TGATCA 2 cut(s) 39, 1078
BcnI CCSGG 1 cut(s) 1229
BcoDI GTCTC 1 cut(s) 603
BcuI ACTAGT 1 cut(s) 869
BfaI CTAG 5 cut(s) 495, 635, 870, 962, 1503
BfmI CTRYAG 4 cut(s) 841, 1154, 1211, 1326
BfuAI ACCTGC 2 cut(s) 688, 746
BglII AGATCT 1 cut(s) 763
Bme1390I CCNGG 4 cut(s) 213, 416, 864, 1229
Bme18I GGWCC 1 cut(s) 1376
BmgT120I GGNCC 2 cut(s) 359, 1376
BmiI GGNNCC 2 cut(s) 66, 1377
BmrFI CCNGG 4 cut(s) 213, 416, 864, 1229
BmrI ACTGGG 1 cut(s) 1448
BmsI GCATC 2 cut(s) 1097, 1469
BmuI ACTGGG 1 cut(s) 1448
BpmI CTGGAG 1 cut(s) 585
Bpu10I CCTNAGC 3 cut(s) 246, 789, 798
BpuEI CTTGAG 4 cut(s) 138, 145, 530, 1136
BpuMI CCSGG 1 cut(s) 1229
Bsa29I ATCGAT 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 705
BsaJI CCNNGG 2 cut(s) 200, 212
BsaXI ACNNNNNCTCC 4 cut(s) 1187, 1217, 1434, 1464
Bsc4I CCNNNNNNNGG 4 cut(s) 267, 488, 686, 821
Bse118I RCCGGY 1 cut(s) 980
Bse1I ACTGG 5 cut(s) 557, 951, 1054, 1305, 1443
Bse3DI GCAATG 1 cut(s) 1489
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 3 cut(s) 213, 416, 864
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 2 cut(s) 200, 212
BseGI GGATG 3 cut(s) 94, 358, 1484
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 4 cut(s) 267, 488, 686, 821
BseMI GCAATG 1 cut(s) 1489
BseMII CTCAG 2 cut(s) 780, 812
BseNI ACTGG 5 cut(s) 557, 951, 1054, 1305, 1443
BseYI CCCAGC 1 cut(s) 751
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 3 cut(s) 116, 345, 360
BshNI GGYRCC 1 cut(s) 64
BshVI ATCGAT 1 cut(s) 180
BsiSI CCGG 3 cut(s) 68, 981, 1229
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 4 cut(s) 267, 488, 686, 821
BsmAI GTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 3 cut(s) 116, 345, 360
BspACI CCGC 6 cut(s) 54, 187, 342, 1151, 1187, 1495
BspANI GGCC 3 cut(s) 116, 345, 360
BspCNI CTCAG 2 cut(s) 781, 811
BspDI ATCGAT 1 cut(s) 180
BspFNI CGCG 1 cut(s) 720
BspLI GGNNCC 2 cut(s) 66, 1377
BspMAI CTGCAG 2 cut(s) 1158, 1215
BspMI ACCTGC 2 cut(s) 688, 746
BspPI GGATC 5 cut(s) 522, 854, 1271, 1382, 1505
BspT107I GGYRCC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 1489
BsrFI RCCGGY 1 cut(s) 980
BsrI ACTGG 5 cut(s) 557, 951, 1054, 1305, 1443
BssAI RCCGGY 1 cut(s) 980
BssECI CCNNGG 2 cut(s) 200, 212
BssSI CACGAG 1 cut(s) 461
BssT1I CCWWGG 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 461
Bst2UI CCWGG 3 cut(s) 213, 416, 864
Bst4CI ACNGT 1 cut(s) 293
Bst6I CTCTTC 3 cut(s) 170, 789, 1531
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 3 cut(s) 246, 789, 798
BstF5I GGATG 3 cut(s) 94, 358, 1484
BstFNI CGCG 1 cut(s) 720
BstHHI GCGC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 603
BstMWI GCNNNNNNNGC 9 cut(s) 21, 24, 27, 575, 578, 799, 808, 817, 1165
BstNI CCWGG 3 cut(s) 213, 416, 864
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 4 cut(s) 211, 414, 862, 1227
BstSFI CTRYAG 4 cut(s) 841, 1154, 1211, 1326
BstUI CGCG 1 cut(s) 720
BstX2I RGATCY 2 cut(s) 763, 1497
BstYI RGATCY 2 cut(s) 763, 1497
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 3 cut(s) 116, 345, 360
BsuTUI ATCGAT 1 cut(s) 180
BtsCI GGATG 3 cut(s) 94, 358, 1484
BtsI GCAGTG 2 cut(s) 1169, 1208
BtsIMutI CAGTG 5 cut(s) 99, 606, 1169, 1208, 1350
BveI ACCTGC 2 cut(s) 688, 746
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 2 cut(s) 608, 1171
CfoI GCGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 980
Cfr13I GGNCC 2 cut(s) 359, 1376
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 1 cut(s) 529
Csp6I GTAC 4 cut(s) 256, 466, 625, 867
CviAII CATG 5 cut(s) 37, 73, 220, 641, 724
CviQI GTAC 4 cut(s) 256, 466, 625, 867
DdeI CTNAG 3 cut(s) 246, 789, 798
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 3 cut(s) 170, 789, 1531
EarI CTCTTC 3 cut(s) 170, 789, 1531
Eco130I CCWWGG 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 1376
Eco57I CTGAAG 3 cut(s) 123, 247, 630
EcoO109I RGGNCCY 1 cut(s) 1376
EcoRI GAATTC 2 cut(s) 769, 1135
EcoRII CCWGG 3 cut(s) 211, 414, 862
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 5 cut(s) 40, 76, 223, 644, 727
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 308, 340
FaqI GGGAC 1 cut(s) 483
FatI CATG 5 cut(s) 36, 72, 219, 640, 723
FauNDI CATATG 2 cut(s) 594, 921
FbaI TGATCA 2 cut(s) 39, 1078
FokI GGATG 3 cut(s) 81, 365, 1491
FspBI CTAG 5 cut(s) 495, 635, 870, 962, 1503
GlaI GCGC 1 cut(s) 218
GsaI CCCAGC 1 cut(s) 755
GsuI CTGGAG 1 cut(s) 585
HaeIII GGCC 3 cut(s) 116, 345, 360
HapII CCGG 3 cut(s) 68, 981, 1229
HgaI GACGC 1 cut(s) 529
HhaI GCGC 1 cut(s) 219
Hin1II CATG 5 cut(s) 40, 76, 223, 644, 727
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HinfI GANTC 5 cut(s) 90, 230, 589, 727, 916
HpaI GTTAAC 1 cut(s) 1294
HpaII CCGG 3 cut(s) 68, 981, 1229
HphI GGTGA 6 cut(s) 34, 686, 770, 1013, 1043, 1413
Hpy166II GTNNAC 3 cut(s) 677, 1004, 1294
Hpy188I TCNGA 7 cut(s) 89, 700, 768, 931, 1069, 1093, 1134
Hpy8I GTNNAC 3 cut(s) 677, 1004, 1294
Hpy99I CGWCG 1 cut(s) 448
HpyAV CCTTC 4 cut(s) 271, 406, 413, 421
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 2 cut(s) 967, 1006
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 24, 27, 575, 578, 799, 808, 817, 1165
HpyF3I CTNAG 3 cut(s) 246, 789, 798
HpySE526I ACGT 2 cut(s) 967, 1006
Hsp92II CATG 5 cut(s) 40, 76, 223, 644, 727
HspAI GCGC 1 cut(s) 217
Ksp22I TGATCA 2 cut(s) 39, 1078
KspAI GTTAAC 1 cut(s) 1294
LmnI GCTCC 1 cut(s) 835
LweI GCATC 2 cut(s) 1097, 1469
MaeI CTAG 5 cut(s) 495, 635, 870, 962, 1503
MaeII ACGT 2 cut(s) 967, 1006
MaeIII GTNAC 3 cut(s) 439, 1398, 1419
MflI RGATCY 2 cut(s) 763, 1497
MluCI AATT 9 cut(s) 148, 206, 769, 851, 1061, 1128, 1135, 1317, 1338
MmeI TCCRAC 2 cut(s) 309, 954
MroXI GAANNNNTTC 4 cut(s) 8, 323, 368, 615
MseI TTAA 4 cut(s) 392, 477, 705, 1293
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 2 cut(s) 267, 1153
MspI CCGG 3 cut(s) 68, 981, 1229
MspR9I CCNGG 4 cut(s) 213, 416, 864, 1229
MvaI CCWGG 3 cut(s) 213, 416, 864
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 9 cut(s) 21, 24, 27, 575, 578, 799, 808, 817, 1165
NciI CCSGG 1 cut(s) 1229
NdeI CATATG 2 cut(s) 594, 921
NlaIII CATG 5 cut(s) 40, 76, 223, 644, 727
NlaIV GGNNCC 2 cut(s) 66, 1377
NmuCI GTSAC 1 cut(s) 1419
NspI RCATGY 1 cut(s) 223
PaqCI CACCTGC 1 cut(s) 746
PdmI GAANNNNTTC 4 cut(s) 8, 323, 368, 615
PfeI GAWTC 5 cut(s) 90, 230, 589, 727, 916
PflMI CCANNNNNTGG 1 cut(s) 821
PfoI TCCNGGA 2 cut(s) 414, 1227
PpuMI RGGWCCY 1 cut(s) 1376
PshBI ATTAAT 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 1376
Psp6I CCWGG 3 cut(s) 211, 414, 862
PspFI CCCAGC 1 cut(s) 751
PspGI CCWGG 3 cut(s) 211, 414, 862
PspN4I GGNNCC 2 cut(s) 66, 1377
PspPI GGNCC 2 cut(s) 359, 1376
PspPPI RGGWCCY 1 cut(s) 1376
PstI CTGCAG 2 cut(s) 1158, 1215
PstNI CAGNNNCTG 2 cut(s) 608, 1171
PsuI RGATCY 2 cut(s) 763, 1497
PvuII CAGCTG 1 cut(s) 267
RsaI GTAC 4 cut(s) 257, 467, 626, 868
RsaNI GTAC 4 cut(s) 256, 466, 625, 867
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 4 cut(s) 392, 477, 705, 1293
Sau96I GGNCC 2 cut(s) 359, 1376
ScrFI CCNGG 4 cut(s) 213, 416, 864, 1229
SfaNI GCATC 2 cut(s) 1097, 1469
SfcI CTRYAG 4 cut(s) 841, 1154, 1211, 1326
SinI GGWCC 1 cut(s) 1376
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 4 cut(s) 124, 153, 545, 1115
SmoI CTYRAG 4 cut(s) 124, 153, 545, 1115
SpeI ACTAGT 1 cut(s) 869
Sse9I AATT 9 cut(s) 148, 206, 769, 851, 1061, 1128, 1135, 1317, 1338
SsiI CCGC 6 cut(s) 54, 187, 342, 1151, 1187, 1495
SspI AATATT 3 cut(s) 368, 1237, 1264
SspMI CTAG 5 cut(s) 495, 635, 870, 962, 1503
StyD4I CCNGG 4 cut(s) 211, 414, 862, 1227
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 2 cut(s) 970, 1009
TaqI TCGA 3 cut(s) 180, 195, 1074
TasI AATT 9 cut(s) 148, 206, 769, 851, 1061, 1128, 1135, 1317, 1338
TatI WGTACW 1 cut(s) 465
TauI GCSGC 1 cut(s) 345
TfiI GAWTC 5 cut(s) 90, 230, 589, 727, 916
Tru1I TTAA 4 cut(s) 392, 477, 705, 1293
Tru9I TTAA 4 cut(s) 392, 477, 705, 1293
TscAI CASTG 5 cut(s) 106, 613, 1176, 1215, 1350
TseFI GTSAC 1 cut(s) 1419
Tsp45I GTSAC 1 cut(s) 1419
TspGWI ACGGA 3 cut(s) 225, 427, 1448
TspRI CASTG 5 cut(s) 106, 613, 1176, 1215, 1350
Van91I CCANNNNNTGG 1 cut(s) 821
VpaK11BI GGWCC 1 cut(s) 1376
VspI ATTAAT 1 cut(s) 705
XapI RAATTY 5 cut(s) 769, 1061, 1128, 1135, 1338
XbaI TCTAGA 1 cut(s) 1502
XceI RCATGY 1 cut(s) 223
XmnI GAANNNNTTC 4 cut(s) 8, 323, 368, 615
XspI CTAG 5 cut(s) 495, 635, 870, 962, 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.