MD07G1163100.v1.1
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007535
Physical Location & Seq
Reverse (-)
23818801 .. 23822751
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1163100.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGGGAAACATCAGCAGCAGCACCGCCACATCAACAACCGGACAATCGGTGCCGGACATGGTGCTTGCGATCAGATTCCATCCCACTGAAGAGGAAATGGCAGACTTCTTGTGGAAGAAAATGAAAGGTCACAACTCCCAAGCCTGCCATTCCCTCCCTGTCATCGATGTCTGCAAGTTCGAGCCTTGGGATTTGCCTGAGCGCATGTTCCCTGACACTCGATATCAAGCTAAGGCATGGTACTCATTCAGCCAGCCTGATTACAAATACATCAATAGTCCTCGCTGCAACAGGATCACTGAGAAAGGCTTCTGGAAAATCACAGGCAAGCCACGAGATATCAGCTCCACACATGTCACTTGCAAAAAGAGAACCTTGACCTTCTACAAAGGTCGTGTGTCTAAATCCGAGAGGACTGGCTGGGTGATGCACGAGTACTATTTCACTAAACCTAAACAGGGTTCTAATACCAGTCAGGGGAGGGATCCGCTGAGGGACTTTGTTCTTTACCGCTTAAAGAAGAAGCCAGCTAATTCTATGTCTGATAACAAGAATCTTCAGGATACTTTGATCGGTGATGATTTAACTGATCCTGGTAATGGTAGTTGCATTGCATCTAGTTCCGGAAATGATCAAGCAGCTACTGCTGCTGCATTGAATCATGATATGACTCCAGCTGCAGAAGAACTTCTTGCATGCCAAGAGCTAGAAGATGATCTGTTTGACAGTGGAAATCATGATCGTGGCGAACCTGGTGGCGGCATCTCATCTGATTCTACTGATCAAGTTCTACGTGACATGATTCAACAGTGGAAGTCGTGTGCTCCGGTAGGTGAATATCTGAATTCACTCTTGACTCTTCCTGAGCCACCTCAGCCACCTCAGCCACCTCAGCCACCTCAGCTTGGAAATGCTCCTGATGTCTGTAGTGATGAATTCGTTGATCCTAGTACTGGTGGCTGCATCACCTGTAATACTGATAACCAAGATGCTGCAATGAATCATATGATTTCCGCTGAAGAAGAAATTCTGGCATACAAACAGCTAGAACGTGCTTTGCTTGGCAATGGCGATCATGATGATGGTGAACCTGGTAGCGGCGGTTCATCTGATTTTAATGATCAAGCTGTAGATGATATGATTCTGGAGCTTTCTGCGGAGCTACAAAAAGATTCGGATTCACCCTTTCCTCCTCCCGAGCCATCTCAGACAATTCAGCCACACCAGCTGGGAGATGCTGCGGACGTCCATAGTGGCAAATTCAGTAACTGTCCGTCTCCAACCAATTATGACAACGAATCAGTTATCAACATCGTTTATAATTCTCAAAATCGTGCTACAAGTGAAAGGATTTCCGAGGCTTATTCTCAGCGAGAAGAAAATCGGGAATCACCCTTTCAACCGTTTGATCATTACCCATTGCAGTCACCATTATTTTCCTCAGAACTGGGAGAGCTTATGCTTGCCAATACCTATATTGGAGGCAACGAGTCGCAATCTTTAAATATAGAGCCTGCGACTCTTTTCCCGCAAAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

514

Amino Acids

56.71

Weight (kDa)

4.65

Isoelectric Point (pI)

62.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 24 - 147 3.4e-29 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1320
AatII GACGTC 1 cut(s) 1248
AccB1I GGYRCC 1 cut(s) 49
AccIII TCCGGA 1 cut(s) 623
AclWI GGATC 5 cut(s) 302, 479, 492, 584, 938
AcsI RAATTY 4 cut(s) 844, 935, 1026, 1259
AcuI CTGAAG 3 cut(s) 108, 542, 1038
AcyI GRCGYC 1 cut(s) 1245
AfaI GTAC 3 cut(s) 242, 437, 952
AfiI CCNNNNNNNGG 7 cut(s) 458, 477, 599, 758, 905, 953, 1097
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 3 cut(s) 658, 806, 1400
AjnI CCWGG 3 cut(s) 592, 751, 1090
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 1 cut(s) 1281
AlwI GGATC 5 cut(s) 302, 479, 492, 584, 938
AlwNI CAGNNNCTG 2 cut(s) 644, 1269
Ama87I CYCGRG 1 cut(s) 1196
Aor13HI TCCGGA 1 cut(s) 623
ApoI RAATTY 4 cut(s) 844, 935, 1026, 1259
Asp700I GAANNNNTTC 3 cut(s) 308, 687, 1026
AspLEI GCGC 1 cut(s) 204
AsuHPI GGTGA 8 cut(s) 436, 587, 845, 958, 1097, 1173, 1383, 1419
AvaI CYCGRG 1 cut(s) 1196
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 1 cut(s) 49
BauI CACGAG 2 cut(s) 333, 431
Bbv12I GWGCWC 1 cut(s) 826
BbvCI CCTCAGC 5 cut(s) 491, 873, 882, 891, 900
BccI CCATC 3 cut(s) 87, 1076, 1210
BciT130I CCWGG 3 cut(s) 594, 753, 1092
BciVI GTATCC 1 cut(s) 556
BclI TGATCA 4 cut(s) 631, 781, 1120, 1408
BcoDI GTCTC 1 cut(s) 1281
BfaI CTAG 4 cut(s) 618, 707, 948, 1046
BfmI CTRYAG 3 cut(s) 678, 925, 1128
BfuI GTATCC 1 cut(s) 556
BmcAI AGTACT 2 cut(s) 437, 952
Bme1390I CCNGG 3 cut(s) 594, 753, 1092
BmeT110I CYCGRG 1 cut(s) 1196
BmiI GGNNCC 2 cut(s) 51, 486
BmrFI CCNGG 3 cut(s) 594, 753, 1092
BmrI ACTGGG 1 cut(s) 1457
BmsI GCATC 6 cut(s) 417, 623, 771, 972, 979, 1225
BmuI ACTGGG 1 cut(s) 1457
BpmI CTGGAG 2 cut(s) 657, 1166
Bpu10I CCTNAGC 8 cut(s) 198, 231, 491, 864, 873, 882, 891, 900
Bsa29I ATCGAT 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 794
BsaHI GRCGYC 1 cut(s) 1245
BsaJI CCNNGG 2 cut(s) 185, 1356
BsaWI WCCGGW 3 cut(s) 38, 623, 826
Bsc4I CCNNNNNNNGG 7 cut(s) 458, 477, 599, 758, 905, 953, 1097
Bse1I ACTGG 4 cut(s) 421, 471, 958, 1452
Bse3DI GCAATG 4 cut(s) 609, 1002, 1072, 1418
BseAI TCCGGA 1 cut(s) 623
BseBI CCWGG 3 cut(s) 594, 753, 1092
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 185, 1356
BseGI GGATG 1 cut(s) 79
BseLI CCNNNNNNNGG 7 cut(s) 458, 477, 599, 758, 905, 953, 1097
BseMI GCAATG 4 cut(s) 609, 1002, 1072, 1418
BseNI ACTGG 4 cut(s) 421, 471, 958, 1452
BseRI GAGGAG 1 cut(s) 1182
BseYI CCCAGC 2 cut(s) 420, 1228
BshNI GGYRCC 1 cut(s) 49
BshVI ATCGAT 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 826
BsiHKCI CYCGRG 1 cut(s) 1196
BsiSI CCGG 4 cut(s) 39, 53, 624, 827
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 7 cut(s) 458, 477, 599, 758, 905, 953, 1097
BsmAI GTCTC 1 cut(s) 1281
BsmBI CGTCTC 1 cut(s) 1281
BsmFI GGGAC 1 cut(s) 509
BsoBI CYCGRG 1 cut(s) 1196
Bsp1286I GDGCHC 1 cut(s) 826
Bsp13I TCCGGA 1 cut(s) 623
BspDI ATCGAT 1 cut(s) 165
BspEI TCCGGA 1 cut(s) 623
BspHI TCATGA 3 cut(s) 661, 736, 1075
BspLI GGNNCC 2 cut(s) 51, 486
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 5 cut(s) 302, 479, 492, 584, 938
BspT107I GGYRCC 1 cut(s) 49
BsrDI GCAATG 4 cut(s) 609, 1002, 1072, 1418
BsrI ACTGG 4 cut(s) 421, 471, 958, 1452
BssECI CCNNGG 2 cut(s) 185, 1356
BssNI GRCGYC 1 cut(s) 1245
BssSI CACGAG 2 cut(s) 333, 431
BssT1I CCWWGG 1 cut(s) 185
Bst2BI CACGAG 2 cut(s) 333, 431
Bst2UI CCWGG 3 cut(s) 594, 753, 1092
Bst4CI ACNGT 4 cut(s) 728, 810, 1271, 1404
Bst6I CTCTTC 2 cut(s) 84, 864
BstACI GRCGYC 1 cut(s) 1245
BstAPI GCANNNNNTGC 1 cut(s) 644
BstBAI YACGTR 1 cut(s) 794
BstC8I GCNNGC 8 cut(s) 66, 145, 254, 329, 528, 697, 1464, 1515
BstF5I GGATG 1 cut(s) 79
BstHHI GCGC 1 cut(s) 204
BstMAI GTCTC 1 cut(s) 1281
BstMWI GCNNNNNNNGC 7 cut(s) 644, 647, 874, 883, 892, 901, 1225
BstNI CCWGG 3 cut(s) 594, 753, 1092
BstNSI RCATGY 3 cut(s) 208, 356, 699
BstSCI CCNGG 3 cut(s) 592, 751, 1090
BstSFI CTRYAG 3 cut(s) 678, 925, 1128
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 165
BsuI GTATCC 1 cut(s) 556
BsuTUI ATCGAT 1 cut(s) 165
BtsCI GGATG 1 cut(s) 79
BtsIMutI CAGTG 4 cut(s) 84, 297, 733, 815
Cac8I GCNNGC 8 cut(s) 66, 145, 254, 329, 528, 697, 1464, 1515
CaiI CAGNNNCTG 2 cut(s) 644, 1269
CciI TCATGA 3 cut(s) 661, 736, 1075
CfoI GCGC 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 165
CsiI ACCWGGT 2 cut(s) 751, 1090
Csp6I GTAC 3 cut(s) 241, 436, 951
CviAII CATG 9 cut(s) 58, 205, 237, 353, 662, 696, 737, 799, 1076
CviQI GTAC 3 cut(s) 241, 436, 951
DraI TTTAAA 1 cut(s) 1503
Eam1104I CTCTTC 2 cut(s) 84, 864
EarI CTCTTC 2 cut(s) 84, 864
Eco130I CCWWGG 1 cut(s) 185
Eco32I GATATC 2 cut(s) 224, 340
Eco57I CTGAAG 3 cut(s) 108, 542, 1038
Eco88I CYCGRG 1 cut(s) 1196
EcoRI GAATTC 2 cut(s) 844, 935
EcoRII CCWGG 3 cut(s) 592, 751, 1090
EcoRV GATATC 2 cut(s) 224, 340
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
Esp3I CGTCTC 1 cut(s) 1281
FaeI CATG 9 cut(s) 61, 208, 240, 356, 665, 699, 740, 802, 1079
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 675, 707
FaqI GGGAC 1 cut(s) 509
FatI CATG 9 cut(s) 57, 204, 236, 352, 661, 695, 736, 798, 1075
FauI CCCGC 1 cut(s) 1536
FauNDI CATATG 1 cut(s) 1005
FbaI TGATCA 4 cut(s) 631, 781, 1120, 1408
FokI GGATG 1 cut(s) 66
FspBI CTAG 4 cut(s) 618, 707, 948, 1046
GlaI GCGC 1 cut(s) 203
GsaI CCCAGC 2 cut(s) 424, 1232
GsuI CTGGAG 2 cut(s) 657, 1166
HapII CCGG 4 cut(s) 39, 53, 624, 827
HhaI GCGC 1 cut(s) 204
Hin1I GRCGYC 1 cut(s) 1245
Hin1II CATG 9 cut(s) 61, 208, 240, 356, 665, 699, 740, 802, 1079
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HpaII CCGG 4 cut(s) 39, 53, 624, 827
HphI GGTGA 8 cut(s) 436, 587, 845, 958, 1097, 1173, 1383, 1419
Hpy166II GTNNAC 1 cut(s) 1088
Hpy8I GTNNAC 1 cut(s) 1088
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 4 cut(s) 728, 810, 1271, 1404
HpyCH4IV ACGT 3 cut(s) 793, 1051, 1245
HpyF10VI GCNNNNNNNGC 7 cut(s) 644, 647, 874, 883, 892, 901, 1225
HpySE526I ACGT 3 cut(s) 793, 1051, 1245
Hsp92I GRCGYC 1 cut(s) 1245
Hsp92II CATG 9 cut(s) 61, 208, 240, 356, 665, 699, 740, 802, 1079
HspAI GCGC 1 cut(s) 202
Kpn2I TCCGGA 1 cut(s) 623
Ksp22I TGATCA 4 cut(s) 631, 781, 1120, 1408
LmnI GCTCC 5 cut(s) 350, 829, 919, 1147, 1159
LweI GCATC 6 cut(s) 417, 623, 771, 972, 979, 1225
MabI ACCWGGT 2 cut(s) 751, 1090
MaeI CTAG 4 cut(s) 618, 707, 948, 1046
MaeII ACGT 3 cut(s) 793, 1051, 1245
MaeIII GTNAC 5 cut(s) 128, 355, 794, 1265, 1425
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 1 cut(s) 826
MluCI AATT 8 cut(s) 532, 844, 935, 1026, 1212, 1259, 1285, 1321
MlyI GAGTC 4 cut(s) 664, 850, 1499, 1513
MmeI TCCRAC 1 cut(s) 1304
MroI TCCGGA 1 cut(s) 623
MroXI GAANNNNTTC 3 cut(s) 308, 687, 1026
MseI TTAA 4 cut(s) 515, 584, 1116, 1502
MslI CAYNNNNRTG 2 cut(s) 741, 1080
MspA1I CMGCKG 4 cut(s) 490, 677, 1016, 1228
MspI CCGG 4 cut(s) 39, 53, 624, 827
MspR9I CCNGG 3 cut(s) 594, 753, 1092
MvaI CCWGG 3 cut(s) 594, 753, 1092
MwoI GCNNNNNNNGC 7 cut(s) 644, 647, 874, 883, 892, 901, 1225
NdeI CATATG 1 cut(s) 1005
NlaIII CATG 9 cut(s) 61, 208, 240, 356, 665, 699, 740, 802, 1079
NlaIV GGNNCC 2 cut(s) 51, 486
NmuCI GTSAC 4 cut(s) 128, 355, 794, 1425
NspI RCATGY 3 cut(s) 208, 356, 699
PaeI GCATGC 1 cut(s) 699
PagI TCATGA 3 cut(s) 661, 736, 1075
PciI ACATGT 1 cut(s) 352
PdmI GAANNNNTTC 3 cut(s) 308, 687, 1026
PleI GAGTC 4 cut(s) 664, 850, 1498, 1513
PpsI GAGTC 4 cut(s) 664, 850, 1498, 1513
Ppu21I YACGTR 1 cut(s) 794
PscI ACATGT 1 cut(s) 352
PsiI TTATAA 1 cut(s) 1320
Psp6I CCWGG 3 cut(s) 592, 751, 1090
PspFI CCCAGC 2 cut(s) 420, 1228
PspGI CCWGG 3 cut(s) 592, 751, 1090
PspN4I GGNNCC 2 cut(s) 51, 486
PsrI GAACNNNNNNTAC 2 cut(s) 1087, 1119
PstI CTGCAG 1 cut(s) 682
PstNI CAGNNNCTG 2 cut(s) 644, 1269
PsuI RGATCY 1 cut(s) 484
PvuII CAGCTG 2 cut(s) 677, 1228
RsaI GTAC 3 cut(s) 242, 437, 952
RsaNI GTAC 3 cut(s) 241, 436, 951
RseI CAYNNNNRTG 2 cut(s) 741, 1080
SaqAI TTAA 4 cut(s) 515, 584, 1116, 1502
ScaI AGTACT 2 cut(s) 437, 952
SchI GAGTC 4 cut(s) 664, 850, 1499, 1513
ScrFI CCNGG 3 cut(s) 594, 753, 1092
SduI GDGCHC 1 cut(s) 826
SexAI ACCWGGT 2 cut(s) 751, 1090
SfaNI GCATC 6 cut(s) 417, 623, 771, 972, 979, 1225
SfcI CTRYAG 3 cut(s) 678, 925, 1128
SmiMI CAYNNNNRTG 2 cut(s) 741, 1080
SphI GCATGC 1 cut(s) 699
Sse9I AATT 8 cut(s) 532, 844, 935, 1026, 1212, 1259, 1285, 1321
SspMI CTAG 4 cut(s) 618, 707, 948, 1046
StyD4I CCNGG 3 cut(s) 592, 751, 1090
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 4 cut(s) 728, 810, 1271, 1404
TaiI ACGT 3 cut(s) 796, 1054, 1248
TaqI TCGA 3 cut(s) 165, 180, 220
TasI AATT 8 cut(s) 532, 844, 935, 1026, 1212, 1259, 1285, 1321
TatI WGTACW 2 cut(s) 435, 950
TauI GCSGC 2 cut(s) 762, 1101
Tru1I TTAA 4 cut(s) 515, 584, 1116, 1502
Tru9I TTAA 4 cut(s) 515, 584, 1116, 1502
TscAI CASTG 4 cut(s) 91, 304, 733, 815
TseFI GTSAC 4 cut(s) 128, 355, 794, 1425
Tsp45I GTSAC 4 cut(s) 128, 355, 794, 1425
TspDTI ATGAA 4 cut(s) 137, 948, 1013, 1095
TspGWI ACGGA 1 cut(s) 1263
TspRI CASTG 4 cut(s) 91, 304, 733, 815
XapI RAATTY 4 cut(s) 844, 935, 1026, 1259
XceI RCATGY 3 cut(s) 208, 356, 699
XmnI GAANNNNTTC 3 cut(s) 308, 687, 1026
XspI CTAG 4 cut(s) 618, 707, 948, 1046
ZraI GACGTC 1 cut(s) 1246
ZrmI AGTACT 2 cut(s) 437, 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.