MD07G1162900.v1.1
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007535
Physical Location & Seq
Reverse (-)
23782702 .. 23785223
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1162900.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGTATAAAACATTTGAAGGAGAAGGAGGAGGGATGGGAAACAGCACCACCACCACCAAAACAACAACCGCACAATCGGTGCCGGACATGATGGCTGCGATCAGATTCCATCCCACTGAAAAGGAAATGGCAGACTTCTTGGGGAAGAAGATGAGAAGTCACAAATCCCAAGCCTGCCACTTCCTCCCTGTCATTGATGTCTATAAGTCCGAGCCTCGGGAATTGCCTGAACTCATGTTCTCTGATTCTCCCTTTCAAGCTAAGGCGTGGTTCTCCTTCAGCCTGTGTGATTACAAATACACAAATAGTCTTCGCCGCAACAGAGTCACAGAGCATGGCTTCTGGAAGATCACAGGCAAGCCGCGGGATATCAGCTCGAAATACTTCACTTGCAAAAGGAGGATATTAACCTTCCAAAGCAAGACCTTCGAAGAAGGTGGGGCGTCTAAATCCGTTAGGACTGGCTGGATCATACACGAGTACTATTGCTCTAAACCTAAGCAGGGTTCTAGTCCCTGTCAGGAGAGGGATGTGAAGAGGGACTATGTTCTCTGCCTCCTGAAGTATAAGCCAGTTGATTCTAAGTCTGATAAAAAGAAGCCTGAGGGTACATCGGTCTGTGATGATTTAGCTGATCCCGGTAATTGTGGCTACATTGCCTCTAGTCCCGGAGATGATCACACCACTTCTGCTGCTGCAATGATTCATATGACTCCAGATTCAGAAGAAGGTTTGGCATATAAAGAGCTAGAAGATGATCTACTTGGCAATGGAAATCATGACCATGGTGAACCTCTTGGCAGTGTCTCATCTGATTTAATTGATCAAGCTCTAAGTGCCATGATTCATGAGTGGCAGTCATATGCTCTGCTAGGAAGATATCTGGATTCACCCTTTCCTCCTCCCGAGGCACCTCAGCTGCATCAACCATCTCACCTTGGAAATGCTCCTGGTATCTATAGTCATGAATTACTTGATCCTGGTACTGGTGGCTGCATCACCTATAATACTAATAACCAAGCTGCTGCCATGAATCATATGATTCCAGTCACTGAAGAAATTCTGGCACACAAACAGCCAGATAGTTCTGTGCTTGGCAGTGGCGATGATGATGGTGGTGAACTTGGTAGCGGCATTTCATCTGATTTTAATGATCCAGCTGTAGATGATATGATTCTTTACGTAATGTTGTTCTCAGCTAGGAAAAGAATTGGATTCACCCTTATCTTCTCCCGAGCCACCTCAGCCACCTCAGCCACATCAGCCACACCAGCTGGGAAATGTTCTGGACATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

433

Amino Acids

47.4

Weight (kDa)

5.81

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 33 - 162 1.5e-23 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 79, 910
AccII CGCG 1 cut(s) 364
AciI CCGC 5 cut(s) 69, 316, 362, 364, 1131
AclWI GGATC 4 cut(s) 476, 629, 971, 1148
AcsI RAATTY 1 cut(s) 1059
AcuI CTGAAG 3 cut(s) 262, 581, 1074
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 3 cut(s) 482, 610, 985
AfiI CCNNNNNNNGG 3 cut(s) 216, 503, 986
AgsI TTSAA 2 cut(s) 17, 257
AjnI CCWGG 2 cut(s) 949, 979
Alw26I GTCTC 1 cut(s) 811
AlwI GGATC 4 cut(s) 476, 629, 971, 1148
AlwNI CAGNNNCTG 1 cut(s) 1052
Ama87I CYCGRG 3 cut(s) 216, 905, 1233
ApeKI GCWGC 7 cut(s) 95, 692, 695, 919, 993, 1022, 1025
ApoI RAATTY 1 cut(s) 1059
Asp700I GAANNNNTTC 2 cut(s) 383, 1059
AsuC2I CCSGG 2 cut(s) 639, 669
AsuHPI GGTGA 6 cut(s) 800, 882, 926, 991, 1130, 1210
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 3 cut(s) 216, 905, 1233
AxyI CCTNAGG 1 cut(s) 603
BaeI ACNNNNGTAYC 2 cut(s) 600, 633
BanI GGYRCC 2 cut(s) 79, 910
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
BauI CACGAG 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 302
BbvCI CCTCAGC 3 cut(s) 915, 1243, 1252
BbvI GCAGC 7 cut(s) 82, 679, 682, 906, 980, 1009, 1012
BccI CCATC 5 cut(s) 28, 85, 117, 937, 1106
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 2 cut(s) 951, 981
BclI TGATCA 2 cut(s) 676, 823
BcnI CCSGG 2 cut(s) 639, 669
BcoDI GTCTC 1 cut(s) 811
BfaI CTAG 5 cut(s) 510, 663, 749, 872, 1200
BfmI CTRYAG 2 cut(s) 958, 1161
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 4 cut(s) 639, 669, 951, 981
BmeT110I CYCGRG 3 cut(s) 216, 905, 1233
BmiI GGNNCC 2 cut(s) 81, 912
BmrFI CCNGG 4 cut(s) 639, 669, 951, 981
BmsI GCATC 2 cut(s) 931, 1005
BpiI GAAGAC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 5 cut(s) 261, 498, 915, 1243, 1252
Bpu14I TTCGAA 1 cut(s) 429
BpuMI CCSGG 2 cut(s) 639, 669
BsaAI YACGTR 1 cut(s) 1183
BsaHI GRCGYC 1 cut(s) 443
BsaJI CCNNGG 5 cut(s) 215, 362, 784, 906, 937
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 503, 986
Bse1I ACTGG 4 cut(s) 466, 572, 991, 1046
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 3 cut(s) 654, 705, 775
BseBI CCWGG 2 cut(s) 951, 981
BseDI CCNNGG 5 cut(s) 215, 362, 784, 906, 937
BseGI GGATG 4 cut(s) 39, 109, 535, 1291
BseLI CCNNNNNNNGG 3 cut(s) 216, 503, 986
BseMI GCAATG 3 cut(s) 654, 705, 775
BseMII CTCAG 5 cut(s) 594, 929, 1209, 1257, 1266
BseNI ACTGG 4 cut(s) 466, 572, 991, 1046
BseRI GAGGAG 2 cut(s) 42, 891
BseXI GCAGC 7 cut(s) 82, 679, 682, 906, 980, 1009, 1012
BseYI CCCAGC 1 cut(s) 1274
Bsh1236I CGCG 1 cut(s) 364
BshNI GGYRCC 2 cut(s) 79, 910
BsiHKCI CYCGRG 3 cut(s) 216, 905, 1233
BsiSI CCGG 3 cut(s) 83, 639, 669
BslFI GGGAC 3 cut(s) 498, 554, 651
BslI CCNNNNNNNGG 3 cut(s) 216, 503, 986
BsmAI GTCTC 1 cut(s) 811
BsmFI GGGAC 3 cut(s) 498, 554, 651
BsoBI CYCGRG 3 cut(s) 216, 905, 1233
Bsp119I TTCGAA 1 cut(s) 429
Bsp143I GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
Bsp19I CCATGG 1 cut(s) 784
BspACI CCGC 5 cut(s) 69, 316, 362, 364, 1131
BspCNI CTCAG 5 cut(s) 595, 928, 1208, 1256, 1265
BspFNI CGCG 1 cut(s) 364
BspHI TCATGA 3 cut(s) 778, 847, 964
BspLI GGNNCC 2 cut(s) 81, 912
BspPI GGATC 4 cut(s) 476, 629, 971, 1148
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 2 cut(s) 79, 910
BsrDI GCAATG 3 cut(s) 654, 705, 775
BsrI ACTGG 4 cut(s) 466, 572, 991, 1046
BssECI CCNNGG 5 cut(s) 215, 362, 784, 906, 937
BssMI GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
BssNI GRCGYC 1 cut(s) 443
BssSI CACGAG 1 cut(s) 476
BssT1I CCWWGG 2 cut(s) 784, 937
Bst2BI CACGAG 1 cut(s) 476
Bst2UI CCWGG 2 cut(s) 951, 981
Bst6I CTCTTC 1 cut(s) 530
BstACI GRCGYC 1 cut(s) 443
BstBAI YACGTR 1 cut(s) 1183
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 2 cut(s) 175, 359
BstDEI CTNAG 9 cut(s) 261, 498, 582, 603, 833, 915, 1195, 1243, 1252
BstDSI CCRYGG 2 cut(s) 362, 784
BstF5I GGATG 4 cut(s) 39, 109, 535, 1291
BstFNI CGCG 1 cut(s) 364
BstKTI GATC 9 cut(s) 102, 351, 471, 637, 679, 760, 826, 979, 1156
BstMAI GTCTC 1 cut(s) 811
BstMBI GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
BstMWI GCNNNNNNNGC 5 cut(s) 836, 1244, 1253, 1262, 1271
BstNI CCWGG 2 cut(s) 951, 981
BstSCI CCNGG 4 cut(s) 637, 667, 949, 979
BstSFI CTRYAG 2 cut(s) 958, 1161
BstSNI TACGTA 1 cut(s) 1183
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 7 cut(s) 82, 679, 682, 906, 980, 1009, 1012
BstV2I GAAGAC 1 cut(s) 302
Bsu36I CCTNAGG 1 cut(s) 603
BtgI CCRYGG 2 cut(s) 362, 784
BtgZI GCGATG 1 cut(s) 1119
BtsCI GGATG 4 cut(s) 39, 109, 535, 1291
BtsI GCAGTG 2 cut(s) 808, 1105
BtsIMutI CAGTG 4 cut(s) 114, 808, 1050, 1105
Cac8I GCNNGC 2 cut(s) 175, 359
CaiI CAGNNNCTG 1 cut(s) 1052
CciI TCATGA 3 cut(s) 778, 847, 964
Cfr42I CCGCGG 1 cut(s) 365
CseI GACGC 1 cut(s) 432
Csp6I GTAC 3 cut(s) 481, 609, 984
CviAII CATG 9 cut(s) 88, 235, 335, 779, 785, 841, 848, 965, 1030
CviQI GTAC 3 cut(s) 481, 609, 984
DdeI CTNAG 9 cut(s) 261, 498, 582, 603, 833, 915, 1195, 1243, 1252
DpnI GATC 9 cut(s) 101, 350, 470, 636, 678, 759, 825, 978, 1155
DpnII GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
Eco105I TACGTA 1 cut(s) 1183
Eco130I CCWWGG 2 cut(s) 784, 937
Eco32I GATATC 2 cut(s) 370, 881
Eco57I CTGAAG 3 cut(s) 262, 581, 1074
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 3 cut(s) 216, 905, 1233
EcoRII CCWGG 2 cut(s) 949, 979
EcoRV GATATC 2 cut(s) 370, 881
EcoT14I CCWWGG 2 cut(s) 784, 937
ErhI CCWWGG 2 cut(s) 784, 937
FaeI CATG 9 cut(s) 91, 238, 338, 782, 788, 844, 851, 968, 1033
FaqI GGGAC 3 cut(s) 498, 554, 651
FatI CATG 9 cut(s) 87, 234, 334, 778, 784, 840, 847, 964, 1029
FauI CCCGC 1 cut(s) 357
FauNDI CATATG 3 cut(s) 708, 862, 1038
FbaI TGATCA 2 cut(s) 676, 823
FokI GGATG 4 cut(s) 46, 96, 542, 1278
FspBI CTAG 5 cut(s) 510, 663, 749, 872, 1200
GsaI CCCAGC 1 cut(s) 1278
GsuI CTGGAG 1 cut(s) 699
HapII CCGG 3 cut(s) 83, 639, 669
HgaI GACGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 9 cut(s) 91, 238, 338, 782, 788, 844, 851, 968, 1033
HpaII CCGG 3 cut(s) 83, 639, 669
HphI GGTGA 6 cut(s) 800, 882, 926, 991, 1130, 1210
Hpy166II GTNNAC 2 cut(s) 791, 1121
Hpy188I TCNGA 7 cut(s) 104, 211, 244, 589, 724, 814, 1144
Hpy8I GTNNAC 2 cut(s) 791, 1121
HpyAV CCTTC 7 cut(s) 11, 17, 286, 421, 428, 436, 722
HpyCH4IV ACGT 1 cut(s) 1182
HpyCH4V TGCA 4 cut(s) 393, 698, 922, 996
HpyF10VI GCNNNNNNNGC 5 cut(s) 836, 1244, 1253, 1262, 1271
HpyF3I CTNAG 9 cut(s) 261, 498, 582, 603, 833, 915, 1195, 1243, 1252
HpySE526I ACGT 1 cut(s) 1182
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 9 cut(s) 91, 238, 338, 782, 788, 844, 851, 968, 1033
Ksp22I TGATCA 2 cut(s) 676, 823
KspI CCGCGG 1 cut(s) 365
Kzo9I GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
LmnI GCTCC 1 cut(s) 952
Lsp1109I GCAGC 7 cut(s) 82, 679, 682, 906, 980, 1009, 1012
LweI GCATC 2 cut(s) 931, 1005
MaeI CTAG 5 cut(s) 510, 663, 749, 872, 1200
MaeII ACGT 1 cut(s) 1182
MaeIII GTNAC 3 cut(s) 158, 325, 1048
MalI GATC 9 cut(s) 101, 350, 470, 636, 678, 759, 825, 978, 1155
MboI GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
MluCI AATT 6 cut(s) 221, 643, 819, 968, 1059, 1209
MlyI GAGTC 2 cut(s) 333, 706
MroXI GAANNNNTTC 2 cut(s) 383, 1059
MseI TTAA 3 cut(s) 407, 818, 1149
MslI CAYNNNNRTG 1 cut(s) 783
MspA1I CMGCKG 4 cut(s) 364, 919, 1160, 1274
MspI CCGG 3 cut(s) 83, 639, 669
MspR9I CCNGG 4 cut(s) 639, 669, 951, 981
MvaI CCWGG 2 cut(s) 951, 981
MvnI CGCG 1 cut(s) 364
MwoI GCNNNNNNNGC 5 cut(s) 836, 1244, 1253, 1262, 1271
NciI CCSGG 2 cut(s) 639, 669
NcoI CCATGG 1 cut(s) 784
NdeI CATATG 3 cut(s) 708, 862, 1038
NdeII GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
NlaIII CATG 9 cut(s) 91, 238, 338, 782, 788, 844, 851, 968, 1033
NlaIV GGNNCC 2 cut(s) 81, 912
NmuCI GTSAC 3 cut(s) 158, 325, 1048
NspV TTCGAA 1 cut(s) 429
PagI TCATGA 3 cut(s) 778, 847, 964
PdmI GAANNNNTTC 2 cut(s) 383, 1059
PfoI TCCNGGA 1 cut(s) 667
PleI GAGTC 2 cut(s) 332, 706
PpsI GAGTC 2 cut(s) 332, 706
Ppu21I YACGTR 1 cut(s) 1183
Psp6I CCWGG 2 cut(s) 949, 979
PspFI CCCAGC 1 cut(s) 1274
PspGI CCWGG 2 cut(s) 949, 979
PspN4I GGNNCC 2 cut(s) 81, 912
PstNI CAGNNNCTG 1 cut(s) 1052
PvuII CAGCTG 3 cut(s) 919, 1160, 1274
RsaI GTAC 3 cut(s) 482, 610, 985
RsaNI GTAC 3 cut(s) 481, 609, 984
RseI CAYNNNNRTG 1 cut(s) 783
SacII CCGCGG 1 cut(s) 365
SaqAI TTAA 3 cut(s) 407, 818, 1149
Sau3AI GATC 9 cut(s) 99, 348, 468, 634, 676, 757, 823, 976, 1153
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 2 cut(s) 333, 706
ScrFI CCNGG 4 cut(s) 639, 669, 951, 981
SfaNI GCATC 2 cut(s) 931, 1005
SfcI CTRYAG 2 cut(s) 958, 1161
Sfr303I CCGCGG 1 cut(s) 365
SfuI TTCGAA 1 cut(s) 429
SgrBI CCGCGG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 783
SnaBI TACGTA 1 cut(s) 1183
Sse9I AATT 6 cut(s) 221, 643, 819, 968, 1059, 1209
SsiI CCGC 5 cut(s) 69, 316, 362, 364, 1131
SspMI CTAG 5 cut(s) 510, 663, 749, 872, 1200
StyD4I CCNGG 4 cut(s) 637, 667, 949, 979
StyI CCWWGG 2 cut(s) 784, 937
TaiI ACGT 1 cut(s) 1185
TaqI TCGA 2 cut(s) 377, 429
TaqII GACCGA 1 cut(s) 604
TasI AATT 6 cut(s) 221, 643, 819, 968, 1059, 1209
TatI WGTACW 1 cut(s) 480
TauI GCSGC 3 cut(s) 318, 364, 1134
Tru1I TTAA 3 cut(s) 407, 818, 1149
Tru9I TTAA 3 cut(s) 407, 818, 1149
TscAI CASTG 4 cut(s) 121, 808, 1057, 1105
TseFI GTSAC 3 cut(s) 158, 325, 1048
TseI GCWGC 7 cut(s) 95, 692, 695, 919, 993, 1022, 1025
Tsp45I GTSAC 3 cut(s) 158, 325, 1048
TspDTI ATGAA 5 cut(s) 695, 836, 981, 1046, 1128
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 4 cut(s) 121, 808, 1057, 1105
XapI RAATTY 1 cut(s) 1059
XmnI GAANNNNTTC 2 cut(s) 383, 1059
XspI CTAG 5 cut(s) 510, 663, 749, 872, 1200
ZrmI AGTACT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.