MD01G1092800.v1.1
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007535
Physical Location & Seq
Reverse (-)
20755672 .. 20758434
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1092800.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGAGAAACATCAGCAGCAGCAGCAGCACCAACACCACCACAACCACCGCACAATCGGTGCCGGACATGGTGGCTGCGATCAGGTTCCATCCCACTGAAGATGAAAAGGTCGACTTCTTGAGCAAGAAGATGAAAAGTCACAACTCTCAAGCCTGCCTCTTCCTCCCTTTCATCGATGTCCGCAAGTTCGAGCCTTGGGAATTGCCTGAGCACATGTTCCCTGATTCTCCTCATCATGCTAAGGCGTGGTACTCCTTCAGCCCGTGTGGTTACAAATCCATCAATAGTCGTCGCTTCAATAGGGAAACAAAGAAAGGCTCTTGGAAGATGAACAGCAAGCAACGGGATGTGGGCTCGAAATATTTCACTTGCAAAAAGAGGACATTAACCTTCCAAATAAAGACCTTCCAGGAAGGTAGTAAGTCTAAATCCGTTCCGACCGGCTGGAAAATGCACGAGTACATTTGCATTAAACCTAAACGGGGTTCTAGTCCTCATCAGGAGAGGGATCAGCAAAGGGACCTTGTTCTCTGCGTCCTGAAGTATAAGCCAGTTGATTCTAAGTCTAATACGGGTACTTCGATCCGTGGTGATTTTGCTGATAGTACTGGTAATCGAGGCTACATTGCATCTAGTCCCGGAGATGATGAAGCTGCTACTGCTGCTGCAATGAATCAAGATGATGGTGAACCTGGTGGCGGCAGCAGCTCATCTGATTTTATTAATAAATCTCTATGCGACATGATTCAAGAGTGGCAGTCATGTGCTCCGGCAGTAGAATGTCTGAATTCATTCCTTCCTCTTCATGAACAACCACAGCTTGGACATTCTCTTGATATCTGTAGTCATGAATTGCTTGATCCTGGTACTGGTGGCTGCATCACCTATAGTTCTGATAACCAAGCTGCTGCAACGAATCATATTATTCCCGACACAGAAGAAATTCTTGCATCCAAACAGCTAGAAGGAAGTGCTGTGCTTGGCAGTGGCAATCATGATGATGGTGAACCTTGTAGCGGTATTTCATCTGATTTTAATGACCATGCAGTAGATGATATGCAGGAGTTTGATCAGCTAGACAAACTTTTGGATTTGCCCTTATCTCCTCCTGAGCTGTCTCAACCACACCAGCTGGAAAATGTTCCGGGCGTCCATAGTGGCAAATTCAGTAACTGGCCATCTCCAATTGGGGTTAATACTTCTTATGTTACTAATAAGAATAATATTGCAACCAATTGCGAAAACGAACCAGTTATCAACGTCGCTTTTAATTCTTATAATCGAGCGACAGATGAAAGGATTTCCGAGGTTTATTCTCAGGCAGAAGAAAATCTGAGTTTACCCTTTCAACCGTTTGATCAGCCTCAAAATTACACATGGCAGTCGCCAATGTTGTTCTCAGAAGTGGGAGAGCTTCTGCATGCCAATAACTATATTGGATGCAACGAGTCGCAATTTCTAGATATGGAGGTTGCGACTCTTTTCCCACAAAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

500

Amino Acids

55.41

Weight (kDa)

5.13

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 27 - 154 1.1e-21 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1278
AccB1I GGYRCC 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 821
AccI GTMKAC 1 cut(s) 111
AciI CCGC 4 cut(s) 48, 181, 699, 1017
AclWI GGATC 3 cut(s) 516, 577, 854
AcoI YGGCCR 1 cut(s) 1175
AcsI RAATTY 3 cut(s) 787, 942, 1163
AcuI CTGAAG 3 cut(s) 117, 241, 560
AcyI GRCGYC 1 cut(s) 1149
AfaI GTAC 5 cut(s) 251, 461, 577, 607, 868
AfiI CCNNNNNNNGG 5 cut(s) 482, 698, 821, 869, 1016
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 3 cut(s) 298, 749, 1349
AjnI CCWGG 3 cut(s) 408, 691, 862
AluBI AGCT 9 cut(s) 653, 708, 820, 905, 961, 1075, 1114, 1132, 1414
AluI AGCT 9 cut(s) 653, 708, 820, 905, 961, 1075, 1114, 1132, 1414
Alw21I GWGCWC 2 cut(s) 213, 769
Alw26I GTCTC 1 cut(s) 1122
AlwI GGATC 3 cut(s) 516, 577, 854
AlwNI CAGNNNCTG 1 cut(s) 1173
AoxI GGCC 1 cut(s) 1175
ApoI RAATTY 3 cut(s) 787, 942, 1163
AseI ATTAAT 1 cut(s) 723
Asp700I GAANNNNTTC 4 cut(s) 362, 791, 942, 1140
AspS9I GGNCC 1 cut(s) 520
AsuC2I CCSGG 2 cut(s) 639, 1146
AsuHPI GGTGA 4 cut(s) 602, 698, 874, 1016
AvaII GGWCC 1 cut(s) 520
BalI TGGCCA 1 cut(s) 1177
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 356
BauI CACGAG 1 cut(s) 455
Bbv12I GWGCWC 2 cut(s) 213, 769
BccI CCATC 5 cut(s) 96, 287, 677, 995, 1186
BciT130I CCWGG 3 cut(s) 410, 693, 864
BclI TGATCA 2 cut(s) 1069, 1357
BcnI CCSGG 2 cut(s) 639, 1146
BcoDI GTCTC 1 cut(s) 1122
BfaI CTAG 5 cut(s) 489, 633, 962, 1076, 1460
BfmI CTRYAG 2 cut(s) 841, 886
BmcAI AGTACT 1 cut(s) 607
Bme1390I CCNGG 5 cut(s) 410, 639, 693, 864, 1146
Bme18I GGWCC 1 cut(s) 520
BmgT120I GGNCC 1 cut(s) 520
BmiI GGNNCC 3 cut(s) 60, 86, 521
BmrFI CCNGG 5 cut(s) 410, 639, 693, 864, 1146
BmsI GCATC 4 cut(s) 638, 888, 959, 1430
Bpu10I CCTNAGC 3 cut(s) 207, 240, 1110
BpuEI CTTGAG 2 cut(s) 132, 139
BpuMI CCSGG 2 cut(s) 639, 1146
Bsa29I ATCGAT 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 1149
BsaJI CCNNGG 3 cut(s) 194, 586, 1305
Bsc4I CCNNNNNNNGG 5 cut(s) 482, 698, 821, 869, 1016
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 5 cut(s) 551, 613, 874, 1178, 1250
Bse3DI GCAATG 2 cut(s) 624, 675
BseBI CCWGG 3 cut(s) 410, 693, 864
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 194, 586, 1305
BseGI GGATG 4 cut(s) 88, 352, 950, 1445
BseLI CCNNNNNNNGG 5 cut(s) 482, 698, 821, 869, 1016
BseMI GCAATG 2 cut(s) 624, 675
BseMII CTCAG 5 cut(s) 198, 1101, 1325, 1331, 1413
BseNI ACTGG 5 cut(s) 551, 613, 874, 1178, 1250
BseRI GAGGAG 2 cut(s) 219, 1095
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 1 cut(s) 1177
BshNI GGYRCC 1 cut(s) 58
BshVI ATCGAT 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 441
BsiHKAI GWGCWC 2 cut(s) 213, 769
BsiSI CCGG 5 cut(s) 62, 441, 639, 770, 1145
BslFI GGGAC 2 cut(s) 533, 621
BslI CCNNNNNNNGG 5 cut(s) 482, 698, 821, 869, 1016
BsmAI GTCTC 1 cut(s) 1122
BsmFI GGGAC 2 cut(s) 533, 621
BsnI GGCC 1 cut(s) 1177
Bsp1286I GDGCHC 3 cut(s) 213, 356, 769
Bsp143I GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
BspACI CCGC 4 cut(s) 48, 181, 699, 1017
BspANI GGCC 1 cut(s) 1177
BspCNI CTCAG 5 cut(s) 199, 1102, 1326, 1330, 1412
BspDI ATCGAT 1 cut(s) 174
BspHI TCATGA 3 cut(s) 805, 847, 994
BspLI GGNNCC 3 cut(s) 60, 86, 521
BspPI GGATC 3 cut(s) 516, 577, 854
BspT107I GGYRCC 1 cut(s) 58
BsrDI GCAATG 2 cut(s) 624, 675
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 5 cut(s) 551, 613, 874, 1178, 1250
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 3 cut(s) 194, 586, 1305
BssMI GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
BssNI GRCGYC 1 cut(s) 1149
BssSI CACGAG 1 cut(s) 455
BssT1I CCWWGG 1 cut(s) 194
Bst2BI CACGAG 1 cut(s) 455
Bst2UI CCWGG 3 cut(s) 410, 693, 864
Bst4CI ACNGT 1 cut(s) 1353
Bst6I CTCTTC 2 cut(s) 164, 807
BstACI GRCGYC 1 cut(s) 1149
BstC8I GCNNGC 3 cut(s) 154, 338, 1422
BstDEI CTNAG 7 cut(s) 207, 240, 561, 1110, 1317, 1334, 1399
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 4 cut(s) 88, 352, 950, 1445
BstKTI GATC 6 cut(s) 81, 511, 585, 862, 1072, 1360
BstMAI GTCTC 1 cut(s) 1122
BstMBI GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 5 cut(s) 21, 24, 659, 662, 705
BstNI CCWGG 3 cut(s) 410, 693, 864
BstNSI RCATGY 2 cut(s) 217, 1424
BstSCI CCNGG 5 cut(s) 408, 637, 691, 862, 1144
BstSFI CTRYAG 2 cut(s) 841, 886
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 1 cut(s) 1177
BsuTUI ATCGAT 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 586
BtsCI GGATG 4 cut(s) 88, 352, 950, 1445
BtsI GCAGTG 1 cut(s) 991
BtsIMutI CAGTG 2 cut(s) 93, 991
Cac8I GCNNGC 3 cut(s) 154, 338, 1422
CaiI CAGNNNCTG 1 cut(s) 1173
CciI TCATGA 3 cut(s) 805, 847, 994
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 520
ClaI ATCGAT 1 cut(s) 174
CseI GACGC 2 cut(s) 523, 1138
CsiI ACCWGGT 1 cut(s) 691
Csp6I GTAC 5 cut(s) 250, 460, 576, 606, 867
CviQI GTAC 5 cut(s) 250, 460, 576, 606, 867
DdeI CTNAG 7 cut(s) 207, 240, 561, 1110, 1317, 1334, 1399
DpnI GATC 6 cut(s) 80, 510, 584, 861, 1071, 1359
DpnII GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
EaeI YGGCCR 1 cut(s) 1175
Eam1104I CTCTTC 2 cut(s) 164, 807
EarI CTCTTC 2 cut(s) 164, 807
Eco130I CCWWGG 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 356
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 520
Eco57I CTGAAG 3 cut(s) 117, 241, 560
EcoO109I RGGNCCY 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 3 cut(s) 408, 691, 862
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 194
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 194
FalI AAGNNNNNCTT 4 cut(s) 98, 130, 930, 962
FaqI GGGAC 2 cut(s) 533, 621
FbaI TGATCA 2 cut(s) 1069, 1357
FblI GTMKAC 1 cut(s) 111
FokI GGATG 4 cut(s) 75, 359, 937, 1452
FriOI GRGCYC 1 cut(s) 356
FspBI CTAG 5 cut(s) 489, 633, 962, 1076, 1460
HaeIII GGCC 1 cut(s) 1177
HapII CCGG 5 cut(s) 62, 441, 639, 770, 1145
HgaI GACGC 2 cut(s) 523, 1138
Hin1I GRCGYC 1 cut(s) 1149
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 7 cut(s) 224, 557, 673, 745, 916, 1448, 1477
HpaII CCGG 5 cut(s) 62, 441, 639, 770, 1145
HphI GGTGA 4 cut(s) 602, 698, 874, 1016
Hpy166II GTNNAC 4 cut(s) 112, 689, 1007, 1340
Hpy188I TCNGA 8 cut(s) 438, 715, 786, 895, 1030, 1306, 1335, 1402
Hpy8I GTNNAC 4 cut(s) 112, 689, 1007, 1340
Hpy99I CGWCG 2 cut(s) 294, 1265
HpyAV CCTTC 6 cut(s) 265, 400, 407, 415, 806, 959
HpyCH4III ACNGT 1 cut(s) 1353
HpyCH4IV ACGT 1 cut(s) 1260
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 24, 659, 662, 705
HpyF3I CTNAG 7 cut(s) 207, 240, 561, 1110, 1317, 1334, 1399
HpySE526I ACGT 1 cut(s) 1260
Hsp92I GRCGYC 1 cut(s) 1149
Ksp22I TGATCA 2 cut(s) 1069, 1357
Kzo9I GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
LmnI GCTCC 1 cut(s) 772
LweI GCATC 4 cut(s) 638, 888, 959, 1430
MabI ACCWGGT 1 cut(s) 691
MaeI CTAG 5 cut(s) 489, 633, 962, 1076, 1460
MaeII ACGT 1 cut(s) 1260
MaeIII GTNAC 4 cut(s) 137, 269, 1169, 1207
MalI GATC 6 cut(s) 80, 510, 584, 861, 1071, 1359
MboI GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
MboII GAAGA 7 cut(s) 110, 139, 151, 337, 794, 950, 1337
MfeI CAATTG 2 cut(s) 1185, 1234
MhlI GDGCHC 3 cut(s) 213, 356, 769
MlsI TGGCCA 1 cut(s) 1177
MluNI TGGCCA 1 cut(s) 1177
MlyI GAGTC 2 cut(s) 1457, 1471
MmeI TCCRAC 1 cut(s) 461
Mox20I TGGCCA 1 cut(s) 1177
MroXI GAANNNNTTC 4 cut(s) 362, 791, 942, 1140
MscI TGGCCA 1 cut(s) 1177
MseI TTAA 6 cut(s) 386, 471, 723, 1035, 1194, 1269
MslI CAYNNNNRTG 1 cut(s) 999
Msp20I TGGCCA 1 cut(s) 1177
MspA1I CMGCKG 1 cut(s) 1132
MspI CCGG 5 cut(s) 62, 441, 639, 770, 1145
MspR9I CCNGG 5 cut(s) 410, 639, 693, 864, 1146
MunI CAATTG 2 cut(s) 1185, 1234
MvaI CCWGG 3 cut(s) 410, 693, 864
MwoI GCNNNNNNNGC 5 cut(s) 21, 24, 659, 662, 705
NciI CCSGG 2 cut(s) 639, 1146
NdeII GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
NlaIV GGNNCC 3 cut(s) 60, 86, 521
NmuCI GTSAC 1 cut(s) 137
NspI RCATGY 2 cut(s) 217, 1424
PaeI GCATGC 1 cut(s) 1424
PagI TCATGA 3 cut(s) 805, 847, 994
PciI ACATGT 1 cut(s) 213
PcsI WCGNNNNNNNCGW 1 cut(s) 578
PdmI GAANNNNTTC 4 cut(s) 362, 791, 942, 1140
PfeI GAWTC 5 cut(s) 224, 557, 673, 745, 916
PflMI CCANNNNNTGG 1 cut(s) 821
PfoI TCCNGGA 2 cut(s) 408, 637
PleI GAGTC 2 cut(s) 1456, 1471
PpsI GAGTC 2 cut(s) 1456, 1471
PpuMI RGGWCCY 1 cut(s) 520
PscI ACATGT 1 cut(s) 213
PshBI ATTAAT 1 cut(s) 723
PsiI TTATAA 1 cut(s) 1278
Psp5II RGGWCCY 1 cut(s) 520
Psp6I CCWGG 3 cut(s) 408, 691, 862
PspGI CCWGG 3 cut(s) 408, 691, 862
PspN4I GGNNCC 3 cut(s) 60, 86, 521
PspPI GGNCC 1 cut(s) 520
PspPPI RGGWCCY 1 cut(s) 520
PstNI CAGNNNCTG 1 cut(s) 1173
PvuII CAGCTG 1 cut(s) 1132
RsaI GTAC 5 cut(s) 251, 461, 577, 607, 868
RsaNI GTAC 5 cut(s) 250, 460, 576, 606, 867
RseI CAYNNNNRTG 1 cut(s) 999
SalI GTCGAC 1 cut(s) 110
SaqAI TTAA 6 cut(s) 386, 471, 723, 1035, 1194, 1269
Sau3AI GATC 6 cut(s) 78, 508, 582, 859, 1069, 1357
Sau96I GGNCC 1 cut(s) 520
ScaI AGTACT 1 cut(s) 607
SchI GAGTC 2 cut(s) 1457, 1471
ScrFI CCNGG 5 cut(s) 410, 639, 693, 864, 1146
SduI GDGCHC 3 cut(s) 213, 356, 769
SexAI ACCWGGT 1 cut(s) 691
SfaNI GCATC 4 cut(s) 638, 888, 959, 1430
SfcI CTRYAG 2 cut(s) 841, 886
SinI GGWCC 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 2 cut(s) 118, 147
SmoI CTYRAG 2 cut(s) 118, 147
SphI GCATGC 1 cut(s) 1424
SsiI CCGC 4 cut(s) 48, 181, 699, 1017
SspI AATATT 2 cut(s) 362, 1225
SspMI CTAG 5 cut(s) 489, 633, 962, 1076, 1460
StyD4I CCNGG 5 cut(s) 408, 637, 691, 862, 1144
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 1 cut(s) 1353
TaiI ACGT 1 cut(s) 1263
TaqI TCGA 7 cut(s) 111, 174, 189, 356, 581, 616, 1282
TatI WGTACW 2 cut(s) 459, 605
TauI GCSGC 1 cut(s) 702
TfiI GAWTC 5 cut(s) 224, 557, 673, 745, 916
Tru1I TTAA 6 cut(s) 386, 471, 723, 1035, 1194, 1269
Tru9I TTAA 6 cut(s) 386, 471, 723, 1035, 1194, 1269
TscAI CASTG 2 cut(s) 100, 991
TseFI GTSAC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 137
TspGWI ACGGA 2 cut(s) 421, 575
TspRI CASTG 2 cut(s) 100, 991
Van91I CCANNNNNTGG 1 cut(s) 821
VpaK11BI GGWCC 1 cut(s) 520
VspI ATTAAT 1 cut(s) 723
XapI RAATTY 3 cut(s) 787, 942, 1163
XbaI TCTAGA 1 cut(s) 1459
XceI RCATGY 2 cut(s) 217, 1424
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 4 cut(s) 362, 791, 942, 1140
XspI CTAG 5 cut(s) 489, 633, 962, 1076, 1460
ZrmI AGTACT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.