MD01G1102900.v1.1

BLOC-1-related complex sub-unit 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006704
Physical Location & Seq
Forward (+)
21499731 .. 21501836
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1102900.v1.1.491

Sequence Viewer

Length: 789 bp
ATGCATGAATTCTCCACCACGGATGGCTTTATGGAGATAACTGAAGGCTTGGGAGAGATGATAAGCAACGTTGCAAACGAACCGTCAGTAGGGCTTTTTTATATTCAGCAGCACGTACAAAATGCAGCGCCCAATCTCGTTAGTCTTAGAAGTAATGTTGTGGAAAAGGCTCGTCAAACAGCTTTGCACACTGAGGATTCAGAGGATTCCATAGCTACGGTGAGATCAATGAAAGAATGTGGGGTCCCCGTTGCTGATGAGATGATTAAAGATATAAAGAAATCTCTAGCGCTTATGTCAACAAAGAAGCCGAAGAGAGGTGTAATCCATAAGCAAACATCAGGTTTTCAGATGGGAAGAACAAGCTCTTGGAAGCCAACAAGTTGGGTTCGTAACCCAAGCGATGCCCAACAGGATACCGAAAGAGGTTATTTTTCTACCGTAATCAAATCTGCCAGACAAAAAGCAAGTGGTTTCAAGTGGCCGCAACGTGATCCTGAAGAATTAACACAGACCTATTATTCTAACCCATCACTATCGGTTGCTTCTGCAAGCACCAGTTCATCTATGCCGGATGTGGAAGCTGAGGAGTTGCCCTTGTCGAGTCCTGTTGCTGACGAACCACAAGAAGAACAAGCTGATGAGAGCTTGTCGACTCCTCATTTATTACCAATGGCAGAAAACTATGATGAATTCAAGGCTGACAGAGAAAATAAACTAAAGGAGTGGTTGGAAGGAGCCGGTAACCTAGATAATCGCAGAGGAACAAGCGATGCGGCAAGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS8

Protein Analysis

263

Amino Acids

29.11

Weight (kDa)

5.04

Isoelectric Point (pI)

52.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS8 PF10167 4 - 112 3.5e-28 BLOC-1-related complex sub-unit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 653
AciI CCGC 2 cut(s) 485, 776
AclI AACGTT 1 cut(s) 69
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 2 cut(s) 8, 692
AcuI CTGAAG 2 cut(s) 63, 519
AfaI GTAC 1 cut(s) 117
AfeI AGCGCT 1 cut(s) 291
AfiI CCNNNNNNNGG 2 cut(s) 89, 317
AgsI TTSAA 2 cut(s) 478, 697
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
AluBI AGCT 6 cut(s) 182, 215, 366, 584, 638, 648
AluI AGCT 6 cut(s) 182, 215, 366, 584, 638, 648
AlwI GGATC 1 cut(s) 488
Aor51HI AGCGCT 1 cut(s) 291
AoxI GGCC 1 cut(s) 482
ApeKI GCWGC 2 cut(s) 109, 125
ApoI RAATTY 2 cut(s) 8, 692
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 2 cut(s) 130, 292
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 1 cut(s) 244
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 2 cut(s) 121, 137
BccI CCATC 3 cut(s) 17, 346, 538
BciVI GTATCC 1 cut(s) 409
BfaI CTAG 2 cut(s) 287, 749
BfoI RGCGCY 2 cut(s) 131, 293
BfuI GTATCC 1 cut(s) 409
BisI GCNGC 4 cut(s) 110, 126, 485, 777
BlsI GCNGC 4 cut(s) 111, 127, 486, 778
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 3 cut(s) 245, 246, 739
BmsI GCATC 2 cut(s) 394, 763
Bpu10I CCTNAGC 1 cut(s) 585
BsaAI YACGTR 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 18
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 317
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 1 cut(s) 558
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 2 cut(s) 28, 580
BseLI CCNNNNNNNGG 2 cut(s) 89, 317
BseMII CTCAG 2 cut(s) 183, 576
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 2 cut(s) 602, 648
BseXI GCAGC 2 cut(s) 121, 137
BshFI GGCC 1 cut(s) 484
BsiSI CCGG 2 cut(s) 572, 741
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 89, 317
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 1 cut(s) 484
Bsp143I GATC 2 cut(s) 224, 493
BspACI CCGC 2 cut(s) 485, 776
BspANI GGCC 1 cut(s) 484
BspCNI CTCAG 2 cut(s) 184, 577
BspLI GGNNCC 3 cut(s) 245, 246, 739
BspPI GGATC 1 cut(s) 488
BsrFI RCCGGY 1 cut(s) 740
BsrI ACTGG 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 2 cut(s) 224, 493
Bst4CI ACNGT 3 cut(s) 84, 220, 442
Bst6I CTCTTC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 3 cut(s) 146, 192, 585
BstDSI CCRYGG 1 cut(s) 18
BstEII GGTNACC 1 cut(s) 743
BstF5I GGATG 2 cut(s) 28, 580
BstH2I RGCGCY 2 cut(s) 131, 293
BstHHI GCGC 2 cut(s) 130, 292
BstKTI GATC 2 cut(s) 227, 496
BstMBI GATC 2 cut(s) 224, 493
BstPI GGTNACC 1 cut(s) 743
BstV1I GCAGC 2 cut(s) 121, 137
BstXI CCANNNNNNTGG 1 cut(s) 384
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 1 cut(s) 484
BtgI CCRYGG 1 cut(s) 18
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 2 cut(s) 28, 580
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 553
CfoI GCGC 2 cut(s) 130, 292
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 116
CviAII CATG 1 cut(s) 5
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 3 cut(s) 146, 192, 585
DpnI GATC 2 cut(s) 226, 495
DpnII GATC 2 cut(s) 224, 493
EaeI YGGCCR 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 244
Eco47III AGCGCT 1 cut(s) 291
Eco57I CTGAAG 2 cut(s) 63, 519
Eco91I GGTNACC 1 cut(s) 743
EcoO109I RGGNCCY 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 743
EcoRI GAATTC 2 cut(s) 8, 692
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 1 cut(s) 8
FaiI YATR 9 cut(s) 6, 32, 102, 212, 275, 296, 330, 569, 687
FaqI GGGAC 1 cut(s) 230
FatI CATG 1 cut(s) 4
FblI GTMKAC 1 cut(s) 653
Fnu4HI GCNGC 4 cut(s) 110, 126, 485, 777
FokI GGATG 2 cut(s) 35, 587
Fsp4HI GCNGC 4 cut(s) 110, 126, 485, 777
FspBI CTAG 2 cut(s) 287, 749
GlaI GCGC 2 cut(s) 129, 291
GluI GCNGC 4 cut(s) 110, 126, 485, 777
HaeII RGCGCY 2 cut(s) 131, 293
HaeIII GGCC 1 cut(s) 484
HapII CCGG 2 cut(s) 572, 741
HhaI GCGC 2 cut(s) 130, 292
Hin1II CATG 1 cut(s) 8
Hin6I GCGC 2 cut(s) 128, 290
HinP1I GCGC 2 cut(s) 128, 290
HincII GTYRAC 2 cut(s) 300, 654
HindII GTYRAC 2 cut(s) 300, 654
HinfI GANTC 4 cut(s) 197, 206, 604, 655
HpaII CCGG 2 cut(s) 572, 741
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 2 cut(s) 300, 654
Hpy188I TCNGA 2 cut(s) 202, 351
Hpy188III TCNNGA 1 cut(s) 497
Hpy8I GTNNAC 2 cut(s) 300, 654
HpyAV CCTTC 2 cut(s) 38, 728
HpyCH4III ACNGT 3 cut(s) 84, 220, 442
HpyCH4IV ACGT 3 cut(s) 69, 114, 490
HpyCH4V TGCA 5 cut(s) 4, 74, 125, 187, 551
HpyF3I CTNAG 3 cut(s) 146, 192, 585
HpySE526I ACGT 3 cut(s) 69, 114, 490
Hsp92II CATG 1 cut(s) 8
HspAI GCGC 2 cut(s) 128, 290
KflI GGGWCCC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 224, 493
LmnI GCTCC 1 cut(s) 737
LpnPI CCDG 8 cut(s) 327, 398, 469, 510, 571, 585, 621, 754
Lsp1109I GCAGC 2 cut(s) 121, 137
LweI GCATC 2 cut(s) 394, 763
MaeI CTAG 2 cut(s) 287, 749
MaeII ACGT 3 cut(s) 69, 114, 490
MaeIII GTNAC 2 cut(s) 392, 743
MalI GATC 2 cut(s) 226, 495
MboI GATC 2 cut(s) 224, 493
MboII GAAGA 4 cut(s) 325, 369, 512, 641
MluCI AATT 3 cut(s) 8, 503, 692
MlyI GAGTC 2 cut(s) 613, 649
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 7 cut(s) 187, 196, 311, 419, 580, 669, 755
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 267, 506
MspI CCGG 2 cut(s) 572, 741
NdeII GATC 2 cut(s) 224, 493
NlaIII CATG 1 cut(s) 8
NlaIV GGNNCC 3 cut(s) 245, 246, 739
NsiI ATGCAT 1 cut(s) 6
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 2 cut(s) 197, 206
PkrI GCNGC 4 cut(s) 111, 127, 486, 778
PleI GAGTC 2 cut(s) 612, 649
PpsI GAGTC 2 cut(s) 612, 649
Ppu21I YACGTR 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 244
Psp1406I AACGTT 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 244
PspEI GGTNACC 1 cut(s) 743
PspN4I GGNNCC 3 cut(s) 245, 246, 739
PspPI GGNCC 1 cut(s) 244
PspPPI RGGWCCY 1 cut(s) 244
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
SalI GTCGAC 1 cut(s) 652
SaqAI TTAA 2 cut(s) 267, 506
SatI GCNGC 4 cut(s) 110, 126, 485, 777
Sau3AI GATC 2 cut(s) 224, 493
Sau96I GGNCC 1 cut(s) 244
SchI GAGTC 2 cut(s) 613, 649
SfaNI GCATC 2 cut(s) 394, 763
SinI GGWCC 1 cut(s) 244
Sse9I AATT 3 cut(s) 8, 503, 692
SsiI CCGC 2 cut(s) 485, 776
SspMI CTAG 2 cut(s) 287, 749
TaaI ACNGT 3 cut(s) 84, 220, 442
TaiI ACGT 3 cut(s) 72, 117, 493
TaqI TCGA 2 cut(s) 602, 653
TasI AATT 3 cut(s) 8, 503, 692
TauI GCSGC 2 cut(s) 487, 779
TfiI GAWTC 2 cut(s) 197, 206
Tru1I TTAA 2 cut(s) 267, 506
Tru9I TTAA 2 cut(s) 267, 506
TscAI CASTG 1 cut(s) 196
TseI GCWGC 2 cut(s) 109, 125
TspDTI ATGAA 4 cut(s) 21, 245, 552, 705
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 244
XapI RAATTY 2 cut(s) 8, 692
XmiI GTMKAC 1 cut(s) 653
XspI CTAG 2 cut(s) 287, 749
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.