Rh1BG430500

BLOC-1-related complex sub-unit 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006704
Physical Location & Seq
Forward (+)
54673492 .. 54673896
405 bp
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UTR
Exon/CDS
Intron
Rh1BG430500.1

Sequence Viewer

Length: 405 bp
ATGCATGGATTCTCCACCGTCGACGGCTTTGTGGAGATAACTGAATGCTTGGGAGAGATGATTAAGTATGTGGCAAACGAACCCTCAGTGGGGCTTTATTATGTTCAGCAGCACACTCAAAATGCGGTGCCCAATCTCGTTGGTCTCAGAAATAATGTTGTGAACAAGTCGCGTGAAACAACTTTGCACACTGAAGATTCTGGGGATTCCATAACCATGGTGAGATCGATGAAAGAATGTGGATTCCCCGTTGCTGATGAGATGATTAGAGACATAAAGAAATCTCTTGTACTTTATGTCTACAAAACAACCAAAAAGAGGGCTAATCGACAACCAGGGATCAGCTTTTCAGCTGGGAAGAGGAAGTTCTTGGAAACCAGCCAGTTGGGGTCGTTCACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS8

Protein Analysis

134

Amino Acids

15.04

Weight (kDa)

9.12

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS8 PF10167 5 - 108 3e-27 BLOC-1-related complex sub-unit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AccI GTMKAC 2 cut(s) 21, 300
AccII CGCG 1 cut(s) 172
AciI CCGC 1 cut(s) 125
AclWI GGATC 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 402
AfaI GTAC 1 cut(s) 291
AfiI CCNNNNNNNGG 3 cut(s) 89, 90, 318
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 2 cut(s) 345, 353
AluI AGCT 2 cut(s) 345, 353
Alw26I GTCTC 2 cut(s) 149, 264
AlwI GGATC 1 cut(s) 347
ApeKI GCWGC 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 232, 388
BaeGI GKGCMC 1 cut(s) 132
BanI GGYRCC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 121
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 2 cut(s) 149, 264
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
Bme1390I CCNGG 1 cut(s) 336
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 336
Bsa29I ATCGAT 1 cut(s) 227
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 216, 335
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 90, 318
Bse1I ACTGG 1 cut(s) 382
BseBI CCWGG 1 cut(s) 336
BseCI ATCGAT 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 216, 335
BseLI CCNNNNNNNGG 3 cut(s) 89, 90, 318
BseMII CTCAG 2 cut(s) 99, 160
BseNI ACTGG 1 cut(s) 382
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 1 cut(s) 121
BseYI CCCAGC 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 127
BshVI ATCGAT 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 89, 90, 318
BsmAI GTCTC 2 cut(s) 149, 264
BsmI GAATGC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 2 cut(s) 224, 339
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 1 cut(s) 125
BspCNI CTCAG 2 cut(s) 98, 159
BspDI ATCGAT 1 cut(s) 227
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 1 cut(s) 347
BspT107I GGYRCC 1 cut(s) 127
BspTNI GGTCTC 1 cut(s) 149
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 2 cut(s) 216, 335
BssMI GATC 2 cut(s) 224, 339
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 353
BstDEI CTNAG 2 cut(s) 85, 146
BstDSI CCRYGG 1 cut(s) 216
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 2 cut(s) 227, 342
BstMAI GTCTC 2 cut(s) 149, 264
BstMBI GATC 2 cut(s) 224, 339
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 132
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 121
BstXI CCANNNNNNTGG 2 cut(s) 217, 385
Bsu15I ATCGAT 1 cut(s) 227
BsuTUI ATCGAT 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 93, 189
ClaI ATCGAT 1 cut(s) 227
Csp6I GTAC 1 cut(s) 290
CviAII CATG 2 cut(s) 5, 217
CviJI RGCY 6 cut(s) 27, 94, 323, 345, 353, 381
CviKI_1 RGCY 6 cut(s) 27, 94, 323, 345, 353, 381
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 2 cut(s) 85, 146
DpnI GATC 2 cut(s) 226, 341
DpnII GATC 2 cut(s) 224, 339
DraIII CACNNNGTG 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 216
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 8, 220
FaiI YATR 7 cut(s) 6, 69, 102, 212, 218, 275, 297
FatI CATG 2 cut(s) 4, 216
FblI GTMKAC 2 cut(s) 21, 300
Fnu4HI GCNGC 1 cut(s) 110
Fsp4HI GCNGC 1 cut(s) 110
GluI GCNGC 1 cut(s) 110
GsaI CCCAGC 1 cut(s) 357
Hin1II CATG 2 cut(s) 8, 220
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 4 cut(s) 9, 197, 206, 243
HphI GGTGA 2 cut(s) 232, 388
Hpy166II GTNNAC 4 cut(s) 22, 163, 301, 396
Hpy188I TCNGA 1 cut(s) 149
Hpy8I GTNNAC 4 cut(s) 22, 163, 301, 396
Hpy99I CGWCG 2 cut(s) 23, 26
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 2 cut(s) 4, 187
HpyF3I CTNAG 2 cut(s) 85, 146
Hsp92II CATG 2 cut(s) 8, 220
Kzo9I GATC 2 cut(s) 224, 339
LpnPI CCDG 6 cut(s) 186, 321, 339, 348, 391, 395
Lsp1109I GCAGC 1 cut(s) 121
MalI GATC 2 cut(s) 226, 341
MboI GATC 2 cut(s) 224, 339
MboII GAAGA 2 cut(s) 206, 370
MhlI GDGCHC 1 cut(s) 132
MnlI CCTC 3 cut(s) 94, 312, 354
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 172
NcoI CCATGG 1 cut(s) 216
NdeII GATC 2 cut(s) 224, 339
NlaIII CATG 2 cut(s) 8, 220
NlaIV GGNNCC 1 cut(s) 129
NsiI ATGCAT 1 cut(s) 6
PctI GAATGC 1 cut(s) 50
PfeI GAWTC 4 cut(s) 9, 197, 206, 243
PkrI GCNGC 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 215
SalI GTCGAC 1 cut(s) 20
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 2 cut(s) 224, 339
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 132
SetI ASST 2 cut(s) 347, 355
SgrDI CGTCGACG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 215
SsiI CCGC 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 3 cut(s) 21, 227, 328
TatI WGTACW 1 cut(s) 289
TfiI GAWTC 4 cut(s) 9, 197, 206, 243
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 2 cut(s) 93, 196
TseI GCWGC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 245
TspRI CASTG 2 cut(s) 93, 196
XmiI GTMKAC 2 cut(s) 21, 300
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.