pycom01g12940

BLOC-1-related complex sub-unit 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006704
Physical Location & Seq
Forward (+)
13534651 .. 13535442
792 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g12940.1

Sequence Viewer

Length: 792 bp
ATGCATGAATTCTCCACCGCGGATGGCTTTATGGAGATAACTGAAGGCTTGGGAGAGATGATTAGCTACGTTGCAAACGAACCGTCAGTAGGGCTTTTTTATATTCAGCAGCATGTACAAAATGCAGCGCCCAATCTCGTTAGTCTTAGAAGTAATGTTGTGGAAAAGTCTCGTCAAACAGCTTTGCACACTGAGGATTCAGAGGATTCCATAGCTACGGTGAGATCAATGAAAGAATGTGGGGTCCCCATTGCCGATGAGATGATTAAAGATATAAAGAAATCTCTAGCGCTTATGTCAACAAAGAAGCCGAAGAGAGGTGTAATCCATAAGCAAACATCAGGTTTTCAGATGGGAAGAACAAGCTCTTGGAAGCCAACAAGTTGGGTTCGTAACCCAAGCGATGCCCAACAGGATACTGAAAGAGGTTATTTTTCTAATGTGATCAAATCTGCCAGACAAAAAGCAAGTGGTTTCAAGTGGCCGCAACGTGATCCCGAAGAATTAACACAGACCTCTTATTCTAACCCATCACTATCGGTTGCTTCTGCAAGCACCAGTTCATCTATGCTGCCGGATGTAGAAGCTGAGGAGTTGCCCTTGTCGAGTCCTGTTGCTGACGAACCACAAGAAGAACAAGCTGATGAGAGCTTGTCGACTCCTCATTTATTACCAATGGCAGAAAACTATGATGAATTCAAGGCTGATAAAGAAAATAAGCTAAAGGAGTGGTTGGAAGGAGCCGGTAACCTAGATAATTGCAGAGCAACAAGCGATGCGGCAAGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS8

Protein Analysis

264

Amino Acids

29.14

Weight (kDa)

4.97

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS8 PF10167 4 - 112 4.9e-29 BLOC-1-related complex sub-unit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 656
AccII CGCG 1 cut(s) 20
AciI CCGC 4 cut(s) 18, 20, 485, 779
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 2 cut(s) 8, 695
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 117
AfeI AGCGCT 1 cut(s) 291
AfiI CCNNNNNNNGG 2 cut(s) 89, 317
AgsI TTSAA 2 cut(s) 478, 700
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
AluBI AGCT 8 cut(s) 66, 182, 215, 366, 587, 641, 651, 721
AluI AGCT 8 cut(s) 66, 182, 215, 366, 587, 641, 651, 721
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 1 cut(s) 488
Aor51HI AGCGCT 1 cut(s) 291
AoxI GGCC 1 cut(s) 482
ApeKI GCWGC 3 cut(s) 109, 125, 571
ApoI RAATTY 2 cut(s) 8, 695
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 2 cut(s) 130, 292
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 1 cut(s) 244
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 3 cut(s) 121, 137, 558
BccI CCATC 3 cut(s) 17, 346, 538
BciVI GTATCC 1 cut(s) 409
BclI TGATCA 1 cut(s) 444
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 2 cut(s) 287, 752
BfoI RGCGCY 2 cut(s) 131, 293
BfuI GTATCC 1 cut(s) 409
BisI GCNGC 5 cut(s) 110, 126, 485, 572, 780
BlsI GCNGC 5 cut(s) 111, 127, 486, 573, 781
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 3 cut(s) 245, 246, 742
BmsI GCATC 2 cut(s) 394, 766
Bpu10I CCTNAGC 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 18
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 317
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 2 cut(s) 28, 583
BseLI CCNNNNNNNGG 2 cut(s) 89, 317
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 183, 579
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 2 cut(s) 605, 651
BseXI GCAGC 3 cut(s) 121, 137, 558
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 1 cut(s) 484
BsiSI CCGG 2 cut(s) 575, 744
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 89, 317
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 1 cut(s) 484
Bsp1407I TGTACA 1 cut(s) 115
Bsp143I GATC 3 cut(s) 224, 444, 493
BspACI CCGC 4 cut(s) 18, 20, 485, 779
BspANI GGCC 1 cut(s) 484
BspCNI CTCAG 2 cut(s) 184, 580
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 3 cut(s) 245, 246, 742
BspPI GGATC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 115
BsrI ACTGG 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 3 cut(s) 224, 444, 493
Bst4CI ACNGT 2 cut(s) 84, 220
Bst6I CTCTTC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 3 cut(s) 146, 192, 588
BstDSI CCRYGG 1 cut(s) 18
BstEII GGTNACC 1 cut(s) 746
BstF5I GGATG 2 cut(s) 28, 583
BstFNI CGCG 1 cut(s) 20
BstH2I RGCGCY 2 cut(s) 131, 293
BstHHI GCGC 2 cut(s) 130, 292
BstKTI GATC 3 cut(s) 227, 447, 496
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 3 cut(s) 224, 444, 493
BstNSI RCATGY 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 746
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 3 cut(s) 121, 137, 558
BstXI CCANNNNNNTGG 1 cut(s) 384
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 1 cut(s) 484
BtgI CCRYGG 1 cut(s) 18
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 2 cut(s) 28, 583
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 553
CfoI GCGC 2 cut(s) 130, 292
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 1 cut(s) 244
Cfr42I CCGCGG 1 cut(s) 21
Csp6I GTAC 1 cut(s) 116
CviAII CATG 2 cut(s) 5, 113
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 3 cut(s) 146, 192, 588
DpnI GATC 3 cut(s) 226, 446, 495
DpnII GATC 3 cut(s) 224, 444, 493
EaeI YGGCCR 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 244
Eco47III AGCGCT 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 63
Eco91I GGTNACC 1 cut(s) 746
EcoO109I RGGNCCY 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 746
EcoRI GAATTC 2 cut(s) 8, 695
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 8, 116
FaqI GGGAC 1 cut(s) 230
FatI CATG 2 cut(s) 4, 112
FbaI TGATCA 1 cut(s) 444
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 5 cut(s) 110, 126, 485, 572, 780
FokI GGATG 2 cut(s) 35, 590
Fsp4HI GCNGC 5 cut(s) 110, 126, 485, 572, 780
FspBI CTAG 2 cut(s) 287, 752
GlaI GCGC 2 cut(s) 129, 291
GluI GCNGC 5 cut(s) 110, 126, 485, 572, 780
HaeII RGCGCY 2 cut(s) 131, 293
HaeIII GGCC 1 cut(s) 484
HapII CCGG 2 cut(s) 575, 744
HhaI GCGC 2 cut(s) 130, 292
Hin1II CATG 2 cut(s) 8, 116
Hin6I GCGC 2 cut(s) 128, 290
HinP1I GCGC 2 cut(s) 128, 290
HincII GTYRAC 2 cut(s) 300, 657
HindII GTYRAC 2 cut(s) 300, 657
HinfI GANTC 4 cut(s) 197, 206, 607, 658
HpaII CCGG 2 cut(s) 575, 744
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 2 cut(s) 300, 657
Hpy188I TCNGA 2 cut(s) 202, 351
Hpy188III TCNNGA 1 cut(s) 497
Hpy8I GTNNAC 2 cut(s) 300, 657
HpyAV CCTTC 2 cut(s) 38, 731
HpyCH4III ACNGT 2 cut(s) 84, 220
HpyCH4IV ACGT 2 cut(s) 69, 490
HpyCH4V TGCA 6 cut(s) 4, 74, 125, 187, 551, 762
HpyF3I CTNAG 3 cut(s) 146, 192, 588
HpySE526I ACGT 2 cut(s) 69, 490
Hsp92II CATG 2 cut(s) 8, 116
HspAI GCGC 2 cut(s) 128, 290
KflI GGGWCCC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 444
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 3 cut(s) 224, 444, 493
LmnI GCTCC 1 cut(s) 740
LpnPI CCDG 7 cut(s) 327, 398, 469, 571, 588, 624, 757
Lsp1109I GCAGC 3 cut(s) 121, 137, 558
LweI GCATC 2 cut(s) 394, 766
MaeI CTAG 2 cut(s) 287, 752
MaeII ACGT 2 cut(s) 69, 490
MaeIII GTNAC 2 cut(s) 392, 746
MalI GATC 3 cut(s) 226, 446, 495
MboI GATC 3 cut(s) 224, 444, 493
MboII GAAGA 4 cut(s) 325, 369, 512, 644
MluCI AATT 4 cut(s) 8, 503, 695, 757
MlyI GAGTC 2 cut(s) 616, 652
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 7 cut(s) 187, 196, 311, 419, 526, 583, 672
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 267, 506
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 2 cut(s) 575, 744
MvnI CGCG 1 cut(s) 20
NdeII GATC 3 cut(s) 224, 444, 493
NlaIII CATG 2 cut(s) 8, 116
NlaIV GGNNCC 3 cut(s) 245, 246, 742
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 2 cut(s) 197, 206
PkrI GCNGC 5 cut(s) 111, 127, 486, 573, 781
PleI GAGTC 2 cut(s) 615, 652
PpsI GAGTC 2 cut(s) 615, 652
PpuMI RGGWCCY 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 244
PspEI GGTNACC 1 cut(s) 746
PspN4I GGNNCC 3 cut(s) 245, 246, 742
PspPI GGNCC 1 cut(s) 244
PspPPI RGGWCCY 1 cut(s) 244
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
SacII CCGCGG 1 cut(s) 21
SalI GTCGAC 1 cut(s) 655
SaqAI TTAA 2 cut(s) 267, 506
SatI GCNGC 5 cut(s) 110, 126, 485, 572, 780
Sau3AI GATC 3 cut(s) 224, 444, 493
Sau96I GGNCC 1 cut(s) 244
SchI GAGTC 2 cut(s) 616, 652
SfaNI GCATC 2 cut(s) 394, 766
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 1 cut(s) 244
Sse9I AATT 4 cut(s) 8, 503, 695, 757
SsiI CCGC 4 cut(s) 18, 20, 485, 779
SspMI CTAG 2 cut(s) 287, 752
TaaI ACNGT 2 cut(s) 84, 220
TaiI ACGT 2 cut(s) 72, 493
TaqI TCGA 2 cut(s) 605, 656
TasI AATT 4 cut(s) 8, 503, 695, 757
TatI WGTACW 1 cut(s) 115
TauI GCSGC 2 cut(s) 487, 782
TfiI GAWTC 2 cut(s) 197, 206
Tru1I TTAA 2 cut(s) 267, 506
Tru9I TTAA 2 cut(s) 267, 506
TscAI CASTG 1 cut(s) 196
TseI GCWGC 3 cut(s) 109, 125, 571
TspDTI ATGAA 4 cut(s) 21, 245, 552, 708
TspRI CASTG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 244
XapI RAATTY 2 cut(s) 8, 695
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 656
XspI CTAG 2 cut(s) 287, 752
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.