pycom07g16340

BLOC-1-related complex sub-unit 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006704
Physical Location & Seq
Forward (+)
18750104 .. 18750892
789 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g16340.1

Sequence Viewer

Length: 789 bp
ATGCATGAGTTCTCCATCGTGGATGGCTTAGTGGAGATAACTGAAGGCTTGGGAGAGATGATTAGATACGTGGCAAATGAACCATCAGTAGGGCTTTTTTATATTCACCAACACACACAAAATGCAGTGCCTAATCTCGTTAGTCTTAGAAGTAATGTTGTGGAAAAGTCTTGTGAAACAGCTTTGCACACTGAGGATTCAGAGGATTCCATAGCTATGGTGAGATCAATGAAAGAATGTGGATTCCCCATTGCCAATGAGATGATTAGAGATATAAAGAAATCTCTAGTGCTTATGTCAACAAAGAAGCCGAAGAGAGATGTAATCCATCAGCAAACACCAGGTTTTCAGATGGGAAGAACAAGCTCTTGGAAGCCAACAAGTTGGGTTCGTTCCCTAGGTGATGCCCAACAGGATACTGAAAGCAATTATTTTTCTACCGTAATCAAATCTGCCAGACAAAAGGCGAGTAGTTTCAAGTGGCCGCAACTTGATCCTAAAGAATTAACACAGACCTCTAGTTCTAACCCATCACTATCAGTTGCTTTTGCAAGCACCAGTTCATCTGAGCCGGATGTAGAACTTGAGGAGTTGCCCTTGTCGAGTCATGTTGCTGACGAACAACAAGAAGAACAAGCCGATGGTAGCTTGTCAAGTCCTCATTTATTATCAATGGCAGAAAACTACGATGAATTCAAGGCTGATAAAGAAAATAATCTACAAGAGTGGTTGGAAGGGGCCGGTAATCTAGATAATCGCAGAGGAGCAAGCGATGCAGAAGGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS8

Protein Analysis

263

Amino Acids

29.13

Weight (kDa)

4.79

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS8 PF10167 5 - 109 8.2e-26 BLOC-1-related complex sub-unit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 485
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 1 cut(s) 692
AcuI CTGAAG 1 cut(s) 63
AfiI CCNNNNNNNGG 1 cut(s) 89
AgsI TTSAA 2 cut(s) 478, 697
AjnI CCWGG 1 cut(s) 340
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
AluBI AGCT 4 cut(s) 182, 215, 366, 648
AluI AGCT 4 cut(s) 182, 215, 366, 648
AlwI GGATC 1 cut(s) 488
AoxI GGCC 2 cut(s) 482, 738
ApoI RAATTY 1 cut(s) 692
AspA2I CCTAGG 1 cut(s) 397
AspS9I GGNCC 1 cut(s) 738
AsuHPI GGTGA 3 cut(s) 98, 232, 413
AvrII CCTAGG 1 cut(s) 397
BccI CCATC 7 cut(s) 17, 23, 91, 336, 346, 538, 635
BciT130I CCWGG 1 cut(s) 342
BciVI GTATCC 1 cut(s) 409
BfaI CTAG 4 cut(s) 287, 398, 519, 749
BfuI GTATCC 1 cut(s) 409
BisI GCNGC 1 cut(s) 485
BlnI CCTAGG 1 cut(s) 397
BlsI GCNGC 1 cut(s) 486
Bme1390I CCNGG 1 cut(s) 342
BmgT120I GGNCC 1 cut(s) 738
BmiI GGNNCC 1 cut(s) 739
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 2 cut(s) 394, 763
BpuEI CTTGAG 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 397
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 2 cut(s) 28, 580
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 183, 558
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 2 cut(s) 602, 777
BshFI GGCC 2 cut(s) 484, 740
BsiSI CCGG 2 cut(s) 572, 741
BslI CCNNNNNNNGG 1 cut(s) 89
BsnI GGCC 2 cut(s) 484, 740
Bsp143I GATC 2 cut(s) 224, 493
BspACI CCGC 1 cut(s) 485
BspANI GGCC 2 cut(s) 484, 740
BspCNI CTCAG 2 cut(s) 184, 559
BspLI GGNNCC 1 cut(s) 739
BspPI GGATC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 740
BsrI ACTGG 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 2 cut(s) 224, 493
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 308
BstAPI GCANNNNNTGC 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 2 cut(s) 553, 769
BstDEI CTNAG 4 cut(s) 28, 146, 192, 567
BstF5I GGATG 2 cut(s) 28, 580
BstKTI GATC 2 cut(s) 227, 496
BstMBI GATC 2 cut(s) 224, 493
BstMWI GCNNNNNNNGC 1 cut(s) 773
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstXI CCANNNNNNTGG 2 cut(s) 217, 384
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 2 cut(s) 484, 740
BtsCI GGATG 2 cut(s) 28, 580
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 132, 189
Cac8I GCNNGC 2 cut(s) 553, 769
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 1 cut(s) 738
CsiI ACCWGGT 1 cut(s) 340
CviAII CATG 2 cut(s) 5, 608
DdeI CTNAG 4 cut(s) 28, 146, 192, 567
DpnI GATC 2 cut(s) 226, 495
DpnII GATC 2 cut(s) 224, 493
EaeI YGGCCR 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 692
EcoRII CCWGG 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 397
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 2 cut(s) 8, 611
FaiI YATR 7 cut(s) 6, 102, 212, 218, 275, 296, 609
FatI CATG 2 cut(s) 4, 607
Fnu4HI GCNGC 1 cut(s) 485
FokI GGATG 2 cut(s) 35, 587
Fsp4HI GCNGC 1 cut(s) 485
FspBI CTAG 4 cut(s) 287, 398, 519, 749
GluI GCNGC 1 cut(s) 485
HaeIII GGCC 2 cut(s) 484, 740
HapII CCGG 2 cut(s) 572, 741
Hin1II CATG 2 cut(s) 8, 611
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 4 cut(s) 197, 206, 243, 604
HpaII CCGG 2 cut(s) 572, 741
HphI GGTGA 3 cut(s) 98, 232, 413
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 3 cut(s) 202, 351, 568
Hpy188III TCNNGA 1 cut(s) 749
Hpy8I GTNNAC 1 cut(s) 300
HpyAV CCTTC 3 cut(s) 38, 728, 773
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 5 cut(s) 4, 125, 187, 551, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 773
HpyF3I CTNAG 4 cut(s) 28, 146, 192, 567
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 2 cut(s) 8, 611
Kzo9I GATC 2 cut(s) 224, 493
LmnI GCTCC 1 cut(s) 764
LpnPI CCDG 7 cut(s) 327, 354, 398, 469, 571, 585, 754
LweI GCATC 2 cut(s) 394, 763
MabI ACCWGGT 1 cut(s) 340
MaeI CTAG 4 cut(s) 287, 398, 519, 749
MaeII ACGT 1 cut(s) 69
MalI GATC 2 cut(s) 226, 495
MboI GATC 2 cut(s) 224, 493
MboII GAAGA 3 cut(s) 325, 369, 641
MluCI AATT 3 cut(s) 427, 503, 692
MlyI GAGTC 1 cut(s) 613
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 6 cut(s) 187, 196, 526, 580, 669, 755
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 506
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 2 cut(s) 572, 741
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 773
NdeII GATC 2 cut(s) 224, 493
NlaIII CATG 2 cut(s) 8, 611
NlaIV GGNNCC 1 cut(s) 739
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 3 cut(s) 197, 206, 243
PkrI GCNGC 1 cut(s) 486
PleI GAGTC 1 cut(s) 612
PpsI GAGTC 1 cut(s) 612
Ppu21I YACGTR 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 1 cut(s) 739
PspPI GGNCC 1 cut(s) 738
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 1 cut(s) 506
SatI GCNGC 1 cut(s) 485
Sau3AI GATC 2 cut(s) 224, 493
Sau96I GGNCC 1 cut(s) 738
SchI GAGTC 1 cut(s) 613
ScrFI CCNGG 1 cut(s) 342
SetI ASST 8 cut(s) 72, 184, 217, 346, 368, 403, 518, 650
SexAI ACCWGGT 1 cut(s) 340
SfaNI GCATC 2 cut(s) 394, 763
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 3 cut(s) 427, 503, 692
SsiI CCGC 1 cut(s) 485
SspMI CTAG 4 cut(s) 287, 398, 519, 749
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 602
TasI AATT 3 cut(s) 427, 503, 692
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 3 cut(s) 197, 206, 243
Tru1I TTAA 1 cut(s) 506
Tru9I TTAA 1 cut(s) 506
TscAI CASTG 2 cut(s) 132, 196
TspDTI ATGAA 4 cut(s) 93, 245, 552, 705
TspRI CASTG 2 cut(s) 132, 196
XapI RAATTY 1 cut(s) 692
XbaI TCTAGA 1 cut(s) 748
XmaJI CCTAGG 1 cut(s) 397
XspI CTAG 4 cut(s) 287, 398, 519, 749
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.