RLG00000027640

BLOC-1-related complex sub-unit 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006704
Physical Location & Seq
Reverse (-)
13448342 .. 13449133
792 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027640

Sequence Viewer

Length: 792 bp
ATGCATGGATTCTCCACCGTCGACGGCTTTGTGGAGATAACTGAATGCTTGGGAGAGATGATTAAGTATGTGGCAAACGAACCCTCAGTGGGGCTTTATTATGTTCAGCAGCACACTCAAAATGCTGTGCCCAATCTCGTTGGCCTCAGAAATAATGTTGTGAACAAGTCGCGTGAAACAACTTTGCACACTGAAGATTCTGAGGATTCCATAACCATGGTGAGATCGATGAAAGAATGTGGATTCCCCGTTGCTGATGAGATGATTAGAGACATAAAGAAATCTCTTGTACTTATGTCTACAAAACAACCAAAAAGAGGGCTAATCGACAACCAGGGATCAGCTTTTCAGCTGGGAAGAGGAAGTTCTTGGAAACCAGCCAGTTGGGGTCGTTCACCAAGTGATGCCCAGCAGGATAGTGGAGGCAGTTATTTCTCCACTGTATTAAAATCTGCAAGACGAAAGGCCAGCAATTTGAAGTGGCCGCAACTTGATCCTAAAGAACTAACAGAGGTCGCCTCCTACTCTAACCCACCAGTATCAGTTGCTACTGCAAGCACTAGTTCGTCTCTGCCAGATACAGAAGCTGATGAGCTGCCCTTGTCGAGTCATATTACAGATGAGCCGCAAGAAGAACAAGTTGATGAGAGCTTGTCGACACATAATTTGTCTTCATTGTCAGAAAACTTTGATGAGTTCAAGGCTGATAAAGAAGTTAAACTACGAGAGTGGTTGGAAGGGACTGACAATCTAGTTAATCCCGGAGGAGCAAGTGATGCGGAAAGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS8

Protein Analysis

264

Amino Acids

28.92

Weight (kDa)

4.82

Isoelectric Point (pI)

54.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS8 PF10167 5 - 109 2.4e-28 BLOC-1-related complex sub-unit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 21, 299, 656
AccII CGCG 1 cut(s) 172
AciI CCGC 3 cut(s) 485, 626, 779
AclWI GGATC 2 cut(s) 346, 488
AcoI YGGCCR 1 cut(s) 482
AcuI CTGAAG 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 1 cut(s) 291
AfiI CCNNNNNNNGG 3 cut(s) 89, 90, 317
AgsI TTSAA 2 cut(s) 478, 700
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 5 cut(s) 344, 352, 587, 595, 651
AluI AGCT 5 cut(s) 344, 352, 587, 595, 651
Alw26I GTCTC 2 cut(s) 264, 573
AlwI GGATC 2 cut(s) 346, 488
AlwNI CAGNNNCTG 1 cut(s) 587
AoxI GGCC 3 cut(s) 142, 465, 482
ApeKI GCWGC 2 cut(s) 109, 595
ArsI GACNNNNNNTTYG 2 cut(s) 649, 681
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 2 cut(s) 232, 387
BaeGI GKGCMC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 705, 737
BbsI GAAGAC 1 cut(s) 663
BbvI GCAGC 2 cut(s) 121, 582
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 335
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 2 cut(s) 264, 573
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 2 cut(s) 561, 752
BglII AGATCT 1 cut(s) 785
BisI GCNGC 4 cut(s) 110, 485, 596, 626
BlsI GCNGC 4 cut(s) 111, 486, 597, 627
Bme1390I CCNGG 2 cut(s) 335, 762
BmrFI CCNGG 2 cut(s) 335, 762
BmsI GCATC 2 cut(s) 394, 766
BpiI GAAGAC 1 cut(s) 663
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpuMI CCSGG 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 216, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 90, 317
Bse1I ACTGG 2 cut(s) 381, 536
BseBI CCWGG 1 cut(s) 335
BseCI ATCGAT 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 216, 334
BseLI CCNNNNNNNGG 3 cut(s) 89, 90, 317
BseMII CTCAG 3 cut(s) 99, 160, 192
BseNI ACTGG 2 cut(s) 381, 536
BseRI GAGGAG 1 cut(s) 780
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 2 cut(s) 121, 582
BseYI CCCAGC 2 cut(s) 352, 408
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 3 cut(s) 144, 467, 484
BshVI ATCGAT 1 cut(s) 227
BsiSI CCGG 1 cut(s) 762
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 3 cut(s) 89, 90, 317
BsmAI GTCTC 2 cut(s) 264, 573
BsmBI CGTCTC 1 cut(s) 573
BsmFI GGGAC 1 cut(s) 754
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 3 cut(s) 144, 467, 484
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 4 cut(s) 224, 338, 493, 785
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 3 cut(s) 485, 626, 779
BspANI GGCC 3 cut(s) 144, 467, 484
BspCNI CTCAG 3 cut(s) 98, 159, 193
BspDI ATCGAT 1 cut(s) 227
BspFNI CGCG 1 cut(s) 172
BspPI GGATC 2 cut(s) 346, 488
BsrI ACTGG 2 cut(s) 381, 536
BssECI CCNNGG 2 cut(s) 216, 334
BssMI GATC 4 cut(s) 224, 338, 493, 785
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 19, 442
Bst6I CTCTTC 1 cut(s) 352
BstAPI GCANNNNNTGC 1 cut(s) 776
BstC8I GCNNGC 2 cut(s) 469, 556
BstDEI CTNAG 4 cut(s) 85, 146, 201, 789
BstDSI CCRYGG 1 cut(s) 216
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 4 cut(s) 227, 341, 496, 788
BstMAI GTCTC 2 cut(s) 264, 573
BstMBI GATC 4 cut(s) 224, 338, 493, 785
BstMWI GCNNNNNNNGC 1 cut(s) 776
BstNI CCWGG 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 333, 760
BstSLI GKGCMC 1 cut(s) 132
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 121, 582
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 1 cut(s) 785
BstXI CCANNNNNNTGG 2 cut(s) 217, 384
BstYI RGATCY 1 cut(s) 785
Bsu15I ATCGAT 1 cut(s) 227
BsuRI GGCC 3 cut(s) 144, 467, 484
BsuTUI ATCGAT 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 216
BtsIMutI CAGTG 3 cut(s) 93, 189, 438
Cac8I GCNNGC 2 cut(s) 469, 556
CaiI CAGNNNCTG 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 227
Csp6I GTAC 1 cut(s) 290
CviAII CATG 2 cut(s) 5, 217
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 4 cut(s) 85, 146, 201, 789
DpnI GATC 4 cut(s) 226, 340, 495, 787
DpnII GATC 4 cut(s) 224, 338, 493, 785
DraIII CACNNNGTG 1 cut(s) 401
EaeI YGGCCR 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 216
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 573
FaeI CATG 2 cut(s) 8, 220
FaiI YATR 9 cut(s) 6, 69, 102, 212, 218, 275, 296, 612, 663
FaqI GGGAC 1 cut(s) 754
FatI CATG 2 cut(s) 4, 216
FblI GTMKAC 3 cut(s) 21, 299, 656
Fnu4HI GCNGC 4 cut(s) 110, 485, 596, 626
Fsp4HI GCNGC 4 cut(s) 110, 485, 596, 626
FspBI CTAG 2 cut(s) 561, 752
GluI GCNGC 4 cut(s) 110, 485, 596, 626
GsaI CCCAGC 2 cut(s) 356, 412
HaeIII GGCC 3 cut(s) 144, 467, 484
HapII CCGG 1 cut(s) 762
Hin1II CATG 2 cut(s) 8, 220
HincII GTYRAC 2 cut(s) 22, 657
HindII GTYRAC 2 cut(s) 22, 657
HinfI GANTC 5 cut(s) 9, 197, 206, 243, 607
HpaII CCGG 1 cut(s) 762
HphI GGTGA 2 cut(s) 232, 387
Hpy166II GTNNAC 5 cut(s) 22, 163, 300, 395, 657
Hpy188I TCNGA 3 cut(s) 149, 202, 682
Hpy8I GTNNAC 5 cut(s) 22, 163, 300, 395, 657
Hpy99I CGWCG 2 cut(s) 23, 26
HpyAV CCTTC 1 cut(s) 731
HpyCH4III ACNGT 2 cut(s) 19, 442
HpyCH4V TGCA 4 cut(s) 4, 187, 455, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 776
HpyF3I CTNAG 4 cut(s) 85, 146, 201, 789
Hsp92II CATG 2 cut(s) 8, 220
Kzo9I GATC 4 cut(s) 224, 338, 493, 785
LmnI GCTCC 1 cut(s) 767
Lsp1109I GCAGC 2 cut(s) 121, 582
LweI GCATC 2 cut(s) 394, 766
MaeI CTAG 2 cut(s) 561, 752
MalI GATC 4 cut(s) 226, 340, 495, 787
MboI GATC 4 cut(s) 224, 338, 493, 785
MboII GAAGA 4 cut(s) 206, 369, 644, 663
MflI RGATCY 1 cut(s) 785
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 2 cut(s) 472, 664
MlyI GAGTC 1 cut(s) 616
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 9 cut(s) 94, 155, 196, 311, 353, 416, 505, 529, 758
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 4 cut(s) 63, 446, 717, 756
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 352
MspI CCGG 1 cut(s) 762
MspR9I CCNGG 2 cut(s) 335, 762
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 776
NciI CCSGG 1 cut(s) 762
NcoI CCATGG 1 cut(s) 216
NdeII GATC 4 cut(s) 224, 338, 493, 785
NlaIII CATG 2 cut(s) 8, 220
NsiI ATGCAT 1 cut(s) 6
PctI GAATGC 1 cut(s) 50
PfeI GAWTC 4 cut(s) 9, 197, 206, 243
PfoI TCCNGGA 1 cut(s) 760
PkrI GCNGC 4 cut(s) 111, 486, 597, 627
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 333
PspFI CCCAGC 2 cut(s) 352, 408
PspGI CCWGG 1 cut(s) 333
PstNI CAGNNNCTG 1 cut(s) 587
PsuI RGATCY 1 cut(s) 785
PvuII CAGCTG 1 cut(s) 352
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 215
SalI GTCGAC 2 cut(s) 20, 655
SaqAI TTAA 4 cut(s) 63, 446, 717, 756
SatI GCNGC 4 cut(s) 110, 485, 596, 626
Sau3AI GATC 4 cut(s) 224, 338, 493, 785
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 2 cut(s) 335, 762
SduI GDGCHC 1 cut(s) 132
SetI ASST 6 cut(s) 346, 354, 516, 589, 597, 653
SfaNI GCATC 2 cut(s) 394, 766
SgrDI CGTCGACG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 215
SpeI ACTAGT 1 cut(s) 560
Sse9I AATT 2 cut(s) 472, 664
SsiI CCGC 3 cut(s) 485, 626, 779
SspMI CTAG 2 cut(s) 561, 752
StyD4I CCNGG 2 cut(s) 333, 760
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 19, 442
TaqI TCGA 5 cut(s) 21, 227, 327, 605, 656
TasI AATT 2 cut(s) 472, 664
TatI WGTACW 1 cut(s) 289
TauI GCSGC 2 cut(s) 487, 628
TfiI GAWTC 4 cut(s) 9, 197, 206, 243
Tru1I TTAA 4 cut(s) 63, 446, 717, 756
Tru9I TTAA 4 cut(s) 63, 446, 717, 756
TscAI CASTG 3 cut(s) 93, 196, 445
TseI GCWGC 2 cut(s) 109, 595
TspDTI ATGAA 2 cut(s) 245, 663
TspRI CASTG 3 cut(s) 93, 196, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XmiI GTMKAC 3 cut(s) 21, 299, 656
XspI CTAG 2 cut(s) 561, 752
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.