MD01G1121500.v1.1

psbP-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010319
Physical Location & Seq
Reverse (-)
23511380 .. 23513553
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1121500.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGCAATTTCATCATCCATCTCATTGAGCTGCTGGGTTTCTACTGCCATACCCGACAACAAGTTTTACTGCAATGAGTTGCCACGAGCTACCTCGGCTCATTTTTCATGCAAATCAATCACTTGCTCAGGAGAGTCAGCCTCCATTGAAGAAAGCCATTGCAAGAGAAGGCCTCTACTTTTAGGATTTGGGGCACTAACCACAAGTCTACTTCATGCAAATTCCCTCTTTGCTCAAGAAATACCGGAAAAGTTTCGAGCTTTCGTCGACAAAGTAGATGGGTATTCATACTACTACCCCAACGATTGGAGGGACTTTGAGTTTAGAGCACATGACTCTGCATTCAAGGACAGATATATGCAGCTGCATAATGTTAGAGTGAGATTTATACCCACAAATAAAACTGACATCCATGATTTGGGTCCAATGGAACAGGTTGTTACCGATCTGGTGAGGCATAAACTTGCTGCACCAAACCAGTATGCAACTATATTTGGCGTCGAGGAGAAAAACATAGATGGGAAAAATTATTACACCATTGAGTATGGACTTGAATCTCCAAACTTTGCTACCACTTCATTCGCAACCATAGCCATTGGAAACGGGAGATACTACACCCTGATTGTCGGAGCAAACGAAAGACGGTGGAAAAGATACCGCAACCAGCTTAAAGTGGTAGCAGACTCTTTCAGAATGCTTGACATCTGA

Protein Analysis

236

Amino Acids

26.91

Weight (kDa)

8.21

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 84 - 231 2.2e-44 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 306, 418
AccI GTMKAC 2 cut(s) 208, 267
AciI CCGC 1 cut(s) 658
AcsI RAATTY 1 cut(s) 220
AcyI GRCGYC 1 cut(s) 498
AfiI CCNNNNNNNGG 2 cut(s) 306, 418
AgsI TTSAA 3 cut(s) 149, 346, 554
AluBI AGCT 5 cut(s) 30, 89, 260, 364, 667
AluI AGCT 5 cut(s) 30, 89, 260, 364, 667
Alw21I GWGCWC 1 cut(s) 331
AoxI GGCC 1 cut(s) 170
ApeKI GCWGC 4 cut(s) 30, 361, 364, 467
ApoI RAATTY 1 cut(s) 220
Asp700I GAANNNNTTC 1 cut(s) 252
AspS9I GGNCC 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 196
BauI CACGAG 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 17, 351, 373, 454
BccI CCATC 3 cut(s) 26, 272, 512
BisI GCNGC 4 cut(s) 31, 362, 365, 468
BlsI GCNGC 4 cut(s) 32, 363, 366, 469
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 423
BplI GAGNNNNNCTC 4 cut(s) 125, 157, 157, 189
Bpu10I CCTNAGC 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 498
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 418
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 2 cut(s) 79, 157
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 14, 408
BseLI CCNNNNNNNGG 2 cut(s) 306, 418
BseMI GCAATG 2 cut(s) 79, 157
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 196
BseXI GCAGC 4 cut(s) 17, 351, 373, 454
BseYI CCCAGC 1 cut(s) 33
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 306, 418
BsmFI GGGAC 1 cut(s) 326
BsmI GAATGC 2 cut(s) 341, 699
BsnI GGCC 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 196, 331
Bsp143I GATC 1 cut(s) 445
BspACI CCGC 1 cut(s) 658
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 1 cut(s) 140
BspLI GGNNCC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 79, 157
BsrI ACTGG 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 1 cut(s) 445
BssNI GRCGYC 1 cut(s) 498
BssSI CACGAG 1 cut(s) 84
Bst2BI CACGAG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 645
BstACI GRCGYC 1 cut(s) 498
BstDEI CTNAG 1 cut(s) 127
BstF5I GGATG 2 cut(s) 14, 408
BstKTI GATC 1 cut(s) 448
BstMBI GATC 1 cut(s) 445
BstMWI GCNNNNNNNGC 2 cut(s) 95, 590
BstSLI GKGCMC 1 cut(s) 196
BstV1I GCAGC 4 cut(s) 17, 351, 373, 454
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 2 cut(s) 14, 408
Cfr13I GGNCC 1 cut(s) 422
CseI GACGC 1 cut(s) 487
CviAII CATG 4 cut(s) 108, 215, 332, 413
DdeI CTNAG 1 cut(s) 127
DpnI GATC 1 cut(s) 447
DpnII GATC 1 cut(s) 445
Eco147I AGGCCT 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 422
FaeI CATG 4 cut(s) 111, 218, 335, 416
FaqI GGGAC 1 cut(s) 326
FatI CATG 4 cut(s) 107, 214, 331, 412
FblI GTMKAC 2 cut(s) 208, 267
Fnu4HI GCNGC 4 cut(s) 31, 362, 365, 468
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 4 cut(s) 31, 362, 365, 468
GluI GCNGC 4 cut(s) 31, 362, 365, 468
GsaI CCCAGC 1 cut(s) 37
HaeIII GGCC 1 cut(s) 172
HapII CCGG 1 cut(s) 245
HgaI GACGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 498
Hin1II CATG 4 cut(s) 111, 218, 335, 416
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 4 cut(s) 134, 335, 554, 683
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 2 cut(s) 209, 268
Hpy188I TCNGA 3 cut(s) 629, 692, 707
Hpy188III TCNNGA 2 cut(s) 129, 236
Hpy8I GTNNAC 2 cut(s) 209, 268
Hpy99I CGWCG 2 cut(s) 269, 503
HpyAV CCTTC 1 cut(s) 162
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4V TGCA 9 cut(s) 72, 111, 162, 218, 341, 361, 367, 470, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 590
HpyF3I CTNAG 1 cut(s) 127
Hsp92I GRCGYC 1 cut(s) 498
Hsp92II CATG 4 cut(s) 111, 218, 335, 416
Kzo9I GATC 1 cut(s) 445
LmnI GCTCC 1 cut(s) 629
LpnPI CCDG 8 cut(s) 19, 114, 258, 419, 434, 491, 632, 677
Lsp1109I GCAGC 4 cut(s) 17, 351, 373, 454
MaeIII GTNAC 1 cut(s) 439
MalI GATC 1 cut(s) 447
MboI GATC 1 cut(s) 445
MboII GAAGA 1 cut(s) 161
MhlI GDGCHC 2 cut(s) 196, 331
MluCI AATT 3 cut(s) 6, 220, 526
MlyI GAGTC 3 cut(s) 143, 329, 677
MmeI TCCRAC 1 cut(s) 607
MnlI CCTC 7 cut(s) 103, 151, 183, 236, 303, 447, 496
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 364
MspI CCGG 1 cut(s) 245
Mva1269I GAATGC 2 cut(s) 341, 699
MwoI GCNNNNNNNGC 2 cut(s) 95, 590
NdeII GATC 1 cut(s) 445
NlaIII CATG 4 cut(s) 111, 218, 335, 416
NlaIV GGNNCC 1 cut(s) 423
NmeAIII GCCGAG 1 cut(s) 74
PceI AGGCCT 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 633
PctI GAATGC 2 cut(s) 341, 699
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 1 cut(s) 554
PflMI CCANNNNNTGG 2 cut(s) 306, 418
PkrI GCNGC 4 cut(s) 32, 363, 366, 469
PleI GAGTC 3 cut(s) 142, 329, 677
PpsI GAGTC 3 cut(s) 142, 329, 677
PspFI CCCAGC 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 364
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 669
SatI GCNGC 4 cut(s) 31, 362, 365, 468
Sau3AI GATC 1 cut(s) 445
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 3 cut(s) 143, 329, 677
SduI GDGCHC 2 cut(s) 196, 331
SetI ASST 7 cut(s) 32, 91, 95, 262, 366, 438, 669
SinI GGWCC 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
Sse9I AATT 3 cut(s) 6, 220, 526
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 1 cut(s) 658
StuI AGGCCT 1 cut(s) 172
TaaI ACNGT 1 cut(s) 645
TaqI TCGA 3 cut(s) 256, 267, 501
TasI AATT 3 cut(s) 6, 220, 526
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 1 cut(s) 669
Tru9I TTAA 1 cut(s) 669
TseI GCWGC 4 cut(s) 30, 361, 364, 467
TspDTI ATGAA 4 cut(s) 96, 203, 276, 567
Van91I CCANNNNNTGG 2 cut(s) 306, 418
VpaK11BI GGWCC 1 cut(s) 422
XapI RAATTY 1 cut(s) 220
XmiI GTMKAC 2 cut(s) 208, 267
XmnI GAANNNNTTC 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.