Rw1G029550

psbP-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010319
Physical Location & Seq
Reverse (-)
57261165 .. 57263508
2344 bp
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UTR
Exon/CDS
Intron
Rw1G029550.1

Sequence Viewer

Length: 636 bp
ATGAGGTTCCGAAAGCTAACCGCTGCTTACTTTTCCGGTAAAACCATCACCTGCTCCAGTGAGACAGCTTCCAGTGAAGAAAGCTATTACAAGAGAAGGCCACTACTCTTGGGAGTTGGAGCACTAACTACAAGTCTACTTCATACAAGTTCCCTCTTGGCTGAAGGAATACCTGACAAGTTCCGAGGTTATGTTGACAAAGAAGATGGATATTCATATTATTACCCCTCTGATTGGAGGGATTTTGACTTCAGGGCACATGACTCTGCTTTCAAGGACCGATATATGCAACTTCACAATGTTAGAGTGAGATTTATACCCACAGACAAAAGTGACATCCATGATTTGGGTCCATTGGAAGAGGTTGTTCCCTATTTGGTGAGACACAAATTTGCTGCACCAAACCAAGTAGAAAAGATATTCGACATGCAGGAGAAAACTATAGATGGGAAGAGCTATTACACCTTCGAGTATGGACTCACATCTCCGAACTTCGCTACCACTTCCTTTGCGACCATAGCCATTGGAAATGGGAGATATTACACCCTAATCGTCGGAGCAAATGAGAGACGGTGGAAAAGATACCGCAACCAGCTTAAAATGGTTGCAGACTCTTTCAGAATGCTTGACATCTAA

Protein Analysis

211

Amino Acids

24.52

Weight (kDa)

8.98

Isoelectric Point (pI)

34.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 60 - 207 9.3e-44 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 346
AccI GTMKAC 1 cut(s) 136
AciI CCGC 2 cut(s) 21, 586
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 2 cut(s) 183, 235
AfiI CCNNNNNNNGG 2 cut(s) 234, 346
AgsI TTSAA 1 cut(s) 274
AluBI AGCT 5 cut(s) 16, 68, 84, 456, 595
AluI AGCT 5 cut(s) 16, 68, 84, 456, 595
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 3 cut(s) 56, 376, 562
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 23, 395
ApoI RAATTY 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 277, 350
AsuHPI GGTGA 2 cut(s) 40, 391
AvaII GGWCC 2 cut(s) 277, 350
BaeGI GKGCMC 1 cut(s) 259
BarI GAAGNNNNNNTAC 2 cut(s) 443, 475
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 10, 382
BccI CCATC 3 cut(s) 53, 200, 440
BcoDI GTCTC 3 cut(s) 56, 376, 562
BfmI CTRYAG 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 59
BisI GCNGC 2 cut(s) 24, 396
BlsI GCNGC 2 cut(s) 25, 397
Bme18I GGWCC 2 cut(s) 277, 350
BmgT120I GGNCC 2 cut(s) 277, 350
BmiI GGNNCC 2 cut(s) 8, 351
BpmI CTGGAG 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 346
Bse1I ACTGG 2 cut(s) 57, 72
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 234, 346
BseNI ACTGG 2 cut(s) 57, 72
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 2 cut(s) 10, 382
BsgI GTGCAG 1 cut(s) 381
BshFI GGCC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 124
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 234, 346
BsmAI GTCTC 3 cut(s) 56, 376, 562
BsmBI CGTCTC 1 cut(s) 562
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 1 cut(s) 100
Bsp1286I GDGCHC 2 cut(s) 124, 259
BspACI CCGC 2 cut(s) 21, 586
BspANI GGCC 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 8, 351
BspMI ACCTGC 1 cut(s) 59
BspQI GCTCTTC 1 cut(s) 446
BsrI ACTGG 2 cut(s) 57, 72
BssECI CCNNGG 1 cut(s) 184
Bst4CI ACNGT 1 cut(s) 573
Bst6I CTCTTC 2 cut(s) 354, 446
BstF5I GGATG 1 cut(s) 336
BstMAI GTCTC 3 cut(s) 56, 376, 562
BstMWI GCNNNNNNNGC 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 2 cut(s) 10, 382
BsuRI GGCC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 64, 79
BveI ACCTGC 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 277, 350
CspCI CAANNNNNGTGG 4 cut(s) 90, 125, 490, 525
CviAII CATG 3 cut(s) 260, 341, 427
CviJI RGCY 8 cut(s) 16, 68, 84, 100, 161, 456, 521, 595
CviKI_1 RGCY 8 cut(s) 16, 68, 84, 100, 161, 456, 521, 595
Eam1104I CTCTTC 2 cut(s) 354, 446
EarI CTCTTC 2 cut(s) 354, 446
Eco47I GGWCC 2 cut(s) 277, 350
Eco57I CTGAAG 2 cut(s) 183, 235
Esp3I CGTCTC 1 cut(s) 562
FaeI CATG 3 cut(s) 263, 344, 430
FatI CATG 3 cut(s) 259, 340, 426
FblI GTMKAC 1 cut(s) 136
Fnu4HI GCNGC 2 cut(s) 24, 396
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 24, 396
GluI GCNGC 2 cut(s) 24, 396
GsuI CTGGAG 1 cut(s) 40
HaeIII GGCC 1 cut(s) 100
HapII CCGG 1 cut(s) 36
Hin1II CATG 3 cut(s) 263, 344, 430
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 3 cut(s) 263, 477, 611
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 40, 391
Hpy166II GTNNAC 2 cut(s) 137, 196
Hpy188I TCNGA 6 cut(s) 11, 185, 232, 489, 557, 620
Hpy8I GTNNAC 2 cut(s) 137, 196
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 3 cut(s) 90, 158, 475
HpyCH4III ACNGT 1 cut(s) 573
HpyCH4V TGCA 4 cut(s) 289, 398, 430, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 518
Hsp92II CATG 3 cut(s) 263, 344, 430
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 3 cut(s) 59, 119, 557
LpnPI CCDG 8 cut(s) 49, 64, 70, 85, 186, 238, 416, 605
Lsp1109I GCAGC 2 cut(s) 10, 382
MaeIII GTNAC 1 cut(s) 332
MboII GAAGA 4 cut(s) 89, 215, 371, 463
MhlI GDGCHC 2 cut(s) 124, 259
MluCI AATT 1 cut(s) 389
MlyI GAGTC 3 cut(s) 257, 471, 605
MmeI TCCRAC 2 cut(s) 97, 535
MnlI CCTC 5 cut(s) 164, 179, 231, 238, 355
MseI TTAA 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 627
MwoI GCNNNNNNNGC 1 cut(s) 518
NlaIII CATG 3 cut(s) 263, 344, 430
NlaIV GGNNCC 2 cut(s) 8, 351
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 430
PaqCI CACCTGC 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 446
PctI GAATGC 1 cut(s) 627
PflMI CCANNNNNTGG 1 cut(s) 346
PkrI GCNGC 2 cut(s) 25, 397
PleI GAGTC 3 cut(s) 257, 471, 605
PpsI GAGTC 3 cut(s) 257, 471, 605
PspN4I GGNNCC 2 cut(s) 8, 351
PspPI GGNCC 2 cut(s) 277, 350
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 2 cut(s) 24, 396
Sau96I GGNCC 2 cut(s) 277, 350
SchI GAGTC 3 cut(s) 257, 471, 605
SduI GDGCHC 2 cut(s) 124, 259
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 2 cut(s) 277, 350
Sse9I AATT 1 cut(s) 389
SsiI CCGC 2 cut(s) 21, 586
TaaI ACNGT 1 cut(s) 573
TaqI TCGA 2 cut(s) 423, 468
TaqII GACCGA 1 cut(s) 294
TasI AATT 1 cut(s) 389
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TscAI CASTG 2 cut(s) 64, 79
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 23, 395
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 2 cut(s) 131, 204
TspRI CASTG 2 cut(s) 64, 79
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 277, 350
XapI RAATTY 1 cut(s) 389
XceI RCATGY 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.