MD01G1126700.v1.1

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007025
Physical Location & Seq
Forward (+)
23844944 .. 23846738
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1126700.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGTTTTCTCATCCGTTCCCGTCTATCTGGATCCACCCAACTGGCAACAACAGCAAACAAATCATCAACAAGGAGGTGGATGTGATCAGAATAATCTTCAGCTTCCGCAGCAGCCGTCTTCAGAACCTAGGTGCACTTCAAGAGATGGCAGCGGCAGTTTGGGCTCGATCAAACCTGGGTCCATGTCTGATCGAGCCCGGCTGGCCAATATACCACAGCCAGAGACGGCACCGAAATGCCCTCGATGTGAATCCACAAACACAAAGTTTTGCTACTTCAACAATTACAGCCTTTCCCAGCCTCGCCACTTTTGCAAGACGTGCCGGCGCTACTGGACCAGAGGAGGAGCCTTGAGAAGCGTGCCGGTGGGAGGAGGCTGCCGGCGAAACAAAAGAAGCAAAGAGGGCAACAGCAGCAGGTCAAAATCACCAGGCGCAGCTGGTAATTCAAGCTCAAGTACAGTCAATTCTTCCAACAGCTGCAGCACTAGTGATAATATCATAGGCCGTTTGATACATCCACCACCACCACCACATCAATTGCCCTTTTTGCCCTCCTTACACCATCTTACTGATTATGGTTCCGGGGACATGATAGGGTTGAATAATTTTGGGGGTATTAATCAACCCGGTGATGTTGAATTCCAACACCATCAGTACGGTTCGGATCGGTTGAGTGCCAGGCCAAATATTTTAGGCATGAATAATATGAGTATTTCTGAGCATTGGAGATCATCACTACTTCAGCAACAATTTCCTAATTTCTTGGCTAATTTGGAACCACCACCTAGTACTACTCATGGGATGTACCAATTGCCGTTAGATCATTCGGCGGGTAACAGAGTTACTACTACTCAGATGGTTAATGTGAAAATGGAACACAGTCATCAAGCACTGAATTTGTCAAGGAATTTTTCGGGAAGTCTGGGAAATGATCACCAATACTGGGGTACTAGTACTGCTGGTACTGGTGGCAGTGTTTGGACTGATCTTTCTGGTTTCACATCTTCTTCTTCTACCACCCATCTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

345

Amino Acids

37.54

Weight (kDa)

9.12

Isoelectric Point (pI)

58.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 77 - 132 7.6e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 408
AccB1I GGYRCC 1 cut(s) 229
AciI CCGC 3 cut(s) 107, 153, 833
AclWI GGATC 3 cut(s) 26, 39, 675
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 3 cut(s) 641, 898, 910
AcuI CTGAAG 3 cut(s) 83, 105, 728
AfaI GTAC 7 cut(s) 460, 659, 793, 809, 952, 958, 967
AfiI CCNNNNNNNGG 2 cut(s) 371, 946
AgsI TTSAA 5 cut(s) 141, 280, 450, 604, 641
AhlI ACTAGT 2 cut(s) 488, 953
AjiI CACGTC 1 cut(s) 321
AjnI CCWGG 3 cut(s) 175, 430, 680
AluBI AGCT 4 cut(s) 103, 440, 453, 480
AluI AGCT 4 cut(s) 103, 440, 453, 480
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 218
Alw44I GTGCAC 1 cut(s) 133
AlwI GGATC 3 cut(s) 26, 39, 675
AoxI GGCC 3 cut(s) 204, 505, 683
ApaLI GTGCAC 1 cut(s) 133
ApeKI GCWGC 8 cut(s) 109, 112, 150, 378, 414, 437, 480, 483
ApoI RAATTY 3 cut(s) 641, 898, 910
AseI ATTAAT 1 cut(s) 621
AspA2I CCTAGG 1 cut(s) 128
AspLEI GCGC 2 cut(s) 330, 437
AspS9I GGNCC 2 cut(s) 180, 336
AsuC2I CCSGG 3 cut(s) 199, 586, 630
AsuHPI GGTGA 3 cut(s) 420, 644, 929
AvaII GGWCC 2 cut(s) 180, 336
AvrII CCTAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 137
BalI TGGCCA 1 cut(s) 206
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 2 cut(s) 167, 199
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 137
BbvI GCAGC 8 cut(s) 121, 124, 162, 365, 426, 449, 467, 495
BccI CCATC 5 cut(s) 140, 573, 660, 853, 1032
BceAI ACGGC 4 cut(s) 100, 243, 492, 802
BciT130I CCWGG 3 cut(s) 177, 432, 682
BclI TGATCA 2 cut(s) 85, 934
BcnI CCSGG 3 cut(s) 199, 586, 630
BcoDI GTCTC 1 cut(s) 218
BcuI ACTAGT 2 cut(s) 488, 953
BfaI CTAG 4 cut(s) 129, 489, 789, 954
BfmI CTRYAG 1 cut(s) 481
BfoI RGCGCY 1 cut(s) 331
BfuAI ACCTGC 1 cut(s) 408
BglI GCCNNNNNGGC 1 cut(s) 203
BisI GCNGC 9 cut(s) 110, 113, 151, 154, 379, 415, 438, 481, 484
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 9 cut(s) 111, 114, 152, 155, 380, 416, 439, 482, 485
BmcAI AGTACT 2 cut(s) 793, 958
Bme1390I CCNGG 6 cut(s) 177, 199, 432, 586, 630, 682
Bme18I GGWCC 2 cut(s) 180, 336
BmgBI CACGTC 1 cut(s) 321
BmgT120I GGNCC 2 cut(s) 180, 336
BmiI GGNNCC 6 cut(s) 33, 181, 231, 349, 583, 780
BmrFI CCNGG 6 cut(s) 177, 199, 432, 586, 630, 682
BmrI ACTGGG 1 cut(s) 955
BmuI ACTGGG 1 cut(s) 955
BpiI GAAGAC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 373, 439
BpuMI CCSGG 3 cut(s) 199, 586, 630
BsaBI GATNNNNATC 1 cut(s) 250
BsaJI CCNNGG 3 cut(s) 128, 176, 585
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 946
Bse118I RCCGGY 3 cut(s) 324, 364, 381
Bse1I ACTGG 4 cut(s) 47, 338, 950, 973
Bse8I GATNNNNATC 1 cut(s) 250
BseBI CCWGG 3 cut(s) 177, 432, 682
BseDI CCNNGG 3 cut(s) 128, 176, 585
BseGI GGATG 4 cut(s) 11, 86, 517, 810
BseJI GATNNNNATC 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 371, 946
BseMII CTCAG 2 cut(s) 711, 869
BseNI ACTGG 4 cut(s) 47, 338, 950, 973
BseRI GAGGAG 3 cut(s) 357, 360, 387
BseSI GKGCMC 1 cut(s) 137
BseXI GCAGC 8 cut(s) 121, 124, 162, 365, 426, 449, 467, 495
BseYI CCCAGC 1 cut(s) 297
BshFI GGCC 3 cut(s) 206, 507, 685
BshNI GGYRCC 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 6 cut(s) 199, 325, 365, 382, 585, 630
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 371, 946
BsmAI GTCTC 1 cut(s) 218
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 3 cut(s) 206, 507, 685
Bsp1286I GDGCHC 3 cut(s) 137, 167, 199
Bsp143I GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
BspACI CCGC 3 cut(s) 107, 153, 833
BspANI GGCC 3 cut(s) 206, 507, 685
BspCNI CTCAG 2 cut(s) 712, 868
BspLI GGNNCC 6 cut(s) 33, 181, 231, 349, 583, 780
BspMAI CTGCAG 1 cut(s) 485
BspMI ACCTGC 1 cut(s) 408
BspPI GGATC 3 cut(s) 26, 39, 675
BspT107I GGYRCC 1 cut(s) 229
BsrFI RCCGGY 3 cut(s) 324, 364, 381
BsrI ACTGG 4 cut(s) 47, 338, 950, 973
BssAI RCCGGY 3 cut(s) 324, 364, 381
BssECI CCNNGG 3 cut(s) 128, 176, 585
BssMI GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
BssT1I CCWWGG 1 cut(s) 128
Bst2UI CCWGG 3 cut(s) 177, 432, 682
Bst4CI ACNGT 3 cut(s) 463, 662, 884
BstAPI GCANNNNNTGC 1 cut(s) 321
BstC8I GCNNGC 4 cut(s) 204, 326, 362, 383
BstDEI CTNAG 2 cut(s) 720, 855
BstF5I GGATG 4 cut(s) 11, 86, 517, 810
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 2 cut(s) 330, 437
BstKTI GATC 9 cut(s) 34, 88, 171, 193, 670, 734, 826, 937, 991
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
BstMWI GCNNNNNNNGC 9 cut(s) 52, 109, 162, 203, 312, 321, 405, 414, 550
BstNI CCWGG 3 cut(s) 177, 432, 682
BstSCI CCNGG 6 cut(s) 175, 197, 430, 584, 628, 680
BstSFI CTRYAG 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 137
BstV1I GCAGC 8 cut(s) 121, 124, 162, 365, 426, 449, 467, 495
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 3 cut(s) 206, 507, 685
BtrI CACGTC 1 cut(s) 321
BtsCI GGATG 4 cut(s) 11, 86, 517, 810
BtsI GCAGTG 1 cut(s) 982
BtsIMutI CAGTG 2 cut(s) 893, 982
BveI ACCTGC 1 cut(s) 408
Cac8I GCNNGC 4 cut(s) 204, 326, 362, 383
CfoI GCGC 2 cut(s) 330, 437
Cfr10I RCCGGY 3 cut(s) 324, 364, 381
Cfr13I GGNCC 2 cut(s) 180, 336
Csp6I GTAC 7 cut(s) 459, 658, 792, 808, 951, 957, 966
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviAII CATG 4 cut(s) 184, 592, 700, 800
CviQI GTAC 7 cut(s) 459, 658, 792, 808, 951, 957, 966
DdeI CTNAG 2 cut(s) 720, 855
DpnI GATC 9 cut(s) 33, 87, 170, 192, 669, 733, 825, 936, 990
DpnII GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
EaeI YGGCCR 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 128
Eco24I GRGCYC 2 cut(s) 167, 199
Eco47I GGWCC 2 cut(s) 180, 336
Eco57I CTGAAG 3 cut(s) 83, 105, 728
EcoRI GAATTC 1 cut(s) 641
EcoRII CCWGG 3 cut(s) 175, 430, 680
EcoT14I CCWWGG 1 cut(s) 128
EcoT38I GRGCYC 2 cut(s) 167, 199
ErhI CCWWGG 1 cut(s) 128
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 4 cut(s) 187, 595, 703, 803
FaiI YATR 8 cut(s) 185, 212, 503, 579, 593, 701, 710, 801
FaqI GGGAC 1 cut(s) 602
FatI CATG 4 cut(s) 183, 591, 699, 799
FauI CCCGC 1 cut(s) 826
FbaI TGATCA 2 cut(s) 85, 934
Fnu4HI GCNGC 9 cut(s) 110, 113, 151, 154, 379, 415, 438, 481, 484
FokI GGATG 3 cut(s) 93, 504, 817
FriOI GRGCYC 2 cut(s) 167, 199
Fsp4HI GCNGC 9 cut(s) 110, 113, 151, 154, 379, 415, 438, 481, 484
FspBI CTAG 4 cut(s) 129, 489, 789, 954
GlaI GCGC 2 cut(s) 329, 436
GluI GCNGC 9 cut(s) 110, 113, 151, 154, 379, 415, 438, 481, 484
GsaI CCCAGC 1 cut(s) 301
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 3 cut(s) 206, 507, 685
HapII CCGG 6 cut(s) 199, 325, 365, 382, 585, 630
HhaI GCGC 2 cut(s) 330, 437
Hin1II CATG 4 cut(s) 187, 595, 703, 803
Hin6I GCGC 2 cut(s) 328, 435
HinP1I GCGC 2 cut(s) 328, 435
HinfI GANTC 1 cut(s) 251
HpaII CCGG 6 cut(s) 199, 325, 365, 382, 585, 630
HphI GGTGA 3 cut(s) 420, 644, 929
Hpy166II GTNNAC 1 cut(s) 135
Hpy188I TCNGA 6 cut(s) 90, 124, 190, 667, 721, 858
Hpy188III TCNNGA 3 cut(s) 29, 141, 918
Hpy8I GTNNAC 1 cut(s) 135
HpyCH4III ACNGT 3 cut(s) 463, 662, 884
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 3 cut(s) 135, 315, 483
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 109, 162, 203, 312, 321, 405, 414, 550
HpyF3I CTNAG 2 cut(s) 720, 855
HpySE526I ACGT 1 cut(s) 320
Hsp92II CATG 4 cut(s) 187, 595, 703, 803
HspAI GCGC 2 cut(s) 328, 435
KroI GCCGGC 2 cut(s) 324, 381
KroNI GCCGGC 2 cut(s) 326, 383
Ksp22I TGATCA 2 cut(s) 85, 934
Kzo9I GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 8 cut(s) 121, 124, 162, 365, 426, 449, 467, 495
MaeI CTAG 4 cut(s) 129, 489, 789, 954
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 2 cut(s) 836, 844
MalI GATC 9 cut(s) 33, 87, 170, 192, 669, 733, 825, 936, 990
MboI GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
MboII GAAGA 6 cut(s) 89, 111, 462, 999, 1002, 1005
MfeI CAATTG 2 cut(s) 539, 812
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 3 cut(s) 137, 167, 199
MlsI TGGCCA 1 cut(s) 206
MluNI TGGCCA 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 498, 670
MnlI CCTC 9 cut(s) 68, 252, 312, 335, 338, 365, 368, 397, 565
Mox20I TGGCCA 1 cut(s) 206
MroNI GCCGGC 2 cut(s) 324, 381
MscI TGGCCA 1 cut(s) 206
MseI TTAA 2 cut(s) 621, 864
MslI CAYNNNNRTG 1 cut(s) 235
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 3 cut(s) 153, 440, 480
MspI CCGG 6 cut(s) 199, 325, 365, 382, 585, 630
MspR9I CCNGG 6 cut(s) 177, 199, 432, 586, 630, 682
MunI CAATTG 2 cut(s) 539, 812
MvaI CCWGG 3 cut(s) 177, 432, 682
MwoI GCNNNNNNNGC 9 cut(s) 52, 109, 162, 203, 312, 321, 405, 414, 550
NaeI GCCGGC 2 cut(s) 326, 383
NciI CCSGG 3 cut(s) 199, 586, 630
NdeII GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
NgoMIV GCCGGC 2 cut(s) 324, 381
NlaIII CATG 4 cut(s) 187, 595, 703, 803
NlaIV GGNNCC 6 cut(s) 33, 181, 231, 349, 583, 780
PdiI GCCGGC 2 cut(s) 326, 383
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 9 cut(s) 111, 114, 152, 155, 380, 416, 439, 482, 485
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 3 cut(s) 175, 430, 680
PspFI CCCAGC 1 cut(s) 297
PspGI CCWGG 3 cut(s) 175, 430, 680
PspN4I GGNNCC 6 cut(s) 33, 181, 231, 349, 583, 780
PspPI GGNCC 2 cut(s) 180, 336
PstI CTGCAG 1 cut(s) 485
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 440, 480
RsaI GTAC 7 cut(s) 460, 659, 793, 809, 952, 958, 967
RsaNI GTAC 7 cut(s) 459, 658, 792, 808, 951, 957, 966
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 2 cut(s) 621, 864
SatI GCNGC 9 cut(s) 110, 113, 151, 154, 379, 415, 438, 481, 484
Sau3AI GATC 9 cut(s) 31, 85, 168, 190, 667, 731, 823, 934, 988
Sau96I GGNCC 2 cut(s) 180, 336
ScaI AGTACT 2 cut(s) 793, 958
ScrFI CCNGG 6 cut(s) 177, 199, 432, 586, 630, 682
SduI GDGCHC 3 cut(s) 137, 167, 199
SfcI CTRYAG 1 cut(s) 481
SinI GGWCC 2 cut(s) 180, 336
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 352, 454
SmoI CTYRAG 2 cut(s) 352, 454
SpeI ACTAGT 2 cut(s) 488, 953
SsiI CCGC 3 cut(s) 107, 153, 833
SspI AATATT 1 cut(s) 691
SspMI CTAG 4 cut(s) 129, 489, 789, 954
StyD4I CCNGG 6 cut(s) 175, 197, 430, 584, 628, 680
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 3 cut(s) 463, 662, 884
TaiI ACGT 1 cut(s) 323
TaqI TCGA 3 cut(s) 167, 193, 244
TatI WGTACW 3 cut(s) 458, 791, 956
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 2 cut(s) 621, 864
Tru9I TTAA 2 cut(s) 621, 864
TscAI CASTG 2 cut(s) 900, 982
TseI GCWGC 8 cut(s) 109, 112, 150, 378, 414, 437, 480, 483
TspDTI ATGAA 1 cut(s) 716
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 2 cut(s) 900, 982
VneI GTGCAC 1 cut(s) 133
VpaK11BI GGWCC 2 cut(s) 180, 336
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 3 cut(s) 641, 898, 910
XmaJI CCTAGG 1 cut(s) 128
XspI CTAG 4 cut(s) 129, 489, 789, 954
ZrmI AGTACT 2 cut(s) 793, 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.