Rh1CG315400

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0007025
Physical Location & Seq
Forward (+)
58994975 .. 58997134
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG315400.1

Sequence Viewer

Length: 1104 bp
ATGGTTTTCTCATCCATTCCCGGCTATCTGGATCCACCCAACTGGCAGCAACAGCAATCCACTCATCATCATCAACAACAAGGAGGTGGCACGGATCAGAATCCCCATCTTCTTCCACCGCCACCACCACCTTCCGGAGGACCTGGAGATGCTGGTAACGGCGGAGCAGGCACGATTAGACCTGGATCGATGTCTGATCTTGCCAGACAGGCCAAGTTGCCGCAGCCCGAGACAGCCCTAAAATGCCCTCGATGCGAATCCACAAACACCAAGTTTTGCTACTTCAACAATTACAGCCTGACGCAGCCGCGCCACTTCTGCAAGACCTGTCGGCGCTACTGGACCCGAGGAGGAGCGCTGAGAAGTGTGCCGGTCGGAGGAGGCTGCAGAAGGAACAAAAGGAGCAAAAGGTCAAAGTCACCAGCGTCGGCATCAGCAGCAGATCATCATCATCAGCGCCAAGGCGGAGCTGGTTCGAGCTCCAGCACAGTTTCCAACTCCAACAGCTGCAGTACTAGTGAAAATATCTTAGGCCATTTGGCTCCTCCACCACATCAGTTTCCCTTTCTGCCCTCTTTACATCATCTAAGTGATTATAGTTCCGGCGGCTTAAGTTTCGGGGGGATTCAACAACCTTCTGGTGTTGAATTCCATAGTCAGAATATTGGTGCTAGTGGAAATAGTGGTGGTGTTGGGTCCATCTTGTCAACTGGGCTGGCTGAGCATTGGAGATCATCACTTCAGCATCATCAAGTTCAACAAGGGCAGCAATTTCCTTTCTTGGCTAATTTGGAGCCACCAAGTAGTCATGGCTTATACCAGTTTGAAGGTAATCAAAATAATGCTCACGATACAGCAAGCTATGGAAAAGGAGCTGGTCAGCTCCTTTCTAAGCCATTAGTGGATTCAAGCATTGGGGCTACTCAAGTGGCTAATGTGAAAATGGAAGAAAGCCAAGCATTGAATATGTCAAGAAATTTTTTGGGTAGTCTTGGAAATGATCATCATCAGCAATACTGGGGTGGTACTACTGCTGCTGGTGGCATTAATAATGCTTGGACTGATCTTTCAGGTTTCACTTCTTCTACCAGTCATCTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

367

Amino Acids

39.0

Weight (kDa)

8.98

Isoelectric Point (pI)

54.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 79 - 134 4.2e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1102
AccII CGCG 1 cut(s) 310
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 6 cut(s) 119, 162, 221, 308, 465, 606
AclWI GGATC 4 cut(s) 26, 39, 102, 193
AcsI RAATTY 2 cut(s) 647, 976
AcuI CTGAAG 1 cut(s) 725
AfaI GTAC 2 cut(s) 514, 1027
AfeI AGCGCT 1 cut(s) 357
AfiI CCNNNNNNNGG 3 cut(s) 134, 137, 377
AflII CTTAAG 1 cut(s) 610
AgsI TTSAA 7 cut(s) 286, 629, 647, 758, 827, 909, 964
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 2 cut(s) 142, 181
AluBI AGCT 6 cut(s) 470, 480, 507, 861, 875, 883
AluI AGCT 6 cut(s) 470, 480, 507, 861, 875, 883
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 4 cut(s) 26, 39, 102, 193
Ama87I CYCGRG 2 cut(s) 227, 345
Aor13HI TCCGGA 1 cut(s) 134
Aor51HI AGCGCT 1 cut(s) 357
AoxI GGCC 2 cut(s) 210, 532
ApeKI GCWGC 8 cut(s) 46, 223, 304, 384, 437, 507, 766, 1034
ApoI RAATTY 2 cut(s) 647, 976
AseI ATTAAT 1 cut(s) 1047
AspLEI GCGC 4 cut(s) 312, 336, 358, 459
AspS9I GGNCC 3 cut(s) 140, 342, 696
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 411
AvaI CYCGRG 2 cut(s) 227, 345
AvaII GGWCC 3 cut(s) 140, 342, 696
BamHI GGATCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 482
Bbv12I GWGCWC 1 cut(s) 482
BbvI GCAGC 8 cut(s) 58, 235, 316, 371, 449, 494, 778, 1021
BccI CCATC 2 cut(s) 114, 707
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 2 cut(s) 144, 183
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 224
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 2 cut(s) 516, 672
BfmI CTRYAG 2 cut(s) 385, 508
BfoI RGCGCY 3 cut(s) 337, 359, 460
BfrI CTTAAG 1 cut(s) 610
BglI GCCNNNNNGGC 1 cut(s) 209
BlpI GCTNAGC 1 cut(s) 720
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 3 cut(s) 21, 144, 183
Bme18I GGWCC 3 cut(s) 140, 342, 696
BmeT110I CYCGRG 2 cut(s) 227, 345
BmgT120I GGNCC 3 cut(s) 140, 342, 696
BmiI GGNNCC 5 cut(s) 33, 344, 543, 697, 795
BmrFI CCNGG 3 cut(s) 21, 144, 183
BmrI ACTGGG 2 cut(s) 720, 1027
BmsI GCATC 4 cut(s) 139, 242, 440, 754
BmuI ACTGGG 2 cut(s) 720, 1027
BpmI CTGGAG 2 cut(s) 165, 466
Bpu1102I GCTNAGC 1 cut(s) 720
BpuEI CTTGAG 1 cut(s) 909
BpuMI CCSGG 1 cut(s) 21
Bsa29I ATCGAT 1 cut(s) 188
BsaBI GATNNNNATC 4 cut(s) 99, 256, 447, 1005
BsaJI CCNNGG 2 cut(s) 346, 460
BsaWI WCCGGW 1 cut(s) 134
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 137, 377
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 6 cut(s) 47, 344, 715, 820, 1022, 1089
Bse8I GATNNNNATC 4 cut(s) 99, 256, 447, 1005
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 2 cut(s) 144, 183
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 2 cut(s) 346, 460
BseGI GGATG 1 cut(s) 11
BseJI GATNNNNATC 4 cut(s) 99, 256, 447, 1005
BseLI CCNNNNNNNGG 3 cut(s) 134, 137, 377
BseMII CTCAG 2 cut(s) 350, 711
BseNI ACTGG 6 cut(s) 47, 344, 715, 820, 1022, 1089
BseRI GAGGAG 4 cut(s) 363, 366, 393, 534
BseXI GCAGC 8 cut(s) 58, 235, 316, 371, 449, 494, 778, 1021
Bsh1236I CGCG 1 cut(s) 310
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 2 cut(s) 212, 534
BshVI ATCGAT 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 482
BsiHKCI CYCGRG 2 cut(s) 227, 345
BsiSI CCGG 4 cut(s) 21, 135, 371, 603
BslI CCNNNNNNNGG 3 cut(s) 134, 137, 377
BsmAI GTCTC 1 cut(s) 224
BsnI GGCC 2 cut(s) 212, 534
BsoBI CYCGRG 2 cut(s) 227, 345
Bsp1286I GDGCHC 1 cut(s) 482
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
Bsp1720I GCTNAGC 1 cut(s) 720
BspACI CCGC 6 cut(s) 119, 162, 221, 308, 465, 606
BspANI GGCC 2 cut(s) 212, 534
BspCNI CTCAG 2 cut(s) 351, 712
BspDI ATCGAT 1 cut(s) 188
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 1 cut(s) 310
BspLI GGNNCC 5 cut(s) 33, 344, 543, 697, 795
BspMAI CTGCAG 2 cut(s) 389, 512
BspPI GGATC 4 cut(s) 26, 39, 102, 193
BspTI CTTAAG 1 cut(s) 610
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 6 cut(s) 47, 344, 715, 820, 1022, 1089
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 346, 460
BssMI GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
BssT1I CCWWGG 1 cut(s) 460
Bst2UI CCWGG 2 cut(s) 144, 183
Bst4CI ACNGT 1 cut(s) 490
BstAFI CTTAAG 1 cut(s) 610
BstC8I GCNNGC 3 cut(s) 169, 717, 859
BstDEI CTNAG 5 cut(s) 359, 529, 587, 720, 891
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 11
BstFNI CGCG 1 cut(s) 310
BstH2I RGCGCY 3 cut(s) 337, 359, 460
BstHHI GCGC 4 cut(s) 312, 336, 358, 459
BstKTI GATC 8 cut(s) 34, 97, 188, 199, 445, 734, 1003, 1066
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 7 cut(s) 52, 168, 209, 252, 318, 437, 721
BstNI CCWGG 2 cut(s) 144, 183
BstSCI CCNGG 3 cut(s) 19, 142, 181
BstSFI CTRYAG 2 cut(s) 385, 508
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 8 cut(s) 58, 235, 316, 371, 449, 494, 778, 1021
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 188
BsuRI GGCC 2 cut(s) 212, 534
BsuTUI ATCGAT 1 cut(s) 188
BtsCI GGATG 1 cut(s) 11
Cac8I GCNNGC 3 cut(s) 169, 717, 859
CfoI GCGC 4 cut(s) 312, 336, 358, 459
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 3 cut(s) 140, 342, 696
ClaI ATCGAT 1 cut(s) 188
CseI GACGC 2 cut(s) 310, 414
Csp6I GTAC 2 cut(s) 513, 1026
CviAII CATG 1 cut(s) 809
CviQI GTAC 2 cut(s) 513, 1026
DdeI CTNAG 5 cut(s) 359, 529, 587, 720, 891
DpnI GATC 8 cut(s) 33, 96, 187, 198, 444, 733, 1002, 1065
DpnII GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
EciI GGCGGA 2 cut(s) 177, 480
Ecl136II GAGCTC 1 cut(s) 480
Eco130I CCWWGG 1 cut(s) 460
Eco24I GRGCYC 1 cut(s) 482
Eco47I GGWCC 3 cut(s) 140, 342, 696
Eco47III AGCGCT 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 725
Eco88I CYCGRG 2 cut(s) 227, 345
EcoICRI GAGCTC 1 cut(s) 480
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoO109I RGGNCCY 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 142, 181
EcoT14I CCWWGG 1 cut(s) 460
EcoT38I GRGCYC 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 460
FaeI CATG 1 cut(s) 812
FaiI YATR 7 cut(s) 597, 654, 810, 817, 864, 968, 1102
FatI CATG 1 cut(s) 808
FbaI TGATCA 1 cut(s) 1000
FriOI GRGCYC 1 cut(s) 482
FspBI CTAG 2 cut(s) 516, 672
GlaI GCGC 4 cut(s) 311, 335, 357, 458
GsuI CTGGAG 2 cut(s) 165, 466
HaeII RGCGCY 3 cut(s) 337, 359, 460
HaeIII GGCC 2 cut(s) 212, 534
HapII CCGG 4 cut(s) 21, 135, 371, 603
HgaI GACGC 2 cut(s) 310, 414
HhaI GCGC 4 cut(s) 312, 336, 358, 459
Hin1II CATG 1 cut(s) 812
Hin6I GCGC 4 cut(s) 310, 334, 356, 457
HinP1I GCGC 4 cut(s) 310, 334, 356, 457
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HinfI GANTC 4 cut(s) 100, 257, 625, 905
HpaII CCGG 4 cut(s) 21, 135, 371, 603
HphI GGTGA 1 cut(s) 411
Hpy166II GTNNAC 1 cut(s) 708
Hpy188I TCNGA 4 cut(s) 99, 196, 377, 660
Hpy188III TCNNGA 4 cut(s) 29, 135, 848, 972
Hpy8I GTNNAC 1 cut(s) 708
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 4 cut(s) 141, 384, 645, 821
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 3 cut(s) 321, 387, 510
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 168, 209, 252, 318, 437, 721
HpyF3I CTNAG 5 cut(s) 359, 529, 587, 720, 891
Hsp92II CATG 1 cut(s) 812
HspAI GCGC 4 cut(s) 310, 334, 356, 457
Kpn2I TCCGGA 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
LmnI GCTCC 9 cut(s) 164, 353, 402, 467, 485, 547, 793, 872, 888
Lsp1109I GCAGC 8 cut(s) 58, 235, 316, 371, 449, 494, 778, 1021
LweI GCATC 4 cut(s) 139, 242, 440, 754
MaeI CTAG 2 cut(s) 516, 672
MaeIII GTNAC 2 cut(s) 155, 417
MalI GATC 8 cut(s) 33, 96, 187, 198, 444, 733, 1002, 1065
MboI GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
MboII GAAGA 4 cut(s) 101, 104, 959, 1074
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 5 cut(s) 289, 647, 770, 787, 976
MmeI TCCRAC 3 cut(s) 355, 519, 525
MnlI CCTC 9 cut(s) 77, 131, 258, 341, 344, 371, 374, 555, 583
MroI TCCGGA 1 cut(s) 134
MseI TTAA 2 cut(s) 611, 1047
MslI CAYNNNNRTG 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 507
MspCI CTTAAG 1 cut(s) 610
MspI CCGG 4 cut(s) 21, 135, 371, 603
MspR9I CCNGG 3 cut(s) 21, 144, 183
MvaI CCWGG 2 cut(s) 144, 183
MvnI CGCG 1 cut(s) 310
MwoI GCNNNNNNNGC 7 cut(s) 52, 168, 209, 252, 318, 437, 721
NciI CCSGG 1 cut(s) 21
NdeII GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
NlaIII CATG 1 cut(s) 812
NlaIV GGNNCC 5 cut(s) 33, 344, 543, 697, 795
NmuCI GTSAC 1 cut(s) 417
PfeI GAWTC 4 cut(s) 100, 257, 625, 905
PpuMI RGGWCCY 1 cut(s) 140
PshBI ATTAAT 1 cut(s) 1047
PsiI TTATAA 1 cut(s) 1102
Psp124BI GAGCTC 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 142, 181
PspGI CCWGG 2 cut(s) 142, 181
PspN4I GGNNCC 5 cut(s) 33, 344, 543, 697, 795
PspPI GGNCC 3 cut(s) 140, 342, 696
PspPPI RGGWCCY 1 cut(s) 140
PstI CTGCAG 2 cut(s) 389, 512
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 507
RsaI GTAC 2 cut(s) 514, 1027
RsaNI GTAC 2 cut(s) 513, 1026
RseI CAYNNNNRTG 1 cut(s) 588
SacI GAGCTC 1 cut(s) 482
SaqAI TTAA 2 cut(s) 611, 1047
Sau3AI GATC 8 cut(s) 31, 94, 185, 196, 442, 731, 1000, 1063
Sau96I GGNCC 3 cut(s) 140, 342, 696
ScaI AGTACT 1 cut(s) 514
ScrFI CCNGG 3 cut(s) 21, 144, 183
SduI GDGCHC 1 cut(s) 482
SfaNI GCATC 4 cut(s) 139, 242, 440, 754
SfcI CTRYAG 2 cut(s) 385, 508
SinI GGWCC 3 cut(s) 140, 342, 696
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 2 cut(s) 610, 924
SmoI CTYRAG 2 cut(s) 610, 924
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 5 cut(s) 289, 647, 770, 787, 976
SsiI CCGC 6 cut(s) 119, 162, 221, 308, 465, 606
SspI AATATT 1 cut(s) 664
SspMI CTAG 2 cut(s) 516, 672
SstI GAGCTC 1 cut(s) 482
StyD4I CCNGG 3 cut(s) 19, 142, 181
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 3 cut(s) 188, 250, 476
TasI AATT 5 cut(s) 289, 647, 770, 787, 976
TatI WGTACW 1 cut(s) 512
TauI GCSGC 3 cut(s) 223, 310, 609
TfiI GAWTC 4 cut(s) 100, 257, 625, 905
Tru1I TTAA 2 cut(s) 611, 1047
Tru9I TTAA 2 cut(s) 611, 1047
TseFI GTSAC 1 cut(s) 417
TseI GCWGC 8 cut(s) 46, 223, 304, 384, 437, 507, 766, 1034
Tsp45I GTSAC 1 cut(s) 417
TspGWI ACGGA 1 cut(s) 107
Vha464I CTTAAG 1 cut(s) 610
VpaK11BI GGWCC 3 cut(s) 140, 342, 696
VspI ATTAAT 1 cut(s) 1047
XagI CCTNNNNNAGG 1 cut(s) 135
XapI RAATTY 2 cut(s) 647, 976
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
XspI CTAG 2 cut(s) 516, 672
ZrmI AGTACT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.