MD07G1197500.v1.1

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007025
Physical Location & Seq
Forward (+)
27551969 .. 27553842
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1197500.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGTTTTCTCATCCGTTCCCGTCTATCTGGATCCACCCAACTGGCAACAACAGCAAACAAATCATCATCAACAAGGAGGTGGAAGTGATCAGAATAATCCTCAGCTTCCATTGGTGCCTTCTTCAGAAGCTAGATGCACTTCTGTAGATGGCGGCGGTGGTTTGGGCTCGATTAAACCTGGATCGATGTGTGATCGAGCCCGGATGGCCAAGCTGCCGCAGCCAGAGACAGCACTGAAATGCCCTCGATGTGAATCCACAAACACAAAGTTTTGCTACTTCAACAATTACAACCTTTCCCAGCCTCGCCACTTTTGCAAGACGTGCAGGCGCTACTGGACAAGAGGAGGAGCCTTGAGAAGCGTGCCGGTGGGAGGAGGCTGCCGGAGAAATAAAAGAAGCAAAGGTGGCAACAGCAGCAGGTCAAAATCACCAGGCGCAGCTGGTAATTCTAGCTCAAGTACGGTTAATTCTTCCAACAGCTGCAGCGCTAGTGATAATATCATAGGCCATTTGACACATCCACCACCACCACATCAATTGCCGTTTTTGCCCTCCTTACACCATCTTCCTGATTATGGTTCCGGGGACATGCTAGGGCTAAATAATTTTGGGATTAATCAACCTGGTGATGTTGAATTCCAACACCATCAGTACGGTTCGGACCGGTTCGGTGCAAGGCCAAATATTTTAGGCATGAATAATATGAGTAGTACTGAGCATTGGAGATCATCACTACTTCAACAGCAATTTCCTAATTTCTTGGTTAATTTGGGACAACCAACTAGTACTACTCATGGGTTGTACCAATTTGACGGTGGAGATCATCAAAATGTTGTCCAGCTCCCGATATCTAAGCCGTTGGATCATTCTGCGGGTAACGGAGTTACTACTACTCAGATGGCTAATGTGAAAATGGAACAGAATCAAGCACTGAATTTGTCAAGGAATTTTTTGGGAAGCCTTGGAAATGATCACCAATACTGGGGTAGTAGTACTACTGGTACTGGTGGGAATGCTTGGACTGATCTTTCTGGTTTCACAACTTCTTCTTCTACCAGCCATCTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

358

Amino Acids

38.64

Weight (kDa)

8.99

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 78 - 133 2.1e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 115
AciI CCGC 4 cut(s) 153, 156, 218, 875
AclWI GGATC 4 cut(s) 26, 39, 190, 873
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 3 cut(s) 638, 937, 949
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 7 cut(s) 463, 656, 715, 790, 806, 997, 1006
AfeI AGCGCT 1 cut(s) 490
AfiI CCNNNNNNNGG 3 cut(s) 374, 578, 985
AgeI ACCGGT 1 cut(s) 666
AgsI TTSAA 3 cut(s) 283, 638, 743
AhlI ACTAGT 1 cut(s) 785
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 3 cut(s) 178, 433, 625
AluBI AGCT 7 cut(s) 106, 131, 214, 443, 456, 483, 844
AluI AGCT 7 cut(s) 106, 131, 214, 443, 456, 483, 844
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 4 cut(s) 26, 39, 190, 873
Aor51HI AGCGCT 1 cut(s) 490
AoxI GGCC 3 cut(s) 207, 508, 680
ApeKI GCWGC 7 cut(s) 214, 220, 381, 417, 440, 483, 486
ApoI RAATTY 3 cut(s) 638, 937, 949
AseI ATTAAT 1 cut(s) 618
AsiGI ACCGGT 1 cut(s) 666
AspLEI GCGC 3 cut(s) 333, 440, 491
AspS9I GGNCC 1 cut(s) 664
AsuC2I CCSGG 2 cut(s) 202, 586
AsuHPI GGTGA 3 cut(s) 423, 641, 968
AvaII GGWCC 1 cut(s) 664
BalI TGGCCA 1 cut(s) 209
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 2 cut(s) 170, 202
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 7 cut(s) 201, 232, 368, 429, 452, 470, 498
BccI CCATC 6 cut(s) 143, 199, 573, 657, 895, 1071
BceAI ACGGC 2 cut(s) 529, 844
BciT130I CCWGG 3 cut(s) 180, 435, 627
BclI TGATCA 2 cut(s) 88, 973
BcnI CCSGG 2 cut(s) 202, 586
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 5 cut(s) 132, 453, 492, 596, 786
BfmI CTRYAG 2 cut(s) 144, 484
BfoI RGCGCY 2 cut(s) 334, 492
BfuAI ACCTGC 1 cut(s) 411
BglI GCCNNNNNGGC 1 cut(s) 206
BisI GCNGC 9 cut(s) 154, 215, 218, 221, 382, 418, 441, 484, 487
BlsI GCNGC 9 cut(s) 155, 216, 219, 222, 383, 419, 442, 485, 488
BmcAI AGTACT 3 cut(s) 715, 790, 997
Bme1390I CCNGG 5 cut(s) 180, 202, 435, 586, 627
Bme18I GGWCC 1 cut(s) 664
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 664
BmiI GGNNCC 4 cut(s) 33, 117, 352, 583
BmrFI CCNGG 5 cut(s) 180, 202, 435, 586, 627
BmrI ACTGGG 1 cut(s) 994
BmsI GCATC 1 cut(s) 125
BmuI ACTGGG 1 cut(s) 994
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 376, 442
BpuMI CCSGG 2 cut(s) 202, 586
Bsa29I ATCGAT 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 253
BsaJI CCNNGG 2 cut(s) 585, 964
BsaWI WCCGGW 1 cut(s) 666
Bsc4I CCNNNNNNNGG 3 cut(s) 374, 578, 985
Bse118I RCCGGY 2 cut(s) 367, 666
Bse1I ACTGG 5 cut(s) 47, 341, 989, 1006, 1012
Bse8I GATNNNNATC 1 cut(s) 253
BseBI CCWGG 3 cut(s) 180, 435, 627
BseCI ATCGAT 1 cut(s) 185
BseDI CCNNGG 2 cut(s) 585, 964
BseGI GGATG 3 cut(s) 11, 210, 520
BseJI GATNNNNATC 1 cut(s) 253
BseLI CCNNNNNNNGG 3 cut(s) 374, 578, 985
BseMII CTCAG 3 cut(s) 116, 708, 911
BseNI ACTGG 5 cut(s) 47, 341, 989, 1006, 1012
BseRI GAGGAG 3 cut(s) 360, 363, 390
BseXI GCAGC 7 cut(s) 201, 232, 368, 429, 452, 470, 498
BseYI CCCAGC 1 cut(s) 300
BsgI GTGCAG 1 cut(s) 346
BshFI GGCC 3 cut(s) 209, 510, 682
BshNI GGYRCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 666
BshVI ATCGAT 1 cut(s) 185
BsiSI CCGG 5 cut(s) 202, 368, 385, 585, 667
BslFI GGGAC 2 cut(s) 602, 789
BslI CCNNNNNNNGG 3 cut(s) 374, 578, 985
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 602, 789
BsmI GAATGC 1 cut(s) 1021
BsnI GGCC 3 cut(s) 209, 510, 682
Bsp1286I GDGCHC 2 cut(s) 170, 202
Bsp143I GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
BspACI CCGC 4 cut(s) 153, 156, 218, 875
BspANI GGCC 3 cut(s) 209, 510, 682
BspCNI CTCAG 3 cut(s) 115, 709, 910
BspDI ATCGAT 1 cut(s) 185
BspLI GGNNCC 4 cut(s) 33, 117, 352, 583
BspMAI CTGCAG 1 cut(s) 488
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 4 cut(s) 26, 39, 190, 873
BspT107I GGYRCC 1 cut(s) 115
BsrFI RCCGGY 2 cut(s) 367, 666
BsrI ACTGG 5 cut(s) 47, 341, 989, 1006, 1012
BssAI RCCGGY 2 cut(s) 367, 666
BssECI CCNNGG 2 cut(s) 585, 964
BssMI GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
BssT1I CCWWGG 1 cut(s) 964
Bst2UI CCWGG 3 cut(s) 180, 435, 627
Bst4CI ACNGT 3 cut(s) 466, 659, 818
BstAPI GCANNNNNTGC 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 329, 365
BstDEI CTNAG 4 cut(s) 102, 717, 855, 897
BstF5I GGATG 3 cut(s) 11, 210, 520
BstH2I RGCGCY 2 cut(s) 334, 492
BstHHI GCGC 3 cut(s) 333, 440, 491
BstKTI GATC 9 cut(s) 34, 91, 185, 196, 731, 826, 868, 976, 1030
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
BstMWI GCNNNNNNNGC 8 cut(s) 52, 206, 220, 315, 324, 408, 417, 550
BstNI CCWGG 3 cut(s) 180, 435, 627
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 5 cut(s) 178, 200, 433, 584, 625
BstSFI CTRYAG 2 cut(s) 144, 484
BstV1I GCAGC 7 cut(s) 201, 232, 368, 429, 452, 470, 498
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 185
BsuRI GGCC 3 cut(s) 209, 510, 682
BsuTUI ATCGAT 1 cut(s) 185
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 3 cut(s) 11, 210, 520
BtsIMutI CAGTG 2 cut(s) 233, 932
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 329, 365
CfoI GCGC 3 cut(s) 333, 440, 491
Cfr10I RCCGGY 2 cut(s) 367, 666
Cfr13I GGNCC 1 cut(s) 664
ClaI ATCGAT 1 cut(s) 185
CpoI CGGWCCG 1 cut(s) 664
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 7 cut(s) 462, 655, 714, 789, 805, 996, 1005
CspAI ACCGGT 1 cut(s) 666
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CspI CGGWCCG 1 cut(s) 664
CviAII CATG 3 cut(s) 592, 697, 797
CviQI GTAC 7 cut(s) 462, 655, 714, 789, 805, 996, 1005
DdeI CTNAG 4 cut(s) 102, 717, 855, 897
DpnI GATC 9 cut(s) 33, 90, 184, 195, 730, 825, 867, 975, 1029
DpnII GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
EaeI YGGCCR 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 964
Eco24I GRGCYC 2 cut(s) 170, 202
Eco32I GATATC 1 cut(s) 852
Eco47I GGWCC 1 cut(s) 664
Eco47III AGCGCT 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 108
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 3 cut(s) 178, 433, 625
EcoRV GATATC 1 cut(s) 852
EcoT14I CCWWGG 1 cut(s) 964
EcoT38I GRGCYC 2 cut(s) 170, 202
ErhI CCWWGG 1 cut(s) 964
FaeI CATG 3 cut(s) 595, 700, 800
FaiI YATR 6 cut(s) 506, 579, 593, 698, 707, 798
FaqI GGGAC 2 cut(s) 602, 789
FatI CATG 3 cut(s) 591, 696, 796
FauI CCCGC 1 cut(s) 868
FbaI TGATCA 2 cut(s) 88, 973
Fnu4HI GCNGC 9 cut(s) 154, 215, 218, 221, 382, 418, 441, 484, 487
FokI GGATG 2 cut(s) 217, 507
FriOI GRGCYC 2 cut(s) 170, 202
Fsp4HI GCNGC 9 cut(s) 154, 215, 218, 221, 382, 418, 441, 484, 487
FspBI CTAG 5 cut(s) 132, 453, 492, 596, 786
GlaI GCGC 3 cut(s) 332, 439, 490
GluI GCNGC 9 cut(s) 154, 215, 218, 221, 382, 418, 441, 484, 487
GsaI CCCAGC 1 cut(s) 304
HaeII RGCGCY 2 cut(s) 334, 492
HaeIII GGCC 3 cut(s) 209, 510, 682
HapII CCGG 5 cut(s) 202, 368, 385, 585, 667
HhaI GCGC 3 cut(s) 333, 440, 491
Hin1II CATG 3 cut(s) 595, 700, 800
Hin6I GCGC 3 cut(s) 331, 438, 489
HinP1I GCGC 3 cut(s) 331, 438, 489
HinfI GANTC 2 cut(s) 254, 925
HpaII CCGG 5 cut(s) 202, 368, 385, 585, 667
HphI GGTGA 3 cut(s) 423, 641, 968
Hpy188I TCNGA 4 cut(s) 93, 127, 664, 900
Hpy188III TCNNGA 3 cut(s) 29, 572, 847
HpyAV CCTTC 1 cut(s) 129
HpyCH4III ACNGT 3 cut(s) 466, 659, 818
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 5 cut(s) 138, 318, 327, 486, 677
HpyF10VI GCNNNNNNNGC 8 cut(s) 52, 206, 220, 315, 324, 408, 417, 550
HpyF3I CTNAG 4 cut(s) 102, 717, 855, 897
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 3 cut(s) 595, 700, 800
HspAI GCGC 3 cut(s) 331, 438, 489
Ksp22I TGATCA 2 cut(s) 88, 973
Kzo9I GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
LmnI GCTCC 2 cut(s) 350, 849
Lsp1109I GCAGC 7 cut(s) 201, 232, 368, 429, 452, 470, 498
LweI GCATC 1 cut(s) 125
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 5 cut(s) 132, 453, 492, 596, 786
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 878, 886
MalI GATC 9 cut(s) 33, 90, 184, 195, 730, 825, 867, 975, 1029
MboI GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
MboII GAAGA 5 cut(s) 114, 465, 560, 1041, 1044
MfeI CAATTG 1 cut(s) 539
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 170, 202
MlsI TGGCCA 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 209
MmeI TCCRAC 3 cut(s) 501, 667, 843
MnlI CCTC 9 cut(s) 71, 111, 255, 315, 338, 341, 368, 371, 565
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
MseI TTAA 4 cut(s) 174, 468, 618, 768
MslI CAYNNNNRTG 2 cut(s) 238, 831
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 2 cut(s) 443, 483
MspI CCGG 5 cut(s) 202, 368, 385, 585, 667
MspR9I CCNGG 5 cut(s) 180, 202, 435, 586, 627
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 1021
MvaI CCWGG 3 cut(s) 180, 435, 627
MwoI GCNNNNNNNGC 8 cut(s) 52, 206, 220, 315, 324, 408, 417, 550
NciI CCSGG 2 cut(s) 202, 586
NdeII GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
NlaIII CATG 3 cut(s) 595, 700, 800
NlaIV GGNNCC 4 cut(s) 33, 117, 352, 583
NspI RCATGY 1 cut(s) 595
PctI GAATGC 1 cut(s) 1021
PfeI GAWTC 2 cut(s) 254, 925
PinAI ACCGGT 1 cut(s) 666
PkrI GCNGC 9 cut(s) 155, 216, 219, 222, 383, 419, 442, 485, 488
PshBI ATTAAT 1 cut(s) 618
Psp6I CCWGG 3 cut(s) 178, 433, 625
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 3 cut(s) 178, 433, 625
PspN4I GGNNCC 4 cut(s) 33, 117, 352, 583
PspPI GGNCC 1 cut(s) 664
PstI CTGCAG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 443, 483
RsaI GTAC 7 cut(s) 463, 656, 715, 790, 806, 997, 1006
RsaNI GTAC 7 cut(s) 462, 655, 714, 789, 805, 996, 1005
RseI CAYNNNNRTG 2 cut(s) 238, 831
Rsr2I CGGWCCG 1 cut(s) 664
RsrII CGGWCCG 1 cut(s) 664
SaqAI TTAA 4 cut(s) 174, 468, 618, 768
SatI GCNGC 9 cut(s) 154, 215, 218, 221, 382, 418, 441, 484, 487
Sau3AI GATC 9 cut(s) 31, 88, 182, 193, 728, 823, 865, 973, 1027
Sau96I GGNCC 1 cut(s) 664
ScaI AGTACT 3 cut(s) 715, 790, 997
ScrFI CCNGG 5 cut(s) 180, 202, 435, 586, 627
SduI GDGCHC 2 cut(s) 170, 202
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 1 cut(s) 125
SfcI CTRYAG 2 cut(s) 144, 484
SinI GGWCC 1 cut(s) 664
SmiMI CAYNNNNRTG 2 cut(s) 238, 831
SmlI CTYRAG 2 cut(s) 355, 457
SmoI CTYRAG 2 cut(s) 355, 457
SpeI ACTAGT 1 cut(s) 785
SsiI CCGC 4 cut(s) 153, 156, 218, 875
SspI AATATT 1 cut(s) 688
SspMI CTAG 5 cut(s) 132, 453, 492, 596, 786
StyD4I CCNGG 5 cut(s) 178, 200, 433, 584, 625
StyI CCWWGG 1 cut(s) 964
TaaI ACNGT 3 cut(s) 466, 659, 818
TaiI ACGT 1 cut(s) 326
TaqI TCGA 4 cut(s) 170, 185, 196, 247
TatI WGTACW 3 cut(s) 713, 788, 995
TauI GCSGC 2 cut(s) 156, 220
TfiI GAWTC 2 cut(s) 254, 925
Tru1I TTAA 4 cut(s) 174, 468, 618, 768
Tru9I TTAA 4 cut(s) 174, 468, 618, 768
TscAI CASTG 2 cut(s) 240, 939
TseI GCWGC 7 cut(s) 214, 220, 381, 417, 440, 483, 486
TspDTI ATGAA 1 cut(s) 713
TspGWI ACGGA 2 cut(s) 4, 897
TspRI CASTG 2 cut(s) 240, 939
VpaK11BI GGWCC 1 cut(s) 664
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 3 cut(s) 638, 937, 949
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 815
XspI CTAG 5 cut(s) 132, 453, 492, 596, 786
ZrmI AGTACT 3 cut(s) 715, 790, 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.