pycom01g15240

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007025
Physical Location & Seq
Forward (+)
15176092 .. 15177358
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15240.1

Sequence Viewer

Length: 1092 bp
ATGGTTTTCTCATCCGTTCCCGTCTATCTGGATCCACCCAACTGGCAACAACAGCAAACAAATCATCATCAACAAGGAGGTGGATGTGATCAGAATAATCCTCAGCTTCCGCAGCAGCCGTCTTCAGAACCTAGGTGTACTTCAAGAGATGGCAGCGGCAGTTTGGGCTCGATCAAACCTGGGTCCATGTCTGATCGAGCCCGGCTGGCCAAGATACCACAGCCAGAGACGGCACTGAAATGCCCTCGATGTGAATCCACAAACACAAAGTTTTGCTACTTCAACAATTACAGCCTTTCCCAGCCTCGCCACTTTTGCAAAACGTGCCGGCGCTACTGGACCAGAGGAGGAGCCTTGAGAAGCGTGCCGGTGGGAGGAGGCTGCCGGCGAAACAAAAGAAGCAAAGGGGGCAACAGCAGCAGGTCAAAATCACCAGGCGCAGCTGGTAATTCAAGCTCAAGTACGGTCAATTCTTCCAACAGCTGCAGCACTAGTGATAATATCATAGGCCGTTTGACACATCCACCACCACCACCACCACCACATCAATTGCCCTTTTTGCCCTCCTTACACCATCTTACTGATTATGGTTCCGGGGACATGATAGGGTTGAATAATTTTGGGGGTATTAATCAACCCGGTGATGTTGAATTCCAACACGATCAGTATGGTTCGGATCGGTTGAGTGCCAGACCAAATATTTTAGGCATGAATAATATGGGTATTTCTGAGCATTGGATCGATCATCACTACTTCAGCAACAATTTCCTAATTTCTTGGCTAATTTGGAACCACCACCTAGTACTACTCATGGGATGTACCAATTTGACGGTGGAGATCGATCAAAATGTTGGCCAGCTTCCGATATCTAAGCCTTTAGATCATTCGGTGGGTAACGGAGTTACTACTACTCAGATGGTTAATGTGAAAATGGAACACAGTCATCAAGCACTGAATTTGTCAAGGAATTTTTTGGGAAGTCTGGGAAATGATCACCAATACTGGGGTATTAGTACTGCTGGTACTGGTGGCAATGCTTGGACCGATCTTTCTGGTTTCACATCTTCTTCTTCTACGACCCATCTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

364

Amino Acids

39.67

Weight (kDa)

8.81

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 78 - 133 2.1e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 411
AciI CCGC 2 cut(s) 110, 156
AclWI GGATC 4 cut(s) 26, 39, 684, 746
AcoI YGGCCR 2 cut(s) 207, 853
AcsI RAATTY 3 cut(s) 650, 955, 967
AcuI CTGAAG 2 cut(s) 108, 739
AfaI GTAC 6 cut(s) 139, 463, 804, 820, 1015, 1024
AfiI CCNNNNNNNGG 2 cut(s) 374, 1003
AgsI TTSAA 5 cut(s) 144, 283, 453, 613, 650
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 2 cut(s) 178, 433
AluBI AGCT 5 cut(s) 106, 443, 456, 483, 859
AluI AGCT 5 cut(s) 106, 443, 456, 483, 859
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 4 cut(s) 26, 39, 684, 746
AoxI GGCC 3 cut(s) 207, 508, 853
ApeKI GCWGC 8 cut(s) 112, 115, 153, 381, 417, 440, 483, 486
ApoI RAATTY 3 cut(s) 650, 955, 967
AseI ATTAAT 1 cut(s) 630
AspA2I CCTAGG 1 cut(s) 131
AspLEI GCGC 2 cut(s) 333, 440
AspS9I GGNCC 3 cut(s) 183, 339, 1041
AsuC2I CCSGG 3 cut(s) 202, 595, 639
AsuHPI GGTGA 3 cut(s) 423, 653, 986
AvaII GGWCC 3 cut(s) 183, 339, 1041
AvrII CCTAGG 1 cut(s) 131
BalI TGGCCA 2 cut(s) 209, 855
BamHI GGATCC 1 cut(s) 31
BanII GRGCYC 2 cut(s) 170, 202
BbsI GAAGAC 1 cut(s) 114
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 8 cut(s) 124, 127, 165, 368, 429, 452, 470, 498
BccI CCATC 4 cut(s) 143, 582, 910, 1089
BceAI ACGGC 3 cut(s) 103, 246, 495
BciT130I CCWGG 2 cut(s) 180, 435
BclI TGATCA 2 cut(s) 88, 991
BcnI CCSGG 3 cut(s) 202, 595, 639
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 3 cut(s) 132, 492, 800
BfmI CTRYAG 1 cut(s) 484
BfoI RGCGCY 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 411
BglI GCCNNNNNGGC 1 cut(s) 206
BisI GCNGC 9 cut(s) 113, 116, 154, 157, 382, 418, 441, 484, 487
BlnI CCTAGG 1 cut(s) 131
BlsI GCNGC 9 cut(s) 114, 117, 155, 158, 383, 419, 442, 485, 488
BmcAI AGTACT 2 cut(s) 804, 1015
Bme1390I CCNGG 5 cut(s) 180, 202, 435, 595, 639
Bme18I GGWCC 3 cut(s) 183, 339, 1041
BmgT120I GGNCC 3 cut(s) 183, 339, 1041
BmiI GGNNCC 5 cut(s) 33, 184, 352, 592, 791
BmrFI CCNGG 5 cut(s) 180, 202, 435, 595, 639
BmrI ACTGGG 1 cut(s) 1012
BmuI ACTGGG 1 cut(s) 1012
BpiI GAAGAC 1 cut(s) 114
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 376, 442
BpuMI CCSGG 3 cut(s) 202, 595, 639
Bsa29I ATCGAT 2 cut(s) 741, 840
BsaBI GATNNNNATC 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 131, 179, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 1003
Bse118I RCCGGY 3 cut(s) 327, 367, 384
Bse1I ACTGG 4 cut(s) 47, 341, 1007, 1030
Bse3DI GCAATG 1 cut(s) 1039
Bse8I GATNNNNATC 1 cut(s) 253
BseBI CCWGG 2 cut(s) 180, 435
BseCI ATCGAT 2 cut(s) 741, 840
BseDI CCNNGG 3 cut(s) 131, 179, 594
BseGI GGATG 4 cut(s) 11, 89, 520, 821
BseJI GATNNNNATC 1 cut(s) 253
BseLI CCNNNNNNNGG 2 cut(s) 374, 1003
BseMI GCAATG 1 cut(s) 1039
BseMII CTCAG 3 cut(s) 116, 720, 926
BseNI ACTGG 4 cut(s) 47, 341, 1007, 1030
BseRI GAGGAG 3 cut(s) 360, 363, 390
BseXI GCAGC 8 cut(s) 124, 127, 165, 368, 429, 452, 470, 498
BseYI CCCAGC 1 cut(s) 300
BshFI GGCC 3 cut(s) 209, 510, 855
BshVI ATCGAT 2 cut(s) 741, 840
BsiSI CCGG 6 cut(s) 202, 328, 368, 385, 594, 639
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 2 cut(s) 374, 1003
BsmAI GTCTC 1 cut(s) 221
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 3 cut(s) 209, 510, 855
Bsp1286I GDGCHC 2 cut(s) 170, 202
BspACI CCGC 2 cut(s) 110, 156
BspANI GGCC 3 cut(s) 209, 510, 855
BspCNI CTCAG 3 cut(s) 115, 721, 925
BspDI ATCGAT 2 cut(s) 741, 840
BspLI GGNNCC 5 cut(s) 33, 184, 352, 592, 791
BspMAI CTGCAG 1 cut(s) 488
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 4 cut(s) 26, 39, 684, 746
BsrDI GCAATG 1 cut(s) 1039
BsrFI RCCGGY 3 cut(s) 327, 367, 384
BsrI ACTGG 4 cut(s) 47, 341, 1007, 1030
BssAI RCCGGY 3 cut(s) 327, 367, 384
BssECI CCNNGG 3 cut(s) 131, 179, 594
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 2 cut(s) 180, 435
Bst4CI ACNGT 3 cut(s) 466, 832, 941
BstAPI GCANNNNNTGC 1 cut(s) 324
BstC8I GCNNGC 5 cut(s) 207, 329, 365, 386, 857
BstDEI CTNAG 4 cut(s) 102, 729, 870, 912
BstF5I GGATG 4 cut(s) 11, 89, 520, 821
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 2 cut(s) 333, 440
BstMAI GTCTC 1 cut(s) 221
BstMWI GCNNNNNNNGC 9 cut(s) 52, 112, 165, 206, 315, 324, 408, 417, 559
BstNI CCWGG 2 cut(s) 180, 435
BstSCI CCNGG 5 cut(s) 178, 200, 433, 593, 637
BstSFI CTRYAG 1 cut(s) 484
BstV1I GCAGC 8 cut(s) 124, 127, 165, 368, 429, 452, 470, 498
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 2 cut(s) 741, 840
BsuRI GGCC 3 cut(s) 209, 510, 855
BsuTUI ATCGAT 2 cut(s) 741, 840
BtsCI GGATG 4 cut(s) 11, 89, 520, 821
BtsIMutI CAGTG 2 cut(s) 233, 950
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 5 cut(s) 207, 329, 365, 386, 857
CfoI GCGC 2 cut(s) 333, 440
Cfr10I RCCGGY 3 cut(s) 327, 367, 384
Cfr13I GGNCC 3 cut(s) 183, 339, 1041
ClaI ATCGAT 2 cut(s) 741, 840
Csp6I GTAC 6 cut(s) 138, 462, 803, 819, 1014, 1023
CspCI CAANNNNNGTGG 2 cut(s) 531, 566
CviAII CATG 4 cut(s) 187, 601, 709, 811
CviQI GTAC 6 cut(s) 138, 462, 803, 819, 1014, 1023
DdeI CTNAG 4 cut(s) 102, 729, 870, 912
EaeI YGGCCR 2 cut(s) 207, 853
Eco130I CCWWGG 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 170, 202
Eco32I GATATC 1 cut(s) 867
Eco47I GGWCC 3 cut(s) 183, 339, 1041
Eco57I CTGAAG 2 cut(s) 108, 739
EcoRI GAATTC 1 cut(s) 650
EcoRII CCWGG 2 cut(s) 178, 433
EcoRV GATATC 1 cut(s) 867
EcoT14I CCWWGG 1 cut(s) 131
EcoT38I GRGCYC 2 cut(s) 170, 202
ErhI CCWWGG 1 cut(s) 131
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 4 cut(s) 190, 604, 712, 814
FaiI YATR 8 cut(s) 188, 506, 588, 602, 669, 710, 719, 812
FaqI GGGAC 1 cut(s) 611
FatI CATG 4 cut(s) 186, 600, 708, 810
FbaI TGATCA 2 cut(s) 88, 991
Fnu4HI GCNGC 9 cut(s) 113, 116, 154, 157, 382, 418, 441, 484, 487
FokI GGATG 3 cut(s) 96, 507, 828
FriOI GRGCYC 2 cut(s) 170, 202
Fsp4HI GCNGC 9 cut(s) 113, 116, 154, 157, 382, 418, 441, 484, 487
FspBI CTAG 3 cut(s) 132, 492, 800
GlaI GCGC 2 cut(s) 332, 439
GluI GCNGC 9 cut(s) 113, 116, 154, 157, 382, 418, 441, 484, 487
GsaI CCCAGC 1 cut(s) 304
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 3 cut(s) 209, 510, 855
HapII CCGG 6 cut(s) 202, 328, 368, 385, 594, 639
HhaI GCGC 2 cut(s) 333, 440
Hin1II CATG 4 cut(s) 190, 604, 712, 814
Hin6I GCGC 2 cut(s) 331, 438
HinP1I GCGC 2 cut(s) 331, 438
HinfI GANTC 1 cut(s) 254
HpaII CCGG 6 cut(s) 202, 328, 368, 385, 594, 639
HphI GGTGA 3 cut(s) 423, 653, 986
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 7 cut(s) 93, 127, 193, 676, 730, 864, 915
Hpy188III TCNNGA 2 cut(s) 29, 144
Hpy8I GTNNAC 1 cut(s) 138
HpyCH4III ACNGT 3 cut(s) 466, 832, 941
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 2 cut(s) 318, 486
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 112, 165, 206, 315, 324, 408, 417, 559
HpyF3I CTNAG 4 cut(s) 102, 729, 870, 912
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 4 cut(s) 190, 604, 712, 814
HspAI GCGC 2 cut(s) 331, 438
KroI GCCGGC 2 cut(s) 327, 384
KroNI GCCGGC 2 cut(s) 329, 386
Ksp22I TGATCA 2 cut(s) 88, 991
LmnI GCTCC 1 cut(s) 350
Lsp1109I GCAGC 8 cut(s) 124, 127, 165, 368, 429, 452, 470, 498
MaeI CTAG 3 cut(s) 132, 492, 800
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 893, 901
MboII GAAGA 5 cut(s) 114, 465, 1056, 1059, 1062
MfeI CAATTG 1 cut(s) 548
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 170, 202
MlsI TGGCCA 2 cut(s) 209, 855
MluNI TGGCCA 2 cut(s) 209, 855
MmeI TCCRAC 2 cut(s) 501, 679
MnlI CCTC 9 cut(s) 71, 111, 255, 315, 338, 341, 368, 371, 574
Mox20I TGGCCA 2 cut(s) 209, 855
MroNI GCCGGC 2 cut(s) 327, 384
MscI TGGCCA 2 cut(s) 209, 855
MseI TTAA 2 cut(s) 630, 921
MslI CAYNNNNRTG 1 cut(s) 238
Msp20I TGGCCA 2 cut(s) 209, 855
MspA1I CMGCKG 3 cut(s) 156, 443, 483
MspI CCGG 6 cut(s) 202, 328, 368, 385, 594, 639
MspR9I CCNGG 5 cut(s) 180, 202, 435, 595, 639
MunI CAATTG 1 cut(s) 548
MvaI CCWGG 2 cut(s) 180, 435
MwoI GCNNNNNNNGC 9 cut(s) 52, 112, 165, 206, 315, 324, 408, 417, 559
NaeI GCCGGC 2 cut(s) 329, 386
NciI CCSGG 3 cut(s) 202, 595, 639
NgoMIV GCCGGC 2 cut(s) 327, 384
NlaIII CATG 4 cut(s) 190, 604, 712, 814
NlaIV GGNNCC 5 cut(s) 33, 184, 352, 592, 791
PdiI GCCGGC 2 cut(s) 329, 386
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 9 cut(s) 114, 117, 155, 158, 383, 419, 442, 485, 488
PshBI ATTAAT 1 cut(s) 630
Psp6I CCWGG 2 cut(s) 178, 433
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 2 cut(s) 178, 433
PspN4I GGNNCC 5 cut(s) 33, 184, 352, 592, 791
PspPI GGNCC 3 cut(s) 183, 339, 1041
PstI CTGCAG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 443, 483
RsaI GTAC 6 cut(s) 139, 463, 804, 820, 1015, 1024
RsaNI GTAC 6 cut(s) 138, 462, 803, 819, 1014, 1023
RseI CAYNNNNRTG 1 cut(s) 238
SaqAI TTAA 2 cut(s) 630, 921
SatI GCNGC 9 cut(s) 113, 116, 154, 157, 382, 418, 441, 484, 487
Sau96I GGNCC 3 cut(s) 183, 339, 1041
ScaI AGTACT 2 cut(s) 804, 1015
ScrFI CCNGG 5 cut(s) 180, 202, 435, 595, 639
SduI GDGCHC 2 cut(s) 170, 202
SfcI CTRYAG 1 cut(s) 484
SinI GGWCC 3 cut(s) 183, 339, 1041
SmiMI CAYNNNNRTG 1 cut(s) 238
SmlI CTYRAG 2 cut(s) 355, 457
SmoI CTYRAG 2 cut(s) 355, 457
SpeI ACTAGT 1 cut(s) 491
SsiI CCGC 2 cut(s) 110, 156
SspI AATATT 1 cut(s) 700
SspMI CTAG 3 cut(s) 132, 492, 800
StyD4I CCNGG 5 cut(s) 178, 200, 433, 593, 637
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 3 cut(s) 466, 832, 941
TaiI ACGT 1 cut(s) 326
TaqI TCGA 5 cut(s) 170, 196, 247, 741, 840
TaqII GACCGA 1 cut(s) 1058
TatI WGTACW 3 cut(s) 137, 802, 1013
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 2 cut(s) 630, 921
Tru9I TTAA 2 cut(s) 630, 921
TscAI CASTG 2 cut(s) 240, 957
TseI GCWGC 8 cut(s) 112, 115, 153, 381, 417, 440, 483, 486
TspDTI ATGAA 1 cut(s) 725
TspGWI ACGGA 2 cut(s) 4, 912
TspRI CASTG 2 cut(s) 240, 957
VpaK11BI GGWCC 3 cut(s) 183, 339, 1041
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 3 cut(s) 650, 955, 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XmaJI CCTAGG 1 cut(s) 131
XspI CTAG 3 cut(s) 132, 492, 800
ZrmI AGTACT 2 cut(s) 804, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.