pycom07g18480

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007025
Physical Location & Seq
Forward (+)
20924223 .. 20925160
938 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18480.1

Sequence Viewer

Length: 759 bp
ATGGTTTTCTCATCCGTTCCCGTCTATCTGGATCCACCCAACTGGCAACAACAGCAAACAAATCATCATCAACAAGGAGGTGGAAGTGATCAGAATAATCCTCAGCTTCCATTGGCGCCTTCTTCAGAAGCTAGATGCACTTCTGTAGATGGCGGCGGTGGTTTGGGCTCGATTAAACCTGGATCGATGTGTGATCGAGCGCGGATGGCCAACATGCCGCAGCCAGAGACAGCACTGAAATGCCCTCGATGTGAATCCACAAACACAAAGTTTTGCTACTTCAACAATTACAACCTTTCCCAGCCTCGCCACTTTTGCAAGACGTGCAGGCGCTACTGGACAAGAGGAGGAGCCTTGAGAAGCGTGCCGGTGGGAGGAGGCTGCCGGAGAAATAAAAGAAGCAAAGGAGGCAACAGCAGCAGGTCAAAATCACCAGGCGCAGCTGGTAATTCTAGCTCAAGTACGGTTAATTCTTCCAACAGCTGCAGCGCTAGTGATAATATCATAGGCCATTTGACACATCCACCACCACCACATCAATTGCCGTTTTTGCCCTCCTTACACCATCTTCCTGATTATGGTTCCGGGGACATGCTAGGGCTAAATAATTTTGGGATTAATCAACCTGGTGATGTTGAATTCCAACACCATCAGTACGGTTCGGACCGGTTCAGTGCAAGGCCAAATATTTTAGGCATGAATAATATGAGTAATACTGAGCATTGGAGATCGATCACTACTTCAACAGCAATTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

253

Amino Acids

27.34

Weight (kDa)

9.28

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 78 - 133 1.2e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 115
AccII CGCG 1 cut(s) 202
AciI CCGC 4 cut(s) 153, 156, 202, 218
AclWI GGATC 3 cut(s) 26, 39, 190
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 638
AcuI CTGAAG 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 2 cut(s) 463, 656
AfeI AGCGCT 1 cut(s) 490
AfiI CCNNNNNNNGG 2 cut(s) 374, 578
AgeI ACCGGT 1 cut(s) 666
AgsI TTSAA 3 cut(s) 283, 638, 744
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 3 cut(s) 178, 433, 625
AluBI AGCT 5 cut(s) 106, 131, 443, 456, 483
AluI AGCT 5 cut(s) 106, 131, 443, 456, 483
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 3 cut(s) 26, 39, 190
Aor51HI AGCGCT 1 cut(s) 490
AoxI GGCC 3 cut(s) 207, 508, 680
ApeKI GCWGC 6 cut(s) 220, 381, 417, 440, 483, 486
ApoI RAATTY 1 cut(s) 638
AseI ATTAAT 1 cut(s) 618
AsiGI ACCGGT 1 cut(s) 666
AspLEI GCGC 5 cut(s) 118, 202, 333, 440, 491
AspS9I GGNCC 1 cut(s) 664
AsuC2I CCSGG 1 cut(s) 586
AsuHPI GGTGA 2 cut(s) 423, 641
AvaII GGWCC 1 cut(s) 664
BalI TGGCCA 1 cut(s) 209
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 170
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 6 cut(s) 232, 368, 429, 452, 470, 498
BccI CCATC 4 cut(s) 143, 199, 573, 657
BceAI ACGGC 1 cut(s) 529
BciT130I CCWGG 3 cut(s) 180, 435, 627
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 4 cut(s) 132, 453, 492, 596
BfmI CTRYAG 2 cut(s) 144, 484
BfoI RGCGCY 3 cut(s) 119, 334, 492
BfuAI ACCTGC 1 cut(s) 411
BisI GCNGC 8 cut(s) 154, 218, 221, 382, 418, 441, 484, 487
BlsI GCNGC 8 cut(s) 155, 219, 222, 383, 419, 442, 485, 488
Bme1390I CCNGG 4 cut(s) 180, 435, 586, 627
Bme18I GGWCC 1 cut(s) 664
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 664
BmiI GGNNCC 4 cut(s) 33, 117, 352, 583
BmrFI CCNGG 4 cut(s) 180, 435, 586, 627
BmsI GCATC 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 376, 442
BpuMI CCSGG 1 cut(s) 586
Bsa29I ATCGAT 2 cut(s) 185, 731
BsaBI GATNNNNATC 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 585
BsaWI WCCGGW 1 cut(s) 666
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 578
Bse118I RCCGGY 2 cut(s) 367, 666
Bse1I ACTGG 2 cut(s) 47, 341
Bse8I GATNNNNATC 1 cut(s) 253
BseBI CCWGG 3 cut(s) 180, 435, 627
BseCI ATCGAT 2 cut(s) 185, 731
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 3 cut(s) 11, 210, 520
BseJI GATNNNNATC 1 cut(s) 253
BseLI CCNNNNNNNGG 2 cut(s) 374, 578
BseMII CTCAG 2 cut(s) 116, 708
BseNI ACTGG 2 cut(s) 47, 341
BseRI GAGGAG 3 cut(s) 360, 363, 390
BseXI GCAGC 6 cut(s) 232, 368, 429, 452, 470, 498
BseYI CCCAGC 1 cut(s) 300
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 1 cut(s) 202
BshFI GGCC 3 cut(s) 209, 510, 682
BshNI GGYRCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 666
BshVI ATCGAT 2 cut(s) 185, 731
BsiSI CCGG 4 cut(s) 368, 385, 585, 667
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 374, 578
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 3 cut(s) 209, 510, 682
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 6 cut(s) 31, 88, 182, 193, 728, 732
BspACI CCGC 4 cut(s) 153, 156, 202, 218
BspANI GGCC 3 cut(s) 209, 510, 682
BspCNI CTCAG 2 cut(s) 115, 709
BspDI ATCGAT 2 cut(s) 185, 731
BspFNI CGCG 1 cut(s) 202
BspLI GGNNCC 4 cut(s) 33, 117, 352, 583
BspMAI CTGCAG 1 cut(s) 488
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 3 cut(s) 26, 39, 190
BspT107I GGYRCC 1 cut(s) 115
BsrFI RCCGGY 2 cut(s) 367, 666
BsrI ACTGG 2 cut(s) 47, 341
BssAI RCCGGY 2 cut(s) 367, 666
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 6 cut(s) 31, 88, 182, 193, 728, 732
BssNI GRCGYC 1 cut(s) 116
Bst2UI CCWGG 3 cut(s) 180, 435, 627
Bst4CI ACNGT 2 cut(s) 466, 659
BstACI GRCGYC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 329, 365
BstDEI CTNAG 2 cut(s) 102, 717
BstF5I GGATG 3 cut(s) 11, 210, 520
BstFNI CGCG 1 cut(s) 202
BstH2I RGCGCY 3 cut(s) 119, 334, 492
BstHHI GCGC 5 cut(s) 118, 202, 333, 440, 491
BstKTI GATC 6 cut(s) 34, 91, 185, 196, 731, 735
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 6 cut(s) 31, 88, 182, 193, 728, 732
BstMWI GCNNNNNNNGC 7 cut(s) 52, 206, 315, 324, 408, 417, 550
BstNI CCWGG 3 cut(s) 180, 435, 627
BstNSI RCATGY 2 cut(s) 217, 595
BstSCI CCNGG 4 cut(s) 178, 433, 584, 625
BstSFI CTRYAG 2 cut(s) 144, 484
BstUI CGCG 1 cut(s) 202
BstV1I GCAGC 6 cut(s) 232, 368, 429, 452, 470, 498
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 2 cut(s) 185, 731
BsuRI GGCC 3 cut(s) 209, 510, 682
BsuTUI ATCGAT 2 cut(s) 185, 731
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 3 cut(s) 11, 210, 520
BtsIMutI CAGTG 2 cut(s) 233, 679
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 329, 365
CfoI GCGC 5 cut(s) 118, 202, 333, 440, 491
Cfr10I RCCGGY 2 cut(s) 367, 666
Cfr13I GGNCC 1 cut(s) 664
ClaI ATCGAT 2 cut(s) 185, 731
CpoI CGGWCCG 1 cut(s) 664
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 2 cut(s) 462, 655
CspAI ACCGGT 1 cut(s) 666
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CspI CGGWCCG 1 cut(s) 664
CviAII CATG 3 cut(s) 214, 592, 697
CviQI GTAC 2 cut(s) 462, 655
DdeI CTNAG 2 cut(s) 102, 717
DinI GGCGCC 1 cut(s) 117
DpnI GATC 6 cut(s) 33, 90, 184, 195, 730, 734
DpnII GATC 6 cut(s) 31, 88, 182, 193, 728, 732
EaeI YGGCCR 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 664
Eco47III AGCGCT 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 108
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 3 cut(s) 178, 433, 625
EcoT38I GRGCYC 1 cut(s) 170
EgeI GGCGCC 1 cut(s) 117
EheI GGCGCC 1 cut(s) 117
FaeI CATG 3 cut(s) 217, 595, 700
FaiI YATR 6 cut(s) 215, 506, 579, 593, 698, 707
FaqI GGGAC 1 cut(s) 602
FatI CATG 3 cut(s) 213, 591, 696
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 8 cut(s) 154, 218, 221, 382, 418, 441, 484, 487
FokI GGATG 2 cut(s) 217, 507
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 8 cut(s) 154, 218, 221, 382, 418, 441, 484, 487
FspBI CTAG 4 cut(s) 132, 453, 492, 596
GlaI GCGC 5 cut(s) 117, 201, 332, 439, 490
GluI GCNGC 8 cut(s) 154, 218, 221, 382, 418, 441, 484, 487
GsaI CCCAGC 1 cut(s) 304
HaeII RGCGCY 3 cut(s) 119, 334, 492
HaeIII GGCC 3 cut(s) 209, 510, 682
HapII CCGG 4 cut(s) 368, 385, 585, 667
HhaI GCGC 5 cut(s) 118, 202, 333, 440, 491
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 3 cut(s) 217, 595, 700
Hin6I GCGC 5 cut(s) 116, 200, 331, 438, 489
HinP1I GCGC 5 cut(s) 116, 200, 331, 438, 489
HinfI GANTC 1 cut(s) 254
HpaII CCGG 4 cut(s) 368, 385, 585, 667
HphI GGTGA 2 cut(s) 423, 641
Hpy188I TCNGA 3 cut(s) 93, 127, 664
Hpy188III TCNNGA 2 cut(s) 29, 572
HpyAV CCTTC 1 cut(s) 129
HpyCH4III ACNGT 2 cut(s) 466, 659
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 5 cut(s) 138, 318, 327, 486, 677
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 206, 315, 324, 408, 417, 550
HpyF3I CTNAG 2 cut(s) 102, 717
HpySE526I ACGT 1 cut(s) 323
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 3 cut(s) 217, 595, 700
HspAI GCGC 5 cut(s) 116, 200, 331, 438, 489
KasI GGCGCC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 6 cut(s) 31, 88, 182, 193, 728, 732
LmnI GCTCC 1 cut(s) 350
Lsp1109I GCAGC 6 cut(s) 232, 368, 429, 452, 470, 498
LweI GCATC 1 cut(s) 125
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 4 cut(s) 132, 453, 492, 596
MaeII ACGT 1 cut(s) 323
MalI GATC 6 cut(s) 33, 90, 184, 195, 730, 734
MboI GATC 6 cut(s) 31, 88, 182, 193, 728, 732
MboII GAAGA 3 cut(s) 114, 465, 560
MfeI CAATTG 1 cut(s) 539
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 170
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 7 cut(s) 286, 448, 469, 539, 607, 638, 750
MluNI TGGCCA 1 cut(s) 209
Mly113I GGCGCC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 501, 667
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
MseI TTAA 3 cut(s) 174, 468, 618
MslI CAYNNNNRTG 1 cut(s) 238
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 2 cut(s) 443, 483
MspI CCGG 4 cut(s) 368, 385, 585, 667
MspR9I CCNGG 4 cut(s) 180, 435, 586, 627
MunI CAATTG 1 cut(s) 539
MvaI CCWGG 3 cut(s) 180, 435, 627
MvnI CGCG 1 cut(s) 202
MwoI GCNNNNNNNGC 7 cut(s) 52, 206, 315, 324, 408, 417, 550
NarI GGCGCC 1 cut(s) 116
NciI CCSGG 1 cut(s) 586
NdeII GATC 6 cut(s) 31, 88, 182, 193, 728, 732
NlaIII CATG 3 cut(s) 217, 595, 700
NlaIV GGNNCC 4 cut(s) 33, 117, 352, 583
NspI RCATGY 2 cut(s) 217, 595
PfeI GAWTC 1 cut(s) 254
PinAI ACCGGT 1 cut(s) 666
PkrI GCNGC 8 cut(s) 155, 219, 222, 383, 419, 442, 485, 488
PluTI GGCGCC 1 cut(s) 119
PshBI ATTAAT 1 cut(s) 618
Psp6I CCWGG 3 cut(s) 178, 433, 625
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 3 cut(s) 178, 433, 625
PspN4I GGNNCC 4 cut(s) 33, 117, 352, 583
PspPI GGNCC 1 cut(s) 664
PstI CTGCAG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 443, 483
RsaI GTAC 2 cut(s) 463, 656
RsaNI GTAC 2 cut(s) 462, 655
RseI CAYNNNNRTG 1 cut(s) 238
Rsr2I CGGWCCG 1 cut(s) 664
RsrII CGGWCCG 1 cut(s) 664
SaqAI TTAA 3 cut(s) 174, 468, 618
SatI GCNGC 8 cut(s) 154, 218, 221, 382, 418, 441, 484, 487
Sau3AI GATC 6 cut(s) 31, 88, 182, 193, 728, 732
Sau96I GGNCC 1 cut(s) 664
ScrFI CCNGG 4 cut(s) 180, 435, 586, 627
SduI GDGCHC 1 cut(s) 170
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 1 cut(s) 125
SfcI CTRYAG 2 cut(s) 144, 484
SfoI GGCGCC 1 cut(s) 117
SinI GGWCC 1 cut(s) 664
SmiMI CAYNNNNRTG 1 cut(s) 238
SmlI CTYRAG 2 cut(s) 355, 457
SmoI CTYRAG 2 cut(s) 355, 457
Sse9I AATT 7 cut(s) 286, 448, 469, 539, 607, 638, 750
SsiI CCGC 4 cut(s) 153, 156, 202, 218
SspDI GGCGCC 1 cut(s) 115
SspI AATATT 1 cut(s) 688
SspMI CTAG 4 cut(s) 132, 453, 492, 596
StyD4I CCNGG 4 cut(s) 178, 433, 584, 625
TaaI ACNGT 2 cut(s) 466, 659
TaiI ACGT 1 cut(s) 326
TaqI TCGA 5 cut(s) 170, 185, 196, 247, 731
TasI AATT 7 cut(s) 286, 448, 469, 539, 607, 638, 750
TauI GCSGC 2 cut(s) 156, 220
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 3 cut(s) 174, 468, 618
Tru9I TTAA 3 cut(s) 174, 468, 618
TscAI CASTG 2 cut(s) 240, 679
TseI GCWGC 6 cut(s) 220, 381, 417, 440, 483, 486
TspDTI ATGAA 1 cut(s) 713
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 2 cut(s) 240, 679
VpaK11BI GGWCC 1 cut(s) 664
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 1 cut(s) 638
XceI RCATGY 2 cut(s) 217, 595
XspI CTAG 4 cut(s) 132, 453, 492, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.