Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001807

Physical Location & Seq

Genomic Coordinates Reverse (-)

24831168 .. 24836738

Gene Structure 5571 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1138700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4410 bp

ATGGGGCGTCTAAAGCTGCAAACTGGCATCAAGGCAATTGAGGAAGAACCCGAGGACTGTGAGGCAGCCAATTCAAATAAAGCTACGTTGGCATGCATCATCAATTCAGAAATAGGTTCGGTATTAGCGGTTATGAGGAGGAATGTAAGATGGGGCAGTCGTTATATATCCGGTGATGATCAGCTGGAACACTCTTTAATCCAGTCTTTAAAGGCATTGCGCAAGCAAATTTTTTCATGGCAGCATCAGTGGCATACCATTAATCCTGTTGTGTATCTCCAGCCATTTTTGGATGTGATTAGATCTGATGAAACTGGTGCACCAATCACTGGTGCTGCTTTGTCATCTGTTTATAATATCTTAACACTTGATGTGATTGATCAAAATTCCGTAAATGTTGAAGATGCCATGAACTTGTTAGTTGATGCCATCACTAGTTGTCGATTTGAGGTCACCGATCCTGCATCAGAGGAAGTGGTACTGATGAAGATACTACAGGTTCTTCTGGCTTGCATGAAGAGTAAAGCATCTGTTATGTTGAGTAACCAGCATGTTTGCACTATAGTGAATACGTGCTTTCGTATCGTTCATCAAGCAGGAACAAAAGGCGAGTTGTTGCAACGAATAGCTCGCCACACAATGCATGAACTTGTTAGATGTATTTTCTCACACCTTCCTGATGTTCACGACACTGAACGTGCACTGTTAAATGGAAGTACTACAAGAAAACAAGAGATTGCTGGGCTAAATAATGAGTATGCTTTTGGGAGTAGACAATTGGAGAATGGCAATTTGAGTTCTGGGTATGATGGTCAACCATTATCCACAAGTCCTCCCTCAAATGCTTCTTCAGGCCTGGTAGCACCTGGGATTGATGAAAGTGTACTCACGGTTTCTACTGAGAAGGAGGCTGTTCAATATGATTTGCATATCATGACTGAGCCATATGGGGTACCCTGCATGGTGGAAATATTTCACTTTCTGTGCTCTTTGTTAAATGTTTCTGAGCATATGGGGGTGGGACCCAGATCTAGTACCATAACATTTGATGAAGATGTGCCTCTTTTTGCCTTGGTTATGATTAATTCAGCTATAGAGTTGGGTGGATCCCACATTCAGAATCATCCCAAGTTATTGAGTTTAGTTCAAGATGAATTGTTTCGAAATCTTATGCAGTTTGGTTTGTCAACGAGTCCACTTATTCTTTCAATGGTATGCAGCATTGTTCTTAATTTATACCACCATTTGCGCACTGAACTTAAACTACAGCTTGAGGCCTTCTTTTCTTGTGTGATTTTGAGGCTTGCACAAAGTAAATATGGAGCTTCTTATCAGCAGCAGGAGGTTGCTATGGAGGTTCTTGTTGACTTCTGCAGGCAGAAAAAATTCATGGTGGAGATGTACGCTAACTTAGATTGTGACATAACATGCAGCAATGTCTTTGAAGAACTTGCTAATCTGTTGTCAAAGAGTGCATTCCCTGTCAATTGCCCTTTGTCTTCAATTCACATTCTTGCTTTGGATGGCCTTATTGCTGTTATTCAAGGAATGGCCGAAAGGGTGGGGAATGGATCAGTTTCTGCACATACGCCAGTGAATCTTGAAGAGTATACTCCATTCTGGATGGTGAAGTGTGACAACTACAGTGATCCTAGTAATTGGGTTCCATTTGTTCGCCGGAGGAAGTACATAAAGAGAAGGTTAATGATTGGAGCTGATCATTTTAACCGTGACCCAAAGAAAGGACTGGAGTTCCTCCAAGGAACATATCTGTTGCCTGACAAACTTGATCCACAAAGTGTGGCCTGCTTTTTCAGGTACACTGCTGGGTTGGACAAGAATCTAGTTGGGGATTTCTTGGGAAATCATGACGAGTTTTGTGTTCAGGTTCTTCATAAATTTGCCGGTACCTTTGATTTCGCAGATATGAACTTGGATACTGCACTCCGACTGTTTTTGGAGACATTTCGATTGCCTGGAGAATCACAAAAGATACAAAGGGTGCTTGAAGCATTCTCAGAGAGATATTATGAGCAATCACCACTAATTCTAGCTAACAAGGATGCTGCGCTTTTGCTGTCATATTCACTCATAATGCTCAACACAGATCAACACAATGTTCAGGTAAAGAAGAAAATGACGGAGGAGGATTTCATTCGGAATAATCGGCACATCAATGGAGGCAGTGATCTTCCTCGAGAATTTCTGACAGAGCTTTACCACTCAATATGCAAGAATGAGATCCGAACAACTCCAGAACAAGGTGCTGGTTATCCTGAAATGACCCCAAGCCGGTGGATTGATTTAATGCACAAATCTAAGAAGAACGCTCCATTTATCATGTCTGATTCCAGAGCCTACCTAGACCACGATATGTTTGCTATAATGTCGGGCCCTACAATTGCTGCTATCTCTGTGGTATTTGATCATGCAGAACATGAAGAGATTTACCAAACTTGCATAGATGGATTTTTAGCCGTTGCAAAGATATCAGCATGCCATCATCTTGAAGATGTCCTGGATGATCTGGTTGTATCTCTCTGTAAGTTTACCACCCTTTTAAACCCATCATCTGTTGAGGAGCCTGTGCTAGCCTTTGGTGATGACACAAAAGCAAGAATGGCAACTGTGACTGTATTTACAATTGCCAATAGGTATGGTGATTACATTCGCACAGGTTGGAGAAATATTCTGGACTGCATCTTAAGATTACACAAGCTTGGCCTTCTTCCAGCTCGTGTGGCCAGTGATGCAGCCGATGAGTCGGAGTTTTCAGCTGACACAGGGCCTGGGAAGCCTATAACAAATTCTCTCTCTTCTGTTCATGTATCTTCCATAGGTACTCCTAGGAGATCCTCTGGATTGATGGGTCGGTTTAGTCAGCTCTTATCTCTTGAGACAGAGGAGCCAAGATCGCAGCCTACTGAACAACAACTTGCTGCTCATCAACGCACCCTTCAGACAATTCAAAAGTGCCATATTGACAGCATATTTACCGAGAGCAAGTTTCTGCAGGCTGAATCCCTCTTACAGCTTGCACGCGCACTGAATTGGGCTGCAGGACGACCCCAGAAAGGGAATAGCTCTCCTGAGGACGAGGATACTGCAGTTTTCTGTTTGGAGTTGCTGATTGCTATTACCCTAAATAATAGGGATAGGATTGATCTTCTTTGGCAGGGAGTGTATGAGCACATATCCAACATTGTTCAGTCAACTGTGATGCCTTGTGCTCTGGTAGAGAAGGCTGTTTTTGGACTTCTTCGGATTTGCCAGCGGCTGCTTCCCTATAAAGAGAACCTTGCTGATGATCTCTTGAAGTCCCTGCAGCTTGTCTTGAAGCTTGATGCTCGGGTTGCTGATGCTTACTGTGAGCAAATTACACAGGAAGTCAGTCGCCTTGTGAAAGCAAATGCCTCTCACATCAGATCCCATGGGGGGTGGCGCACAATTACATCCTTACTCTCTATCACAGCCCGGCACCCTGAAGCTTCTGAGTCTGGGTTTGACGCACTGTTCTTCATTATGTCAGAAGGAACCCACTTGTTGCCGACCAACTATGCTCTCTGTGTTGATGCTTCGAGGCAGTTTGCAGAATCTCGCGTTGGGCAGGCAGAACGATCTGTTTGTGCACTTGATCTTATAGCAGGTTCTGTTGATTGTTTAGCAAGGTGGGCCCGTGAGGCTAAGCAAGCTGTGAACGAGGATGAAGCGGTGAAAATGTCTCTGGATATTGGGGAAATGTGGTTCCGACTTGTTCAGGCACTAAGAAAAGTTTGTTTGGACCAGAGAGAAGATGTCAGGAATCATGCTTTGTCTTTGTTGCAGAAGTGCTTGACTGGAGTGGATGGGATCCCTCTTCCACATGGTGTGTGGTTACAGTGCTTTGATGTGGTGATTTTCACAATGCTTGACGACTTGCTTGAAATTGCACAGGGACACTCCCAAAAGGACTACCGCAACATGGAAGGCACACTTATCCTTGCCATGAAGCTCTTGTCCAAGGTGTTCTTACAGCTACTTCCTGGCCTATCACAGTTAACAACCTTCTGCAAACTATGGTTGGGTGTTCTCAGTCGAATGGAAAAGTATATGAAGGTGAAAGTTAGAGGGAAAAAAAGTGAGAAGCTTCAGGACCAAGTACCCGAGCTACTTAAGAATACCTTGCTTGTGATGAATTTAAAGGGAGTGCTCGTGCAAAGGAGCGCTCTAGGAGGAGATAGCTTGTGGGAACTGACATGGCTACATGTCAATAACATTGCGCCATCCTTGCAATCTGAGGTTTTCCCTGATCAAGGTTCAGAACAGTCCGCGACTAAGCTGGGTGAAAACGGAGGTCTAGTTTCTGAAGAAAAGGGTAATGTACTTCCAACTGAAATGGCCTCGACAGAAGTTTCTGGCACTGCAGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Sec7 Sec7_N

Protein Analysis

1470

Amino Acids

163.97

Weight (kDa)

5.65

Isoelectric Point (pI)

50.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec7-like_HUS	PF12783	318 - 480	5.1e-40	Mon2/Sec7/BIG1-like, HUS domain
Sec7	PF01369	565 - 748	7e-71	Sec7 domain
TPR_28	PF23325	1247 - 1400	8.2e-15	GBF1-like, tetratrico peptide repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001807)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13980 AT1G13980
fragaria_vesca	FvH4_7g07780 FvH4_7g07780 FvH4_7g07780 FvH4_7g07780 FvH4_7g07780 FvH4_7g22980 FvH4_7g22980 FvH4_7g22980 FvH4_7g22980 FvH4_7g22980 FvH4_7g22990
malus_domestica	MD01G1138600.v1.1 MD01G1138700.v1.1 MD07G1202800.v1.1
prunus_persica	Prupe.2G239100_v2.0.a1 Prupe.2G239100_v2.0.a1 Prupe.2G239200_v2.0.a1 Prupe.2G239200_v2.0.a1 Prupe.2G239200_v2.0.a1
pyrus_communis	pycom01g15930 pycom01g15940 pycom07g18980 pycom07g18990
rosa_chinensis	RchiOBHm_Chr1g0368181 RchiOBHm_Chr1g0368191
rosa_laevigata	RLG00000027231 RLG00000027232
rosa_multiflora	Rmu_sc0006033.1_g000001 Rmu_sc0006033.1_g000003
rosa_roxburghii	Rroxscaffold_4G00288810
rosa_rugosa	Rorug01G0339200 Rorug01G0339200 Rorug01G0339200
rosa_samantha	Rh1AG346700 Rh1AG346800 Rh1BG309400 Rh1BG309500 Rh1CG324800 Rh1CG324900 Rh1DG341300 Rh1DG341400
rosa_wichuraiana	Rw1G030820 Rw1G030830 Rw1G030840 Rw1G030850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	354
Acc16I	TGCGCA	2 cut(s)	221, 1250
Acc36I	ACCTGC	2 cut(s)	3635, 4394
Acc65I	GGTACC	2 cut(s)	952, 1907
AccB1I	GGYRCC	3 cut(s)	952, 1907, 3477
AccB7I	CCANNNNNTGG	1 cut(s)	329
AccI	GTMKAC	2 cut(s)	772, 1610
AccII	CGCG	3 cut(s)	3042, 3600, 4310
AciI	CCGC	5 cut(s)	128, 3274, 3710, 3955, 4308
AcoI	YGGCCR	2 cut(s)	1551, 2742
AcsI	RAATTY	7 cut(s)	228, 385, 1385, 1898, 2201, 2806, 4174
AcuI	CTGAAG	5 cut(s)	834, 2942, 3504, 4112, 4365
AcyI	GRCGYC	1 cut(s)	7
AdeI	CACNNNGTG	2 cut(s)	1799, 3866
AfeI	AGCGCT	1 cut(s)	4204
AfiI	CCNNNNNNNGG	9 cut(s)	329, 1742, 2261, 2292, 3074, 3075, 3435, 3961, 4292
AflII	CTTAAG	2 cut(s)	2704, 4151
AflIII	ACRYGT	1 cut(s)	4243
AhlI	ACTAGT	1 cut(s)	434
AjnI	CCWGG	6 cut(s)	855, 865, 1975, 2517, 2788, 4021
AjuI	GAANNNNNNNTTGG	2 cut(s)	3585, 3617
AleI	CACNNNNGTG	1 cut(s)	563
AloI	GAACNNNNNNTCC	2 cut(s)	3728, 3760
Alw21I	GWGCWC	7 cut(s)	322, 703, 989, 3192, 3232, 3631, 4191
Alw26I	GTCTC	3 cut(s)	1955, 2891, 3726
Alw44I	GTGCAC	3 cut(s)	318, 699, 3627
AlwNI	CAGNNNCTG	4 cut(s)	1580, 2789, 3277, 3650
Ama87I	CYCGRG	4 cut(s)	50, 2196, 3348, 4142
Aor51HI	AGCGCT	1 cut(s)	4204
ApaI	GGGCCC	2 cut(s)	2396, 3676
ApaLI	GTGCAC	3 cut(s)	318, 699, 3627
ApoI	RAATTY	7 cut(s)	228, 385, 1385, 1898, 2201, 2806, 4174
AseI	ATTAAT	2 cut(s)	261, 1083
Asp700I	GAANNNNTTC	3 cut(s)	972, 1158, 1385
Asp718I	GGTACC	2 cut(s)	952, 1907
AspA2I	CCTAGG	1 cut(s)	2846
AspLEI	GCGC	7 cut(s)	222, 1251, 2071, 3044, 3444, 4205, 4261
AspS9I	GGNCC	8 cut(s)	1022, 2392, 2393, 2786, 3672, 3673, 3781, 4132
AsuC2I	CCSGG	1 cut(s)	3475
AsuII	TTCGAA	1 cut(s)	1162
AsuNHI	GCTAGC	1 cut(s)	2590
AvaI	CYCGRG	4 cut(s)	50, 2196, 3348, 4142
AvaII	GGWCC	3 cut(s)	1022, 3781, 4132
AvrII	CCTAGG	1 cut(s)	2846
AxyI	CCTNAGG	1 cut(s)	3090
BaeGI	GKGCMC	5 cut(s)	322, 703, 2396, 3631, 3676
BaeI	ACNNNNGTAYC	2 cut(s)	3093, 3126
BalI	TGGCCA	1 cut(s)	2744
BamHI	GGATCC	2 cut(s)	1106, 3849
BanI	GGYRCC	3 cut(s)	952, 1907, 3477
BanII	GRGCYC	2 cut(s)	2396, 3676
BauI	CACGAG	2 cut(s)	2736, 4190
BbsI	GAAGAC	1 cut(s)	1491
Bbv12I	GWGCWC	7 cut(s)	322, 703, 989, 3192, 3232, 3631, 4191
BceAI	ACGGC	1 cut(s)	2462
BcgI	CGANNNNNNTGC	4 cut(s)	2360, 2394, 3086, 3120
BciT130I	CCWGG	6 cut(s)	857, 867, 1977, 2519, 2790, 4023
BciVI	GTATCC	2 cut(s)	1930, 3094
BclI	TGATCA	5 cut(s)	178, 379, 1717, 2425, 4288
BcnI	CCSGG	1 cut(s)	3475
BcoDI	GTCTC	3 cut(s)	1955, 2891, 3726
BcuI	ACTAGT	1 cut(s)	434
BfoI	RGCGCY	1 cut(s)	4206
BfrI	CTTAAG	2 cut(s)	2704, 4151
BfuAI	ACCTGC	2 cut(s)	3635, 4394
BfuI	GTATCC	2 cut(s)	1930, 3094
BglI	GCCNNNNNGGC	1 cut(s)	3680
BglII	AGATCT	2 cut(s)	302, 1028
BlnI	CCTAGG	1 cut(s)	2846
BlpI	GCTNAGC	1 cut(s)	3684
BmcAI	AGTACT	1 cut(s)	718
Bme1390I	CCNGG	7 cut(s)	857, 867, 1977, 2519, 2790, 3475, 4023
Bme18I	GGWCC	3 cut(s)	1022, 3781, 4132
BmeT110I	CYCGRG	4 cut(s)	50, 2196, 3348, 4142
BmgT120I	GGNCC	8 cut(s)	1022, 2392, 2393, 2786, 3672, 3673, 3781, 4132
BmrFI	CCNGG	7 cut(s)	857, 867, 1977, 2519, 2790, 3475, 4023
BmtI	GCTAGC	1 cut(s)	2594
BpiI	GAAGAC	1 cut(s)	1491
BpmI	CTGGAG	5 cut(s)	263, 1769, 1998, 2238, 3858
Bpu1102I	GCTNAGC	1 cut(s)	3684
Bpu14I	TTCGAA	1 cut(s)	1162
BpuEI	CTTGAG	2 cut(s)	1292, 2915
BpuMI	CCSGG	1 cut(s)	3475
BsaAI	YACGTR	1 cut(s)	573
BsaHI	GRCGYC	1 cut(s)	7
BsaJI	CCNNGG	8 cut(s)	51, 866, 1071, 1759, 2789, 2846, 3430, 3999
BsaWI	WCCGGW	1 cut(s)	170
BsaXI	ACNNNNNCTCC	6 cut(s)	817, 847, 4176, 4206, 4323, 4353
Bsc4I	CCNNNNNNNGG	9 cut(s)	329, 1742, 2261, 2292, 3074, 3075, 3435, 3961, 4292
Bse118I	RCCGGY	2 cut(s)	1904, 2292
Bse1I	ACTGG	8 cut(s)	28, 202, 319, 334, 1592, 1752, 2745, 3841
Bse21I	CCTNAGG	1 cut(s)	3090
Bse3DI	GCAATG	3 cut(s)	215, 1441, 4254
BseBI	CCWGG	6 cut(s)	857, 867, 1977, 2519, 2790, 4023
BseDI	CCNNGG	8 cut(s)	51, 866, 1071, 1759, 2789, 2846, 3430, 3999
BseLI	CCNNNNNNNGG	9 cut(s)	329, 1742, 2261, 2292, 3074, 3075, 3435, 3961, 4292
BseMI	GCAATG	3 cut(s)	215, 1441, 4254
BseMII	CTCAG	8 cut(s)	891, 930, 996, 2031, 3081, 3483, 4084, 4266
BseNI	ACTGG	8 cut(s)	28, 202, 319, 334, 1592, 1752, 2745, 3841
BseRI	GAGGAG	5 cut(s)	151, 2159, 2594, 2918, 4227
BseSI	GKGCMC	5 cut(s)	322, 703, 2396, 3631, 3676
BseYI	CCCAGC	3 cut(s)	740, 1826, 4318
BsgI	GTGCAG	2 cut(s)	1566, 1926
Bsh1236I	CGCG	3 cut(s)	3042, 3600, 4310
BshNI	GGYRCC	3 cut(s)	952, 1907, 3477
BsiHKAI	GWGCWC	7 cut(s)	322, 703, 989, 3192, 3232, 3631, 4191
BsiHKCI	CYCGRG	4 cut(s)	50, 2196, 3348, 4142
BsiSI	CCGG	5 cut(s)	171, 1678, 1905, 2293, 3475
BslFI	GGGAC	3 cut(s)	1035, 3304, 3948
BslI	CCNNNNNNNGG	9 cut(s)	329, 1742, 2261, 2292, 3074, 3075, 3435, 3961, 4292
BsmAI	GTCTC	3 cut(s)	1955, 2891, 3726
BsmFI	GGGAC	3 cut(s)	1035, 3304, 3948
BsmI	GAATGC	2 cut(s)	1475, 2012
BsoBI	CYCGRG	4 cut(s)	50, 2196, 3348, 4142
Bsp119I	TTCGAA	1 cut(s)	1162
Bsp120I	GGGCCC	2 cut(s)	2392, 3672
Bsp1286I	GDGCHC	9 cut(s)	322, 703, 989, 2396, 3192, 3232, 3631, 3676, 4191
Bsp1720I	GCTNAGC	1 cut(s)	3684
Bsp19I	CCATGG	1 cut(s)	3430
BspACI	CCGC	5 cut(s)	128, 3274, 3710, 3955, 4308
BspCNI	CTCAG	8 cut(s)	892, 931, 997, 2030, 3082, 3484, 4083, 4267
BspFNI	CGCG	3 cut(s)	3042, 3600, 4310
BspHI	TCATGA	2 cut(s)	933, 1867
BspMAI	CTGCAG	6 cut(s)	1376, 3015, 3061, 3109, 3327, 4405
BspMI	ACCTGC	2 cut(s)	3635, 4394
BspOI	GCTAGC	1 cut(s)	2594
BspT104I	TTCGAA	1 cut(s)	1162
BspT107I	GGYRCC	3 cut(s)	952, 1907, 3477
BspTI	CTTAAG	2 cut(s)	2704, 4151
BsrDI	GCAATG	3 cut(s)	215, 1441, 4254
BsrFI	RCCGGY	2 cut(s)	1904, 2292
BsrI	ACTGG	8 cut(s)	28, 202, 319, 334, 1592, 1752, 2745, 3841
BssAI	RCCGGY	2 cut(s)	1904, 2292
BssECI	CCNNGG	8 cut(s)	51, 866, 1071, 1759, 2789, 2846, 3430, 3999
BssNAI	GTATAC	1 cut(s)	1611
BssNI	GRCGYC	1 cut(s)	7
BssSI	CACGAG	2 cut(s)	2736, 4190
BssT1I	CCWWGG	5 cut(s)	1071, 1759, 2846, 3430, 3999
Bst1107I	GTATAC	1 cut(s)	1611
Bst2BI	CACGAG	2 cut(s)	2736, 4190
Bst2UI	CCWGG	6 cut(s)	857, 867, 1977, 2519, 2790, 4023
Bst6I	CTCTTC	5 cut(s)	512, 1599, 2436, 2821, 3861
BstACI	GRCGYC	1 cut(s)	7
BstAFI	CTTAAG	2 cut(s)	2704, 4151
BstBAI	YACGTR	1 cut(s)	573
BstBI	TTCGAA	1 cut(s)	1162
BstDSI	CCRYGG	1 cut(s)	3430
BstEII	GGTNACC	1 cut(s)	451
BstENI	CCTNNNNNAGG	1 cut(s)	4290
BstFNI	CGCG	3 cut(s)	3042, 3600, 4310
BstH2I	RGCGCY	1 cut(s)	4206
BstHHI	GCGC	7 cut(s)	222, 1251, 2071, 3044, 3444, 4205, 4261
BstMAI	GTCTC	3 cut(s)	1955, 2891, 3726
BstNI	CCWGG	6 cut(s)	857, 867, 1977, 2519, 2790, 4023
BstNSI	RCATGY	5 cut(s)	96, 554, 1431, 2499, 4247
BstPI	GGTNACC	1 cut(s)	451
BstSCI	CCNGG	7 cut(s)	855, 865, 1975, 2517, 2788, 3473, 4021
BstSLI	GKGCMC	5 cut(s)	322, 703, 2396, 3631, 3676
BstUI	CGCG	3 cut(s)	3042, 3600, 4310
BstV2I	GAAGAC	1 cut(s)	1491
BstX2I	RGATCY	7 cut(s)	302, 1028, 1106, 2241, 2852, 3425, 3849
BstXI	CCANNNNNNTGG	1 cut(s)	2295
BstYI	RGATCY	7 cut(s)	302, 1028, 1106, 2241, 2852, 3425, 3849
BstZ17I	GTATAC	1 cut(s)	1611
Bsu36I	CCTNAGG	1 cut(s)	3090
BsuI	GTATCC	2 cut(s)	1930, 3094
BtgI	CCRYGG	1 cut(s)	3430
BtsI	GCAGTG	3 cut(s)	1821, 2191, 4398
BveI	ACCTGC	2 cut(s)	3635, 4394
CaiI	CAGNNNCTG	4 cut(s)	1580, 2789, 3277, 3650
CciI	TCATGA	2 cut(s)	933, 1867
CfoI	GCGC	7 cut(s)	222, 1251, 2071, 3044, 3444, 4205, 4261
Cfr10I	RCCGGY	2 cut(s)	1904, 2292
Cfr13I	GGNCC	8 cut(s)	1022, 2392, 2393, 2786, 3672, 3673, 3781, 4132
CseI	GACGC	1 cut(s)	3515
DraI	TTTAAA	3 cut(s)	210, 2562, 4179
DraIII	CACNNNGTG	2 cut(s)	1799, 3866
EaeI	YGGCCR	2 cut(s)	1551, 2742
Eam1104I	CTCTTC	5 cut(s)	512, 1599, 2436, 2821, 3861
EarI	CTCTTC	5 cut(s)	512, 1599, 2436, 2821, 3861
Eco130I	CCWWGG	5 cut(s)	1071, 1759, 2846, 3430, 3999
Eco147I	AGGCCT	2 cut(s)	855, 1277
Eco24I	GRGCYC	2 cut(s)	2396, 3676
Eco32I	GATATC	1 cut(s)	2490
Eco47I	GGWCC	3 cut(s)	1022, 3781, 4132
Eco47III	AGCGCT	1 cut(s)	4204
Eco57I	CTGAAG	5 cut(s)	834, 2942, 3504, 4112, 4365
Eco81I	CCTNAGG	1 cut(s)	3090
Eco88I	CYCGRG	4 cut(s)	50, 2196, 3348, 4142
Eco91I	GGTNACC	1 cut(s)	451
EcoNI	CCTNNNNNAGG	1 cut(s)	4290
EcoO109I	RGGNCCY	3 cut(s)	1022, 2393, 2786
EcoO65I	GGTNACC	1 cut(s)	451
EcoRII	CCWGG	6 cut(s)	855, 865, 1975, 2517, 2788, 4021
EcoRV	GATATC	1 cut(s)	2490
EcoT14I	CCWWGG	5 cut(s)	1071, 1759, 2846, 3430, 3999
EcoT22I	ATGCAT	2 cut(s)	98, 645
EcoT38I	GRGCYC	2 cut(s)	2396, 3676
ErhI	CCWWGG	5 cut(s)	1071, 1759, 2846, 3430, 3999
FalI	AAGNNNNNCTT	8 cut(s)	3282, 3314, 3957, 3989, 3992, 4024, 4145, 4177
FaqI	GGGAC	3 cut(s)	1035, 3304, 3948
FauNDI	CATATG	2 cut(s)	946, 1011
FbaI	TGATCA	5 cut(s)	178, 379, 1717, 2425, 4288
FblI	GTMKAC	2 cut(s)	772, 1610
FriOI	GRGCYC	2 cut(s)	2396, 3676
FspI	TGCGCA	2 cut(s)	221, 1250
GlaI	GCGC	7 cut(s)	221, 1250, 2070, 3043, 3443, 4204, 4260
GsaI	CCCAGC	3 cut(s)	744, 1830, 4322
GsuI	CTGGAG	5 cut(s)	263, 1769, 1998, 2238, 3858
HaeII	RGCGCY	1 cut(s)	4206
HapII	CCGG	5 cut(s)	171, 1678, 1905, 2293, 3475
HgaI	GACGC	1 cut(s)	3515
HhaI	GCGC	7 cut(s)	222, 1251, 2071, 3044, 3444, 4205, 4261
Hin1I	GRCGYC	1 cut(s)	7
Hin6I	GCGC	7 cut(s)	220, 1249, 2069, 3042, 3442, 4203, 4259
HinP1I	GCGC	7 cut(s)	220, 1249, 2069, 3042, 3442, 4203, 4259
HincII	GTYRAC	5 cut(s)	815, 1188, 1366, 3213, 4038
HindII	GTYRAC	5 cut(s)	815, 1188, 1366, 3213, 4038
HindIII	AAGCTT	4 cut(s)	2717, 3338, 3486, 4124
HpaI	GTTAAC	1 cut(s)	4038
HpaII	CCGG	5 cut(s)	171, 1678, 1905, 2293, 3475
HpyCH4IV	ACGT	3 cut(s)	86, 572, 697
HpySE526I	ACGT	3 cut(s)	86, 572, 697
Hsp92I	GRCGYC	1 cut(s)	7
HspAI	GCGC	7 cut(s)	220, 1249, 2069, 3042, 3442, 4203, 4259
KflI	GGGWCCC	1 cut(s)	1022
KpnI	GGTACC	2 cut(s)	956, 1911
Ksp22I	TGATCA	5 cut(s)	178, 379, 1717, 2425, 4288
KspAI	GTTAAC	1 cut(s)	4038
LmnI	GCTCC	6 cut(s)	1322, 1712, 2335, 2581, 2905, 4200
MaeII	ACGT	3 cut(s)	86, 572, 697
MaeIII	GTNAC	7 cut(s)	451, 542, 1418, 1634, 1730, 2629, 3873
MfeI	CAATTG	5 cut(s)	36, 776, 1486, 2400, 2643
MflI	RGATCY	7 cut(s)	302, 1028, 1106, 2241, 2852, 3425, 3849
MhlI	GDGCHC	9 cut(s)	322, 703, 989, 2396, 3192, 3232, 3631, 3676, 4191
MlsI	TGGCCA	1 cut(s)	2744
MluNI	TGGCCA	1 cut(s)	2744
MlyI	GAGTC	3 cut(s)	1201, 2771, 3503
MmeI	TCCRAC	7 cut(s)	1812, 1972, 2660, 2745, 3222, 3772, 4391
Mox20I	TGGCCA	1 cut(s)	2744
Mph1103I	ATGCAT	2 cut(s)	98, 645
MroXI	GAANNNNTTC	3 cut(s)	972, 1158, 1385
MscI	TGGCCA	1 cut(s)	2744
MslI	CAYNNNNRTG	2 cut(s)	563, 2175
Msp20I	TGGCCA	1 cut(s)	2744
MspA1I	CMGCKG	3 cut(s)	184, 2777, 3274
MspCI	CTTAAG	2 cut(s)	2704, 4151
MspI	CCGG	5 cut(s)	171, 1678, 1905, 2293, 3475
MspR9I	CCNGG	7 cut(s)	857, 867, 1977, 2519, 2790, 3475, 4023
MunI	CAATTG	5 cut(s)	36, 776, 1486, 2400, 2643
Mva1269I	GAATGC	2 cut(s)	1475, 2012
MvaI	CCWGG	6 cut(s)	857, 867, 1977, 2519, 2790, 4023
MvnI	CGCG	3 cut(s)	3042, 3600, 4310
NciI	CCSGG	1 cut(s)	3475
NcoI	CCATGG	1 cut(s)	3430
NdeI	CATATG	2 cut(s)	946, 1011
NheI	GCTAGC	1 cut(s)	2590
NmuCI	GTSAC	5 cut(s)	451, 1418, 1634, 1730, 2629
NsbI	TGCGCA	2 cut(s)	221, 1250
NsiI	ATGCAT	2 cut(s)	98, 645
NspI	RCATGY	5 cut(s)	96, 554, 1431, 2499, 4247
NspV	TTCGAA	1 cut(s)	1162
OliI	CACNNNNGTG	1 cut(s)	563
PaeI	GCATGC	2 cut(s)	96, 2499
PaeR7I	CTCGAG	1 cut(s)	2196
PagI	TCATGA	2 cut(s)	933, 1867
PceI	AGGCCT	2 cut(s)	855, 1277
PciI	ACATGT	1 cut(s)	4243
PctI	GAATGC	2 cut(s)	1475, 2012
PdmI	GAANNNNTTC	3 cut(s)	972, 1158, 1385
PfeI	GAWTC	8 cut(s)	1120, 1597, 1840, 1982, 2348, 3020, 3593, 3802
PflMI	CCANNNNNTGG	1 cut(s)	329
PfoI	TCCNGGA	1 cut(s)	2517
PleI	GAGTC	3 cut(s)	1200, 2770, 3502
PpsI	GAGTC	3 cut(s)	1200, 2770, 3502
Ppu21I	YACGTR	1 cut(s)	573
PpuMI	RGGWCCY	1 cut(s)	1022
PscI	ACATGT	1 cut(s)	4243
PshBI	ATTAAT	2 cut(s)	261, 1083
PsiI	TTATAA	1 cut(s)	354
Psp5II	RGGWCCY	1 cut(s)	1022
Psp6I	CCWGG	6 cut(s)	855, 865, 1975, 2517, 2788, 4021
PspEI	GGTNACC	1 cut(s)	451
PspFI	CCCAGC	3 cut(s)	740, 1826, 4318
PspGI	CCWGG	6 cut(s)	855, 865, 1975, 2517, 2788, 4021
PspOMI	GGGCCC	2 cut(s)	2392, 3672
PspPI	GGNCC	8 cut(s)	1022, 2392, 2393, 2786, 3672, 3673, 3781, 4132
PspPPI	RGGWCCY	1 cut(s)	1022
PsrI	GAACNNNNNNTAC	2 cut(s)	1248, 1280
PstI	CTGCAG	6 cut(s)	1376, 3015, 3061, 3109, 3327, 4405
PstNI	CAGNNNCTG	4 cut(s)	1580, 2789, 3277, 3650
PsuI	RGATCY	7 cut(s)	302, 1028, 1106, 2241, 2852, 3425, 3849
PvuII	CAGCTG	2 cut(s)	184, 2777
RseI	CAYNNNNRTG	2 cut(s)	563, 2175
Sau96I	GGNCC	8 cut(s)	1022, 2392, 2393, 2786, 3672, 3673, 3781, 4132
ScaI	AGTACT	1 cut(s)	718
SchI	GAGTC	3 cut(s)	1201, 2771, 3503
ScrFI	CCNGG	7 cut(s)	857, 867, 1977, 2519, 2790, 3475, 4023
SduI	GDGCHC	9 cut(s)	322, 703, 989, 2396, 3192, 3232, 3631, 3676, 4191
Sfr274I	CTCGAG	1 cut(s)	2196
SfuI	TTCGAA	1 cut(s)	1162
SinI	GGWCC	3 cut(s)	1022, 3781, 4132
SlaI	CTCGAG	1 cut(s)	2196
SmiMI	CAYNNNNRTG	2 cut(s)	563, 2175
SmlI	CTYRAG	5 cut(s)	1271, 2196, 2704, 2894, 4151
SmoI	CTYRAG	5 cut(s)	1271, 2196, 2704, 2894, 4151
SpeI	ACTAGT	1 cut(s)	434
SphI	GCATGC	2 cut(s)	96, 2499
SseBI	AGGCCT	2 cut(s)	855, 1277
SsiI	CCGC	5 cut(s)	128, 3274, 3710, 3955, 4308
SspI	AATATT	2 cut(s)	972, 2689
StuI	AGGCCT	2 cut(s)	855, 1277
StyD4I	CCNGG	7 cut(s)	855, 865, 1975, 2517, 2788, 3473, 4021
StyI	CCWWGG	5 cut(s)	1071, 1759, 2846, 3430, 3999
TaiI	ACGT	3 cut(s)	89, 575, 700
TaqI	TCGA	7 cut(s)	442, 1162, 1969, 2197, 3578, 4075, 4382
TatI	WGTACW	4 cut(s)	716, 883, 1686, 4360
TauI	GCSGC	1 cut(s)	3277
TfiI	GAWTC	8 cut(s)	1120, 1597, 1840, 1982, 2348, 3020, 3593, 3802
TseFI	GTSAC	5 cut(s)	451, 1418, 1634, 1730, 2629
Tsp45I	GTSAC	5 cut(s)	451, 1418, 1634, 1730, 2629
TspGWI	ACGGA	3 cut(s)	379, 2156, 4344
Van91I	CCANNNNNTGG	1 cut(s)	329
Vha464I	CTTAAG	2 cut(s)	2704, 4151
VneI	GTGCAC	3 cut(s)	318, 699, 3627
VpaK11BI	GGWCC	3 cut(s)	1022, 3781, 4132
VspI	ATTAAT	2 cut(s)	261, 1083
XagI	CCTNNNNNAGG	1 cut(s)	4290
XapI	RAATTY	7 cut(s)	228, 385, 1385, 1898, 2201, 2806, 4174
XceI	RCATGY	5 cut(s)	96, 554, 1431, 2499, 4247
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3867
XhoI	CTCGAG	1 cut(s)	2196
XmaJI	CCTAGG	1 cut(s)	2846
XmiI	GTMKAC	2 cut(s)	772, 1610
XmnI	GAANNNNTTC	3 cut(s)	972, 1158, 1385
ZrmI	AGTACT	1 cut(s)	718
Zsp2I	ATGCAT	2 cut(s)	98, 645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.