MD07G1202800.v1.1

ARF guanine-nucleotide exchange factor GNOM-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001807
Physical Location & Seq
Reverse (-)
28079930 .. 28086050
6121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1202800.v1.1.491

Sequence Viewer

Length: 4413 bp
ATGGGGCGTCTGAAGCTGCGAAGTGGAATCCAGGAAATTGAGGAAGAACATGGGGAATGTGATGCAACGCATTCTAATCATGCTACTTTAGCATGCATCATTAATTCAGAAATAGGCACTGTATTAGCGGTTATGAGGAGGAATGTAAGATGGGGAGGACGCTATGTGTCTGGTGATGATCAGCTGGAACACCCTCTAATCCAATCTTTAAAGGCGTTACGCAAGCAAATCTTTTCATGGAAGCACCATATGCATTCCGTCAATCCTTCTGCATATCTCCGGCCATTTTTGGATGTGATTAGGTCGGATGAAACTGGTGCACCAATTACTGGTGTGGCTTTGTCTTCTGTTTACAATATCTTAACACTTGATGTAATCAATCAAAATTCCTTAAATGTTGAAGATGCCATGCACTTATTAGTTGATGCAATCACTGGCTGCCGATTTGAGGTGACTGATCCTGCATCAGAAGAAGTGGTATTGATGAAGATACTGCAGGTTCTTCTAGCTTGCATGAAGAGTAAAGCATCTGTTATATTGACCAATCAGCATGTTTGCATTATAGTGAACACATGTTTCCGTATTGTCCATCAAGCGGGAACAAAAGGTGAGTTGTTGCAGCGGATAGCTCGCCACACAATGCATGAACTTGTTAGATGTATTTTCTCACACCTTCCAGATGTTCACCATTCTGAACATGCATTGCCAAATGGAAGCAATACCATCAAGGGAGAGATTGCAGGGCAAAATAATGAGCATGCTTCTGGGAGTAGACAATTGGAGAATGGCAACATGATTTCTGAGTTTGATAGTCAGCTATTATCTTCAAATCCTGCTTTGCATGCTTCCTCAGGTCTTGTAGAATCTGGGATTGATGAAAAGACAGCTGGTGGTTCTAGCAGGAAGGAGGCTGATCAATATGACGTGCAACTCATGGCTGAGCCATTTGGGATTCCCTGCATGGTGGAAATATTGGACTTCCTGTGTTCTTTGTTAAATGTGGTTGAGCATATAGGAATGGGTCCCAGGTATAATACCATAGCTTTTGACGAAGATGTCCCCCTTTTTGCACTGGGTTTGGTTAATTGCGCTATAGAGTTAGGCGGGTCCTCCATTCGGCATCACCCCAAGTTATTAAGTTTGGTTCAGGATGAATTGTTTCAAAATTTGATGCAGTTTGGCCTATCAACAAGTCCACTTATTCTTTCAATGGTATGCAGCATTGTCCTCAATCTGTATCACCATCTCCGCACTGAACTTAAGCTACAGCTTGAGGCTTTCTTTTCATGTGTGATATTGAGGCTTGTGCAAAGCAGGTATGGGGCTTCTTATCAGCAGCAGGAGGTTGCCATGGAGGCTGTTGTGGACTTCTGCCGGCAGAAAACTTTCATGGTGGAGATGTACGCGAACTTAGATTGCGACATAACTTGCAATAATGTCTTTGAAGAGCTTGCTAATCTGTTGTCAAAGAGTGCATTCCCTGTTAATTTGCTTTTGTCTTCAATTCACATTCTTGCTTTGGATGGCCTTATTGCTATTATTCAGGGAATGGCAGAGAGGGCAGGGAAGGGCTCAGTTAGTGCTGTTCAGGCTCCCCCTATGAATCTTGATGAGTATACTCCATTCTGGTTGATGAAATGTGACAACTACAGTGATCCGAATCATTGGGTTCCATTTGTTCGCCGAAGGAAGCACATAAAGAGAAGGCTAATGATTGGAGCCGATCATTTTAACCATGACCCAAAGAAAGGGCTGGAGTTCCTCCAAGGAACCCATCTCTTGCCTGACAAACTTGATCCTGAAAGTGTGGCCTGTTTTTTCAGGTACACTGCTGGGTTGGACAAGAATCTTGTTGGGGATTTCCTGGGAAATCATGATGAGTTTTGTATTCAGGTTCTTAATAAATTTGCTGGTACCTTTGTTTTCCAAGACATGCATTTGGATACTGCACTGCGACTGTTTCTGGAGACATTTCGATTGCCTGGAGAATCACAAAAAATACAAAGGGTGCTTGAAGCATTCTCAGAGAGATATTATGAGCAATCACCACAAATTCTAGCTAACAAGGATGCTGCTCTCCTGTTATCATATTCAATTATATTGCTCAACACAGACCAGCACAATGTTCAGGTGAAGAAGAAGATGACAGAGGAGGATTTCATTCGGAATAATCGACACATCAATGGAGGTGATGATTTACCTCTAGAATTCCTTTCAGAGCTTTACCACTCAATATGCAAGAATGAGATCCGCACAACTCCAGAACAAGGTGCTGGTTATTCTGAAATGAACCCAAGCCGTTGGATTGATTTAATACACAAATCTAAGAAGAATGCTCCCTTCATTGAATCCGATACCAGAGCCTACCTTGACCAGGATATGTTTGCTATAATGTCAGGCCCCACAATTGCTGCCATTTCTGTGGTATTTGACCATGCAGAACATGAAGAGATTTACCAAAAATGCATTGATGGATTTTTATATGTTGCAAAGATTGCAGCATGCTATCATTTTGAAGATGTGCTCGACGATCTGGTTGTGTCTCTTTGTAAGTTTACAACCCTTTTAAACCCATCATCTTATGAGGAACCTGTTTTAGCCTTTGGTGATGACCCAAAAGCTAGAATGTCAACAGTGACAGTTTTCACGATTGCCAATACATATGGTGATCACATTCGCACAGGCTGGAGAAATGTTCTTGACTGTATCTTAAGATTACACAAACTTGGTCTTCTGTCGGCTTTTGTGGCCAGTGAAGCAGCTGGTGATTCTGAGTTTTCTGCTGACACAGGTCATGGCAAGGCCATAACCAATTCTCTGTCATCCGTTCCTATGCCCCCTCTGAGTACTTCTAGGAAGTCCTCTGGATTGATGGGCCGGTTTACTCAGCTATTATCTCTTAACACTGAGGAGCCGAGATCACAGCCTACTGAAGAAGAACTTGTGGCTCATCAGCGCACCCTTCAGACAATTCAAAAGTGCCACATTGATGGCATATTTTCAGAGAGCAAGTTTCTGCAGGCTAAATCCCTGTTACAGCTTGCACAGGCACTCATCTGGGCTGGAGAACGACCCCAGAAAGGGAGCAGCTCACCTGAGGATGAAGATACTGCAGTTTTCTGTTTGGAGTTGCTGATAGCTGTTACCCTAAATAACAGGGATAGGATTATGCTTCTTTGGCAGATTGTGTATGAGCACATATCGAACATTGTTCAGTCTACTGTGATGCCTTGTGCCCTGGTAGAGAAGGCTATTTTTGGACTTCTTCGGATTTGCCAGCGGCTGCTTCCCTATAAAGAGGACCTTGCTGATGATCTTTTGCGATCATTGCAACTTGTTTTGAAGCTTGATGCTCGGGTTGCGGATGCATACTGTGAGCAAATTACGCAGGAAGTCGGTCGCCTTGTGAAAGCAAATGCCTCTCACATAAGATCACAATTGGGGTGGCACATAACTACATCACTACTCTCCATTACTGCTCCGCACCCAGAAGCTTCAGAGGCTGGGTTCGATGCACTGTTCTTCATTATGTCTGAAGGAGCTCATTTGTTGCCTGCCAATTATGTTTTCTGTGTTGACGCATCAAGGCAGTTTGCTGAGTCTCGTGTTGGAGAGGTGGCGCGATCTGTGTGCGCACTGGATCTTATGGCAGGTTCTGTTGATTGTTTATCAAGGTGGGCCTCTGACGCTAACCAATCTATGAATGGGGAAGAAGCTGTGAAAATGTCTCAGGATATTGGGCAAATGTGGTTGCGGCTTGTTCAGGGGTTGAGAAAAGTTTGTTTGGACCAGAGAGAAGAGGTTAGGAACCATGCTTTGTCCATGTTACGGAAATGCTTGACTGGAGTGGATGGGATTCCTCTTCCACATGGCCTGTGGTTACGGTGCTTTGACACGGTGATCTTCACAATGCTTGATGACTTGCTAGAAATTGCACAGGGACAGTCCCCAAAGGAATACCGCTACATGGAAGGCACGCTTATCCTAGCCTTGAAGCTCTTGTCCAAAGTTTTTCTGGAGCTACTACCCGAGCTATCGCAGTTAACAACCTTCAGCAAACTATGGCTGGGTGTTCTCAATCGAATGGAAAATTATATGAAGGTGAAAGTTGGAGGGAGAAAATGTGAGAAGCTACGGGACCAAGTGCTGGAGCTACTTAAGAACACCTTAATCGTAATGATTTTAAGGGGAGTTCTTTTGGAGAAAGGTGACGTAGGAGATGATAACTTGTGGGAGTTGACATGGCTGCATGTAAATAAAATTGCTCCATCCTTGCAGTCTGAAGTTTCCCGTGATCCATTTTTAGAGCAGTCAGAGACTAAGCAGGGTGAATCAGGGCGAGTCTCTGATGCAACGGGCACCATACTTCCAACTGAAACCATTCCTGCTGAAGGTTCCGGTGGTGGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Sec7 Sec7_N

Protein Analysis

1471

Amino Acids

164.26

Weight (kDa)

5.57

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 318 - 480 1.7e-37 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 567 - 750 2.4e-69 Sec7 domain
TPR_28 PF23325 1249 - 1429 5.8e-11 GBF1-like, tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1055
Acc16I TGCGCA 1 cut(s) 3634
Acc36I ACCTGC 3 cut(s) 487, 1305, 3641
Acc65I GGTACC 1 cut(s) 1913
AccB1I GGYRCC 2 cut(s) 1913, 4358
AccB7I CCANNNNNTGG 2 cut(s) 329, 4147
AccI GTMKAC 3 cut(s) 772, 1616, 3218
AccII CGCG 2 cut(s) 1406, 3622
AclWI GGATC 6 cut(s) 452, 1649, 1790, 2242, 3648, 4289
AcoI YGGCCR 2 cut(s) 281, 2748
AcsI RAATTY 5 cut(s) 385, 1165, 1904, 2052, 2207
AcuI CTGAAG 8 cut(s) 32, 2948, 2952, 3480, 3555, 4036, 4302, 4410
AcyI GRCGYC 1 cut(s) 7
AfaI GTAC 4 cut(s) 1403, 1826, 1915, 2848
AflII CTTAAG 3 cut(s) 1259, 2710, 4157
AflIII ACRYGT 1 cut(s) 572
AjiI CACGTC 1 cut(s) 925
AjnI CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3237
AjuI GAANNNNNNNTTGG 2 cut(s) 3551, 3583
Alw21I GWGCWC 4 cut(s) 322, 2526, 3198, 3544
Alw26I GTCTC 6 cut(s) 1961, 2547, 3606, 3732, 4310, 4348
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 6 cut(s) 452, 1649, 1790, 2242, 3648, 4289
AlwNI CAGNNNCTG 3 cut(s) 3283, 3503, 3656
Ama87I CYCGRG 2 cut(s) 3354, 4028
ApaLI GTGCAC 1 cut(s) 318
ApoI RAATTY 5 cut(s) 385, 1165, 1904, 2052, 2207
AseI ATTAAT 1 cut(s) 102
Asp700I GAANNNNTTC 3 cut(s) 1158, 1385, 4380
Asp718I GGTACC 1 cut(s) 1913
AspLEI GCGC 4 cut(s) 1091, 2958, 3622, 3635
AspS9I GGNCC 8 cut(s) 1022, 1107, 2399, 2875, 3301, 3678, 3787, 4138
AvaI CYCGRG 2 cut(s) 3354, 4028
AvaII GGWCC 5 cut(s) 1022, 1107, 3301, 3787, 4138
AxyI CCTNAGG 2 cut(s) 850, 3096
BaeGI GKGCMC 3 cut(s) 322, 3238, 4361
BaeI ACNNNNGTAYC 4 cut(s) 1905, 1938, 3099, 3132
BalI TGGCCA 1 cut(s) 2750
BanI GGYRCC 2 cut(s) 1913, 4358
BanII GRGCYC 2 cut(s) 1574, 3544
BauI CACGAG 1 cut(s) 3603
BbsI GAAGAC 3 cut(s) 336, 1491, 2723
Bbv12I GWGCWC 4 cut(s) 322, 2526, 3198, 3544
BceAI ACGGC 1 cut(s) 2283
BcgI CGANNNNNNTGC 2 cut(s) 3612, 3646
BciT130I CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
BciVI GTATCC 1 cut(s) 1936
BclI TGATCA 3 cut(s) 178, 913, 2668
BcoDI GTCTC 6 cut(s) 1961, 2547, 3606, 3732, 4310, 4348
BfaI CTAG 8 cut(s) 506, 897, 2057, 2204, 2622, 2853, 3926, 3986
BfmI CTRYAG 6 cut(s) 494, 1092, 1265, 1648, 3017, 3111
BfrI CTTAAG 3 cut(s) 1259, 2710, 4157
BfuAI ACCTGC 3 cut(s) 487, 1305, 3641
BfuI GTATCC 1 cut(s) 1936
BglI GCCNNNNNGGC 1 cut(s) 1355
BlpI GCTNAGC 1 cut(s) 939
BmcAI AGTACT 1 cut(s) 2848
Bme1390I CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
Bme18I GGWCC 5 cut(s) 1022, 1107, 3301, 3787, 4138
BmeT110I CYCGRG 2 cut(s) 3354, 4028
BmgBI CACGTC 1 cut(s) 925
BmgT120I GGNCC 8 cut(s) 1022, 1107, 2399, 2875, 3301, 3678, 3787, 4138
BmrFI CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
BmrI ACTGGG 1 cut(s) 1082
BmuI ACTGGG 1 cut(s) 1082
BoxI GACNNNNGTC 1 cut(s) 2790
BpiI GAAGAC 3 cut(s) 336, 1491, 2723
BpmI CTGGAG 9 cut(s) 1775, 1985, 2004, 2244, 2707, 3084, 3864, 4037, 4169
Bpu1102I GCTNAGC 1 cut(s) 939
BpuEI CTTGAG 1 cut(s) 1292
BsaBI GATNNNNATC 1 cut(s) 1659
BsaHI GRCGYC 1 cut(s) 7
BsaJI CCNNGG 5 cut(s) 1025, 1350, 1765, 1864, 3237
BsaWI WCCGGW 1 cut(s) 4397
BsaXI ACNNNNNCTCC 2 cut(s) 4108, 4138
Bse118I RCCGGY 2 cut(s) 1374, 2877
Bse1I ACTGG 7 cut(s) 319, 334, 439, 1077, 2751, 3642, 3847
Bse21I CCTNAGG 2 cut(s) 850, 3096
Bse3DI GCAATG 2 cut(s) 701, 3326
Bse8I GATNNNNATC 1 cut(s) 1659
BseBI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
BseDI CCNNGG 5 cut(s) 1025, 1350, 1765, 1864, 3237
BseJI GATNNNNATC 1 cut(s) 1659
BseMI GCAATG 2 cut(s) 701, 3326
BseNI ACTGG 7 cut(s) 319, 334, 439, 1077, 2751, 3642, 3847
BseRI GAGGAG 3 cut(s) 151, 2165, 2924
BseSI GKGCMC 3 cut(s) 322, 3238, 4361
BseYI CCCAGC 3 cut(s) 1832, 3503, 4066
BsgI GTGCAG 1 cut(s) 1932
Bsh1236I CGCG 2 cut(s) 1406, 3622
Bsh1285I CGRYCG 1 cut(s) 3400
BshNI GGYRCC 2 cut(s) 1913, 4358
BsiEI CGRYCG 1 cut(s) 3400
BsiHKAI GWGCWC 4 cut(s) 322, 2526, 3198, 3544
BsiHKCI CYCGRG 2 cut(s) 3354, 4028
BsiSI CCGG 4 cut(s) 280, 1375, 2878, 4398
BslFI GGGAC 5 cut(s) 1008, 1043, 3931, 3954, 4151
BsmAI GTCTC 6 cut(s) 1961, 2547, 3606, 3732, 4310, 4348
BsmFI GGGAC 5 cut(s) 1008, 1043, 3931, 3954, 4151
BsmI GAATGC 5 cut(s) 70, 253, 1475, 2018, 2338
BsoBI CYCGRG 2 cut(s) 3354, 4028
Bsp1286I GDGCHC 7 cut(s) 322, 1574, 2526, 3198, 3238, 3544, 4361
Bsp1720I GCTNAGC 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 1350
BspFNI CGCG 2 cut(s) 1406, 3622
BspHI TCATGA 1 cut(s) 1873
BspMAI CTGCAG 3 cut(s) 498, 3021, 3115
BspMI ACCTGC 3 cut(s) 487, 1305, 3641
BspPI GGATC 6 cut(s) 452, 1649, 1790, 2242, 3648, 4289
BspQI GCTCTTC 1 cut(s) 1440
BspT107I GGYRCC 2 cut(s) 1913, 4358
BspTI CTTAAG 3 cut(s) 1259, 2710, 4157
BsrDI GCAATG 2 cut(s) 701, 3326
BsrFI RCCGGY 2 cut(s) 1374, 2877
BsrI ACTGG 7 cut(s) 319, 334, 439, 1077, 2751, 3642, 3847
BssAI RCCGGY 2 cut(s) 1374, 2877
BssECI CCNNGG 5 cut(s) 1025, 1350, 1765, 1864, 3237
BssNAI GTATAC 1 cut(s) 1617
BssNI GRCGYC 1 cut(s) 7
BssSI CACGAG 1 cut(s) 3603
BssT1I CCWWGG 2 cut(s) 1350, 1765
Bst1107I GTATAC 1 cut(s) 1617
Bst2BI CACGAG 1 cut(s) 3603
Bst2UI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
Bst6I CTCTTC 5 cut(s) 512, 1440, 2442, 3792, 3867
BstACI GRCGYC 1 cut(s) 7
BstAFI CTTAAG 3 cut(s) 1259, 2710, 4157
BstAPI GCANNNNNTGC 2 cut(s) 250, 2495
BstDSI CCRYGG 1 cut(s) 1350
BstENI CCTNNNNNAGG 2 cut(s) 2372, 4389
BstFNI CGCG 2 cut(s) 1406, 3622
BstHHI GCGC 4 cut(s) 1091, 2958, 3622, 3635
BstMAI GTCTC 6 cut(s) 1961, 2547, 3606, 3732, 4310, 4348
BstMCI CGRYCG 1 cut(s) 3400
BstNI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
BstNSI RCATGY 9 cut(s) 96, 554, 576, 701, 761, 845, 1936, 2505, 4253
BstPAI GACNNNNGTC 1 cut(s) 2790
BstSCI CCNGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3237
BstSFI CTRYAG 6 cut(s) 494, 1092, 1265, 1648, 3017, 3111
BstSLI GKGCMC 3 cut(s) 322, 3238, 4361
BstUI CGCG 2 cut(s) 1406, 3622
BstV2I GAAGAC 3 cut(s) 336, 1491, 2723
BstX2I RGATCY 2 cut(s) 2247, 3640
BstXI CCANNNNNNTGG 3 cut(s) 2301, 2422, 2990
BstYI RGATCY 2 cut(s) 2247, 3640
BstZ17I GTATAC 1 cut(s) 1617
Bsu36I CCTNAGG 2 cut(s) 850, 3096
BsuI GTATCC 1 cut(s) 1936
BtgI CCRYGG 1 cut(s) 1350
BtrI CACGTC 1 cut(s) 925
BtsI GCAGTG 2 cut(s) 1827, 1949
BveI ACCTGC 3 cut(s) 487, 1305, 3641
CaiI CAGNNNCTG 3 cut(s) 3283, 3503, 3656
CciI TCATGA 1 cut(s) 1873
CfoI GCGC 4 cut(s) 1091, 2958, 3622, 3635
Cfr10I RCCGGY 2 cut(s) 1374, 2877
Cfr13I GGNCC 8 cut(s) 1022, 1107, 2399, 2875, 3301, 3678, 3787, 4138
CseI GACGC 3 cut(s) 168, 3587, 3695
Csp6I GTAC 4 cut(s) 1402, 1825, 1914, 2847
CspCI CAANNNNNGTGG 2 cut(s) 3425, 3460
CviQI GTAC 4 cut(s) 1402, 1825, 1914, 2847
DraI TTTAAA 2 cut(s) 210, 2568
DrdI GACNNNNNNGTC 1 cut(s) 1055
DseDI GACNNNNNNGTC 1 cut(s) 1055
EaeI YGGCCR 2 cut(s) 281, 2748
Eam1104I CTCTTC 5 cut(s) 512, 1440, 2442, 3792, 3867
EarI CTCTTC 5 cut(s) 512, 1440, 2442, 3792, 3867
Ecl136II GAGCTC 1 cut(s) 3542
Eco130I CCWWGG 2 cut(s) 1350, 1765
Eco24I GRGCYC 2 cut(s) 1574, 3544
Eco47I GGWCC 5 cut(s) 1022, 1107, 3301, 3787, 4138
Eco53kI GAGCTC 1 cut(s) 3542
Eco57I CTGAAG 8 cut(s) 32, 2948, 2952, 3480, 3555, 4036, 4302, 4410
Eco81I CCTNAGG 2 cut(s) 850, 3096
Eco88I CYCGRG 2 cut(s) 3354, 4028
EcoICRI GAGCTC 1 cut(s) 3542
EcoNI CCTNNNNNAGG 2 cut(s) 2372, 4389
EcoO109I RGGNCCY 4 cut(s) 1022, 1107, 2399, 3301
EcoRI GAATTC 1 cut(s) 2207
EcoRII CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3237
EcoT14I CCWWGG 2 cut(s) 1350, 1765
EcoT22I ATGCAT 7 cut(s) 98, 255, 645, 703, 1938, 2468, 3370
EcoT38I GRGCYC 2 cut(s) 1574, 3544
ErhI CCWWGG 2 cut(s) 1350, 1765
FaqI GGGAC 5 cut(s) 1008, 1043, 3931, 3954, 4151
FauI CCCGC 2 cut(s) 589, 1097
FauNDI CATATG 2 cut(s) 249, 2662
FbaI TGATCA 3 cut(s) 178, 913, 2668
FblI GTMKAC 3 cut(s) 772, 1616, 3218
FriOI GRGCYC 2 cut(s) 1574, 3544
FspAI RTGCGCAY 1 cut(s) 3634
FspBI CTAG 8 cut(s) 506, 897, 2057, 2204, 2622, 2853, 3926, 3986
FspI TGCGCA 1 cut(s) 3634
GlaI GCGC 4 cut(s) 1090, 2957, 3621, 3634
GsaI CCCAGC 3 cut(s) 1836, 3507, 4070
GsuI CTGGAG 9 cut(s) 1775, 1985, 2004, 2244, 2707, 3084, 3864, 4037, 4169
HapII CCGG 4 cut(s) 280, 1375, 2878, 4398
HgaI GACGC 3 cut(s) 168, 3587, 3695
HhaI GCGC 4 cut(s) 1091, 2958, 3622, 3635
Hin1I GRCGYC 1 cut(s) 7
Hin6I GCGC 4 cut(s) 1089, 2956, 3620, 3633
HinP1I GCGC 4 cut(s) 1089, 2956, 3620, 3633
HincII GTYRAC 4 cut(s) 2631, 3577, 4044, 4239
HindII GTYRAC 4 cut(s) 2631, 3577, 4044, 4239
HindIII AAGCTT 2 cut(s) 3344, 3492
HpaI GTTAAC 1 cut(s) 4044
HpaII CCGG 4 cut(s) 280, 1375, 2878, 4398
Hpy99I CGWCG 1 cut(s) 2531
HpyCH4IV ACGT 2 cut(s) 924, 4212
HpySE526I ACGT 2 cut(s) 924, 4212
Hsp92I GRCGYC 1 cut(s) 7
HspAI GCGC 4 cut(s) 1089, 2956, 3620, 3633
KflI GGGWCCC 1 cut(s) 1022
KpnI GGTACC 1 cut(s) 1917
KroI GCCGGC 1 cut(s) 1374
KroNI GCCGGC 1 cut(s) 1376
Ksp22I TGATCA 3 cut(s) 178, 913, 2668
KspAI GTTAAC 1 cut(s) 4044
LguI GCTCTTC 1 cut(s) 1440
MaeI CTAG 8 cut(s) 506, 897, 2057, 2204, 2622, 2853, 3926, 3986
MaeII ACGT 2 cut(s) 924, 4212
MaeIII GTNAC 9 cut(s) 216, 451, 1640, 2635, 3033, 3142, 3825, 3879, 4208
MfeI CAATTG 3 cut(s) 776, 2406, 3437
MflI RGATCY 2 cut(s) 2247, 3640
MhlI GDGCHC 7 cut(s) 322, 1574, 2526, 3198, 3238, 3544, 4361
MlsI TGGCCA 1 cut(s) 2750
MluNI TGGCCA 1 cut(s) 2750
MlyI GAGTC 2 cut(s) 3608, 4350
MmeI TCCRAC 6 cut(s) 285, 1818, 2282, 3589, 4090, 4394
Mox20I TGGCCA 1 cut(s) 2750
Mph1103I ATGCAT 7 cut(s) 98, 255, 645, 703, 1938, 2468, 3370
MroNI GCCGGC 1 cut(s) 1374
MroXI GAANNNNTTC 3 cut(s) 1158, 1385, 4380
MscI TGGCCA 1 cut(s) 2750
MslI CAYNNNNRTG 4 cut(s) 563, 2181, 2420, 2988
Msp20I TGGCCA 1 cut(s) 2750
MspA1I CMGCKG 5 cut(s) 184, 622, 887, 2762, 3280
MspCI CTTAAG 3 cut(s) 1259, 2710, 4157
MspI CCGG 4 cut(s) 280, 1375, 2878, 4398
MspR9I CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
MunI CAATTG 3 cut(s) 776, 2406, 3437
Mva1269I GAATGC 5 cut(s) 70, 253, 1475, 2018, 2338
MvaI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
MvnI CGCG 2 cut(s) 1406, 3622
NaeI GCCGGC 1 cut(s) 1376
NcoI CCATGG 1 cut(s) 1350
NdeI CATATG 2 cut(s) 249, 2662
NgoMIV GCCGGC 1 cut(s) 1374
NmeAIII GCCGAG 1 cut(s) 2940
NmuCI GTSAC 4 cut(s) 451, 1640, 2635, 4208
NsbI TGCGCA 1 cut(s) 3634
NsiI ATGCAT 7 cut(s) 98, 255, 645, 703, 1938, 2468, 3370
NspI RCATGY 9 cut(s) 96, 554, 576, 701, 761, 845, 1936, 2505, 4253
PaeI GCATGC 4 cut(s) 96, 761, 845, 2505
PagI TCATGA 1 cut(s) 1873
PciI ACATGT 1 cut(s) 572
PciSI GCTCTTC 1 cut(s) 1440
PcsI WCGNNNNNNNCGW 1 cut(s) 2170
PctI GAATGC 5 cut(s) 70, 253, 1475, 2018, 2338
PdiI GCCGGC 1 cut(s) 1376
PdmI GAANNNNTTC 3 cut(s) 1158, 1385, 4380
PflMI CCANNNNNTGG 2 cut(s) 329, 4147
PfoI TCCNGGA 1 cut(s) 30
PleI GAGTC 2 cut(s) 3607, 4349
PpsI GAGTC 2 cut(s) 3607, 4349
PpuMI RGGWCCY 3 cut(s) 1022, 1107, 3301
PscI ACATGT 1 cut(s) 572
PshAI GACNNNNGTC 1 cut(s) 2790
PshBI ATTAAT 1 cut(s) 102
Psp124BI GAGCTC 1 cut(s) 3544
Psp5II RGGWCCY 3 cut(s) 1022, 1107, 3301
Psp6I CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3237
PspFI CCCAGC 3 cut(s) 1832, 3503, 4066
PspGI CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3237
PspPI GGNCC 8 cut(s) 1022, 1107, 2399, 2875, 3301, 3678, 3787, 4138
PspPPI RGGWCCY 3 cut(s) 1022, 1107, 3301
PsrI GAACNNNNNNTAC 2 cut(s) 1248, 1280
PstI CTGCAG 3 cut(s) 498, 3021, 3115
PstNI CAGNNNCTG 3 cut(s) 3283, 3503, 3656
PsuI RGATCY 2 cut(s) 2247, 3640
PvuII CAGCTG 3 cut(s) 184, 887, 2762
RsaI GTAC 4 cut(s) 1403, 1826, 1915, 2848
RsaNI GTAC 4 cut(s) 1402, 1825, 1914, 2847
RseI CAYNNNNRTG 4 cut(s) 563, 2181, 2420, 2988
SacI GAGCTC 1 cut(s) 3544
SapI GCTCTTC 1 cut(s) 1440
Sau96I GGNCC 8 cut(s) 1022, 1107, 2399, 2875, 3301, 3678, 3787, 4138
ScaI AGTACT 1 cut(s) 2848
SchI GAGTC 2 cut(s) 3608, 4350
ScrFI CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3239
SduI GDGCHC 7 cut(s) 322, 1574, 2526, 3198, 3238, 3544, 4361
SfcI CTRYAG 6 cut(s) 494, 1092, 1265, 1648, 3017, 3111
SinI GGWCC 5 cut(s) 1022, 1107, 3301, 3787, 4138
SmiMI CAYNNNNRTG 4 cut(s) 563, 2181, 2420, 2988
SmlI CTYRAG 4 cut(s) 1259, 1271, 2710, 4157
SmoI CTYRAG 4 cut(s) 1259, 1271, 2710, 4157
SphI GCATGC 4 cut(s) 96, 761, 845, 2505
SspI AATATT 1 cut(s) 972
SspMI CTAG 8 cut(s) 506, 897, 2057, 2204, 2622, 2853, 3926, 3986
SstI GAGCTC 1 cut(s) 3544
StyD4I CCNGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3237
StyI CCWWGG 2 cut(s) 1350, 1765
TaiI ACGT 2 cut(s) 927, 4215
TaqI TCGA 6 cut(s) 1975, 2173, 2526, 3203, 3510, 4081
TaqII GACCGA 1 cut(s) 3386
TatI WGTACW 1 cut(s) 2846
TauI GCSGC 2 cut(s) 3283, 3757
TseFI GTSAC 4 cut(s) 451, 1640, 2635, 4208
Tsp45I GTSAC 4 cut(s) 451, 1640, 2635, 4208
TspGWI ACGGA 4 cut(s) 247, 569, 2815, 3844
Van91I CCANNNNNTGG 2 cut(s) 329, 4147
Vha464I CTTAAG 3 cut(s) 1259, 2710, 4157
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 5 cut(s) 1022, 1107, 3301, 3787, 4138
VspI ATTAAT 1 cut(s) 102
XagI CCTNNNNNAGG 2 cut(s) 2372, 4389
XapI RAATTY 5 cut(s) 385, 1165, 1904, 2052, 2207
XbaI TCTAGA 1 cut(s) 2203
XceI RCATGY 9 cut(s) 96, 554, 576, 701, 761, 845, 1936, 2505, 4253
XcmI CCANNNNNNNNNTGG 3 cut(s) 3701, 3873, 4012
XmiI GTMKAC 3 cut(s) 772, 1616, 3218
XmnI GAANNNNTTC 3 cut(s) 1158, 1385, 4380
XspI CTAG 8 cut(s) 506, 897, 2057, 2204, 2622, 2853, 3926, 3986
ZrmI AGTACT 1 cut(s) 2848
Zsp2I ATGCAT 7 cut(s) 98, 255, 645, 703, 1938, 2468, 3370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.