Rw1G030840

ARF guanine-nucleotide exchange factor GNOM-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001807
Physical Location & Seq
Reverse (-)
58832457 .. 58837790
5334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030840.1

Sequence Viewer

Length: 4131 bp
ATGAGGAGGAATGTAAGATGGGGAGGACGGTATGCATCGGATGATGATCAGATGGAACACTCTCTAATCCAGTCTTTGAAGGCTTTGCGCAAGAAAATTTTTTCGTGGCAGCATCAGTTGAATAGCATCAGTCCAGCTGTGTATCTCAAGCCATTTCTGGATGTGATTAGATCTGACGAAACTGGTGCACCAATCACTGGTGTTGCTCTGGCATCTGTGTACAATATCTTAACACTGGATGTGATTGATCAGAACTCTGTCAACGTTGAAGATGCTATGCACTTGTTAGTTGATGCTATCACTGGCTGCCGATTTGAGGTGACTGACCCTGCATCAGAAGAAGTGGTACTTATGAAGATACTACAGGTTCTTCTGGCTTGCATGAAGAGTAAAGCATCTTTTATGTTGAGTAATCAGCAGGTTTGCACTATAGTGAATACATGTTTCCGTATAGTTCATCAAGCAGGAACAAAAGGTGAGTTGTTGCAACGGATAGCTCGCCACACAATGCATGAGCTTGTTAGATGTATTTTCTCACACCTTCCAGATATCCGCAGCACCGAAAATGCATTGTCAAATGGAAGCAATTCTCTCAAACAAGAGACTTCAGGGCTCAATAATGAGCATGCACTTGAGAGTAGACAATTGGATAATGGCAGCATTAGTTCTGAGTATGACAGTCAACTATTATCCACAAATCCTGCTTTGCATGCTTCACCAGGCATTGTAGAATCTGTGATGGGTGAGAAGACAACCGGGGCTTCTGACAGGAAAGATGCTGTGCAAAATGACTTGAATCTCATGACTGAGCCTTTTGGGGTGCCCAGCATGGTGGAAATTTTTCAGTTTCTTTGTTCTTTGTTAAACGTTGTTGAGCATATAGGATCGGGTCCTAAATCTAATCCCATAGCATTTGATGAAGATGTGCCTCTTTTTGCCTTGACCCTGGTTAATTCGGCTATAGAGTTGGGTGGGTCCTCCATTCAGCATCACCCCAAGTTATTGAGTTTGGTTCGGGATGAATTGTTTCAAAATCTAATGCAATTTGGCCTGTCAACGAGCCCACTTATTCTTTCAATGGTATGCAGCATTGTTCTTAATTTGTATCACCATCTGCGCACTGAACTTAAACTACAGCTTGAGGCTTTCTTTTCCTGCGTGATACTGAGGCTTGCACAAAGTAGGTATGGAGCTTCTTATCAACAGCAGGAGGTGGCCATGGAAGCTCTAGTTGATTTCTGCAGGCAGAAAAATTTCATGGTGGAGATGTATGCTAATTTAGATTGTGACATAACTTGCAGTAATATCTTTGAAGAGCTTGCTAATCTGTTGTCAAAGAGTGCATTCCCAGTTAATAGCCCTTTGTCTTCCTTTCACATTCTTGCTTTGGATGGCCTTATTGCTGTTATTCAGGCAATGACAGAGAGGGTAGAGAATGGATCTTATAGTTTATCTCATACTCCAGTGACTCTTGAGGAGTATACTCCATTCTGGATGGTGAAGTGTGACAACTACAGTGATCCTAATCATTGGGTTCCATTTGTTCGCCAGACGAAGTACATAAAGCGAAGGTTGATAACTGGTGCCGATCATTTTAACCATGACCCAAAGAAAGGGCTGGAATTCCTCCAAGGAATTCATCTCTTGCCTGAGAAACTTGATCCTCAAAGTGTGGCATGCTTTTTCAGGTATACGGCTGGGTTAGACAAGAATCTTATTGGGGATTTCTTGGGAAATCATGATGACTTTTGCATTCAGGTTCTTCATAAATTTGCTGGTACCTTTGATTTCCAAGATATGAACTTGGATACTGCACTCCGCCTTTTTTTGGAAACTTTTAGATTGCCTGGTGAATCACAGAAAATACAGAGGGTGCTTGAAGCATTCTCAGAGAGATACTATGAGCAATCCCCACTAATTCTAGCTGACAAGGATGCTGCTCTTCTTTTATCTTATTCAATCATAATGCTCAATACAGATCAGCACAATGTTCAGGTGAAGAAGAAGATGACAGAGGAGGATTTCATTCGAAATAATCGGCACATCAATGGTGGCGAGGATCTTCCCCGAGAGTTCCTGTCAGAGCTTTACCACTCAATATGCAAGAATGAGATCCGCACTACTCCAGAACAAGGTGCTGGTTTTCCTGAAATGACTCCAAGCAGATGGATTGATTTGATGCACAAATCCAAGAAGAATGCTCCATTTATTGTATCTGATTCCAGAGCCTACCTTGATCAGGATATGTTCTCTATAGTGTCTGGACCCACAATTGCTGCTATATCTGTGGTATTTGATAATGCTGAACAGGAAGAGGTTTACCAAACATGTCTCGATGGCTTTTTAGCTGTTGCCAAGATTTCAGCATGCTATCATCTTGAAGATGTTCTGGATGATCTGGTTGTGTCTCTCTGTAAGTTCACAACCCTCTTAAATTCATCATTTCATGACGAACCTGAACTAGAATTTGGTGATGATATAAAAGCGAGAATGTCAACTGTGACAGTTTTCACAATTGCGAATCGGTATGGTGATTACATTCGTACAGGATGGAGAAATATTATTGATTGTATATTAAGATTACACAAGCTTGGTCTTCTTCCGGCTCGTGTGGCACAAGATGCAGCTGATGAGTCGGAGTTTCCCTCTGAGACAGATCATGGAAAACCTATCACAAATTCCCAATCTAAAATTCATACGCCTTCCGTTAGTACTCCTAAGAAATCCTCTGGATTCATGGGTCGGTTTAGTCAGCTCTTATCTCTTGATACAGAGGAGCCGAAACCACAGCCAACTGAAGAAGAACTTGCTACTCGTCAGCGCACCGTACAGACAGTTGAAGAGTGCCACATTGACAGCATATTTACCGAAAGCAAGTTTTTGCAGGCCGAATCCCTCTTACAGCTTTCACAAGCACTGATCTGGGCTGCAGGGCGACTTCAGAAAGGGAATAACTCTGCTGAGGATGAAGATACTGTTGTCTTTTGCTTGGAGTTGCTGATTGCTATTACCCTGAATAATAGGGATAGGATTATGCTTCTTTGGCAGGGTGTGTATGATTTCATATCCAACATTGTGCAGTCAACTGTCATGCCTTGTGCCTTGGTAGAAAAGGCTGTCTTTGGACTTCTTCGGATTTGTCAGCGGCTGCTTCCCTATAAGGAAAACCTTTCTGATGATCTTTTGAGGTCACTGCAGCTTGTCTTGAAGCTTGATGCTCGGGTTGCCGATGCATACTGTGAGCAAATTACACTGGAAGTCAGTAGGCTTGTAAAGGCAAATGCCTCTCACATCAGATCTCAATTGGGGTGGCGCACAATTACGTCCCTACTCTCAATCACTGCTCGGCACCCGGATGCTTCTGAGGCTGGTTTTGACGCACTGTTCTTCATTATGTCAGGTGGAACTCACTTGTTGCCAGCAAATTATGTTCTCTGTGTCGATGCGTCACGGCAGTTTGCTGAGTCTCGTGTTGGGCAGGCAGATCGGTCTGTCTGTGCACTGGATCTTATGGCAGGTTCTGTTGATTGTTTAGCAAGATGGGCCTGTGAGGCTAAGCTATCTATGAATGAGGAGGATTCTGTGAAAATGTCTCAGGATATTGGGGAAATGTGGTTGAGGCTTGTTCAGGGATTGAGAAAAGTTTGTTTAGACCAAAGAGAAGTGGTTAGGAACCATGCTTTGTCGATGTTACGGAACTGCTTGACAGGAGTTGATGGGATCCCTCTTCCATATGGTCTCTGGTTGCAGTGCTTTGATCTTGTGATTTTCACAGTGCTTGATGATTTGCTTGAAATTGCACAGGTTCACTCCCAAAAGGAGTACCGCAACATGGAAGGCACTCTTATCCTGGCCATGAAGCTCTTGTCCAAAGTTCGAATGGAAAACTATATGAAGGTGAAAGTTGGAGGGAGGAAAAGTGAGAAGCTTCAGGACGAAGTACCTGAGGGAGTGCTTGTTCAAAAGAGTGGTTCAGGTGAGGATGGCTTAATTGCTCCATCTTTGCAAGCTGAGGTGCTTGCTCAAATTCTAGTGCAGTCACATACTGAGCAGGGTGAAACAGGAGTATCTGCTGAAAAGGGTTCTTCAACTGAAACAATACCCTCTGGAGGTTCTGGCTGTGGTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Sec7 Sec7_N

Protein Analysis

1376

Amino Acids

153.85

Weight (kDa)

5.26

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 276 - 436 3.9e-40 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 522 - 705 9.6e-72 Sec7 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 89, 1118
Acc36I ACCTGC 2 cut(s) 409, 3506
Acc65I GGTACC 1 cut(s) 1778
AccB1I GGYRCC 4 cut(s) 820, 1583, 1778, 3348
AccB7I CCANNNNNTGG 1 cut(s) 197
AccI GTMKAC 3 cut(s) 640, 1481, 1691
AciI CCGC 5 cut(s) 553, 1819, 2116, 3145, 3826
AclI AACGTT 2 cut(s) 264, 867
AclWI GGATC 9 cut(s) 892, 1447, 1514, 1655, 2067, 2107, 3513, 3715, 3728
AcoI YGGCCR 2 cut(s) 1215, 3852
AcuI CTGAAG 4 cut(s) 591, 2817, 2924, 3914
AfaI GTAC 9 cut(s) 221, 348, 1559, 1780, 2545, 2713, 2829, 3824, 3942
AfiI CCNNNNNNNGG 8 cut(s) 197, 316, 1613, 1828, 2132, 2239, 3832, 4109
AflIII ACRYGT 2 cut(s) 440, 2327
AjnI CCWGG 4 cut(s) 718, 945, 1846, 3849
AjuI GAANNNNNNNTTGG 4 cut(s) 2451, 2483, 2676, 2708
AleI CACNNNNGTG 1 cut(s) 431
AloI GAACNNNNNNTCC 2 cut(s) 3942, 3974
Alw21I GWGCWC 2 cut(s) 190, 3502
Alw26I GTCTC 7 cut(s) 596, 2336, 2412, 2645, 3471, 3597, 3743
Alw44I GTGCAC 2 cut(s) 186, 3498
AlwI GGATC 9 cut(s) 892, 1447, 1514, 1655, 2067, 2107, 3513, 3715, 3728
AlwNI CAGNNNCTG 2 cut(s) 3148, 3521
Ama87I CYCGRG 2 cut(s) 2067, 3219
AoxI GGCC 6 cut(s) 1048, 1215, 1393, 2886, 3543, 3852
ApaLI GTGCAC 2 cut(s) 186, 3498
Asp700I GAANNNNTTC 5 cut(s) 586, 840, 1026, 1253, 2385
Asp718I GGTACC 1 cut(s) 1778
AspLEI GCGC 4 cut(s) 90, 1119, 2823, 3315
AspS9I GGNCC 4 cut(s) 890, 975, 2264, 3543
AsuC2I CCSGG 2 cut(s) 757, 3353
AsuII TTCGAA 2 cut(s) 2029, 3877
AvaI CYCGRG 2 cut(s) 2067, 3219
AvaII GGWCC 3 cut(s) 890, 975, 2264
AxyI CCTNAGG 1 cut(s) 3945
BaeGI GKGCMC 3 cut(s) 190, 825, 3502
BaeI ACNNNNGTAYC 4 cut(s) 2964, 2997, 4050, 4083
BalI TGGCCA 2 cut(s) 1217, 3854
BamHI GGATCC 1 cut(s) 3720
BanI GGYRCC 4 cut(s) 820, 1583, 1778, 3348
BanII GRGCYC 2 cut(s) 615, 1064
BarI GAAGNNNNNNTAC 2 cut(s) 330, 362
BauI CACGAG 2 cut(s) 2607, 3468
BbsI GAAGAC 3 cut(s) 755, 1359, 2588
Bbv12I GWGCWC 2 cut(s) 190, 3502
BbvCI CCTCAGC 2 cut(s) 2961, 4011
BceAI ACGGC 2 cut(s) 1710, 3467
BcgI CGANNNNNNTGC 4 cut(s) 167, 201, 3467, 3501
BciT130I CCWGG 4 cut(s) 720, 947, 1848, 3851
BciVI GTATCC 1 cut(s) 1801
BclI TGATCA 3 cut(s) 46, 247, 2236
BcnI CCSGG 2 cut(s) 757, 3353
BcoDI GTCTC 7 cut(s) 596, 2336, 2412, 2645, 3471, 3597, 3743
BfaI CTAG 4 cut(s) 1229, 1922, 2462, 4031
BfmI CTRYAG 9 cut(s) 362, 429, 960, 1133, 1240, 1513, 2253, 2928, 3194
BfuAI ACCTGC 2 cut(s) 409, 3506
BfuI GTATCC 1 cut(s) 1801
BglI GCCNNNNNGGC 1 cut(s) 3551
BglII AGATCT 2 cut(s) 170, 3296
BlpI GCTNAGC 1 cut(s) 3555
BmcAI AGTACT 1 cut(s) 2713
Bme1390I CCNGG 6 cut(s) 720, 757, 947, 1848, 3353, 3851
Bme18I GGWCC 3 cut(s) 890, 975, 2264
BmeT110I CYCGRG 2 cut(s) 2067, 3219
BmgT120I GGNCC 4 cut(s) 890, 975, 2264, 3543
BmrFI CCNGG 6 cut(s) 720, 757, 947, 1848, 3353, 3851
BmrI ACTGGG 1 cut(s) 1343
BmuI ACTGGG 1 cut(s) 1343
BpiI GAAGAC 3 cut(s) 755, 1359, 2588
BplI GAGNNNNNCTC 2 cut(s) 2630, 2662
BpmI CTGGAG 3 cut(s) 1446, 2109, 4128
Bpu10I CCTNAGC 2 cut(s) 2961, 4011
Bpu1102I GCTNAGC 1 cut(s) 3555
Bpu14I TTCGAA 2 cut(s) 2029, 3877
BpuEI CTTGAG 4 cut(s) 131, 653, 1160, 1493
BpuMI CCSGG 2 cut(s) 757, 3353
BsaBI GATNNNNATC 2 cut(s) 45, 1524
BsaI GGTCTC 1 cut(s) 3743
BsaJI CCNNGG 5 cut(s) 756, 945, 1218, 1630, 3102
BsaXI ACNNNNNCTCC 4 cut(s) 3904, 3934, 3942, 3972
Bsc4I CCNNNNNNNGG 8 cut(s) 197, 316, 1613, 1828, 2132, 2239, 3832, 4109
Bse21I CCTNAGG 1 cut(s) 3945
Bse3DI GCAATG 1 cut(s) 1422
Bse8I GATNNNNATC 2 cut(s) 45, 1524
BseBI CCWGG 4 cut(s) 720, 947, 1848, 3851
BseDI CCNNGG 5 cut(s) 756, 945, 1218, 1630, 3102
BseJI GATNNNNATC 2 cut(s) 45, 1524
BseLI CCNNNNNNNGG 8 cut(s) 197, 316, 1613, 1828, 2132, 2239, 3832, 4109
BseMI GCAATG 1 cut(s) 1422
BseRI GAGGAG 5 cut(s) 19, 1490, 2030, 2789, 3587
BseSI GKGCMC 3 cut(s) 190, 825, 3502
BseYI CCCAGC 2 cut(s) 824, 1697
BsgI GTGCAG 3 cut(s) 1797, 3098, 4055
BshFI GGCC 6 cut(s) 1050, 1217, 1395, 2888, 3545, 3854
BshNI GGYRCC 4 cut(s) 820, 1583, 1778, 3348
BsiHKAI GWGCWC 2 cut(s) 190, 3502
BsiHKCI CYCGRG 2 cut(s) 2067, 3219
BsiSI CCGG 3 cut(s) 756, 2603, 3353
BslFI GGGAC 1 cut(s) 3310
BslI CCNNNNNNNGG 8 cut(s) 197, 316, 1613, 1828, 2132, 2239, 3832, 4109
BsmAI GTCTC 7 cut(s) 596, 2336, 2412, 2645, 3471, 3597, 3743
BsmFI GGGAC 1 cut(s) 3310
BsmI GAATGC 4 cut(s) 1343, 1752, 1883, 2203
BsnI GGCC 6 cut(s) 1050, 1217, 1395, 2888, 3545, 3854
Bso31I GGTCTC 1 cut(s) 3743
BsoBI CYCGRG 2 cut(s) 2067, 3219
Bsp119I TTCGAA 2 cut(s) 2029, 3877
Bsp1286I GDGCHC 5 cut(s) 190, 615, 825, 1064, 3502
Bsp1407I TGTACA 1 cut(s) 219
Bsp1720I GCTNAGC 1 cut(s) 3555
Bsp19I CCATGG 1 cut(s) 1218
BspACI CCGC 5 cut(s) 553, 1819, 2116, 3145, 3826
BspANI GGCC 6 cut(s) 1050, 1217, 1395, 2888, 3545, 3854
BspHI TCATGA 3 cut(s) 801, 1738, 2446
BspMAI CTGCAG 3 cut(s) 1244, 2932, 3198
BspMI ACCTGC 2 cut(s) 409, 3506
BspPI GGATC 9 cut(s) 892, 1447, 1514, 1655, 2067, 2107, 3513, 3715, 3728
BspQI GCTCTTC 2 cut(s) 1308, 1947
BspT104I TTCGAA 2 cut(s) 2029, 3877
BspT107I GGYRCC 4 cut(s) 820, 1583, 1778, 3348
BspTNI GGTCTC 1 cut(s) 3743
BsrDI GCAATG 1 cut(s) 1422
BsrGI TGTACA 1 cut(s) 219
BssECI CCNNGG 5 cut(s) 756, 945, 1218, 1630, 3102
BssNAI GTATAC 2 cut(s) 1482, 1692
BssSI CACGAG 2 cut(s) 2607, 3468
BssT1I CCWWGG 3 cut(s) 1218, 1630, 3102
Bst1107I GTATAC 2 cut(s) 1482, 1692
Bst2BI CACGAG 2 cut(s) 2607, 3468
Bst2UI CCWGG 4 cut(s) 720, 947, 1848, 3851
Bst6I CTCTTC 6 cut(s) 380, 1308, 1947, 2307, 2835, 3732
BstAPI GCANNNNNTGC 1 cut(s) 2621
BstAUI TGTACA 1 cut(s) 219
BstBI TTCGAA 2 cut(s) 2029, 3877
BstDSI CCRYGG 1 cut(s) 1218
BstENI CCTNNNNNAGG 1 cut(s) 2237
BstHHI GCGC 4 cut(s) 90, 1119, 2823, 3315
BstMAI GTCTC 7 cut(s) 596, 2336, 2412, 2645, 3471, 3597, 3743
BstMWI GCNNNNNNNGC 9 cut(s) 710, 1223, 2612, 2621, 3043, 3224, 3365, 3542, 3551
BstNI CCWGG 4 cut(s) 720, 947, 1848, 3851
BstNSI RCATGY 6 cut(s) 444, 629, 713, 1680, 2331, 2370
BstSCI CCNGG 6 cut(s) 718, 755, 945, 1846, 3351, 3849
BstSFI CTRYAG 9 cut(s) 362, 429, 960, 1133, 1240, 1513, 2253, 2928, 3194
BstSLI GKGCMC 3 cut(s) 190, 825, 3502
BstV2I GAAGAC 3 cut(s) 755, 1359, 2588
BstX2I RGATCY 7 cut(s) 170, 1439, 2059, 2112, 3296, 3505, 3720
BstXI CCANNNNNNTGG 2 cut(s) 832, 2166
BstYI RGATCY 7 cut(s) 170, 1439, 2059, 2112, 3296, 3505, 3720
BstZ17I GTATAC 2 cut(s) 1482, 1692
Bsu36I CCTNAGG 1 cut(s) 3945
BsuI GTATCC 1 cut(s) 1801
BsuRI GGCC 6 cut(s) 1050, 1217, 1395, 2888, 3545, 3854
BtgI CCRYGG 1 cut(s) 1218
BtsI GCAGTG 3 cut(s) 3191, 3339, 3755
BveI ACCTGC 2 cut(s) 409, 3506
CaiI CAGNNNCTG 2 cut(s) 3148, 3521
CciI TCATGA 3 cut(s) 801, 1738, 2446
CfoI GCGC 4 cut(s) 90, 1119, 2823, 3315
Cfr13I GGNCC 4 cut(s) 890, 975, 2264, 3543
CseI GACGC 2 cut(s) 3386, 3435
Csp6I GTAC 9 cut(s) 220, 347, 1558, 1779, 2544, 2712, 2828, 3823, 3941
CspCI CAANNNNNGTGG 2 cut(s) 3290, 3325
CviQI GTAC 9 cut(s) 220, 347, 1558, 1779, 2544, 2712, 2828, 3823, 3941
EaeI YGGCCR 2 cut(s) 1215, 3852
Eam1104I CTCTTC 6 cut(s) 380, 1308, 1947, 2307, 2835, 3732
EarI CTCTTC 6 cut(s) 380, 1308, 1947, 2307, 2835, 3732
EciI GGCGGA 1 cut(s) 1808
Eco130I CCWWGG 3 cut(s) 1218, 1630, 3102
Eco24I GRGCYC 2 cut(s) 615, 1064
Eco31I GGTCTC 1 cut(s) 3743
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 3 cut(s) 890, 975, 2264
Eco57I CTGAAG 4 cut(s) 591, 2817, 2924, 3914
Eco81I CCTNAGG 1 cut(s) 3945
Eco88I CYCGRG 2 cut(s) 2067, 3219
EcoNI CCTNNNNNAGG 1 cut(s) 2237
EcoO109I RGGNCCY 2 cut(s) 890, 975
EcoRI GAATTC 2 cut(s) 1622, 1635
EcoRII CCWGG 4 cut(s) 718, 945, 1846, 3849
EcoRV GATATC 1 cut(s) 550
EcoT14I CCWWGG 3 cut(s) 1218, 1630, 3102
EcoT22I ATGCAT 4 cut(s) 37, 513, 571, 3235
EcoT38I GRGCYC 2 cut(s) 615, 1064
ErhI CCWWGG 3 cut(s) 1218, 1630, 3102
FalI AAGNNNNNCTT 8 cut(s) 333, 365, 2790, 2822, 3104, 3136, 3828, 3860
FaqI GGGAC 1 cut(s) 3310
FauNDI CATATG 1 cut(s) 3733
FbaI TGATCA 3 cut(s) 46, 247, 2236
FblI GTMKAC 3 cut(s) 640, 1481, 1691
FriOI GRGCYC 2 cut(s) 615, 1064
FspBI CTAG 4 cut(s) 1229, 1922, 2462, 4031
FspI TGCGCA 2 cut(s) 89, 1118
GlaI GCGC 4 cut(s) 89, 1118, 2822, 3314
GsaI CCCAGC 2 cut(s) 828, 1701
GsuI CTGGAG 3 cut(s) 1446, 2109, 4128
HaeIII GGCC 6 cut(s) 1050, 1217, 1395, 2888, 3545, 3854
HapII CCGG 3 cut(s) 756, 2603, 3353
HgaI GACGC 2 cut(s) 3386, 3435
HhaI GCGC 4 cut(s) 90, 1119, 2823, 3315
Hin6I GCGC 4 cut(s) 88, 1117, 2821, 3313
HinP1I GCGC 4 cut(s) 88, 1117, 2821, 3313
HincII GTYRAC 5 cut(s) 262, 683, 1056, 2496, 3084
HindII GTYRAC 5 cut(s) 262, 683, 1056, 2496, 3084
HindIII AAGCTT 3 cut(s) 2588, 3209, 3926
HpaII CCGG 3 cut(s) 756, 2603, 3353
HpyAV CCTTC 6 cut(s) 73, 551, 1563, 2712, 3830, 3889
HpyCH4IV ACGT 3 cut(s) 264, 867, 3323
HpyF10VI GCNNNNNNNGC 9 cut(s) 710, 1223, 2612, 2621, 3043, 3224, 3365, 3542, 3551
HpySE526I ACGT 3 cut(s) 264, 867, 3323
HspAI GCGC 4 cut(s) 88, 1117, 2821, 3313
KpnI GGTACC 1 cut(s) 1782
Ksp22I TGATCA 3 cut(s) 46, 247, 2236
LguI GCTCTTC 2 cut(s) 1308, 1947
LmnI GCTCC 4 cut(s) 1190, 2206, 2776, 4000
MaeI CTAG 4 cut(s) 1229, 1922, 2462, 4031
MaeII ACGT 3 cut(s) 264, 867, 3323
MaeIII GTNAC 9 cut(s) 319, 1286, 1465, 1505, 2500, 3189, 3447, 3690, 4038
MfeI CAATTG 4 cut(s) 644, 2271, 2514, 3302
MflI RGATCY 7 cut(s) 170, 1439, 2059, 2112, 3296, 3505, 3720
MhlI GDGCHC 5 cut(s) 190, 615, 825, 1064, 3502
MlsI TGGCCA 2 cut(s) 1217, 3854
MluNI TGGCCA 2 cut(s) 1217, 3854
MlyI GAGTC 4 cut(s) 1462, 2149, 2642, 3473
MmeI TCCRAC 3 cut(s) 2616, 3093, 3886
Mox20I TGGCCA 2 cut(s) 1217, 3854
Mph1103I ATGCAT 4 cut(s) 37, 513, 571, 3235
MroXI GAANNNNTTC 5 cut(s) 586, 840, 1026, 1253, 2385
MscI TGGCCA 2 cut(s) 1217, 3854
MslI CAYNNNNRTG 3 cut(s) 431, 2046, 3354
Msp20I TGGCCA 2 cut(s) 1217, 3854
MspA1I CMGCKG 3 cut(s) 137, 2627, 3145
MspI CCGG 3 cut(s) 756, 2603, 3353
MspR9I CCNGG 6 cut(s) 720, 757, 947, 1848, 3353, 3851
MunI CAATTG 4 cut(s) 644, 2271, 2514, 3302
Mva1269I GAATGC 4 cut(s) 1343, 1752, 1883, 2203
MvaI CCWGG 4 cut(s) 720, 947, 1848, 3851
MwoI GCNNNNNNNGC 9 cut(s) 710, 1223, 2612, 2621, 3043, 3224, 3365, 3542, 3551
NciI CCSGG 2 cut(s) 757, 3353
NcoI CCATGG 1 cut(s) 1218
NdeI CATATG 1 cut(s) 3733
NmeAIII GCCGAG 1 cut(s) 3325
NmuCI GTSAC 8 cut(s) 319, 1286, 1465, 1505, 2500, 3189, 3447, 4038
NsbI TGCGCA 2 cut(s) 89, 1118
NsiI ATGCAT 4 cut(s) 37, 513, 571, 3235
NspI RCATGY 6 cut(s) 444, 629, 713, 1680, 2331, 2370
NspV TTCGAA 2 cut(s) 2029, 3877
OliI CACNNNNGTG 1 cut(s) 431
PaeI GCATGC 4 cut(s) 629, 713, 1680, 2370
PagI TCATGA 3 cut(s) 801, 1738, 2446
PciI ACATGT 2 cut(s) 440, 2327
PciSI GCTCTTC 2 cut(s) 1308, 1947
PctI GAATGC 4 cut(s) 1343, 1752, 1883, 2203
PdmI GAANNNNTTC 5 cut(s) 586, 840, 1026, 1253, 2385
PfeI GAWTC 9 cut(s) 731, 796, 1711, 1853, 2219, 2521, 2733, 2891, 3578
PflMI CCANNNNNTGG 1 cut(s) 197
PleI GAGTC 4 cut(s) 1462, 2149, 2641, 3472
PpsI GAGTC 4 cut(s) 1462, 2149, 2641, 3472
PpuMI RGGWCCY 2 cut(s) 890, 975
PscI ACATGT 2 cut(s) 440, 2327
Psp1406I AACGTT 2 cut(s) 264, 867
Psp5II RGGWCCY 2 cut(s) 890, 975
Psp6I CCWGG 4 cut(s) 718, 945, 1846, 3849
PspFI CCCAGC 2 cut(s) 824, 1697
PspGI CCWGG 4 cut(s) 718, 945, 1846, 3849
PspPI GGNCC 4 cut(s) 890, 975, 2264, 3543
PspPPI RGGWCCY 2 cut(s) 890, 975
PsrI GAACNNNNNNTAC 2 cut(s) 1116, 1148
PstI CTGCAG 3 cut(s) 1244, 2932, 3198
PstNI CAGNNNCTG 2 cut(s) 3148, 3521
PsuI RGATCY 7 cut(s) 170, 1439, 2059, 2112, 3296, 3505, 3720
PvuII CAGCTG 2 cut(s) 137, 2627
RsaI GTAC 9 cut(s) 221, 348, 1559, 1780, 2545, 2713, 2829, 3824, 3942
RsaNI GTAC 9 cut(s) 220, 347, 1558, 1779, 2544, 2712, 2828, 3823, 3941
RseI CAYNNNNRTG 3 cut(s) 431, 2046, 3354
SapI GCTCTTC 2 cut(s) 1308, 1947
Sau96I GGNCC 4 cut(s) 890, 975, 2264, 3543
ScaI AGTACT 1 cut(s) 2713
SchI GAGTC 4 cut(s) 1462, 2149, 2642, 3473
ScrFI CCNGG 6 cut(s) 720, 757, 947, 1848, 3353, 3851
SduI GDGCHC 5 cut(s) 190, 615, 825, 1064, 3502
SfcI CTRYAG 9 cut(s) 362, 429, 960, 1133, 1240, 1513, 2253, 2928, 3194
SfuI TTCGAA 2 cut(s) 2029, 3877
SinI GGWCC 3 cut(s) 890, 975, 2264
SmiMI CAYNNNNRTG 3 cut(s) 431, 2046, 3354
SmlI CTYRAG 4 cut(s) 146, 632, 1139, 1472
SmoI CTYRAG 4 cut(s) 146, 632, 1139, 1472
SphI GCATGC 4 cut(s) 629, 713, 1680, 2370
SsiI CCGC 5 cut(s) 553, 1819, 2116, 3145, 3826
SspI AATATT 1 cut(s) 2560
SspMI CTAG 4 cut(s) 1229, 1922, 2462, 4031
StyD4I CCNGG 6 cut(s) 718, 755, 945, 1846, 3351, 3849
StyI CCWWGG 3 cut(s) 1218, 1630, 3102
TaiI ACGT 3 cut(s) 267, 870, 3326
TaqI TCGA 5 cut(s) 2029, 2334, 3441, 3686, 3877
TaqII GACCGA 1 cut(s) 3477
TatI WGTACW 3 cut(s) 219, 1557, 2711
TauI GCSGC 1 cut(s) 3148
TfiI GAWTC 9 cut(s) 731, 796, 1711, 1853, 2219, 2521, 2733, 2891, 3578
TseFI GTSAC 8 cut(s) 319, 1286, 1465, 1505, 2500, 3189, 3447, 4038
Tsp45I GTSAC 8 cut(s) 319, 1286, 1465, 1505, 2500, 3189, 3447, 4038
TspGWI ACGGA 4 cut(s) 437, 505, 2695, 3709
Van91I CCANNNNNTGG 1 cut(s) 197
VneI GTGCAC 2 cut(s) 186, 3498
VpaK11BI GGWCC 3 cut(s) 890, 975, 2264
XagI CCTNNNNNAGG 1 cut(s) 2237
XceI RCATGY 6 cut(s) 444, 629, 713, 1680, 2331, 2370
XcmI CCANNNNNNNNNTGG 2 cut(s) 3738, 3877
XmiI GTMKAC 3 cut(s) 640, 1481, 1691
XmnI GAANNNNTTC 5 cut(s) 586, 840, 1026, 1253, 2385
XspI CTAG 4 cut(s) 1229, 1922, 2462, 4031
ZrmI AGTACT 1 cut(s) 2713
Zsp2I ATGCAT 4 cut(s) 37, 513, 571, 3235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.