pycom01g15930

ARF guanine-nucleotide exchange factor GNOM-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001807
Physical Location & Seq
Reverse (-)
15659775 .. 15662977
3203 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15930.2

Sequence Viewer

Length: 3057 bp
ATGGGGCGTCTGAAGCTGCAAAGTGGAATCCAGGCAATTGGGGAAGAACCTGGGGAATGTGATGCAACATATTCTAATCATGCTACTTTAGCATGCATCATTAATTCAGAAATAGGCACTGTATTAGCGGTTATGAGGAGGAATGTGAGATGGGGAGGACGCTATATGTCTGGCGATGATCAGCTGGAACACCCTCTAATCCAGTCTTTGAAGGCACTGCGCAAGCAAATCTTTTCGTGGAAGCACCACATGCATTCCGTCAATCCTTCTGATTATCTCCAGCCATTTCTGGATGTGATTAGATCGGATGAAACTGGTGCACCAATTACTGGTGTGGCGTTGTCTTCTGTTTACAATATCTTAACACTTGATGTAATTGATCAAAATTCCTTAAATGTTGAAGATGCCATGCACTTATTAGTTGATGCAATCACTGGCTGCCGATTTGAGGTGATTGATCCTGCATCAGAAGAAGTGGTATTGATGAAGATACTGCAGGTTCTTCTAGCTTGCATGAAGAGTAAAGCATCTATTATACTGACCAATCAGCATGTTTGCACTATAGTGAACACATGTTTCCGTATTGTCCATCAAGCGGGAACAAAAGGTGAGTTGTTGCAGCGTATAGCTCGCCACACAATGCATGAACTTGTTAGATGTATTTTCTCACATCTTCCAGATGTTCACCCTGCTGAACGTGCATTGCCAAATGGAAGCAATACCATCAATGGAGAGATTGCAGGGAAAAATAATGAGCATGTTTCTGGAAGTAGACAATTGGAGAATGGCAACATGATTTCTGAGTTTGATAATCAGCTATTATCTACAAATCCTGCTTTGAACGCTTCCTCAGGTCTTGTAGAATCTGGGATGGATGAAAAGACAACTGGTGCTTCTAGCGGGAAGGAGGCTGGTCAATATGATGTGCAACTCATGGCTGAGCCATTTGGGGTTCCCTGCATGGTGGAAATATTTACCTTCCTGTGCTCTTTGTTAAATGTGGTTGAGCATATAGGAATGGGTCCCAGGTCTAATACCATAGCTTTTGATGAAGATGTGCCCCTTTTTGCATTGGGTTTGGTTAATTGTGCTATAGAGTTAGGTGGGTCCTCCATTCGGCACCACCCCAAGTTGTTAAGTTTGGTTCAGGATGAATTGTTTCAAAATCTGATGCAGTTTGGCCTGTCAACAAGTCCACTTATTCTTTCAATGGTATGCAGCATTGTCCTCAATCTGTATCACCATCTCCGCACTGAACTTAAGCTACAGCTCGAGGCTTTCTTTTCATGTGTGATATTGAGGCTTGTGCAAAGCAGGTATGGGGCTTCTTATCAGCAGCAGGAGGTTGCCATGGAGGCTGTTGTGGACTTCTGCCGGCAGAAAACTTTCATGGTGGAGATGTACGCGAACTTAGATTGCGACATAACATGCATTAATGTCTTTGAAGAGCTTGCTAATCTGTTGTCAAAGAGTGCATTCCCTGTTAGTTTGCCTTTGTCTTCAATTCACATTCTTGCTTTGGATGGTCTTATTGCTATTATTCAGGGAATGGCAGAGAGGGCAGGGAATGGCTCAGTTAGTGCTGTTCAGGCTCCCCCCACGAATCTTGATGAGTATACTCCATTCTGGCTGATGAAATGTGACAACTATAGTGATCCGAATCATTGGGTTCCATTTGTTCGCCGAAGGAAGCACATAAAGAGAAGGCTGATGATTGGAGCCGATCATTTTAACCATGACCCAAAGAAAGGGCTGGAGTTCCTCCAAGGAACCCATCTCTTGCCTGACAAACATGATCCTGAAAGTGTGGCCTGTTTTTTCAGGTACACTGCTGGGTTGGACAAGAATCTTGTTGGGGATTTCCTGGGAAACCATGATGAGTTTTGCATTCAGGTTCTTAATAAATTCGCCGGTACCTTTGATTTTCAAGACATGCATTTGGATACTGCACTGCGACTGTTTCTGGAGACGTTTCGATTGCCTGGAGAATCACAAAAGATACAAAGGGTGCTTGAAGCATTCTCAGAGAGATATTATGAGCAATCACCACAAATTCTTGCTAACAAGGATGCTGCTCTCCTGTTATCATATTCAATCATAATGCTCAACACAGACCAGCACAATGTTCAGGTGAAGAAGAAGATGACAGAGGAGGATTTCATTCGGAATAATAGACACATCAATGGAGGTGATGATTTACCTCGAGAATTCCTTTCAGAGCTTTACCATTCAATATGTAAGGATGAGATCCGCACAACTCCAGAACAAGGTGCTGGTTATCCTGAAATGAACCCAAGCCGTTGGATTGATTTAATACACAAATCGAAGAAGAGTGCTCCATTCATTGTATCAGATACCAGAGCCTACCTTGACCAGGACATGTTTGCTATAATGTCAGGCCCCACAATTGCTGCCATTTCTGTGGTATTCGATCATGCAGAACATGAAGAGATTTACCAAACATGCATTGATGGATTTTTATCTGTTGCAAAGATTGCAGCGTGCTACCATCTTGAAGATGTGCTCGACGATCTGGTTGTGTCTCTTTGTAAGTTTACAACCCTTTTAAACCCATCATCTTATGAGGAACCTGTTATAGCCTTTGGTGATGACCCAAAAGCTAGAATGTCAACTGTGACAGTTTTCACGTTTGCCAATACATATGGTGATTACATTCGCACAGGCTGGAGAAATGTTCTCGACTGTATCTTAAGATTACACAAACTTGGTCTTCTGTCGGCATTTGTGGCCAGTGAAGCAGCTGGTGATTCAGAGTCTTCTGCTGACACAGGTCATGGCAAGCCCATAACTAATTCTCTATCATCTGTCCCTATGCCCCCTGTGAGTACTCCTAGGAAATCCTCCGGATTTATGGGCCGGTTTAGTCAGCTCTTATCTCTTGACACAGAGGAGCCGAGATCGAACAGCCTACTGAAGAAGAACTTGCTGCTCATCAGCGCACCCTTCAGACAATTCAAAAGTGCCACATTGATGGCATATTTTCAGAGAGCAAGTTTCTGCAGGCTGAATCCCTGTTACAGCTTGCACAGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Sec7 Sec7_N

Protein Analysis

1019

Amino Acids

113.66

Weight (kDa)

5.74

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 487, 1305
Acc65I GGTACC 1 cut(s) 1913
AccB1I GGYRCC 2 cut(s) 1119, 1913
AccB7I CCANNNNNTGG 1 cut(s) 329
AccI GTMKAC 2 cut(s) 772, 1616
AccII CGCG 1 cut(s) 1406
AccIII TCCGGA 1 cut(s) 2864
AciI CCGC 5 cut(s) 128, 596, 900, 1249, 2251
AclWI GGATC 4 cut(s) 452, 1649, 1790, 2242
AcoI YGGCCR 1 cut(s) 2748
AcsI RAATTY 4 cut(s) 385, 1904, 2052, 2207
AcuI CTGAAG 3 cut(s) 32, 2950, 2954
AcyI GRCGYC 1 cut(s) 7
AfaI GTAC 4 cut(s) 1403, 1826, 1915, 2848
AfiI CCNNNNNNNGG 7 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374
AflII CTTAAG 2 cut(s) 1259, 2710
AflIII ACRYGT 2 cut(s) 572, 2379
AjnI CCWGG 6 cut(s) 30, 49, 1025, 1863, 1981, 2373
AleI CACNNNNGTG 1 cut(s) 563
Alw21I GWGCWC 4 cut(s) 322, 989, 2338, 2526
Alw26I GTCTC 2 cut(s) 1961, 2547
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 4 cut(s) 452, 1649, 1790, 2242
AlwNI CAGNNNCTG 1 cut(s) 3054
Ama87I CYCGRG 2 cut(s) 1271, 2202
Aor13HI TCCGGA 1 cut(s) 2864
AoxI GGCC 5 cut(s) 1180, 1809, 2398, 2748, 2875
ApaLI GTGCAC 1 cut(s) 318
ApoI RAATTY 4 cut(s) 385, 1904, 2052, 2207
AseI ATTAAT 2 cut(s) 102, 1434
Asp700I GAANNNNTTC 2 cut(s) 1158, 1385
Asp718I GGTACC 1 cut(s) 1913
AspA2I CCTAGG 1 cut(s) 2852
AspLEI GCGC 2 cut(s) 222, 2960
AspS9I GGNCC 4 cut(s) 1022, 1107, 2399, 2875
AvaI CYCGRG 2 cut(s) 1271, 2202
AvaII GGWCC 2 cut(s) 1022, 1107
AvrII CCTAGG 1 cut(s) 2852
AxyI CCTNAGG 1 cut(s) 850
BaeGI GKGCMC 2 cut(s) 322, 1062
BalI TGGCCA 1 cut(s) 2750
BanI GGYRCC 2 cut(s) 1119, 1913
BbsI GAAGAC 4 cut(s) 336, 1491, 2723, 2769
Bbv12I GWGCWC 4 cut(s) 322, 989, 2338, 2526
BceAI ACGGC 1 cut(s) 2283
BciT130I CCWGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
BciVI GTATCC 1 cut(s) 1936
BclI TGATCA 2 cut(s) 178, 379
BcoDI GTCTC 2 cut(s) 1961, 2547
BfaI CTAG 4 cut(s) 506, 897, 2622, 2853
BfmI CTRYAG 6 cut(s) 494, 561, 1092, 1265, 1648, 3019
BfrI CTTAAG 2 cut(s) 1259, 2710
BfuAI ACCTGC 2 cut(s) 487, 1305
BfuI GTATCC 1 cut(s) 1936
BglI GCCNNNNNGGC 1 cut(s) 1355
BlnI CCTAGG 1 cut(s) 2852
BlpI GCTNAGC 1 cut(s) 939
BmcAI AGTACT 1 cut(s) 2848
Bme1390I CCNGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
Bme18I GGWCC 2 cut(s) 1022, 1107
BmeT110I CYCGRG 2 cut(s) 1271, 2202
BmgT120I GGNCC 4 cut(s) 1022, 1107, 2399, 2875
BmrFI CCNGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
BmsI GCATC 8 cut(s) 52, 105, 394, 415, 473, 536, 1161, 2059
BoxI GACNNNNGTC 1 cut(s) 2790
BpiI GAAGAC 4 cut(s) 336, 1491, 2723, 2769
BpmI CTGGAG 6 cut(s) 263, 1775, 1985, 2004, 2244, 2707
Bpu1102I GCTNAGC 1 cut(s) 939
BsaBI GATNNNNATC 2 cut(s) 1659, 2479
BsaHI GRCGYC 1 cut(s) 7
BsaJI CCNNGG 6 cut(s) 50, 1025, 1350, 1765, 1864, 2852
BsaWI WCCGGW 1 cut(s) 2864
Bsc4I CCNNNNNNNGG 7 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374
Bse118I RCCGGY 3 cut(s) 1374, 1910, 2877
Bse1I ACTGG 6 cut(s) 202, 319, 334, 439, 892, 2751
Bse21I CCTNAGG 1 cut(s) 850
Bse3DI GCAATG 1 cut(s) 701
Bse8I GATNNNNATC 2 cut(s) 1659, 2479
BseAI TCCGGA 1 cut(s) 2864
BseBI CCWGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
BseDI CCNNGG 6 cut(s) 50, 1025, 1350, 1765, 1864, 2852
BseGI GGATG 8 cut(s) 298, 313, 876, 880, 1156, 1528, 2074, 2248
BseJI GATNNNNATC 2 cut(s) 1659, 2479
BseLI CCNNNNNNNGG 7 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374
BseMI GCAATG 1 cut(s) 701
BseMII CTCAG 5 cut(s) 792, 864, 930, 1587, 2037
BseNI ACTGG 6 cut(s) 202, 319, 334, 439, 892, 2751
BseRI GAGGAG 3 cut(s) 151, 2165, 2924
BseSI GKGCMC 2 cut(s) 322, 1062
BseYI CCCAGC 1 cut(s) 1832
BsgI GTGCAG 1 cut(s) 1932
Bsh1236I CGCG 1 cut(s) 1406
BshFI GGCC 5 cut(s) 1182, 1811, 2400, 2750, 2877
BshNI GGYRCC 2 cut(s) 1119, 1913
BsiHKAI GWGCWC 4 cut(s) 322, 989, 2338, 2526
BsiHKCI CYCGRG 2 cut(s) 1271, 2202
BsiSI CCGG 4 cut(s) 1375, 1911, 2865, 2878
BslFI GGGAC 2 cut(s) 1008, 2813
BslI CCNNNNNNNGG 7 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374
BsmAI GTCTC 2 cut(s) 1961, 2547
BsmBI CGTCTC 1 cut(s) 1961
BsmFI GGGAC 2 cut(s) 1008, 2813
BsmI GAATGC 4 cut(s) 253, 1475, 1887, 2018
BsnI GGCC 5 cut(s) 1182, 1811, 2400, 2750, 2877
BsoBI CYCGRG 2 cut(s) 1271, 2202
Bsp1286I GDGCHC 5 cut(s) 322, 989, 1062, 2338, 2526
Bsp13I TCCGGA 1 cut(s) 2864
Bsp1720I GCTNAGC 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 1350
BspACI CCGC 5 cut(s) 128, 596, 900, 1249, 2251
BspANI GGCC 5 cut(s) 1182, 1811, 2400, 2750, 2877
BspCNI CTCAG 5 cut(s) 793, 863, 931, 1586, 2036
BspEI TCCGGA 1 cut(s) 2864
BspFNI CGCG 1 cut(s) 1406
BspMAI CTGCAG 2 cut(s) 498, 3023
BspMI ACCTGC 2 cut(s) 487, 1305
BspPI GGATC 4 cut(s) 452, 1649, 1790, 2242
BspQI GCTCTTC 1 cut(s) 1440
BspT107I GGYRCC 2 cut(s) 1119, 1913
BspTI CTTAAG 2 cut(s) 1259, 2710
BsrDI GCAATG 1 cut(s) 701
BsrFI RCCGGY 3 cut(s) 1374, 1910, 2877
BsrI ACTGG 6 cut(s) 202, 319, 334, 439, 892, 2751
BssAI RCCGGY 3 cut(s) 1374, 1910, 2877
BssECI CCNNGG 6 cut(s) 50, 1025, 1350, 1765, 1864, 2852
BssNAI GTATAC 1 cut(s) 1617
BssNI GRCGYC 1 cut(s) 7
BssT1I CCWWGG 3 cut(s) 1350, 1765, 2852
Bst1107I GTATAC 1 cut(s) 1617
Bst2UI CCWGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
Bst4CI ACNGT 5 cut(s) 121, 1959, 2635, 2641, 2705
Bst6I CTCTTC 4 cut(s) 512, 1440, 2324, 2442
BstACI GRCGYC 1 cut(s) 7
BstAFI CTTAAG 2 cut(s) 1259, 2710
BstAPI GCANNNNNTGC 2 cut(s) 250, 2495
BstDEI CTNAG 6 cut(s) 801, 850, 939, 1411, 1573, 2023
BstDSI CCRYGG 1 cut(s) 1350
BstENI CCTNNNNNAGG 1 cut(s) 2372
BstF5I GGATG 8 cut(s) 298, 313, 876, 880, 1156, 1528, 2074, 2248
BstFNI CGCG 1 cut(s) 1406
BstHHI GCGC 2 cut(s) 222, 2960
BstMAI GTCTC 2 cut(s) 1961, 2547
BstNI CCWGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
BstNSI RCATGY 9 cut(s) 96, 253, 554, 576, 761, 1431, 1936, 2383, 2466
BstPAI GACNNNNGTC 1 cut(s) 2790
BstSCI CCNGG 6 cut(s) 30, 49, 1025, 1863, 1981, 2373
BstSFI CTRYAG 6 cut(s) 494, 561, 1092, 1265, 1648, 3019
BstSLI GKGCMC 2 cut(s) 322, 1062
BstUI CGCG 1 cut(s) 1406
BstV2I GAAGAC 4 cut(s) 336, 1491, 2723, 2769
BstX2I RGATCY 1 cut(s) 2247
BstXI CCANNNNNNTGG 4 cut(s) 38, 2301, 2422, 2992
BstYI RGATCY 1 cut(s) 2247
BstZ17I GTATAC 1 cut(s) 1617
Bsu36I CCTNAGG 1 cut(s) 850
BsuI GTATCC 1 cut(s) 1936
BsuRI GGCC 5 cut(s) 1182, 1811, 2400, 2750, 2877
BtgI CCRYGG 1 cut(s) 1350
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 8 cut(s) 298, 313, 876, 880, 1156, 1528, 2074, 2248
BtsI GCAGTG 3 cut(s) 215, 1827, 1949
BtsIMutI CAGTG 8 cut(s) 117, 215, 432, 1251, 1827, 1949, 2758, 3052
BveI ACCTGC 2 cut(s) 487, 1305
CaiI CAGNNNCTG 1 cut(s) 3054
CfoI GCGC 2 cut(s) 222, 2960
Cfr10I RCCGGY 3 cut(s) 1374, 1910, 2877
Cfr13I GGNCC 4 cut(s) 1022, 1107, 2399, 2875
CseI GACGC 1 cut(s) 168
Csp6I GTAC 4 cut(s) 1402, 1825, 1914, 2847
CspCI CAANNNNNGTGG 2 cut(s) 1588, 1623
CviQI GTAC 4 cut(s) 1402, 1825, 1914, 2847
DdeI CTNAG 6 cut(s) 801, 850, 939, 1411, 1573, 2023
DraI TTTAAA 1 cut(s) 2568
EaeI YGGCCR 1 cut(s) 2748
Eam1104I CTCTTC 4 cut(s) 512, 1440, 2324, 2442
EarI CTCTTC 4 cut(s) 512, 1440, 2324, 2442
Eco130I CCWWGG 3 cut(s) 1350, 1765, 2852
Eco47I GGWCC 2 cut(s) 1022, 1107
Eco57I CTGAAG 3 cut(s) 32, 2950, 2954
Eco81I CCTNAGG 1 cut(s) 850
Eco88I CYCGRG 2 cut(s) 1271, 2202
EcoNI CCTNNNNNAGG 1 cut(s) 2372
EcoO109I RGGNCCY 3 cut(s) 1022, 1107, 2399
EcoRI GAATTC 1 cut(s) 2207
EcoRII CCWGG 6 cut(s) 30, 49, 1025, 1863, 1981, 2373
EcoT14I CCWWGG 3 cut(s) 1350, 1765, 2852
EcoT22I ATGCAT 6 cut(s) 98, 255, 645, 1433, 1938, 2468
ErhI CCWWGG 3 cut(s) 1350, 1765, 2852
Esp3I CGTCTC 1 cut(s) 1961
FalI AAGNNNNNCTT 4 cut(s) 215, 247, 2927, 2959
FaqI GGGAC 2 cut(s) 1008, 2813
FauI CCCGC 2 cut(s) 589, 893
FauNDI CATATG 1 cut(s) 2662
FbaI TGATCA 2 cut(s) 178, 379
FblI GTMKAC 2 cut(s) 772, 1616
FokI GGATG 8 cut(s) 305, 320, 883, 887, 1163, 1535, 2081, 2255
FspBI CTAG 4 cut(s) 506, 897, 2622, 2853
FspI TGCGCA 1 cut(s) 221
GlaI GCGC 2 cut(s) 221, 2959
GsaI CCCAGC 1 cut(s) 1836
GsuI CTGGAG 6 cut(s) 263, 1775, 1985, 2004, 2244, 2707
HaeIII GGCC 5 cut(s) 1182, 1811, 2400, 2750, 2877
HapII CCGG 4 cut(s) 1375, 1911, 2865, 2878
HgaI GACGC 1 cut(s) 168
HhaI GCGC 2 cut(s) 222, 2960
Hin1I GRCGYC 1 cut(s) 7
Hin6I GCGC 2 cut(s) 220, 2958
HinP1I GCGC 2 cut(s) 220, 2958
HincII GTYRAC 2 cut(s) 1188, 2631
HindII GTYRAC 2 cut(s) 1188, 2631
HinfI GANTC 9 cut(s) 27, 863, 1603, 1660, 1846, 1988, 2768, 2774, 3028
HpaII CCGG 4 cut(s) 1375, 1911, 2865, 2878
Hpy99I CGWCG 1 cut(s) 2531
HpyAV CCTTC 7 cut(s) 205, 276, 898, 988, 1680, 1698, 2974
HpyCH4III ACNGT 5 cut(s) 121, 1959, 2635, 2641, 2705
HpyCH4IV ACGT 3 cut(s) 697, 1970, 2648
HpyF3I CTNAG 6 cut(s) 801, 850, 939, 1411, 1573, 2023
HpySE526I ACGT 3 cut(s) 697, 1970, 2648
Hsp92I GRCGYC 1 cut(s) 7
HspAI GCGC 2 cut(s) 220, 2958
KflI GGGWCCC 1 cut(s) 1022
Kpn2I TCCGGA 1 cut(s) 2864
KpnI GGTACC 1 cut(s) 1917
KroI GCCGGC 1 cut(s) 1374
KroNI GCCGGC 1 cut(s) 1376
Ksp22I TGATCA 2 cut(s) 178, 379
LguI GCTCTTC 1 cut(s) 1440
LmnI GCTCC 4 cut(s) 1597, 1718, 2341, 2911
LweI GCATC 8 cut(s) 52, 105, 394, 415, 473, 536, 1161, 2059
MaeI CTAG 4 cut(s) 506, 897, 2622, 2853
MaeII ACGT 3 cut(s) 697, 1970, 2648
MaeIII GTNAC 3 cut(s) 1640, 2635, 3035
MfeI CAATTG 3 cut(s) 36, 776, 2406
MflI RGATCY 1 cut(s) 2247
MhlI GDGCHC 5 cut(s) 322, 989, 1062, 2338, 2526
MlsI TGGCCA 1 cut(s) 2750
MluNI TGGCCA 1 cut(s) 2750
MlyI GAGTC 1 cut(s) 2783
MmeI TCCRAC 2 cut(s) 1818, 2282
Mox20I TGGCCA 1 cut(s) 2750
Mph1103I ATGCAT 6 cut(s) 98, 255, 645, 1433, 1938, 2468
MroI TCCGGA 1 cut(s) 2864
MroNI GCCGGC 1 cut(s) 1374
MroXI GAANNNNTTC 2 cut(s) 1158, 1385
MscI TGGCCA 1 cut(s) 2750
MslI CAYNNNNRTG 4 cut(s) 563, 2181, 2420, 2990
Msp20I TGGCCA 1 cut(s) 2750
MspA1I CMGCKG 2 cut(s) 184, 2762
MspCI CTTAAG 2 cut(s) 1259, 2710
MspI CCGG 4 cut(s) 1375, 1911, 2865, 2878
MspR9I CCNGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
MunI CAATTG 3 cut(s) 36, 776, 2406
Mva1269I GAATGC 4 cut(s) 253, 1475, 1887, 2018
MvaI CCWGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
MvnI CGCG 1 cut(s) 1406
NaeI GCCGGC 1 cut(s) 1376
NcoI CCATGG 1 cut(s) 1350
NdeI CATATG 1 cut(s) 2662
NgoMIV GCCGGC 1 cut(s) 1374
NmeAIII GCCGAG 1 cut(s) 2940
NmuCI GTSAC 2 cut(s) 1640, 2635
NsbI TGCGCA 1 cut(s) 221
NsiI ATGCAT 6 cut(s) 98, 255, 645, 1433, 1938, 2468
NspI RCATGY 9 cut(s) 96, 253, 554, 576, 761, 1431, 1936, 2383, 2466
OliI CACNNNNGTG 1 cut(s) 563
PaeI GCATGC 1 cut(s) 96
PaeR7I CTCGAG 2 cut(s) 1271, 2202
PciI ACATGT 2 cut(s) 572, 2379
PciSI GCTCTTC 1 cut(s) 1440
PctI GAATGC 4 cut(s) 253, 1475, 1887, 2018
PdiI GCCGGC 1 cut(s) 1376
PdmI GAANNNNTTC 2 cut(s) 1158, 1385
PfeI GAWTC 8 cut(s) 27, 863, 1603, 1660, 1846, 1988, 2768, 3028
PflMI CCANNNNNTGG 1 cut(s) 329
PleI GAGTC 1 cut(s) 2782
PpsI GAGTC 1 cut(s) 2782
PpuMI RGGWCCY 2 cut(s) 1022, 1107
PscI ACATGT 2 cut(s) 572, 2379
PshAI GACNNNNGTC 1 cut(s) 2790
PshBI ATTAAT 2 cut(s) 102, 1434
Psp5II RGGWCCY 2 cut(s) 1022, 1107
Psp6I CCWGG 6 cut(s) 30, 49, 1025, 1863, 1981, 2373
PspFI CCCAGC 1 cut(s) 1832
PspGI CCWGG 6 cut(s) 30, 49, 1025, 1863, 1981, 2373
PspPI GGNCC 4 cut(s) 1022, 1107, 2399, 2875
PspPPI RGGWCCY 2 cut(s) 1022, 1107
PspXI VCTCGAGB 1 cut(s) 1271
PsrI GAACNNNNNNTAC 2 cut(s) 1248, 1280
PstI CTGCAG 2 cut(s) 498, 3023
PstNI CAGNNNCTG 1 cut(s) 3054
PsuI RGATCY 1 cut(s) 2247
PvuII CAGCTG 2 cut(s) 184, 2762
RsaI GTAC 4 cut(s) 1403, 1826, 1915, 2848
RsaNI GTAC 4 cut(s) 1402, 1825, 1914, 2847
RseI CAYNNNNRTG 4 cut(s) 563, 2181, 2420, 2990
SapI GCTCTTC 1 cut(s) 1440
Sau96I GGNCC 4 cut(s) 1022, 1107, 2399, 2875
ScaI AGTACT 1 cut(s) 2848
SchI GAGTC 1 cut(s) 2783
ScrFI CCNGG 6 cut(s) 32, 51, 1027, 1865, 1983, 2375
SduI GDGCHC 5 cut(s) 322, 989, 1062, 2338, 2526
SfaNI GCATC 8 cut(s) 52, 105, 394, 415, 473, 536, 1161, 2059
SfcI CTRYAG 6 cut(s) 494, 561, 1092, 1265, 1648, 3019
Sfr274I CTCGAG 2 cut(s) 1271, 2202
SinI GGWCC 2 cut(s) 1022, 1107
SlaI CTCGAG 2 cut(s) 1271, 2202
SmiMI CAYNNNNRTG 4 cut(s) 563, 2181, 2420, 2990
SmlI CTYRAG 4 cut(s) 1259, 1271, 2202, 2710
SmoI CTYRAG 4 cut(s) 1259, 1271, 2202, 2710
SphI GCATGC 1 cut(s) 96
SsiI CCGC 5 cut(s) 128, 596, 900, 1249, 2251
SspI AATATT 1 cut(s) 972
SspMI CTAG 4 cut(s) 506, 897, 2622, 2853
StyD4I CCNGG 6 cut(s) 30, 49, 1025, 1863, 1981, 2373
StyI CCWWGG 3 cut(s) 1350, 1765, 2852
TaaI ACNGT 5 cut(s) 121, 1959, 2635, 2641, 2705
TaiI ACGT 3 cut(s) 700, 1973, 2651
TaqI TCGA 8 cut(s) 1272, 1975, 2203, 2324, 2430, 2526, 2700, 2921
TatI WGTACW 1 cut(s) 2846
TfiI GAWTC 8 cut(s) 27, 863, 1603, 1660, 1846, 1988, 2768, 3028
TscAI CASTG 7 cut(s) 124, 222, 439, 1258, 1834, 1956, 2758
TseFI GTSAC 2 cut(s) 1640, 2635
Tsp45I GTSAC 2 cut(s) 1640, 2635
TspGWI ACGGA 2 cut(s) 247, 569
TspRI CASTG 7 cut(s) 124, 222, 439, 1258, 1834, 1956, 2758
Van91I CCANNNNNTGG 1 cut(s) 329
Vha464I CTTAAG 2 cut(s) 1259, 2710
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 2 cut(s) 1022, 1107
VspI ATTAAT 2 cut(s) 102, 1434
XagI CCTNNNNNAGG 1 cut(s) 2372
XapI RAATTY 4 cut(s) 385, 1904, 2052, 2207
XceI RCATGY 9 cut(s) 96, 253, 554, 576, 761, 1431, 1936, 2383, 2466
XhoI CTCGAG 2 cut(s) 1271, 2202
XmaJI CCTAGG 1 cut(s) 2852
XmiI GTMKAC 2 cut(s) 772, 1616
XmnI GAANNNNTTC 2 cut(s) 1158, 1385
XspI CTAG 4 cut(s) 506, 897, 2622, 2853
ZrmI AGTACT 1 cut(s) 2848
Zsp2I ATGCAT 6 cut(s) 98, 255, 645, 1433, 1938, 2468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.