Rroxscaffold_4G00288810

ARF guanine-nucleotide exchange factor GNOM-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001807
Physical Location & Seq
Forward (+)
9483197 .. 9487759
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288810.1

Sequence Viewer

Length: 4188 bp
ATGGGGTGTCTAAAGGTGCAAAGTGGGATCAAGGCAGTTGAGGGAGAACCCAAGGACTCGGGTGGAATCTATTCGGACAAAGCTACTTTAGCATGCATCATTAACTCAGAAATAGGTACTGTATTAGCGGTTATGAGGAGGAATGTAAGATGGGGAGGGCGCTATGCATCGGATGATGATCAGATGGAACACTCTCTAATCCAGTCTTTGAAGGCTTTGCGCAAGAAAATATTTTCGTGGCAGCATCAGTTGAATAGCATCAGTCCAGCTGTGTATCTCAAGCCATTTCTGGACGTGATTAGATCTGACGAAACTGGTGCACCAATCACTGGTGTTGCTCTGGCATCTGTGTACAATATCTTAACACTAGACGTGATTGATCAGAACTCTGTCAACGTTGAAGATGCTATGCACTTGTTAGTTGATGCTATCACTGGCTGCCGATTTGAGGTGACTGACCCTGCATCAGAAGAAGTGGTACTTATGAAGATACTACAGGTTCTTTTGGCTTGCATGAAGAGTAAAGCATCTTTTATGTTGAGTAATCAGCAGGTTTGCACTATAGTGAATGCATGTTTCCGTATAGTTCATCAAGCAGGAACAAAAGGTGAGTTGTTGCAACGGATAGCTCGCCACACAATGCATGAGCTTGTTAGATGTATTTTCTCACACCTTCCAGATATCCGCAGCACCAAAAATGCATTGTCAAATGGAAGCAATTCCCTCAAACAAGAGACTTCAGGGCTCAATAACGAGCATGCACTTGAGAGTAGACAATTGGATAATGGCAGCATTAGTTCTGAGTATGACAGTCAACTATTATCCACAAATCCTGCTTTGCATGCTTCACCAGGCGTTGTAGAATCTGTGATAGGTGAGAAGACAACAGGGGCTTCTGACAGGAAAGATGCTGTGCAAAATGACTTGAATCTCATGACTGAGCCTTTTGGGGTTCCCAGCATGGTGGAAATTTTTCAGTTTCTTTGTTCTTTGTTAAACGTTGTTGAGCATATAGGATCGGGTCCTAAATCTAATCCCATAGCATTTGATGAAGATGTGCCTCTTTTTGCCTTGACCCTGGTTAATTCGGCTATAGAGTTGGGTGGGTCCTCCATTCAGCATCACCCCAAGTTATTGAGTTTGGTTCGGGATGAATTGTTTCAAAATCTAATGCAATTCGGCCTGTCAACGAGCCCACTTATTCTTTCAATGGTATGCAGCATTGTTCTTAATTTGTATCACCATCTGCGCACTGAACTTAAACTACAGCTTGAGGCTTTCTTTTCCTGCGTGATACTGAGGCTTGCACAAAGTAGGTATGGAGCTTCTTATCAACAGCAGGAGGTGGCCATGGAAGCTCTAGTTGATTTCTGCAGGCAGAAAAATTTCATGGTGGAGATGTACGCTAATCTAGATTGTGACATAACTTGCAGTAATATCTTTGAAGAGCTTGCTAATCTGTTGTCAAAGAGTGCGTTCCCAGTTAATAGCCCTTTGTCTTCCTTTCACATTCTTGCTTTGGATGGCCTTATTGCTGTTATTCAGGCAATGACAGAGAGGGTAGAGAATGGATCTTATAGTTTATCTCATACTCCAGTGACTCTTGAGGAGTATACTCCATTCTGGATGGTGAAGTGTGACAACTACAGTGATCCTAGTCATTGGGTTCCATTTGTTTGCCAGACGAAGTACATAAAGCGAAGGTTGATAACTGGTGCCGATCATTTTAACCATGACCCAAAGAAAGGGCTGGAATTCCTCCAAGGAATTCATCTCTTGCCTGAGAAACTTGATCCTCAAAGTGTGGCATGCTTTTTCAGGTATACGGCTGGGTTAGACAAGAATCTTATTGGGGATTTCTTGGGAAATCATGATGACTTTTGCATTCAGAAAATACAGAGGGTGCTTGAAGCATTCTCAGAGAGATACTATGAGCAATCCCCACTAATTCTAGCTGACAAGGATGCTGCTCTTCTTTTATCTTATTCAATCATAATGCTCAATACAGATCAGCACAATGTTCAGAATGAGATCCGCACTACTCCGGAACAAGGTGCTGGTTTTCCTGAAATGACTCCAAGCAGATGGATTGATTTGATGCACAAATCCAAGAAGAATGCTCCATTTATTGTATCTGATTCCAGAGCCTATCTTGATCAGGATATGTTCTCTATAGTGTCTGGGCCTACAATTGCTGCTATATCTGTGGTATTTGATAATGCTGAACAGGAAGAGGTTTACCAAACATGTCTCGATGGCTTTTTAGCTGTTGCCAAGATTTCAGCATGCTATCATCTTGAAGATGTTCTGGATGATCTGGTTGTGTCTCTCTGTAAGTTCACAACCCTCTTAAATTCATCATTTCTTGACGAACCTGAACTAGAATTTGGTGATGATATAAAAGCGAGAATGTCAACTGTGACAGTTTTCACAATTGCCAATCGGTATGGTGATTACATTCGTACAGGATGGAGAAATATTATTGACTGTATATTAAGATTACACAAGCTTGGTCTTCTTCCGGCTTGGGTGGCTCAAGATGCAGCTGATGAGTCGGAGTTTCCCTCTGAGACAGATCATGGAAAACCTATCACAAATTCCCAATCTAAAATTCATACGCCTTCCGTTAGTACTCCTAAGAAATCCTCTGGATTCATGGGTCGGTTTAGTCAGCTCTTATCTCTTGATACAGAGGAGCCGAAACCACAGCCTACTGAAGAAGAACTTGCAGCTCGTCAGCGCACTGTACAGACAGTTGAAGAGTGCCACATTGACAGCATATTTACCGAAAGCAAGTTTTTGCAGGCCGAATCCCTCTTACAGCTTTCACAAGCACTGATCTGGGCTGCAGGGCGACTCCAGAAAGGGAATAACTCTGCTGAGGATGAAGATACTGTTGTCTTTTGCTTGGAGTTGCTGATTGCTATTACCCTGAATAATAGGGATAGGATTATGCTTCTTTGGCAGGGTGTGTATGAGTTCATATCCAACATTGTGCAGTCAACTGTCATGCCTTGTGCCTTGGTAGAAAAGGCTGTCTTTGGACTTCTTCGGATTTGTCAGCGGCTGCTTCCCTATAAGGAAAACCTTGCTGATGATCTTTTGAGGTCACTGCAGCTTGTCTTGAAGCTTGATGCTCGGGTTGCCGATGCATACTGTGAGCAAATTACACAGGAAGTCAGTAGGCTTGTAAAGGCAAATGCCTCTCACATCAGATCTCAATTGGGGTGGCGCACAATTACGTCCCTACTTTCAATCACTGCTCGGCACCCGGATGCTTCTGAGGCTGGTTTTGACGCACTGTTCTTCATTATGTCAGGTGGAACTCACTTGTTGCCAGCAAATTATGTTCTCTGTGTCGATGCGTCACGGCAGTTTGCTGAGTCTCGTGTTGGGCAGGCGGATCGGTCTGTCTGCGCACTGGATCTTATGGCAGGTTCTGTTGATTGTTTAGCAAGATGGGCCCGTGAGGCTAAGCTATCTATGAATGAGGAGGATTCTGTGAAAATGTCTCAGGATATTGGGGAAATGTGGTTGAGGCTTGTTCAGGGATTGAGAAAAGTTTGTTTAGACCAAAGAGAAGTGGTTAGGAACCATGCTTTGTCGTTGTTACGGAACTGCTTGACAGGAGTTGATGGGATCCCTCTTCCATATGGTCTGTGGTTGCAGTGCTTTGATCTTGTGATTTTCACAGTGCTTGATGATTTGCTTGAAATTGCACAGGTTCACTCCCAAAAGGAGTACCGCAACATGGAAGGCACTCTTATCCTGGCCATGAAGCTCTTGTCCAAAGTGTTTTTGCAGCTACTTCCCGACCTATCACATTTAACAACCTTCTGTAAACTATGGCTGGGTGTTATTAGTCGAATGGAGAACTATATGAAGGTGAAAGTTGGAGGGAGGAAAAGTGAGAAGCTTCAGGACGAAGTACCTGAGCTACTTAAGAACACGTTGCTTGTAATGAATTTAAGGGGAGTGCTTGTTCAAAAGAGTGGTTCAGGTGAGGATGGCTTGTGGGAGCAGACATGGCTACATGTAAATAGAATTGCTCCATCTTTGCAAGCTGAGGTGCTTGCTCAAATTCTAGTGCAGTCACATACTGAGCAGGGTGAAACAGAGGGAGTATCTGCTGAACATGGTTCTTCAACCGAAACAATACCCTCTGGAGGTTCTGGCAGTGGTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Sec7 Sec7_N

Protein Analysis

1395

Amino Acids

155.31

Weight (kDa)

5.28

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 320 - 480 4e-40 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 566 - 629 7e-12 Sec7 domain
Sec7 PF01369 630 - 682 8.1e-15 Sec7 domain
TPR_28 PF23325 1178 - 1357 1e-11 GBF1-like, tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 221, 1250, 3418
Acc36I ACCTGC 2 cut(s) 541, 3425
AccB1I GGYRCC 2 cut(s) 1715, 3267
AccB7I CCANNNNNTGG 1 cut(s) 329
AccI GTMKAC 3 cut(s) 772, 1613, 1823
AccIII TCCGGA 1 cut(s) 2044
AciI CCGC 6 cut(s) 128, 685, 2035, 3064, 3401, 3745
AclI AACGTT 2 cut(s) 396, 999
AcoI YGGCCR 2 cut(s) 1347, 3771
AcuI CTGAAG 3 cut(s) 723, 2736, 3902
AfiI CCNNNNNNNGG 6 cut(s) 329, 448, 1745, 2051, 3751, 4166
AflII CTTAAG 1 cut(s) 3941
AflIII ACRYGT 3 cut(s) 2246, 3948, 4033
AjiI CACGTC 2 cut(s) 295, 373
AjnI CCWGG 3 cut(s) 850, 1077, 3768
AjuI GAANNNNNNNTTGG 4 cut(s) 2370, 2402, 2595, 2627
AleI CACNNNNGTG 1 cut(s) 563
AloI GAACNNNNNNTCC 2 cut(s) 3966, 3998
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 6 cut(s) 728, 2255, 2331, 2564, 3390, 3516
Alw44I GTGCAC 1 cut(s) 318
AlwNI CAGNNNCTG 2 cut(s) 3067, 3440
Ama87I CYCGRG 2 cut(s) 58, 3138
Aor13HI TCCGGA 1 cut(s) 2044
AoxI GGCC 7 cut(s) 1180, 1347, 1525, 2183, 2805, 3462, 3771
ApaI GGGCCC 1 cut(s) 3466
ApaLI GTGCAC 1 cut(s) 318
Asp700I GAANNNNTTC 6 cut(s) 70, 718, 972, 1158, 1385, 2304
AspLEI GCGC 6 cut(s) 162, 222, 1251, 2742, 3234, 3419
AspS9I GGNCC 5 cut(s) 1022, 1107, 2183, 3462, 3463
AsuC2I CCSGG 1 cut(s) 3272
AvaI CYCGRG 2 cut(s) 58, 3138
AvaII GGWCC 2 cut(s) 1022, 1107
BaeGI GKGCMC 2 cut(s) 322, 3466
BaeI ACNNNNGTAYC 2 cut(s) 2883, 2916
BalI TGGCCA 2 cut(s) 1349, 3773
BamHI GGATCC 1 cut(s) 3639
BanI GGYRCC 2 cut(s) 1715, 3267
BanII GRGCYC 3 cut(s) 747, 1196, 3466
BarI GAAGNNNNNNTAC 2 cut(s) 462, 494
BauI CACGAG 1 cut(s) 3387
BbsI GAAGAC 3 cut(s) 887, 1491, 2507
Bbv12I GWGCWC 1 cut(s) 322
BbvCI CCTCAGC 2 cut(s) 2880, 4065
BceAI ACGGC 2 cut(s) 1842, 3386
BcgI CGANNNNNNTGC 4 cut(s) 299, 333, 3386, 3420
BciT130I CCWGG 3 cut(s) 852, 1079, 3770
BclI TGATCA 3 cut(s) 178, 379, 2155
BcnI CCSGG 1 cut(s) 3272
BcoDI GTCTC 6 cut(s) 728, 2255, 2331, 2564, 3390, 3516
BfaI CTAG 7 cut(s) 368, 1361, 1412, 1656, 1952, 2381, 4085
BfmI CTRYAG 9 cut(s) 494, 561, 1092, 1265, 1372, 1645, 2172, 2847, 3113
BfoI RGCGCY 1 cut(s) 163
BfrI CTTAAG 1 cut(s) 3941
BfuAI ACCTGC 2 cut(s) 541, 3425
BglI GCCNNNNNGGC 1 cut(s) 3470
BglII AGATCT 2 cut(s) 302, 3215
BlpI GCTNAGC 1 cut(s) 3474
BmcAI AGTACT 1 cut(s) 2632
Bme1390I CCNGG 4 cut(s) 852, 1079, 3272, 3770
Bme18I GGWCC 2 cut(s) 1022, 1107
BmeT110I CYCGRG 2 cut(s) 58, 3138
BmgBI CACGTC 2 cut(s) 295, 373
BmgT120I GGNCC 5 cut(s) 1022, 1107, 2183, 3462, 3463
BmrFI CCNGG 4 cut(s) 852, 1079, 3272, 3770
BmrI ACTGGG 1 cut(s) 1475
BmuI ACTGGG 1 cut(s) 1475
BpiI GAAGAC 3 cut(s) 887, 1491, 2507
BplI GAGNNNNNCTC 2 cut(s) 2549, 2581
BpmI CTGGAG 3 cut(s) 1578, 2843, 4185
Bpu10I CCTNAGC 3 cut(s) 2880, 3933, 4065
Bpu1102I GCTNAGC 1 cut(s) 3474
BpuEI CTTGAG 5 cut(s) 263, 785, 1292, 1625, 2520
BpuMI CCSGG 1 cut(s) 3272
BsaBI GATNNNNATC 1 cut(s) 177
BsaJI CCNNGG 5 cut(s) 51, 1077, 1350, 1762, 3021
BsaWI WCCGGW 1 cut(s) 2044
BsaXI ACNNNNNCTCC 4 cut(s) 3892, 3922, 3966, 3996
Bsc4I CCNNNNNNNGG 6 cut(s) 329, 448, 1745, 2051, 3751, 4166
Bse1I ACTGG 8 cut(s) 202, 319, 334, 439, 1481, 1595, 1717, 3426
Bse3DI GCAATG 1 cut(s) 1554
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 2044
BseBI CCWGG 3 cut(s) 852, 1079, 3770
BseDI CCNNGG 5 cut(s) 51, 1077, 1350, 1762, 3021
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 6 cut(s) 329, 448, 1745, 2051, 3751, 4166
BseMI GCAATG 1 cut(s) 1554
BseNI ACTGG 8 cut(s) 202, 319, 334, 439, 1481, 1595, 1717, 3426
BseRI GAGGAG 4 cut(s) 151, 1622, 2708, 3506
BseSI GKGCMC 2 cut(s) 322, 3466
BseYI CCCAGC 3 cut(s) 956, 1829, 3850
BsgI GTGCAG 2 cut(s) 3017, 4109
BshFI GGCC 7 cut(s) 1182, 1349, 1527, 2185, 2807, 3464, 3773
BshNI GGYRCC 2 cut(s) 1715, 3267
BsiHKAI GWGCWC 1 cut(s) 322
BsiHKCI CYCGRG 2 cut(s) 58, 3138
BsiSI CCGG 3 cut(s) 2045, 2522, 3272
BslFI GGGAC 1 cut(s) 3229
BslI CCNNNNNNNGG 6 cut(s) 329, 448, 1745, 2051, 3751, 4166
BsmAI GTCTC 6 cut(s) 728, 2255, 2331, 2564, 3390, 3516
BsmFI GGGAC 1 cut(s) 3229
BsmI GAATGC 4 cut(s) 574, 1884, 1913, 2122
BsnI GGCC 7 cut(s) 1182, 1349, 1527, 2185, 2807, 3464, 3773
BsoBI CYCGRG 2 cut(s) 58, 3138
Bsp120I GGGCCC 1 cut(s) 3462
Bsp1286I GDGCHC 4 cut(s) 322, 747, 1196, 3466
Bsp13I TCCGGA 1 cut(s) 2044
Bsp1407I TGTACA 2 cut(s) 351, 2746
Bsp1720I GCTNAGC 1 cut(s) 3474
Bsp19I CCATGG 1 cut(s) 1350
BspACI CCGC 6 cut(s) 128, 685, 2035, 3064, 3401, 3745
BspANI GGCC 7 cut(s) 1182, 1349, 1527, 2185, 2807, 3464, 3773
BspEI TCCGGA 1 cut(s) 2044
BspHI TCATGA 2 cut(s) 933, 1870
BspMAI CTGCAG 3 cut(s) 1376, 2851, 3117
BspMI ACCTGC 2 cut(s) 541, 3425
BspQI GCTCTTC 2 cut(s) 1440, 1977
BspT107I GGYRCC 2 cut(s) 1715, 3267
BspTI CTTAAG 1 cut(s) 3941
BsrDI GCAATG 1 cut(s) 1554
BsrGI TGTACA 2 cut(s) 351, 2746
BsrI ACTGG 8 cut(s) 202, 319, 334, 439, 1481, 1595, 1717, 3426
BssECI CCNNGG 5 cut(s) 51, 1077, 1350, 1762, 3021
BssNAI GTATAC 2 cut(s) 1614, 1824
BssSI CACGAG 1 cut(s) 3387
BssT1I CCWWGG 4 cut(s) 51, 1350, 1762, 3021
Bst1107I GTATAC 2 cut(s) 1614, 1824
Bst2BI CACGAG 1 cut(s) 3387
Bst2UI CCWGG 3 cut(s) 852, 1079, 3770
Bst6I CTCTTC 6 cut(s) 512, 1440, 1977, 2226, 2754, 3651
BstAFI CTTAAG 1 cut(s) 3941
BstAUI TGTACA 2 cut(s) 351, 2746
BstDSI CCRYGG 1 cut(s) 1350
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 6 cut(s) 162, 222, 1251, 2742, 3234, 3419
BstMAI GTCTC 6 cut(s) 728, 2255, 2331, 2564, 3390, 3516
BstNI CCWGG 3 cut(s) 852, 1079, 3770
BstNSI RCATGY 8 cut(s) 96, 576, 761, 845, 1812, 2250, 2289, 4037
BstSCI CCNGG 4 cut(s) 850, 1077, 3270, 3768
BstSFI CTRYAG 9 cut(s) 494, 561, 1092, 1265, 1372, 1645, 2172, 2847, 3113
BstSLI GKGCMC 2 cut(s) 322, 3466
BstV2I GAAGAC 3 cut(s) 887, 1491, 2507
BstX2I RGATCY 6 cut(s) 302, 1571, 2031, 3215, 3424, 3639
BstXI CCANNNNNNTGG 2 cut(s) 964, 2085
BstYI RGATCY 6 cut(s) 302, 1571, 2031, 3215, 3424, 3639
BstZ17I GTATAC 2 cut(s) 1614, 1824
BsuRI GGCC 7 cut(s) 1182, 1349, 1527, 2185, 2807, 3464, 3773
BtgI CCRYGG 1 cut(s) 1350
BtrI CACGTC 2 cut(s) 295, 373
BtsI GCAGTG 4 cut(s) 3110, 3258, 3674, 4183
BveI ACCTGC 2 cut(s) 541, 3425
CaiI CAGNNNCTG 2 cut(s) 3067, 3440
CciI TCATGA 2 cut(s) 933, 1870
CfoI GCGC 6 cut(s) 162, 222, 1251, 2742, 3234, 3419
Cfr13I GGNCC 5 cut(s) 1022, 1107, 2183, 3462, 3463
CseI GACGC 2 cut(s) 3305, 3354
CspCI CAANNNNNGTGG 2 cut(s) 3209, 3244
EaeI YGGCCR 2 cut(s) 1347, 3771
Eam1104I CTCTTC 6 cut(s) 512, 1440, 1977, 2226, 2754, 3651
EarI CTCTTC 6 cut(s) 512, 1440, 1977, 2226, 2754, 3651
EciI GGCGGA 1 cut(s) 3416
Eco130I CCWWGG 4 cut(s) 51, 1350, 1762, 3021
Eco24I GRGCYC 3 cut(s) 747, 1196, 3466
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 2 cut(s) 1022, 1107
Eco57I CTGAAG 3 cut(s) 723, 2736, 3902
Eco88I CYCGRG 2 cut(s) 58, 3138
EcoO109I RGGNCCY 2 cut(s) 1022, 1107
EcoRI GAATTC 2 cut(s) 1754, 1767
EcoRII CCWGG 3 cut(s) 850, 1077, 3768
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 4 cut(s) 51, 1350, 1762, 3021
EcoT22I ATGCAT 6 cut(s) 98, 169, 574, 645, 703, 3154
EcoT38I GRGCYC 3 cut(s) 747, 1196, 3466
ErhI CCWWGG 4 cut(s) 51, 1350, 1762, 3021
FalI AAGNNNNNCTT 8 cut(s) 465, 497, 2709, 2741, 3023, 3055, 3747, 3779
FaqI GGGAC 1 cut(s) 3229
FauNDI CATATG 1 cut(s) 3652
FbaI TGATCA 3 cut(s) 178, 379, 2155
FblI GTMKAC 3 cut(s) 772, 1613, 1823
FriOI GRGCYC 3 cut(s) 747, 1196, 3466
FspBI CTAG 7 cut(s) 368, 1361, 1412, 1656, 1952, 2381, 4085
FspI TGCGCA 3 cut(s) 221, 1250, 3418
GlaI GCGC 6 cut(s) 161, 221, 1250, 2741, 3233, 3418
GsaI CCCAGC 3 cut(s) 960, 1833, 3854
GsuI CTGGAG 3 cut(s) 1578, 2843, 4185
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 7 cut(s) 1182, 1349, 1527, 2185, 2807, 3464, 3773
HapII CCGG 3 cut(s) 2045, 2522, 3272
HgaI GACGC 2 cut(s) 3305, 3354
HhaI GCGC 6 cut(s) 162, 222, 1251, 2742, 3234, 3419
Hin6I GCGC 6 cut(s) 160, 220, 1249, 2740, 3232, 3417
HinP1I GCGC 6 cut(s) 160, 220, 1249, 2740, 3232, 3417
HincII GTYRAC 5 cut(s) 394, 815, 1188, 2415, 3003
HindII GTYRAC 5 cut(s) 394, 815, 1188, 2415, 3003
HindIII AAGCTT 3 cut(s) 2507, 3128, 3914
HpaII CCGG 3 cut(s) 2045, 2522, 3272
HpyAV CCTTC 7 cut(s) 205, 683, 1695, 2631, 3749, 3844, 3877
HpyCH4IV ACGT 6 cut(s) 294, 372, 396, 999, 3242, 3950
HpySE526I ACGT 6 cut(s) 294, 372, 396, 999, 3242, 3950
HspAI GCGC 6 cut(s) 160, 220, 1249, 2740, 3232, 3417
Kpn2I TCCGGA 1 cut(s) 2044
Ksp22I TGATCA 3 cut(s) 178, 379, 2155
LguI GCTCTTC 2 cut(s) 1440, 1977
LmnI GCTCC 5 cut(s) 1322, 2125, 2695, 4018, 4054
MaeI CTAG 7 cut(s) 368, 1361, 1412, 1656, 1952, 2381, 4085
MaeII ACGT 6 cut(s) 294, 372, 396, 999, 3242, 3950
MaeIII GTNAC 9 cut(s) 451, 1418, 1597, 1637, 2419, 3108, 3366, 3609, 4092
MfeI CAATTG 4 cut(s) 776, 2190, 2433, 3221
MflI RGATCY 6 cut(s) 302, 1571, 2031, 3215, 3424, 3639
MhlI GDGCHC 4 cut(s) 322, 747, 1196, 3466
MlsI TGGCCA 2 cut(s) 1349, 3773
MluNI TGGCCA 2 cut(s) 1349, 3773
MlyI GAGTC 6 cut(s) 50, 1594, 2068, 2561, 2850, 3392
MmeI TCCRAC 3 cut(s) 2535, 3012, 3874
Mox20I TGGCCA 2 cut(s) 1349, 3773
Mph1103I ATGCAT 6 cut(s) 98, 169, 574, 645, 703, 3154
MroI TCCGGA 1 cut(s) 2044
MroXI GAANNNNTTC 6 cut(s) 70, 718, 972, 1158, 1385, 2304
MscI TGGCCA 2 cut(s) 1349, 3773
MslI CAYNNNNRTG 2 cut(s) 563, 3273
Msp20I TGGCCA 2 cut(s) 1349, 3773
MspA1I CMGCKG 3 cut(s) 269, 2546, 3064
MspCI CTTAAG 1 cut(s) 3941
MspI CCGG 3 cut(s) 2045, 2522, 3272
MspR9I CCNGG 4 cut(s) 852, 1079, 3272, 3770
MunI CAATTG 4 cut(s) 776, 2190, 2433, 3221
Mva1269I GAATGC 4 cut(s) 574, 1884, 1913, 2122
MvaI CCWGG 3 cut(s) 852, 1079, 3770
NciI CCSGG 1 cut(s) 3272
NcoI CCATGG 1 cut(s) 1350
NdeI CATATG 1 cut(s) 3652
NmeAIII GCCGAG 1 cut(s) 3244
NmuCI GTSAC 8 cut(s) 451, 1418, 1597, 1637, 2419, 3108, 3366, 4092
NsbI TGCGCA 3 cut(s) 221, 1250, 3418
NsiI ATGCAT 6 cut(s) 98, 169, 574, 645, 703, 3154
NspI RCATGY 8 cut(s) 96, 576, 761, 845, 1812, 2250, 2289, 4037
OliI CACNNNNGTG 1 cut(s) 563
PaeI GCATGC 5 cut(s) 96, 761, 845, 1812, 2289
PagI TCATGA 2 cut(s) 933, 1870
PciI ACATGT 2 cut(s) 2246, 4033
PciSI GCTCTTC 2 cut(s) 1440, 1977
PctI GAATGC 4 cut(s) 574, 1884, 1913, 2122
PdmI GAANNNNTTC 6 cut(s) 70, 718, 972, 1158, 1385, 2304
PfeI GAWTC 8 cut(s) 66, 863, 928, 1843, 2138, 2652, 2810, 3497
PflMI CCANNNNNTGG 1 cut(s) 329
PleI GAGTC 6 cut(s) 50, 1594, 2068, 2560, 2850, 3391
PpsI GAGTC 6 cut(s) 50, 1594, 2068, 2560, 2850, 3391
PpuMI RGGWCCY 2 cut(s) 1022, 1107
PscI ACATGT 2 cut(s) 2246, 4033
Psp1406I AACGTT 2 cut(s) 396, 999
Psp5II RGGWCCY 2 cut(s) 1022, 1107
Psp6I CCWGG 3 cut(s) 850, 1077, 3768
PspFI CCCAGC 3 cut(s) 956, 1829, 3850
PspGI CCWGG 3 cut(s) 850, 1077, 3768
PspOMI GGGCCC 1 cut(s) 3462
PspPI GGNCC 5 cut(s) 1022, 1107, 2183, 3462, 3463
PspPPI RGGWCCY 2 cut(s) 1022, 1107
PsrI GAACNNNNNNTAC 2 cut(s) 1248, 1280
PstI CTGCAG 3 cut(s) 1376, 2851, 3117
PstNI CAGNNNCTG 2 cut(s) 3067, 3440
PsuI RGATCY 6 cut(s) 302, 1571, 2031, 3215, 3424, 3639
PvuII CAGCTG 2 cut(s) 269, 2546
RseI CAYNNNNRTG 2 cut(s) 563, 3273
SapI GCTCTTC 2 cut(s) 1440, 1977
Sau96I GGNCC 5 cut(s) 1022, 1107, 2183, 3462, 3463
ScaI AGTACT 1 cut(s) 2632
SchI GAGTC 6 cut(s) 50, 1594, 2068, 2561, 2850, 3392
ScrFI CCNGG 4 cut(s) 852, 1079, 3272, 3770
SduI GDGCHC 4 cut(s) 322, 747, 1196, 3466
SfcI CTRYAG 9 cut(s) 494, 561, 1092, 1265, 1372, 1645, 2172, 2847, 3113
SinI GGWCC 2 cut(s) 1022, 1107
SmiMI CAYNNNNRTG 2 cut(s) 563, 3273
SmlI CTYRAG 6 cut(s) 278, 764, 1271, 1604, 2535, 3941
SmoI CTYRAG 6 cut(s) 278, 764, 1271, 1604, 2535, 3941
SphI GCATGC 5 cut(s) 96, 761, 845, 1812, 2289
SsiI CCGC 6 cut(s) 128, 685, 2035, 3064, 3401, 3745
SspI AATATT 2 cut(s) 231, 2479
SspMI CTAG 7 cut(s) 368, 1361, 1412, 1656, 1952, 2381, 4085
StyD4I CCNGG 4 cut(s) 850, 1077, 3270, 3768
StyI CCWWGG 4 cut(s) 51, 1350, 1762, 3021
TaiI ACGT 6 cut(s) 297, 375, 399, 1002, 3245, 3953
TaqI TCGA 3 cut(s) 2253, 3360, 3865
TaqII GACCGA 1 cut(s) 3396
TatI WGTACW 4 cut(s) 351, 1689, 2630, 2746
TauI GCSGC 1 cut(s) 3067
TfiI GAWTC 8 cut(s) 66, 863, 928, 1843, 2138, 2652, 2810, 3497
TseFI GTSAC 8 cut(s) 451, 1418, 1597, 1637, 2419, 3108, 3366, 4092
Tsp45I GTSAC 8 cut(s) 451, 1418, 1597, 1637, 2419, 3108, 3366, 4092
TspGWI ACGGA 4 cut(s) 569, 637, 2614, 3628
Van91I CCANNNNNTGG 1 cut(s) 329
Vha464I CTTAAG 1 cut(s) 3941
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 2 cut(s) 1022, 1107
XbaI TCTAGA 1 cut(s) 1411
XceI RCATGY 8 cut(s) 96, 576, 761, 845, 1812, 2250, 2289, 4037
XcmI CCANNNNNNNNNTGG 1 cut(s) 3657
XmiI GTMKAC 3 cut(s) 772, 1613, 1823
XmnI GAANNNNTTC 6 cut(s) 70, 718, 972, 1158, 1385, 2304
XspI CTAG 7 cut(s) 368, 1361, 1412, 1656, 1952, 2381, 4085
ZrmI AGTACT 1 cut(s) 2632
Zsp2I ATGCAT 6 cut(s) 98, 169, 574, 645, 703, 3154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.