pycom01g15940

ARF guanine-nucleotide exchange factor GNOM-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001807
Physical Location & Seq
Reverse (-)
15667255 .. 15669865
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15940.1

Sequence Viewer

Length: 2466 bp
ATGGGGCGTCTAAAGCTGCAAACTGGGATCAAGGCAATTGAGGAAGAACCCGAGGACTGTGAGGCAACCAATTCAAATAAAGCTACATTGGCATGCATCATCAATTCAGAAATAGGTTCTGTATTAGCGGTTATGAGGAGGAATGTAAGATGGGGCAGTCGTTATATATCCGGTGATGATCAGCTGGAACACTCTTTAATCCAGTCTTTAAAGGCATTGCGCAAGCAAATTTTTTCATGGCAGCATCAGTGGCATACCATTAATCCTGTTGTGTATCTCCAGCCATTTTTGGATGTGATTAGATCTGATGAAACTGGTGCACCAATCACTGGTGTTGCTTTGTCATCTGTTTATAATATCTTAACACTTGATGTGATTGATCAAAATTCCGTAAATGTTGAAGATGCCATGAACTTGTTAGTTGATGCCATCACTAGTTGTCGATTTGAGGGCATCGATCCTGCATCAGAGGAAGTGGTACTGATGAAGATACTACAGGTTCTTCTGGCTTGCATGAAGAGTAAAGCATCTGTTATGTTGAGTAACCAGCATGTTTGCACTATAGTGAATACATGTTTTCGTATAGTTCATCAAGCAGGAACAAAATGCGAGTTGTTGCAACGGATAGCTCGCCACACAATGCATGAACTTGTTAGATGTATTTTCTCACACCTTCCTGATGTTCACGACACTGAACGTGCACTGTTAAATGGAAGTACTACAAGAAAACAAGAGATTGCCGGGCTAAATAATGAGTATGCTTTTGGGAGTAGACAATTGGAGAATGGCAATGTGAGTTCTGGGTATGATAGTCAACCATTATCCACAAGTCCTGCCTCAAATGCTTCTTCAGGCCTGGTAGCACCTCGGATTGATGAAAGTGTACTCACGGTTTCTACTGAGAAGGAGGCTGTTCAATATGATTTGCATCTCATGACTGAGCCATATGGGGTACCCTGCATGGTGGAAATATTTCACTTTCTGTGCTCTTTGTTAAATGTTTCTGAGCATATGGGAATGGGACCCAGATCTAATACCATAACATTTGATGAAGATGTGCCTCTTTTTGCCTTGGTTATGATTAATTCAGCTATAGAGTTGGGTGGATCCCACATTCAGAATCATCCCAAATTATTGAGTTTAGTTCAGGATGAATTGTTTCGAAATCTTATGCAGTTTGGTTTGTCAACGAGTCCACTTATTCTTTCAATGGTATGCAGCATTGTTCTTAATTTATACCACCACTTGCGCACTGAACTTAAACTACAGCTTGAGGCCTTCTTTTCTTGTGTGATTTTGAGGCTTGCACAAAGTAAATATGGAGCTTCTTATCAGCAGCAGGAGGTTGCTATGGAGGTTCTTGTTGACTTCTGCAGGCAGAAAAATTTCATGGTGGAGATGTACGCTAACTTAGATTGTGACATAACATGCAGCAATGTCTTTGAAGAACTTGCTAATCTGTTGTCAAAGAGTGCATTCCCTGTCAATTGCCCTTTGTCTTCAATTCACATTCTTGCTTTGGATGGCCTTATTGCTGTTATTCAAGGAATGGCCGAAAGGGTGGGGAATGGATCAGTTTCTGCACATACGCCAGTGAATCTTGAAGAGTATACTCCATTCTGGATGGTGAAGTGTGACAACTACAGTGATCCTAGTAATTGGGTTCCATTTGTTCGCCGGAGGAAGTACATAAAGAGAAGGTTAATGATTGGAGCTGATCATTTTAACCGTGACCCAAAGAAAGGACTGGAGTTCCTCCAAGGAACATATCTGTTGCATGACAAACTCGATCCACAAAGCGTGGCCTGCTTTTTCAGGTACACTGCTGGGTTGGACAAGAATCTAGTTGGGGATTTCTTGGGAAATCATGATGAGTTTTGTGTTCAGGTTCTTCATAAATTTGCCGGTACCTTTGATTTCGCAGATATGAACTTGGATACTGCACTCCGACTGTTTTTGGAGACGTTTCGATTGCCTGGAGAATCACAAAAGATACAAAGGGTGCTTGAAGCATTCTCAGAGAGATATTATGAGCAATCACCACTAATTCTAGCTAACAAGGATGCTGCGCTTTTGCTGTCATATTCACTCATAATGCTCAATACAGATCAACACAATGTTCAGGTAAAGAAGAAAATGACGGAGGAGGATTTCATTCGGAATAATCGGCACATCAATGGAGGCAGTGATCTTCCTCGAGAATTTCTGACAGAGCTTTACCACTCAATATGCAAGAACGAGATCCGAACAACTCCAGAACAAGGTGCTGGTTATCCTGAAATGACCCCAAGCCGGTGGATTGATTTAATGCACAAATCTAAGAAGAAAGCTCCATTTATCATGTCTGATTCCAGAGCCTACCTAGACCATGATATGTTTGCTATAATGTCGGGCCCTACAATTGCTGCTATCTCTGTGGTATTTGATCTCACGCAGAACATGAAGAGATTTACCAAACTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Sec7 Sec7_N

Protein Analysis

822

Amino Acids

92.58

Weight (kDa)

5.85

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 318 - 480 1.1e-40 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 565 - 748 3.4e-71 Sec7 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
Acc16I TGCGCA 2 cut(s) 221, 1250
Acc65I GGTACC 2 cut(s) 952, 1907
AccB1I GGYRCC 2 cut(s) 952, 1907
AccB7I CCANNNNNTGG 1 cut(s) 329
AccI GTMKAC 2 cut(s) 772, 1610
AciI CCGC 1 cut(s) 128
AclWI GGATC 8 cut(s) 35, 452, 1101, 1114, 1579, 1643, 1784, 2236
AcoI YGGCCR 1 cut(s) 1551
AcsI RAATTY 5 cut(s) 228, 385, 1384, 1898, 2201
AcuI CTGAAG 1 cut(s) 834
AcyI GRCGYC 1 cut(s) 7
AfaI GTAC 8 cut(s) 480, 718, 885, 954, 1403, 1688, 1820, 1909
AfiI CCNNNNNNNGG 4 cut(s) 329, 1742, 2261, 2292
AflIII ACRYGT 1 cut(s) 572
AgsI TTSAA 9 cut(s) 75, 401, 917, 1209, 1445, 1503, 1544, 1604, 2009
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 2 cut(s) 855, 1975
AleI CACNNNNGTG 1 cut(s) 563
Alw21I GWGCWC 3 cut(s) 322, 703, 989
Alw26I GTCTC 1 cut(s) 1955
Alw44I GTGCAC 2 cut(s) 318, 699
AlwI GGATC 8 cut(s) 35, 452, 1101, 1114, 1579, 1643, 1784, 2236
AlwNI CAGNNNCTG 1 cut(s) 1580
Ama87I CYCGRG 2 cut(s) 50, 2196
AoxI GGCC 6 cut(s) 853, 1275, 1525, 1551, 1803, 2392
ApaI GGGCCC 1 cut(s) 2396
ApaLI GTGCAC 2 cut(s) 318, 699
ApeKI GCWGC 7 cut(s) 16, 241, 1218, 1336, 1431, 2066, 2405
ApoI RAATTY 5 cut(s) 228, 385, 1384, 1898, 2201
AseI ATTAAT 2 cut(s) 261, 1083
Asp700I GAANNNNTTC 3 cut(s) 972, 1158, 1385
Asp718I GGTACC 2 cut(s) 952, 1907
AspLEI GCGC 3 cut(s) 222, 1251, 2071
AspS9I GGNCC 3 cut(s) 1022, 2392, 2393
AsuC2I CCSGG 1 cut(s) 742
AsuHPI GGTGA 3 cut(s) 185, 1639, 2031
AsuII TTCGAA 1 cut(s) 1162
AvaI CYCGRG 2 cut(s) 50, 2196
AvaII GGWCC 1 cut(s) 1022
BaeGI GKGCMC 3 cut(s) 322, 703, 2396
BamHI GGATCC 1 cut(s) 1106
BanI GGYRCC 2 cut(s) 952, 1907
BanII GRGCYC 1 cut(s) 2396
BbsI GAAGAC 1 cut(s) 1491
Bbv12I GWGCWC 3 cut(s) 322, 703, 989
BbvI GCAGC 7 cut(s) 3, 253, 1230, 1348, 1443, 2053, 2392
BccI CCATC 4 cut(s) 144, 437, 1517, 1618
BciT130I CCWGG 2 cut(s) 857, 1977
BciVI GTATCC 1 cut(s) 1930
BclI TGATCA 3 cut(s) 178, 379, 1717
BcnI CCSGG 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 1955
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 5 cut(s) 435, 1653, 1844, 2051, 2363
BfmI CTRYAG 6 cut(s) 494, 561, 1092, 1265, 1372, 1642
BfuI GTATCC 1 cut(s) 1930
BglII AGATCT 2 cut(s) 302, 1028
BisI GCNGC 7 cut(s) 17, 242, 1219, 1337, 1432, 2067, 2406
BlsI GCNGC 7 cut(s) 18, 243, 1220, 1338, 1433, 2068, 2407
BmcAI AGTACT 1 cut(s) 718
Bme1390I CCNGG 3 cut(s) 742, 857, 1977
Bme18I GGWCC 1 cut(s) 1022
BmeT110I CYCGRG 2 cut(s) 50, 2196
BmgT120I GGNCC 3 cut(s) 1022, 2392, 2393
BmiI GGNNCC 7 cut(s) 954, 1023, 1024, 1108, 1665, 1909, 2394
BmrFI CCNGG 3 cut(s) 742, 857, 1977
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 9 cut(s) 105, 253, 394, 415, 462, 473, 536, 937, 2053
BmuI ACTGGG 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 1491
BpmI CTGGAG 4 cut(s) 263, 1769, 1998, 2238
Bpu14I TTCGAA 1 cut(s) 1162
BpuEI CTTGAG 1 cut(s) 1292
BpuMI CCSGG 1 cut(s) 742
Bsa29I ATCGAT 1 cut(s) 456
BsaBI GATNNNNATC 1 cut(s) 927
BsaHI GRCGYC 1 cut(s) 7
BsaJI CCNNGG 4 cut(s) 51, 866, 1071, 1759
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 1742, 2261, 2292
Bse118I RCCGGY 2 cut(s) 1904, 2292
Bse1I ACTGG 6 cut(s) 28, 202, 319, 334, 1592, 1752
Bse3DI GCAATG 3 cut(s) 215, 796, 1441
Bse8I GATNNNNATC 1 cut(s) 927
BseBI CCWGG 2 cut(s) 857, 1977
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 4 cut(s) 51, 866, 1071, 1759
BseGI GGATG 6 cut(s) 298, 1123, 1156, 1528, 1629, 2068
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 4 cut(s) 329, 1742, 2261, 2292
BseMI GCAATG 3 cut(s) 215, 796, 1441
BseMII CTCAG 4 cut(s) 891, 930, 996, 2031
BseNI ACTGG 6 cut(s) 28, 202, 319, 334, 1592, 1752
BseRI GAGGAG 2 cut(s) 151, 2159
BseSI GKGCMC 3 cut(s) 322, 703, 2396
BseXI GCAGC 7 cut(s) 3, 253, 1230, 1348, 1443, 2053, 2392
BseYI CCCAGC 1 cut(s) 1826
BsgI GTGCAG 2 cut(s) 1566, 1926
BshFI GGCC 6 cut(s) 855, 1277, 1527, 1553, 1805, 2394
BshNI GGYRCC 2 cut(s) 952, 1907
BshVI ATCGAT 1 cut(s) 456
BsiHKAI GWGCWC 3 cut(s) 322, 703, 989
BsiHKCI CYCGRG 2 cut(s) 50, 2196
BsiSI CCGG 5 cut(s) 171, 741, 1678, 1905, 2293
BslFI GGGAC 1 cut(s) 1035
BslI CCNNNNNNNGG 4 cut(s) 329, 1742, 2261, 2292
BsmAI GTCTC 1 cut(s) 1955
BsmBI CGTCTC 1 cut(s) 1955
BsmFI GGGAC 1 cut(s) 1035
BsmI GAATGC 2 cut(s) 1475, 2012
BsnI GGCC 6 cut(s) 855, 1277, 1527, 1553, 1805, 2394
BsoBI CYCGRG 2 cut(s) 50, 2196
Bsp119I TTCGAA 1 cut(s) 1162
Bsp120I GGGCCC 1 cut(s) 2392
Bsp1286I GDGCHC 4 cut(s) 322, 703, 989, 2396
BspACI CCGC 1 cut(s) 128
BspANI GGCC 6 cut(s) 855, 1277, 1527, 1553, 1805, 2394
BspCNI CTCAG 4 cut(s) 892, 931, 997, 2030
BspDI ATCGAT 1 cut(s) 456
BspHI TCATGA 2 cut(s) 933, 1867
BspLI GGNNCC 7 cut(s) 954, 1023, 1024, 1108, 1665, 1909, 2394
BspMAI CTGCAG 1 cut(s) 1376
BspPI GGATC 8 cut(s) 35, 452, 1101, 1114, 1579, 1643, 1784, 2236
BspT104I TTCGAA 1 cut(s) 1162
BspT107I GGYRCC 2 cut(s) 952, 1907
BsrDI GCAATG 3 cut(s) 215, 796, 1441
BsrFI RCCGGY 2 cut(s) 1904, 2292
BsrI ACTGG 6 cut(s) 28, 202, 319, 334, 1592, 1752
BssAI RCCGGY 2 cut(s) 1904, 2292
BssECI CCNNGG 4 cut(s) 51, 866, 1071, 1759
BssNAI GTATAC 1 cut(s) 1611
BssNI GRCGYC 1 cut(s) 7
BssT1I CCWWGG 2 cut(s) 1071, 1759
Bst1107I GTATAC 1 cut(s) 1611
Bst2UI CCWGG 2 cut(s) 857, 1977
Bst4CI ACNGT 6 cut(s) 59, 705, 892, 1646, 1730, 1953
Bst6I CTCTTC 3 cut(s) 512, 1599, 2438
BstACI GRCGYC 1 cut(s) 7
BstBI TTCGAA 1 cut(s) 1162
BstC8I GCNNGC 7 cut(s) 94, 224, 511, 631, 1305, 1376, 1807
BstDEI CTNAG 6 cut(s) 900, 939, 1005, 1411, 2017, 2319
BstF5I GGATG 6 cut(s) 298, 1123, 1156, 1528, 1629, 2068
BstHHI GCGC 3 cut(s) 222, 1251, 2071
BstMAI GTCTC 1 cut(s) 1955
BstMWI GCNNNNNNNGC 5 cut(s) 13, 89, 250, 842, 1806
BstNI CCWGG 2 cut(s) 857, 1977
BstNSI RCATGY 4 cut(s) 96, 554, 576, 1431
BstSCI CCNGG 3 cut(s) 740, 855, 1975
BstSFI CTRYAG 6 cut(s) 494, 561, 1092, 1265, 1372, 1642
BstSLI GKGCMC 3 cut(s) 322, 703, 2396
BstV1I GCAGC 7 cut(s) 3, 253, 1230, 1348, 1443, 2053, 2392
BstV2I GAAGAC 1 cut(s) 1491
BstX2I RGATCY 4 cut(s) 302, 1028, 1106, 2241
BstXI CCANNNNNNTGG 1 cut(s) 2295
BstYI RGATCY 4 cut(s) 302, 1028, 1106, 2241
BstZ17I GTATAC 1 cut(s) 1611
Bsu15I ATCGAT 1 cut(s) 456
BsuI GTATCC 1 cut(s) 1930
BsuRI GGCC 6 cut(s) 855, 1277, 1527, 1553, 1805, 2394
BsuTUI ATCGAT 1 cut(s) 456
BtsCI GGATG 6 cut(s) 298, 1123, 1156, 1528, 1629, 2068
BtsI GCAGTG 2 cut(s) 1821, 2191
BtsIMutI CAGTG 9 cut(s) 254, 327, 690, 701, 1251, 1599, 1651, 1821, 2191
Cac8I GCNNGC 7 cut(s) 94, 224, 511, 631, 1305, 1376, 1807
CaiI CAGNNNCTG 1 cut(s) 1580
CciI TCATGA 2 cut(s) 933, 1867
CfoI GCGC 3 cut(s) 222, 1251, 2071
Cfr10I RCCGGY 2 cut(s) 1904, 2292
Cfr13I GGNCC 3 cut(s) 1022, 2392, 2393
ClaI ATCGAT 1 cut(s) 456
Csp6I GTAC 8 cut(s) 479, 717, 884, 953, 1402, 1687, 1819, 1908
CviQI GTAC 8 cut(s) 479, 717, 884, 953, 1402, 1687, 1819, 1908
DdeI CTNAG 6 cut(s) 900, 939, 1005, 1411, 2017, 2319
DraI TTTAAA 1 cut(s) 210
EaeI YGGCCR 1 cut(s) 1551
Eam1104I CTCTTC 3 cut(s) 512, 1599, 2438
EarI CTCTTC 3 cut(s) 512, 1599, 2438
Eco130I CCWWGG 2 cut(s) 1071, 1759
Eco147I AGGCCT 2 cut(s) 855, 1277
Eco24I GRGCYC 1 cut(s) 2396
Eco47I GGWCC 1 cut(s) 1022
Eco57I CTGAAG 1 cut(s) 834
Eco88I CYCGRG 2 cut(s) 50, 2196
EcoO109I RGGNCCY 2 cut(s) 1022, 2393
EcoRII CCWGG 2 cut(s) 855, 1975
EcoT14I CCWWGG 2 cut(s) 1071, 1759
EcoT22I ATGCAT 2 cut(s) 98, 645
EcoT38I GRGCYC 1 cut(s) 2396
ErhI CCWWGG 2 cut(s) 1071, 1759
Esp3I CGTCTC 1 cut(s) 1955
FaqI GGGAC 1 cut(s) 1035
FauNDI CATATG 2 cut(s) 946, 1011
FbaI TGATCA 3 cut(s) 178, 379, 1717
FblI GTMKAC 2 cut(s) 772, 1610
Fnu4HI GCNGC 7 cut(s) 17, 242, 1219, 1337, 1432, 2067, 2406
FokI GGATG 6 cut(s) 305, 1110, 1163, 1535, 1636, 2075
FriOI GRGCYC 1 cut(s) 2396
Fsp4HI GCNGC 7 cut(s) 17, 242, 1219, 1337, 1432, 2067, 2406
FspBI CTAG 5 cut(s) 435, 1653, 1844, 2051, 2363
FspI TGCGCA 2 cut(s) 221, 1250
GlaI GCGC 3 cut(s) 221, 1250, 2070
GluI GCNGC 7 cut(s) 17, 242, 1219, 1337, 1432, 2067, 2406
GsaI CCCAGC 1 cut(s) 1830
GsuI CTGGAG 4 cut(s) 263, 1769, 1998, 2238
HaeIII GGCC 6 cut(s) 855, 1277, 1527, 1553, 1805, 2394
HapII CCGG 5 cut(s) 171, 741, 1678, 1905, 2293
HhaI GCGC 3 cut(s) 222, 1251, 2071
Hin1I GRCGYC 1 cut(s) 7
Hin6I GCGC 3 cut(s) 220, 1249, 2069
HinP1I GCGC 3 cut(s) 220, 1249, 2069
HincII GTYRAC 3 cut(s) 815, 1188, 1366
HindII GTYRAC 3 cut(s) 815, 1188, 1366
HinfI GANTC 6 cut(s) 1120, 1192, 1597, 1840, 1982, 2348
HpaII CCGG 5 cut(s) 171, 741, 1678, 1905, 2293
HphI GGTGA 3 cut(s) 185, 1639, 2031
HpyAV CCTTC 4 cut(s) 683, 898, 1288, 1692
HpyCH4III ACNGT 6 cut(s) 59, 705, 892, 1646, 1730, 1953
HpyCH4IV ACGT 2 cut(s) 697, 1964
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 89, 250, 842, 1806
HpyF3I CTNAG 6 cut(s) 900, 939, 1005, 1411, 2017, 2319
HpySE526I ACGT 2 cut(s) 697, 1964
Hsp92I GRCGYC 1 cut(s) 7
HspAI GCGC 3 cut(s) 220, 1249, 2069
KflI GGGWCCC 1 cut(s) 1022
KpnI GGTACC 2 cut(s) 956, 1911
Ksp22I TGATCA 3 cut(s) 178, 379, 1717
LmnI GCTCC 3 cut(s) 1322, 1712, 2335
Lsp1109I GCAGC 7 cut(s) 3, 253, 1230, 1348, 1443, 2053, 2392
LweI GCATC 9 cut(s) 105, 253, 394, 415, 462, 473, 536, 937, 2053
MaeI CTAG 5 cut(s) 435, 1653, 1844, 2051, 2363
MaeII ACGT 2 cut(s) 697, 1964
MaeIII GTNAC 4 cut(s) 542, 1418, 1634, 1730
MfeI CAATTG 4 cut(s) 36, 776, 1486, 2400
MflI RGATCY 4 cut(s) 302, 1028, 1106, 2241
MhlI GDGCHC 4 cut(s) 322, 703, 989, 2396
MlyI GAGTC 1 cut(s) 1201
MmeI TCCRAC 2 cut(s) 1812, 1972
Mph1103I ATGCAT 2 cut(s) 98, 645
MroXI GAANNNNTTC 3 cut(s) 972, 1158, 1385
MslI CAYNNNNRTG 3 cut(s) 91, 563, 2175
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 5 cut(s) 171, 741, 1678, 1905, 2293
MspR9I CCNGG 3 cut(s) 742, 857, 1977
MunI CAATTG 4 cut(s) 36, 776, 1486, 2400
Mva1269I GAATGC 2 cut(s) 1475, 2012
MvaI CCWGG 2 cut(s) 857, 1977
MwoI GCNNNNNNNGC 5 cut(s) 13, 89, 250, 842, 1806
NciI CCSGG 1 cut(s) 742
NdeI CATATG 2 cut(s) 946, 1011
NlaIV GGNNCC 7 cut(s) 954, 1023, 1024, 1108, 1665, 1909, 2394
NmuCI GTSAC 3 cut(s) 1418, 1634, 1730
NsbI TGCGCA 2 cut(s) 221, 1250
NsiI ATGCAT 2 cut(s) 98, 645
NspI RCATGY 4 cut(s) 96, 554, 576, 1431
NspV TTCGAA 1 cut(s) 1162
OliI CACNNNNGTG 1 cut(s) 563
PaeI GCATGC 1 cut(s) 96
PaeR7I CTCGAG 1 cut(s) 2196
PagI TCATGA 2 cut(s) 933, 1867
PceI AGGCCT 2 cut(s) 855, 1277
PciI ACATGT 1 cut(s) 572
PctI GAATGC 2 cut(s) 1475, 2012
PdmI GAANNNNTTC 3 cut(s) 972, 1158, 1385
PfeI GAWTC 5 cut(s) 1120, 1597, 1840, 1982, 2348
PflMI CCANNNNNTGG 1 cut(s) 329
PkrI GCNGC 7 cut(s) 18, 243, 1220, 1338, 1433, 2068, 2407
PleI GAGTC 1 cut(s) 1200
PpsI GAGTC 1 cut(s) 1200
PpuMI RGGWCCY 1 cut(s) 1022
PscI ACATGT 1 cut(s) 572
PshBI ATTAAT 2 cut(s) 261, 1083
PsiI TTATAA 1 cut(s) 354
Psp5II RGGWCCY 1 cut(s) 1022
Psp6I CCWGG 2 cut(s) 855, 1975
PspFI CCCAGC 1 cut(s) 1826
PspGI CCWGG 2 cut(s) 855, 1975
PspN4I GGNNCC 7 cut(s) 954, 1023, 1024, 1108, 1665, 1909, 2394
PspOMI GGGCCC 1 cut(s) 2392
PspPI GGNCC 3 cut(s) 1022, 2392, 2393
PspPPI RGGWCCY 1 cut(s) 1022
PsrI GAACNNNNNNTAC 2 cut(s) 1248, 1280
PstI CTGCAG 1 cut(s) 1376
PstNI CAGNNNCTG 1 cut(s) 1580
PsuI RGATCY 4 cut(s) 302, 1028, 1106, 2241
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 8 cut(s) 480, 718, 885, 954, 1403, 1688, 1820, 1909
RsaNI GTAC 8 cut(s) 479, 717, 884, 953, 1402, 1687, 1819, 1908
RseI CAYNNNNRTG 3 cut(s) 91, 563, 2175
SatI GCNGC 7 cut(s) 17, 242, 1219, 1337, 1432, 2067, 2406
Sau96I GGNCC 3 cut(s) 1022, 2392, 2393
ScaI AGTACT 1 cut(s) 718
SchI GAGTC 1 cut(s) 1201
ScrFI CCNGG 3 cut(s) 742, 857, 1977
SduI GDGCHC 4 cut(s) 322, 703, 989, 2396
SfaNI GCATC 9 cut(s) 105, 253, 394, 415, 462, 473, 536, 937, 2053
SfcI CTRYAG 6 cut(s) 494, 561, 1092, 1265, 1372, 1642
Sfr274I CTCGAG 1 cut(s) 2196
SfuI TTCGAA 1 cut(s) 1162
SinI GGWCC 1 cut(s) 1022
SlaI CTCGAG 1 cut(s) 2196
SmiMI CAYNNNNRTG 3 cut(s) 91, 563, 2175
SmlI CTYRAG 2 cut(s) 1271, 2196
SmoI CTYRAG 2 cut(s) 1271, 2196
SpeI ACTAGT 1 cut(s) 434
SphI GCATGC 1 cut(s) 96
SseBI AGGCCT 2 cut(s) 855, 1277
SsiI CCGC 1 cut(s) 128
SspI AATATT 1 cut(s) 972
SspMI CTAG 5 cut(s) 435, 1653, 1844, 2051, 2363
StuI AGGCCT 2 cut(s) 855, 1277
StyD4I CCNGG 3 cut(s) 740, 855, 1975
StyI CCWWGG 2 cut(s) 1071, 1759
TaaI ACNGT 6 cut(s) 59, 705, 892, 1646, 1730, 1953
TaiI ACGT 2 cut(s) 700, 1967
TaqI TCGA 6 cut(s) 442, 456, 1162, 1788, 1969, 2197
TatI WGTACW 3 cut(s) 716, 883, 1686
TfiI GAWTC 5 cut(s) 1120, 1597, 1840, 1982, 2348
TscAI CASTG 9 cut(s) 254, 334, 697, 708, 1258, 1599, 1651, 1828, 2191
TseFI GTSAC 3 cut(s) 1418, 1634, 1730
TseI GCWGC 7 cut(s) 16, 241, 1218, 1336, 1431, 2066, 2405
Tsp45I GTSAC 3 cut(s) 1418, 1634, 1730
TspGWI ACGGA 3 cut(s) 379, 637, 2156
TspRI CASTG 9 cut(s) 254, 334, 697, 708, 1258, 1599, 1651, 1828, 2191
Van91I CCANNNNNTGG 1 cut(s) 329
VneI GTGCAC 2 cut(s) 318, 699
VpaK11BI GGWCC 1 cut(s) 1022
VspI ATTAAT 2 cut(s) 261, 1083
XapI RAATTY 5 cut(s) 228, 385, 1384, 1898, 2201
XceI RCATGY 4 cut(s) 96, 554, 576, 1431
XhoI CTCGAG 1 cut(s) 2196
XmiI GTMKAC 2 cut(s) 772, 1610
XmnI GAANNNNTTC 3 cut(s) 972, 1158, 1385
XspI CTAG 5 cut(s) 435, 1653, 1844, 2051, 2363
ZrmI AGTACT 1 cut(s) 718
Zsp2I ATGCAT 2 cut(s) 98, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.