pycom07g18980

ARF guanine-nucleotide exchange factor GNOM-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001807
Physical Location & Seq
Reverse (-)
21328521 .. 21332017
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18980.2

Sequence Viewer

Length: 3345 bp
ATGGGGCGTCTGAAGCTGCAAAGTGGAATCCAGGAAATTGAGGAAGAACATGGGGAATGTGATGCAACGCATTCTAACCATGCTACTTTAGCATGCATCATTAATTCAGAAATAGGCACTGTATTAGTGGTTATGAGGAGGAATGTAAGATGGGGAGGACGCTATGTGTCTGGTGATGATCAACTGGAACACCCTCTAATCCAATCTTTAAAGGCGTTACGCAAGCAAATCTTTTCATGGAAGCACCATATGCATTCCATCAATCCTTCTGCGTATCTCCGGCCATTTTTGGATGTGATTAGGTCGGATGAAACTGGTGCACCAATTACTGGTGTGGCTTTGTCTTCTGTTTACAATATCTTAACACTTGATGTAATCAATCAAAATTCCTTATATGTTGAAGATGCCATGCACTTATTAGTTGATGCAGTCACTGGCTGCCGATTTGAGGTGACTGATCCTGCATCAGAAGAAGTGGTATTGATGAAGATACTGCAGGTTCTTCTAGCTTGCATGAAGAGTAAAGCATCTGTTATTTTGACCAATCAGCATGTTTGCATTATAGTGAACACATGTTTCCGTATTGTCCATCAAGCGGGAACAAAAGGTGAGTTGTTGCAGCGGATAGCTCGCCACACAATTCATGAACTTATTAGATGTATTTTCTCACACCTTCCAGATGTTCACCATGCTGAACATGCATTGCCAAATGGAAGCAATACCATCAATGGAGAGATTGCAGGGCAAAATAATGAGCATGCTTCTGGGAGTAGACAATTGGAGAATGATAACATGATTTCTCAGTTTGATAGTCATCTATTATCTACAAATCCTGCTTTGCATGCTTCCTCAGGTCTTGTAGAATCTGGGATTGATGAAAAGACAGCTGGTGGTTCTAGCAGGAAGGAGGCTGATCAATATGACGTGCAACTCATGGCTGAGCCATTTGGGATTCCCTGCATGGTGGAAATATTTGACTTCCTGTGTTCATTGTTAAATGTGGTTGAGCATATAGGAATGGGTCCCAGGTATAATACCATAGCTTTTGACGAAGATGTCCCCCTTTTTGCACTGGGTTTGGTTAATTGCGCTATAGAGTTAGGCGGGTCCTCCATTCGGCATCACACCAAGTTATTAAGTTTGGTTCAGGATGAATTGTTTCAAAATTTGATGCAGTTTGGCCTATCAACAAGTCCACTTATTCTTTCAATGGTATGCAGCATTGTCCTCAATCTGTATCACCATCTCCGCACTGAACTTAAGCTACAGCTTGAGGCTTTCTTTTCATGTGTGATATTGAGGCTTGTGCAAAGCAGGTATGGGGCTTCTTATCAGCAGCAGGAGGTTGCCATGGAGTCTGTTGTGGACTTCTGCCGGCAGAAAACTTTCATGGTGGAGATGTACGCGAACTTAGATTGTGACATAACTTGCAATAATGTCTTTGAAGAGCTTGCTAAAATGTTGTCAAAGAGTGCATTCCCTGTTAATTTGCTTTTGTCTTCAATTCACATTCTTGCTTTGGATGGTCTTATTGCTATTATTCAGGGAATGGCAGAGAGGGCAGGGAATGGCTCAGTTAGTGCTGTTCAAGCTCCCCCTATGAATCTTGATGAGTATACTCCATTCTGGTTGATGAAATGTGAAAACTACAGTGATCCGACTCATTGGGTTCCATTTGTTCGCCGAAGGAAGCACATAAAGAGAGGACTAATGATTGGAGCTGATCATTTTAACCATGACCCAAAGAAAGGGCTGGAGTTCCTCCAAGGAACCCATCTCTTGCCTGACAAACTTGATCCTGAAAGTGTGGCCTGTTTTTTCAGGTACACTGCTGGGTTGGACAAGAATCTTGTTGGGGATTTCCTGGGAAACCATGATGAGTTTTGTATTCAGGTTCTTAATAAATTTGCTGGTACCTTTGATTTCCAAGACATGCATTTGGATACTGCACTGCGACTGTTTCTGGAGACATTTCGATTGCCTGGAGAATCACAAAAAATACAAAGGGTGCTTGAAGCATTCTCAGAGAGATATTATAAGCAATCACCACAAATTCTGGCTAACAAGGATTCTGCTCTCCTGTTATCATATTCAATTATATTGCTCAACACGGACCAGCACAATGTTCAGGTGAAGAAGAAGATGACAGAGGAGGATTTCATTCGGAATAATCGACACATCAATGGAGGTGATGATTTACCTCGAGAATTCCTTTCAGAGCTTTACCACTCAATATGCAAGAATGAGATCCGCACAACTCCAGAACAAGGTACTGGTTATCCTGAAATGAACCCAAGCCGTTGGATTGATTTAATACACAAATCTAAGAAGAATGCTCCATTCATTGAATCCGATACCAGAGCCTACCTTGACCAGGATATGTTTGCTATAATGTCAGGCCCCACAATTGCTGCCATTTCTGTGGTATTTGATCATGCAGAACATGAAGAGATATACCAAAAATGCATTGATGGATTTTTATCTGTTGCAAAGATTGCAGCATGCTATCATTTTGAAGATGTGCTCGACGATCTGATTGTGTCTCTTTGTAAGTTTACAACCCTTTTAAACTCATCTTATGAGGAACCTGTTTTAGCCTTTGGTGATGACCCAAAAGCTAGAATGTCAACAGTGACAGTTTTCACGATTGCCAATACATATGGTGATCACATTCGCACAGGCTGGAGAAATGTTCTTGACTGTATCTTAAGATTACACAAACTTGGTCTTCTGTCGGCTTTTGCGGCCAGTGAAGCAGCTGGTGATTCTGAGTTTTCTGCTGACACAGGTCATGGCAAGGCCATAACCAATTCTCTGTCATCCGTTCCTATGCCCCCTCTGAGTACTCCTAGGAAATCCTCTGGATTGATGGGCCGGTTTACTCAGCTATTATCTCTTGACACTGAGGAGCCGAGATCACAGCCTACTGAAGAAGAACTTGTGGTTCATCAGCGCACCCTTCAGACAATTCAAAAGTGCCACATTGATAGCATATTTTCGGAGAGCAAGTTTCTGCACGCTAAATCCCTGTTACAGCTTGCACGGGCACTCATCTGGGCTGGAGGACGACCCCAGAAAGGGAGCAGCTCACCTGAGGATGAAGATACTGCAGTTTTCTGTTTGGAGTTGCTGATAGCTGTTACCCTAAATAACAGGGATAGGATTATGCTTCTTTGGCAGATTGTGTATGAGCACATATCGAACATTGTTCAGTCTACCGTGATGCCTTGTGCCCTGGTAGAGAAGGCTGTTTTTGGACTTCTTCGGATTTGCCAGCGGCTGCTTCCCTATAAAGAGGACCTTGCTGATGATCTTTTGCACTATGATCATTGCAACTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Sec7 Sec7_N

Protein Analysis

1115

Amino Acids

125.04

Weight (kDa)

5.64

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2037
AasI GACNNNNNNGTC 1 cut(s) 1055
Acc36I ACCTGC 2 cut(s) 487, 1305
Acc65I GGTACC 1 cut(s) 1913
AccB1I GGYRCC 1 cut(s) 1913
AccB7I CCANNNNNTGG 1 cut(s) 329
AccI GTMKAC 3 cut(s) 772, 1616, 3215
AccII CGCG 1 cut(s) 1406
AciI CCGC 7 cut(s) 596, 622, 1104, 1249, 2251, 2744, 3277
AclWI GGATC 4 cut(s) 452, 1649, 1790, 2242
AcoI YGGCCR 2 cut(s) 281, 2745
AcsI RAATTY 5 cut(s) 385, 1165, 1904, 2052, 2207
AcuI CTGAAG 3 cut(s) 32, 2945, 2949
AcyI GRCGYC 1 cut(s) 7
AfaI GTAC 5 cut(s) 1403, 1826, 1915, 2272, 2845
AfiI CCNNNNNNNGG 9 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374, 3077, 3078
AflII CTTAAG 2 cut(s) 1259, 2707
AflIII ACRYGT 1 cut(s) 572
AjiI CACGTC 1 cut(s) 925
AjnI CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3234
Alw21I GWGCWC 3 cut(s) 322, 2526, 3195
Alw26I GTCTC 2 cut(s) 1961, 2547
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 4 cut(s) 452, 1649, 1790, 2242
AlwNI CAGNNNCTG 2 cut(s) 434, 3280
Ama87I CYCGRG 1 cut(s) 2202
AoxI GGCC 7 cut(s) 281, 1180, 1809, 2398, 2745, 2799, 2872
ApaLI GTGCAC 1 cut(s) 318
ApoI RAATTY 5 cut(s) 385, 1165, 1904, 2052, 2207
AseI ATTAAT 1 cut(s) 102
Asp700I GAANNNNTTC 2 cut(s) 1158, 1385
Asp718I GGTACC 1 cut(s) 1913
AspA2I CCTAGG 1 cut(s) 2849
AspLEI GCGC 2 cut(s) 1091, 2955
AspS9I GGNCC 6 cut(s) 1022, 1107, 2113, 2399, 2872, 3298
AvaI CYCGRG 1 cut(s) 2202
AvaII GGWCC 4 cut(s) 1022, 1107, 2113, 3298
AvrII CCTAGG 1 cut(s) 2849
AxyI CCTNAGG 2 cut(s) 850, 3093
BaeGI GKGCMC 3 cut(s) 322, 3049, 3235
BaeI ACNNNNGTAYC 2 cut(s) 3096, 3129
BanI GGYRCC 1 cut(s) 1913
BbsI GAAGAC 3 cut(s) 336, 1491, 2720
Bbv12I GWGCWC 3 cut(s) 322, 2526, 3195
BccI CCATC 9 cut(s) 144, 266, 597, 731, 1251, 1517, 1782, 2465, 2863
BceAI ACGGC 1 cut(s) 2283
BciT130I CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
BciVI GTATCC 1 cut(s) 1936
BclI TGATCA 6 cut(s) 178, 913, 1723, 2431, 2665, 3325
BcoDI GTCTC 2 cut(s) 1961, 2547
BfaI CTAG 4 cut(s) 506, 897, 2619, 2850
BfmI CTRYAG 5 cut(s) 494, 1092, 1265, 1648, 3108
BfrI CTTAAG 2 cut(s) 1259, 2707
BfuAI ACCTGC 2 cut(s) 487, 1305
BfuI GTATCC 1 cut(s) 1936
BlnI CCTAGG 1 cut(s) 2849
BlpI GCTNAGC 1 cut(s) 939
BmcAI AGTACT 1 cut(s) 2845
Bme1390I CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
Bme18I GGWCC 4 cut(s) 1022, 1107, 2113, 3298
BmeT110I CYCGRG 1 cut(s) 2202
BmgBI CACGTC 1 cut(s) 925
BmgT120I GGNCC 6 cut(s) 1022, 1107, 2113, 2399, 2872, 3298
BmiI GGNNCC 9 cut(s) 1023, 1024, 1108, 1671, 1771, 1915, 2401, 2586, 2910
BmrFI CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
BmrI ACTGGG 1 cut(s) 1082
BmsI GCATC 9 cut(s) 52, 105, 394, 415, 473, 536, 1129, 1161, 3213
BmuI ACTGGG 1 cut(s) 1082
BoxI GACNNNNGTC 1 cut(s) 2787
BpiI GAAGAC 3 cut(s) 336, 1491, 2720
BpmI CTGGAG 6 cut(s) 1775, 1985, 2004, 2244, 2704, 3081
Bpu1102I GCTNAGC 1 cut(s) 939
BpuEI CTTGAG 1 cut(s) 1292
BsaBI GATNNNNATC 2 cut(s) 813, 2479
BsaHI GRCGYC 1 cut(s) 7
BsaJI CCNNGG 6 cut(s) 1025, 1350, 1765, 1864, 2849, 3234
BsaXI ACNNNNNCTCC 2 cut(s) 2993, 3023
Bsc4I CCNNNNNNNGG 9 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374, 3077, 3078
Bse118I RCCGGY 2 cut(s) 1374, 2874
Bse1I ACTGG 7 cut(s) 189, 319, 334, 439, 1077, 2278, 2748
Bse21I CCTNAGG 2 cut(s) 850, 3093
Bse3DI GCAATG 2 cut(s) 701, 3328
Bse8I GATNNNNATC 2 cut(s) 813, 2479
BseBI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
BseDI CCNNGG 6 cut(s) 1025, 1350, 1765, 1864, 2849, 3234
BseGI GGATG 6 cut(s) 298, 313, 1156, 1528, 2819, 3103
BseJI GATNNNNATC 2 cut(s) 813, 2479
BseLI CCNNNNNNNGG 9 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374, 3077, 3078
BseMI GCAATG 2 cut(s) 701, 3328
BseNI ACTGG 7 cut(s) 189, 319, 334, 439, 1077, 2278, 2748
BseRI GAGGAG 3 cut(s) 151, 2165, 2921
BseSI GKGCMC 3 cut(s) 322, 3049, 3235
BseYI CCCAGC 1 cut(s) 1832
BsgI GTGCAG 2 cut(s) 1932, 2999
Bsh1236I CGCG 1 cut(s) 1406
BshFI GGCC 7 cut(s) 283, 1182, 1811, 2400, 2747, 2801, 2874
BshNI GGYRCC 1 cut(s) 1913
BsiHKAI GWGCWC 3 cut(s) 322, 2526, 3195
BsiHKCI CYCGRG 1 cut(s) 2202
BsiSI CCGG 3 cut(s) 280, 1375, 2875
BslFI GGGAC 2 cut(s) 1008, 1043
BslI CCNNNNNNNGG 9 cut(s) 329, 448, 595, 1116, 1748, 2267, 2374, 3077, 3078
BsmAI GTCTC 2 cut(s) 1961, 2547
BsmFI GGGAC 2 cut(s) 1008, 1043
BsmI GAATGC 5 cut(s) 70, 253, 1475, 2018, 2338
BsnI GGCC 7 cut(s) 283, 1182, 1811, 2400, 2747, 2801, 2874
BsoBI CYCGRG 1 cut(s) 2202
Bsp1286I GDGCHC 5 cut(s) 322, 2526, 3049, 3195, 3235
Bsp1720I GCTNAGC 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 1350
BspACI CCGC 7 cut(s) 596, 622, 1104, 1249, 2251, 2744, 3277
BspANI GGCC 7 cut(s) 283, 1182, 1811, 2400, 2747, 2801, 2874
BspFNI CGCG 1 cut(s) 1406
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 9 cut(s) 1023, 1024, 1108, 1671, 1771, 1915, 2401, 2586, 2910
BspMAI CTGCAG 2 cut(s) 498, 3112
BspMI ACCTGC 2 cut(s) 487, 1305
BspPI GGATC 4 cut(s) 452, 1649, 1790, 2242
BspQI GCTCTTC 1 cut(s) 1440
BspT107I GGYRCC 1 cut(s) 1913
BspTI CTTAAG 2 cut(s) 1259, 2707
BsrDI GCAATG 2 cut(s) 701, 3328
BsrFI RCCGGY 2 cut(s) 1374, 2874
BsrI ACTGG 7 cut(s) 189, 319, 334, 439, 1077, 2278, 2748
BssAI RCCGGY 2 cut(s) 1374, 2874
BssECI CCNNGG 6 cut(s) 1025, 1350, 1765, 1864, 2849, 3234
BssNAI GTATAC 1 cut(s) 1617
BssNI GRCGYC 1 cut(s) 7
BssT1I CCWWGG 3 cut(s) 1350, 1765, 2849
Bst1107I GTATAC 1 cut(s) 1617
Bst2UI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
Bst4CI ACNGT 7 cut(s) 121, 1652, 1959, 2632, 2638, 2702, 3220
Bst6I CTCTTC 3 cut(s) 512, 1440, 2442
BstACI GRCGYC 1 cut(s) 7
BstAFI CTTAAG 2 cut(s) 1259, 2707
BstAPI GCANNNNNTGC 2 cut(s) 250, 2495
BstDSI CCRYGG 1 cut(s) 1350
BstENI CCTNNNNNAGG 1 cut(s) 2372
BstF5I GGATG 6 cut(s) 298, 313, 1156, 1528, 2819, 3103
BstFNI CGCG 1 cut(s) 1406
BstHHI GCGC 2 cut(s) 1091, 2955
BstMAI GTCTC 2 cut(s) 1961, 2547
BstNI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
BstNSI RCATGY 8 cut(s) 96, 554, 576, 701, 761, 845, 1936, 2505
BstPAI GACNNNNGTC 1 cut(s) 2787
BstSCI CCNGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3234
BstSFI CTRYAG 5 cut(s) 494, 1092, 1265, 1648, 3108
BstSLI GKGCMC 3 cut(s) 322, 3049, 3235
BstUI CGCG 1 cut(s) 1406
BstV2I GAAGAC 3 cut(s) 336, 1491, 2720
BstX2I RGATCY 1 cut(s) 2247
BstXI CCANNNNNNTGG 2 cut(s) 2301, 2422
BstYI RGATCY 1 cut(s) 2247
BstZ17I GTATAC 1 cut(s) 1617
Bsu36I CCTNAGG 2 cut(s) 850, 3093
BsuI GTATCC 1 cut(s) 1936
BsuRI GGCC 7 cut(s) 283, 1182, 1811, 2400, 2747, 2801, 2874
BtgI CCRYGG 1 cut(s) 1350
BtrI CACGTC 1 cut(s) 925
BtsCI GGATG 6 cut(s) 298, 313, 1156, 1528, 2819, 3103
BtsI GCAGTG 2 cut(s) 1827, 1949
BveI ACCTGC 2 cut(s) 487, 1305
CaiI CAGNNNCTG 2 cut(s) 434, 3280
CciI TCATGA 1 cut(s) 643
CfoI GCGC 2 cut(s) 1091, 2955
Cfr10I RCCGGY 2 cut(s) 1374, 2874
Cfr13I GGNCC 6 cut(s) 1022, 1107, 2113, 2399, 2872, 3298
CseI GACGC 1 cut(s) 168
Csp6I GTAC 5 cut(s) 1402, 1825, 1914, 2271, 2844
CviQI GTAC 5 cut(s) 1402, 1825, 1914, 2271, 2844
DraI TTTAAA 2 cut(s) 210, 2568
DrdI GACNNNNNNGTC 1 cut(s) 1055
DseDI GACNNNNNNGTC 1 cut(s) 1055
EaeI YGGCCR 2 cut(s) 281, 2745
Eam1104I CTCTTC 3 cut(s) 512, 1440, 2442
EarI CTCTTC 3 cut(s) 512, 1440, 2442
Eco130I CCWWGG 3 cut(s) 1350, 1765, 2849
Eco47I GGWCC 4 cut(s) 1022, 1107, 2113, 3298
Eco57I CTGAAG 3 cut(s) 32, 2945, 2949
Eco81I CCTNAGG 2 cut(s) 850, 3093
Eco88I CYCGRG 1 cut(s) 2202
EcoNI CCTNNNNNAGG 1 cut(s) 2372
EcoO109I RGGNCCY 4 cut(s) 1022, 1107, 2399, 3298
EcoRI GAATTC 1 cut(s) 2207
EcoRII CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3234
EcoT14I CCWWGG 3 cut(s) 1350, 1765, 2849
EcoT22I ATGCAT 5 cut(s) 98, 255, 703, 1938, 2468
ErhI CCWWGG 3 cut(s) 1350, 1765, 2849
FalI AAGNNNNNCTT 6 cut(s) 215, 247, 2922, 2954, 3285, 3317
FaqI GGGAC 2 cut(s) 1008, 1043
FauI CCCGC 2 cut(s) 589, 1097
FauNDI CATATG 2 cut(s) 249, 2659
FbaI TGATCA 6 cut(s) 178, 913, 1723, 2431, 2665, 3325
FblI GTMKAC 3 cut(s) 772, 1616, 3215
FokI GGATG 6 cut(s) 305, 320, 1163, 1535, 2806, 3110
FspBI CTAG 4 cut(s) 506, 897, 2619, 2850
GlaI GCGC 2 cut(s) 1090, 2954
GsaI CCCAGC 1 cut(s) 1836
GsuI CTGGAG 6 cut(s) 1775, 1985, 2004, 2244, 2704, 3081
HaeIII GGCC 7 cut(s) 283, 1182, 1811, 2400, 2747, 2801, 2874
HapII CCGG 3 cut(s) 280, 1375, 2875
HgaI GACGC 1 cut(s) 168
HhaI GCGC 2 cut(s) 1091, 2955
Hin1I GRCGYC 1 cut(s) 7
Hin6I GCGC 2 cut(s) 1089, 2953
HinP1I GCGC 2 cut(s) 1089, 2953
HincII GTYRAC 1 cut(s) 2628
HindII GTYRAC 1 cut(s) 2628
HpaII CCGG 3 cut(s) 280, 1375, 2875
Hpy99I CGWCG 1 cut(s) 2531
HpyAV CCTTC 6 cut(s) 276, 683, 898, 1680, 2969, 3238
HpyCH4III ACNGT 7 cut(s) 121, 1652, 1959, 2632, 2638, 2702, 3220
HpyCH4IV ACGT 1 cut(s) 924
HpySE526I ACGT 1 cut(s) 924
Hsp92I GRCGYC 1 cut(s) 7
HspAI GCGC 2 cut(s) 1089, 2953
KflI GGGWCCC 1 cut(s) 1022
KpnI GGTACC 1 cut(s) 1917
KroI GCCGGC 1 cut(s) 1374
KroNI GCCGGC 1 cut(s) 1376
Ksp22I TGATCA 6 cut(s) 178, 913, 1723, 2431, 2665, 3325
LguI GCTCTTC 1 cut(s) 1440
LmnI GCTCC 5 cut(s) 1597, 1718, 2341, 2908, 3081
LweI GCATC 9 cut(s) 52, 105, 394, 415, 473, 536, 1129, 1161, 3213
MaeI CTAG 4 cut(s) 506, 897, 2619, 2850
MaeII ACGT 1 cut(s) 924
MaeIII GTNAC 7 cut(s) 216, 430, 451, 1418, 2632, 3030, 3139
MfeI CAATTG 2 cut(s) 776, 2406
MflI RGATCY 1 cut(s) 2247
MhlI GDGCHC 5 cut(s) 322, 2526, 3049, 3195, 3235
MlyI GAGTC 2 cut(s) 1364, 1654
MmeI TCCRAC 4 cut(s) 285, 1682, 1818, 2282
Mph1103I ATGCAT 5 cut(s) 98, 255, 703, 1938, 2468
MroNI GCCGGC 1 cut(s) 1374
MroXI GAANNNNTTC 2 cut(s) 1158, 1385
MslI CAYNNNNRTG 3 cut(s) 563, 2181, 2420
MspA1I CMGCKG 4 cut(s) 622, 887, 2759, 3277
MspCI CTTAAG 2 cut(s) 1259, 2707
MspI CCGG 3 cut(s) 280, 1375, 2875
MspR9I CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
MunI CAATTG 2 cut(s) 776, 2406
Mva1269I GAATGC 5 cut(s) 70, 253, 1475, 2018, 2338
MvaI CCWGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
MvnI CGCG 1 cut(s) 1406
NaeI GCCGGC 1 cut(s) 1376
NcoI CCATGG 1 cut(s) 1350
NdeI CATATG 2 cut(s) 249, 2659
NgoMIV GCCGGC 1 cut(s) 1374
NlaIV GGNNCC 9 cut(s) 1023, 1024, 1108, 1671, 1771, 1915, 2401, 2586, 2910
NmeAIII GCCGAG 1 cut(s) 2937
NmuCI GTSAC 4 cut(s) 430, 451, 1418, 2632
NsiI ATGCAT 5 cut(s) 98, 255, 703, 1938, 2468
NspI RCATGY 8 cut(s) 96, 554, 576, 701, 761, 845, 1936, 2505
PaeI GCATGC 4 cut(s) 96, 761, 845, 2505
PaeR7I CTCGAG 1 cut(s) 2202
PagI TCATGA 1 cut(s) 643
PciI ACATGT 1 cut(s) 572
PciSI GCTCTTC 1 cut(s) 1440
PcsI WCGNNNNNNNCGW 1 cut(s) 2170
PctI GAATGC 5 cut(s) 70, 253, 1475, 2018, 2338
PdiI GCCGGC 1 cut(s) 1376
PdmI GAANNNNTTC 2 cut(s) 1158, 1385
PfeI GAWTC 9 cut(s) 27, 863, 952, 1603, 1846, 1988, 2069, 2348, 2765
PflMI CCANNNNNTGG 1 cut(s) 329
PfoI TCCNGGA 1 cut(s) 30
PleI GAGTC 2 cut(s) 1363, 1654
PpsI GAGTC 2 cut(s) 1363, 1654
PpuMI RGGWCCY 3 cut(s) 1022, 1107, 3298
PscI ACATGT 1 cut(s) 572
PshAI GACNNNNGTC 1 cut(s) 2787
PshBI ATTAAT 1 cut(s) 102
PsiI TTATAA 1 cut(s) 2037
Psp5II RGGWCCY 3 cut(s) 1022, 1107, 3298
Psp6I CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3234
PspFI CCCAGC 1 cut(s) 1832
PspGI CCWGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3234
PspN4I GGNNCC 9 cut(s) 1023, 1024, 1108, 1671, 1771, 1915, 2401, 2586, 2910
PspPI GGNCC 6 cut(s) 1022, 1107, 2113, 2399, 2872, 3298
PspPPI RGGWCCY 3 cut(s) 1022, 1107, 3298
PsrI GAACNNNNNNTAC 2 cut(s) 1248, 1280
PstI CTGCAG 2 cut(s) 498, 3112
PstNI CAGNNNCTG 2 cut(s) 434, 3280
PsuI RGATCY 1 cut(s) 2247
PvuII CAGCTG 2 cut(s) 887, 2759
RsaI GTAC 5 cut(s) 1403, 1826, 1915, 2272, 2845
RsaNI GTAC 5 cut(s) 1402, 1825, 1914, 2271, 2844
RseI CAYNNNNRTG 3 cut(s) 563, 2181, 2420
SapI GCTCTTC 1 cut(s) 1440
Sau96I GGNCC 6 cut(s) 1022, 1107, 2113, 2399, 2872, 3298
ScaI AGTACT 1 cut(s) 2845
SchI GAGTC 2 cut(s) 1364, 1654
ScrFI CCNGG 6 cut(s) 32, 1027, 1865, 1983, 2375, 3236
SduI GDGCHC 5 cut(s) 322, 2526, 3049, 3195, 3235
SfaNI GCATC 9 cut(s) 52, 105, 394, 415, 473, 536, 1129, 1161, 3213
SfcI CTRYAG 5 cut(s) 494, 1092, 1265, 1648, 3108
Sfr274I CTCGAG 1 cut(s) 2202
SinI GGWCC 4 cut(s) 1022, 1107, 2113, 3298
SlaI CTCGAG 1 cut(s) 2202
SmiMI CAYNNNNRTG 3 cut(s) 563, 2181, 2420
SmlI CTYRAG 4 cut(s) 1259, 1271, 2202, 2707
SmoI CTYRAG 4 cut(s) 1259, 1271, 2202, 2707
SphI GCATGC 4 cut(s) 96, 761, 845, 2505
SsiI CCGC 7 cut(s) 596, 622, 1104, 1249, 2251, 2744, 3277
SspI AATATT 1 cut(s) 972
SspMI CTAG 4 cut(s) 506, 897, 2619, 2850
StyD4I CCNGG 6 cut(s) 30, 1025, 1863, 1981, 2373, 3234
StyI CCWWGG 3 cut(s) 1350, 1765, 2849
TaaI ACNGT 7 cut(s) 121, 1652, 1959, 2632, 2638, 2702, 3220
TaiI ACGT 1 cut(s) 927
TaqI TCGA 5 cut(s) 1975, 2173, 2203, 2526, 3200
TatI WGTACW 1 cut(s) 2843
TauI GCSGC 2 cut(s) 2747, 3280
TfiI GAWTC 9 cut(s) 27, 863, 952, 1603, 1846, 1988, 2069, 2348, 2765
TseFI GTSAC 4 cut(s) 430, 451, 1418, 2632
Tsp45I GTSAC 4 cut(s) 430, 451, 1418, 2632
TspGWI ACGGA 3 cut(s) 569, 2126, 2812
Van91I CCANNNNNTGG 1 cut(s) 329
Vha464I CTTAAG 2 cut(s) 1259, 2707
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 4 cut(s) 1022, 1107, 2113, 3298
VspI ATTAAT 1 cut(s) 102
XagI CCTNNNNNAGG 1 cut(s) 2372
XapI RAATTY 5 cut(s) 385, 1165, 1904, 2052, 2207
XceI RCATGY 8 cut(s) 96, 554, 576, 701, 761, 845, 1936, 2505
XhoI CTCGAG 1 cut(s) 2202
XmaJI CCTAGG 1 cut(s) 2849
XmiI GTMKAC 3 cut(s) 772, 1616, 3215
XmnI GAANNNNTTC 2 cut(s) 1158, 1385
XspI CTAG 4 cut(s) 506, 897, 2619, 2850
ZrmI AGTACT 1 cut(s) 2845
Zsp2I ATGCAT 5 cut(s) 98, 255, 703, 1938, 2468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.