MD01G1145400.v1.1

Ribosomal protein L35Ae

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003771
Physical Location & Seq
Forward (+)
25540245 .. 25542633
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1145400.v1.1.491

Sequence Viewer

Length: 315 bp
ATGGTGAAGGAACGCCAAGGAGAGCGAATTAGACTGTACGTCAGGGGCACCGTCCTCGGATACAAGAGGTCTAAGTCGAACCAGTACCCGAACACCTCTCTGATTCAGATCGAGGGAGTGAACACCAAAGAGGAGGTTGCGTGGTATGCCGGGAAGCGGTTGGCCTACATCTACAAGGCCAAGGTGAAGACCAATGGCAGCCACTACCGCTGCATTTGGGGCAAGGTTTCCCGGCCTCATGGTAACAGCGGCGTAGTTCGTGCTAAGTTCACTTCCAATTTGCCCCCCAAGTCCATGGTATTGGTGATCGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L35Ae

Protein Analysis

105

Amino Acids

11.83

Weight (kDa)

10.39

Isoelectric Point (pI)

27.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35Ae PF01247 12 - 99 5e-41 Ribosomal protein L35Ae
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccII CGCG 1 cut(s) 311
AciI CCGC 3 cut(s) 157, 208, 249
AfaI GTAC 2 cut(s) 38, 86
AfiI CCNNNNNNNGG 1 cut(s) 156
AhdI GACNNNNNGTC 1 cut(s) 38
AoxI GGCC 3 cut(s) 162, 177, 233
ApeKI GCWGC 2 cut(s) 198, 210
AsuC2I CCSGG 2 cut(s) 151, 232
AsuHPI GGTGA 2 cut(s) 16, 196
BaeGI GKGCMC 1 cut(s) 50
BanI GGYRCC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 197, 210
BcgI CGANNNNNNTGC 2 cut(s) 37, 71
BciVI GTATCC 1 cut(s) 53
BcnI CCSGG 2 cut(s) 151, 232
BfuI GTATCC 1 cut(s) 53
BisI GCNGC 3 cut(s) 199, 211, 250
BlsI GCNGC 3 cut(s) 200, 212, 251
Bme1390I CCNGG 2 cut(s) 151, 232
BmeRI GACNNNNNGTC 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 2 cut(s) 151, 232
BpiI GAAGAC 1 cut(s) 194
BpuMI CCSGG 2 cut(s) 151, 232
BsaBI GATNNNNATC 1 cut(s) 107
BsaJI CCNNGG 4 cut(s) 16, 55, 180, 294
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 82
Bse8I GATNNNNATC 1 cut(s) 107
BseDI CCNNGG 4 cut(s) 16, 55, 180, 294
BseJI GATNNNNATC 1 cut(s) 107
BseLI CCNNNNNNNGG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 2 cut(s) 197, 210
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 3 cut(s) 164, 179, 235
BshNI GGYRCC 1 cut(s) 47
BsiSI CCGG 2 cut(s) 150, 232
BslI CCNNNNNNNGG 1 cut(s) 156
BsnI GGCC 3 cut(s) 164, 179, 235
Bsp1286I GDGCHC 1 cut(s) 50
Bsp143I GATC 2 cut(s) 108, 306
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 3 cut(s) 157, 208, 249
BspANI GGCC 3 cut(s) 164, 179, 235
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 47
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 4 cut(s) 16, 55, 180, 294
BssMI GATC 2 cut(s) 108, 306
BssT1I CCWWGG 3 cut(s) 16, 180, 294
Bst4CI ACNGT 2 cut(s) 36, 52
BstDEI CTNAG 2 cut(s) 72, 264
BstDSI CCRYGG 1 cut(s) 294
BstFNI CGCG 1 cut(s) 311
BstKTI GATC 2 cut(s) 111, 309
BstMBI GATC 2 cut(s) 108, 306
BstMWI GCNNNNNNNGC 3 cut(s) 146, 207, 219
BstSCI CCNGG 2 cut(s) 149, 230
BstSLI GKGCMC 1 cut(s) 50
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 2 cut(s) 197, 210
BstV2I GAAGAC 1 cut(s) 194
BstXI CCANNNNNNTGG 2 cut(s) 295, 301
BsuI GTATCC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 164, 179, 235
BtgI CCRYGG 1 cut(s) 294
Csp6I GTAC 2 cut(s) 37, 85
CviAII CATG 2 cut(s) 239, 295
CviJI RGCY 4 cut(s) 164, 179, 201, 235
CviKI_1 RGCY 4 cut(s) 164, 179, 201, 235
CviQI GTAC 2 cut(s) 37, 85
DdeI CTNAG 2 cut(s) 72, 264
DpnI GATC 2 cut(s) 110, 308
DpnII GATC 2 cut(s) 108, 306
DriI GACNNNNNGTC 1 cut(s) 38
Eam1105I GACNNNNNGTC 1 cut(s) 38
Eco130I CCWWGG 3 cut(s) 16, 180, 294
EcoT14I CCWWGG 3 cut(s) 16, 180, 294
ErhI CCWWGG 3 cut(s) 16, 180, 294
FaeI CATG 2 cut(s) 242, 298
FaiI YATR 3 cut(s) 147, 240, 296
FatI CATG 2 cut(s) 238, 294
Fnu4HI GCNGC 3 cut(s) 199, 211, 250
Fsp4HI GCNGC 3 cut(s) 199, 211, 250
GluI GCNGC 3 cut(s) 199, 211, 250
HaeIII GGCC 3 cut(s) 164, 179, 235
HapII CCGG 2 cut(s) 150, 232
Hin1II CATG 2 cut(s) 242, 298
HinfI GANTC 1 cut(s) 103
HpaII CCGG 2 cut(s) 150, 232
HphI GGTGA 2 cut(s) 16, 196
Hpy166II GTNNAC 2 cut(s) 121, 270
Hpy188I TCNGA 3 cut(s) 59, 102, 108
Hpy8I GTNNAC 2 cut(s) 121, 270
HpyCH4III ACNGT 2 cut(s) 36, 52
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 207, 219
HpyF3I CTNAG 2 cut(s) 72, 264
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 2 cut(s) 242, 298
Kzo9I GATC 2 cut(s) 108, 306
LpnPI CCDG 4 cut(s) 28, 95, 163, 245
Lsp1109I GCAGC 2 cut(s) 197, 210
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 242
MalI GATC 2 cut(s) 110, 308
MboI GATC 2 cut(s) 108, 306
MboII GAAGA 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 2 cut(s) 27, 277
MnlI CCTC 7 cut(s) 60, 65, 106, 106, 124, 127, 246
MspA1I CMGCKG 2 cut(s) 210, 249
MspI CCGG 2 cut(s) 150, 232
MspR9I CCNGG 2 cut(s) 151, 232
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 3 cut(s) 146, 207, 219
NciI CCSGG 2 cut(s) 151, 232
NcoI CCATGG 1 cut(s) 294
NdeII GATC 2 cut(s) 108, 306
NlaIII CATG 2 cut(s) 242, 298
NlaIV GGNNCC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 200, 212, 251
PspN4I GGNNCC 1 cut(s) 49
RsaI GTAC 2 cut(s) 38, 86
RsaNI GTAC 2 cut(s) 37, 85
SatI GCNGC 3 cut(s) 199, 211, 250
Sau3AI GATC 2 cut(s) 108, 306
ScrFI CCNGG 2 cut(s) 151, 232
SduI GDGCHC 1 cut(s) 50
SetI ASST 6 cut(s) 42, 71, 98, 138, 186, 228
Sse9I AATT 2 cut(s) 27, 277
SsiI CCGC 3 cut(s) 157, 208, 249
StyD4I CCNGG 2 cut(s) 149, 230
StyI CCWWGG 3 cut(s) 16, 180, 294
TaaI ACNGT 2 cut(s) 36, 52
TaiI ACGT 1 cut(s) 42
TaqI TCGA 2 cut(s) 77, 111
TasI AATT 2 cut(s) 27, 277
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 1 cut(s) 103
TseI GCWGC 2 cut(s) 198, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.