MD07G1212600.v1.1

Ribosomal protein L35Ae

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003771
Physical Location & Seq
Forward (+)
29055520 .. 29057351
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1212600.v1.1.491

Sequence Viewer

Length: 315 bp
ATGGTAAAGGGACGCCAAGGAGAGCGAATTAGACTCTACGTCAGGGCCACCGTTCTCGGATACAAGAGGTCTAAGTCGAACCAGTACCCGAACACCTCTCTGATTCAGATCGAGGGAGTGAACACCAAAGAGGAGGTGGCGTGGTATGCCGGGAAGCGGTTGGCCTACATTTACAAGGCCAAGGTGAAGACCAATGGCAGCCACTACCGCTGCATTTGGGGCAAGGTTTCCAGACCTCATGGTAACAGTGGCGTCGTTCGTGCTAAGTTCACTTCCAACTTGCCCCCCAAGTCCATGGTATTGGTGCTCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L35Ae

Protein Analysis

105

Amino Acids

11.8

Weight (kDa)

10.51

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35Ae PF01247 12 - 99 5.8e-41 Ribosomal protein L35Ae
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 157, 208
AcyI GRCGYC 2 cut(s) 13, 252
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 156
AhdI GACNNNNNGTC 1 cut(s) 38
Alw21I GWGCWC 1 cut(s) 309
AoxI GGCC 3 cut(s) 45, 162, 177
ApeKI GCWGC 2 cut(s) 198, 210
AspS9I GGNCC 1 cut(s) 45
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 196
BauI CACGAG 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 197, 210
BciVI GTATCC 1 cut(s) 53
BcnI CCSGG 1 cut(s) 151
BfuI GTATCC 1 cut(s) 53
BisI GCNGC 2 cut(s) 199, 211
BlsI GCNGC 2 cut(s) 200, 212
Bme1390I CCNGG 1 cut(s) 151
BmeRI GACNNNNNGTC 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 45
BmrFI CCNGG 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 107
BsaHI GRCGYC 2 cut(s) 13, 252
BsaJI CCNNGG 3 cut(s) 16, 180, 294
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 82
Bse8I GATNNNNATC 1 cut(s) 107
BseDI CCNNGG 3 cut(s) 16, 180, 294
BseJI GATNNNNATC 1 cut(s) 107
BseLI CCNNNNNNNGG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 2 cut(s) 197, 210
BshFI GGCC 3 cut(s) 47, 164, 179
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 3 cut(s) 47, 164, 179
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 1 cut(s) 108
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 2 cut(s) 157, 208
BspANI GGCC 3 cut(s) 47, 164, 179
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 3 cut(s) 16, 180, 294
BssMI GATC 1 cut(s) 108
BssNI GRCGYC 2 cut(s) 13, 252
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 3 cut(s) 16, 180, 294
Bst2BI CACGAG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 52, 248
BstACI GRCGYC 2 cut(s) 13, 252
BstDEI CTNAG 2 cut(s) 72, 264
BstDSI CCRYGG 1 cut(s) 294
BstKTI GATC 1 cut(s) 111
BstMBI GATC 1 cut(s) 108
BstMWI GCNNNNNNNGC 3 cut(s) 146, 207, 219
BstSCI CCNGG 1 cut(s) 149
BstV1I GCAGC 2 cut(s) 197, 210
BstV2I GAAGAC 1 cut(s) 194
BstXI CCANNNNNNTGG 2 cut(s) 295, 301
BsuI GTATCC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 47, 164, 179
BtgI CCRYGG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 45
CseI GACGC 2 cut(s) 21, 241
Csp6I GTAC 1 cut(s) 85
CviAII CATG 2 cut(s) 239, 295
CviJI RGCY 4 cut(s) 47, 164, 179, 201
CviKI_1 RGCY 4 cut(s) 47, 164, 179, 201
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 2 cut(s) 72, 264
DpnI GATC 1 cut(s) 110
DpnII GATC 1 cut(s) 108
DriI GACNNNNNGTC 1 cut(s) 38
Eam1105I GACNNNNNGTC 1 cut(s) 38
Eco130I CCWWGG 3 cut(s) 16, 180, 294
EcoT14I CCWWGG 3 cut(s) 16, 180, 294
ErhI CCWWGG 3 cut(s) 16, 180, 294
FaeI CATG 2 cut(s) 242, 298
FaiI YATR 3 cut(s) 147, 240, 296
FaqI GGGAC 1 cut(s) 24
FatI CATG 2 cut(s) 238, 294
Fnu4HI GCNGC 2 cut(s) 199, 211
Fsp4HI GCNGC 2 cut(s) 199, 211
GluI GCNGC 2 cut(s) 199, 211
HaeIII GGCC 3 cut(s) 47, 164, 179
HapII CCGG 1 cut(s) 150
HgaI GACGC 2 cut(s) 21, 241
Hin1I GRCGYC 2 cut(s) 13, 252
Hin1II CATG 2 cut(s) 242, 298
HinfI GANTC 2 cut(s) 33, 103
HpaII CCGG 1 cut(s) 150
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 121, 270
Hpy188I TCNGA 3 cut(s) 59, 102, 108
Hpy188III TCNNGA 1 cut(s) 231
Hpy8I GTNNAC 2 cut(s) 121, 270
Hpy99I CGWCG 1 cut(s) 257
HpyCH4III ACNGT 2 cut(s) 52, 248
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 207, 219
HpyF3I CTNAG 2 cut(s) 72, 264
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 2 cut(s) 13, 252
Hsp92II CATG 2 cut(s) 242, 298
Kzo9I GATC 1 cut(s) 108
LpnPI CCDG 4 cut(s) 28, 95, 163, 244
Lsp1109I GCAGC 2 cut(s) 197, 210
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 242
MalI GATC 1 cut(s) 110
MboI GATC 1 cut(s) 108
MboII GAAGA 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 1 cut(s) 27
MlyI GAGTC 1 cut(s) 27
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 6 cut(s) 60, 106, 106, 124, 127, 246
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 1 cut(s) 151
MwoI GCNNNNNNNGC 3 cut(s) 146, 207, 219
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 294
NdeII GATC 1 cut(s) 108
NlaIII CATG 2 cut(s) 242, 298
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 200, 212
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PspPI GGNCC 1 cut(s) 45
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SatI GCNGC 2 cut(s) 199, 211
Sau3AI GATC 1 cut(s) 108
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 1 cut(s) 27
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 309
SetI ASST 7 cut(s) 42, 71, 98, 138, 186, 228, 238
Sse9I AATT 1 cut(s) 27
SsiI CCGC 2 cut(s) 157, 208
StyD4I CCNGG 1 cut(s) 149
StyI CCWWGG 3 cut(s) 16, 180, 294
TaaI ACNGT 2 cut(s) 52, 248
TaiI ACGT 1 cut(s) 42
TaqI TCGA 2 cut(s) 77, 111
TasI AATT 1 cut(s) 27
TfiI GAWTC 1 cut(s) 103
TscAI CASTG 1 cut(s) 253
TseI GCWGC 2 cut(s) 198, 210
TspRI CASTG 1 cut(s) 253
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.