MD01G1145800.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007262
Physical Location & Seq
Forward (+)
25568336 .. 25569610
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1145800.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGCGGCGTGCTTCTTTCTCTCCCTTGCCGCCATCCTCTTCACCCTCTCTCTCCTCCCCGGAAGCACCTCGCTTTCCACCACCGTCGGTGCCACCTACTCCGCCAGCACCGCCGCAACCTACGCCGCCCCTCCCGCCGTCGACCGCGTTGCCCACGCCGTCTCCTCCCTCGGCCTCTCCGCGCTACGACTAGACGCCTCCGACCCCGCCATGGTCCGCGCCTTCCTCTACTCCAACACCTCCCTCCTCCTCACCATCCCCAACAAGCTCGTCCCGCCCCTCGCCGCAAACCGCTCCAACGCCCTCCGCTGGCTCTACATCCACGTCATCCCCTTCTACCCCCGCACCAAAATTTCCACCATCTCCGTCGGCAACGACCTCCTCGAGTCCTCTCCGAACTTCTCCACCTTCCTTCTTCCCGCCATCCGCAACGTTCAACTCTCCCTCAAAGACCTCGGCATCCAAAAGATCTCCGTCTCGACCACCTTCTCCTTCATCAACGTCATCACCACGGCGTTCCCGCCTTCCAATGCCCAGTTCCAAGAACCGGCCGTTGACACCGTCATCAGGCCCCTCCTCCAGTTCCTCCATGACACCAATTCCTCGTTCTTGATCAATCTCTACCCCTACAATCTGTACAAGATGCGCAGCGAGATACCGATCGGGTTTCCCCTGTTTCAGGAGCACCCATTCGGATTCCGAGACGACCTCACCACCGGCGTCCGGTACCGGAACCTCTTCGACATGATGGTCGACGCCGTCATCAGCGCCATGGCTGTCGCAGGATACGAGAACATTCCGGTGATCGTGACCGAGACTGGGTGGCCCAGCTTCAGCACCGACCCGGCCGAAATCGACGCCAACCCGGTTTACGCCGAGATGTACATCAAGGGGCTTTTGTGTCATTTGAGGTCCGGAAAGGGAACGCCGCTGAGGAGAGAAGGGGTTTCCGAGACGTACATTTACGAGCTGCTCGACAAGCAAGTGAAGCAGGGGCGCAATTGGGGGATTCTTTACCCAAATTTGACCAGCAAGTACAAGGAGATTCAGTATTCTGGGTCGGATTGCGGAGGTTTCTTTGATATCTTGATCATGGCGGTTGGGCAACTCTTGGTGTTTGCATTACTTGCCTGGTGA

Protein Analysis

379

Amino Acids

41.64

Weight (kDa)

6.52

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 50 - 347 9.4e-50 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 749
Acc16I TGCGCA 1 cut(s) 647
Acc65I GGTACC 1 cut(s) 726
AccB1I GGYRCC 2 cut(s) 89, 726
AccBSI CCGCTC 1 cut(s) 294
AccI GTMKAC 2 cut(s) 141, 753
AccII CGCG 3 cut(s) 147, 182, 219
AccIII TCCGGA 1 cut(s) 914
AclI AACGTT 1 cut(s) 432
AcoI YGGCCR 2 cut(s) 549, 846
AcsI RAATTY 2 cut(s) 351, 1021
AcuI CTGAAG 1 cut(s) 817
AcyI GRCGYC 4 cut(s) 195, 720, 756, 858
AfaI GTAC 5 cut(s) 638, 728, 884, 959, 1037
AfiI CCNNNNNNNGG 7 cut(s) 211, 309, 547, 567, 679, 723, 819
AgsI TTSAA 1 cut(s) 437
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 1130
AjuI GAANNNNNNNTTGG 4 cut(s) 521, 553, 590, 622
AluBI AGCT 3 cut(s) 268, 831, 970
AluI AGCT 3 cut(s) 268, 831, 970
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 5 cut(s) 166, 481, 696, 809, 947
Ama87I CYCGRG 1 cut(s) 383
Aor13HI TCCGGA 1 cut(s) 914
AoxI GGCC 5 cut(s) 172, 549, 569, 824, 846
ApeKI GCWGC 2 cut(s) 648, 970
ApoI RAATTY 2 cut(s) 351, 1021
Asp700I GAANNNNTTC 1 cut(s) 737
Asp718I GGTACC 1 cut(s) 726
AspLEI GCGC 5 cut(s) 184, 221, 648, 770, 999
AspS9I GGNCC 4 cut(s) 214, 570, 825, 912
AsuC2I CCSGG 3 cut(s) 60, 845, 866
AsuHPI GGTGA 5 cut(s) 34, 244, 499, 703, 814
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 2 cut(s) 214, 912
BanI GGYRCC 2 cut(s) 89, 726
Bbv12I GWGCWC 1 cut(s) 687
BbvCI CCTCAGC 1 cut(s) 932
BbvI GCAGC 2 cut(s) 660, 957
BccI CCATC 5 cut(s) 41, 263, 368, 431, 742
BceAI ACGGC 5 cut(s) 122, 143, 528, 536, 743
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 1 cut(s) 1132
BciVI GTATCC 1 cut(s) 779
BclI TGATCA 2 cut(s) 612, 1089
BcnI CCSGG 3 cut(s) 60, 845, 866
BcoDI GTCTC 5 cut(s) 166, 481, 696, 809, 947
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 771
BfuI GTATCC 1 cut(s) 779
BglII AGATCT 1 cut(s) 468
BisI GCNGC 8 cut(s) 6, 30, 114, 126, 285, 649, 929, 971
BlsI GCNGC 8 cut(s) 7, 31, 115, 127, 286, 650, 930, 972
Bme1390I CCNGG 4 cut(s) 60, 845, 866, 1132
Bme18I GGWCC 2 cut(s) 214, 912
BmeT110I CYCGRG 1 cut(s) 383
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 4 cut(s) 214, 570, 825, 912
BmiI GGNNCC 4 cut(s) 91, 572, 728, 734
BmrFI CCNGG 4 cut(s) 60, 845, 866, 1132
BmrI ACTGGG 2 cut(s) 529, 828
BmsI GCATC 2 cut(s) 468, 633
BmuI ACTGGG 2 cut(s) 529, 828
BplI GAGNNNNNCTC 2 cut(s) 693, 725
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 932
BpuMI CCSGG 3 cut(s) 60, 845, 866
BsaBI GATNNNNATC 2 cut(s) 659, 884
BsaHI GRCGYC 4 cut(s) 195, 720, 756, 858
BsaJI CCNNGG 6 cut(s) 58, 169, 210, 454, 510, 771
BsaWI WCCGGW 4 cut(s) 723, 729, 799, 914
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 1034, 1064
Bsc4I CCNNNNNNNGG 7 cut(s) 211, 309, 547, 567, 679, 723, 819
Bse118I RCCGGY 2 cut(s) 547, 716
Bse1I ACTGG 3 cut(s) 535, 580, 823
Bse8I GATNNNNATC 2 cut(s) 659, 884
BseAI TCCGGA 1 cut(s) 914
BseBI CCWGG 1 cut(s) 1132
BseDI CCNNGG 6 cut(s) 58, 169, 210, 454, 510, 771
BseGI GGATG 6 cut(s) 33, 255, 318, 327, 423, 459
BseJI GATNNNNATC 2 cut(s) 659, 884
BseLI CCNNNNNNNGG 7 cut(s) 211, 309, 547, 567, 679, 723, 819
BseMII CTCAG 1 cut(s) 923
BseNI ACTGG 3 cut(s) 535, 580, 823
BseRI GAGGAG 7 cut(s) 44, 154, 236, 239, 371, 566, 949
BseX3I CGGCCG 2 cut(s) 549, 846
BseXI GCAGC 2 cut(s) 660, 957
BseYI CCCAGC 1 cut(s) 827
Bsh1236I CGCG 3 cut(s) 147, 182, 219
Bsh1285I CGRYCG 4 cut(s) 145, 552, 663, 849
BshFI GGCC 5 cut(s) 174, 551, 571, 826, 848
BshNI GGYRCC 2 cut(s) 89, 726
BsiEI CGRYCG 4 cut(s) 145, 552, 663, 849
BsiHKAI GWGCWC 1 cut(s) 687
BsiHKCI CYCGRG 1 cut(s) 383
BsiSI CCGG 9 cut(s) 60, 548, 717, 724, 730, 800, 845, 866, 915
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 7 cut(s) 211, 309, 547, 567, 679, 723, 819
BsmAI GTCTC 5 cut(s) 166, 481, 696, 809, 947
BsmBI CGTCTC 4 cut(s) 166, 481, 696, 947
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 5 cut(s) 174, 551, 571, 826, 848
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 687
Bsp13I TCCGGA 1 cut(s) 914
Bsp1407I TGTACA 2 cut(s) 636, 882
Bsp143I GATC 5 cut(s) 468, 612, 660, 804, 1089
Bsp19I CCATGG 2 cut(s) 210, 771
BspANI GGCC 5 cut(s) 174, 551, 571, 826, 848
BspCNI CTCAG 1 cut(s) 924
BspEI TCCGGA 1 cut(s) 914
BspFNI CGCG 3 cut(s) 147, 182, 219
BspLI GGNNCC 4 cut(s) 91, 572, 728, 734
BspT107I GGYRCC 2 cut(s) 89, 726
BsrBI CCGCTC 1 cut(s) 294
BsrFI RCCGGY 2 cut(s) 547, 716
BsrGI TGTACA 2 cut(s) 636, 882
BsrI ACTGG 3 cut(s) 535, 580, 823
BssAI RCCGGY 2 cut(s) 547, 716
BssECI CCNNGG 6 cut(s) 58, 169, 210, 454, 510, 771
BssMI GATC 5 cut(s) 468, 612, 660, 804, 1089
BssNI GRCGYC 4 cut(s) 195, 720, 756, 858
BssT1I CCWWGG 2 cut(s) 210, 771
Bst2UI CCWGG 1 cut(s) 1132
Bst4CI ACNGT 2 cut(s) 85, 562
Bst6I CTCTTC 2 cut(s) 44, 743
BstACI GRCGYC 4 cut(s) 195, 720, 756, 858
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstAUI TGTACA 2 cut(s) 636, 882
BstC8I GCNNGC 3 cut(s) 10, 106, 311
BstDEI CTNAG 1 cut(s) 932
BstDSI CCRYGG 3 cut(s) 210, 510, 771
BstENI CCTNNNNNAGG 1 cut(s) 677
BstF5I GGATG 6 cut(s) 33, 255, 318, 327, 423, 459
BstFNI CGCG 3 cut(s) 147, 182, 219
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 5 cut(s) 184, 221, 648, 770, 999
BstKTI GATC 5 cut(s) 471, 615, 663, 807, 1092
BstMAI GTCTC 5 cut(s) 166, 481, 696, 809, 947
BstMBI GATC 5 cut(s) 468, 612, 660, 804, 1089
BstMCI CGRYCG 4 cut(s) 145, 552, 663, 849
BstMWI GCNNNNNNNGC 7 cut(s) 110, 122, 134, 274, 979, 988, 1127
BstNI CCWGG 1 cut(s) 1132
BstSCI CCNGG 4 cut(s) 58, 843, 864, 1130
BstUI CGCG 3 cut(s) 147, 182, 219
BstV1I GCAGC 2 cut(s) 660, 957
BstX2I RGATCY 1 cut(s) 468
BstYI RGATCY 1 cut(s) 468
BstZI CGGCCG 2 cut(s) 549, 846
BsuI GTATCC 1 cut(s) 779
BsuRI GGCC 5 cut(s) 174, 551, 571, 826, 848
BtgI CCRYGG 3 cut(s) 210, 510, 771
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 6 cut(s) 33, 255, 318, 327, 423, 459
Cac8I GCNNGC 3 cut(s) 10, 106, 311
CfoI GCGC 5 cut(s) 184, 221, 648, 770, 999
Cfr10I RCCGGY 2 cut(s) 547, 716
Cfr13I GGNCC 4 cut(s) 214, 570, 825, 912
CseI GACGC 4 cut(s) 203, 709, 764, 866
Csp6I GTAC 5 cut(s) 637, 727, 883, 958, 1036
CviAII CATG 5 cut(s) 211, 590, 745, 772, 1093
CviQI GTAC 5 cut(s) 637, 727, 883, 958, 1036
DdeI CTNAG 1 cut(s) 932
DpnI GATC 5 cut(s) 470, 614, 662, 806, 1091
DpnII GATC 5 cut(s) 468, 612, 660, 804, 1089
DrdI GACNNNNNNGTC 1 cut(s) 749
DseDI GACNNNNNNGTC 1 cut(s) 749
EaeI YGGCCR 2 cut(s) 549, 846
EagI CGGCCG 2 cut(s) 549, 846
Eam1104I CTCTTC 2 cut(s) 44, 743
EarI CTCTTC 2 cut(s) 44, 743
EciI GGCGGA 1 cut(s) 91
EclXI CGGCCG 2 cut(s) 549, 846
Eco130I CCWWGG 2 cut(s) 210, 771
Eco32I GATATC 1 cut(s) 1084
Eco47I GGWCC 2 cut(s) 214, 912
Eco52I CGGCCG 2 cut(s) 549, 846
Eco57I CTGAAG 1 cut(s) 817
Eco88I CYCGRG 1 cut(s) 383
EcoNI CCTNNNNNAGG 1 cut(s) 677
EcoO109I RGGNCCY 1 cut(s) 570
EcoRII CCWGG 1 cut(s) 1130
EcoRV GATATC 1 cut(s) 1084
EcoT14I CCWWGG 2 cut(s) 210, 771
ErhI CCWWGG 2 cut(s) 210, 771
Esp3I CGTCTC 4 cut(s) 166, 481, 696, 947
FaeI CATG 5 cut(s) 214, 593, 748, 775, 1096
FaiI YATR 5 cut(s) 212, 591, 746, 773, 1094
FaqI GGGAC 1 cut(s) 257
FatI CATG 5 cut(s) 210, 589, 744, 771, 1092
FauI CCCGC 6 cut(s) 142, 214, 282, 350, 427, 528
FbaI TGATCA 2 cut(s) 612, 1089
FblI GTMKAC 2 cut(s) 141, 753
Fnu4HI GCNGC 8 cut(s) 6, 30, 114, 126, 285, 649, 929, 971
FokI GGATG 6 cut(s) 20, 242, 305, 314, 410, 446
Fsp4HI GCNGC 8 cut(s) 6, 30, 114, 126, 285, 649, 929, 971
FspBI CTAG 1 cut(s) 191
FspI TGCGCA 1 cut(s) 647
GlaI GCGC 5 cut(s) 183, 220, 647, 769, 998
GluI GCNGC 8 cut(s) 6, 30, 114, 126, 285, 649, 929, 971
GsaI CCCAGC 1 cut(s) 831
GsuI CTGGAG 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 5 cut(s) 174, 551, 571, 826, 848
HapII CCGG 9 cut(s) 60, 548, 717, 724, 730, 800, 845, 866, 915
HgaI GACGC 4 cut(s) 203, 709, 764, 866
HhaI GCGC 5 cut(s) 184, 221, 648, 770, 999
Hin1I GRCGYC 4 cut(s) 195, 720, 756, 858
Hin1II CATG 5 cut(s) 214, 593, 748, 775, 1096
Hin6I GCGC 5 cut(s) 182, 219, 646, 768, 997
HinP1I GCGC 5 cut(s) 182, 219, 646, 768, 997
HincII GTYRAC 3 cut(s) 142, 556, 754
HindII GTYRAC 3 cut(s) 142, 556, 754
HinfI GANTC 4 cut(s) 386, 696, 1009, 1045
HpaII CCGG 9 cut(s) 60, 548, 717, 724, 730, 800, 845, 866, 915
HphI GGTGA 5 cut(s) 34, 244, 499, 703, 814
Hpy166II GTNNAC 4 cut(s) 142, 556, 754, 871
Hpy188I TCNGA 6 cut(s) 202, 396, 695, 701, 952, 1063
Hpy188III TCNNGA 6 cut(s) 478, 610, 680, 808, 915, 1087
Hpy8I GTNNAC 4 cut(s) 142, 556, 754, 871
Hpy99I CGWCG 5 cut(s) 89, 143, 371, 758, 860
HpyAV CCTTC 8 cut(s) 232, 343, 418, 422, 496, 502, 534, 935
HpyCH4III ACNGT 2 cut(s) 85, 562
HpyCH4IV ACGT 4 cut(s) 324, 432, 501, 956
HpyCH4V TGCA 1 cut(s) 1121
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 122, 134, 274, 979, 988, 1127
HpyF3I CTNAG 1 cut(s) 932
HpySE526I ACGT 4 cut(s) 324, 432, 501, 956
Hsp92I GRCGYC 4 cut(s) 195, 720, 756, 858
Hsp92II CATG 5 cut(s) 214, 593, 748, 775, 1096
HspAI GCGC 5 cut(s) 182, 219, 646, 768, 997
Kpn2I TCCGGA 1 cut(s) 914
KpnI GGTACC 1 cut(s) 730
Ksp22I TGATCA 2 cut(s) 612, 1089
Kzo9I GATC 5 cut(s) 468, 612, 660, 804, 1089
LmnI GCTCC 2 cut(s) 299, 682
Lsp1109I GCAGC 2 cut(s) 660, 957
LweI GCATC 2 cut(s) 468, 633
MaeI CTAG 1 cut(s) 191
MaeII ACGT 4 cut(s) 324, 432, 501, 956
MaeIII GTNAC 1 cut(s) 808
MalI GATC 5 cut(s) 470, 614, 662, 806, 1091
MbiI CCGCTC 1 cut(s) 294
MboI GATC 5 cut(s) 468, 612, 660, 804, 1089
MboII GAAGA 3 cut(s) 31, 407, 730
MfeI CAATTG 1 cut(s) 1000
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 4 cut(s) 351, 598, 1000, 1021
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 4 cut(s) 225, 258, 321, 1041
MroI TCCGGA 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 737
MslI CAYNNNNRTG 1 cut(s) 800
MspA1I CMGCKG 2 cut(s) 309, 931
MspI CCGG 9 cut(s) 60, 548, 717, 724, 730, 800, 845, 866, 915
MspR9I CCNGG 4 cut(s) 60, 845, 866, 1132
MunI CAATTG 1 cut(s) 1000
MvaI CCWGG 1 cut(s) 1132
MvnI CGCG 3 cut(s) 147, 182, 219
MwoI GCNNNNNNNGC 7 cut(s) 110, 122, 134, 274, 979, 988, 1127
NciI CCSGG 3 cut(s) 60, 845, 866
NcoI CCATGG 2 cut(s) 210, 771
NdeII GATC 5 cut(s) 468, 612, 660, 804, 1089
NlaIII CATG 5 cut(s) 214, 593, 748, 775, 1096
NlaIV GGNNCC 4 cut(s) 91, 572, 728, 734
NmeAIII GCCGAG 3 cut(s) 150, 435, 901
NmuCI GTSAC 1 cut(s) 808
NsbI TGCGCA 1 cut(s) 647
PaeR7I CTCGAG 1 cut(s) 383
PcsI WCGNNNNNNNCGW 3 cut(s) 381, 786, 972
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 3 cut(s) 696, 1009, 1045
PflFI GACNNNGTC 2 cut(s) 560, 758
PkrI GCNGC 8 cut(s) 7, 31, 115, 127, 286, 650, 930, 972
Ple19I CGATCG 1 cut(s) 663
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Psp1406I AACGTT 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 1130
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 1 cut(s) 1130
PspN4I GGNNCC 4 cut(s) 91, 572, 728, 734
PspPI GGNCC 4 cut(s) 214, 570, 825, 912
PspXI VCTCGAGB 1 cut(s) 383
PsuI RGATCY 1 cut(s) 468
PsyI GACNNNGTC 2 cut(s) 560, 758
PvuI CGATCG 1 cut(s) 663
RsaI GTAC 5 cut(s) 638, 728, 884, 959, 1037
RsaNI GTAC 5 cut(s) 637, 727, 883, 958, 1036
RseI CAYNNNNRTG 1 cut(s) 800
SalI GTCGAC 2 cut(s) 140, 752
SatI GCNGC 8 cut(s) 6, 30, 114, 126, 285, 649, 929, 971
Sau3AI GATC 5 cut(s) 468, 612, 660, 804, 1089
Sau96I GGNCC 4 cut(s) 214, 570, 825, 912
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 4 cut(s) 60, 845, 866, 1132
SduI GDGCHC 1 cut(s) 687
SfaNI GCATC 2 cut(s) 468, 633
Sfr274I CTCGAG 1 cut(s) 383
SgrAI CRCCGGYG 1 cut(s) 716
SinI GGWCC 2 cut(s) 214, 912
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 1 cut(s) 800
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 4 cut(s) 351, 598, 1000, 1021
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 58, 843, 864, 1130
StyI CCWWGG 2 cut(s) 210, 771
TaaI ACNGT 2 cut(s) 85, 562
TaiI ACGT 4 cut(s) 327, 435, 504, 959
TaqI TCGA 7 cut(s) 141, 384, 479, 741, 753, 855, 975
TaqII GACCGA 1 cut(s) 827
TasI AATT 4 cut(s) 351, 598, 1000, 1021
TatI WGTACW 3 cut(s) 636, 882, 1035
TauI GCSGC 6 cut(s) 8, 32, 116, 128, 287, 931
TfiI GAWTC 3 cut(s) 696, 1009, 1045
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 2 cut(s) 648, 970
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 1 cut(s) 484
TspGWI ACGGA 2 cut(s) 355, 463
Tth111I GACNNNGTC 2 cut(s) 560, 758
VpaK11BI GGWCC 2 cut(s) 214, 912
XagI CCTNNNNNAGG 1 cut(s) 677
XapI RAATTY 2 cut(s) 351, 1021
XhoI CTCGAG 1 cut(s) 383
XmiI GTMKAC 2 cut(s) 141, 753
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.