Rroxscaffold_4G00287820

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007262
Physical Location & Seq
Reverse (-)
8774312 .. 8775490
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00287820.1

Sequence Viewer

Length: 1179 bp
ATGGCTTCCCACTTCTCTCTCTCCGCCATCTCTCTCCTCACCCTCCTCTCTCTCCTCCTCACAGCAACCGCTTCGCCTGACACAGCCTCCACGGTCATCGGCGCCACCTACTCAGCCTCCACCGCCGCAACCTACCCCACCCCTCCCACCCCGGAGCGCGTGGCCTCCGCAGTCTCCTCCCTCGGCCTCTCCTCGCTCCGCCTCGACGTCTCCGACCCCGGCCTCGTCCGCGCCTTCCTCTACTCCAACACCACGCTCCTCCTCACCATCCCCAACGCGCTGGTCCCGCCACTCGCCGCCAACCGCTCCAACGCCATGCGGTGGCTCTACGCGCACGTCGTCCCCTTCTTCCCGCGCACCAAGATCGCCGCCATCTCCGTCGGCAACGACGTCCTAGAGTCCACGCCGGAGTTCTCGCAGTTCCTCCTCCCGGCGATCCAGAACGTCCACCTGGCGCTCCTCGACCTCGGGATCCGCAAGATCGCCGTCTCCACCACCTTCTCCTTCGTCAACGTCGTCACGACGCCGTTTCCTCCCTCCGCCGGGCAGTTCCAGGACGCGCCCCTCGAGACGGTGATCAGGCCTCTGCTTCAGTTCTTGCGCGACACCAACTCCTCCTTCTTCATCAACGTCTACCCCTACAACCTCTACCGGCTCCGGAGTGAGATTCCGATTGGGTTCCCTCTCTTTCAGGAGCACCCGTTCAGTTTCCGAGACGACATGACCACCGGCGTGCGCTACCGGAATCTCTTCGACATGATGGTCGACGCCGTCATCTCAGCCATGGCCGTCGCCGGACACGAGAACATTCCGCTGATCGTGACTGAAACGGGTTGGCCCAGCTCCAGCACCGACCCGAGAGAAGTAGACGCCAACCCGGTTTACGCGGAGCTCTACATCAAGGGGCTATTGGGCCACCTCAGGTCGGGTCGGGGAACGCCGCTCCGAAAAGAAGGGGTGCCTCTGGTTTACATCTATGAGCTGATTGATAAGCAAGTGAGGCAGGGACGCAACTGGGGAATTCTTTTTCCGAATATGACCAAGAAGTACAAGAGCATTGACTACAGCTCTGGCTCTGATAGCGTTCTTGGGGGTTTCGGATTCTTGAAGATGGTGGTTGGTCAATTCTTGGTCTTTGCTTTCCTCTTGCGTGGTAATGCTGCTATTTCTCTGCTCTAG

Protein Analysis

392

Amino Acids

42.9

Weight (kDa)

8.67

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 49 - 351 1.7e-48 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 761
AatII GACGTC 2 cut(s) 210, 393
AccB1I GGYRCC 2 cut(s) 101, 958
AccB7I CCANNNNNTGG 1 cut(s) 321
AccBSI CCGCTC 2 cut(s) 306, 943
AccI GTMKAC 3 cut(s) 633, 765, 867
AccII CGCG 8 cut(s) 159, 231, 278, 332, 355, 560, 603, 887
AccIII TCCGGA 1 cut(s) 657
AclWI GGATC 3 cut(s) 430, 466, 479
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 1 cut(s) 1020
AcuI CTGAAG 1 cut(s) 575
AcyI GRCGYC 6 cut(s) 102, 207, 390, 524, 768, 870
AfaI GTAC 1 cut(s) 1049
AfiI CCNNNNNNNGG 6 cut(s) 321, 430, 542, 543, 571, 925
AgsI TTSAA 1 cut(s) 1108
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 450, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AleI CACNNNNGTG 1 cut(s) 731
AloI GAACNNNNNNTCC 2 cut(s) 686, 718
AluBI AGCT 4 cut(s) 843, 892, 982, 1068
AluI AGCT 4 cut(s) 843, 892, 982, 1068
Alw21I GWGCWC 2 cut(s) 699, 894
Alw26I GTCTC 5 cut(s) 178, 214, 493, 563, 708
AlwI GGATC 3 cut(s) 430, 466, 479
Ama87I CYCGRG 3 cut(s) 467, 566, 856
Aor13HI TCCGGA 1 cut(s) 657
AoxI GGCC 7 cut(s) 162, 184, 220, 581, 786, 836, 913
ApeKI GCWGC 1 cut(s) 1160
ApoI RAATTY 1 cut(s) 1020
Asp700I GAANNNNTTC 1 cut(s) 749
AspS9I GGNCC 3 cut(s) 283, 837, 913
AsuC2I CCSGG 5 cut(s) 152, 219, 431, 544, 878
AsuHPI GGTGA 3 cut(s) 31, 256, 586
AvaI CYCGRG 3 cut(s) 467, 566, 856
AvaII GGWCC 1 cut(s) 283
AxyI CCTNAGG 1 cut(s) 920
BamHI GGATCC 1 cut(s) 471
BanI GGYRCC 2 cut(s) 101, 958
BanII GRGCYC 1 cut(s) 894
BauI CACGAG 1 cut(s) 800
Bbv12I GWGCWC 2 cut(s) 699, 894
BbvI GCAGC 1 cut(s) 1147
BccI CCATC 5 cut(s) 35, 275, 380, 754, 1105
BceAI ACGGC 4 cut(s) 470, 511, 755, 773
BcgI CGANNNNNNTGC 4 cut(s) 54, 88, 346, 380
BciT130I CCWGG 2 cut(s) 452, 554
BclI TGATCA 1 cut(s) 576
BcnI CCSGG 5 cut(s) 152, 219, 431, 544, 878
BcoDI GTCTC 5 cut(s) 178, 214, 493, 563, 708
BfaI CTAG 2 cut(s) 395, 1177
BfmI CTRYAG 1 cut(s) 1063
BfoI RGCGCY 2 cut(s) 105, 458
BisI GCNGC 5 cut(s) 126, 297, 369, 941, 1161
BlsI GCNGC 5 cut(s) 127, 298, 370, 942, 1162
Bme1390I CCNGG 7 cut(s) 152, 219, 431, 452, 544, 554, 878
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 3 cut(s) 467, 566, 856
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 3 cut(s) 283, 837, 913
BmiI GGNNCC 6 cut(s) 103, 285, 473, 656, 680, 960
BmrFI CCNGG 7 cut(s) 152, 219, 431, 452, 544, 554, 878
BmrI ACTGGG 1 cut(s) 1024
BmuI ACTGGG 1 cut(s) 1024
BpmI CTGGAG 1 cut(s) 829
BpuMI CCSGG 5 cut(s) 152, 219, 431, 544, 878
BsaHI GRCGYC 6 cut(s) 102, 207, 390, 524, 768, 870
BsaJI CCNNGG 6 cut(s) 90, 150, 181, 217, 466, 783
BsaWI WCCGGW 2 cut(s) 657, 741
BsaXI ACNNNNNCTCC 8 cut(s) 71, 101, 101, 131, 596, 626, 686, 716
Bsc4I CCNNNNNNNGG 6 cut(s) 321, 430, 542, 543, 571, 925
Bse118I RCCGGY 2 cut(s) 651, 728
Bse1I ACTGG 1 cut(s) 1019
Bse21I CCTNAGG 1 cut(s) 920
BseAI TCCGGA 1 cut(s) 657
BseBI CCWGG 2 cut(s) 452, 554
BseDI CCNNGG 6 cut(s) 90, 150, 181, 217, 466, 783
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 6 cut(s) 321, 430, 542, 543, 571, 925
BseMII CTCAG 3 cut(s) 126, 792, 934
BseNI ACTGG 1 cut(s) 1019
BseXI GCAGC 1 cut(s) 1147
BseYI CCCAGC 1 cut(s) 839
Bsh1236I CGCG 8 cut(s) 159, 231, 278, 332, 355, 560, 603, 887
BshFI GGCC 7 cut(s) 164, 186, 222, 583, 788, 838, 915
BshNI GGYRCC 2 cut(s) 101, 958
BsiHKAI GWGCWC 2 cut(s) 699, 894
BsiHKCI CYCGRG 3 cut(s) 467, 566, 856
BslFI GGGAC 3 cut(s) 269, 326, 1020
BslI CCNNNNNNNGG 6 cut(s) 321, 430, 542, 543, 571, 925
BsmAI GTCTC 5 cut(s) 178, 214, 493, 563, 708
BsmBI CGTCTC 4 cut(s) 214, 493, 563, 708
BsmFI GGGAC 3 cut(s) 269, 326, 1020
BsnI GGCC 7 cut(s) 164, 186, 222, 583, 788, 838, 915
BsoBI CYCGRG 3 cut(s) 467, 566, 856
Bsp1286I GDGCHC 2 cut(s) 699, 894
Bsp13I TCCGGA 1 cut(s) 657
Bsp143I GATC 6 cut(s) 363, 435, 471, 480, 576, 816
Bsp19I CCATGG 1 cut(s) 783
BspANI GGCC 7 cut(s) 164, 186, 222, 583, 788, 838, 915
BspCNI CTCAG 3 cut(s) 125, 791, 933
BspEI TCCGGA 1 cut(s) 657
BspFNI CGCG 8 cut(s) 159, 231, 278, 332, 355, 560, 603, 887
BspLI GGNNCC 6 cut(s) 103, 285, 473, 656, 680, 960
BspPI GGATC 3 cut(s) 430, 466, 479
BspT107I GGYRCC 2 cut(s) 101, 958
BsrBI CCGCTC 2 cut(s) 306, 943
BsrFI RCCGGY 2 cut(s) 651, 728
BsrI ACTGG 1 cut(s) 1019
BssAI RCCGGY 2 cut(s) 651, 728
BssECI CCNNGG 6 cut(s) 90, 150, 181, 217, 466, 783
BssMI GATC 6 cut(s) 363, 435, 471, 480, 576, 816
BssNI GRCGYC 6 cut(s) 102, 207, 390, 524, 768, 870
BssSI CACGAG 1 cut(s) 800
BssT1I CCWWGG 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 800
Bst2UI CCWGG 2 cut(s) 452, 554
Bst4CI ACNGT 2 cut(s) 94, 574
Bst6I CTCTTC 1 cut(s) 755
BstACI GRCGYC 6 cut(s) 102, 207, 390, 524, 768, 870
BstC8I GCNNGC 1 cut(s) 734
BstDEI CTNAG 3 cut(s) 112, 778, 920
BstDSI CCRYGG 2 cut(s) 90, 783
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 8 cut(s) 159, 231, 278, 332, 355, 560, 603, 887
BstH2I RGCGCY 2 cut(s) 105, 458
BstKTI GATC 6 cut(s) 366, 438, 474, 483, 579, 819
BstMAI GTCTC 5 cut(s) 178, 214, 493, 563, 708
BstMBI GATC 6 cut(s) 363, 435, 471, 480, 576, 816
BstMWI GCNNNNNNNGC 6 cut(s) 122, 228, 286, 331, 1000, 1080
BstNI CCWGG 2 cut(s) 452, 554
BstSCI CCNGG 7 cut(s) 150, 217, 429, 450, 542, 552, 876
BstSFI CTRYAG 1 cut(s) 1063
BstUI CGCG 8 cut(s) 159, 231, 278, 332, 355, 560, 603, 887
BstV1I GCAGC 1 cut(s) 1147
BstX2I RGATCY 1 cut(s) 471
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 1 cut(s) 471
Bsu36I CCTNAGG 1 cut(s) 920
BsuRI GGCC 7 cut(s) 164, 186, 222, 583, 788, 838, 915
BtgI CCRYGG 2 cut(s) 90, 783
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 1 cut(s) 267
Cac8I GCNNGC 1 cut(s) 734
Cfr10I RCCGGY 2 cut(s) 651, 728
Cfr13I GGNCC 3 cut(s) 283, 837, 913
CseI GACGC 5 cut(s) 532, 566, 776, 878, 1017
Csp6I GTAC 1 cut(s) 1048
CviAII CATG 4 cut(s) 316, 721, 757, 784
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 3 cut(s) 112, 778, 920
DinI GGCGCC 1 cut(s) 103
DpnI GATC 6 cut(s) 365, 437, 473, 482, 578, 818
DpnII GATC 6 cut(s) 363, 435, 471, 480, 576, 816
DrdI GACNNNNNNGTC 1 cut(s) 761
DseDI GACNNNNNNGTC 1 cut(s) 761
EaeI YGGCCR 1 cut(s) 786
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
EciI GGCGGA 3 cut(s) 13, 188, 529
Ecl136II GAGCTC 1 cut(s) 892
Eco130I CCWWGG 1 cut(s) 783
Eco147I AGGCCT 1 cut(s) 583
Eco24I GRGCYC 1 cut(s) 894
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 892
Eco57I CTGAAG 1 cut(s) 575
Eco81I CCTNAGG 1 cut(s) 920
Eco88I CYCGRG 3 cut(s) 467, 566, 856
EcoICRI GAGCTC 1 cut(s) 892
EcoRI GAATTC 1 cut(s) 1020
EcoRII CCWGG 2 cut(s) 450, 552
EcoT14I CCWWGG 1 cut(s) 783
EcoT38I GRGCYC 1 cut(s) 894
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 783
Esp3I CGTCTC 4 cut(s) 214, 493, 563, 708
FaeI CATG 4 cut(s) 319, 724, 760, 787
FaiI YATR 6 cut(s) 317, 722, 758, 785, 978, 1037
FaqI GGGAC 3 cut(s) 269, 326, 1020
FatI CATG 4 cut(s) 315, 720, 756, 783
FauI CCCGC 2 cut(s) 294, 360
FbaI TGATCA 1 cut(s) 576
FblI GTMKAC 3 cut(s) 633, 765, 867
Fnu4HI GCNGC 5 cut(s) 126, 297, 369, 941, 1161
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 5 cut(s) 126, 297, 369, 941, 1161
FspBI CTAG 2 cut(s) 395, 1177
GluI GCNGC 5 cut(s) 126, 297, 369, 941, 1161
GsaI CCCAGC 1 cut(s) 843
GsuI CTGGAG 1 cut(s) 829
HaeII RGCGCY 2 cut(s) 105, 458
HaeIII GGCC 7 cut(s) 164, 186, 222, 583, 788, 838, 915
HgaI GACGC 5 cut(s) 532, 566, 776, 878, 1017
Hin1I GRCGYC 6 cut(s) 102, 207, 390, 524, 768, 870
Hin1II CATG 4 cut(s) 319, 724, 760, 787
HincII GTYRAC 2 cut(s) 511, 766
HindII GTYRAC 2 cut(s) 511, 766
HinfI GANTC 4 cut(s) 398, 667, 745, 1101
HphI GGTGA 3 cut(s) 31, 256, 586
Hpy166II GTNNAC 8 cut(s) 402, 448, 511, 634, 766, 868, 883, 970
Hpy188I TCNGA 7 cut(s) 214, 672, 713, 947, 1032, 1078, 1100
Hpy188III TCNNGA 8 cut(s) 439, 469, 520, 568, 658, 692, 820, 1105
Hpy8I GTNNAC 8 cut(s) 402, 448, 511, 634, 766, 868, 883, 970
Hpy99I CGWCG 8 cut(s) 209, 341, 383, 392, 518, 526, 770, 794
HpyAV CCTTC 6 cut(s) 244, 355, 508, 514, 628, 947
HpyCH4III ACNGT 2 cut(s) 94, 574
HpyCH4IV ACGT 6 cut(s) 207, 336, 390, 444, 513, 630
HpyF10VI GCNNNNNNNGC 6 cut(s) 122, 228, 286, 331, 1000, 1080
HpyF3I CTNAG 3 cut(s) 112, 778, 920
HpySE526I ACGT 6 cut(s) 207, 336, 390, 444, 513, 630
Hsp92I GRCGYC 6 cut(s) 102, 207, 390, 524, 768, 870
Hsp92II CATG 4 cut(s) 319, 724, 760, 787
KasI GGCGCC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 657
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 6 cut(s) 363, 435, 471, 480, 576, 816
Lsp1109I GCAGC 1 cut(s) 1147
MaeI CTAG 2 cut(s) 395, 1177
MaeII ACGT 6 cut(s) 207, 336, 390, 444, 513, 630
MaeIII GTNAC 2 cut(s) 517, 820
MalI GATC 6 cut(s) 365, 437, 473, 482, 578, 818
MbiI CCGCTC 2 cut(s) 306, 943
MboI GATC 6 cut(s) 363, 435, 471, 480, 576, 816
MboII GAAGA 4 cut(s) 340, 613, 742, 1120
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 2 cut(s) 699, 894
MluCI AATT 2 cut(s) 1020, 1124
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 3 cut(s) 237, 270, 333
MroI TCCGGA 1 cut(s) 657
MroXI GAANNNNTTC 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 814
MspR9I CCNGG 7 cut(s) 152, 219, 431, 452, 544, 554, 878
MvaI CCWGG 2 cut(s) 452, 554
MvnI CGCG 8 cut(s) 159, 231, 278, 332, 355, 560, 603, 887
MwoI GCNNNNNNNGC 6 cut(s) 122, 228, 286, 331, 1000, 1080
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 5 cut(s) 152, 219, 431, 544, 878
NcoI CCATGG 1 cut(s) 783
NdeII GATC 6 cut(s) 363, 435, 471, 480, 576, 816
NlaIII CATG 4 cut(s) 319, 724, 760, 787
NlaIV GGNNCC 6 cut(s) 103, 285, 473, 656, 680, 960
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 2 cut(s) 517, 820
OliI CACNNNNGTG 1 cut(s) 731
PaeR7I CTCGAG 1 cut(s) 566
PceI AGGCCT 1 cut(s) 583
PcsI WCGNNNNNNNCGW 6 cut(s) 210, 336, 387, 513, 564, 798
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 3 cut(s) 667, 745, 1101
PflFI GACNNNGTC 1 cut(s) 770
PflMI CCANNNNNTGG 1 cut(s) 321
PfoI TCCNGGA 1 cut(s) 552
PkrI GCNGC 5 cut(s) 127, 298, 370, 942, 1162
PleI GAGTC 1 cut(s) 406
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 406
Psp124BI GAGCTC 1 cut(s) 894
Psp6I CCWGG 2 cut(s) 450, 552
PspFI CCCAGC 1 cut(s) 839
PspGI CCWGG 2 cut(s) 450, 552
PspN4I GGNNCC 6 cut(s) 103, 285, 473, 656, 680, 960
PspPI GGNCC 3 cut(s) 283, 837, 913
PsuI RGATCY 1 cut(s) 471
PsyI GACNNNGTC 1 cut(s) 770
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 1 cut(s) 731
SacI GAGCTC 1 cut(s) 894
SalI GTCGAC 1 cut(s) 764
SatI GCNGC 5 cut(s) 126, 297, 369, 941, 1161
Sau3AI GATC 6 cut(s) 363, 435, 471, 480, 576, 816
Sau96I GGNCC 3 cut(s) 283, 837, 913
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 7 cut(s) 152, 219, 431, 452, 544, 554, 878
SduI GDGCHC 2 cut(s) 699, 894
SfcI CTRYAG 1 cut(s) 1063
SfoI GGCGCC 1 cut(s) 103
Sfr274I CTCGAG 1 cut(s) 566
SgrAI CRCCGGYG 1 cut(s) 728
SinI GGWCC 1 cut(s) 283
SlaI CTCGAG 1 cut(s) 566
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 2 cut(s) 1020, 1124
SseBI AGGCCT 1 cut(s) 583
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 395, 1177
SstI GAGCTC 1 cut(s) 894
StuI AGGCCT 1 cut(s) 583
StyD4I CCNGG 7 cut(s) 150, 217, 429, 450, 542, 552, 876
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 2 cut(s) 94, 574
TaiI ACGT 6 cut(s) 210, 339, 393, 447, 516, 633
TaqI TCGA 5 cut(s) 204, 462, 567, 753, 765
TasI AATT 2 cut(s) 1020, 1124
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 4 cut(s) 128, 299, 371, 943
TfiI GAWTC 3 cut(s) 667, 745, 1101
TseFI GTSAC 2 cut(s) 517, 820
TseI GCWGC 1 cut(s) 1160
Tsp45I GTSAC 2 cut(s) 517, 820
TspDTI ATGAA 1 cut(s) 613
TspGWI ACGGA 1 cut(s) 367
Tth111I GACNNNGTC 1 cut(s) 770
Van91I CCANNNNNTGG 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 1 cut(s) 1020
XhoI CTCGAG 1 cut(s) 566
XmiI GTMKAC 3 cut(s) 633, 765, 867
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 2 cut(s) 395, 1177
ZraI GACGTC 2 cut(s) 208, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.