Rorug01G0349300

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007262
Physical Location & Seq
Reverse (-)
46687934 .. 46691282
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0349300.1

Sequence Viewer

Length: 1254 bp
ATGCGGCGCGCCATAGACGCCCACCTCAAGACCATGCGTCTCTTTCCTCCCCTCTCTTCCTTCTCCTCTTCTTCTCCTTCTTCTTCTTCTTCCTCGCCTCTCTTCTCCGTCCGTACATTTCTGACCCTAGCCCAGCCGCGCTCCGTTTCCAATTCCGTCTCCAAAACCCTAATTTTCCACTCAATCGCCACTCCTTCCTACACTTCTTCTTCTTCCTCCTCATCGATTCCCAGCCTCAGAAGCCACAGACTTGGGCCGGCTATTGGGCTCTTTGCCGTGCGGTGCGTTTCGACGGCCTCGCCGGAGAGCAGTTTGGACTGGAACGAGCCGGTTTCGTGCTCCGAGGTCGGCGACGGCAGCAAAGGAAGTGTTGATAAGGATGATAAGCCCAATATTCCGGTCAGAGCTTACTTCTTTTCCACTAGCGTGGATTTGAGAAGCTTGGTGGAGCAGAACAGGCTGAATTTCATCCCTCCTACGTCGCGAATGACCAATTATGTTGTGTTTAAATTCGGGGAGCTTACCAAGTCTGACAATGCTTTGGGTCCTAGTTTAAGTGGGAGCGACTGTTGCTACATGGTAGTTTTTCAATATGGGTCCATTGTCTTGTTTAATGTTCGTGAACATGAGATTGATGGGTACTTGAAAATTGTGGAAAAACATGCATCTGGATTGCTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAGAAACCAGATTTAGACACATGGATGGAAGGCGGATTGGATTACATAATGTTGCAGCACTTGAATACTGATGGTATTCGTACCATTGGTAGTGTTCTTGGTCAAAGTATTGCTCTGGATTACTACGTCCGCCAGGTTGATGGAATGGTTGCAGAGTTTACTGACATTAATCGTGAAATGGAAACTACTGGAAAATTTAAGATGAAGAGGAAGAAACTATTTCAACTGGTGGGGAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTTGGACTTTTTGAGAGATCAGACATTGCCTGGAAGGATGCCAAATATGCTCAGATATGGGAATACCTCAGGGATGAATTTGAATTAACTCAGAGATTTGCTAGTCTTGACTTTAAGTTGAAGTTTGTGGAGCACAATATTCGCTTTCTTCAGGAAATTCTTCAAAACCGGAAATCAGATTTTTTGGAGTGGCTCATCATTGCACTAATAGGTGCTGAGATTATGCTCTCTCTTTATGACATTCTCCACAGATCAGCACTTGCTTCACTTTAG

Protein Analysis

417

Amino Acids

46.99

Weight (kDa)

6.73

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 193 - 363 9.7e-35 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 9, 139, 484
AciI CCGC 5 cut(s) 4, 137, 280, 744, 841
AcsI RAATTY 6 cut(s) 463, 509, 905, 952, 1058, 1137
AcuI CTGAAG 1 cut(s) 1115
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 115, 641, 793
AfiI CCNNNNNNNGG 1 cut(s) 263
AflII CTTAAG 1 cut(s) 977
AgsI TTSAA 7 cut(s) 590, 646, 775, 935, 1064, 1102, 1145
AhdI GACNNNNNGTC 1 cut(s) 36
AjnI CCWGG 2 cut(s) 843, 1010
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 4 cut(s) 407, 441, 520, 982
AluI AGCT 4 cut(s) 407, 441, 520, 982
Alw21I GWGCWC 2 cut(s) 341, 1116
Alw26I GTCTC 2 cut(s) 44, 163
AoxI GGCC 2 cut(s) 254, 294
ApeKI GCWGC 2 cut(s) 357, 766
ApoI RAATTY 6 cut(s) 463, 509, 905, 952, 1058, 1137
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 1 cut(s) 1140
AspLEI GCGC 3 cut(s) 9, 11, 141
AspS9I GGNCC 3 cut(s) 254, 545, 597
AsuHPI GGTGA 1 cut(s) 718
AvaII GGWCC 2 cut(s) 545, 597
AxyI CCTNAGG 1 cut(s) 1049
BanII GRGCYC 1 cut(s) 270
Bbv12I GWGCWC 2 cut(s) 341, 1116
BbvI GCAGC 2 cut(s) 369, 778
BccI CCATC 4 cut(s) 629, 730, 776, 845
BceAI ACGGC 3 cut(s) 260, 309, 370
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciT130I CCWGG 2 cut(s) 845, 1012
BcoDI GTCTC 2 cut(s) 44, 163
BfaI CTAG 4 cut(s) 128, 423, 549, 1083
BfrI CTTAAG 1 cut(s) 977
BisI GCNGC 4 cut(s) 5, 137, 358, 767
BlsI GCNGC 4 cut(s) 6, 138, 359, 768
Bme1390I CCNGG 2 cut(s) 845, 1012
Bme18I GGWCC 2 cut(s) 545, 597
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 254, 545, 597
BmiI GGNNCC 2 cut(s) 546, 598
BmrFI CCNGG 2 cut(s) 845, 1012
BmsI GCATC 2 cut(s) 674, 1009
BpuEI CTTGAG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 342
BsaWI WCCGGW 2 cut(s) 397, 1149
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse118I RCCGGY 2 cut(s) 256, 328
Bse1I ACTGG 3 cut(s) 323, 904, 942
Bse21I CCTNAGG 1 cut(s) 1049
Bse3DI GCAATG 2 cut(s) 1005, 1179
BseBI CCWGG 2 cut(s) 845, 1012
BseCI ATCGAT 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 6 cut(s) 385, 468, 700, 741, 1024, 1060
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 2 cut(s) 1005, 1179
BseMII CTCAG 5 cut(s) 250, 1046, 1063, 1085, 1188
BseNI ACTGG 3 cut(s) 323, 904, 942
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 2 cut(s) 55, 208
BseXI GCAGC 2 cut(s) 369, 778
BseYI CCCAGC 2 cut(s) 132, 230
Bsh1236I CGCG 3 cut(s) 9, 139, 484
BshFI GGCC 2 cut(s) 256, 296
BshVI ATCGAT 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 341, 1116
BsiSI CCGG 5 cut(s) 257, 302, 329, 398, 1150
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 2 cut(s) 44, 163
BsmBI CGTCTC 2 cut(s) 44, 163
BsnI GGCC 2 cut(s) 256, 296
Bsp1286I GDGCHC 3 cut(s) 270, 341, 1116
Bsp143I GATC 2 cut(s) 998, 1232
Bsp68I TCGCGA 1 cut(s) 484
BspACI CCGC 5 cut(s) 4, 137, 280, 744, 841
BspANI GGCC 2 cut(s) 256, 296
BspCNI CTCAG 5 cut(s) 249, 1045, 1062, 1084, 1189
BspDI ATCGAT 1 cut(s) 224
BspFNI CGCG 3 cut(s) 9, 139, 484
BspLI GGNNCC 2 cut(s) 546, 598
BspTI CTTAAG 1 cut(s) 977
BsrDI GCAATG 2 cut(s) 1005, 1179
BsrFI RCCGGY 2 cut(s) 256, 328
BsrI ACTGG 3 cut(s) 323, 904, 942
BssAI RCCGGY 2 cut(s) 256, 328
BssECI CCNNGG 1 cut(s) 342
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 2 cut(s) 998, 1232
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 845, 1012
Bst4CI ACNGT 1 cut(s) 569
Bst6I CTCTTC 4 cut(s) 61, 73, 107, 911
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 977
BstC8I GCNNGC 2 cut(s) 9, 258
BstDEI CTNAG 5 cut(s) 236, 1032, 1049, 1071, 1197
BstF5I GGATG 6 cut(s) 385, 468, 700, 741, 1024, 1060
BstFNI CGCG 3 cut(s) 9, 139, 484
BstHHI GCGC 3 cut(s) 9, 11, 141
BstKTI GATC 2 cut(s) 1001, 1235
BstMAI GTCTC 2 cut(s) 44, 163
BstMBI GATC 2 cut(s) 998, 1232
BstMWI GCNNNNNNNGC 6 cut(s) 17, 240, 357, 457, 570, 1028
BstNI CCWGG 2 cut(s) 845, 1012
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 2 cut(s) 843, 1010
BstUI CGCG 3 cut(s) 9, 139, 484
BstV1I GCAGC 2 cut(s) 369, 778
BstXI CCANNNNNNTGG 3 cut(s) 251, 427, 851
Bsu15I ATCGAT 1 cut(s) 224
Bsu36I CCTNAGG 1 cut(s) 1049
BsuRI GGCC 2 cut(s) 256, 296
BsuTUI ATCGAT 1 cut(s) 224
BtsCI GGATG 6 cut(s) 385, 468, 700, 741, 1024, 1060
BtuMI TCGCGA 1 cut(s) 484
Cac8I GCNNGC 2 cut(s) 9, 258
CfoI GCGC 3 cut(s) 9, 11, 141
Cfr10I RCCGGY 2 cut(s) 256, 328
Cfr13I GGNCC 3 cut(s) 254, 545, 597
ClaI ATCGAT 1 cut(s) 224
CseI GACGC 2 cut(s) 26, 26
Csp6I GTAC 3 cut(s) 114, 640, 792
CviAII CATG 5 cut(s) 34, 577, 626, 662, 732
CviQI GTAC 3 cut(s) 114, 640, 792
DdeI CTNAG 5 cut(s) 236, 1032, 1049, 1071, 1197
DpnI GATC 2 cut(s) 1000, 1234
DpnII GATC 2 cut(s) 998, 1232
DraI TTTAAA 1 cut(s) 508
DriI GACNNNNNGTC 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 61, 73, 107, 911
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 4 cut(s) 61, 73, 107, 911
EciI GGCGGA 2 cut(s) 759, 830
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 545, 597
Eco57I CTGAAG 1 cut(s) 1115
Eco81I CCTNAGG 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 843, 1010
EcoT22I ATGCAT 1 cut(s) 667
EcoT38I GRGCYC 1 cut(s) 270
Esp3I CGTCTC 2 cut(s) 44, 163
FaeI CATG 5 cut(s) 37, 580, 629, 665, 735
FatI CATG 5 cut(s) 33, 576, 625, 661, 731
Fnu4HI GCNGC 4 cut(s) 5, 137, 358, 767
FokI GGATG 6 cut(s) 392, 455, 707, 748, 1031, 1067
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 5, 137, 358, 767
FspBI CTAG 4 cut(s) 128, 423, 549, 1083
GlaI GCGC 3 cut(s) 8, 10, 140
GluI GCNGC 4 cut(s) 5, 137, 358, 767
GsaI CCCAGC 2 cut(s) 136, 234
HaeIII GGCC 2 cut(s) 256, 296
HapII CCGG 5 cut(s) 257, 302, 329, 398, 1150
HgaI GACGC 2 cut(s) 26, 26
HhaI GCGC 3 cut(s) 9, 11, 141
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 5 cut(s) 37, 580, 629, 665, 735
Hin6I GCGC 3 cut(s) 7, 9, 139
HinP1I GCGC 3 cut(s) 7, 9, 139
HindIII AAGCTT 2 cut(s) 439, 980
HinfI GANTC 2 cut(s) 226, 973
HpaII CCGG 5 cut(s) 257, 302, 329, 398, 1150
HphI GGTGA 1 cut(s) 718
Hpy166II GTNNAC 2 cut(s) 623, 870
Hpy188I TCNGA 9 cut(s) 123, 239, 343, 404, 532, 1003, 1035, 1074, 1159
Hpy188III TCNNGA 8 cut(s) 28, 483, 620, 669, 827, 884, 1088, 1133
Hpy8I GTNNAC 2 cut(s) 623, 870
Hpy99I CGWCG 3 cut(s) 295, 356, 484
HpyAV CCTTC 6 cut(s) 70, 87, 204, 734, 940, 1009
HpyCH4III ACNGT 1 cut(s) 569
HpyCH4IV ACGT 2 cut(s) 479, 837
HpyCH4V TGCA 4 cut(s) 665, 766, 863, 1184
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 240, 357, 457, 570, 1028
HpyF3I CTNAG 5 cut(s) 236, 1032, 1049, 1071, 1197
HpySE526I ACGT 2 cut(s) 479, 837
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 5 cut(s) 37, 580, 629, 665, 735
HspAI GCGC 3 cut(s) 7, 9, 139
KroI GCCGGC 1 cut(s) 256
KroNI GCCGGC 1 cut(s) 258
Kzo9I GATC 2 cut(s) 998, 1232
LmnI GCTCC 6 cut(s) 146, 344, 448, 517, 561, 1111
Lsp1109I GCAGC 2 cut(s) 369, 778
LweI GCATC 2 cut(s) 674, 1009
MaeI CTAG 4 cut(s) 128, 423, 549, 1083
MaeII ACGT 2 cut(s) 479, 837
MalI GATC 2 cut(s) 1000, 1234
MboI GATC 2 cut(s) 998, 1232
MhlI GDGCHC 3 cut(s) 270, 341, 1116
Mph1103I ATGCAT 1 cut(s) 667
MroNI GCCGGC 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 8 cut(s) 507, 554, 612, 879, 909, 978, 1067, 1095
MslI CAYNNNNRTG 2 cut(s) 425, 734
MspCI CTTAAG 1 cut(s) 977
MspI CCGG 5 cut(s) 257, 302, 329, 398, 1150
MspR9I CCNGG 2 cut(s) 845, 1012
MvaI CCWGG 2 cut(s) 845, 1012
MvnI CGCG 3 cut(s) 9, 139, 484
MwoI GCNNNNNNNGC 6 cut(s) 17, 240, 357, 457, 570, 1028
NaeI GCCGGC 1 cut(s) 258
NdeII GATC 2 cut(s) 998, 1232
NgoMIV GCCGGC 1 cut(s) 256
NlaIII CATG 5 cut(s) 37, 580, 629, 665, 735
NlaIV GGNNCC 2 cut(s) 546, 598
NruI TCGCGA 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 1 cut(s) 665
OliI CACNNNNGTG 1 cut(s) 425
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PdiI GCCGGC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 2 cut(s) 226, 973
PkrI GCNGC 4 cut(s) 6, 138, 359, 768
PpuMI RGGWCCY 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 879
Psp5II RGGWCCY 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 843, 1010
PspFI CCCAGC 2 cut(s) 132, 230
PspGI CCWGG 2 cut(s) 843, 1010
PspN4I GGNNCC 2 cut(s) 546, 598
PspPI GGNCC 3 cut(s) 254, 545, 597
PspPPI RGGWCCY 1 cut(s) 545
PteI GCGCGC 1 cut(s) 7
RruI TCGCGA 1 cut(s) 484
RsaI GTAC 3 cut(s) 115, 641, 793
RsaNI GTAC 3 cut(s) 114, 640, 792
RseI CAYNNNNRTG 2 cut(s) 425, 734
SaqAI TTAA 8 cut(s) 507, 554, 612, 879, 909, 978, 1067, 1095
SatI GCNGC 4 cut(s) 5, 137, 358, 767
Sau3AI GATC 2 cut(s) 998, 1232
Sau96I GGNCC 3 cut(s) 254, 545, 597
ScrFI CCNGG 2 cut(s) 845, 1012
SduI GDGCHC 3 cut(s) 270, 341, 1116
SfaNI GCATC 2 cut(s) 674, 1009
SgsI GGCGCGCC 1 cut(s) 7
SinI GGWCC 2 cut(s) 545, 597
SmiMI CAYNNNNRTG 2 cut(s) 425, 734
SmlI CTYRAG 2 cut(s) 26, 977
SmoI CTYRAG 2 cut(s) 26, 977
SsiI CCGC 5 cut(s) 4, 137, 280, 744, 841
SspI AATATT 2 cut(s) 394, 1120
SspMI CTAG 4 cut(s) 128, 423, 549, 1083
StyD4I CCNGG 2 cut(s) 843, 1010
TaaI ACNGT 1 cut(s) 569
TaiI ACGT 2 cut(s) 482, 840
TaqI TCGA 2 cut(s) 224, 290
TauI GCSGC 2 cut(s) 7, 139
TfiI GAWTC 2 cut(s) 226, 973
Tru1I TTAA 8 cut(s) 507, 554, 612, 879, 909, 978, 1067, 1095
Tru9I TTAA 8 cut(s) 507, 554, 612, 879, 909, 978, 1067, 1095
TseI GCWGC 2 cut(s) 357, 766
TspDTI ATGAA 3 cut(s) 457, 929, 1071
TspGWI ACGGA 4 cut(s) 97, 101, 133, 145
Vha464I CTTAAG 1 cut(s) 977
VpaK11BI GGWCC 2 cut(s) 545, 597
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 6 cut(s) 463, 509, 905, 952, 1058, 1137
XceI RCATGY 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 4 cut(s) 128, 423, 549, 1083
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.