MD01G1169500.v1.1

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006647
Physical Location & Seq
Forward (+)
27334641 .. 27336789
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1169500.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGCTGTGGCGATGGTCTCAGCCGTGAGAGGCGGTCGTTTGCCGTCACTGAACCATCATCACCGCCGTCAGATGATTCTAAGCTCCACGTCGTCGTCGATAATTGGTAGTTGGGGTTCGTTAAAATCATCGAAGCAGCTCATTTGCAGCTCAAACTCAAAGGGTTTCACTCTCTTTTCCCGTTACTCACAAACTCAGGATCTTTACTCCTCCCGCCTTCAGGATAGGTTTGAAGACTTGCCTAGACTGGTGGAGGACATTCTCCAAACATCAGTAAGCACAGGTCCACGTGGCGCTCTTAGGATGGTCCAAGGTATTCAGGCGTTTATTGGGGTTGGTGGGGAGTGGCTTGCTGATGTATCAAAGTCAGCAAACTCATCTGCTGGATTACCAACCCAACTGCAGCTTGGATTGCTGTCCCCCCGTTATTTGAGGAAATTGTTTGAGCGCATGGGGGCAACCTACGTTAAGTTGGGTCAGTTCATTGCATCTGCTCCAACTTTGTTCCCATCAGAATATGTCGAAGAATTTCAGAACTGCTTTGACAGAACTCCTGCAGTACCCTTTGCCGAGATTCAAGCAATCTTGCGTCAGGAATTGGGGAGACCAATTGAAAGCGTTTATGACTATGTTGACCCAACCCCACTCGCTTTGGCCTCAATAGCACAAGTTCATGGTGCAAGGCTAAGAGGCTCCCAAGAAGATGTAGTAATAAAGATCTTGAAACCCGGAATAGAAGATATGTTGGTTGCAGATCTCAATTTTATCTACGTTGTTGTCCGCATTTTGGAATTCTTGAATCCTGATATAAACCGTGCTTCATTGGTATGTGGACATAAATTTCTTCATATGCAACAAATGACTTTAACACTTATACTGGATGACAGGTATAAGCATAGTAATTATTTTCATACTCTTAGAAATAATTTACACTGCTAG

Protein Analysis

313

Amino Acids

34.9

Weight (kDa)

9.03

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 182 - 273 1.2e-21 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 33, 64, 214, 781
AclWI GGATC 1 cut(s) 207
AcsI RAATTY 3 cut(s) 527, 791, 839
AcuI CTGAAG 1 cut(s) 203
AcvI CACGTG 1 cut(s) 290
AfaI GTAC 1 cut(s) 561
AfiI CCNNNNNNNGG 2 cut(s) 220, 787
AgsI TTSAA 5 cut(s) 233, 578, 614, 724, 799
AjiI CACGTC 1 cut(s) 90
AluBI AGCT 4 cut(s) 84, 139, 150, 406
AluI AGCT 4 cut(s) 84, 139, 150, 406
Alw26I GTCTC 2 cut(s) 22, 598
AlwI GGATC 1 cut(s) 207
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 3 cut(s) 136, 147, 403
ApoI RAATTY 3 cut(s) 527, 791, 839
Asp700I GAANNNNTTC 1 cut(s) 528
AspLEI GCGC 2 cut(s) 296, 450
AspS9I GGNCC 2 cut(s) 284, 307
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 1 cut(s) 53
AvaII GGWCC 2 cut(s) 284, 307
BbrPI CACGTG 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 3 cut(s) 148, 159, 415
BccI CCATC 4 cut(s) 7, 63, 298, 517
BceAI ACGGC 3 cut(s) 8, 28, 51
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 2 cut(s) 22, 598
BfaI CTAG 2 cut(s) 243, 937
BfmI CTRYAG 2 cut(s) 401, 555
BfoI RGCGCY 1 cut(s) 297
BglII AGATCT 2 cut(s) 717, 754
BisI GCNGC 3 cut(s) 137, 148, 404
BlsI GCNGC 3 cut(s) 138, 149, 405
Bme1390I CCNGG 1 cut(s) 729
Bme18I GGWCC 2 cut(s) 284, 307
BmgBI CACGTC 1 cut(s) 90
BmgT120I GGNCC 2 cut(s) 284, 307
BmiI GGNNCC 1 cut(s) 694
BmrFI CCNGG 1 cut(s) 729
BmsI GCATC 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 729
BsaAI YACGTR 1 cut(s) 290
BsaI GGTCTC 2 cut(s) 22, 598
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 787
Bse1I ACTGG 2 cut(s) 252, 882
Bse3DI GCAATG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 2 cut(s) 309, 886
BseLI CCNNNNNNNGG 2 cut(s) 220, 787
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 2 cut(s) 33, 209
BseNI ACTGG 2 cut(s) 252, 882
BseRI GAGGAG 1 cut(s) 199
BseXI GCAGC 3 cut(s) 148, 159, 415
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 656
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 729
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 2 cut(s) 220, 787
BsmAI GTCTC 2 cut(s) 22, 598
BsmFI GGGAC 1 cut(s) 403
BsnI GGCC 1 cut(s) 656
Bso31I GGTCTC 2 cut(s) 22, 598
Bsp143I GATC 3 cut(s) 199, 717, 754
BspACI CCGC 4 cut(s) 33, 64, 214, 781
BspANI GGCC 1 cut(s) 656
BspCNI CTCAG 2 cut(s) 32, 208
BspLI GGNNCC 1 cut(s) 694
BspMAI CTGCAG 2 cut(s) 405, 559
BspPI GGATC 1 cut(s) 207
BspTNI GGTCTC 2 cut(s) 22, 598
BsrDI GCAATG 1 cut(s) 483
BsrI ACTGG 2 cut(s) 252, 882
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 3 cut(s) 199, 717, 754
BssT1I CCWWGG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 815
BstBAI YACGTR 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 6 cut(s) 19, 80, 195, 299, 686, 917
BstF5I GGATG 2 cut(s) 309, 886
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 2 cut(s) 296, 450
BstKTI GATC 3 cut(s) 202, 720, 757
BstMAI GTCTC 2 cut(s) 22, 598
BstMBI GATC 3 cut(s) 199, 717, 754
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 2 cut(s) 412, 662
BstSCI CCNGG 1 cut(s) 727
BstSFI CTRYAG 2 cut(s) 401, 555
BstV1I GCAGC 3 cut(s) 148, 159, 415
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 3 cut(s) 199, 717, 754
BstYI RGATCY 3 cut(s) 199, 717, 754
BsuRI GGCC 1 cut(s) 656
BtgZI GCGATG 1 cut(s) 26
BtrI CACGTC 1 cut(s) 90
BtsCI GGATG 2 cut(s) 309, 886
BtsI GCAGTG 1 cut(s) 931
BtsIMutI CAGTG 2 cut(s) 47, 931
Cac8I GCNNGC 1 cut(s) 351
CfoI GCGC 2 cut(s) 296, 450
Cfr13I GGNCC 2 cut(s) 284, 307
CseI GACGC 1 cut(s) 578
Csp6I GTAC 1 cut(s) 560
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviAII CATG 2 cut(s) 451, 674
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 6 cut(s) 19, 80, 195, 299, 686, 917
DpnI GATC 3 cut(s) 201, 719, 756
DpnII GATC 3 cut(s) 199, 717, 754
Eco130I CCWWGG 1 cut(s) 310
Eco31I GGTCTC 2 cut(s) 22, 598
Eco47I GGWCC 2 cut(s) 284, 307
Eco57I CTGAAG 1 cut(s) 203
Eco72I CACGTG 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 791
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 2 cut(s) 454, 677
FaqI GGGAC 1 cut(s) 403
FatI CATG 2 cut(s) 450, 673
FauI CCCGC 1 cut(s) 221
FauNDI CATATG 1 cut(s) 849
Fnu4HI GCNGC 3 cut(s) 137, 148, 404
FokI GGATG 2 cut(s) 316, 893
Fsp4HI GCNGC 3 cut(s) 137, 148, 404
FspBI CTAG 2 cut(s) 243, 937
GlaI GCGC 2 cut(s) 295, 449
GluI GCNGC 3 cut(s) 137, 148, 404
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 1 cut(s) 656
HapII CCGG 1 cut(s) 729
HgaI GACGC 1 cut(s) 578
HhaI GCGC 2 cut(s) 296, 450
Hin1II CATG 2 cut(s) 454, 677
Hin6I GCGC 2 cut(s) 294, 448
HinP1I GCGC 2 cut(s) 294, 448
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 3 cut(s) 76, 574, 799
HpaII CCGG 1 cut(s) 729
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 3 cut(s) 287, 634, 833
Hpy188I TCNGA 3 cut(s) 72, 514, 534
Hpy188III TCNNGA 6 cut(s) 197, 221, 593, 721, 796, 803
Hpy8I GTNNAC 3 cut(s) 287, 634, 833
Hpy99I CGWCG 3 cut(s) 94, 97, 100
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 1 cut(s) 815
HpyCH4IV ACGT 4 cut(s) 89, 289, 465, 771
HpyCH4V TGCA 7 cut(s) 147, 403, 488, 557, 680, 752, 853
HpyF10VI GCNNNNNNNGC 2 cut(s) 412, 662
HpyF3I CTNAG 6 cut(s) 19, 80, 195, 299, 686, 917
HpySE526I ACGT 4 cut(s) 89, 289, 465, 771
Hsp92II CATG 2 cut(s) 454, 677
HspAI GCGC 2 cut(s) 294, 448
Kzo9I GATC 3 cut(s) 199, 717, 754
LmnI GCTCC 3 cut(s) 89, 499, 698
Lsp1109I GCAGC 3 cut(s) 148, 159, 415
LweI GCATC 1 cut(s) 497
MaeI CTAG 2 cut(s) 243, 937
MaeII ACGT 4 cut(s) 89, 289, 465, 771
MaeIII GTNAC 2 cut(s) 45, 182
MalI GATC 3 cut(s) 201, 719, 756
MboI GATC 3 cut(s) 199, 717, 754
MboII GAAGA 5 cut(s) 245, 536, 713, 749, 836
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 3 cut(s) 199, 717, 754
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 6 cut(s) 23, 220, 247, 426, 667, 683
MroXI GAANNNNTTC 1 cut(s) 528
MseI TTAA 3 cut(s) 122, 468, 866
MslI CAYNNNNRTG 1 cut(s) 826
MspI CCGG 1 cut(s) 729
MspR9I CCNGG 1 cut(s) 729
MunI CAATTG 1 cut(s) 609
MwoI GCNNNNNNNGC 2 cut(s) 412, 662
NciI CCSGG 1 cut(s) 729
NdeI CATATG 1 cut(s) 849
NdeII GATC 3 cut(s) 199, 717, 754
NlaIII CATG 2 cut(s) 454, 677
NlaIV GGNNCC 1 cut(s) 694
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 3 cut(s) 76, 574, 799
PkrI GCNGC 3 cut(s) 138, 149, 405
PmaCI CACGTG 1 cut(s) 290
PmlI CACGTG 1 cut(s) 290
Ppu21I YACGTR 1 cut(s) 290
PspCI CACGTG 1 cut(s) 290
PspN4I GGNNCC 1 cut(s) 694
PspPI GGNCC 2 cut(s) 284, 307
PsrI GAACNNNNNNTAC 2 cut(s) 100, 132
PstI CTGCAG 2 cut(s) 405, 559
PsuI RGATCY 3 cut(s) 199, 717, 754
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 826
SaqAI TTAA 3 cut(s) 122, 468, 866
SatI GCNGC 3 cut(s) 137, 148, 404
Sau3AI GATC 3 cut(s) 199, 717, 754
Sau96I GGNCC 2 cut(s) 284, 307
ScrFI CCNGG 1 cut(s) 729
SfaNI GCATC 1 cut(s) 497
SfcI CTRYAG 2 cut(s) 401, 555
SinI GGWCC 2 cut(s) 284, 307
SmiMI CAYNNNNRTG 1 cut(s) 826
SsiI CCGC 4 cut(s) 33, 64, 214, 781
SspMI CTAG 2 cut(s) 243, 937
StyD4I CCNGG 1 cut(s) 727
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 815
TaiI ACGT 4 cut(s) 92, 292, 468, 774
TaqI TCGA 3 cut(s) 98, 131, 522
TfiI GAWTC 3 cut(s) 76, 574, 799
Tru1I TTAA 3 cut(s) 122, 468, 866
Tru9I TTAA 3 cut(s) 122, 468, 866
TscAI CASTG 2 cut(s) 54, 938
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 3 cut(s) 136, 147, 403
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 5 cut(s) 472, 662, 810, 836, 899
TspRI CASTG 2 cut(s) 54, 938
VpaK11BI GGWCC 2 cut(s) 284, 307
XapI RAATTY 3 cut(s) 527, 791, 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 528
XspI CTAG 2 cut(s) 243, 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.