RLG00000026889

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006647
Physical Location & Seq
Reverse (-)
6790318 .. 6796357
6040 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026889

Sequence Viewer

Length: 1644 bp
ATGGCCTTGGTCTCGGCTGTGAGAGGCGGCCGGCTCCCGTCATCATTACATCATCATCATCATCTGGGTTCGTCTTCGTCTTCATCACTAGGTAGTTGGGGTTCGTTAAGACCGTGGAAGCAGATGAATTCTAGCTCAAAGGGCTTCACTCTCTTGGCTCGTTACTCACAAACACAGGACCTTTTCTCCTCTCGCCTTCAAGATAGTATGGAAAACTTGCCTAAACTGGTGGAGGATATTGTCCGGACATCATTAAGTACAGGCCCACGTGGCGCCCTAAGGATGGCTCAAGGTATTCAAGCATTTATTGGTGTTGGTGGGGAGTGGCTTACTGAGGCATCCAAGTCAGCAAATTCATCTACTGGCTTACCAACCCAATTGCAGCTTGGAGTGCTCTCCCCCCTTTACTTGAGGAGATTGTTTGAGCGCATGGGAGCAACTTATATTAAATTGGGCCAGTTCATTGCATCCACACCATCATTGTTCCCAGCAGACTATGTCGAGGAATTTCAGAACTGCTTTGACAGAGCTCCCGCAATACCTTTTGTGGAGATTCAACAAATATTGCGCCAGGAATTGGGGAGACCAATTGAAAGTGTATTTGAGTATGTTGACCCAACTCCAATTGCTTCGGCCTCAATAGCACAAGTTCACGGTGCAAGGCTAAGAGGGTCCCAAGAAGATGTGGTTATAAAGGTCTTGAAACCCGGAATAGAAGATATGTTGGTTGCAGATCTGAATTTTATTTACATAGTTGCTCGCATTTTGGAGTTCCTGAGTCCTGACATAAGCCGTGCTTCACTGGTTGGTATCGTGAAGGACATACGGGAATCTATGCTTGAAGAGGTTGATTTCTACAAGGAGGCTGCAAATGTTGAGTCCTTCAGGAGATATTTAGAAGCCATGGGACTCACAAGGCAGGCTACAGCTCCAAGAGTGTATCACCAATGCAGCACAAAACGAATTTTAACAATGGAGAGGCTCTATGGGGTTCCTCTTACTGACTTAGACTCTATAAGTTCACTTGTTTCTAGTCCGGAGGCCAGTCTCATAACTGCCCTTAATGTGTGGTTTGGCAGTTTACTTGCCTGTGAAAGCTTCCATGCAGATGTGCATGCTGGAAATCTGTGGTTGTTGCGTGATGGCCGTATTGGGTTTCTTGACTTTGGAATCGTAGGTCGTATAGACCCACAAACATGGGCTGCCATGGAAATCTTTTTGGCATCAATTGCAACTGAAGAATATGAATCAATGGCATCTGCGTTGATTCAAATGGGTGCTACAGACACAAATGTTGATGCTCAGGCCTTTGCAAGAGACTTGGAAAAAATGTTCTCATCAATGAAGGAGTTGGATACGGAAATAGTCATAGCAACAGCCAGGGAGCCGGCTACAAATGCAACTGCTGTTTCTGCTAATGTAGTCGTTGATGAGAGACAAATGAATGCACTTTTTCTTGATGTGGTTCGGGTTAGTGAATCATATGGATTGAAGTTTCCTCGAGAGTTTGCACTTCTCTTGAAGCAGCTCTTGTATTTTGATCGGTACACCCGTTTGTTGGCCCCAAACTTGAATATGCTCCAGGACCAAAGGATTTCAATTGCTTCAAATCGAAGACGCAACTACAGGGACAATTTCAGCTGA

Protein Analysis

548

Amino Acids

60.91

Weight (kDa)

5.83

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 174 - 420 2.3e-59 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 577
AccIII TCCGGA 2 cut(s) 243, 1036
AciI CCGC 2 cut(s) 27, 534
AcoI YGGCCR 2 cut(s) 28, 1144
AcsI RAATTY 5 cut(s) 127, 352, 506, 739, 963
AcuI CTGAAG 2 cut(s) 868, 1257
AcvI CACGTG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 2 cut(s) 259, 1547
AfiI CCNNNNNNNGG 3 cut(s) 283, 577, 1558
AjnI CCWGG 3 cut(s) 570, 1379, 1581
AloI GAACNNNNNNTCC 2 cut(s) 1316, 1348
AluBI AGCT 7 cut(s) 135, 385, 530, 929, 1098, 1528, 1641
AluI AGCT 7 cut(s) 135, 385, 530, 929, 1098, 1528, 1641
Alw21I GWGCWC 2 cut(s) 396, 532
Alw26I GTCTC 5 cut(s) 16, 577, 1052, 1311, 1429
Ama87I CYCGRG 1 cut(s) 1500
Aor13HI TCCGGA 2 cut(s) 243, 1036
AoxI GGCC 9 cut(s) 3, 28, 262, 454, 633, 1041, 1144, 1305, 1560
ApeKI GCWGC 5 cut(s) 382, 866, 951, 1202, 1525
ApoI RAATTY 5 cut(s) 127, 352, 506, 739, 963
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AspLEI GCGC 3 cut(s) 275, 429, 570
AspS9I GGNCC 6 cut(s) 178, 263, 454, 672, 1561, 1585
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 1 cut(s) 935
AvaI CYCGRG 1 cut(s) 1500
AvaII GGWCC 3 cut(s) 178, 672, 1585
AxyI CCTNAGG 1 cut(s) 278
BaeI ACNNNNGTAYC 2 cut(s) 1537, 1570
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 532
BbrPI CACGTG 1 cut(s) 269
BbsI GAAGAC 3 cut(s) 66, 72, 1621
Bbv12I GWGCWC 2 cut(s) 396, 532
BbvI GCAGC 5 cut(s) 394, 853, 963, 1189, 1537
BccI CCATC 3 cut(s) 277, 484, 1136
BceAI ACGGC 2 cut(s) 777, 1131
BciT130I CCWGG 3 cut(s) 572, 1381, 1583
BciVI GTATCC 1 cut(s) 1348
BcnI CCSGG 1 cut(s) 708
BcoDI GTCTC 5 cut(s) 16, 577, 1052, 1311, 1429
BfaI CTAG 3 cut(s) 89, 132, 1032
BfmI CTRYAG 3 cut(s) 924, 1281, 1624
BfoI RGCGCY 1 cut(s) 276
BfuI GTATCC 1 cut(s) 1348
BglI GCCNNNNNGGC 1 cut(s) 270
BglII AGATCT 1 cut(s) 733
BisI GCNGC 6 cut(s) 28, 383, 867, 952, 1203, 1526
BlsI GCNGC 6 cut(s) 29, 384, 868, 953, 1204, 1527
Bme1390I CCNGG 4 cut(s) 572, 708, 1381, 1583
Bme18I GGWCC 3 cut(s) 178, 672, 1585
BmeT110I CYCGRG 1 cut(s) 1500
BmgT120I GGNCC 6 cut(s) 178, 263, 454, 672, 1561, 1585
BmiI GGNNCC 7 cut(s) 35, 274, 673, 674, 993, 1386, 1563
BmrFI CCNGG 4 cut(s) 572, 708, 1381, 1583
BmsI GCATC 5 cut(s) 347, 476, 1232, 1265, 1288
BpiI GAAGAC 3 cut(s) 66, 72, 1621
BpmI CTGGAG 1 cut(s) 1565
Bpu10I CCTNAGC 1 cut(s) 1302
BpuEI CTTGAG 2 cut(s) 273, 430
BpuMI CCSGG 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 2 cut(s) 16, 577
BsaJI CCNNGG 5 cut(s) 6, 113, 903, 1206, 1380
BsaWI WCCGGW 2 cut(s) 243, 1036
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 577, 1558
Bse118I RCCGGY 2 cut(s) 30, 1387
Bse1I ACTGG 5 cut(s) 231, 367, 457, 807, 1044
Bse21I CCTNAGG 1 cut(s) 278
Bse3DI GCAATG 1 cut(s) 462
BseAI TCCGGA 2 cut(s) 243, 1036
BseBI CCWGG 3 cut(s) 572, 1381, 1583
BseDI CCNNGG 5 cut(s) 6, 113, 903, 1206, 1380
BseGI GGATG 3 cut(s) 288, 338, 467
BseLI CCNNNNNNNGG 3 cut(s) 283, 577, 1558
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 3 cut(s) 324, 767, 1316
BseNI ACTGG 5 cut(s) 231, 367, 457, 807, 1044
BseRI GAGGAG 2 cut(s) 178, 427
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 5 cut(s) 394, 853, 963, 1189, 1537
BseYI CCCAGC 1 cut(s) 487
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 9 cut(s) 5, 30, 264, 456, 635, 1043, 1146, 1307, 1562
BshNI GGYRCC 1 cut(s) 272
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 2 cut(s) 396, 532
BsiHKCI CYCGRG 1 cut(s) 1500
BsiSI CCGG 5 cut(s) 31, 244, 708, 1037, 1388
BslFI GGGAC 2 cut(s) 658, 921
BslI CCNNNNNNNGG 3 cut(s) 283, 577, 1558
BsmAI GTCTC 5 cut(s) 16, 577, 1052, 1311, 1429
BsmFI GGGAC 2 cut(s) 658, 921
BsmI GAATGC 1 cut(s) 1450
BsnI GGCC 9 cut(s) 5, 30, 264, 456, 635, 1043, 1146, 1307, 1562
Bso31I GGTCTC 2 cut(s) 16, 577
BsoBI CYCGRG 1 cut(s) 1500
Bsp1286I GDGCHC 2 cut(s) 396, 532
Bsp13I TCCGGA 2 cut(s) 243, 1036
Bsp143I GATC 2 cut(s) 733, 1540
Bsp19I CCATGG 2 cut(s) 903, 1206
BspACI CCGC 2 cut(s) 27, 534
BspANI GGCC 9 cut(s) 5, 30, 264, 456, 635, 1043, 1146, 1307, 1562
BspCNI CTCAG 3 cut(s) 325, 768, 1315
BspEI TCCGGA 2 cut(s) 243, 1036
BspLI GGNNCC 7 cut(s) 35, 274, 673, 674, 993, 1386, 1563
BspT107I GGYRCC 1 cut(s) 272
BspTNI GGTCTC 2 cut(s) 16, 577
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 2 cut(s) 30, 1387
BsrI ACTGG 5 cut(s) 231, 367, 457, 807, 1044
BssAI RCCGGY 2 cut(s) 30, 1387
BssECI CCNNGG 5 cut(s) 6, 113, 903, 1206, 1380
BssMI GATC 2 cut(s) 733, 1540
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 3 cut(s) 6, 903, 1206
Bst2UI CCWGG 3 cut(s) 572, 1381, 1583
Bst4CI ACNGT 2 cut(s) 114, 656
Bst6I CTCTTC 1 cut(s) 837
BstACI GRCGYC 1 cut(s) 273
BstAPI GCANNNNNTGC 1 cut(s) 1229
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 5 cut(s) 32, 760, 921, 1116, 1389
BstDEI CTNAG 6 cut(s) 278, 333, 665, 776, 1006, 1302
BstDSI CCRYGG 3 cut(s) 113, 903, 1206
BstF5I GGATG 3 cut(s) 288, 338, 467
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 3 cut(s) 275, 429, 570
BstKTI GATC 2 cut(s) 736, 1543
BstMAI GTCTC 5 cut(s) 16, 577, 1052, 1311, 1429
BstMBI GATC 2 cut(s) 733, 1540
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 7 cut(s) 141, 270, 391, 641, 1229, 1397, 1412
BstNI CCWGG 3 cut(s) 572, 1381, 1583
BstNSI RCATGY 1 cut(s) 1118
BstSCI CCNGG 4 cut(s) 570, 706, 1379, 1581
BstSFI CTRYAG 3 cut(s) 924, 1281, 1624
BstV1I GCAGC 5 cut(s) 394, 853, 963, 1189, 1537
BstV2I GAAGAC 3 cut(s) 66, 72, 1621
BstX2I RGATCY 1 cut(s) 733
BstXI CCANNNNNNTGG 1 cut(s) 1197
BstYI RGATCY 1 cut(s) 733
BstZI CGGCCG 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 278
BsuI GTATCC 1 cut(s) 1348
BsuRI GGCC 9 cut(s) 5, 30, 264, 456, 635, 1043, 1146, 1307, 1562
BtgI CCRYGG 3 cut(s) 113, 903, 1206
BtsCI GGATG 3 cut(s) 288, 338, 467
BtsIMutI CAGTG 1 cut(s) 800
Cac8I GCNNGC 5 cut(s) 32, 760, 921, 1116, 1389
CfoI GCGC 3 cut(s) 275, 429, 570
Cfr10I RCCGGY 2 cut(s) 30, 1387
Cfr13I GGNCC 6 cut(s) 178, 263, 454, 672, 1561, 1585
CseI GACGC 1 cut(s) 1626
Csp6I GTAC 2 cut(s) 258, 1546
CviAII CATG 6 cut(s) 430, 904, 1103, 1115, 1197, 1207
CviQI GTAC 2 cut(s) 258, 1546
DdeI CTNAG 6 cut(s) 278, 333, 665, 776, 1006, 1302
DinI GGCGCC 1 cut(s) 274
DpnI GATC 2 cut(s) 735, 1542
DpnII GATC 2 cut(s) 733, 1540
EaeI YGGCCR 2 cut(s) 28, 1144
EagI CGGCCG 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 837
EarI CTCTTC 1 cut(s) 837
Ecl136II GAGCTC 1 cut(s) 530
EclXI CGGCCG 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 6, 903, 1206
Eco147I AGGCCT 1 cut(s) 1307
Eco24I GRGCYC 1 cut(s) 532
Eco31I GGTCTC 2 cut(s) 16, 577
Eco47I GGWCC 3 cut(s) 178, 672, 1585
Eco52I CGGCCG 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 530
Eco57I CTGAAG 2 cut(s) 868, 1257
Eco72I CACGTG 1 cut(s) 269
Eco81I CCTNAGG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 1500
EcoICRI GAGCTC 1 cut(s) 530
EcoO109I RGGNCCY 2 cut(s) 178, 672
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 3 cut(s) 570, 1379, 1581
EcoT14I CCWWGG 3 cut(s) 6, 903, 1206
EcoT38I GRGCYC 1 cut(s) 532
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 3 cut(s) 6, 903, 1206
FaeI CATG 6 cut(s) 433, 907, 1106, 1118, 1200, 1210
FalI AAGNNNNNCTT 4 cut(s) 781, 813, 1514, 1546
FaqI GGGAC 2 cut(s) 658, 921
FatI CATG 6 cut(s) 429, 903, 1102, 1114, 1196, 1206
FauI CCCGC 1 cut(s) 541
FauNDI CATATG 1 cut(s) 1483
Fnu4HI GCNGC 6 cut(s) 28, 383, 867, 952, 1203, 1526
FokI GGATG 3 cut(s) 295, 325, 454
FriOI GRGCYC 1 cut(s) 532
Fsp4HI GCNGC 6 cut(s) 28, 383, 867, 952, 1203, 1526
FspBI CTAG 3 cut(s) 89, 132, 1032
GlaI GCGC 3 cut(s) 274, 428, 569
GluI GCNGC 6 cut(s) 28, 383, 867, 952, 1203, 1526
GsaI CCCAGC 1 cut(s) 491
GsuI CTGGAG 1 cut(s) 1565
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 9 cut(s) 5, 30, 264, 456, 635, 1043, 1146, 1307, 1562
HapII CCGG 5 cut(s) 31, 244, 708, 1037, 1388
HgaI GACGC 1 cut(s) 1626
HhaI GCGC 3 cut(s) 275, 429, 570
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 6 cut(s) 433, 907, 1106, 1118, 1200, 1210
Hin6I GCGC 3 cut(s) 273, 427, 568
HinP1I GCGC 3 cut(s) 273, 427, 568
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HindIII AAGCTT 1 cut(s) 1096
HpaII CCGG 5 cut(s) 31, 244, 708, 1037, 1388
HphI GGTGA 1 cut(s) 935
Hpy166II GTNNAC 5 cut(s) 613, 652, 1022, 1082, 1548
Hpy188I TCNGA 2 cut(s) 513, 738
Hpy8I GTNNAC 5 cut(s) 613, 652, 1022, 1082, 1548
HpyAV CCTTC 4 cut(s) 206, 811, 892, 1339
HpyCH4III ACNGT 2 cut(s) 114, 656
HpyCH4IV ACGT 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 7 cut(s) 141, 270, 391, 641, 1229, 1397, 1412
HpyF3I CTNAG 6 cut(s) 278, 333, 665, 776, 1006, 1302
HpySE526I ACGT 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 6 cut(s) 433, 907, 1106, 1118, 1200, 1210
HspAI GCGC 3 cut(s) 273, 427, 568
KasI GGCGCC 1 cut(s) 272
KflI GGGWCCC 1 cut(s) 672
Kpn2I TCCGGA 2 cut(s) 243, 1036
KroI GCCGGC 2 cut(s) 30, 1387
KroNI GCCGGC 2 cut(s) 32, 1389
Kzo9I GATC 2 cut(s) 733, 1540
LmnI GCTCC 6 cut(s) 39, 434, 535, 934, 1384, 1584
Lsp1109I GCAGC 5 cut(s) 394, 853, 963, 1189, 1537
LweI GCATC 5 cut(s) 347, 476, 1232, 1265, 1288
MaeI CTAG 3 cut(s) 89, 132, 1032
MaeII ACGT 1 cut(s) 268
MaeIII GTNAC 1 cut(s) 161
MalI GATC 2 cut(s) 735, 1542
MboI GATC 2 cut(s) 733, 1540
MboII GAAGA 7 cut(s) 66, 72, 692, 728, 854, 1250, 1626
MfeI CAATTG 5 cut(s) 377, 588, 624, 1227, 1599
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 2 cut(s) 396, 532
Mly113I GGCGCC 1 cut(s) 273
MlyI GAGTC 4 cut(s) 787, 887, 903, 1004
MmeI TCCRAC 1 cut(s) 1332
MroI TCCGGA 2 cut(s) 243, 1036
MroNI GCCGGC 2 cut(s) 30, 1387
MseI TTAA 5 cut(s) 107, 254, 447, 968, 1062
MslI CAYNNNNRTG 2 cut(s) 1107, 1195
MspA1I CMGCKG 1 cut(s) 1641
MspI CCGG 5 cut(s) 31, 244, 708, 1037, 1388
MspR9I CCNGG 4 cut(s) 572, 708, 1381, 1583
MunI CAATTG 5 cut(s) 377, 588, 624, 1227, 1599
Mva1269I GAATGC 1 cut(s) 1450
MvaI CCWGG 3 cut(s) 572, 1381, 1583
MwoI GCNNNNNNNGC 7 cut(s) 141, 270, 391, 641, 1229, 1397, 1412
NaeI GCCGGC 2 cut(s) 32, 1389
NarI GGCGCC 1 cut(s) 273
NciI CCSGG 1 cut(s) 708
NcoI CCATGG 2 cut(s) 903, 1206
NdeI CATATG 1 cut(s) 1483
NdeII GATC 2 cut(s) 733, 1540
NgoMIV GCCGGC 2 cut(s) 30, 1387
NlaIII CATG 6 cut(s) 433, 907, 1106, 1118, 1200, 1210
NlaIV GGNNCC 7 cut(s) 35, 274, 673, 674, 993, 1386, 1563
NspI RCATGY 1 cut(s) 1118
PaeI GCATGC 1 cut(s) 1118
PaeR7I CTCGAG 1 cut(s) 1500
PceI AGGCCT 1 cut(s) 1307
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 1549
PctI GAATGC 1 cut(s) 1450
PdiI GCCGGC 2 cut(s) 32, 1389
PfeI GAWTC 6 cut(s) 553, 830, 1170, 1247, 1267, 1478
PflFI GACNNNGTC 1 cut(s) 497
PflMI CCANNNNNTGG 1 cut(s) 577
PfoI TCCNGGA 1 cut(s) 1581
PkrI GCNGC 6 cut(s) 29, 384, 868, 953, 1204, 1527
PleI GAGTC 4 cut(s) 786, 886, 903, 1004
PluTI GGCGCC 1 cut(s) 276
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 4 cut(s) 786, 886, 903, 1004
Ppu21I YACGTR 1 cut(s) 269
PpuMI RGGWCCY 2 cut(s) 178, 672
PsiI TTATAA 1 cut(s) 692
Psp124BI GAGCTC 1 cut(s) 532
Psp5II RGGWCCY 2 cut(s) 178, 672
Psp6I CCWGG 3 cut(s) 570, 1379, 1581
PspCI CACGTG 1 cut(s) 269
PspFI CCCAGC 1 cut(s) 487
PspGI CCWGG 3 cut(s) 570, 1379, 1581
PspN4I GGNNCC 7 cut(s) 35, 274, 673, 674, 993, 1386, 1563
PspPI GGNCC 6 cut(s) 178, 263, 454, 672, 1561, 1585
PspPPI RGGWCCY 2 cut(s) 178, 672
PsrI GAACNNNNNNTAC 2 cut(s) 85, 117
PsuI RGATCY 1 cut(s) 733
PsyI GACNNNGTC 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 1641
RsaI GTAC 2 cut(s) 259, 1547
RsaNI GTAC 2 cut(s) 258, 1546
RseI CAYNNNNRTG 2 cut(s) 1107, 1195
SacI GAGCTC 1 cut(s) 532
SaqAI TTAA 5 cut(s) 107, 254, 447, 968, 1062
SatI GCNGC 6 cut(s) 28, 383, 867, 952, 1203, 1526
Sau3AI GATC 2 cut(s) 733, 1540
Sau96I GGNCC 6 cut(s) 178, 263, 454, 672, 1561, 1585
SchI GAGTC 4 cut(s) 787, 887, 903, 1004
ScrFI CCNGG 4 cut(s) 572, 708, 1381, 1583
SduI GDGCHC 2 cut(s) 396, 532
SfaNI GCATC 5 cut(s) 347, 476, 1232, 1265, 1288
SfcI CTRYAG 3 cut(s) 924, 1281, 1624
SfoI GGCGCC 1 cut(s) 274
Sfr274I CTCGAG 1 cut(s) 1500
SinI GGWCC 3 cut(s) 178, 672, 1585
SlaI CTCGAG 1 cut(s) 1500
SmiMI CAYNNNNRTG 2 cut(s) 1107, 1195
SmlI CTYRAG 3 cut(s) 288, 409, 1500
SmoI CTYRAG 3 cut(s) 288, 409, 1500
SphI GCATGC 1 cut(s) 1118
SseBI AGGCCT 1 cut(s) 1307
SsiI CCGC 2 cut(s) 27, 534
SspDI GGCGCC 1 cut(s) 272
SspI AATATT 1 cut(s) 564
SspMI CTAG 3 cut(s) 89, 132, 1032
SstI GAGCTC 1 cut(s) 532
StuI AGGCCT 1 cut(s) 1307
StyD4I CCNGG 4 cut(s) 570, 706, 1379, 1581
StyI CCWWGG 3 cut(s) 6, 903, 1206
TaaI ACNGT 2 cut(s) 114, 656
TaiI ACGT 1 cut(s) 271
TaqI TCGA 3 cut(s) 501, 1501, 1612
TatI WGTACW 1 cut(s) 257
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 6 cut(s) 553, 830, 1170, 1247, 1267, 1478
Tru1I TTAA 5 cut(s) 107, 254, 447, 968, 1062
Tru9I TTAA 5 cut(s) 107, 254, 447, 968, 1062
TscAI CASTG 1 cut(s) 807
TseI GCWGC 5 cut(s) 382, 866, 951, 1202, 1525
TspDTI ATGAA 7 cut(s) 72, 140, 345, 451, 1260, 1358, 1457
TspGWI ACGGA 1 cut(s) 1373
TspRI CASTG 1 cut(s) 807
Tth111I GACNNNGTC 1 cut(s) 497
Van91I CCANNNNNTGG 1 cut(s) 577
VpaK11BI GGWCC 3 cut(s) 178, 672, 1585
XapI RAATTY 5 cut(s) 127, 352, 506, 739, 963
XceI RCATGY 1 cut(s) 1118
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XhoI CTCGAG 1 cut(s) 1500
XspI CTAG 3 cut(s) 89, 132, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.