MD07G1235400.v1.1

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006647
Physical Location & Seq
Forward (+)
30846587 .. 30851126
4540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1235400.v1.1.491

Sequence Viewer

Length: 1674 bp
ATGGCTGTTTCGATGGTCTCAGCCGTGAGAGGTGGTCGTTTGCCGGCACTTCACCGTCATCACCGCCATCACCGCCGTCAGATGATTCCGAGCTCCTCCTCCTCGTCGATTATTGGTAGTTGGGGTTCGTTAAAATCATCGAAGCAGCTTAATTGCAGCTCAAACTCAAGGGGCTTCACTCTCTTTGCCCGGTACTCTCAAGCTCAGGATCTTTACTCCTCCCGCCTTCAAGATAGGTTCGAAGACTTGCCTAAACTGGTGGAGGACATTCTCCAAACATCAGTAAGCACAGGTCCTCGCGGCGCTCTTAGGATGGTCCAAGGTATTCAGGCGTTTATTGGGGTTGGGGGGGAGTGGCTTGCCGACGTATCAAAGTCAGCAAACTCATCTGCCGGATTACCAACCCAACTGCAGCTTGGATTGGTGTCCCCCCTTTATTTGAGGAGATTGTTTGAGCGCATGGGGGCAACCTACGTTAAGTTGGGTCAGTTCATTGCATCGGCACCAACATTGTTCCCACCAGAATATGTTGAAGAATTTCAGAACTGCTTTGACAGAACTCCTGCAGTACCCTTTGCGGAGATTCAAGCAATCTTGCGACAGGAATTGGGGAGACCAATTGACAGTGTTTATGAATATGTTGACCCAACCCCGCTCGCTTCAGCCTCAATAGCACAAGTTCATGCTGCAAGGCTAAGAGGCTCCCAAGAAGATGTAGTTATAAAGATCTTGAAACCCGGAATAGAAGATATGTTGGTTGCAGATCTAAATTTTATCTACATTGTAGTCCGCATTTTGGAATTCTTGAGTCCTGACATAAGCCGCGCTTCATTGGTTGGTATTGTGCAAGATATACGGGAGTCCATGCTTGAAGAAGTTGATTTCTATAAGGAGGCTGCAAACATTGAGTCCTTTAGGAGATATTTAGAAGCCATGGGACTCACAAGGCAGGCTACGGCTCCAAAAGTGTATCACCAATGCAGCAGCAAACGAGTTTTAACAATGGAGAGGCTATACGGAGTTCCTCTTACTGACTTAAACAGTATAAGTTCCTTTGTCTCTAATCCGGAGACCAGTCTCATAACTGCCCTTAATGTGTGGTTTGGCAGTTTGCTTGCCTGTGAAAGCTTCCACGCAGATGTGCATGCAGGCAACCTGTGGTTGTTGCGAGATGGCCGCATTGGGTTTCTTGATTTTGGAATCGTTGGCCGTATATCCCCGAAAACATGGGGTGCCATGGAAATCTTTCTGGCATCAATTGCAACTGAAGAATATGAATCAATGGCATCTGCACTGATTGAAATGGGTGCTACAGACACAAATGTTGATACTAAGGCCTTTGCAAGAGACTTGGAAAAGATGTTCTCATCAATAAAGGATTTGGATACTGAAGTAGTCATAGCAACTGCCAGGGAACCATCTACGAATGCAACTGCTGTCTCTGCTAATCTAGTTGTTGATGAGAGACAAATGAATGCACTTTTTCTCGATGTGGTTCGGGTTAGTGAATCATATGGATTGAAATTTCCACGAGAGTTTGCACTTCTCCTGAAGCAGCTTTTGTATTTTGATCGGTACACCCGTTTGTTGGCCCCGAACTTGAATATGCTCCAAGACCAAAGGATTTCCATCGCTTCTAATCGAAGAACCAACTACAGGAACAATTTCAGATGA

Protein Analysis

558

Amino Acids

62.24

Weight (kDa)

6.78

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 185 - 430 3.2e-58 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 722
AccB1I GGYRCC 2 cut(s) 502, 1232
AccBSI CCGCTC 1 cut(s) 655
AccII CGCG 2 cut(s) 300, 825
AccIII TCCGGA 1 cut(s) 1066
AciI CCGC 9 cut(s) 64, 73, 223, 300, 578, 653, 790, 823, 1177
AclWI GGATC 1 cut(s) 216
AcoI YGGCCR 2 cut(s) 1174, 1207
AcsI RAATTY 4 cut(s) 536, 769, 800, 1523
AcuI CTGAAG 4 cut(s) 645, 1287, 1410, 1571
AfaI GTAC 3 cut(s) 194, 570, 1577
AfiI CCNNNNNNNGG 3 cut(s) 796, 1588, 1656
AgsI TTSAA 8 cut(s) 230, 533, 587, 733, 872, 1301, 1522, 1603
AjnI CCWGG 1 cut(s) 1409
AloI GAACNNNNNNTCC 2 cut(s) 1346, 1378
AluBI AGCT 7 cut(s) 93, 148, 159, 203, 415, 1128, 1558
AluI AGCT 7 cut(s) 93, 148, 159, 203, 415, 1128, 1558
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 8 cut(s) 22, 607, 1063, 1064, 1082, 1341, 1444, 1459
AlwI GGATC 1 cut(s) 216
Aor13HI TCCGGA 1 cut(s) 1066
AoxI GGCC 4 cut(s) 1174, 1207, 1335, 1590
ApeKI GCWGC 8 cut(s) 145, 156, 412, 686, 896, 981, 984, 1555
ApoI RAATTY 4 cut(s) 536, 769, 800, 1523
ArsI GACNNNNNNTTYG 2 cut(s) 893, 925
Asp700I GAANNNNTTC 3 cut(s) 537, 1245, 1664
AspLEI GCGC 3 cut(s) 305, 459, 827
AspS9I GGNCC 3 cut(s) 293, 316, 1591
AsuC2I CCSGG 2 cut(s) 190, 738
AsuHPI GGTGA 4 cut(s) 44, 53, 62, 965
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 2 cut(s) 293, 316
BaeI ACNNNNGTAYC 4 cut(s) 1377, 1410, 1567, 1600
BanI GGYRCC 2 cut(s) 502, 1232
BanII GRGCYC 1 cut(s) 95
BauI CACGAG 1 cut(s) 1530
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 8 cut(s) 157, 168, 424, 673, 883, 993, 996, 1567
BccI CCATC 6 cut(s) 7, 75, 307, 1166, 1426, 1637
BceAI ACGGC 4 cut(s) 8, 60, 972, 1194
BciT130I CCWGG 1 cut(s) 1411
BciVI GTATCC 1 cut(s) 1378
BcnI CCSGG 2 cut(s) 190, 738
BcoDI GTCTC 8 cut(s) 22, 607, 1063, 1064, 1082, 1341, 1444, 1459
BfaI CTAG 1 cut(s) 1451
BfmI CTRYAG 4 cut(s) 410, 564, 1311, 1654
BfoI RGCGCY 1 cut(s) 306
BfuI GTATCC 1 cut(s) 1378
BglII AGATCT 2 cut(s) 726, 763
Bme1390I CCNGG 3 cut(s) 190, 738, 1411
Bme18I GGWCC 2 cut(s) 293, 316
BmgT120I GGNCC 3 cut(s) 293, 316, 1591
BmiI GGNNCC 6 cut(s) 504, 703, 960, 1234, 1416, 1593
BmrFI CCNGG 3 cut(s) 190, 738, 1411
BmsI GCATC 3 cut(s) 506, 1262, 1295
BpiI GAAGAC 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 3 cut(s) 151, 183, 826
BpuMI CCSGG 2 cut(s) 190, 738
BsaBI GATNNNNATC 1 cut(s) 1628
BsaI GGTCTC 3 cut(s) 22, 607, 1064
BsaJI CCNNGG 4 cut(s) 319, 933, 1236, 1410
BsaWI WCCGGW 1 cut(s) 1066
Bsc4I CCNNNNNNNGG 3 cut(s) 796, 1588, 1656
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 2 cut(s) 261, 1074
Bse3DI GCAATG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 1628
BseAI TCCGGA 1 cut(s) 1066
BseBI CCWGG 1 cut(s) 1411
BseDI CCNNGG 4 cut(s) 319, 933, 1236, 1410
BseGI GGATG 1 cut(s) 318
BseJI GATNNNNATC 1 cut(s) 1628
BseLI CCNNNNNNNGG 3 cut(s) 796, 1588, 1656
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 2 cut(s) 33, 218
BseNI ACTGG 2 cut(s) 261, 1074
BseRI GAGGAG 5 cut(s) 85, 88, 91, 208, 457
BseXI GCAGC 8 cut(s) 157, 168, 424, 673, 883, 993, 996, 1567
BsgI GTGCAG 1 cut(s) 1275
Bsh1236I CGCG 2 cut(s) 300, 825
BshFI GGCC 4 cut(s) 1176, 1209, 1337, 1592
BshNI GGYRCC 2 cut(s) 502, 1232
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 5 cut(s) 44, 190, 393, 738, 1067
BslFI GGGAC 2 cut(s) 412, 951
BslI CCNNNNNNNGG 3 cut(s) 796, 1588, 1656
BsmAI GTCTC 8 cut(s) 22, 607, 1063, 1064, 1082, 1341, 1444, 1459
BsmFI GGGAC 2 cut(s) 412, 951
BsmI GAATGC 2 cut(s) 1432, 1480
BsnI GGCC 4 cut(s) 1176, 1209, 1337, 1592
Bso31I GGTCTC 3 cut(s) 22, 607, 1064
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 95
Bsp13I TCCGGA 1 cut(s) 1066
Bsp143I GATC 4 cut(s) 208, 726, 763, 1570
Bsp19I CCATGG 2 cut(s) 933, 1236
BspACI CCGC 9 cut(s) 64, 73, 223, 300, 578, 653, 790, 823, 1177
BspANI GGCC 4 cut(s) 1176, 1209, 1337, 1592
BspCNI CTCAG 2 cut(s) 32, 217
BspEI TCCGGA 1 cut(s) 1066
BspFNI CGCG 2 cut(s) 300, 825
BspLI GGNNCC 6 cut(s) 504, 703, 960, 1234, 1416, 1593
BspMAI CTGCAG 2 cut(s) 414, 568
BspPI GGATC 1 cut(s) 216
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 2 cut(s) 502, 1232
BspTNI GGTCTC 3 cut(s) 22, 607, 1064
BsrBI CCGCTC 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 1 cut(s) 43
BsrI ACTGG 2 cut(s) 261, 1074
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 4 cut(s) 319, 933, 1236, 1410
BssMI GATC 4 cut(s) 208, 726, 763, 1570
BssSI CACGAG 1 cut(s) 1530
BssT1I CCWWGG 3 cut(s) 319, 933, 1236
Bst2BI CACGAG 1 cut(s) 1530
Bst2UI CCWGG 1 cut(s) 1411
Bst4CI ACNGT 3 cut(s) 56, 626, 1043
BstAPI GCANNNNNTGC 1 cut(s) 1259
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 7 cut(s) 45, 360, 657, 951, 1116, 1146, 1150
BstDEI CTNAG 5 cut(s) 19, 204, 308, 695, 1332
BstDSI CCRYGG 2 cut(s) 933, 1236
BstF5I GGATG 1 cut(s) 318
BstFNI CGCG 2 cut(s) 300, 825
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 3 cut(s) 305, 459, 827
BstKTI GATC 4 cut(s) 211, 729, 766, 1573
BstMAI GTCTC 8 cut(s) 22, 607, 1063, 1064, 1082, 1341, 1444, 1459
BstMBI GATC 4 cut(s) 208, 726, 763, 1570
BstMWI GCNNNNNNNGC 4 cut(s) 72, 671, 1259, 1442
BstNI CCWGG 1 cut(s) 1411
BstNSI RCATGY 1 cut(s) 1148
BstSCI CCNGG 3 cut(s) 188, 736, 1409
BstSFI CTRYAG 4 cut(s) 410, 564, 1311, 1654
BstUI CGCG 2 cut(s) 300, 825
BstV1I GCAGC 8 cut(s) 157, 168, 424, 673, 883, 993, 996, 1567
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 3 cut(s) 208, 726, 763
BstYI RGATCY 3 cut(s) 208, 726, 763
BsuI GTATCC 1 cut(s) 1378
BsuRI GGCC 4 cut(s) 1176, 1209, 1337, 1592
BtgI CCRYGG 2 cut(s) 933, 1236
BtgZI GCGATG 1 cut(s) 1615
BtsCI GGATG 1 cut(s) 318
BtsIMutI CAGTG 2 cut(s) 631, 1292
Cac8I GCNNGC 7 cut(s) 45, 360, 657, 951, 1116, 1146, 1150
CfoI GCGC 3 cut(s) 305, 459, 827
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 3 cut(s) 293, 316, 1591
Csp6I GTAC 3 cut(s) 193, 569, 1576
CviAII CATG 7 cut(s) 460, 683, 865, 934, 1145, 1227, 1237
CviQI GTAC 3 cut(s) 193, 569, 1576
DdeI CTNAG 5 cut(s) 19, 204, 308, 695, 1332
DpnI GATC 4 cut(s) 210, 728, 765, 1572
DpnII GATC 4 cut(s) 208, 726, 763, 1570
EaeI YGGCCR 2 cut(s) 1174, 1207
Ecl136II GAGCTC 1 cut(s) 93
Eco130I CCWWGG 3 cut(s) 319, 933, 1236
Eco147I AGGCCT 1 cut(s) 1337
Eco24I GRGCYC 1 cut(s) 95
Eco31I GGTCTC 3 cut(s) 22, 607, 1064
Eco47I GGWCC 2 cut(s) 293, 316
Eco53kI GAGCTC 1 cut(s) 93
Eco57I CTGAAG 4 cut(s) 645, 1287, 1410, 1571
EcoICRI GAGCTC 1 cut(s) 93
EcoO109I RGGNCCY 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 1 cut(s) 1409
EcoT14I CCWWGG 3 cut(s) 319, 933, 1236
EcoT38I GRGCYC 1 cut(s) 95
ErhI CCWWGG 3 cut(s) 319, 933, 1236
FaeI CATG 7 cut(s) 463, 686, 868, 937, 1148, 1230, 1240
FalI AAGNNNNNCTT 2 cut(s) 811, 843
FaqI GGGAC 2 cut(s) 412, 951
FatI CATG 7 cut(s) 459, 682, 864, 933, 1144, 1226, 1236
FauI CCCGC 2 cut(s) 230, 660
FauNDI CATATG 1 cut(s) 1513
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 1 cut(s) 95
FspBI CTAG 1 cut(s) 1451
GlaI GCGC 3 cut(s) 304, 458, 826
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 4 cut(s) 1176, 1209, 1337, 1592
HapII CCGG 5 cut(s) 44, 190, 393, 738, 1067
HhaI GCGC 3 cut(s) 305, 459, 827
Hin1II CATG 7 cut(s) 463, 686, 868, 937, 1148, 1230, 1240
Hin6I GCGC 3 cut(s) 303, 457, 825
HinP1I GCGC 3 cut(s) 303, 457, 825
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 1 cut(s) 1126
HinfI GANTC 9 cut(s) 85, 583, 808, 860, 908, 939, 1200, 1277, 1508
HpaII CCGG 5 cut(s) 44, 190, 393, 738, 1067
HphI GGTGA 4 cut(s) 44, 53, 62, 965
Hpy166II GTNNAC 2 cut(s) 643, 1578
Hpy188I TCNGA 4 cut(s) 81, 90, 543, 1670
Hpy188III TCNNGA 9 cut(s) 206, 230, 730, 805, 812, 1067, 1190, 1487, 1549
Hpy8I GTNNAC 2 cut(s) 643, 1578
Hpy99I CGWCG 2 cut(s) 109, 368
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 3 cut(s) 56, 626, 1043
HpyCH4IV ACGT 2 cut(s) 366, 474
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 671, 1259, 1442
HpyF3I CTNAG 5 cut(s) 19, 204, 308, 695, 1332
HpySE526I ACGT 2 cut(s) 366, 474
Hsp92II CATG 7 cut(s) 463, 686, 868, 937, 1148, 1230, 1240
HspAI GCGC 3 cut(s) 303, 457, 825
Kpn2I TCCGGA 1 cut(s) 1066
KroI GCCGGC 1 cut(s) 43
KroNI GCCGGC 1 cut(s) 45
Kzo9I GATC 4 cut(s) 208, 726, 763, 1570
LmnI GCTCC 4 cut(s) 98, 707, 964, 1614
Lsp1109I GCAGC 8 cut(s) 157, 168, 424, 673, 883, 993, 996, 1567
LweI GCATC 3 cut(s) 506, 1262, 1295
MaeI CTAG 1 cut(s) 1451
MaeII ACGT 2 cut(s) 366, 474
MalI GATC 4 cut(s) 210, 728, 765, 1572
MbiI CCGCTC 1 cut(s) 655
MboI GATC 4 cut(s) 208, 726, 763, 1570
MboII GAAGA 7 cut(s) 254, 545, 722, 758, 884, 1280, 1656
MfeI CAATTG 2 cut(s) 618, 1257
MflI RGATCY 3 cut(s) 208, 726, 763
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 9 cut(s) 151, 536, 605, 618, 769, 800, 1257, 1523, 1663
MlyI GAGTC 4 cut(s) 817, 869, 917, 933
MroI TCCGGA 1 cut(s) 1066
MroNI GCCGGC 1 cut(s) 43
MroXI GAANNNNTTC 3 cut(s) 537, 1245, 1664
MseI TTAA 6 cut(s) 131, 150, 477, 998, 1037, 1092
MslI CAYNNNNRTG 1 cut(s) 1137
MspI CCGG 5 cut(s) 44, 190, 393, 738, 1067
MspR9I CCNGG 3 cut(s) 190, 738, 1411
MunI CAATTG 2 cut(s) 618, 1257
Mva1269I GAATGC 2 cut(s) 1432, 1480
MvaI CCWGG 1 cut(s) 1411
MvnI CGCG 2 cut(s) 300, 825
MwoI GCNNNNNNNGC 4 cut(s) 72, 671, 1259, 1442
NaeI GCCGGC 1 cut(s) 45
NciI CCSGG 2 cut(s) 190, 738
NcoI CCATGG 2 cut(s) 933, 1236
NdeI CATATG 1 cut(s) 1513
NdeII GATC 4 cut(s) 208, 726, 763, 1570
NgoMIV GCCGGC 1 cut(s) 43
NlaIII CATG 7 cut(s) 463, 686, 868, 937, 1148, 1230, 1240
NlaIV GGNNCC 6 cut(s) 504, 703, 960, 1234, 1416, 1593
NspI RCATGY 1 cut(s) 1148
NspV TTCGAA 1 cut(s) 240
PaeI GCATGC 1 cut(s) 1148
PceI AGGCCT 1 cut(s) 1337
PcsI WCGNNNNNNNCGW 1 cut(s) 1579
PctI GAATGC 2 cut(s) 1432, 1480
PdiI GCCGGC 1 cut(s) 45
PdmI GAANNNNTTC 3 cut(s) 537, 1245, 1664
PfeI GAWTC 5 cut(s) 85, 583, 1200, 1277, 1508
PleI GAGTC 4 cut(s) 816, 868, 916, 933
PpsI GAGTC 4 cut(s) 816, 868, 916, 933
PpuMI RGGWCCY 1 cut(s) 293
PsiI TTATAA 1 cut(s) 722
Psp124BI GAGCTC 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 1409
PspGI CCWGG 1 cut(s) 1409
PspN4I GGNNCC 6 cut(s) 504, 703, 960, 1234, 1416, 1593
PspPI GGNCC 3 cut(s) 293, 316, 1591
PspPPI RGGWCCY 1 cut(s) 293
PsrI GAACNNNNNNTAC 2 cut(s) 109, 141
PstI CTGCAG 2 cut(s) 414, 568
PsuI RGATCY 3 cut(s) 208, 726, 763
RsaI GTAC 3 cut(s) 194, 570, 1577
RsaNI GTAC 3 cut(s) 193, 569, 1576
RseI CAYNNNNRTG 1 cut(s) 1137
SacI GAGCTC 1 cut(s) 95
SaqAI TTAA 6 cut(s) 131, 150, 477, 998, 1037, 1092
Sau3AI GATC 4 cut(s) 208, 726, 763, 1570
Sau96I GGNCC 3 cut(s) 293, 316, 1591
SchI GAGTC 4 cut(s) 817, 869, 917, 933
ScrFI CCNGG 3 cut(s) 190, 738, 1411
SduI GDGCHC 1 cut(s) 95
SfaNI GCATC 3 cut(s) 506, 1262, 1295
SfcI CTRYAG 4 cut(s) 410, 564, 1311, 1654
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 2 cut(s) 293, 316
SmiMI CAYNNNNRTG 1 cut(s) 1137
SmlI CTYRAG 3 cut(s) 166, 198, 805
SmoI CTYRAG 3 cut(s) 166, 198, 805
SphI GCATGC 1 cut(s) 1148
Sse9I AATT 9 cut(s) 151, 536, 605, 618, 769, 800, 1257, 1523, 1663
SseBI AGGCCT 1 cut(s) 1337
SsiI CCGC 9 cut(s) 64, 73, 223, 300, 578, 653, 790, 823, 1177
SspMI CTAG 1 cut(s) 1451
SstI GAGCTC 1 cut(s) 95
StuI AGGCCT 1 cut(s) 1337
StyD4I CCNGG 3 cut(s) 188, 736, 1409
StyI CCWWGG 3 cut(s) 319, 933, 1236
TaaI ACNGT 3 cut(s) 56, 626, 1043
TaiI ACGT 2 cut(s) 369, 477
TaqI TCGA 6 cut(s) 11, 107, 140, 240, 1488, 1642
TasI AATT 9 cut(s) 151, 536, 605, 618, 769, 800, 1257, 1523, 1663
TauI GCSGC 3 cut(s) 303, 825, 1179
TfiI GAWTC 5 cut(s) 85, 583, 1200, 1277, 1508
Tru1I TTAA 6 cut(s) 131, 150, 477, 998, 1037, 1092
Tru9I TTAA 6 cut(s) 131, 150, 477, 998, 1037, 1092
TscAI CASTG 2 cut(s) 631, 1299
TseI GCWGC 8 cut(s) 145, 156, 412, 686, 896, 981, 984, 1555
TspDTI ATGAA 6 cut(s) 481, 648, 671, 819, 1290, 1487
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 2 cut(s) 631, 1299
VpaK11BI GGWCC 2 cut(s) 293, 316
XapI RAATTY 4 cut(s) 536, 769, 800, 1523
XceI RCATGY 1 cut(s) 1148
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XmnI GAANNNNTTC 3 cut(s) 537, 1245, 1664
XspI CTAG 1 cut(s) 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.