Rorug01G0373000

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006647
Physical Location & Seq
Forward (+)
48627618 .. 48630434
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0373000.1

Sequence Viewer

Length: 2817 bp
ATGGCTGAGGCACTTGTTAAAGTTCTCCTTGAGCGTTTGGCTACGGTCGCCCTTGGCAAGGTTCGAGGAGAGTTCAAACTGGTCTTTGGGTTTGAGGAAGAAGTCAAGAGCCTCGCCCGCAATCTAGAAGCTATTCAAGCTGTGCTGGAGGATGCAGAGCAGAGGCAAGTGACGGAGGCAACCGTGAGACGTTGGCTGAGTGAACTGACTGAAGTTTCCTACGAGATAGATGACGTTTTGGATGAGTGGATCACTCAAGTTCGAAAGAGACAGATGGAGAAAGAAGAAAAGCATGCTCTTGCTATTGCTAAGAAGGTACGTTCCTCTGCCTCCTCTTTCATTTCTGGTAGCAAGGTCAGGCGGTTTATTCATCGTCGCGAAATGGCTGGGAGGATTAAAGAGCTAAATGAAAGATTAGCTTTGATTGATAAGGAAAAACAAAGTTTTAATTTTCAAAACACCACAAGAGACCCTCAACTACTTGTGCGGCAAAAAACTACTTCTCTTCCCAATGTTAAAACATTTGGTCGAGATAATGAAAAAAACCTCATAGTTAGCAAGCTGCTAAGTGAGAGTAGTGAAGGAAATGAGGCTCCTCTTATCATTCCTATTGTAGGGATGGGAGGAATGGGGAAAACAACTCTTGCTCAACTTGTTTACAATGATGAAAATGTGAAATCTTATTTTGACAAGAGAATATGGGTTTGTGTCTCGGACCCGTTTGAAGAGATCAAGATTGCTCAAGCTATCATTGAATGTCTAGATAGAGATAATACCTCAAAAAATTCAAATCAGTTGCAAATTCTTGTGGAATGCATATATGAATTGCTTGAGGGTAAAAAGTTTCTCCTTGTCTTAGACGATGTGTGGAGCCCAAAACTTACTCAATGGGAAGAATTGATTAAATCTTTATGTAATGGTGCTATTGGCAGTAGAGTATTGGTGACCACTCGAAAGAAGGAGGTTGCTACTTTAATGAAAGCAACTACTCAATTGATCCTTTTGGAGGAGTTGGCTCAAACATTTTGTTTGTCATTGTTCTATTACAATGCAGACATGAATGAGAGTAGTGTGTCTCAAGAGTTACAAGATATTGGGTTAGAGATTGTGAAAAGGTGCAATGGGTTGCCTCTTGCTGCAAAGACATTAGGAAGCCTTATGCGCAATAAGAAAAAGATTCATGAATGGCAAGCTATTTTAAGAAGTAAGACATGGGAGTTGAAAGAGATTCAACAAGATGTTTTTCGACCACTATTACTAAGTTATCATGATTTGACTCCCACAATCAAACGTTGTCTTTTGTACTGTGTTATATTTCCCAAAAATTATGTATATAATAAAAATAATTTGATTGAGTTGTGGATGTCACAAGATTATCTGGATGCCAAAGGAAGTAAAGAAAAGATAACTATTGGTCAAAATTATTTTGATGACTTAGGAATGCGGTCATTCTTTCAAGATGTTGTGGTAGATGCTAAGATTGAAAACATAACATGCAAAATTCATGATATTGTACATGACTTTCTACAATATTTGACCAAGAATGAATGTTTGATTCAAGATTTTGAGTTGGGTGCTAGTGAAAGAACGGAGGTGCCAAATGATAAAGTTCATCATTTGACCTTATTGCATGCAACCGAGAGTGATTCTTTGGTATCTTTTCTCCTTAGTTATAAAAAGTTACGTACCTTGACAGCTATAGATTCATCCAATAATTCCACTAACCCAATATTTGAGTTGACAGTACAATTGAAATGCCTTAGGACAGTAATCTTGCATCCTAGGAACAATGGTCTCGTAGAAATTCCCGAAATGATAGGTGAATTGATACATTTGAGGTATCTTGATTTATCTGGTAATGACAAGTTAAAAGAATTGCCCAGCGCCATGGGTAGCTTATACAACTTGCAAACCTTGCGACTTGTTGGTTGTGACAGTCTCCGAAAAGTAGATGTGCGAAGGCTGATTAACTTGAGGCATCTTTATGTTGATGGCTGTGGTTCCCTAAGCATAATACAAGGAATTGAGAAATTAACAGATCTGCAAAGGCTAGATAGGTTTCGTGTCGGAGATTGTGATGAAGAAAACAAGTTCGAAGATCTGAAAGACTTAAACCAGCTTCAAGGGGGTCTTACTATTGAGACTAAGGGAAATCCAAATATGGCGGAGAAAGCAATTATGGTGAATAAGGCTCACCTCCTGCAATTGAAACTAGACTTCCAATTGAGTTATTATGCAGAGCGGCAAAGAGAAATTATGAATGGCTTAGAGCTGCATCCATGTTTGGAATCTTTATCGATCTCTTATTATGAAGGCGGCGCCTTCTCCCGTTGGTTGTTGTCTTTACACAGCTTGAGAGTCCTCACCCTCAAGGAGTGCAACAATTGTCGGTCTTTTCCTCCTTTAGGGAAACTGGCGTCTCTTGAATCATTGCATATTGAGAGACTCGATGGAGTTGAGAAGGTAGGAGTTAAGTTTTTGGGAATAGATGGGCAAATCTCGTCCTCCATTTTCATATCATTCCCGAAGCTGGAAAAACTCACCATCGAAGACATGCGGTTATGGAAAGTGTGGGAAGGAGTGGAGGGTTGGATGCGGGAAGAGGAGAATTCTGAGAATAATAATATTACAATTATGCCATGCCTCTCTCAGTTGACAATTTCTTTGTGCTGGAACATAAAAGCACTGCCAGACTTCCTGTGGAAGACACCACTACAGAAATTGCACATCAAGGCATCTCCATTTCTCCAAGAACTTTACAGAAGAAAAAAAAAAAGGGCCGAGATTTCTCACATCCCAAACATCAAATTAGGATGA

Protein Analysis

938

Amino Acids

107.66

Weight (kDa)

7.01

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 96 4.2e-23 Rx N-terminal domain
NB-ARC PF00931 184 - 354 6.1e-36 NB-ARC domain
WHD_DRP PF23559 438 - 508 2.6e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 561 - 856 1.8e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 699 - 815 7.6e-25 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1674
Acc16I TGCGCA 1 cut(s) 1163
AccB1I GGYRCC 2 cut(s) 1594, 2318
AccBSI CCGCTC 1 cut(s) 2242
AccII CGCG 1 cut(s) 378
AciI CCGC 9 cut(s) 118, 361, 487, 1444, 2165, 2242, 2316, 2557, 2596
AclI AACGTT 1 cut(s) 1291
AclWI GGATC 2 cut(s) 257, 991
AcsI RAATTY 5 cut(s) 784, 801, 1500, 1803, 2608
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 2 cut(s) 2319, 2417
AfaI GTAC 5 cut(s) 318, 1304, 1515, 1687, 1746
AfiI CCNNNNNNNGG 5 cut(s) 58, 614, 1006, 2405, 2530
AlwI GGATC 2 cut(s) 257, 991
AoxI GGCC 1 cut(s) 2778
ApeKI GCWGC 3 cut(s) 562, 1136, 2272
ApoI RAATTY 5 cut(s) 784, 801, 1500, 1803, 2608
ArsI GACNNNNNNTTYG 2 cut(s) 2647, 2679
Asp700I GAANNNNTTC 1 cut(s) 132
AspA2I CCTAGG 1 cut(s) 1781
AspLEI GCGC 3 cut(s) 1164, 1886, 2321
AspS9I GGNCC 2 cut(s) 715, 2778
AsuHPI GGTGA 6 cut(s) 955, 1832, 2186, 2194, 2356, 2533
AsuII TTCGAA 2 cut(s) 262, 2094
AvaII GGWCC 1 cut(s) 715
AvrII CCTAGG 1 cut(s) 1781
AxyI CCTNAGG 1 cut(s) 1760
BanI GGYRCC 2 cut(s) 1594, 2318
BanII GRGCYC 1 cut(s) 875
BbsI GAAGAC 2 cut(s) 2556, 2711
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 3 cut(s) 549, 1123, 2259
BccI CCATC 6 cut(s) 268, 613, 1985, 2444, 2483, 2552
BfaI CTAG 6 cut(s) 125, 761, 1578, 1782, 2051, 2213
BfmI CTRYAG 2 cut(s) 1698, 2714
BfoI RGCGCY 2 cut(s) 1887, 2322
BglII AGATCT 2 cut(s) 2038, 2098
BisI GCNGC 6 cut(s) 488, 563, 1137, 2243, 2273, 2317
BlnI CCTAGG 1 cut(s) 1781
BlsI GCNGC 6 cut(s) 489, 564, 1138, 2244, 2274, 2318
Bme18I GGWCC 1 cut(s) 715
BmgT120I GGNCC 2 cut(s) 715, 2778
BmiI GGNNCC 6 cut(s) 594, 717, 872, 1596, 2002, 2320
BmsI GCATC 8 cut(s) 142, 1372, 1462, 1786, 1987, 2284, 2583, 2744
BpiI GAAGAC 2 cut(s) 2556, 2711
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 2 cut(s) 6, 2006
Bpu14I TTCGAA 2 cut(s) 262, 2094
BpuEI CTTGAG 8 cut(s) 50, 240, 726, 851, 1062, 1993, 2354, 2374
Bsa29I ATCGAT 1 cut(s) 2297
BsaAI YACGTR 1 cut(s) 1685
BsaHI GRCGYC 2 cut(s) 2319, 2417
BsaI GGTCTC 2 cut(s) 462, 1799
BsaJI CCNNGG 3 cut(s) 52, 1781, 1887
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 614, 1006, 2405, 2530
Bse1I ACTGG 2 cut(s) 84, 2418
Bse21I CCTNAGG 1 cut(s) 1760
Bse3DI GCAATG 2 cut(s) 1126, 2429
BseCI ATCGAT 1 cut(s) 2297
BseDI CCNNGG 3 cut(s) 52, 1781, 1887
BseLI CCNNNNNNNGG 5 cut(s) 58, 614, 1006, 2405, 2530
BseMI GCAATG 2 cut(s) 1126, 2429
BseMII CTCAG 3 cut(s) 188, 2604, 2663
BseNI ACTGG 2 cut(s) 84, 2418
BseRI GAGGAG 5 cut(s) 81, 322, 585, 1022, 2618
BseXI GCAGC 3 cut(s) 549, 1123, 2259
BseYI CCCAGC 2 cut(s) 386, 1880
Bsh1236I CGCG 1 cut(s) 378
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 2780
BshNI GGYRCC 2 cut(s) 1594, 2318
BshVI ATCGAT 1 cut(s) 2297
BsiEI CGRYCG 1 cut(s) 48
BslI CCNNNNNNNGG 5 cut(s) 58, 614, 1006, 2405, 2530
BsmBI CGTCTC 2 cut(s) 181, 2424
BsmI GAATGC 2 cut(s) 818, 1446
BsnI GGCC 1 cut(s) 2780
Bso31I GGTCTC 2 cut(s) 462, 1799
Bsp119I TTCGAA 2 cut(s) 262, 2094
Bsp1286I GDGCHC 1 cut(s) 875
Bsp1407I TGTACA 1 cut(s) 1513
Bsp143I GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
Bsp19I CCATGG 1 cut(s) 1887
Bsp68I TCGCGA 1 cut(s) 378
BspACI CCGC 9 cut(s) 118, 361, 487, 1444, 2165, 2242, 2316, 2557, 2596
BspANI GGCC 1 cut(s) 2780
BspCNI CTCAG 3 cut(s) 189, 2605, 2662
BspDI ATCGAT 1 cut(s) 2297
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 3 cut(s) 1180, 1267, 1504
BspLI GGNNCC 6 cut(s) 594, 717, 872, 1596, 2002, 2320
BspPI GGATC 2 cut(s) 257, 991
BspT104I TTCGAA 2 cut(s) 262, 2094
BspT107I GGYRCC 2 cut(s) 1594, 2318
BspTNI GGTCTC 2 cut(s) 462, 1799
BsrBI CCGCTC 1 cut(s) 2242
BsrDI GCAATG 2 cut(s) 1126, 2429
BsrGI TGTACA 1 cut(s) 1513
BsrI ACTGG 2 cut(s) 84, 2418
BssECI CCNNGG 3 cut(s) 52, 1781, 1887
BssMI GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
BssNI GRCGYC 2 cut(s) 2319, 2417
BssT1I CCWWGG 3 cut(s) 52, 1781, 1887
Bst4CI ACNGT 6 cut(s) 46, 184, 1307, 1744, 1768, 1937
Bst6I CTCTTC 3 cut(s) 510, 720, 2595
BstACI GRCGYC 2 cut(s) 2319, 2417
BstAPI GCANNNNNTGC 1 cut(s) 1915
BstAUI TGTACA 1 cut(s) 1513
BstBAI YACGTR 1 cut(s) 1685
BstBI TTCGAA 2 cut(s) 262, 2094
BstC8I GCNNGC 5 cut(s) 118, 294, 560, 1191, 1632
BstDSI CCRYGG 1 cut(s) 1887
BstEII GGTNACC 1 cut(s) 943
BstENI CCTNNNNNAGG 4 cut(s) 56, 612, 1004, 2403
BstFNI CGCG 1 cut(s) 378
BstH2I RGCGCY 2 cut(s) 1887, 2322
BstHHI GCGC 3 cut(s) 1164, 1886, 2321
BstKTI GATC 6 cut(s) 252, 732, 999, 2041, 2101, 2301
BstMBI GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 6 cut(s) 47, 117, 137, 1161, 1915, 2171
BstNSI RCATGY 4 cut(s) 296, 1497, 1634, 2557
BstPI GGTNACC 1 cut(s) 943
BstSFI CTRYAG 2 cut(s) 1698, 2714
BstSNI TACGTA 1 cut(s) 1685
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 3 cut(s) 549, 1123, 2259
BstV2I GAAGAC 2 cut(s) 2556, 2711
BstX2I RGATCY 2 cut(s) 2038, 2098
BstXI CCANNNNNNTGG 1 cut(s) 1888
BstYI RGATCY 2 cut(s) 2038, 2098
Bsu15I ATCGAT 1 cut(s) 2297
Bsu36I CCTNAGG 1 cut(s) 1760
BsuRI GGCC 1 cut(s) 2780
BsuTUI ATCGAT 1 cut(s) 2297
BtgI CCRYGG 1 cut(s) 1887
BtsI GCAGTG 1 cut(s) 2684
BtsIMutI CAGTG 1 cut(s) 2684
BtuMI TCGCGA 1 cut(s) 378
Cac8I GCNNGC 5 cut(s) 118, 294, 560, 1191, 1632
CciI TCATGA 3 cut(s) 1180, 1267, 1504
CfoI GCGC 3 cut(s) 1164, 1886, 2321
Cfr13I GGNCC 2 cut(s) 715, 2778
ClaI ATCGAT 1 cut(s) 2297
CseI GACGC 1 cut(s) 2406
Csp6I GTAC 5 cut(s) 317, 1303, 1514, 1686, 1745
CviQI GTAC 5 cut(s) 317, 1303, 1514, 1686, 1745
DinI GGCGCC 1 cut(s) 2320
DpnI GATC 6 cut(s) 251, 731, 998, 2040, 2100, 2300
DpnII GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
Eam1104I CTCTTC 3 cut(s) 510, 720, 2595
EarI CTCTTC 3 cut(s) 510, 720, 2595
EciI GGCGGA 1 cut(s) 2180
Eco105I TACGTA 1 cut(s) 1685
Eco130I CCWWGG 3 cut(s) 52, 1781, 1887
Eco24I GRGCYC 1 cut(s) 875
Eco31I GGTCTC 2 cut(s) 462, 1799
Eco47I GGWCC 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 231
Eco81I CCTNAGG 1 cut(s) 1760
Eco91I GGTNACC 1 cut(s) 943
EcoNI CCTNNNNNAGG 4 cut(s) 56, 612, 1004, 2403
EcoO65I GGTNACC 1 cut(s) 943
EcoRI GAATTC 1 cut(s) 2608
EcoT14I CCWWGG 3 cut(s) 52, 1781, 1887
EcoT22I ATGCAT 1 cut(s) 818
EcoT38I GRGCYC 1 cut(s) 875
EgeI GGCGCC 1 cut(s) 2320
EheI GGCGCC 1 cut(s) 2320
ErhI CCWWGG 3 cut(s) 52, 1781, 1887
Esp3I CGTCTC 2 cut(s) 181, 2424
FalI AAGNNNNNCTT 6 cut(s) 12, 44, 403, 435, 2115, 2147
FauI CCCGC 2 cut(s) 125, 2589
Fnu4HI GCNGC 6 cut(s) 488, 563, 1137, 2243, 2273, 2317
FriOI GRGCYC 1 cut(s) 875
Fsp4HI GCNGC 6 cut(s) 488, 563, 1137, 2243, 2273, 2317
FspBI CTAG 6 cut(s) 125, 761, 1578, 1782, 2051, 2213
FspI TGCGCA 1 cut(s) 1163
GlaI GCGC 3 cut(s) 1163, 1885, 2320
GluI GCNGC 6 cut(s) 488, 563, 1137, 2243, 2273, 2317
GsaI CCCAGC 2 cut(s) 390, 1884
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 2 cut(s) 1887, 2322
HaeIII GGCC 1 cut(s) 2780
HgaI GACGC 1 cut(s) 2406
HhaI GCGC 3 cut(s) 1164, 1886, 2321
Hin1I GRCGYC 2 cut(s) 2319, 2417
Hin6I GCGC 3 cut(s) 1162, 1884, 2319
HinP1I GCGC 3 cut(s) 1162, 1884, 2319
HincII GTYRAC 2 cut(s) 1740, 2655
HindII GTYRAC 2 cut(s) 1740, 2655
HphI GGTGA 6 cut(s) 955, 1832, 2186, 2194, 2356, 2533
Hpy166II GTNNAC 4 cut(s) 203, 658, 1740, 2655
Hpy188I TCNGA 5 cut(s) 715, 1943, 2069, 2103, 2614
Hpy8I GTNNAC 4 cut(s) 203, 658, 1740, 2655
Hpy99I CGWCG 1 cut(s) 378
HpyAV CCTTC 8 cut(s) 307, 575, 952, 1953, 2306, 2332, 2455, 2570
HpyCH4III ACNGT 6 cut(s) 46, 184, 1307, 1744, 1768, 1937
HpyCH4IV ACGT 5 cut(s) 190, 234, 319, 1291, 1684
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 117, 137, 1161, 1915, 2171
HpySE526I ACGT 5 cut(s) 190, 234, 319, 1291, 1684
Hsp92I GRCGYC 2 cut(s) 2319, 2417
HspAI GCGC 3 cut(s) 1162, 1884, 2319
KasI GGCGCC 1 cut(s) 2318
Kzo9I GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
LmnI GCTCC 2 cut(s) 598, 870
Lsp1109I GCAGC 3 cut(s) 549, 1123, 2259
LweI GCATC 8 cut(s) 142, 1372, 1462, 1786, 1987, 2284, 2583, 2744
MaeI CTAG 6 cut(s) 125, 761, 1578, 1782, 2051, 2213
MaeII ACGT 5 cut(s) 190, 234, 319, 1291, 1684
MaeIII GTNAC 6 cut(s) 169, 943, 1083, 1365, 1680, 1931
MalI GATC 6 cut(s) 251, 731, 998, 2040, 2100, 2300
MbiI CCGCTC 1 cut(s) 2242
MboI GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
MfeI CAATTG 5 cut(s) 992, 1748, 2204, 2222, 2383
MflI RGATCY 2 cut(s) 2038, 2098
MhlI GDGCHC 1 cut(s) 875
Mly113I GGCGCC 1 cut(s) 2319
MlyI GAGTC 3 cut(s) 1270, 2367, 2439
MmeI TCCRAC 2 cut(s) 2047, 2570
Mph1103I ATGCAT 1 cut(s) 818
MroXI GAANNNNTTC 1 cut(s) 132
MslI CAYNNNNRTG 1 cut(s) 1983
MunI CAATTG 5 cut(s) 992, 1748, 2204, 2222, 2383
Mva1269I GAATGC 2 cut(s) 818, 1446
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 6 cut(s) 47, 117, 137, 1161, 1915, 2171
NarI GGCGCC 1 cut(s) 2319
NcoI CCATGG 1 cut(s) 1887
NdeII GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
NlaIV GGNNCC 6 cut(s) 594, 717, 872, 1596, 2002, 2320
NmeAIII GCCGAG 1 cut(s) 2806
NmuCI GTSAC 4 cut(s) 169, 943, 1365, 1931
NruI TCGCGA 1 cut(s) 378
NsbI TGCGCA 1 cut(s) 1163
NsiI ATGCAT 1 cut(s) 818
NspI RCATGY 4 cut(s) 296, 1497, 1634, 2557
NspV TTCGAA 2 cut(s) 262, 2094
PaeI GCATGC 2 cut(s) 296, 1634
PagI TCATGA 3 cut(s) 1180, 1267, 1504
PctI GAATGC 2 cut(s) 818, 1446
PdmI GAANNNNTTC 1 cut(s) 132
PfeI GAWTC 7 cut(s) 1177, 1228, 1555, 1646, 1703, 2288, 2426
PkrI GCNGC 6 cut(s) 489, 564, 1138, 2244, 2274, 2318
PleI GAGTC 3 cut(s) 1270, 2366, 2439
PluTI GGCGCC 1 cut(s) 2322
PpsI GAGTC 3 cut(s) 1270, 2366, 2439
Ppu21I YACGTR 1 cut(s) 1685
PsiI TTATAA 1 cut(s) 1674
Psp1406I AACGTT 1 cut(s) 1291
PspEI GGTNACC 1 cut(s) 943
PspFI CCCAGC 2 cut(s) 386, 1880
PspN4I GGNNCC 6 cut(s) 594, 717, 872, 1596, 2002, 2320
PspPI GGNCC 2 cut(s) 715, 2778
PsuI RGATCY 2 cut(s) 2038, 2098
RruI TCGCGA 1 cut(s) 378
RsaI GTAC 5 cut(s) 318, 1304, 1515, 1687, 1746
RsaNI GTAC 5 cut(s) 317, 1303, 1514, 1686, 1745
RseI CAYNNNNRTG 1 cut(s) 1983
SatI GCNGC 6 cut(s) 488, 563, 1137, 2243, 2273, 2317
Sau3AI GATC 6 cut(s) 249, 729, 996, 2038, 2098, 2298
Sau96I GGNCC 2 cut(s) 715, 2778
SchI GAGTC 3 cut(s) 1270, 2367, 2439
SduI GDGCHC 1 cut(s) 875
SfaNI GCATC 8 cut(s) 142, 1372, 1462, 1786, 1987, 2284, 2583, 2744
SfcI CTRYAG 2 cut(s) 1698, 2714
SfoI GGCGCC 1 cut(s) 2320
SfuI TTCGAA 2 cut(s) 262, 2094
SinI GGWCC 1 cut(s) 715
SmiMI CAYNNNNRTG 1 cut(s) 1983
SmlI CTYRAG 8 cut(s) 29, 255, 741, 830, 1077, 1972, 2353, 2369
SmoI CTYRAG 8 cut(s) 29, 255, 741, 830, 1077, 1972, 2353, 2369
SnaBI TACGTA 1 cut(s) 1685
SphI GCATGC 2 cut(s) 296, 1634
SsiI CCGC 9 cut(s) 118, 361, 487, 1444, 2165, 2242, 2316, 2557, 2596
SspDI GGCGCC 1 cut(s) 2318
SspI AATATT 3 cut(s) 1532, 1731, 2626
SspMI CTAG 6 cut(s) 125, 761, 1578, 1782, 2051, 2213
StyI CCWWGG 3 cut(s) 52, 1781, 1887
TaaI ACNGT 6 cut(s) 46, 184, 1307, 1744, 1768, 1937
TaiI ACGT 5 cut(s) 193, 237, 322, 1294, 1687
TaqI TCGA 9 cut(s) 64, 262, 529, 952, 1246, 2094, 2297, 2448, 2547
TaqII GACCGA 1 cut(s) 2379
TatI WGTACW 3 cut(s) 1302, 1513, 1744
TauI GCSGC 3 cut(s) 490, 2245, 2319
TfiI GAWTC 7 cut(s) 1177, 1228, 1555, 1646, 1703, 2288, 2426
TscAI CASTG 1 cut(s) 2691
TseFI GTSAC 4 cut(s) 169, 943, 1365, 1931
TseI GCWGC 3 cut(s) 562, 1136, 2272
Tsp45I GTSAC 4 cut(s) 169, 943, 1365, 1931
TspGWI ACGGA 2 cut(s) 188, 1604
TspRI CASTG 1 cut(s) 2691
VpaK11BI GGWCC 1 cut(s) 715
XagI CCTNNNNNAGG 4 cut(s) 56, 612, 1004, 2403
XapI RAATTY 5 cut(s) 784, 801, 1500, 1803, 2608
XbaI TCTAGA 2 cut(s) 124, 760
XceI RCATGY 4 cut(s) 296, 1497, 1634, 2557
XcmI CCANNNNNNNNNTGG 1 cut(s) 2697
XmaJI CCTAGG 1 cut(s) 1781
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 6 cut(s) 125, 761, 1578, 1782, 2051, 2213
Zsp2I ATGCAT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.