Rroxscaffold_4G00284830

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006647
Physical Location & Seq
Reverse (-)
6346917 .. 6364037
17121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284830.1

Sequence Viewer

Length: 1770 bp
ATGTACAACATAAACTTCCAAACAAATCCGCCGAGCCACGTGGCATCTCCTCTTTCACTTACATTCTGTACAACGATTCTCTCCTTCTTTGCTCCCAAGTACAGAGAGATGGCCTTGGTCTCGGCTGTGAGAGGCGGCCGGCTCCCCTCATCATTACATCATCATCATCATCATCTGGGTTCGTCTTCGTCTTCATCACTTGGTAGTTGGGGTTCGTTAAGACCGTGGAAGCAGATGAATTCTAGCTCAAAAAGCTCGAAGGGCTTCACTCTCTTGGCTCGTTACTCACAAACACAGGACCTTTTCTCCTCTCGCCTTCAAGATAGTATGGAAAACTTGCCTAAACTGGTGGAGGATATTGTCCGGACATCATTAAGTACAGGCCCACGTGGCGCCCTAAGGATGGCTCAAGGTATTCAAGCATTTATTGGTGTTGGTGGGGAGTGGCTTACTGAGGCATCAAAGCATATCAATTTCAAATTCATCTACTGGCTTACCAACCCAATTGCAGCTTGGAGTGCTCTCCCCCTTTACTTGAGGAGATTGTTTGAGCGCATGGGAGCAACTTATATTAAATTGGGCCAGTTTATTGCATCCACACCAACATTGTTCCCAGCAGACTATGTTGAGGAATTTCAGAACTGCTTTGACAGAGCTCCCGCAATACCTTTTGTGGAGATTCAACAAATATTGCGCCAGGAGTTGGGGAGACCAATTGAAAGTGTATTTGAGTATGTTGACCCAACTCCAATTGCTTCGGCCTCAATAGCACAAGTTCACGGTGCAAGGCTAAGAGGGTCCCAAGAAGATGTGGTTATAAAGGTCTTGAAACCTGGAATAGAAGATATGTTGGTTGCAGATCTGAATTTTATTTACATAGTTGCTCGCATTTTGGAGTTCTTGAGTCCTGACATAAGCCGTGCTTCACTGGTTGGTATTGTGAAGGACATACGGGAATCTATGCTTGAAGAGGTTGATTTCTACAAGGAGGCTGCAAATGTTGAGTCCTTCAGGAGATATTTAGAAGCCATGGGACTCACAAGGCAGGCTACAGCTCCAAGAGTGTATCACCAATGTAGCACAAAACGAATTTTAACAATGGAGAGGCTCTATGGGGTTCCTCTTACTGACTTAGACTCTATAAGTTCACTTGTTTCTAGTCCGGAGGCCAGTCTCATAACTGCCCTTAATGTGTGGTTTGGCAGTTTACTTGCCTGTGAAAGCTTCCATGCAGATGTGCACGCTGGAAATCTGTGGTTGTTGCGTGATGGCCGTATTGGGTTTCTTGACTTTGGAATCGTAGGTCGTATAGACCCACAAACATGGGCTGCCATGGAAATCTTTTTGGCATCAATTGCAACTGAAGAATATGAATCAATGGCATCTGCGTTGATTCAAATGGGTGCTACAGACACAAATGTTGATGCTAAGGCCTTTGCAAGAGACTTGGAAAAAATTCCAGGAAAAATCATAAAAGATGGAAACTCTTGTATTTTGATCGGTACACCCATATTGTACCCAAGCAAAGCCGCTTCTGTGTTGATGAAGTGTTCTCTTATGGAAAAGGTATTCCCTGACTTGATTATGATTTCAGAATCAACTGGTAGCCGATTCAAAAGAGAGAGGCAGTTACTGACATTTCAACTCAATCTATTTAATGGCTACTCTCAGCAATGCACACCAGGCATGTCTTGTCTAGAAATTAAAAGAGAAACAAAAACTGAGTCCAAGGAGACAGTAAGTCATCAATATCATTTGAGAAATTTTTGA

Protein Analysis

589

Amino Acids

65.89

Weight (kDa)

7.23

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 216 - 462 2.7e-59 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 703
AccIII TCCGGA 2 cut(s) 363, 1162
AciI CCGC 4 cut(s) 29, 135, 660, 1530
AcoI YGGCCR 2 cut(s) 136, 1270
AcsI RAATTY 7 cut(s) 238, 479, 632, 865, 1089, 1455, 1762
AcuI CTGAAG 2 cut(s) 994, 1383
AcvI CACGTG 2 cut(s) 40, 389
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 6 cut(s) 5, 70, 101, 379, 1504, 1517
AfiI CCNNNNNNNGG 2 cut(s) 403, 703
AhdI GACNNNNNGTC 1 cut(s) 1740
AjnI CCWGG 4 cut(s) 696, 832, 1459, 1681
AluBI AGCT 6 cut(s) 246, 255, 512, 656, 1055, 1224
AluI AGCT 6 cut(s) 246, 255, 512, 656, 1055, 1224
Alw21I GWGCWC 3 cut(s) 523, 658, 1242
Alw26I GTCTC 5 cut(s) 124, 703, 1178, 1437, 1727
Alw44I GTGCAC 1 cut(s) 1238
AlwNI CAGNNNCTG 1 cut(s) 1633
Aor13HI TCCGGA 2 cut(s) 363, 1162
AoxI GGCC 8 cut(s) 111, 136, 382, 580, 759, 1167, 1270, 1431
ApaLI GTGCAC 1 cut(s) 1238
ApeKI GCWGC 3 cut(s) 509, 992, 1328
ApoI RAATTY 7 cut(s) 238, 479, 632, 865, 1089, 1455, 1762
ArsI GACNNNNNNTTYG 4 cut(s) 989, 1021, 1709, 1741
Asp700I GAANNNNTTC 2 cut(s) 263, 1455
AspLEI GCGC 3 cut(s) 395, 555, 696
AspS9I GGNCC 4 cut(s) 298, 383, 580, 798
AsuHPI GGTGA 1 cut(s) 1061
AvaII GGWCC 2 cut(s) 298, 798
AxyI CCTNAGG 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 1242
BanI GGYRCC 1 cut(s) 392
BanII GRGCYC 1 cut(s) 658
BbrPI CACGTG 2 cut(s) 40, 389
BbsI GAAGAC 2 cut(s) 177, 183
Bbv12I GWGCWC 3 cut(s) 523, 658, 1242
BbvI GCAGC 3 cut(s) 521, 979, 1315
BccI CCATC 4 cut(s) 103, 397, 1262, 1472
BceAI ACGGC 2 cut(s) 903, 1257
BciT130I CCWGG 4 cut(s) 698, 834, 1461, 1683
BcoDI GTCTC 5 cut(s) 124, 703, 1178, 1437, 1727
BfaI CTAG 3 cut(s) 243, 1158, 1697
BfmI CTRYAG 2 cut(s) 1050, 1407
BfoI RGCGCY 1 cut(s) 396
BglI GCCNNNNNGGC 1 cut(s) 390
BglII AGATCT 1 cut(s) 859
BisI GCNGC 5 cut(s) 136, 510, 993, 1329, 1530
BlsI GCNGC 5 cut(s) 137, 511, 994, 1330, 1531
Bme1390I CCNGG 4 cut(s) 698, 834, 1461, 1683
Bme18I GGWCC 2 cut(s) 298, 798
BmeRI GACNNNNNGTC 1 cut(s) 1740
BmgT120I GGNCC 4 cut(s) 298, 383, 580, 798
BmiI GGNNCC 5 cut(s) 143, 394, 799, 800, 1119
BmrFI CCNGG 4 cut(s) 698, 834, 1461, 1683
BmsI GCATC 6 cut(s) 53, 467, 602, 1358, 1391, 1414
BpiI GAAGAC 2 cut(s) 177, 183
Bpu10I CCTNAGC 1 cut(s) 1428
BpuEI CTTGAG 3 cut(s) 393, 556, 922
BsaAI YACGTR 2 cut(s) 40, 389
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 2 cut(s) 124, 703
BsaJI CCNNGG 5 cut(s) 114, 224, 1029, 1332, 1728
BsaWI WCCGGW 2 cut(s) 363, 1162
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 703
Bse118I RCCGGY 1 cut(s) 138
Bse1I ACTGG 6 cut(s) 351, 494, 583, 933, 1170, 1606
Bse21I CCTNAGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 1679
BseAI TCCGGA 2 cut(s) 363, 1162
BseBI CCWGG 4 cut(s) 698, 834, 1461, 1683
BseDI CCNNGG 5 cut(s) 114, 224, 1029, 1332, 1728
BseGI GGATG 2 cut(s) 408, 593
BseLI CCNNNNNNNGG 2 cut(s) 403, 703
BseMI GCAATG 1 cut(s) 1679
BseMII CTCAG 3 cut(s) 444, 1682, 1713
BseNI ACTGG 6 cut(s) 351, 494, 583, 933, 1170, 1606
BseRI GAGGAG 3 cut(s) 39, 298, 553
BseSI GKGCMC 1 cut(s) 1242
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 3 cut(s) 521, 979, 1315
BseYI CCCAGC 1 cut(s) 613
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 8 cut(s) 113, 138, 384, 582, 761, 1169, 1272, 1433
BshNI GGYRCC 1 cut(s) 392
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 3 cut(s) 523, 658, 1242
BsiSI CCGG 3 cut(s) 139, 364, 1163
BslFI GGGAC 2 cut(s) 784, 1047
BslI CCNNNNNNNGG 2 cut(s) 403, 703
BsmAI GTCTC 5 cut(s) 124, 703, 1178, 1437, 1727
BsmFI GGGAC 2 cut(s) 784, 1047
BsnI GGCC 8 cut(s) 113, 138, 384, 582, 761, 1169, 1272, 1433
Bso31I GGTCTC 2 cut(s) 124, 703
Bsp1286I GDGCHC 3 cut(s) 523, 658, 1242
Bsp13I TCCGGA 2 cut(s) 363, 1162
Bsp1407I TGTACA 2 cut(s) 3, 68
Bsp143I GATC 2 cut(s) 859, 1497
Bsp19I CCATGG 2 cut(s) 1029, 1332
BspACI CCGC 4 cut(s) 29, 135, 660, 1530
BspANI GGCC 8 cut(s) 113, 138, 384, 582, 761, 1169, 1272, 1433
BspCNI CTCAG 3 cut(s) 445, 1681, 1714
BspEI TCCGGA 2 cut(s) 363, 1162
BspLI GGNNCC 5 cut(s) 143, 394, 799, 800, 1119
BspT107I GGYRCC 1 cut(s) 392
BspTNI GGTCTC 2 cut(s) 124, 703
BsrDI GCAATG 1 cut(s) 1679
BsrFI RCCGGY 1 cut(s) 138
BsrGI TGTACA 2 cut(s) 3, 68
BsrI ACTGG 6 cut(s) 351, 494, 583, 933, 1170, 1606
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 5 cut(s) 114, 224, 1029, 1332, 1728
BssMI GATC 2 cut(s) 859, 1497
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 4 cut(s) 114, 1029, 1332, 1728
Bst2UI CCWGG 4 cut(s) 698, 834, 1461, 1683
Bst4CI ACNGT 3 cut(s) 225, 782, 1738
Bst6I CTCTTC 1 cut(s) 963
BstACI GRCGYC 1 cut(s) 393
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstAUI TGTACA 2 cut(s) 3, 68
BstBAI YACGTR 2 cut(s) 40, 389
BstC8I GCNNGC 4 cut(s) 140, 886, 1047, 1242
BstDEI CTNAG 7 cut(s) 398, 453, 791, 1132, 1428, 1668, 1722
BstDSI CCRYGG 3 cut(s) 224, 1029, 1332
BstF5I GGATG 2 cut(s) 408, 593
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 3 cut(s) 395, 555, 696
BstKTI GATC 2 cut(s) 862, 1500
BstMAI GTCTC 5 cut(s) 124, 703, 1178, 1437, 1727
BstMBI GATC 2 cut(s) 859, 1497
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 7 cut(s) 252, 261, 390, 518, 767, 1355, 1683
BstNI CCWGG 4 cut(s) 698, 834, 1461, 1683
BstNSI RCATGY 1 cut(s) 1690
BstSCI CCNGG 4 cut(s) 696, 832, 1459, 1681
BstSFI CTRYAG 2 cut(s) 1050, 1407
BstSLI GKGCMC 1 cut(s) 1242
BstV1I GCAGC 3 cut(s) 521, 979, 1315
BstV2I GAAGAC 2 cut(s) 177, 183
BstX2I RGATCY 1 cut(s) 859
BstXI CCANNNNNNTGG 1 cut(s) 1323
BstYI RGATCY 1 cut(s) 859
BstZI CGGCCG 1 cut(s) 136
Bsu36I CCTNAGG 1 cut(s) 398
BsuRI GGCC 8 cut(s) 113, 138, 384, 582, 761, 1169, 1272, 1433
BtgI CCRYGG 3 cut(s) 224, 1029, 1332
BtsCI GGATG 2 cut(s) 408, 593
BtsIMutI CAGTG 1 cut(s) 926
Cac8I GCNNGC 4 cut(s) 140, 886, 1047, 1242
CaiI CAGNNNCTG 1 cut(s) 1633
CfoI GCGC 3 cut(s) 395, 555, 696
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 4 cut(s) 298, 383, 580, 798
Csp6I GTAC 6 cut(s) 4, 69, 100, 378, 1503, 1516
CviAII CATG 6 cut(s) 556, 1030, 1229, 1323, 1333, 1687
CviQI GTAC 6 cut(s) 4, 69, 100, 378, 1503, 1516
DdeI CTNAG 7 cut(s) 398, 453, 791, 1132, 1428, 1668, 1722
DinI GGCGCC 1 cut(s) 394
DpnI GATC 2 cut(s) 861, 1499
DpnII GATC 2 cut(s) 859, 1497
DriI GACNNNNNGTC 1 cut(s) 1740
EaeI YGGCCR 2 cut(s) 136, 1270
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 963
Eam1105I GACNNNNNGTC 1 cut(s) 1740
EarI CTCTTC 1 cut(s) 963
EciI GGCGGA 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 656
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 4 cut(s) 114, 1029, 1332, 1728
Eco147I AGGCCT 1 cut(s) 1433
Eco24I GRGCYC 1 cut(s) 658
Eco31I GGTCTC 2 cut(s) 124, 703
Eco47I GGWCC 2 cut(s) 298, 798
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 656
Eco57I CTGAAG 2 cut(s) 994, 1383
Eco72I CACGTG 2 cut(s) 40, 389
Eco81I CCTNAGG 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 298, 798
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 4 cut(s) 696, 832, 1459, 1681
EcoT14I CCWWGG 4 cut(s) 114, 1029, 1332, 1728
EcoT38I GRGCYC 1 cut(s) 658
EgeI GGCGCC 1 cut(s) 394
EheI GGCGCC 1 cut(s) 394
ErhI CCWWGG 4 cut(s) 114, 1029, 1332, 1728
FaeI CATG 6 cut(s) 559, 1033, 1232, 1326, 1336, 1690
FalI AAGNNNNNCTT 2 cut(s) 907, 939
FaqI GGGAC 2 cut(s) 784, 1047
FatI CATG 6 cut(s) 555, 1029, 1228, 1322, 1332, 1686
FauI CCCGC 1 cut(s) 667
Fnu4HI GCNGC 5 cut(s) 136, 510, 993, 1329, 1530
FokI GGATG 2 cut(s) 415, 580
FriOI GRGCYC 1 cut(s) 658
Fsp4HI GCNGC 5 cut(s) 136, 510, 993, 1329, 1530
FspBI CTAG 3 cut(s) 243, 1158, 1697
GlaI GCGC 3 cut(s) 394, 554, 695
GluI GCNGC 5 cut(s) 136, 510, 993, 1329, 1530
GsaI CCCAGC 1 cut(s) 617
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 8 cut(s) 113, 138, 384, 582, 761, 1169, 1272, 1433
HapII CCGG 3 cut(s) 139, 364, 1163
HhaI GCGC 3 cut(s) 395, 555, 696
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 6 cut(s) 559, 1033, 1232, 1326, 1336, 1690
Hin6I GCGC 3 cut(s) 393, 553, 694
HinP1I GCGC 3 cut(s) 393, 553, 694
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 1222
HpaII CCGG 3 cut(s) 139, 364, 1163
HphI GGTGA 1 cut(s) 1061
Hpy166II GTNNAC 6 cut(s) 739, 778, 1148, 1208, 1240, 1505
Hpy188I TCNGA 3 cut(s) 639, 864, 1594
Hpy188III TCNNGA 9 cut(s) 320, 364, 826, 901, 908, 1012, 1163, 1286, 1697
Hpy8I GTNNAC 6 cut(s) 739, 778, 1148, 1208, 1240, 1505
HpyAV CCTTC 5 cut(s) 94, 253, 326, 937, 1018
HpyCH4III ACNGT 3 cut(s) 225, 782, 1738
HpyCH4IV ACGT 2 cut(s) 39, 388
HpyF10VI GCNNNNNNNGC 7 cut(s) 252, 261, 390, 518, 767, 1355, 1683
HpyF3I CTNAG 7 cut(s) 398, 453, 791, 1132, 1428, 1668, 1722
HpySE526I ACGT 2 cut(s) 39, 388
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 6 cut(s) 559, 1033, 1232, 1326, 1336, 1690
HspAI GCGC 3 cut(s) 393, 553, 694
KasI GGCGCC 1 cut(s) 392
KflI GGGWCCC 1 cut(s) 798
Kpn2I TCCGGA 2 cut(s) 363, 1162
KroI GCCGGC 1 cut(s) 138
KroNI GCCGGC 1 cut(s) 140
Kzo9I GATC 2 cut(s) 859, 1497
LmnI GCTCC 5 cut(s) 97, 147, 560, 661, 1060
Lsp1109I GCAGC 3 cut(s) 521, 979, 1315
LweI GCATC 6 cut(s) 53, 467, 602, 1358, 1391, 1414
MaeI CTAG 3 cut(s) 243, 1158, 1697
MaeII ACGT 2 cut(s) 39, 388
MaeIII GTNAC 2 cut(s) 281, 1629
MalI GATC 2 cut(s) 861, 1499
MboI GATC 2 cut(s) 859, 1497
MboII GAAGA 6 cut(s) 177, 183, 818, 854, 980, 1376
MfeI CAATTG 4 cut(s) 504, 714, 750, 1353
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 3 cut(s) 523, 658, 1242
Mly113I GGCGCC 1 cut(s) 393
MlyI GAGTC 5 cut(s) 913, 1013, 1029, 1130, 1733
MroI TCCGGA 2 cut(s) 363, 1162
MroNI GCCGGC 1 cut(s) 138
MroXI GAANNNNTTC 2 cut(s) 263, 1455
MseI TTAA 7 cut(s) 218, 374, 573, 1094, 1188, 1656, 1704
MslI CAYNNNNRTG 2 cut(s) 1233, 1321
MspI CCGG 3 cut(s) 139, 364, 1163
MspR9I CCNGG 4 cut(s) 698, 834, 1461, 1683
MunI CAATTG 4 cut(s) 504, 714, 750, 1353
MvaI CCWGG 4 cut(s) 698, 834, 1461, 1683
MwoI GCNNNNNNNGC 7 cut(s) 252, 261, 390, 518, 767, 1355, 1683
NaeI GCCGGC 1 cut(s) 140
NarI GGCGCC 1 cut(s) 393
NcoI CCATGG 2 cut(s) 1029, 1332
NdeII GATC 2 cut(s) 859, 1497
NgoMIV GCCGGC 1 cut(s) 138
NlaIII CATG 6 cut(s) 559, 1033, 1232, 1326, 1336, 1690
NlaIV GGNNCC 5 cut(s) 143, 394, 799, 800, 1119
NmeAIII GCCGAG 2 cut(s) 57, 101
NspI RCATGY 1 cut(s) 1690
PceI AGGCCT 1 cut(s) 1433
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PdiI GCCGGC 1 cut(s) 140
PdmI GAANNNNTTC 2 cut(s) 263, 1455
PfeI GAWTC 8 cut(s) 76, 679, 956, 1296, 1373, 1393, 1595, 1611
PflMI CCANNNNNTGG 1 cut(s) 703
PfoI TCCNGGA 1 cut(s) 1459
PkrI GCNGC 5 cut(s) 137, 511, 994, 1330, 1531
PleI GAGTC 5 cut(s) 912, 1012, 1029, 1130, 1732
PluTI GGCGCC 1 cut(s) 396
PmaCI CACGTG 2 cut(s) 40, 389
PmlI CACGTG 2 cut(s) 40, 389
PpsI GAGTC 5 cut(s) 912, 1012, 1029, 1130, 1732
Ppu21I YACGTR 2 cut(s) 40, 389
PpuMI RGGWCCY 2 cut(s) 298, 798
PsiI TTATAA 1 cut(s) 818
Psp124BI GAGCTC 1 cut(s) 658
Psp5II RGGWCCY 2 cut(s) 298, 798
Psp6I CCWGG 4 cut(s) 696, 832, 1459, 1681
PspCI CACGTG 2 cut(s) 40, 389
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 4 cut(s) 696, 832, 1459, 1681
PspN4I GGNNCC 5 cut(s) 143, 394, 799, 800, 1119
PspPI GGNCC 4 cut(s) 298, 383, 580, 798
PspPPI RGGWCCY 2 cut(s) 298, 798
PsrI GAACNNNNNNTAC 2 cut(s) 196, 228
PstNI CAGNNNCTG 1 cut(s) 1633
PsuI RGATCY 1 cut(s) 859
RsaI GTAC 6 cut(s) 5, 70, 101, 379, 1504, 1517
RsaNI GTAC 6 cut(s) 4, 69, 100, 378, 1503, 1516
RseI CAYNNNNRTG 2 cut(s) 1233, 1321
SacI GAGCTC 1 cut(s) 658
SaqAI TTAA 7 cut(s) 218, 374, 573, 1094, 1188, 1656, 1704
SatI GCNGC 5 cut(s) 136, 510, 993, 1329, 1530
Sau3AI GATC 2 cut(s) 859, 1497
Sau96I GGNCC 4 cut(s) 298, 383, 580, 798
SchI GAGTC 5 cut(s) 913, 1013, 1029, 1130, 1733
ScrFI CCNGG 4 cut(s) 698, 834, 1461, 1683
SduI GDGCHC 3 cut(s) 523, 658, 1242
SfaNI GCATC 6 cut(s) 53, 467, 602, 1358, 1391, 1414
SfcI CTRYAG 2 cut(s) 1050, 1407
SfoI GGCGCC 1 cut(s) 394
SinI GGWCC 2 cut(s) 298, 798
SmiMI CAYNNNNRTG 2 cut(s) 1233, 1321
SmlI CTYRAG 3 cut(s) 408, 535, 901
SmoI CTYRAG 3 cut(s) 408, 535, 901
SseBI AGGCCT 1 cut(s) 1433
SsiI CCGC 4 cut(s) 29, 135, 660, 1530
SspDI GGCGCC 1 cut(s) 392
SspI AATATT 1 cut(s) 690
SspMI CTAG 3 cut(s) 243, 1158, 1697
SstI GAGCTC 1 cut(s) 658
StuI AGGCCT 1 cut(s) 1433
StyD4I CCNGG 4 cut(s) 696, 832, 1459, 1681
StyI CCWWGG 4 cut(s) 114, 1029, 1332, 1728
TaaI ACNGT 3 cut(s) 225, 782, 1738
TaiI ACGT 2 cut(s) 42, 391
TaqI TCGA 1 cut(s) 257
TatI WGTACW 4 cut(s) 3, 68, 99, 377
TauI GCSGC 2 cut(s) 138, 1532
TfiI GAWTC 8 cut(s) 76, 679, 956, 1296, 1373, 1393, 1595, 1611
Tru1I TTAA 7 cut(s) 218, 374, 573, 1094, 1188, 1656, 1704
Tru9I TTAA 7 cut(s) 218, 374, 573, 1094, 1188, 1656, 1704
TscAI CASTG 1 cut(s) 933
TseI GCWGC 3 cut(s) 509, 992, 1328
TspDTI ATGAA 5 cut(s) 183, 251, 472, 1386, 1559
TspRI CASTG 1 cut(s) 933
Van91I CCANNNNNTGG 1 cut(s) 703
VneI GTGCAC 1 cut(s) 1238
VpaK11BI GGWCC 2 cut(s) 298, 798
XapI RAATTY 7 cut(s) 238, 479, 632, 865, 1089, 1455, 1762
XbaI TCTAGA 1 cut(s) 1696
XceI RCATGY 1 cut(s) 1690
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XmnI GAANNNNTTC 2 cut(s) 263, 1455
XspI CTAG 3 cut(s) 243, 1158, 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.