Rh1CG358300

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006647
Physical Location & Seq
Forward (+)
63217899 .. 63224630
6732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG358300.1

Sequence Viewer

Length: 1218 bp
ATGGCCTTGGTCTCGGCTGTGAGAGGCGGCCGGCTCCCGTCATCATTACATCATCATCATCATCTGGGTTCGTCTTCGTCTTCATCACTAGGTAGCTGGGGTTCGTTAAGACCGTGGAAGCAGATGAATTCTAGCTCAAAAAGCTCGAAGGGCTTCACTCTCTTGGCTCGTTACTCACAAACACAGGACCTTTTCTCTTCTCGCCTTCAAGATAGTATGGAAAACTTGCCTAAACTGGTGGAGGATATTGTCCGGACATCATTAAGTACAGGCCCACGTGGCGCCCTAAGGATGGCTCAAGGTATTCAAGCATTTATTGGTGTTGGTGGGGAGTGGCTTACTGAGGCATCAAAGTCAGCAAATTCATCTACTGGCTTACCAACCCAATTGCAGCTTGGAGTGCTCTCCCCCGTTTACTTGAGGAGATTGTTTGAGCGCATGGGAGCAACTTATATTAAATTGGGCCAGTTCATTGCATCCGCACCAACATTGTTCCCAGCAGACTATGTCGAGGAATTTCAGAACTGCTTTGACAGAGCTCCCGCAATACCTTTTGTGGAGATTCAACAAATATTGCGCCAGGAATTGGGGAGACCAATTGAAAGTGTATTTGAGTATGTTGACCCAACTCCAATTGCTTCGGCCTCAATAGCACAAGTTCACGGTGCAAGGCTAAGAGGGTCCCAAGAAGATGTGGTTATAAAGGTCTTGAAACCCGGAATAGAAGATATGTTGGTTGCAGATCTGAATTTTATTTACATAGTTGCTCGCATTTTGGAGTTCTTGAGTCCTGACATAAGCCGTGCTTCACTGGTTGGTATCGTGAAGGACATACGGGAATCTATGCTTGAAGAGGTTGACTTCTACAAGGAGGCTGCAAATGTTGAGTCCTTCAGGAGATATTTAGAAGCCATGGGACTCACAAGGCAGGCTACAGCTCCAAGAGTGTATCACCAATGCAGCACAAAACGAATTTTAACAATGGAGAGGCTATATGGGGTTCCTCTTACTGACTTAGACTCTATAAGTTCACTTGTTTCTAGTCCAGAGGCCAGTCTCATAACTGCCCTTAATGTGTGGTTTGGCAGTTTACTTGCCTGTGAAAGCTTCCATGCAGATGTGCACGCTGGAAATCTGTGGTTGTTGCGTGATGGCCGTATTGGGTTTCTTGACTTTGGTATGTCCTTATCCATTACATTTACCCATGGCTTGGTGTAG

Protein Analysis

405

Amino Acids

44.67

Weight (kDa)

6.57

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 177 - 398 2.4e-56 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 701
AccB1I GGYRCC 1 cut(s) 281
AccB7I CCANNNNNTGG 2 cut(s) 586, 1210
AccIII TCCGGA 1 cut(s) 252
AciI CCGC 3 cut(s) 27, 480, 543
AcoI YGGCCR 2 cut(s) 28, 1153
AcsI RAATTY 5 cut(s) 127, 361, 515, 748, 972
AcuI CTGAAG 1 cut(s) 877
AcvI CACGTG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 3 cut(s) 292, 586, 1210
AgsI TTSAA 6 cut(s) 209, 308, 566, 602, 712, 851
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 7 cut(s) 96, 135, 144, 394, 539, 938, 1107
AluI AGCT 7 cut(s) 96, 135, 144, 394, 539, 938, 1107
Alw21I GWGCWC 3 cut(s) 405, 541, 1125
Alw26I GTCTC 3 cut(s) 16, 586, 1061
Alw44I GTGCAC 1 cut(s) 1121
Aor13HI TCCGGA 1 cut(s) 252
AoxI GGCC 7 cut(s) 3, 28, 271, 463, 642, 1050, 1153
ApaLI GTGCAC 1 cut(s) 1121
ApeKI GCWGC 3 cut(s) 391, 875, 960
ApoI RAATTY 5 cut(s) 127, 361, 515, 748, 972
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 3 cut(s) 284, 438, 579
AspS9I GGNCC 4 cut(s) 187, 272, 463, 681
AsuC2I CCSGG 1 cut(s) 717
AsuHPI GGTGA 1 cut(s) 944
AvaII GGWCC 2 cut(s) 187, 681
AxyI CCTNAGG 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 1125
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 541
BbrPI CACGTG 1 cut(s) 278
BbsI GAAGAC 2 cut(s) 66, 72
Bbv12I GWGCWC 3 cut(s) 405, 541, 1125
BbvI GCAGC 3 cut(s) 403, 862, 972
BccI CCATC 2 cut(s) 286, 1145
BceAI ACGGC 2 cut(s) 786, 1140
BciT130I CCWGG 1 cut(s) 581
BcnI CCSGG 1 cut(s) 717
BcoDI GTCTC 3 cut(s) 16, 586, 1061
BfaI CTAG 3 cut(s) 89, 132, 1041
BfmI CTRYAG 1 cut(s) 933
BfoI RGCGCY 1 cut(s) 285
BglI GCCNNNNNGGC 1 cut(s) 279
BglII AGATCT 1 cut(s) 742
BisI GCNGC 4 cut(s) 28, 392, 876, 961
BlsI GCNGC 4 cut(s) 29, 393, 877, 962
Bme1390I CCNGG 2 cut(s) 581, 717
Bme18I GGWCC 2 cut(s) 187, 681
BmgT120I GGNCC 4 cut(s) 187, 272, 463, 681
BmiI GGNNCC 5 cut(s) 35, 283, 682, 683, 1002
BmrFI CCNGG 2 cut(s) 581, 717
BmsI GCATC 2 cut(s) 356, 485
BpiI GAAGAC 2 cut(s) 66, 72
BpuEI CTTGAG 3 cut(s) 282, 439, 805
BpuMI CCSGG 1 cut(s) 717
BsaAI YACGTR 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 2 cut(s) 16, 586
BsaJI CCNNGG 4 cut(s) 6, 113, 912, 1204
BsaWI WCCGGW 1 cut(s) 252
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 586, 1210
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 5 cut(s) 240, 376, 466, 816, 1053
Bse21I CCTNAGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 252
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 4 cut(s) 6, 113, 912, 1204
BseGI GGATG 2 cut(s) 297, 476
BseLI CCNNNNNNNGG 3 cut(s) 292, 586, 1210
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 5 cut(s) 240, 376, 466, 816, 1053
BseRI GAGGAG 1 cut(s) 436
BseSI GKGCMC 1 cut(s) 1125
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 3 cut(s) 403, 862, 972
BseYI CCCAGC 2 cut(s) 96, 496
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 7 cut(s) 5, 30, 273, 465, 644, 1052, 1155
BshNI GGYRCC 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 3 cut(s) 405, 541, 1125
BsiSI CCGG 3 cut(s) 31, 253, 717
BslFI GGGAC 2 cut(s) 667, 930
BslI CCNNNNNNNGG 3 cut(s) 292, 586, 1210
BsmAI GTCTC 3 cut(s) 16, 586, 1061
BsmFI GGGAC 2 cut(s) 667, 930
BsnI GGCC 7 cut(s) 5, 30, 273, 465, 644, 1052, 1155
Bso31I GGTCTC 2 cut(s) 16, 586
Bsp1286I GDGCHC 3 cut(s) 405, 541, 1125
Bsp13I TCCGGA 1 cut(s) 252
Bsp143I GATC 1 cut(s) 742
Bsp19I CCATGG 2 cut(s) 912, 1204
BspACI CCGC 3 cut(s) 27, 480, 543
BspANI GGCC 7 cut(s) 5, 30, 273, 465, 644, 1052, 1155
BspCNI CTCAG 1 cut(s) 334
BspEI TCCGGA 1 cut(s) 252
BspLI GGNNCC 5 cut(s) 35, 283, 682, 683, 1002
BspT107I GGYRCC 1 cut(s) 281
BspTNI GGTCTC 2 cut(s) 16, 586
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 5 cut(s) 240, 376, 466, 816, 1053
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 4 cut(s) 6, 113, 912, 1204
BssMI GATC 1 cut(s) 742
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 3 cut(s) 6, 912, 1204
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 2 cut(s) 114, 665
Bst6I CTCTTC 2 cut(s) 202, 846
BstACI GRCGYC 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 4 cut(s) 32, 769, 930, 1125
BstDEI CTNAG 4 cut(s) 287, 342, 674, 1015
BstDSI CCRYGG 3 cut(s) 113, 912, 1204
BstF5I GGATG 2 cut(s) 297, 476
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 3 cut(s) 284, 438, 579
BstKTI GATC 1 cut(s) 745
BstMAI GTCTC 3 cut(s) 16, 586, 1061
BstMBI GATC 1 cut(s) 742
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 5 cut(s) 141, 150, 279, 400, 650
BstNI CCWGG 1 cut(s) 581
BstSCI CCNGG 2 cut(s) 579, 715
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 1 cut(s) 1125
BstV1I GCAGC 3 cut(s) 403, 862, 972
BstV2I GAAGAC 2 cut(s) 66, 72
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BstZI CGGCCG 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 287
BsuRI GGCC 7 cut(s) 5, 30, 273, 465, 644, 1052, 1155
BtgI CCRYGG 3 cut(s) 113, 912, 1204
BtsCI GGATG 2 cut(s) 297, 476
BtsIMutI CAGTG 1 cut(s) 809
Cac8I GCNNGC 4 cut(s) 32, 769, 930, 1125
CfoI GCGC 3 cut(s) 284, 438, 579
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 187, 272, 463, 681
Csp6I GTAC 1 cut(s) 267
CviAII CATG 4 cut(s) 439, 913, 1112, 1205
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 4 cut(s) 287, 342, 674, 1015
DinI GGCGCC 1 cut(s) 283
DpnI GATC 1 cut(s) 744
DpnII GATC 1 cut(s) 742
EaeI YGGCCR 2 cut(s) 28, 1153
EagI CGGCCG 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 202, 846
EarI CTCTTC 2 cut(s) 202, 846
Ecl136II GAGCTC 1 cut(s) 539
EclXI CGGCCG 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 6, 912, 1204
Eco24I GRGCYC 1 cut(s) 541
Eco31I GGTCTC 2 cut(s) 16, 586
Eco47I GGWCC 2 cut(s) 187, 681
Eco52I CGGCCG 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 539
Eco57I CTGAAG 1 cut(s) 877
Eco72I CACGTG 1 cut(s) 278
Eco81I CCTNAGG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 539
EcoO109I RGGNCCY 2 cut(s) 187, 681
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 3 cut(s) 6, 912, 1204
EcoT38I GRGCYC 1 cut(s) 541
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 3 cut(s) 6, 912, 1204
FaeI CATG 4 cut(s) 442, 916, 1115, 1208
FalI AAGNNNNNCTT 2 cut(s) 790, 822
FaqI GGGAC 2 cut(s) 667, 930
FatI CATG 4 cut(s) 438, 912, 1111, 1204
FauI CCCGC 1 cut(s) 550
Fnu4HI GCNGC 4 cut(s) 28, 392, 876, 961
FokI GGATG 2 cut(s) 304, 463
FriOI GRGCYC 1 cut(s) 541
Fsp4HI GCNGC 4 cut(s) 28, 392, 876, 961
FspBI CTAG 3 cut(s) 89, 132, 1041
GlaI GCGC 3 cut(s) 283, 437, 578
GluI GCNGC 4 cut(s) 28, 392, 876, 961
GsaI CCCAGC 2 cut(s) 100, 500
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 7 cut(s) 5, 30, 273, 465, 644, 1052, 1155
HapII CCGG 3 cut(s) 31, 253, 717
HhaI GCGC 3 cut(s) 284, 438, 579
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 4 cut(s) 442, 916, 1115, 1208
Hin6I GCGC 3 cut(s) 282, 436, 577
HinP1I GCGC 3 cut(s) 282, 436, 577
HincII GTYRAC 2 cut(s) 622, 859
HindII GTYRAC 2 cut(s) 622, 859
HindIII AAGCTT 1 cut(s) 1105
HinfI GANTC 6 cut(s) 562, 787, 839, 887, 918, 1019
HpaII CCGG 3 cut(s) 31, 253, 717
HphI GGTGA 1 cut(s) 944
Hpy166II GTNNAC 7 cut(s) 415, 622, 661, 859, 1031, 1091, 1123
Hpy188I TCNGA 2 cut(s) 522, 747
Hpy188III TCNNGA 9 cut(s) 209, 253, 709, 784, 791, 823, 895, 1046, 1169
Hpy8I GTNNAC 7 cut(s) 415, 622, 661, 859, 1031, 1091, 1123
HpyAV CCTTC 4 cut(s) 142, 215, 820, 901
HpyCH4III ACNGT 2 cut(s) 114, 665
HpyCH4IV ACGT 1 cut(s) 277
HpyCH4V TGCA 8 cut(s) 391, 476, 668, 740, 878, 960, 1115, 1123
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 150, 279, 400, 650
HpyF3I CTNAG 4 cut(s) 287, 342, 674, 1015
HpySE526I ACGT 1 cut(s) 277
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 4 cut(s) 442, 916, 1115, 1208
HspAI GCGC 3 cut(s) 282, 436, 577
KasI GGCGCC 1 cut(s) 281
KflI GGGWCCC 1 cut(s) 681
Kpn2I TCCGGA 1 cut(s) 252
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Kzo9I GATC 1 cut(s) 742
LmnI GCTCC 4 cut(s) 39, 443, 544, 943
Lsp1109I GCAGC 3 cut(s) 403, 862, 972
LweI GCATC 2 cut(s) 356, 485
MaeI CTAG 3 cut(s) 89, 132, 1041
MaeII ACGT 1 cut(s) 277
MaeIII GTNAC 1 cut(s) 170
MalI GATC 1 cut(s) 744
MboI GATC 1 cut(s) 742
MboII GAAGA 6 cut(s) 66, 72, 189, 701, 737, 863
MfeI CAATTG 3 cut(s) 386, 597, 633
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 3 cut(s) 405, 541, 1125
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 4 cut(s) 796, 896, 912, 1013
MroI TCCGGA 1 cut(s) 252
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 5 cut(s) 107, 263, 456, 977, 1071
MslI CAYNNNNRTG 1 cut(s) 1116
MspI CCGG 3 cut(s) 31, 253, 717
MspR9I CCNGG 2 cut(s) 581, 717
MunI CAATTG 3 cut(s) 386, 597, 633
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 5 cut(s) 141, 150, 279, 400, 650
NaeI GCCGGC 1 cut(s) 32
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 717
NcoI CCATGG 2 cut(s) 912, 1204
NdeII GATC 1 cut(s) 742
NgoMIV GCCGGC 1 cut(s) 30
NlaIII CATG 4 cut(s) 442, 916, 1115, 1208
NlaIV GGNNCC 5 cut(s) 35, 283, 682, 683, 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 562, 839
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 2 cut(s) 586, 1210
PkrI GCNGC 4 cut(s) 29, 393, 877, 962
PleI GAGTC 4 cut(s) 795, 895, 912, 1013
PluTI GGCGCC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 278
PmlI CACGTG 1 cut(s) 278
PpsI GAGTC 4 cut(s) 795, 895, 912, 1013
Ppu21I YACGTR 1 cut(s) 278
PpuMI RGGWCCY 2 cut(s) 187, 681
PsiI TTATAA 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 541
Psp5II RGGWCCY 2 cut(s) 187, 681
Psp6I CCWGG 1 cut(s) 579
PspCI CACGTG 1 cut(s) 278
PspFI CCCAGC 2 cut(s) 96, 496
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 5 cut(s) 35, 283, 682, 683, 1002
PspPI GGNCC 4 cut(s) 187, 272, 463, 681
PspPPI RGGWCCY 2 cut(s) 187, 681
PsrI GAACNNNNNNTAC 2 cut(s) 85, 117
PsuI RGATCY 1 cut(s) 742
PsyI GACNNNGTC 1 cut(s) 506
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
RseI CAYNNNNRTG 1 cut(s) 1116
SacI GAGCTC 1 cut(s) 541
SaqAI TTAA 5 cut(s) 107, 263, 456, 977, 1071
SatI GCNGC 4 cut(s) 28, 392, 876, 961
Sau3AI GATC 1 cut(s) 742
Sau96I GGNCC 4 cut(s) 187, 272, 463, 681
SchI GAGTC 4 cut(s) 796, 896, 912, 1013
ScrFI CCNGG 2 cut(s) 581, 717
SduI GDGCHC 3 cut(s) 405, 541, 1125
SfaNI GCATC 2 cut(s) 356, 485
SfcI CTRYAG 1 cut(s) 933
SfoI GGCGCC 1 cut(s) 283
SinI GGWCC 2 cut(s) 187, 681
SmiMI CAYNNNNRTG 1 cut(s) 1116
SmlI CTYRAG 3 cut(s) 297, 418, 784
SmoI CTYRAG 3 cut(s) 297, 418, 784
SsiI CCGC 3 cut(s) 27, 480, 543
SspDI GGCGCC 1 cut(s) 281
SspI AATATT 1 cut(s) 573
SspMI CTAG 3 cut(s) 89, 132, 1041
SstI GAGCTC 1 cut(s) 541
StyD4I CCNGG 2 cut(s) 579, 715
StyI CCWWGG 3 cut(s) 6, 912, 1204
TaaI ACNGT 2 cut(s) 114, 665
TaiI ACGT 1 cut(s) 280
TaqI TCGA 2 cut(s) 146, 510
TatI WGTACW 1 cut(s) 266
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 2 cut(s) 562, 839
Tru1I TTAA 5 cut(s) 107, 263, 456, 977, 1071
Tru9I TTAA 5 cut(s) 107, 263, 456, 977, 1071
TscAI CASTG 1 cut(s) 816
TseI GCWGC 3 cut(s) 391, 875, 960
TspDTI ATGAA 4 cut(s) 72, 140, 354, 460
TspRI CASTG 1 cut(s) 816
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 2 cut(s) 586, 1210
VneI GTGCAC 1 cut(s) 1121
VpaK11BI GGWCC 2 cut(s) 187, 681
XapI RAATTY 5 cut(s) 127, 361, 515, 748, 972
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 3 cut(s) 89, 132, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.