Rh1AG381500

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006647
Physical Location & Seq
Forward (+)
61315788 .. 61322637
6850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG381500.1

Sequence Viewer

Length: 1233 bp
ATGGCCTTGGTCTCGGCTGTGAGAGGCGGCCGGCTCCCGTCATCATTACATCATCATCATCATCATCTGGGTTCGTCTTCGTCTTCATCACTAGGTAGCTGGGGTTCGTTAAGACCGTGGAAGCAGATGAATTCTAGCTCAAAAAGCTCGAAGGGCTTCACTCTCTTGGCTCGTTACTCACAAACACAGGACCTTTTCTCTTCTCGCCTTCAAGATAGTATGGAAAACTTGCCTAAACTGGTGGAGGATATTGTCCGGACATCATTAAGTACAGGCCCACGTGGCGCCCTAAGGATGGCTCAAGGTATTCAAGCATTTATTGGTGTTGGTGGGGAGTGGCTTACTGAGGCATCAAAGTCAGCAAATTCATCTACTGGCTTACCAACCCAATTGCAGCTTGGAGTGCTCTCCCCCCTTTACTTGAGGAGATTGTTTGAGCGCATGGGAGCAACTTATATTAAATTGGGCCAGTTCATTGCATCCGCACCAACATTGTTCCCAGCAGACTATGTCGAGGAATTTCAGAACTGCTTTGACAGAGCTCCCGCAATACCTTTTGTGGATATTCAACAAATATTGCGCCAGGAATTGGGGAGACCAATTGAAAGTGTATTTGAGTATGTTGACCCAACTCCAATTGCTTCGGCCTCAATAGCACAAGTTCACGGTGCAAGGCTAAGAGGGTCCCAAGAAGATGTGGTTATAAAGGTCTTGAAACCCGGAATAGAAGATATGTTGGTTGCAGATCTGAATTTTATTTACATAGTTGCTCGCATTTTGGAGTTCTTGAGTCCTGACATAAGCCGTGCTTCACTGGTTGGTATCGTGAAGGACATACGGGAATCTATGCTTGAAGAGGTTGATTTCTACAAGGAGGCTGCAAATGTTGAGTCCTTCAGGAGATATTTAGAAGCCATGGGACTCACAAGGCAGGCTACAGCTCCAAGAGTGTATCACCAATGCAGCACAAAACGAATTTTAACAATGGAGAGGCTATATGGGGTTCCTCTTACTGACTTAGACTCTATAAGTTCACTTGTTTCTAGTCCGGAGGCCAGTCTCATAACTGCCCTTAATGTGTGGTTTGGCAGTTTACTTGCCTGTGAAAGCTTCCATGCAGATGTGCACGCTGGAAATCTGTGGTTGTTGCGTGATGGCCGTATTGGCTTTCTTGACTTTGGTATGTCCTCATCCATTACATTTACCCATGGCTTGGTGGAGTGGTTGAAATAG

Protein Analysis

410

Amino Acids

45.34

Weight (kDa)

6.61

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 178 - 400 1.4e-56 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 2 cut(s) 589, 1213
AccIII TCCGGA 2 cut(s) 255, 1048
AciI CCGC 3 cut(s) 27, 483, 546
AcoI YGGCCR 2 cut(s) 28, 1156
AcsI RAATTY 5 cut(s) 130, 364, 518, 751, 975
AcuI CTGAAG 1 cut(s) 880
AcvI CACGTG 1 cut(s) 281
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 271
AfiI CCNNNNNNNGG 3 cut(s) 295, 589, 1213
AgsI TTSAA 7 cut(s) 212, 311, 569, 605, 715, 854, 1228
AjnI CCWGG 1 cut(s) 582
AluBI AGCT 7 cut(s) 99, 138, 147, 397, 542, 941, 1110
AluI AGCT 7 cut(s) 99, 138, 147, 397, 542, 941, 1110
Alw21I GWGCWC 3 cut(s) 408, 544, 1128
Alw26I GTCTC 3 cut(s) 16, 589, 1064
Alw44I GTGCAC 1 cut(s) 1124
Aor13HI TCCGGA 2 cut(s) 255, 1048
AoxI GGCC 7 cut(s) 3, 28, 274, 466, 645, 1053, 1156
ApaLI GTGCAC 1 cut(s) 1124
ApeKI GCWGC 3 cut(s) 394, 878, 963
ApoI RAATTY 5 cut(s) 130, 364, 518, 751, 975
ArsI GACNNNNNNTTYG 2 cut(s) 875, 907
Asp700I GAANNNNTTC 1 cut(s) 155
AspLEI GCGC 3 cut(s) 287, 441, 582
AspS9I GGNCC 4 cut(s) 190, 275, 466, 684
AsuC2I CCSGG 1 cut(s) 720
AsuHPI GGTGA 1 cut(s) 947
AvaII GGWCC 2 cut(s) 190, 684
AxyI CCTNAGG 1 cut(s) 290
BaeGI GKGCMC 1 cut(s) 1128
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 544
BbrPI CACGTG 1 cut(s) 281
BbsI GAAGAC 2 cut(s) 69, 75
Bbv12I GWGCWC 3 cut(s) 408, 544, 1128
BbvI GCAGC 3 cut(s) 406, 865, 975
BccI CCATC 2 cut(s) 289, 1148
BceAI ACGGC 2 cut(s) 789, 1143
BciT130I CCWGG 1 cut(s) 584
BcnI CCSGG 1 cut(s) 720
BcoDI GTCTC 3 cut(s) 16, 589, 1064
BfaI CTAG 3 cut(s) 92, 135, 1044
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 1 cut(s) 288
BglI GCCNNNNNGGC 2 cut(s) 282, 1164
BglII AGATCT 1 cut(s) 745
BisI GCNGC 4 cut(s) 28, 395, 879, 964
BlsI GCNGC 4 cut(s) 29, 396, 880, 965
Bme1390I CCNGG 2 cut(s) 584, 720
Bme18I GGWCC 2 cut(s) 190, 684
BmgT120I GGNCC 4 cut(s) 190, 275, 466, 684
BmiI GGNNCC 5 cut(s) 35, 286, 685, 686, 1005
BmrFI CCNGG 2 cut(s) 584, 720
BmsI GCATC 2 cut(s) 359, 488
BpiI GAAGAC 2 cut(s) 69, 75
BpuEI CTTGAG 3 cut(s) 285, 442, 808
BpuMI CCSGG 1 cut(s) 720
BsaAI YACGTR 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 285
BsaI GGTCTC 2 cut(s) 16, 589
BsaJI CCNNGG 4 cut(s) 6, 116, 915, 1207
BsaWI WCCGGW 2 cut(s) 255, 1048
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 589, 1213
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 5 cut(s) 243, 379, 469, 819, 1056
Bse21I CCTNAGG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 474
BseAI TCCGGA 2 cut(s) 255, 1048
BseBI CCWGG 1 cut(s) 584
BseDI CCNNGG 4 cut(s) 6, 116, 915, 1207
BseGI GGATG 3 cut(s) 300, 479, 1190
BseLI CCNNNNNNNGG 3 cut(s) 295, 589, 1213
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 5 cut(s) 243, 379, 469, 819, 1056
BseRI GAGGAG 1 cut(s) 439
BseSI GKGCMC 1 cut(s) 1128
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 3 cut(s) 406, 865, 975
BseYI CCCAGC 2 cut(s) 99, 499
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 7 cut(s) 5, 30, 276, 468, 647, 1055, 1158
BshNI GGYRCC 1 cut(s) 284
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 3 cut(s) 408, 544, 1128
BsiSI CCGG 4 cut(s) 31, 256, 720, 1049
BslFI GGGAC 2 cut(s) 670, 933
BslI CCNNNNNNNGG 3 cut(s) 295, 589, 1213
BsmAI GTCTC 3 cut(s) 16, 589, 1064
BsmFI GGGAC 2 cut(s) 670, 933
BsnI GGCC 7 cut(s) 5, 30, 276, 468, 647, 1055, 1158
Bso31I GGTCTC 2 cut(s) 16, 589
Bsp1286I GDGCHC 3 cut(s) 408, 544, 1128
Bsp13I TCCGGA 2 cut(s) 255, 1048
Bsp143I GATC 1 cut(s) 745
Bsp19I CCATGG 2 cut(s) 915, 1207
BspACI CCGC 3 cut(s) 27, 483, 546
BspANI GGCC 7 cut(s) 5, 30, 276, 468, 647, 1055, 1158
BspCNI CTCAG 1 cut(s) 337
BspEI TCCGGA 2 cut(s) 255, 1048
BspLI GGNNCC 5 cut(s) 35, 286, 685, 686, 1005
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 2 cut(s) 16, 589
BsrDI GCAATG 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 5 cut(s) 243, 379, 469, 819, 1056
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 4 cut(s) 6, 116, 915, 1207
BssMI GATC 1 cut(s) 745
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 3 cut(s) 6, 915, 1207
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 2 cut(s) 117, 668
Bst6I CTCTTC 2 cut(s) 205, 849
BstACI GRCGYC 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 281
BstC8I GCNNGC 4 cut(s) 32, 772, 933, 1128
BstDEI CTNAG 4 cut(s) 290, 345, 677, 1018
BstDSI CCRYGG 3 cut(s) 116, 915, 1207
BstF5I GGATG 3 cut(s) 300, 479, 1190
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 3 cut(s) 287, 441, 582
BstKTI GATC 1 cut(s) 748
BstMAI GTCTC 3 cut(s) 16, 589, 1064
BstMBI GATC 1 cut(s) 745
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 6 cut(s) 144, 153, 282, 403, 653, 1164
BstNI CCWGG 1 cut(s) 584
BstSCI CCNGG 2 cut(s) 582, 718
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 1128
BstV1I GCAGC 3 cut(s) 406, 865, 975
BstV2I GAAGAC 2 cut(s) 69, 75
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
BstZI CGGCCG 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 290
BsuRI GGCC 7 cut(s) 5, 30, 276, 468, 647, 1055, 1158
BtgI CCRYGG 3 cut(s) 116, 915, 1207
BtsCI GGATG 3 cut(s) 300, 479, 1190
BtsIMutI CAGTG 1 cut(s) 812
Cac8I GCNNGC 4 cut(s) 32, 772, 933, 1128
CfoI GCGC 3 cut(s) 287, 441, 582
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 190, 275, 466, 684
Csp6I GTAC 1 cut(s) 270
CviAII CATG 4 cut(s) 442, 916, 1115, 1208
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 4 cut(s) 290, 345, 677, 1018
DinI GGCGCC 1 cut(s) 286
DpnI GATC 1 cut(s) 747
DpnII GATC 1 cut(s) 745
EaeI YGGCCR 2 cut(s) 28, 1156
EagI CGGCCG 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 205, 849
EarI CTCTTC 2 cut(s) 205, 849
Ecl136II GAGCTC 1 cut(s) 542
EclXI CGGCCG 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 6, 915, 1207
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 2 cut(s) 16, 589
Eco47I GGWCC 2 cut(s) 190, 684
Eco52I CGGCCG 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 880
Eco72I CACGTG 1 cut(s) 281
Eco81I CCTNAGG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 542
EcoO109I RGGNCCY 2 cut(s) 190, 684
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 582
EcoT14I CCWWGG 3 cut(s) 6, 915, 1207
EcoT38I GRGCYC 1 cut(s) 544
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 3 cut(s) 6, 915, 1207
FaeI CATG 4 cut(s) 445, 919, 1118, 1211
FalI AAGNNNNNCTT 2 cut(s) 793, 825
FaqI GGGAC 2 cut(s) 670, 933
FatI CATG 4 cut(s) 441, 915, 1114, 1207
FauI CCCGC 1 cut(s) 553
Fnu4HI GCNGC 4 cut(s) 28, 395, 879, 964
FokI GGATG 3 cut(s) 307, 466, 1177
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 4 cut(s) 28, 395, 879, 964
FspBI CTAG 3 cut(s) 92, 135, 1044
GlaI GCGC 3 cut(s) 286, 440, 581
GluI GCNGC 4 cut(s) 28, 395, 879, 964
GsaI CCCAGC 2 cut(s) 103, 503
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 7 cut(s) 5, 30, 276, 468, 647, 1055, 1158
HapII CCGG 4 cut(s) 31, 256, 720, 1049
HhaI GCGC 3 cut(s) 287, 441, 582
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 4 cut(s) 445, 919, 1118, 1211
Hin6I GCGC 3 cut(s) 285, 439, 580
HinP1I GCGC 3 cut(s) 285, 439, 580
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 1108
HinfI GANTC 5 cut(s) 790, 842, 890, 921, 1022
HpaII CCGG 4 cut(s) 31, 256, 720, 1049
HphI GGTGA 1 cut(s) 947
Hpy166II GTNNAC 5 cut(s) 625, 664, 1034, 1094, 1126
Hpy188I TCNGA 2 cut(s) 525, 750
Hpy188III TCNNGA 9 cut(s) 212, 256, 712, 787, 794, 826, 898, 1049, 1172
Hpy8I GTNNAC 5 cut(s) 625, 664, 1034, 1094, 1126
HpyAV CCTTC 4 cut(s) 145, 218, 823, 904
HpyCH4III ACNGT 2 cut(s) 117, 668
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 8 cut(s) 394, 479, 671, 743, 881, 963, 1118, 1126
HpyF10VI GCNNNNNNNGC 6 cut(s) 144, 153, 282, 403, 653, 1164
HpyF3I CTNAG 4 cut(s) 290, 345, 677, 1018
HpySE526I ACGT 1 cut(s) 280
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 4 cut(s) 445, 919, 1118, 1211
HspAI GCGC 3 cut(s) 285, 439, 580
KasI GGCGCC 1 cut(s) 284
KflI GGGWCCC 1 cut(s) 684
Kpn2I TCCGGA 2 cut(s) 255, 1048
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Kzo9I GATC 1 cut(s) 745
LmnI GCTCC 4 cut(s) 39, 446, 547, 946
Lsp1109I GCAGC 3 cut(s) 406, 865, 975
LweI GCATC 2 cut(s) 359, 488
MaeI CTAG 3 cut(s) 92, 135, 1044
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 1 cut(s) 173
MalI GATC 1 cut(s) 747
MboI GATC 1 cut(s) 745
MboII GAAGA 6 cut(s) 69, 75, 192, 704, 740, 866
MfeI CAATTG 3 cut(s) 389, 600, 636
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 3 cut(s) 408, 544, 1128
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 4 cut(s) 799, 899, 915, 1016
MroI TCCGGA 2 cut(s) 255, 1048
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 155
MseI TTAA 5 cut(s) 110, 266, 459, 980, 1074
MslI CAYNNNNRTG 1 cut(s) 1119
MspI CCGG 4 cut(s) 31, 256, 720, 1049
MspR9I CCNGG 2 cut(s) 584, 720
MunI CAATTG 3 cut(s) 389, 600, 636
MvaI CCWGG 1 cut(s) 584
MwoI GCNNNNNNNGC 6 cut(s) 144, 153, 282, 403, 653, 1164
NaeI GCCGGC 1 cut(s) 32
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 1 cut(s) 720
NcoI CCATGG 2 cut(s) 915, 1207
NdeII GATC 1 cut(s) 745
NgoMIV GCCGGC 1 cut(s) 30
NlaIII CATG 4 cut(s) 445, 919, 1118, 1211
NlaIV GGNNCC 5 cut(s) 35, 286, 685, 686, 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 1 cut(s) 842
PflFI GACNNNGTC 1 cut(s) 509
PflMI CCANNNNNTGG 2 cut(s) 589, 1213
PkrI GCNGC 4 cut(s) 29, 396, 880, 965
PleI GAGTC 4 cut(s) 798, 898, 915, 1016
PluTI GGCGCC 1 cut(s) 288
PmaCI CACGTG 1 cut(s) 281
PmlI CACGTG 1 cut(s) 281
PpsI GAGTC 4 cut(s) 798, 898, 915, 1016
Ppu21I YACGTR 1 cut(s) 281
PpuMI RGGWCCY 2 cut(s) 190, 684
PsiI TTATAA 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 544
Psp5II RGGWCCY 2 cut(s) 190, 684
Psp6I CCWGG 1 cut(s) 582
PspCI CACGTG 1 cut(s) 281
PspFI CCCAGC 2 cut(s) 99, 499
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 5 cut(s) 35, 286, 685, 686, 1005
PspPI GGNCC 4 cut(s) 190, 275, 466, 684
PspPPI RGGWCCY 2 cut(s) 190, 684
PsrI GAACNNNNNNTAC 2 cut(s) 88, 120
PsuI RGATCY 1 cut(s) 745
PsyI GACNNNGTC 1 cut(s) 509
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
RseI CAYNNNNRTG 1 cut(s) 1119
SacI GAGCTC 1 cut(s) 544
SaqAI TTAA 5 cut(s) 110, 266, 459, 980, 1074
SatI GCNGC 4 cut(s) 28, 395, 879, 964
Sau3AI GATC 1 cut(s) 745
Sau96I GGNCC 4 cut(s) 190, 275, 466, 684
SchI GAGTC 4 cut(s) 799, 899, 915, 1016
ScrFI CCNGG 2 cut(s) 584, 720
SduI GDGCHC 3 cut(s) 408, 544, 1128
SfaNI GCATC 2 cut(s) 359, 488
SfcI CTRYAG 1 cut(s) 936
SfoI GGCGCC 1 cut(s) 286
SinI GGWCC 2 cut(s) 190, 684
SmiMI CAYNNNNRTG 1 cut(s) 1119
SmlI CTYRAG 3 cut(s) 300, 421, 787
SmoI CTYRAG 3 cut(s) 300, 421, 787
SsiI CCGC 3 cut(s) 27, 483, 546
SspDI GGCGCC 1 cut(s) 284
SspI AATATT 1 cut(s) 576
SspMI CTAG 3 cut(s) 92, 135, 1044
SstI GAGCTC 1 cut(s) 544
StyD4I CCNGG 2 cut(s) 582, 718
StyI CCWWGG 3 cut(s) 6, 915, 1207
TaaI ACNGT 2 cut(s) 117, 668
TaiI ACGT 1 cut(s) 283
TaqI TCGA 2 cut(s) 149, 513
TatI WGTACW 1 cut(s) 269
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 1 cut(s) 842
Tru1I TTAA 5 cut(s) 110, 266, 459, 980, 1074
Tru9I TTAA 5 cut(s) 110, 266, 459, 980, 1074
TscAI CASTG 1 cut(s) 819
TseI GCWGC 3 cut(s) 394, 878, 963
TspDTI ATGAA 4 cut(s) 75, 143, 357, 463
TspRI CASTG 1 cut(s) 819
Tth111I GACNNNGTC 1 cut(s) 509
Van91I CCANNNNNTGG 2 cut(s) 589, 1213
VneI GTGCAC 1 cut(s) 1124
VpaK11BI GGWCC 2 cut(s) 190, 684
XapI RAATTY 5 cut(s) 130, 364, 518, 751, 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 155
XspI CTAG 3 cut(s) 92, 135, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.