Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005461

Physical Location & Seq

Genomic Coordinates Reverse (-)

27838697 .. 27843414

Gene Structure 4718 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1178000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGAACGTGATGTAGATTGCTGTATTGAACCAGATGAATCTCCCCCTCTCCCGCACGCGCAGTTTGACGGCGGAGCCACGGCGGCGGCTTTGAGCTGCAGCCTCACCTTGGACCGCGTCGGGGAAGTCCTTCTCTTCTTAAACTCAGATGGGTTGTCTTGGAAATCGCTCGAGCCATTGGAAAATGATGACTCAACTTGTTTGGGCATCACATTTACTTCAAAGGTTGCAACCGAGATCAAATTTTCCGATGTGTATTCCGTTGAGTTGATCAGCCACGGTTTGATCCATGTACCAAAGGTTTCTAATGCTAGAAGATGCCTTTCTGGTCATCATGAATCTGAGTGCATGGTTTCCAGCAGTCAAAGACTCTGCCTTCTCTTTCGGGTCCTGGCTAAATATACGTTTGGTCATAATGATCTCCAAACATGTCAGATGTGGGTCGATCAGATTAATGCTTCTCTGGCCCTAGAACAGGGGCGACCAAAGAATCTATTGGTTTTTGTTCATCCAAAGAGTGGGAAGGGAAACGGCTGCAAAATTTGGGACTCGGTGGCTCCTATATTCACCCATGCTAAAGTGAAAACAAAGGTCATTGTGACCCAAAGGTCAGGGCACGCATATGATATGATGGCATCTATAGGTAACAAAGAGCTTGCTTCATATGACGGAGTTATAGCCATTGGCGGTGATGGTTTCTTCAATGAAATCCTCAATGGATTTCTTTCTTCAAGGCATAAAGCTCCATACCCGCCAACTCCTTCAGGTTTTCTTCCTTCTGATACATGTCATGACAGTTTCTGTGTTCATGATCCAAATGAAACAGTTATTGGAACTTCTTCTCAGAACATTGAGCAGTCTCCTCTCCTCCCAAGTCCAATCCATGATGGTTCGGGAGACCCAGTTATCAATGAAGATCCAGAGTTTCCCTTCCCCAATGAGCATTTCAGATTTGGAATTATTCCTGCTGGCTCTACCGATGCCATTGTGATGTGCACAACTGGTGCTCGAGATCCTATAACATCTACATTTCACATTGTCCTTGGGAAAAGGGTTTCCCTTGATGTAGCCCAAGTTGTAAGGTGGAAAACAACATCTACATCAAAGGTTGAACCTTTTGTGCGCTATGCAGCATCTTTTGCCGGATGTTCTATTTTTGTAGGTATGGATTTTATGGAGATGTCATTACTGAGAGTGAAAAATACCAGTTGGATGGGTCCGAAAAGATATGATTATGCAGGAACAAGGGTATTTTTAAAGCACCGGTCCTATGAGGCAGAAGTAGCATATATAGAAGTCAAACCAGAAGGAAAGTCAATTCCTGAAAGAGGAAGTTTGAGCGGTCGCAAGCGACCATTTTGGAGCCCAAATAAATCCGATCAAGCAATATGCCGTGTTAACTGCAAGGTCTGTAGCAGCACCACCCCTTGCTCATATCCAGGAGAAACAAAATGGTCCATATCAAAGGGAAAGTTTCTTAGCATTGGTGCCGCAGTAATTTCCAATAGAAATGAAAGAGCACCTGACGGTTTGGTGGCGGATGCACACCTTTCAGACGGTTTCATGCATCTCATATTGATCAAGGACTGCTCTCATGCTCTCTATCTATGGCATCTTACCCAGCTTGCAAAGAAGGGCGGAAACCCCTTCGATTTCAAGTTTGTTGAACACCACAAGACCACTGCTTTTACGTTCACATCTTCCGGCAACGAGAGTGTATGGAATTTGGACGGGGAACTCTTTCAAGCACACCAATTATCGGCGCAAGTATTTCGAGGCCTTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001727 GO:0001729 GO:0003674 GO:0003824 GO:0005488 GO:0005509 GO:0006629 GO:0006643 GO:0006665 GO:0006672 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008219 GO:0009987 GO:0010941 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0030258 GO:0034641 GO:0043067 GO:0043069 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0044238 GO:0044255 GO:0046834 GO:0046872 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00600 map00600

Pfam Domains

Protein Families

DAGK_cat

Protein Analysis

596

Amino Acids

65.46

Weight (kDa)

6.54

Isoelectric Point (pI)

43.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	165 - 263	1.4e-17	Diacylglycerol kinase catalytic domain
CERK_C	PF19280	480 - 564	2.7e-09	Ceramide kinase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51290 AT5G51290
fragaria_vesca	FvH4_7g27040 FvH4_7g27040
malus_domestica	MD01G1178000.v1.1 MD07G1250200.v1.1
prunus_persica	Prupe.2G273600_v2.0.a1 Prupe.2G273600_v2.0.a1 Prupe.2G273600_v2.0.a1
pyrus_communis	pycom01g18880
rosa_chinensis	RchiOBHm_Chr1g0337211 RchiOBHm_Chr1g0373861
rosa_laevigata	RLG00000026800 RLG00000029293
rosa_multiflora	Rmu_sc0002160.1_g000003 Rmu_sc0013735.1_g000011 Rmu_ssc0000206.1_g000002
rosa_roxburghii	Rroxscaffold_4G00283960 Rroxscaffold_4G00314670
rosa_rugosa	Rorug01G0132600.1 Rorug01G0379600
rosa_samantha	Rh1AG389300 Rh1BG353500 Rh1CG106300 Rh1CG366800 Rh1DG108300 Rh1DG115200 Rh1DG384500
rosa_wichuraiana	Rw1G034590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	607
AccB1I	GGYRCC	1 cut(s)	1487
AccB7I	CCANNNNNTGG	1 cut(s)	518
AccBSI	CCGCTC	1 cut(s)	1341
AccII	CGCG	2 cut(s)	59, 117
AclWI	GGATC	4 cut(s)	280, 806, 911, 1007
AcsI	RAATTY	3 cut(s)	242, 540, 1723
AcuI	CTGAAG	1 cut(s)	747
AfaI	GTAC	1 cut(s)	294
AfiI	CCNNNNNNNGG	6 cut(s)	109, 121, 475, 518, 1328, 1759
AflIII	ACRYGT	2 cut(s)	428, 785
AgeI	ACCGGT	1 cut(s)	1263
AgsI	TTSAA	8 cut(s)	29, 222, 703, 732, 1112, 1657, 1667, 1745
AjnI	CCWGG	2 cut(s)	390, 1438
AjuI	GAANNNNNNNTTGG	4 cut(s)	813, 845, 929, 961
AluBI	AGCT	4 cut(s)	96, 655, 743, 1624
AluI	AGCT	4 cut(s)	96, 655, 743, 1624
Alw21I	GWGCWC	3 cut(s)	998, 1009, 1522
Alw26I	GTCTC	2 cut(s)	864, 891
Alw44I	GTGCAC	1 cut(s)	994
AlwI	GGATC	4 cut(s)	280, 806, 911, 1007
AlwNI	CAGNNNCTG	1 cut(s)	801
Ama87I	CYCGRG	2 cut(s)	170, 1008
AoxI	GGCC	2 cut(s)	465, 1777
ApaLI	GTGCAC	1 cut(s)	994
ApeKI	GCWGC	5 cut(s)	96, 99, 534, 1130, 1416
ApoI	RAATTY	3 cut(s)	242, 540, 1723
AseI	ATTAAT	1 cut(s)	453
AsiGI	ACCGGT	1 cut(s)	1263
Asp700I	GAANNNNTTC	3 cut(s)	129, 838, 1740
AspLEI	GCGC	3 cut(s)	61, 1125, 1765
AspS9I	GGNCC	6 cut(s)	112, 388, 466, 1217, 1266, 1455
AsuHPI	GGTGA	3 cut(s)	97, 559, 701
AvaI	CYCGRG	2 cut(s)	170, 1008
AvaII	GGWCC	5 cut(s)	112, 388, 1217, 1266, 1455
BaeGI	GKGCMC	2 cut(s)	618, 998
BanI	GGYRCC	1 cut(s)	1487
BanII	GRGCYC	1 cut(s)	1367
Bbv12I	GWGCWC	3 cut(s)	998, 1009, 1522
BbvI	GCAGC	5 cut(s)	83, 111, 521, 1142, 1428
BccI	CCATC	5 cut(s)	143, 625, 686, 881, 1207
BceAI	ACGGC	4 cut(s)	85, 96, 547, 1377
BcgI	CGANNNNNNTGC	2 cut(s)	1754, 1788
BciT130I	CCWGG	2 cut(s)	392, 1440
BclI	TGATCA	2 cut(s)	270, 1578
BcoDI	GTCTC	2 cut(s)	864, 891
BfaI	CTAG	2 cut(s)	312, 470
BfmI	CTRYAG	3 cut(s)	97, 639, 1411
BglI	GCCNNNNNGGC	1 cut(s)	83
BisI	GCNGC	8 cut(s)	84, 87, 97, 100, 535, 1131, 1417, 1491
BlsI	GCNGC	8 cut(s)	85, 88, 98, 101, 536, 1132, 1418, 1492
Bme1390I	CCNGG	2 cut(s)	392, 1440
Bme18I	GGWCC	5 cut(s)	112, 388, 1217, 1266, 1455
BmeT110I	CYCGRG	2 cut(s)	170, 1008
BmgT120I	GGNCC	6 cut(s)	112, 388, 466, 1217, 1266, 1455
BmiI	GGNNCC	6 cut(s)	76, 389, 558, 1218, 1364, 1489
BmrFI	CCNGG	2 cut(s)	392, 1440
BmrI	ACTGGG	1 cut(s)	896
BmsI	GCATC	8 cut(s)	216, 308, 644, 970, 1142, 1531, 1576, 1621
BmuI	ACTGGG	1 cut(s)	896
BsaI	GGTCTC	1 cut(s)	891
BsaJI	CCNNGG	4 cut(s)	78, 108, 277, 1042
BsaWI	WCCGGW	1 cut(s)	1263
BsaXI	ACNNNNNCTCC	2 cut(s)	888, 918
Bsc4I	CCNNNNNNNGG	6 cut(s)	109, 121, 475, 518, 1328, 1759
Bse118I	RCCGGY	1 cut(s)	1263
Bse1I	ACTGG	3 cut(s)	902, 1006, 1206
BseBI	CCWGG	2 cut(s)	392, 1440
BseDI	CCNNGG	4 cut(s)	78, 108, 277, 1042
BseGI	GGATG	4 cut(s)	508, 1151, 1218, 1546
BseLI	CCNNNNNNNGG	6 cut(s)	109, 121, 475, 518, 1328, 1759
BseMII	CTCAG	4 cut(s)	159, 333, 857, 1181
BseNI	ACTGG	3 cut(s)	902, 1006, 1206
BseRI	GAGGAG	2 cut(s)	852, 857
BseSI	GKGCMC	2 cut(s)	618, 998
BseXI	GCAGC	5 cut(s)	83, 111, 521, 1142, 1428
BseYI	CCCAGC	1 cut(s)	1620
Bsh1236I	CGCG	2 cut(s)	59, 117
Bsh1285I	CGRYCG	1 cut(s)	1345
BshFI	GGCC	2 cut(s)	467, 1779
BshNI	GGYRCC	1 cut(s)	1487
BshTI	ACCGGT	1 cut(s)	1263
BsiEI	CGRYCG	1 cut(s)	1345
BsiHKAI	GWGCWC	3 cut(s)	998, 1009, 1522
BsiHKCI	CYCGRG	2 cut(s)	170, 1008
BsiSI	CCGG	3 cut(s)	1143, 1264, 1704
BslFI	GGGAC	1 cut(s)	560
BslI	CCNNNNNNNGG	6 cut(s)	109, 121, 475, 518, 1328, 1759
BsmAI	GTCTC	2 cut(s)	864, 891
BsmFI	GGGAC	1 cut(s)	560
BsnI	GGCC	2 cut(s)	467, 1779
Bso31I	GGTCTC	1 cut(s)	891
BsoBI	CYCGRG	2 cut(s)	170, 1008
Bsp1286I	GDGCHC	5 cut(s)	618, 998, 1009, 1367, 1522
BspANI	GGCC	2 cut(s)	467, 1779
BspCNI	CTCAG	4 cut(s)	158, 334, 856, 1182
BspFNI	CGCG	2 cut(s)	59, 117
BspHI	TCATGA	3 cut(s)	334, 790, 808
BspLI	GGNNCC	6 cut(s)	76, 389, 558, 1218, 1364, 1489
BspMAI	CTGCAG	1 cut(s)	101
BspPI	GGATC	4 cut(s)	280, 806, 911, 1007
BspT107I	GGYRCC	1 cut(s)	1487
BspTNI	GGTCTC	1 cut(s)	891
BsrBI	CCGCTC	1 cut(s)	1341
BsrFI	RCCGGY	1 cut(s)	1263
BsrI	ACTGG	3 cut(s)	902, 1006, 1206
BssAI	RCCGGY	1 cut(s)	1263
BssECI	CCNNGG	4 cut(s)	78, 108, 277, 1042
BssT1I	CCWWGG	2 cut(s)	108, 1042
Bst2UI	CCWGG	2 cut(s)	392, 1440
Bst4CI	ACNGT	5 cut(s)	281, 797, 826, 1529, 1559
Bst6I	CTCTTC	1 cut(s)	140
BstAPI	GCANNNNNTGC	1 cut(s)	1139
BstC8I	GCNNGC	6 cut(s)	57, 618, 657, 970, 1349, 1626
BstDEI	CTNAG	5 cut(s)	145, 342, 843, 1190, 1478
BstDSI	CCRYGG	2 cut(s)	78, 277
BstENI	CCTNNNNNAGG	2 cut(s)	473, 1326
BstF5I	GGATG	4 cut(s)	508, 1151, 1218, 1546
BstFNI	CGCG	2 cut(s)	59, 117
BstHHI	GCGC	3 cut(s)	61, 1125, 1765
BstMAI	GTCTC	2 cut(s)	864, 891
BstMCI	CGRYCG	1 cut(s)	1345
BstMWI	GCNNNNNNNGC	4 cut(s)	83, 464, 1139, 1283
BstNI	CCWGG	2 cut(s)	392, 1440
BstNSI	RCATGY	2 cut(s)	432, 789
BstSCI	CCNGG	2 cut(s)	390, 1438
BstSFI	CTRYAG	3 cut(s)	97, 639, 1411
BstSLI	GKGCMC	2 cut(s)	618, 998
BstUI	CGCG	2 cut(s)	59, 117
BstV1I	GCAGC	5 cut(s)	83, 111, 521, 1142, 1428
BstX2I	RGATCY	2 cut(s)	916, 1012
BstXI	CCANNNNNNTGG	1 cut(s)	1213
BstYI	RGATCY	2 cut(s)	916, 1012
BsuRI	GGCC	2 cut(s)	467, 1779
BtgI	CCRYGG	2 cut(s)	78, 277
BtsCI	GGATG	4 cut(s)	508, 1151, 1218, 1546
BtsI	GCAGTG	1 cut(s)	1680
BtsIMutI	CAGTG	1 cut(s)	1680
Cac8I	GCNNGC	6 cut(s)	57, 618, 657, 970, 1349, 1626
CaiI	CAGNNNCTG	1 cut(s)	801
CciI	TCATGA	3 cut(s)	334, 790, 808
CfoI	GCGC	3 cut(s)	61, 1125, 1765
Cfr10I	RCCGGY	1 cut(s)	1263
Cfr13I	GGNCC	6 cut(s)	112, 388, 466, 1217, 1266, 1455
CseI	GACGC	1 cut(s)	106
Csp6I	GTAC	1 cut(s)	293
CspAI	ACCGGT	1 cut(s)	1263
CviQI	GTAC	1 cut(s)	293
DdeI	CTNAG	5 cut(s)	145, 342, 843, 1190, 1478
DraI	TTTAAA	1 cut(s)	1257
DrdI	GACNNNNNNGTC	1 cut(s)	607
DseDI	GACNNNNNNGTC	1 cut(s)	607
Eam1104I	CTCTTC	1 cut(s)	140
EarI	CTCTTC	1 cut(s)	140
EciI	GGCGGA	3 cut(s)	87, 1553, 1653
Eco130I	CCWWGG	2 cut(s)	108, 1042
Eco147I	AGGCCT	1 cut(s)	1779
Eco24I	GRGCYC	1 cut(s)	1367
Eco31I	GGTCTC	1 cut(s)	891
Eco47I	GGWCC	5 cut(s)	112, 388, 1217, 1266, 1455
Eco57I	CTGAAG	1 cut(s)	747
Eco88I	CYCGRG	2 cut(s)	170, 1008
EcoNI	CCTNNNNNAGG	2 cut(s)	473, 1326
EcoO109I	RGGNCCY	1 cut(s)	388
EcoRII	CCWGG	2 cut(s)	390, 1438
EcoT14I	CCWWGG	2 cut(s)	108, 1042
EcoT22I	ATGCAT	1 cut(s)	1569
EcoT38I	GRGCYC	1 cut(s)	1367
ErhI	CCWWGG	2 cut(s)	108, 1042
FaqI	GGGAC	1 cut(s)	560
FauI	CCCGC	2 cut(s)	60, 759
FauNDI	CATATG	2 cut(s)	622, 664
FbaI	TGATCA	2 cut(s)	270, 1578
Fnu4HI	GCNGC	8 cut(s)	84, 87, 97, 100, 535, 1131, 1417, 1491
FokI	GGATG	4 cut(s)	495, 1158, 1225, 1553
FriOI	GRGCYC	1 cut(s)	1367
Fsp4HI	GCNGC	8 cut(s)	84, 87, 97, 100, 535, 1131, 1417, 1491
FspBI	CTAG	2 cut(s)	312, 470
GlaI	GCGC	3 cut(s)	60, 1124, 1764
GluI	GCNGC	8 cut(s)	84, 87, 97, 100, 535, 1131, 1417, 1491
GsaI	CCCAGC	1 cut(s)	1624
HaeIII	GGCC	2 cut(s)	467, 1779
HapII	CCGG	3 cut(s)	1143, 1264, 1704
HgaI	GACGC	1 cut(s)	106
HhaI	GCGC	3 cut(s)	61, 1125, 1765
Hin6I	GCGC	3 cut(s)	59, 1123, 1763
HinP1I	GCGC	3 cut(s)	59, 1123, 1763
HincII	GTYRAC	1 cut(s)	1399
HindII	GTYRAC	1 cut(s)	1399
HinfI	GANTC	6 cut(s)	38, 191, 338, 369, 490, 548
HpaI	GTTAAC	1 cut(s)	1399
HpaII	CCGG	3 cut(s)	1143, 1264, 1704
HphI	GGTGA	3 cut(s)	97, 559, 701
Hpy166II	GTNNAC	3 cut(s)	996, 1399, 1695
Hpy188III	TCNNGA	7 cut(s)	335, 791, 809, 894, 920, 1010, 1322
Hpy8I	GTNNAC	3 cut(s)	996, 1399, 1695
Hpy99I	CGWCG	1 cut(s)	122
HpyAV	CCTTC	9 cut(s)	140, 386, 517, 771, 786, 940, 1301, 1627, 1657
HpyCH4III	ACNGT	5 cut(s)	281, 797, 826, 1529, 1559
HpyCH4IV	ACGT	3 cut(s)	7, 404, 1691
HpyF10VI	GCNNNNNNNGC	4 cut(s)	83, 464, 1139, 1283
HpyF3I	CTNAG	5 cut(s)	145, 342, 843, 1190, 1478
HpySE526I	ACGT	3 cut(s)	7, 404, 1691
HspAI	GCGC	3 cut(s)	59, 1123, 1763
Ksp22I	TGATCA	2 cut(s)	270, 1578
KspAI	GTTAAC	1 cut(s)	1399
LmnI	GCTCC	4 cut(s)	74, 562, 748, 1362
Lsp1109I	GCAGC	5 cut(s)	83, 111, 521, 1142, 1428
LweI	GCATC	8 cut(s)	216, 308, 644, 970, 1142, 1531, 1576, 1621
MaeI	CTAG	2 cut(s)	312, 470
MaeII	ACGT	3 cut(s)	7, 404, 1691
MaeIII	GTNAC	2 cut(s)	598, 644
MbiI	CCGCTC	1 cut(s)	1341
MboII	GAAGA	8 cut(s)	127, 327, 691, 720, 764, 831, 926, 1692
MflI	RGATCY	2 cut(s)	916, 1012
MhlI	GDGCHC	5 cut(s)	618, 998, 1009, 1367, 1522
MluCI	AATT	7 cut(s)	242, 540, 957, 1317, 1497, 1723, 1754
MlyI	GAGTC	3 cut(s)	185, 363, 542
MmeI	TCCRAC	1 cut(s)	1190
MnlI	CCTC	8 cut(s)	57, 113, 722, 873, 878, 1267, 1322, 1769
Mph1103I	ATGCAT	1 cut(s)	1569
MroXI	GAANNNNTTC	3 cut(s)	129, 838, 1740
MseI	TTAA	4 cut(s)	140, 453, 1256, 1398
MslI	CAYNNNNRTG	2 cut(s)	621, 989
MspI	CCGG	3 cut(s)	1143, 1264, 1704
MspR9I	CCNGG	2 cut(s)	392, 1440
MvaI	CCWGG	2 cut(s)	392, 1440
MvnI	CGCG	2 cut(s)	59, 117
MwoI	GCNNNNNNNGC	4 cut(s)	83, 464, 1139, 1283
NdeI	CATATG	2 cut(s)	622, 664
NlaIV	GGNNCC	6 cut(s)	76, 389, 558, 1218, 1364, 1489
NmuCI	GTSAC	1 cut(s)	598
NsiI	ATGCAT	1 cut(s)	1569
NspI	RCATGY	2 cut(s)	432, 789
PaeR7I	CTCGAG	2 cut(s)	170, 1008
PagI	TCATGA	3 cut(s)	334, 790, 808
PceI	AGGCCT	1 cut(s)	1779
PciI	ACATGT	2 cut(s)	428, 785
PdmI	GAANNNNTTC	3 cut(s)	129, 838, 1740
PfeI	GAWTC	3 cut(s)	38, 338, 490
PflFI	GACNNNGTC	1 cut(s)	116
PflMI	CCANNNNNTGG	1 cut(s)	518
PfoI	TCCNGGA	1 cut(s)	1438
PinAI	ACCGGT	1 cut(s)	1263
PkrI	GCNGC	8 cut(s)	85, 88, 98, 101, 536, 1132, 1418, 1492
PleI	GAGTC	3 cut(s)	185, 363, 542
PpsI	GAGTC	3 cut(s)	185, 363, 542
PpuMI	RGGWCCY	1 cut(s)	388
PscI	ACATGT	2 cut(s)	428, 785
PshBI	ATTAAT	1 cut(s)	453
Psp5II	RGGWCCY	1 cut(s)	388
Psp6I	CCWGG	2 cut(s)	390, 1438
PspFI	CCCAGC	1 cut(s)	1620
PspGI	CCWGG	2 cut(s)	390, 1438
PspN4I	GGNNCC	6 cut(s)	76, 389, 558, 1218, 1364, 1489
PspPI	GGNCC	6 cut(s)	112, 388, 466, 1217, 1266, 1455
PspPPI	RGGWCCY	1 cut(s)	388
PspXI	VCTCGAGB	1 cut(s)	170
PstI	CTGCAG	1 cut(s)	101
PstNI	CAGNNNCTG	1 cut(s)	801
PsuI	RGATCY	2 cut(s)	916, 1012
PsyI	GACNNNGTC	1 cut(s)	116
RsaI	GTAC	1 cut(s)	294
RsaNI	GTAC	1 cut(s)	293
RseI	CAYNNNNRTG	2 cut(s)	621, 989
SaqAI	TTAA	4 cut(s)	140, 453, 1256, 1398
SatI	GCNGC	8 cut(s)	84, 87, 97, 100, 535, 1131, 1417, 1491
Sau96I	GGNCC	6 cut(s)	112, 388, 466, 1217, 1266, 1455
SchI	GAGTC	3 cut(s)	185, 363, 542
ScrFI	CCNGG	2 cut(s)	392, 1440
SduI	GDGCHC	5 cut(s)	618, 998, 1009, 1367, 1522
SfaNI	GCATC	8 cut(s)	216, 308, 644, 970, 1142, 1531, 1576, 1621
SfcI	CTRYAG	3 cut(s)	97, 639, 1411
Sfr274I	CTCGAG	2 cut(s)	170, 1008
SinI	GGWCC	5 cut(s)	112, 388, 1217, 1266, 1455
SlaI	CTCGAG	2 cut(s)	170, 1008
SmiMI	CAYNNNNRTG	2 cut(s)	621, 989
SmlI	CTYRAG	2 cut(s)	170, 1008
SmoI	CTYRAG	2 cut(s)	170, 1008
Sse9I	AATT	7 cut(s)	242, 540, 957, 1317, 1497, 1723, 1754
SseBI	AGGCCT	1 cut(s)	1779
SspMI	CTAG	2 cut(s)	312, 470
StuI	AGGCCT	1 cut(s)	1779
StyD4I	CCNGG	2 cut(s)	390, 1438
StyI	CCWWGG	2 cut(s)	108, 1042
TaaI	ACNGT	5 cut(s)	281, 797, 826, 1529, 1559
TaiI	ACGT	3 cut(s)	10, 407, 1694
TaqI	TCGA	5 cut(s)	171, 444, 1009, 1650, 1774
TasI	AATT	7 cut(s)	242, 540, 957, 1317, 1497, 1723, 1754
TauI	GCSGC	3 cut(s)	86, 89, 1493
TfiI	GAWTC	3 cut(s)	38, 338, 490
Tru1I	TTAA	4 cut(s)	140, 453, 1256, 1398
Tru9I	TTAA	4 cut(s)	140, 453, 1256, 1398
TscAI	CASTG	1 cut(s)	1687
TseFI	GTSAC	1 cut(s)	598
TseI	GCWGC	5 cut(s)	96, 99, 534, 1130, 1416
Tsp45I	GTSAC	1 cut(s)	598
TspGWI	ACGGA	2 cut(s)	250, 684
TspRI	CASTG	1 cut(s)	1687
Tth111I	GACNNNGTC	1 cut(s)	116
Van91I	CCANNNNNTGG	1 cut(s)	518
VneI	GTGCAC	1 cut(s)	994
VpaK11BI	GGWCC	5 cut(s)	112, 388, 1217, 1266, 1455
VspI	ATTAAT	1 cut(s)	453
XagI	CCTNNNNNAGG	2 cut(s)	473, 1326
XapI	RAATTY	3 cut(s)	242, 540, 1723
XceI	RCATGY	2 cut(s)	432, 789
XhoI	CTCGAG	2 cut(s)	170, 1008
XmnI	GAANNNNTTC	3 cut(s)	129, 838, 1740
XspI	CTAG	2 cut(s)	312, 470
Zsp2I	ATGCAT	1 cut(s)	1569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.