RchiOBHm_Chr1g0337211

Ceramide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005461
Physical Location & Seq
Reverse (-)
28892377 .. 28896406
4030 bp
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UTR
Exon/CDS
Intron
PRQ56512

Sequence Viewer

Length: 594 bp
ATGTCTCGAATGCTCGAAGATATTTACCGCTTTACGGTGCATGGTTTCCAGAGCTCAAGGACTTTGCCGTCTGTTCCGGTCCTTGCTACATATAGATTTGGTCATAAGGATCTCCACATATGTCAGATATGGGTCAATCAAATCAATGCTTCTCTGGACTTAGAGCAGGGGATGCCAAAGAATCTTTTGGGAAATGCCTGCAAAACTTGGGACAGTGTGGCTCCTATATTCTCCCGTGCTAAAGTGAAAACAAAGGTGATTGTGACCGAAAGGGCAGTGCATGCATATGATGCGATGGCAACTATAGTAAACAGGGAGCTTATTTCATATGATGAAGTTATGGCTGTTGGTGGTGATGGCTTTTTCAACCAAATTCTTAATGGATTTCTTTCTTCAAGGCATAAAGCTCCATATCCACCAACTCCTTCAGATTTCCTTGATTCTGCTAGTTGTAATGAGAGTCTCGTTGTAAATGATCCAAGTGAAACCGTTATTGAAGCTTATTCTCAGAATAATGAGCAGTCTCCCCTCCTCTCAAGTTCAAATATTAATGGCTCAGGACTCAGGAGTCTCAAATATCAATCAAGAGCCTGA

Protein Analysis

197

Amino Acids

21.87

Weight (kDa)

6.51

Isoelectric Point (pI)

62.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 64 - 136 1.8e-09 Diacylglycerol kinase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 67, 567
AciI CCGC 1 cut(s) 28
AclWI GGATC 2 cut(s) 117, 470
AcsI RAATTY 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 4 cut(s) 367, 396, 497, 543
AluBI AGCT 4 cut(s) 54, 319, 407, 500
AluI AGCT 4 cut(s) 54, 319, 407, 500
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 4 cut(s) 9, 467, 528, 575
AlwI GGATC 2 cut(s) 117, 470
ApoI RAATTY 1 cut(s) 372
AseI ATTAAT 1 cut(s) 549
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 268, 365
AvaII GGWCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 56
BccI CCATC 2 cut(s) 289, 350
BceAI ACGGC 1 cut(s) 52
BcoDI GTCTC 4 cut(s) 9, 467, 528, 575
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 303
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 222
BmsI GCATC 2 cut(s) 162, 280
Bpu10I CCTNAGC 1 cut(s) 556
BpuEI CTTGAG 2 cut(s) 40, 520
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 1 cut(s) 34
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 3 cut(s) 521, 570, 577
BseRI GAGGAG 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 4 cut(s) 9, 467, 528, 575
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 2 cut(s) 109, 475
BspACI CCGC 1 cut(s) 28
BspCNI CTCAG 3 cut(s) 520, 569, 576
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 2 cut(s) 117, 470
BssMI GATC 2 cut(s) 109, 475
Bst4CI ACNGT 3 cut(s) 37, 215, 490
BstAPI GCANNNNNTGC 3 cut(s) 172, 281, 290
BstC8I GCNNGC 2 cut(s) 199, 282
BstDEI CTNAG 4 cut(s) 160, 507, 556, 563
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 2 cut(s) 112, 478
BstMAI GTCTC 4 cut(s) 9, 467, 528, 575
BstMBI GATC 2 cut(s) 109, 475
BstMWI GCNNNNNNNGC 3 cut(s) 172, 281, 290
BstNSI RCATGY 1 cut(s) 284
BstSFI CTRYAG 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BtgZI GCGATG 1 cut(s) 308
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 2 cut(s) 220, 282
Cac8I GCNNGC 2 cut(s) 199, 282
Cfr13I GGNCC 1 cut(s) 79
CviAII CATG 2 cut(s) 41, 281
CviJI RGCY 9 cut(s) 54, 221, 319, 344, 360, 407, 500, 555, 590
CviKI_1 RGCY 9 cut(s) 54, 221, 319, 344, 360, 407, 500, 555, 590
DdeI CTNAG 4 cut(s) 160, 507, 556, 563
DpnI GATC 2 cut(s) 111, 477
DpnII GATC 2 cut(s) 109, 475
DrdI GACNNNNNNGTC 2 cut(s) 67, 567
DseDI GACNNNNNNGTC 2 cut(s) 67, 567
Ecl136II GAGCTC 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 56
FaeI CATG 2 cut(s) 44, 284
FaqI GGGAC 1 cut(s) 224
FatI CATG 2 cut(s) 40, 280
FauNDI CATATG 3 cut(s) 119, 286, 328
FokI GGATG 1 cut(s) 184
FriOI GRGCYC 1 cut(s) 56
FspBI CTAG 1 cut(s) 447
HapII CCGG 1 cut(s) 77
Hin1II CATG 2 cut(s) 44, 284
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 5 cut(s) 181, 440, 460, 561, 568
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 268, 365
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 3 cut(s) 126, 430, 510
Hpy188III TCNNGA 6 cut(s) 6, 49, 155, 558, 565, 585
Hpy8I GTNNAC 1 cut(s) 310
HpyAV CCTTC 1 cut(s) 435
HpyCH4III ACNGT 3 cut(s) 37, 215, 490
HpyCH4V TGCA 4 cut(s) 40, 201, 280, 284
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 281, 290
HpyF3I CTNAG 4 cut(s) 160, 507, 556, 563
Hsp92II CATG 2 cut(s) 44, 284
Kzo9I GATC 2 cut(s) 109, 475
LmnI GCTCC 3 cut(s) 226, 316, 412
LpnPI CCDG 8 cut(s) 62, 90, 140, 152, 211, 298, 543, 550
LweI GCATC 2 cut(s) 162, 280
MaeI CTAG 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 262
MalI GATC 2 cut(s) 111, 477
MboI GATC 2 cut(s) 109, 475
MboII GAAGA 2 cut(s) 29, 384
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 1 cut(s) 372
MlyI GAGTC 3 cut(s) 469, 555, 577
MnlI CCTC 2 cut(s) 539, 542
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 2 cut(s) 378, 549
MslI CAYNNNNRTG 1 cut(s) 285
MspI CCGG 1 cut(s) 77
Mva1269I GAATGC 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 172, 281, 290
NdeI CATATG 3 cut(s) 119, 286, 328
NdeII GATC 2 cut(s) 109, 475
NlaIII CATG 2 cut(s) 44, 284
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 284
PaeI GCATGC 1 cut(s) 284
PctI GAATGC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 181, 440
PleI GAGTC 3 cut(s) 468, 555, 576
PpsI GAGTC 3 cut(s) 468, 555, 576
PshBI ATTAAT 1 cut(s) 549
Psp124BI GAGCTC 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 79
PsuI RGATCY 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 285
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 2 cut(s) 378, 549
Sau3AI GATC 2 cut(s) 109, 475
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 3 cut(s) 469, 555, 577
SduI GDGCHC 1 cut(s) 56
SetI ASST 5 cut(s) 56, 258, 321, 409, 502
SfaNI GCATC 2 cut(s) 162, 280
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 55, 535
SmoI CTYRAG 2 cut(s) 55, 535
SphI GCATGC 1 cut(s) 284
Sse9I AATT 1 cut(s) 372
SsiI CCGC 1 cut(s) 28
SspI AATATT 1 cut(s) 547
SspMI CTAG 1 cut(s) 447
SstI GAGCTC 1 cut(s) 56
TaaI ACNGT 3 cut(s) 37, 215, 490
TaqI TCGA 2 cut(s) 7, 15
TaqII GACCGA 1 cut(s) 281
TasI AATT 1 cut(s) 372
TfiI GAWTC 2 cut(s) 181, 440
Tru1I TTAA 2 cut(s) 378, 549
Tru9I TTAA 2 cut(s) 378, 549
TscAI CASTG 2 cut(s) 220, 282
TseFI GTSAC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 315, 348
TspRI CASTG 2 cut(s) 220, 282
VpaK11BI GGWCC 1 cut(s) 79
VspI ATTAAT 1 cut(s) 549
XapI RAATTY 1 cut(s) 372
XceI RCATGY 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XspI CTAG 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.