MD01G1214500.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012779
Physical Location & Seq
Forward (+)
30790168 .. 30791244
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1214500.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGAAGTCTTTGGATGCGGAGTCGTGTATTTTGTGTCCAATCAAATACACGGAGCACAGAAGCAGGACAAGGAAAGTGACCAAACCGCGAAAGAAGTCGACAGCCGAGTTCAACTCGTCAGTCATCCCGAGAATCGTACGAATATCGGTGACCGATCCCGACGCCACGGATTCCTCCAGCGACGAAGGGGAGGAGTTTTTCGGGCGGCAGAGAGTGAAGCACTATATTAACGAAGTCAACATCGAGAAGGACTTCAGGAACAGCCTTGTCATCAAGAACAACGCCCGGAAGCGGCCGGCTGCTGAGGTTCCGGCCAACCGGCGGCCAATGAAGAACGCAGCTTCGCCGACCAACGGCGGCAAGAAGTACCGCGGCGTAAGGCAACGGCCTTGGGGGAAGTGGGCGGCTGAGATTAGAGATCCTGCCAGACGTCAACGCCTGTGGCTGGGGACCTTCGACACGGCGGAGGAAGCCGCCATGGTGTACGATAATGCCGCTATTAAGCTCCGCGGTCCCGACGCTTTGACTAATTTCGTGGCTCCGCCGCCGAAAGAGGAGGAAAAGCCAGAGCCGCTGGTTAACGAGCCTGTCGAGGTGGAGACTAACGGAACATCGTCGGTTTCCGGTTACGATTCCGGGTCCGAGTCTCACAATCTGGCCTCTCCGACCTCCGTCCTCCATTTCAGAAGCCAACCCATCATTGAAGAAGTTGACGAGGCTCAAAGATTCCAGATGTTCGACCGAGTCGATGAGTATGAAGACGAAACCGGTACGAACATTTTGTCCGACGATTTGTCCGATTTCGCTAACTATTTGCCGCTGGACTTGCCTTACTTCGACGACGCATTCAGTTTCTCAGCTTCGGAGTCGCCGCTGTTCGACTCGCCTCTGTTTTTCGACGACGACTCCGCCGCAGCAGCCGATACTCTTCCGGATTGCTCTGTGAAGGACGATTTCAGCGAGTTGTTCCGGGACACGTTCGACGCGTCGCTGTCGCATACGGCGTCGTCTATGTGCCAAGGGGATGACTACTTTCAAGACATTTTGTTTGGTTCCGACCCGCTGGTGGTCCTCTGA

Protein Analysis

359

Amino Acids

40.18

Weight (kDa)

4.75

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 122 - 171 2e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27950 AT5G53290
fragaria_vesca FvH4_7g30930
malus_domestica MD01G1214500.v1.1 MD07G1284200.v1.1
prunus_persica Prupe.2G306400_v2.0.a1
pyrus_communis pycom01g22510 pycom07g25810
rosa_chinensis RchiOBHm_Chr1g0380021
rosa_laevigata RLG00000026340
rosa_multiflora Rmu_sc0004184.1_g000009
rosa_roxburghii Rroxscaffold_4G00279790
rosa_rugosa Rorug01G0416600
rosa_samantha Rh1AG441100 Rh1BG396800 Rh1CG410000 Rh1DG427600
rosa_wichuraiana Rw1G038360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 433
AccI GTMKAC 1 cut(s) 98
AccII CGCG 4 cut(s) 88, 372, 510, 986
AccIII TCCGGA 1 cut(s) 931
AclWI GGATC 2 cut(s) 149, 413
AcoI YGGCCR 3 cut(s) 293, 312, 323
AcuI CTGAAG 1 cut(s) 238
AcyI GRCGYC 3 cut(s) 162, 430, 1004
AfaI GTAC 4 cut(s) 138, 368, 485, 772
AfiI CCNNNNNNNGG 5 cut(s) 291, 321, 353, 445, 1066
AflIII ACRYGT 2 cut(s) 975, 984
AgeI ACCGGT 1 cut(s) 767
AgsI TTSAA 3 cut(s) 112, 704, 1037
AhdI GACNNNNNGTC 1 cut(s) 793
AloI GAACNNNNNNTCC 2 cut(s) 767, 799
AluBI AGCT 3 cut(s) 341, 505, 860
AluI AGCT 3 cut(s) 341, 505, 860
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 2 cut(s) 593, 651
AlwI GGATC 2 cut(s) 149, 413
Ama87I CYCGRG 1 cut(s) 127
Aor13HI TCCGGA 1 cut(s) 931
AoxI GGCC 5 cut(s) 293, 312, 323, 386, 657
ApeKI GCWGC 4 cut(s) 299, 338, 914, 917
ArsI GACNNNNNNTTYG 4 cut(s) 767, 799, 1031, 1063
AsiGI ACCGGT 1 cut(s) 767
Asp700I GAANNNNTTC 1 cut(s) 251
AspS9I GGNCC 4 cut(s) 450, 512, 639, 1069
AsuC2I CCSGG 3 cut(s) 286, 637, 971
AsuHPI GGTGA 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 4 cut(s) 450, 512, 639, 1069
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 57
BbvCI CCTCAGC 1 cut(s) 303
BbvI GCAGC 4 cut(s) 286, 350, 926, 929
BccI CCATC 1 cut(s) 704
BceAI ACGGC 4 cut(s) 370, 401, 477, 1017
BcnI CCSGG 3 cut(s) 286, 637, 971
BcoDI GTCTC 2 cut(s) 593, 651
Bme1390I CCNGG 3 cut(s) 286, 637, 971
Bme18I GGWCC 4 cut(s) 450, 512, 639, 1069
BmeRI GACNNNNNGTC 1 cut(s) 793
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 4 cut(s) 450, 512, 639, 1069
BmiI GGNNCC 6 cut(s) 309, 451, 514, 540, 640, 1054
BmrFI CCNGG 3 cut(s) 286, 637, 971
BmsI GCATC 1 cut(s) 4
BoxI GACNNNNGTC 1 cut(s) 671
BpiI GAAGAC 1 cut(s) 765
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 1 cut(s) 160
Bpu10I CCTNAGC 1 cut(s) 303
BpuMI CCSGG 3 cut(s) 286, 637, 971
BsaHI GRCGYC 3 cut(s) 162, 430, 1004
BsaJI CCNNGG 6 cut(s) 165, 370, 389, 477, 508, 1018
BsaWI WCCGGW 3 cut(s) 623, 767, 931
BsaXI ACNNNNNCTCC 2 cut(s) 890, 920
Bsc4I CCNNNNNNNGG 5 cut(s) 291, 321, 353, 445, 1066
Bse118I RCCGGY 3 cut(s) 295, 318, 767
BseAI TCCGGA 1 cut(s) 931
BseDI CCNNGG 6 cut(s) 165, 370, 389, 477, 508, 1018
BseGI GGATG 3 cut(s) 19, 123, 1030
BseLI CCNNNNNNNGG 5 cut(s) 291, 321, 353, 445, 1066
BseMII CTCAG 3 cut(s) 294, 399, 870
BseRI GAGGAG 2 cut(s) 206, 569
BseX3I CGGCCG 1 cut(s) 293
BseXI GCAGC 4 cut(s) 286, 350, 926, 929
BseYI CCCAGC 1 cut(s) 445
Bsh1236I CGCG 4 cut(s) 88, 372, 510, 986
Bsh1285I CGRYCG 2 cut(s) 296, 742
BshFI GGCC 5 cut(s) 295, 314, 325, 388, 659
BshTI ACCGGT 1 cut(s) 767
BsiEI CGRYCG 2 cut(s) 296, 742
BsiHKAI GWGCWC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 9 cut(s) 286, 296, 311, 319, 624, 636, 768, 932, 970
BsiWI CGTACG 1 cut(s) 136
BslFI GGGAC 3 cut(s) 463, 498, 986
BslI CCNNNNNNNGG 5 cut(s) 291, 321, 353, 445, 1066
BsmAI GTCTC 2 cut(s) 593, 651
BsmFI GGGAC 3 cut(s) 463, 498, 986
BsmI GAATGC 1 cut(s) 845
BsnI GGCC 5 cut(s) 295, 314, 325, 388, 659
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 57
Bsp13I TCCGGA 1 cut(s) 931
Bsp143I GATC 2 cut(s) 154, 418
Bsp19I CCATGG 1 cut(s) 477
BspANI GGCC 5 cut(s) 295, 314, 325, 388, 659
BspCNI CTCAG 3 cut(s) 295, 400, 869
BspEI TCCGGA 1 cut(s) 931
BspFNI CGCG 4 cut(s) 88, 372, 510, 986
BspLI GGNNCC 6 cut(s) 309, 451, 514, 540, 640, 1054
BspPI GGATC 2 cut(s) 149, 413
BsrFI RCCGGY 3 cut(s) 295, 318, 767
BssAI RCCGGY 3 cut(s) 295, 318, 767
BssECI CCNNGG 6 cut(s) 165, 370, 389, 477, 508, 1018
BssMI GATC 2 cut(s) 154, 418
BssNI GRCGYC 3 cut(s) 162, 430, 1004
BssT1I CCWWGG 3 cut(s) 389, 477, 1018
Bst6I CTCTTC 1 cut(s) 933
BstACI GRCGYC 3 cut(s) 162, 430, 1004
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 3 cut(s) 303, 408, 856
BstDSI CCRYGG 4 cut(s) 165, 370, 477, 508
BstEII GGTNACC 1 cut(s) 148
BstF5I GGATG 3 cut(s) 19, 123, 1030
BstFNI CGCG 4 cut(s) 88, 372, 510, 986
BstKTI GATC 2 cut(s) 157, 421
BstMAI GTCTC 2 cut(s) 593, 651
BstMBI GATC 2 cut(s) 154, 418
BstMCI CGRYCG 2 cut(s) 296, 742
BstMWI GCNNNNNNNGC 4 cut(s) 470, 571, 826, 917
BstPAI GACNNNNGTC 1 cut(s) 671
BstPI GGTNACC 1 cut(s) 148
BstSCI CCNGG 3 cut(s) 284, 635, 969
BstUI CGCG 4 cut(s) 88, 372, 510, 986
BstV1I GCAGC 4 cut(s) 286, 350, 926, 929
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BstZI CGGCCG 1 cut(s) 293
BsuRI GGCC 5 cut(s) 295, 314, 325, 388, 659
BtgI CCRYGG 4 cut(s) 165, 370, 477, 508
BtsCI GGATG 3 cut(s) 19, 123, 1030
Cac8I GCNNGC 1 cut(s) 297
Cfr10I RCCGGY 3 cut(s) 295, 318, 767
Cfr13I GGNCC 4 cut(s) 450, 512, 639, 1069
Cfr42I CCGCGG 2 cut(s) 373, 511
CseI GACGC 6 cut(s) 170, 527, 851, 975, 992, 993
Csp6I GTAC 4 cut(s) 137, 367, 484, 771
CspAI ACCGGT 1 cut(s) 767
CspCI CAANNNNNGTGG 2 cut(s) 422, 457
CviAII CATG 1 cut(s) 478
CviQI GTAC 4 cut(s) 137, 367, 484, 771
DdeI CTNAG 3 cut(s) 303, 408, 856
DpnI GATC 2 cut(s) 156, 420
DpnII GATC 2 cut(s) 154, 418
DriI GACNNNNNGTC 1 cut(s) 793
EaeI YGGCCR 3 cut(s) 293, 312, 323
EagI CGGCCG 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 933
Eam1105I GACNNNNNGTC 1 cut(s) 793
EarI CTCTTC 1 cut(s) 933
EciI GGCGGA 3 cut(s) 479, 531, 898
EclXI CGGCCG 1 cut(s) 293
Eco130I CCWWGG 3 cut(s) 389, 477, 1018
Eco47I GGWCC 4 cut(s) 450, 512, 639, 1069
Eco52I CGGCCG 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 127
Eco91I GGTNACC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 148
EcoT14I CCWWGG 3 cut(s) 389, 477, 1018
ErhI CCWWGG 3 cut(s) 389, 477, 1018
FaeI CATG 1 cut(s) 481
FaiI YATR 5 cut(s) 225, 479, 756, 999, 1013
FaqI GGGAC 3 cut(s) 463, 498, 986
FatI CATG 1 cut(s) 477
FauI CCCGC 1 cut(s) 1068
FblI GTMKAC 1 cut(s) 98
FokI GGATG 3 cut(s) 26, 110, 1037
GsaI CCCAGC 1 cut(s) 449
GsuI CTGGAG 1 cut(s) 160
HaeIII GGCC 5 cut(s) 295, 314, 325, 388, 659
HapII CCGG 9 cut(s) 286, 296, 311, 319, 624, 636, 768, 932, 970
HgaI GACGC 6 cut(s) 170, 527, 851, 975, 992, 993
Hin1I GRCGYC 3 cut(s) 162, 430, 1004
Hin1II CATG 1 cut(s) 481
HincII GTYRAC 5 cut(s) 99, 238, 434, 580, 712
HindII GTYRAC 5 cut(s) 99, 238, 434, 580, 712
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 9 cut(s) 286, 296, 311, 319, 624, 636, 768, 932, 970
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 6 cut(s) 99, 238, 434, 484, 580, 712
Hpy188I TCNGA 8 cut(s) 643, 666, 686, 787, 799, 865, 1057, 1076
Hpy188III TCNNGA 9 cut(s) 127, 158, 244, 256, 274, 515, 730, 932, 1037
Hpy8I GTNNAC 6 cut(s) 99, 238, 434, 484, 580, 712
HpyAV CCTTC 4 cut(s) 179, 241, 463, 940
HpyCH4IV ACGT 2 cut(s) 430, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 470, 571, 826, 917
HpyF3I CTNAG 3 cut(s) 303, 408, 856
HpySE526I ACGT 2 cut(s) 430, 977
Hsp92I GRCGYC 3 cut(s) 162, 430, 1004
Hsp92II CATG 1 cut(s) 481
Kpn2I TCCGGA 1 cut(s) 931
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 580
KspI CCGCGG 2 cut(s) 373, 511
Kzo9I GATC 2 cut(s) 154, 418
LmnI GCTCC 3 cut(s) 52, 510, 544
Lsp1109I GCAGC 4 cut(s) 286, 350, 926, 929
LweI GCATC 1 cut(s) 4
MaeII ACGT 2 cut(s) 430, 977
MaeIII GTNAC 3 cut(s) 76, 148, 626
MalI GATC 2 cut(s) 156, 420
MboI GATC 2 cut(s) 154, 418
MboII GAAGA 4 cut(s) 343, 716, 770, 920
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 1 cut(s) 529
MluI ACGCGT 1 cut(s) 984
MlyI GAGTC 6 cut(s) 29, 653, 753, 875, 875, 899
MmeI TCCRAC 2 cut(s) 689, 810
MroI TCCGGA 1 cut(s) 931
MroNI GCCGGC 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 3 cut(s) 228, 501, 579
MspA1I CMGCKG 6 cut(s) 372, 510, 574, 820, 874, 1063
MspI CCGG 9 cut(s) 286, 296, 311, 319, 624, 636, 768, 932, 970
MspR9I CCNGG 3 cut(s) 286, 637, 971
Mva1269I GAATGC 1 cut(s) 845
MvnI CGCG 4 cut(s) 88, 372, 510, 986
MwoI GCNNNNNNNGC 4 cut(s) 470, 571, 826, 917
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 3 cut(s) 286, 637, 971
NcoI CCATGG 1 cut(s) 477
NdeII GATC 2 cut(s) 154, 418
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 6 cut(s) 309, 451, 514, 540, 640, 1054
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 76, 148
PcsI WCGNNNNNNNCGW 6 cut(s) 588, 744, 795, 957, 983, 1001
PctI GAATGC 1 cut(s) 845
PdiI GCCGGC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 4 cut(s) 132, 170, 632, 726
Pfl23II CGTACG 1 cut(s) 136
PflFI GACNNNGTC 1 cut(s) 743
PfoI TCCNGGA 1 cut(s) 969
PinAI ACCGGT 1 cut(s) 767
PleI GAGTC 6 cut(s) 28, 652, 752, 874, 875, 899
PpsI GAGTC 6 cut(s) 28, 652, 752, 874, 875, 899
PpuMI RGGWCCY 1 cut(s) 450
PshAI GACNNNNGTC 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 450
PspEI GGTNACC 1 cut(s) 148
PspFI CCCAGC 1 cut(s) 445
PspLI CGTACG 1 cut(s) 136
PspN4I GGNNCC 6 cut(s) 309, 451, 514, 540, 640, 1054
PspPI GGNCC 4 cut(s) 450, 512, 639, 1069
PspPPI RGGWCCY 1 cut(s) 450
PsuI RGATCY 1 cut(s) 418
PsyI GACNNNGTC 1 cut(s) 743
RsaI GTAC 4 cut(s) 138, 368, 485, 772
RsaNI GTAC 4 cut(s) 137, 367, 484, 771
SacII CCGCGG 2 cut(s) 373, 511
SalI GTCGAC 1 cut(s) 97
SaqAI TTAA 3 cut(s) 228, 501, 579
Sau3AI GATC 2 cut(s) 154, 418
Sau96I GGNCC 4 cut(s) 450, 512, 639, 1069
SchI GAGTC 6 cut(s) 29, 653, 753, 875, 875, 899
ScrFI CCNGG 3 cut(s) 286, 637, 971
SduI GDGCHC 1 cut(s) 57
SetI ASST 9 cut(s) 309, 343, 433, 455, 507, 597, 671, 862, 980
SfaNI GCATC 1 cut(s) 4
Sfr303I CCGCGG 2 cut(s) 373, 511
SgrBI CCGCGG 2 cut(s) 373, 511
SinI GGWCC 4 cut(s) 450, 512, 639, 1069
Sse9I AATT 1 cut(s) 529
StyD4I CCNGG 3 cut(s) 284, 635, 969
StyI CCWWGG 3 cut(s) 389, 477, 1018
TaiI ACGT 2 cut(s) 433, 980
TaqII GACCGA 2 cut(s) 167, 756
TasI AATT 1 cut(s) 529
TfiI GAWTC 4 cut(s) 132, 170, 632, 726
Tru1I TTAA 3 cut(s) 228, 501, 579
Tru9I TTAA 3 cut(s) 228, 501, 579
TseFI GTSAC 2 cut(s) 76, 148
TseI GCWGC 4 cut(s) 299, 338, 914, 917
Tsp45I GTSAC 2 cut(s) 76, 148
TspDTI ATGAA 3 cut(s) 17, 344, 771
TspGWI ACGGA 4 cut(s) 65, 182, 621, 661
Tth111I GACNNNGTC 1 cut(s) 743
VpaK11BI GGWCC 4 cut(s) 450, 512, 639, 1069
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 251
ZraI GACGTC 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.