Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005221

Physical Location & Seq

Genomic Coordinates Forward (+)

31009834 .. 31013389

Gene Structure 3556 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1217300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGAATTTTCAGCAAGAGAAGTTCGTCAGGTTTGAGGATTGGACTGCAGAGAAAAATGTTGAGCCATTATATTCTCCAGATGATGACATGCCACCAGGGAAGTTCAGAAGAACGATTAACTCGGTGTCAATCAAATTCCAAAGAAGCTTGGAATCTGGTTCTGAGCGGATTAAGAAGTCATGGAAATCTTATTCAATTGACAGTGTTGTTGGCAACAGCTTTGGTAGTGGAGTTCTTGATCCTCAAGGGCCATTCCTTCAGAAGTGGAATAAGATATTTGTATTGGCATGTATTATTGCCGTCTCCCTTGATCCCTTGTTCTTTTACATCCCTGTGATCAATGATAAGAGGAAGTGTCTTGGTTTGGACAGGAAGATGGAGATAACAGCTAGTGTTTTGCGTTCATTCACAGATATATTTTATATTGTTCACATAATTTTCCAGTTTCGTACTGGATTTATTGCTCCTTCTTCTCGAGTGTTTGGAAGAGGTGTTTTAGTTGAAGATGCTTGGGCTATTGCAAGGAGGTATCTCTCATCATACTTCCTAATTGACATTCTTGCAGTCCTTCCTCTCCCACAAGTGGTTATTCTAATTTTCATTCCAAAATTGGGAGGCTCAAAAACGTTGAATACAAAGAATTTGTTGAAGTTTGTTGTTCTGTTCCAGTATTTTCCAAGGTTTATACGGATATATCCATTGTATAGAGAAGTCACAAGGGCTTCTGGTATACTTACCGAAACTGCTTGGGCAGGAGCTGCATTTAATCTTTTTCTTTACATGCTCGCCAGTCATGTACTGGGAGCTTTTTGGTACTTGTTCTCTATAGAACGTGAAACCAAGTGCTGGAAAGCAGCATGTGGGAACAATACAACCATATGCTCGCACAAGGATTTATACTGTGATACCGTGAGCTCGAATGCTTTTAGTAAGATCACATTCTTAAATTCTTCCTGCCCGATACAAGATGAAGATAAAACACAGTTTGATTTTGGAATATTCCTTGATGCCCTTCAATCTGGTATTGTTGAGTCAAGCACAGATTTTCCTCAGAAATTCTTTTACTGCTTTTGGTGGGGCCTACGGAATTTGAGTTCACTTGGTCAGAACCTTGCAACCAGTACATATGTATGGGAAATCTGCTTTGCAGTCTTCATTTCTATCGCTGGGTTGGTGCTATTTTCATTTCTCATCGGAAATATGCAGACATATTTGCAATCTACAACCACACGATTGGAGGAAATGAGAGTGAAAAGGAGAGATGCAGAACAATGGATGTCCCACCGCTTGCTACCTGAGAATTTGCGAGAGCGCATCAGGCGGTATGAACAATATAAATGGCAAGAAACCAGAGGTGTTGATGAAGAGAATTTGATATGTAATCTTCCCAAGGATCTTAGAAGGGACATAAAGCGCCATCTCTGTTTGGCTCTGCTTATGAGAGTGCCAATGTTCGAGAAAATGGATGAACAACTGCTCGATGCAATGTGTGACCGTCTCAAGCCGGTACTATATACAGAAGAAAGCTATATCGTTAGGGAGGGAGACCCAGTGGATGAGATGCTCTTTATCATGCGGGGCAGACTATTGACTATGACCACGAATGGTGGAAGAACCGGTTTCTTCAACTCTGAATATCTCAAGGCCGGTGACTTTTGCGGCGAAGAACTTTTGACATGGGCTCTTGATCCCCACACATCAGCTAATCTTCCCATCTCGACCAGAACTGTCCAAGCCCTCAGTGAAGTTGAAGCCTTTGCCTTAAAAGCGGATGACTTGAAGTTTGTAGCCTCTCAGTTCAGGCGACTCCACAGCAAGCAGCTCCGGCACACATTCAGGTTCTACTCACAGCAGTGGAGAACTTGGGCAGCTACTTTTATACAAGCAGCGTGGCGCCGTCATATGAAGAAGAAGCTAGAAGAGTCTCTGCAGGAAGAGGAGAATAGGCTGCAAGATGCACTGGCCAAGGCTGGGGTCAGCTCCCCAAGTCTAGGGGCCACCATTTATGCCTCACGTTTTGCTGCTAATATACTGCGTACTATACGGCGTAGTGGTACGCGGAAGGCGAGGGTGCCAGAGAGACTACCGGCCATGCTACTTCAGAAGCCGGCGGAGCCTGATTTTACTGCTGAAGAGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0008150 GO:0016020 GO:0016021 GO:0031224 GO:0031226 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0065007 GO:0065008 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

714

Amino Acids

82.48

Weight (kDa)

9.05

Isoelectric Point (pI)

51.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	91 - 411	5.6e-17	Ion transport protein
cNMP_binding	PF00027	505 - 596	6.9e-07	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53130
fragaria_vesca	FvH4_2g31110 FvH4_7g31200 FvH4_7g31200 FvH4_7g31200 FvH4_7g31200
malus_domestica	MD01G1217100.v1.1 MD01G1217300.v1.1 MD07G1286700.v1.1
prunus_persica	Prupe.2G308700_v2.0.a1 Prupe.2G308700_v2.0.a1 Prupe.2G308700_v2.0.a1
pyrus_communis	pycom01g22750 pycom07g26000
rosa_chinensis	RchiOBHm_Chr1g0380261 RchiOBHm_Chr1g0380301
rosa_laevigata	RLG00000026314
rosa_multiflora	Rmu_sc0001232.1_g000031 Rmu_sc0002387.1_g000001 Rmu_sc0002387.1_g000009 Rmu_sc0003249.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279530
rosa_rugosa	Rorug01G0419600 Rorug01G0419700
rosa_samantha	Rh1AG444200 Rh1AG444300 Rh1CG412900
rosa_wichuraiana	Rw1G038580 Rw1G038650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1893, 2071
AccB7I	CCANNNNNTGG	1 cut(s)	846
AccBSI	CCGCTC	1 cut(s)	166
AccI	GTMKAC	1 cut(s)	730
AccII	CGCG	1 cut(s)	2059
AciI	CCGC	8 cut(s)	166, 1285, 1321, 1576, 1659, 1769, 2059, 2111
AclI	AACGTT	1 cut(s)	626
AclWI	GGATC	4 cut(s)	233, 305, 1401, 1682
AcoI	YGGCCR	2 cut(s)	1962, 2088
AcsI	RAATTY	8 cut(s)	4, 134, 640, 946, 1055, 1087, 1300, 1369
AcuI	CTGAAG	2 cut(s)	242, 2084
AcyI	GRCGYC	1 cut(s)	1894
AfaI	GTAC	7 cut(s)	451, 798, 815, 1123, 1509, 2038, 2056
AfiI	CCNNNNNNNGG	5 cut(s)	583, 611, 846, 1971, 1991
AgeI	ACCGGT	1 cut(s)	1616
AgsI	TTSAA	8 cut(s)	195, 503, 631, 649, 1016, 1627, 1751, 1780
AjnI	CCWGG	1 cut(s)	94
AleI	CACNNNNGTG	1 cut(s)	1852
AloI	GAACNNNNNNTCC	2 cut(s)	1078, 1110
Alw21I	GWGCWC	1 cut(s)	917
Alw26I	GTCTC	5 cut(s)	307, 1502, 1539, 1929, 2074
AlwI	GGATC	4 cut(s)	233, 305, 1401, 1682
AlwNI	CAGNNNCTG	1 cut(s)	758
Ama87I	CYCGRG	1 cut(s)	474
AoxI	GGCC	6 cut(s)	248, 1078, 1644, 1962, 1995, 2088
ApeKI	GCWGC	7 cut(s)	758, 854, 1819, 1868, 1886, 1948, 2021
ApoI	RAATTY	8 cut(s)	4, 134, 640, 946, 1055, 1087, 1300, 1369
ArsI	GACNNNNNNTTYG	2 cut(s)	1766, 1798
AsiGI	ACCGGT	1 cut(s)	1616
AspLEI	GCGC	3 cut(s)	1314, 1416, 1896
AspS9I	GGNCC	3 cut(s)	248, 1078, 1995
AsuHPI	GGTGA	1 cut(s)	1661
AvaI	CYCGRG	1 cut(s)	474
BalI	TGGCCA	1 cut(s)	1964
BanI	GGYRCC	2 cut(s)	1893, 2071
BanII	GRGCYC	2 cut(s)	917, 1684
BbsI	GAAGAC	1 cut(s)	1144
Bbv12I	GWGCWC	1 cut(s)	917
BbvI	GCAGC	7 cut(s)	745, 866, 1831, 1880, 1898, 1935, 2008
BccI	CCATC	3 cut(s)	370, 1425, 1721
BceAI	ACGGC	3 cut(s)	284, 1881, 2060
BciT130I	CCWGG	1 cut(s)	96
BclI	TGATCA	1 cut(s)	336
BcoDI	GTCTC	5 cut(s)	307, 1502, 1539, 1929, 2074
BfaI	CTAG	3 cut(s)	390, 1916, 1991
BfmI	CTRYAG	3 cut(s)	45, 825, 1928
BfoI	RGCGCY	2 cut(s)	1417, 1897
BisI	GCNGC	8 cut(s)	759, 855, 1660, 1820, 1869, 1887, 1949, 2022
BlsI	GCNGC	8 cut(s)	760, 856, 1661, 1821, 1870, 1888, 1950, 2023
Bme1390I	CCNGG	1 cut(s)	96
BmeT110I	CYCGRG	1 cut(s)	474
BmgT120I	GGNCC	3 cut(s)	248, 1078, 1995
BmiI	GGNNCC	5 cut(s)	1079, 1895, 1996, 2073, 2115
BmrFI	CCNGG	1 cut(s)	96
BmrI	ACTGGG	2 cut(s)	809, 1544
BmsI	GCATC	7 cut(s)	496, 997, 1252, 1323, 1471, 1551, 1945
BmuI	ACTGGG	2 cut(s)	809, 1544
BpiI	GAAGAC	1 cut(s)	1144
BpmI	CTGGAG	1 cut(s)	60
BpuEI	CTTGAG	3 cut(s)	228, 1484, 1625
BsaHI	GRCGYC	1 cut(s)	1894
BsaI	GGTCTC	1 cut(s)	1539
BsaJI	CCNNGG	4 cut(s)	95, 677, 1389, 1965
BsaWI	WCCGGW	1 cut(s)	1616
BsaXI	ACNNNNNCTCC	2 cut(s)	1536, 1566
Bsc4I	CCNNNNNNNGG	5 cut(s)	583, 611, 846, 1971, 1991
Bse118I	RCCGGY	5 cut(s)	1504, 1616, 1646, 2086, 2107
Bse1I	ACTGG	8 cut(s)	442, 457, 667, 789, 804, 1119, 1550, 1965
Bse3DI	GCAATG	1 cut(s)	1491
BseBI	CCWGG	1 cut(s)	96
BseDI	CCNNGG	4 cut(s)	95, 677, 1389, 1965
BseGI	GGATG	5 cut(s)	327, 1281, 1471, 1561, 1777
BseLI	CCNNNNNNNGG	5 cut(s)	583, 611, 846, 1971, 1991
BseMI	GCAATG	1 cut(s)	1491
BseMII	CTCAG	5 cut(s)	153, 1064, 1287, 1753, 1808
BseNI	ACTGG	8 cut(s)	442, 457, 667, 789, 804, 1119, 1550, 1965
BseRI	GAGGAG	1 cut(s)	1952
BseXI	GCAGC	7 cut(s)	745, 866, 1831, 1880, 1898, 1935, 2008
BseYI	CCCAGC	2 cut(s)	1166, 1970
Bsh1236I	CGCG	1 cut(s)	2059
BshFI	GGCC	6 cut(s)	250, 1080, 1646, 1964, 1997, 2090
BshNI	GGYRCC	2 cut(s)	1893, 2071
BshTI	ACCGGT	1 cut(s)	1616
BsiHKAI	GWGCWC	1 cut(s)	917
BsiHKCI	CYCGRG	1 cut(s)	474
BsiSI	CCGG	6 cut(s)	1505, 1617, 1647, 1825, 2087, 2108
BslFI	GGGAC	2 cut(s)	1264, 1418
BslI	CCNNNNNNNGG	5 cut(s)	583, 611, 846, 1971, 1991
BsmAI	GTCTC	5 cut(s)	307, 1502, 1539, 1929, 2074
BsmBI	CGTCTC	2 cut(s)	307, 1502
BsmFI	GGGAC	2 cut(s)	1264, 1418
BsmI	GAATGC	1 cut(s)	925
BsnI	GGCC	6 cut(s)	250, 1080, 1646, 1964, 1997, 2090
Bso31I	GGTCTC	1 cut(s)	1539
BsoBI	CYCGRG	1 cut(s)	474
Bsp1286I	GDGCHC	2 cut(s)	917, 1684
Bsp143I	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
BspACI	CCGC	8 cut(s)	166, 1285, 1321, 1576, 1659, 1769, 2059, 2111
BspANI	GGCC	6 cut(s)	250, 1080, 1646, 1964, 1997, 2090
BspCNI	CTCAG	5 cut(s)	154, 1063, 1288, 1752, 1807
BspFNI	CGCG	1 cut(s)	2059
BspLI	GGNNCC	5 cut(s)	1079, 1895, 1996, 2073, 2115
BspMAI	CTGCAG	2 cut(s)	49, 1932
BspPI	GGATC	4 cut(s)	233, 305, 1401, 1682
BspQI	GCTCTTC	1 cut(s)	2127
BspT107I	GGYRCC	2 cut(s)	1893, 2071
BspTNI	GGTCTC	1 cut(s)	1539
BsrBI	CCGCTC	1 cut(s)	166
BsrDI	GCAATG	1 cut(s)	1491
BsrFI	RCCGGY	5 cut(s)	1504, 1616, 1646, 2086, 2107
BsrI	ACTGG	8 cut(s)	442, 457, 667, 789, 804, 1119, 1550, 1965
BssAI	RCCGGY	5 cut(s)	1504, 1616, 1646, 2086, 2107
BssECI	CCNNGG	4 cut(s)	95, 677, 1389, 1965
BssMI	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
BssNAI	GTATAC	1 cut(s)	731
BssNI	GRCGYC	1 cut(s)	1894
BssT1I	CCWWGG	3 cut(s)	677, 1389, 1965
Bst1107I	GTATAC	1 cut(s)	731
Bst2UI	CCWGG	1 cut(s)	96
Bst4CI	ACNGT	6 cut(s)	203, 902, 910, 984, 1496, 1729
Bst6I	CTCTTC	5 cut(s)	481, 1359, 1914, 1929, 2127
BstACI	GRCGYC	1 cut(s)	1894
BstAPI	GCANNNNNTGC	1 cut(s)	758
BstC8I	GCNNGC	5 cut(s)	786, 884, 1289, 1817, 2109
BstDEI	CTNAG	6 cut(s)	162, 1050, 1296, 1397, 1739, 1794
BstF5I	GGATG	5 cut(s)	327, 1281, 1471, 1561, 1777
BstFNI	CGCG	1 cut(s)	2059
BstH2I	RGCGCY	2 cut(s)	1417, 1897
BstHHI	GCGC	3 cut(s)	1314, 1416, 1896
BstKTI	GATC	6 cut(s)	241, 313, 339, 936, 1396, 1690
BstMAI	GTCTC	5 cut(s)	307, 1502, 1539, 1929, 2074
BstMBI	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
BstMWI	GCNNNNNNNGC	5 cut(s)	758, 1318, 1766, 1825, 2113
BstNI	CCWGG	1 cut(s)	96
BstNSI	RCATGY	4 cut(s)	91, 291, 784, 861
BstSCI	CCNGG	1 cut(s)	94
BstSFI	CTRYAG	3 cut(s)	45, 825, 1928
BstUI	CGCG	1 cut(s)	2059
BstV1I	GCAGC	7 cut(s)	745, 866, 1831, 1880, 1898, 1935, 2008
BstV2I	GAAGAC	1 cut(s)	1144
BstX2I	RGATCY	1 cut(s)	1393
BstXI	CCANNNNNNTGG	1 cut(s)	1234
BstYI	RGATCY	1 cut(s)	1393
BstZ17I	GTATAC	1 cut(s)	731
BsuRI	GGCC	6 cut(s)	250, 1080, 1646, 1964, 1997, 2090
BtsCI	GGATG	5 cut(s)	327, 1281, 1471, 1561, 1777
BtsI	GCAGTG	1 cut(s)	1859
BtsIMutI	CAGTG	5 cut(s)	208, 1557, 1747, 1859, 1958
Cac8I	GCNNGC	5 cut(s)	786, 884, 1289, 1817, 2109
CaiI	CAGNNNCTG	1 cut(s)	758
CfoI	GCGC	3 cut(s)	1314, 1416, 1896
Cfr10I	RCCGGY	5 cut(s)	1504, 1616, 1646, 2086, 2107
Cfr13I	GGNCC	3 cut(s)	248, 1078, 1995
Csp6I	GTAC	7 cut(s)	450, 797, 814, 1122, 1508, 2037, 2055
CspAI	ACCGGT	1 cut(s)	1616
CspCI	CAANNNNNGTGG	2 cut(s)	1871, 1906
CviAII	CATG	9 cut(s)	88, 180, 288, 781, 794, 858, 1573, 1677, 2092
CviQI	GTAC	7 cut(s)	450, 797, 814, 1122, 1508, 2037, 2055
DdeI	CTNAG	6 cut(s)	162, 1050, 1296, 1397, 1739, 1794
DinI	GGCGCC	1 cut(s)	1895
DpnI	GATC	6 cut(s)	240, 312, 338, 935, 1395, 1689
DpnII	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
EaeI	YGGCCR	2 cut(s)	1962, 2088
Eam1104I	CTCTTC	5 cut(s)	481, 1359, 1914, 1929, 2127
EarI	CTCTTC	5 cut(s)	481, 1359, 1914, 1929, 2127
EciI	GGCGGA	1 cut(s)	2126
Ecl136II	GAGCTC	1 cut(s)	915
Eco130I	CCWWGG	3 cut(s)	677, 1389, 1965
Eco24I	GRGCYC	2 cut(s)	917, 1684
Eco31I	GGTCTC	1 cut(s)	1539
Eco53kI	GAGCTC	1 cut(s)	915
Eco57I	CTGAAG	2 cut(s)	242, 2084
Eco88I	CYCGRG	1 cut(s)	474
EcoICRI	GAGCTC	1 cut(s)	915
EcoO109I	RGGNCCY	1 cut(s)	1078
EcoRII	CCWGG	1 cut(s)	94
EcoT14I	CCWWGG	3 cut(s)	677, 1389, 1965
EcoT38I	GRGCYC	2 cut(s)	917, 1684
EgeI	GGCGCC	1 cut(s)	1895
EheI	GGCGCC	1 cut(s)	1895
ErhI	CCWWGG	3 cut(s)	677, 1389, 1965
Esp3I	CGTCTC	2 cut(s)	307, 1502
FaeI	CATG	9 cut(s)	91, 183, 291, 784, 797, 861, 1576, 1680, 2095
FaqI	GGGAC	2 cut(s)	1264, 1418
FatI	CATG	9 cut(s)	87, 179, 287, 780, 793, 857, 1572, 1676, 2091
FauI	CCCGC	1 cut(s)	1569
FauNDI	CATATG	3 cut(s)	878, 1126, 1902
FbaI	TGATCA	1 cut(s)	336
FblI	GTMKAC	1 cut(s)	730
Fnu4HI	GCNGC	8 cut(s)	759, 855, 1660, 1820, 1869, 1887, 1949, 2022
FokI	GGATG	5 cut(s)	314, 1288, 1478, 1568, 1784
FriOI	GRGCYC	2 cut(s)	917, 1684
Fsp4HI	GCNGC	8 cut(s)	759, 855, 1660, 1820, 1869, 1887, 1949, 2022
FspBI	CTAG	3 cut(s)	390, 1916, 1991
GlaI	GCGC	3 cut(s)	1313, 1415, 1895
GluI	GCNGC	8 cut(s)	759, 855, 1660, 1820, 1869, 1887, 1949, 2022
GsaI	CCCAGC	2 cut(s)	1170, 1974
GsuI	CTGGAG	1 cut(s)	60
HaeII	RGCGCY	2 cut(s)	1417, 1897
HaeIII	GGCC	6 cut(s)	250, 1080, 1646, 1964, 1997, 2090
HapII	CCGG	6 cut(s)	1505, 1617, 1647, 1825, 2087, 2108
HhaI	GCGC	3 cut(s)	1314, 1416, 1896
Hin1I	GRCGYC	1 cut(s)	1894
Hin1II	CATG	9 cut(s)	91, 183, 291, 784, 797, 861, 1576, 1680, 2095
Hin6I	GCGC	3 cut(s)	1312, 1414, 1894
HinP1I	GCGC	3 cut(s)	1312, 1414, 1894
HindIII	AAGCTT	1 cut(s)	145
HinfI	GANTC	4 cut(s)	152, 1031, 1806, 1922
HpaII	CCGG	6 cut(s)	1505, 1617, 1647, 1825, 2087, 2108
HphI	GGTGA	1 cut(s)	1661
Hpy166II	GTNNAC	3 cut(s)	430, 731, 1097
Hpy188I	TCNGA	8 cut(s)	107, 163, 261, 1053, 1107, 1196, 1633, 2103
Hpy188III	TCNNGA	6 cut(s)	77, 236, 474, 1456, 1685, 1717
Hpy8I	GTNNAC	3 cut(s)	430, 731, 1097
HpyAV	CCTTC	6 cut(s)	266, 477, 578, 1022, 1395, 2056
HpyCH4III	ACNGT	6 cut(s)	203, 902, 910, 984, 1496, 1729
HpyCH4IV	ACGT	3 cut(s)	626, 832, 2014
HpyF10VI	GCNNNNNNNGC	5 cut(s)	758, 1318, 1766, 1825, 2113
HpyF3I	CTNAG	6 cut(s)	162, 1050, 1296, 1397, 1739, 1794
HpySE526I	ACGT	3 cut(s)	626, 832, 2014
Hsp92I	GRCGYC	1 cut(s)	1894
Hsp92II	CATG	9 cut(s)	91, 183, 291, 784, 797, 861, 1576, 1680, 2095
HspAI	GCGC	3 cut(s)	1312, 1414, 1894
KasI	GGCGCC	1 cut(s)	1893
KroI	GCCGGC	1 cut(s)	2107
KroNI	GCCGGC	1 cut(s)	2109
Ksp22I	TGATCA	1 cut(s)	336
Kzo9I	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
LguI	GCTCTTC	1 cut(s)	2127
LmnI	GCTCC	6 cut(s)	469, 755, 803, 1827, 1985, 2113
Lsp1109I	GCAGC	7 cut(s)	745, 866, 1831, 1880, 1898, 1935, 2008
LweI	GCATC	7 cut(s)	496, 997, 1252, 1323, 1471, 1551, 1945
MaeI	CTAG	3 cut(s)	390, 1916, 1991
MaeII	ACGT	3 cut(s)	626, 832, 2014
MaeIII	GTNAC	3 cut(s)	712, 1490, 1649
MalI	GATC	6 cut(s)	240, 312, 338, 935, 1395, 1689
MbiI	CCGCTC	1 cut(s)	166
MboI	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
MfeI	CAATTG	1 cut(s)	195
MflI	RGATCY	1 cut(s)	1393
MhlI	GDGCHC	2 cut(s)	917, 1684
MlsI	TGGCCA	1 cut(s)	1964
MluNI	TGGCCA	1 cut(s)	1964
Mly113I	GGCGCC	1 cut(s)	1894
MlyI	GAGTC	3 cut(s)	1040, 1800, 1931
Mox20I	TGGCCA	1 cut(s)	1964
MroNI	GCCGGC	1 cut(s)	2107
MscI	TGGCCA	1 cut(s)	1964
MseI	TTAA	5 cut(s)	117, 171, 765, 944, 1763
MslI	CAYNNNNRTG	3 cut(s)	332, 1129, 1852
Msp20I	TGGCCA	1 cut(s)	1964
MspI	CCGG	6 cut(s)	1505, 1617, 1647, 1825, 2087, 2108
MspR9I	CCNGG	1 cut(s)	96
MunI	CAATTG	1 cut(s)	195
Mva1269I	GAATGC	1 cut(s)	925
MvaI	CCWGG	1 cut(s)	96
MvnI	CGCG	1 cut(s)	2059
MwoI	GCNNNNNNNGC	5 cut(s)	758, 1318, 1766, 1825, 2113
NaeI	GCCGGC	1 cut(s)	2109
NarI	GGCGCC	1 cut(s)	1894
NdeI	CATATG	3 cut(s)	878, 1126, 1902
NdeII	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
NgoMIV	GCCGGC	1 cut(s)	2107
NlaIII	CATG	9 cut(s)	91, 183, 291, 784, 797, 861, 1576, 1680, 2095
NlaIV	GGNNCC	5 cut(s)	1079, 1895, 1996, 2073, 2115
NmuCI	GTSAC	3 cut(s)	712, 1490, 1649
NspI	RCATGY	4 cut(s)	91, 291, 784, 861
OliI	CACNNNNGTG	1 cut(s)	1852
PaeR7I	CTCGAG	1 cut(s)	474
PciSI	GCTCTTC	1 cut(s)	2127
PcsI	WCGNNNNNNNCGW	1 cut(s)	2063
PctI	GAATGC	1 cut(s)	925
PdiI	GCCGGC	1 cut(s)	2109
PfeI	GAWTC	1 cut(s)	152
PflMI	CCANNNNNTGG	1 cut(s)	846
PinAI	ACCGGT	1 cut(s)	1616
PkrI	GCNGC	8 cut(s)	760, 856, 1661, 1821, 1870, 1888, 1950, 2023
PleI	GAGTC	3 cut(s)	1039, 1800, 1930
PluTI	GGCGCC	1 cut(s)	1897
PpsI	GAGTC	3 cut(s)	1039, 1800, 1930
Psp124BI	GAGCTC	1 cut(s)	917
Psp1406I	AACGTT	1 cut(s)	626
Psp6I	CCWGG	1 cut(s)	94
PspFI	CCCAGC	2 cut(s)	1166, 1970
PspGI	CCWGG	1 cut(s)	94
PspN4I	GGNNCC	5 cut(s)	1079, 1895, 1996, 2073, 2115
PspPI	GGNCC	3 cut(s)	248, 1078, 1995
PstI	CTGCAG	2 cut(s)	49, 1932
PstNI	CAGNNNCTG	1 cut(s)	758
PsuI	RGATCY	1 cut(s)	1393
RsaI	GTAC	7 cut(s)	451, 798, 815, 1123, 1509, 2038, 2056
RsaNI	GTAC	7 cut(s)	450, 797, 814, 1122, 1508, 2037, 2055
RseI	CAYNNNNRTG	3 cut(s)	332, 1129, 1852
SacI	GAGCTC	1 cut(s)	917
SapI	GCTCTTC	1 cut(s)	2127
SaqAI	TTAA	5 cut(s)	117, 171, 765, 944, 1763
SatI	GCNGC	8 cut(s)	759, 855, 1660, 1820, 1869, 1887, 1949, 2022
Sau3AI	GATC	6 cut(s)	238, 310, 336, 933, 1393, 1687
Sau96I	GGNCC	3 cut(s)	248, 1078, 1995
SchI	GAGTC	3 cut(s)	1040, 1800, 1931
ScrFI	CCNGG	1 cut(s)	96
SduI	GDGCHC	2 cut(s)	917, 1684
SfaNI	GCATC	7 cut(s)	496, 997, 1252, 1323, 1471, 1551, 1945
SfcI	CTRYAG	3 cut(s)	45, 825, 1928
SfoI	GGCGCC	1 cut(s)	1895
Sfr274I	CTCGAG	1 cut(s)	474
SlaI	CTCGAG	1 cut(s)	474
SmiMI	CAYNNNNRTG	3 cut(s)	332, 1129, 1852
SmlI	CTYRAG	4 cut(s)	243, 474, 1499, 1640
SmoI	CTYRAG	4 cut(s)	243, 474, 1499, 1640
SsiI	CCGC	8 cut(s)	166, 1285, 1321, 1576, 1659, 1769, 2059, 2111
SspDI	GGCGCC	1 cut(s)	1893
SspI	AATATT	1 cut(s)	999
SspMI	CTAG	3 cut(s)	390, 1916, 1991
SstI	GAGCTC	1 cut(s)	917
StyD4I	CCNGG	1 cut(s)	94
StyI	CCWWGG	3 cut(s)	677, 1389, 1965
TaaI	ACNGT	6 cut(s)	203, 902, 910, 984, 1496, 1729
TaiI	ACGT	3 cut(s)	629, 835, 2017
TaqI	TCGA	5 cut(s)	475, 917, 1455, 1479, 1718
TatI	WGTACW	2 cut(s)	796, 1121
TauI	GCSGC	1 cut(s)	1662
TfiI	GAWTC	1 cut(s)	152
Tru1I	TTAA	5 cut(s)	117, 171, 765, 944, 1763
Tru9I	TTAA	5 cut(s)	117, 171, 765, 944, 1763
TscAI	CASTG	5 cut(s)	208, 1557, 1747, 1859, 1965
TseFI	GTSAC	3 cut(s)	712, 1490, 1649
TseI	GCWGC	7 cut(s)	758, 854, 1819, 1868, 1886, 1948, 2021
Tsp45I	GTSAC	3 cut(s)	712, 1490, 1649
TspGWI	ACGGA	2 cut(s)	703, 1099
TspRI	CASTG	5 cut(s)	208, 1557, 1747, 1859, 1965
Van91I	CCANNNNNTGG	1 cut(s)	846
XapI	RAATTY	8 cut(s)	4, 134, 640, 946, 1055, 1087, 1300, 1369
XceI	RCATGY	4 cut(s)	91, 291, 784, 861
XcmI	CCANNNNNNNNNTGG	2 cut(s)	449, 796
XhoI	CTCGAG	1 cut(s)	474
XmiI	GTMKAC	1 cut(s)	730
XspI	CTAG	3 cut(s)	390, 1916, 1991

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.